##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939307_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2173181 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.31624977394888 33.0 31.0 34.0 31.0 34.0 2 32.52650009364153 34.0 31.0 34.0 31.0 34.0 3 32.62080240900321 34.0 31.0 34.0 31.0 34.0 4 36.14060494730996 37.0 35.0 37.0 35.0 37.0 5 36.07960956772584 37.0 35.0 37.0 35.0 37.0 6 36.10526136571229 37.0 35.0 37.0 35.0 37.0 7 36.04799278108911 37.0 35.0 37.0 35.0 37.0 8 36.0153111958921 37.0 35.0 37.0 35.0 37.0 9 37.72847222573729 39.0 38.0 39.0 35.0 39.0 10-11 37.65010047483389 39.0 37.0 39.0 35.0 39.0 12-13 37.577361940859966 39.0 37.0 39.0 35.0 39.0 14-15 38.88807927181399 40.0 38.0 41.0 35.0 41.0 16-17 38.881049024448494 40.0 38.0 41.0 35.0 41.0 18-19 38.9695395827591 40.0 38.0 41.0 35.0 41.0 20-21 38.94297713812149 40.0 38.0 41.0 35.0 41.0 22-23 38.86817250841048 40.0 38.0 41.0 35.0 41.0 24-25 38.807746800657654 40.0 38.0 41.0 34.0 41.0 26-27 38.65954906655267 40.0 38.0 41.0 34.0 41.0 28-29 38.49338021085221 40.0 38.0 41.0 34.0 41.0 30-31 38.32112856683359 40.0 38.0 41.0 34.0 41.0 32-33 38.30227003641206 40.0 38.0 41.0 34.0 41.0 34-35 38.22146843728157 40.0 38.0 41.0 34.0 41.0 36-37 38.08658620703936 40.0 38.0 41.0 33.0 41.0 38-39 37.96557396737778 40.0 38.0 41.0 33.0 41.0 40-41 37.79708201939921 40.0 37.0 41.0 33.0 41.0 42-43 37.63017829623948 40.0 37.0 41.0 33.0 41.0 44-45 37.40856836130999 40.0 37.0 41.0 32.0 41.0 46-47 37.141548725117694 40.0 37.0 41.0 31.0 41.0 48-49 36.85711452474506 40.0 36.0 41.0 31.0 41.0 50-51 36.61867971420696 39.0 36.0 41.0 30.5 41.0 52-53 36.363687838242654 39.0 35.0 41.0 30.0 41.0 54-55 36.11708941869085 39.0 35.0 40.0 29.5 41.0 56-57 35.79765744316741 38.5 35.0 40.0 29.0 41.0 58-59 35.477669140306304 38.0 34.5 40.0 28.0 41.0 60-61 35.13793857943724 38.0 34.0 40.0 28.0 41.0 62-63 34.78472225737295 37.0 34.0 40.0 27.0 41.0 64-65 34.468456838155674 37.0 34.0 39.5 26.5 41.0 66-67 34.04791202389492 36.0 33.5 39.0 26.0 40.5 68-69 33.568932822438626 36.0 33.0 39.0 26.0 40.0 70-71 33.08489191650396 35.0 32.0 38.0 25.0 40.0 72-73 32.59564688813311 35.0 32.0 37.5 24.5 39.5 74-75 32.07430605181989 35.0 31.5 37.0 22.5 39.0 76-77 30.128429937497152 33.0 29.5 35.0 20.0 37.0 78-79 31.097812377339945 34.0 31.0 36.0 20.5 38.0 80-81 31.113556809119906 34.0 31.0 35.5 20.5 37.0 82-83 30.764586796958007 34.0 31.0 35.0 20.0 37.0 84-85 30.323163602111375 34.0 30.5 35.0 18.0 36.0 86-87 29.72265517690427 34.0 29.5 35.0 11.5 36.0 88-89 29.14321264542622 34.0 29.0 35.0 7.0 35.5 90-91 28.665622881849234 33.5 29.0 35.0 2.0 35.0 92-93 28.298108164943464 33.0 29.0 35.0 2.0 35.0 94-95 27.776854988148706 33.0 27.0 35.0 2.0 35.0 96-97 27.034699824819008 33.0 26.5 34.5 2.0 35.0 98-99 26.023933349316046 32.0 24.5 34.0 2.0 35.0 100-101 21.69033021179552 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.17689669506228256 1101 2 0.20646421921748725 1101 3 0.22766119699642928 1101 4 0.1423727168324902 1101 5 0.17210061963933043 1101 6 0.20722950723706646 1101 7 0.1695085220864314 1101 8 0.1597653143269966 1101 9 0.26765655077891637 1101 10-11 0.2784617735313404 1101 12-13 0.3626137828629794 1101 14-15 0.3213059366672084 1101 16-17 0.32811693579481016 1101 18-19 0.32319114836852947 1101 20-21 