FastQCFastQC Report
Fri 27 May 2016
SRR939307_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939307_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2173181
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50410.2319641115949385No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42450.19533577736967145No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26770.12318348080532639No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA80800.030.5734331
GGTATCA55350.030.4420471
ATCAACG98600.024.6915153
TATCAAC101350.024.2088472
TCAACGC100750.024.16464
CAACGCA102250.023.7636955
AACGCAG104750.023.196546
GTACATG73950.022.720861
TACATGG73900.021.8986452
ACATGGG76300.020.7122383
ACGCAGA117750.020.474357
GAGTACT56950.020.12476212-13
CGCAGAG119300.020.0889998
AGAGTAC97600.019.76596610-11
AGTACTT60000.019.49723412-13
GTACTTT59400.019.05501414-15
GCAGAGT129350.018.2713329
TATTCCG2603.314235E-718.2529895
TGCACCG4800.017.7966655
TAGCCCT13300.017.484444
Produced by FastQC (version 0.11.5)