FastQCFastQC Report
Fri 27 May 2016
SRR939306_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939306_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2211291
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50960.23045361284426158No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38800.17546311182019916No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25640.11595036564613159No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA88150.030.6595021
GGTATCA58900.027.8213981
ATCAACG104400.025.2529073
TCAACGC105100.025.1298314
CAACGCA105500.024.9446625
TATCAAC106000.024.9164622
AACGCAG107700.024.3332886
GTACATG75000.022.73571
TACATGG75150.022.1470152
ACGCAGA118350.021.9834277
CGCAGAG119400.021.7504168
GAGTACT52000.021.23507312-13
ACATGGG77600.020.7145273
AGAGTAC93600.020.3791610-11
AGTACTT57600.019.99332212-13
GAGTACA63500.019.8219931
GCAGAGT127850.019.6456539
GTGTAGC13700.019.4152341
GTACTTT57000.019.41391814-15
AGTACAT60650.018.450932
Produced by FastQC (version 0.11.5)