0.3813609809598404 1101 22-23 0.24280226883207234 1101 24-25 0.375030782899195 1101 26-27 0.2853450548774248 1101 28-29 0.2679721754423312 1101 30-31 0.36796814714991655 1101 32-33 0.3925288701479346 1101 34-35 0.49086972398824713 1101 36-37 0.4044332607994434 1101 38-39 0.49068632220029684 1101 40-41 0.42620864855391005 1101 42-43 0.4739909302121461 1101 44-45 0.4363973195876625 1101 46-47 0.3989817823106421 1101 48-49 0.46862956846860726 1101 50-51 0.5524929763688178 1101 52-53 0.42574530604921534 1101 54-55 0.38542091935686074 1101 56-57 0.3166258027067883 1101 58-59 0.2275073655371429 1101 60-61 0.14276874326775868 1101 62-63 0.29678679539883035 1101 64-65 0.19586089814761465 1101 66-67 0.2769255238426993 1101 68-69 0.3376610289209552 1101 70-71 0.23490480161789407 1101 72-73 0.2989472257830883 1101 74-75 0.3028658965001654 1101 76-77 0.4633407029575807 1101 78-79 0.5453466249661041 1101 80-81 0.6989744112023679 1101 82-83 0.45002068864890177 1101 84-85 0.42647713498265105 1101 86-87 0.2098508847294518 1101 88-89 0.5271969140292292 1101 90-91 0.6717073886920524 1101 92-93 0.5034508715171953 1101 94-95 0.17761763823144605 1101 96-97 0.08305740770434866 1101 98-99 0.05451468493777156 1101 100-101 -0.2207437150318441 1102 1 0.2362437036815166 1102 2 0.2320789733158506 1102 3 0.2863554254905978 1102 4 0.1374895412367536 1102 5 0.19609292993170868 1102 6 0.22468450977213905 1102 7 0.20956894121774639 1102 8 0.18370797952896112 1102 9 0.24037815801231233 1102 10-11 0.3038747784324727 1102 12-13 0.2926686037181412 1102 14-15 0.34473736306572533 1102 16-17 0.26238366724655293 1102 18-19 0.29202881948565107 1102 20-21 0.35180947681922703 1102 22-23 0.3487982338481359 1102 24-25 0.3383974594244563 1102 26-27 0.34930007853806444 1102 28-29 0.34019099410043907 1102 30-31 0.31149580052155557 1102 32-33 0.41054792541242335 1102 34-35 0.4075995913196735 1102 36-37 0.5018147463495524 1102 38-39 0.5450117827377241 1102 40-41 0.47992516452484324 1102 42-43 0.4822837728688967 1102 44-45 0.4396844583404089 1102 46-47 0.42978180766132823 1102 48-49 0.4505207295299556 1102 50-51 0.5987991703860587 1102 52-53 0.5218185906529058 1102 54-55 0.4107409717482824 1102 56-57 0.2649420947466794 1102 58-59 0.2626486956030476 1102 60-61 0.4120183630074905 1102 62-63 0.4540170218649493 1102 64-65 0.2688666443027543 1102 66-67 0.2946345613617112 1102 68-69 0.37093538670360715 1102 70-71 0.24870983372876054 1102 72-73 0.33918034645234485 1102 74-75 0.3065158669243715 1102 76-77 0.3829557950650653 1102 78-79 0.3215280724901888 1102 80-81 0.539633212114996 1102 82-83 0.4139683183599061 1102 84-85 0.3448236365881918 1102 86-87 0.06752791700932192 1102 88-89 0.41320069550632965 1102 90-91 0.4959980976661953 1102 92-93 0.43328123318696043 1102 94-95 0.5169312684494649 1102 96-97 0.4563541585916191 1102 98-99 0.4918551868813239 1102 100-101 0.47746906171312986 1103 1 0.174863201616823 1103 2 0.21870551149411455 1103 3 0.25359155602576067 1103 4 0.20039011926904493 1103 5 0.197935744042816 1103 6 0.17269134313167456 1103 7 0.24645263549369645 1103 8 0.2563150106690557 1103 9 0.276316136430232 1103 10-11 0.15684195223937536 1103 12-13 0.25286901686434504 1103 14-15 0.39200944994021114 1103 16-17 0.35380829754587495 1103 18-19 0.3125490692353239 1103 20-21 0.3749010289414372 1103 22-23 0.3488332747282996 1103 24-25 0.2679788670179093 1103 26-27 0.32278189038171945 1103 28-29 0.361078203325782 1103 30-31 0.30217246965703026 1103 32-33 0.27685080481593616 1103 34-35 0.3172715724767272 1103 36-37 0.39135632371154117 1103 38-39 0.3895494913796611 1103 40-41 0.46949655896050047 1103 42-43 0.49711446981778096 1103 44-45 0.4345496117237815 1103 46-47 0.5143270468599468 1103 48-49 0.45911052934955165 1103 50-51 0.409172150169411 1103 52-53 0.3563122382973276 1103 54-55 0.37382805518851114 1103 56-57 0.463801029214288 1103 58-59 0.42668837377519253 1103 60-61 0.42827629698685143 1103 62-63 0.42655919505119755 1103 64-65 0.4260390857754359 1103 66-67 0.14333515242766026 1103 68-69 0.30108494790386686 1103 70-71 0.35148499385653054 1103 72-73 0.49017796418970505 1103 74-75 0.3150489031041417 1103 76-77 0.39164438172026195 1103 78-79 0.4364728915085685 1103 80-81 0.5058945338104444 1103 82-83 0.4575781806996737 1103 84-85 0.5606100315203761 1103 86-87 0.5461123792365434 1103 88-89 0.6672210869711463 1103 90-91 0.6667492034581031 1103 92-93 0.6672226831087578 1103 94-95 0.45382277257472126 1103 96-97 0.19576082209986723 1103 98-99 0.19485773775395998 1103 100-101 -0.19176571787327035 1104 1 0.14863674426909057 1104 2 0.1241069717797103 1104 3 0.1843576996907288 1104 4 0.07595887653825883 1104 5 0.11852960435906112 1104 6 0.1336922524534927 1104 7 0.12292381644676453 1104 8 0.12679122085419436 1104 9 0.2011619569866241 1104 10-11 0.18793282554062074 1104 12-13 0.1782516600270938 1104 14-15 0.12165398172816566 1104 16-17 0.20852867800749664 1104 18-19 0.13101014984272297 1104 20-21 0.1031718467077738 1104 22-23 0.09663541852038549 1104 24-25 0.20993402964263197 1104 26-27 0.22535979679326346 1104 28-29 0.21108888311466956 1104 30-31 0.16551132888805142 1104 32-33 0.2501780628645207 1104 34-35 0.33291789433032903 1104 36-37 0.30768541296821894 1104 38-39 0.2978572831417594 1104 40-41 0.27439295269019226 1104 42-43 0.3105149072087201 1104 44-45 0.3728865801271226 1104 46-47 0.18742573032652388 1104 48-49 0.17922085514100417 1104 50-51 0.18464997525688887 1104 52-53 0.19757793247498512 1104 54-55 0.1817970746853419 1104 56-57 0.14794029858861535 1104 58-59 0.08676596792301439 1104 60-61 0.18027496958887212 1104 62-63 0.18018767488241139 1104 64-65 0.24166725075612305 1104 66-67 0.3124272521184963 1104 68-69 0.28402586366559035 1104 70-71 0.20959851693287845 1104 72-73 0.32206096494677894 1104 74-75 0.35785600985694543 1104 76-77 0.3252922206705193 1104 78-79 0.32951265013302233 1104 80-81 0.4714565875720318 1104 82-83 0.4845989088518685 1104 84-85 0.33018009795679504 1104 86-87 0.3695314933240361 1104 88-89 0.4811277980913573 1104 90-91 0.7589526126635171 1104 92-93 0.7703756474954204 1104 94-95 0.7752702522800412 1104 96-97 0.6825816916145264 1104 98-99 0.7968712782973704 1104 100-101 0.3023096476846945 1105 1 0.10023210296640173 1105 2 0.048966653492072965 1105 3 0.07767374480356892 1105 4 0.02866599421933813 1105 5 0.04985787478142356 1105 6 0.052743415226508716 1105 7 0.11175799256896823 1105 8 0.06896335887673644 1105 9 0.16183838356182179 1105 10-11 0.11962962303796587 1105 12-13 0.11477376238495651 1105 14-15 0.16758751949779338 1105 16-17 0.09213459306901939 1105 18-19 0.06473242061579043 1105 20-21 0.1375668754177255 1105 22-23 0.0857132597073118 1105 24-25 0.14458854798416354 1105 26-27 0.21364688484960936 1105 28-29 0.1452729413604814 1105 30-31 0.21145734097840574 1105 32-33 0.18471259012103047 1105 34-35 0.2433471630310038 1105 36-37 0.20827565030345596 1105 38-39 0.3214610740046666 1105 40-41 0.3909696379234049 1105 42-43 0.3558227092904289 1105 44-45 0.37235481765529954 1105 46-47 0.45115104720991184 1105 48-49 0.43975405275373447 1105 50-51 0.5085309811671053 1105 52-53 0.475079770358235 1105 54-55 0.5714165627582304 1105 56-57 0.47732556881566524 1105 58-59 0.5823811847884812 1105 60-61 0.35879354777291894 1105 62-63 0.5226520921795625 1105 64-65 0.5614006044285418 1105 66-67 0.4611496678313003 1105 68-69 0.5128264090191266 1105 70-71 0.3358057341709255 1105 72-73 0.3031258965185941 1105 74-75 0.4016658242247928 1105 76-77 0.7127770554606165 1105 78-79 0.6520938318242351 1105 80-81 0.6173905767932695 1105 82-83 0.6941515773347966 1105 84-85 0.6029949177349359 1105 86-87 0.6150421635015704 1105 88-89 0.5669019401788589 1105 90-91 0.6497487261879122 1105 92-93 0.7328930462027472 1105 94-95 0.9433099258472666 1105 96-97 0.7559712092883792 1105 98-99 0.9250318553570693 1105 100-101 0.8476144796900442 1106 1 0.049758315651459384 1106 2 0.057414337982329755 1106 3 0.09635625295058503 1106 4 0.025715028951402985 1106 5 0.025522761324566545 1106 6 0.03620445642530257 1106 7 0.05856868811233795 1106 8 0.03618865590448905 1106 9 -0.02327079452069114 1106 10-11 0.04227890015670255 1106 12-13 0.1542629000851008 1106 14-15 0.15290901720691608 1106 16-17 0.11521625748837039 1106 18-19 0.15542539672173916 1106 20-21 0.10295842935222765 1106 22-23 0.17798359948810116 1106 24-25 0.15033320472253564 1106 26-27 0.14124901452186123 1106 28-29 0.13969573495943166 1106 30-31 0.1764751383588674 1106 32-33 0.15957468644197803 1106 34-35 0.32134698725440813 1106 36-37 0.2665919649158113 1106 38-39 0.23657485465860617 1106 40-41 0.4477056977984688 1106 42-43 0.37569159382602635 1106 44-45 0.3945810745157914 1106 46-47 0.27447949272032446 1106 48-49 0.3471135413363484 1106 50-51 0.3850859680185508 1106 52-53 0.4665918422786248 1106 54-55 0.4241811123191326 1106 56-57 0.4784116831583418 1106 58-59 0.4778961061400224 1106 60-61 0.5161819469800832 1106 62-63 0.41848954045845943 1106 64-65 0.3432358277457723 1106 66-67 0.35890204023714745 1106 68-69 0.4111568782732604 1106 70-71 0.158611244304673 1106 72-73 0.19911680239253826 1106 74-75 0.16062846475934123 1106 76-77 0.20311223367727038 1106 78-79 0.33279795586700445 1106 80-81 0.30261204374148676 1106 82-83 0.3006641002609207 1106 84-85 0.2542895355017869 1106 86-87 0.3067874863407347 1106 88-89 0.5097819472099197 1106 90-91 0.6250744332839666 1106 92-93 0.6029268369407106 1106 94-95 0.7648755171157937 1106 96-97 0.6756511339819866 1106 98-99 0.876352723971003 1106 100-101 0.7251735903529806 1107 1 0.07528551302063136 1107 2 0.06921837818237009 1107 3 0.08964263648420001 1107 4 -0.01240080299524493 1107 5 0.08932911974413571 1107 6 0.057359087815228804 1107 7 0.04090170386130154 1107 8 0.06768592716691302 1107 9 0.12781433418879118 1107 10-11 0.10462418754129033 1107 12-13 0.09654055078035384 1107 14-15 0.11633614037550188 1107 16-17 0.12895873315365236 1107 18-19 0.09122619229253104 1107 20-21 0.1301542618257443 1107 22-23 0.13707690343390055 1107 24-25 0.026684840867439164 1107 26-27 0.2037140546296925 1107 28-29 0.32625675725913084 1107 30-31 0.20043808636138039 1107 32-33 0.09989161954290893 1107 34-35 0.18989548765613762 1107 36-37 0.17214197128213016 1107 38-39 0.16203948644367472 1107 40-41 0.21885495074344163 1107 42-43 0.24740547372644528 1107 44-45 0.2799468746308307 1107 46-47 0.15226464909037674 1107 48-49 0.18782035681397957 1107 50-51 0.20511507747978186 1107 52-53 0.2145565948042929 1107 54-55 0.27671567706023836 1107 56-57 0.3830691969557023 1107 58-59 0.2800904019149826 1107 60-61 0.3651523589470145 1107 62-63 0.26253702883443 1107 64-65 0.058369235133341135 1107 66-67 0.2259874938486064 1107 68-69 0.2740884161572694 1107 70-71 0.24184498493630002 1107 72-73 0.34261064023132803 1107 74-75 0.16962276141241261 1107 76-77 0.23335994948462968 1107 78-79 0.3712779853716164 1107 80-81 0.33176782546004446 1107 82-83 0.43523992755356744 1107 84-85 0.5475173049618789 1107 86-87 0.45613735308701564 1107 88-89 0.6727368648648344 1107 90-91 0.6970256233472334 1107 92-93 0.6099075096634294 1107 94-95 0.789609558501283 1107 96-97 0.7900528533281452 1107 98-99 0.8163084231468929 1107 100-101 0.968340550455892 1108 1 -0.041391627796421915 1108 2 -0.0015475828705717731 1108 3 -0.008183550793603445 1108 4 0.03006733195432787 1108 5 0.0534395623182462 1108 6 0.05512553467855952 1108 7 0.037682895363737146 1108 8 0.005150005306028049 1108 9 0.0677370408323128 1108 10-11 -0.012375463413590637 1108 12-13 0.004799579176342661 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-0.31449821169474035 2316 84-85 -0.3379413753845455 2316 86-87 -0.4713144112279224 2316 88-89 -0.7491467671939844 2316 90-91 -0.8546256743605731 2316 92-93 -0.59994887364671 2316 94-95 -0.4893501983645585 2316 96-97 -0.36113927942643187 2316 98-99 -0.18041909628930242 2316 100-101 0.31540729465965 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 9.0 4 14.0 5 53.0 6 85.0 7 230.0 8 546.0 9 1000.0 10 1612.0 11 2571.0 12 3713.0 13 5036.0 14 6442.0 15 8625.0 16 10997.0 17 15039.0 18 15070.0 19 11053.0 20 10627.0 21 11762.0 22 12915.0 23 14196.0 24 16418.0 25 19493.0 26 23724.0 27 28772.0 28 35127.0 29 42961.0 30 52592.0 31 64009.0 32 79897.0 33 103814.0 34 141625.0 35 204377.0 36 326819.0 37 513206.0 38 367147.0 39 21603.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.15009756304677 19.243826391311813 15.13394843280703 23.472127612834388 2 15.841018304503859 20.49327690606535 38.76308508127026 24.902619708160525 3 19.41945930872762 23.75908863550712 29.634853240480197 27.186598815285056 4 13.431692988296879 16.946080423121682 36.373224319557366 33.24900226902407 5 14.385502174002074 36.85914794948051 33.928144963535026 14.827204912982397 6 32.61845193750544 35.76098815515137 17.154576632135104 14.465983275208094 7 29.172351497643316 32.097740593167345 20.272494559818075 18.45741334937127 8 26.640624964050396 34.83722708784956 19.58755391290463 18.934594035195413 9 26.294496408720676 16.820780229534492 19.99216816270711 36.89255519903772 10-11 25.33353181350288 26.460773400835 26.91395700588216 21.29173777977996 12-13 25.84142784241165 23.568883585858703 27.49170455659239 23.097984015137257 14-15 22.872061269486945 25.105106473150656 25.61501062856351 26.40782162879889 16-17 22.681013186536585 28.310640225643404 26.287460653558743 22.720885934261272 18-19 22.653594581766217 26.98197411581781 28.29875383107846 22.065677471337516 20-21 23.362831573355272 26.11722772222556 28.24461658845181 22.275324115967358 22-23 23.029052803240962 25.986606729950246 28.23087446466723 22.75346600214156 24-25 22.88831441099476 26.154839380613026 28.317728711966467 22.639117496425744 26-27 22.67109090620448 26.441327097002343 28.2305718418566 22.65701015493658 28-29 22.659479353077355 26.644191164932877 28.222568667773185 22.47376081421658 30-31 22.759286962291682 26.320334109307968 28.077458803477484 22.842920124922866 32-33 22.63210013339892 26.33096368871254 28.11270667284501 22.924229505043527 34-35 23.13017781294427 26.478720219846313 27.851540237935225 22.539561729274197 36-37 23.056005835163532 26.214127032154373 28.049120296175968 22.680746836506128 38-39 22.779456749446936 26.490982445766402 28.032197908769525 22.697362896017136 40-41 22.993793464443282 26.29636063480377 27.939694792607376 22.770151108145576 42-43 22.827297117649778 26.31061150404627 27.70375779763659 23.15833358066736 44-45 22.92132003287382 26.499936958104286 27.627052427849243 22.951690581172652 46-47 22.993944578362598 26.659845622295293 27.412177519865505 22.93403227947661 48-49 23.074059485397385 26.565890780849376 27.386971670903176 22.973078062850064 50-51 23.055724087345713 26.772189424670433 27.29377518342356 22.8783113045603 52-53 23.198009368759145 26.763981814667908 27.071733588566065 22.966275228006882 54-55 22.973028937764504 26.690160644695492 27.138673676973983 23.19813674056602 56-57 22.97411652968507 26.919862891094688 27.173847520871654 22.932173058348592 58-59 23.13082768214304 26.63156693468118 27.13880507523442 23.09880030794136 60-61 23.011619664273773 26.678959004348258 27.44001330775165 22.86940802362632 62-63 22.86986890279747 26.69745441945445 27.465161883037986 22.967514794710095 64-65 22.68770251145844 26.59416766999689 27.361384551989374 23.356745266555293 66-67 22.657192961628 26.786431637057888 27.191530540826708 23.36484486048741 68-69 22.794842232437297 26.59609934797399 27.386012789271973 23.223045630316737 70-71 22.800493838295107 26.576019208708342 27.245958804167714 23.377528148828837 72-73 22.87057543757285 26.564377288408096 27.227161474354872 23.33788579966418 74-75 22.740195763766515 26.872917366965147 26.90057268597799 23.486314183290343 76-77 22.82166807530624 26.605595374741185 27.05766718214263 23.51506936780994 78-79 22.8068702133764 26.719043097472206 27.079597925342995 23.3944887638084 80-81 22.834377003832405 26.816560712081078 26.953456904963442 23.395605379123076 82-83 22.920571273170527 26.741444914160397 26.940484938898322 23.397498873770754 84-85 22.79517904859282 26.668602385167183 26.951367603526812 23.584850962713183 86-87 22.749471752400087 26.699017945447352 26.9727807957871 23.57872950636546 88-89 22.713438678128732 26.624020691765438 26.875254750041854 23.78728588006397 90-91 22.622449763534068 26.655163479757878 26.994352918051213 23.728033838656845 92-93 22.666956871056758 26.510562166704016 26.998004307970664 23.824476654268558 94-95 22.52239919270415 26.48129631172001 27.023772985315077 23.972531510260765 96-97 22.586820886065173 26.41248013856186 27.047609011858654 23.953089963514316 98-99 22.47071396607042 26.448759937924997 26.782073714641346 24.298452381363234 100-101 22.595718246344635 25.841959345197697 27.38799222335983 24.17433018509784 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 172.0 1 122.5 2 115.0 3 163.0 4 145.5 5 139.0 6 258.5 7 592.5 8 967.5 9 1216.0 10 1242.5 11 1187.0 12 1298.5 13 1469.0 14 1626.0 15 1740.0 16 1887.5 17 2086.0 18 2251.5 19 2388.0 20 2671.0 21 3170.0 22 3774.0 23 4724.0 24 5794.5 25 7192.5 26 9209.0 27 11778.5 28 14378.0 29 17221.0 30 20703.5 31 24664.5 32 28997.0 33 32499.5 34 35903.0 35 39791.5 36 44388.0 37 49269.0 38 53321.5 39 56249.0 40 59324.5 41 64325.5 42 69209.0 43 73208.5 44 77193.5 45 80258.5 46 81346.5 47 84402.5 48 90985.0 49 92296.5 50 88849.5 51 86415.5 52 83993.0 53 82972.5 54 82110.5 55 76135.5 56 67533.5 57 60996.5 58 55500.0 59 48421.0 60 40030.0 61 32470.0 62 26124.0 63 20353.0 64 15402.0 65 11775.5 66 8908.5 67 6883.0 68 5147.5 69 3607.5 70 2648.0 71 1981.5 72 1563.5 73 1298.5 74 1002.5 75 737.0 76 479.0 77 251.0 78 128.5 79 86.5 80 59.5 81 27.5 82 20.0 83 14.0 84 7.5 85 6.0 86 4.5 87 3.0 88 1.5 89 1.0 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.293440813259457 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0012654261195915112 16-17 8.052711670127799E-4 18-19 2.3007747628936568E-5 20-21 2.3007747628936568E-5 22-23 0.0 24-25 0.0 26-27 6.902324288680971E-5 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0012194106243336382 36-37 0.006833301045794161 38-39 0.0026919064725855787 40-41 0.00315206142516431 42-43 2.991007191761754E-4 44-45 0.001058356390931082 46-47 2.070697286604291E-4 48-49 4.141394573208582E-4 50-51 2.3007747628936568E-5 52-53 4.6015495257873136E-5 54-55 0.0 56-57 8.282789146417164E-4 58-59 0.0018406198103149253 60-61 5.29178195465541E-4 62-63 0.0010353486433021457 64-65 6.902324288680971E-5 66-67 3.681239620629851E-4 68-69 0.0014494881006230037 70-71 0.0 72-73 0.0 74-75 2.3007747628936568E-5 76-77 0.2579168509203789 78-79 1.1503873814468283E-4 80-81 6.902324288680971E-5 82-83 0.0 84-85 0.0 86-87 0.10694001097929717 88-89 0.37127602348814936 90-91 0.27404528200826345 92-93 0.0 94-95 0.0 96-97 0.0 98-99 1.6105423340255598E-4 100-101 2.7609297154723884E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2173181.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.90732509620507 #Duplication Level Percentage of deduplicated Percentage of total 1 72.66832783124357 38.44686844765211 2 14.82273992351391 15.684630398996966 3 5.346261307893103 8.485691551978888 4 2.438204933439973 5.159956042587189 5 1.3082362435830412 3.4607640120943044 6 0.7921942312010282 2.5147726637694623 7 0.5401673164689473 2.00051654731371 8 0.3577127967313383 1.5140501782190114 9 0.2565932492335317 1.2218096209221032 >10 1.3315669049847207 12.826108063021794 >50 0.09232234264863681 3.329723682238004 >100 0.04340850054448811 4.012010134389628 >500 0.0017418603952140599 0.6001735969891808 >1k 4.354650988034932E-4 0.510642902252118 >5k 8.709301976069864E-5 0.23228215757561146 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5041 0.2319641115949385 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4245 0.19533577736967145 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2677 0.12318348080532639 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.3007747628936568E-5 0.0 12-13 0.0 0.0 0.0 6.902324288680971E-5 1.1503873814468285E-4 14-15 0.0 0.0 0.0 1.3804648577361942E-4 1.6105423340255598E-4 16-17 0.0 0.0 0.0 1.3804648577361942E-4 2.300774762893657E-4 18-19 0.0 0.0 0.0 1.3804648577361942E-4 2.7609297154723884E-4 20-21 0.0 0.0 0.0 1.3804648577361942E-4 2.7609297154723884E-4 22-23 0.0 0.0 0.0 2.300774762893657E-4 2.7609297154723884E-4 24-25 0.0 0.0 0.0 2.7609297154723884E-4 2.7609297154723884E-4 26-27 0.0 0.0 0.0 5.061704478366045E-4 2.7609297154723884E-4 28-29 0.0 0.0 0.0 0.001702573324541306 3.2210846680511197E-4 30-31 0.0 0.0 0.0 0.0044174875447558215 3.2210846680511197E-4 32-33 0.0 0.0 0.0 0.011043718861889554 3.2210846680511197E-4 34-35 0.0 0.0 0.0 0.021834352499860803 3.2210846680511197E-4 36-37 0.0 0.0 0.0 0.042035154918067114 3.2210846680511197E-4 38-39 0.0 0.0 0.0 0.07551142771816982 3.911317096919216E-4 40-41 0.0 0.0 0.0 0.11575197832117987 4.141394573208582E-4 42-43 0.0 0.0 0.0 0.15850037341574402 4.141394573208582E-4 44-45 0.0 0.0 0.0 0.21203940214827943 4.371472049497948E-4 46-47 0.0 0.0 0.0 0.27738140541445927 4.83162700207668E-4 48-49 0.0 0.0 0.0 0.34868241531653366 5.061704478366045E-4 50-51 0.0 0.0 0.0 0.4275759819361572 5.521859430944777E-4 52-53 0.0 0.0 0.0 0.5079650521516615 5.521859430944777E-4 54-55 0.0 0.0 0.0 0.6018826779729807 5.521859430944777E-4 56-57 0.0 0.0 0.0 0.7161621604459085 5.521859430944777E-4 58-59 0.0 0.0 0.0 0.8432569583481542 5.521859430944777E-4 60-61 0.0 0.0 0.0 0.9801760644879557 5.521859430944777E-4 62-63 0.0 0.0 0.0 1.1234913244686016 5.521859430944777E-4 64-65 0.0 0.0 0.0 1.2808413105028986 5.521859430944777E-4 66-67 0.0 0.0 0.0 1.4629016174906737 5.751936907234142E-4 68-69 0.0 0.0 0.0 1.671305795513581 6.212091859812874E-4 70-71 0.0 0.0 0.0 1.9046733797138848 7.132401764970335E-4 72-73 4.6015495257873136E-5 0.0 0.0 2.1424584514589444 7.592556717549068E-4 74-75 4.6015495257873136E-5 0.0 0.0 2.390574001889396 8.282789146417164E-4 76-77 4.6015495257873136E-5 0.0 0.0 2.6672881826226162 8.282789146417164E-4 78-79 4.6015495257873136E-5 0.0 0.0 2.980906790552651 8.282789146417164E-4 80-81 4.6015495257873136E-5 0.0 0.0 3.326069020481957 8.282789146417164E-4 82-83 4.6015495257873136E-5 0.0 0.0 3.6848058215123363 8.282789146417164E-4 84-85 4.6015495257873136E-5 0.0 0.0 4.063490339737003 8.282789146417164E-4 86-87 4.6015495257873136E-5 0.0 0.0 4.4598218003930645 8.742944098995896E-4 88-89 4.6015495257873136E-5 0.0 0.0 4.896071703185331 9.203099051574628E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 8080 0.0 30.573433 1 GGTATCA 5535 0.0 30.442047 1 ATCAACG 9860 0.0 24.691515 3 TATCAAC 10135 0.0 24.208847 2 TCAACGC 10075 0.0 24.1646 4 CAACGCA 10225 0.0 23.763695 5 AACGCAG 10475 0.0 23.19654 6 GTACATG 7395 0.0 22.72086 1 TACATGG 7390 0.0 21.898645 2 ACATGGG 7630 0.0 20.712238 3 ACGCAGA 11775 0.0 20.47435 7 GAGTACT 5695 0.0 20.124762 12-13 CGCAGAG 11930 0.0 20.088999 8 AGAGTAC 9760 0.0 19.765966 10-11 AGTACTT 6000 0.0 19.497234 12-13 GTACTTT 5940 0.0 19.055014 14-15 GCAGAGT 12935 0.0 18.271332 9 TATTCCG 260 3.314235E-7 18.252989 5 TGCACCG 480 0.0 17.796665 5 TAGCCCT 1330 0.0 17.48444 4 >>END_MODULE