##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939306_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2211291 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.741235323618646 33.0 31.0 34.0 30.0 34.0 2 31.829371620469672 33.0 31.0 34.0 30.0 34.0 3 31.88608735801846 34.0 31.0 34.0 30.0 34.0 4 35.3817457765622 37.0 35.0 37.0 33.0 37.0 5 35.42254230673394 37.0 35.0 37.0 33.0 37.0 6 35.50690705112986 37.0 35.0 37.0 33.0 37.0 7 35.415493483218626 37.0 35.0 37.0 33.0 37.0 8 35.39467170987446 37.0 35.0 37.0 33.0 37.0 9 37.00522002757665 39.0 37.0 39.0 33.0 39.0 10-11 36.90514749076445 39.0 37.0 39.0 33.0 39.0 12-13 36.796982396256304 39.0 37.0 39.0 33.0 39.0 14-15 38.02394867975314 40.0 38.0 41.0 33.0 41.0 16-17 37.95166782662255 40.0 38.0 41.0 33.0 41.0 18-19 37.870110039791236 40.0 38.0 41.0 32.0 41.0 20-21 37.76041597419788 40.0 38.0 41.0 32.0 41.0 22-23 37.67996636354057 40.0 37.5 41.0 32.0 41.0 24-25 37.71205553678824 40.0 38.0 41.0 32.0 41.0 26-27 37.62945695523565 40.0 38.0 41.0 32.0 41.0 28-29 37.49123294943994 40.0 37.0 41.0 31.5 41.0 30-31 37.32623114732525 40.0 37.0 41.0 31.0 41.0 32-33 37.14369094795755 40.0 37.0 41.0 30.5 41.0 34-35 36.84438999661284 40.0 36.0 41.0 30.0 41.0 36-37 36.61338806154414 40.0 36.0 41.0 30.0 41.0 38-39 36.29464213439118 39.0 35.5 41.0 29.5 41.0 40-41 36.165541758185604 39.0 35.0 41.0 29.5 41.0 42-43 36.013649266424004 39.0 35.0 41.0 28.5 41.0 44-45 36.007688947316296 39.0 35.0 41.0 28.0 41.0 46-47 35.85245293360304 39.0 35.0 41.0 27.5 41.0 48-49 35.67586491330178 39.0 35.0 41.0 27.0 41.0 50-51 34.53453638621059 38.0 34.0 40.0 25.0 40.5 52-53 34.646657540775955 38.0 34.0 39.5 25.0 40.5 54-55 35.14792082995861 39.0 35.0 40.5 26.0 41.0 56-57 35.036811527745556 39.0 35.0 41.0 26.0 41.0 58-59 34.828352984749635 38.0 34.0 40.0 26.0 41.0 60-61 34.532381988621125 38.0 34.0 40.0 25.0 41.0 62-63 34.16639374917187 37.5 34.0 40.0 23.5 41.0 64-65 33.78739387986475 37.0 33.5 40.0 23.0 41.0 66-67 33.42248442199602 36.5 33.0 39.5 22.0 41.0 68-69 32.99901980336374 36.0 33.0 39.0 21.5 41.0 70-71 32.58567189935653 35.5 32.0 39.0 20.0 40.0 72-73 32.14136153948078 35.0 32.0 38.0 18.5 40.0 74-75 31.689395018566078 35.0 31.5 37.0 18.0 39.0 76-77 31.23798134212096 35.0 31.0 36.5 14.5 39.0 78-79 30.810574004054644 35.0 31.0 36.0 9.5 39.0 80-81 30.354644413602735 34.5 30.5 36.0 7.0 37.5 82-83 29.914016291840376 34.0 30.0 35.0 4.0 37.0 84-85 29.497285748460968 34.0 29.5 35.0 2.0 36.5 86-87 29.157969710906436 34.0 29.0 35.0 2.0 36.0 88-89 28.7461855992721 34.0 29.0 35.0 2.0 36.0 90-91 28.342342324008918 34.0 29.0 35.0 2.0 35.0 92-93 27.9208091562802 34.0 27.5 35.0 2.0 35.0 94-95 27.436832827520213 34.0 27.0 35.0 2.0 35.0 96-97 26.852546317965388 33.5 25.5 35.0 2.0 35.0 98-99 26.094826280213685 33.0 24.5 35.0 2.0 35.0 100-101 23.58829841933965 31.0 10.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.46801144059154254 1101 2 0.2630164806102222 1101 3 0.4560028784760668 1101 4 0.5203465978316402 1101 5 0.4343060633596778 1101 6 0.4187764305095527 1101 7 0.46000054718669503 1101 8 0.4581204178550351 1101 9 0.4925484291041329 1101 10-11 0.5750059505623639 1101 12-13 0.595275662395764 1101 14-15 0.6102301528126048 1101 16-17 0.5832797671825816 1101 18-19 0.6520698343306819 1101 20-21 0.5948805970267088 1101 22-23 0.6348099635578635 1101 24-25 0.5791144293395902 1101 26-27 0.6187698773054322 1101 28-29 0.5529278255257495 1101 30-31 0.5681545847099088 1101 32-33 0.5400181980313619 1101 34-35 0.5548131257812798 1101 36-37 0.5868373466628682 1101 38-39 0.6633812396577952 1101 40-41 0.5885727628289814 1101 42-43 0.45597726990984455 1101 44-45 0.5555875623483857 1101 46-47 0.5582468321686491 1101 48-49 0.5910018298842701 1101 50-51 0.6152005736060744 1101 52-53 0.7623678713362878 1101 54-55 0.7507026745724659 1101 56-57 0.5782555048187561 1101 58-59 0.5868683375091308 1101 60-61 0.6556053546447771 1101 62-63 0.6688387017757265 1101 64-65 0.6499926118868657 1101 66-67 0.5067268805341598 1101 68-69 0.6588176468025253 1101 70-71 0.7159241337194899 1101 72-73 0.6054821449650234 1101 74-75 0.6720808914521861 1101 76-77 0.7044049231211424 1101 78-79 0.6212600310351348 1101 80-81 0.4921345525014047 1101 82-83 0.5169682762849099 1101 84-85 0.4183554372361016 1101 86-87 0.4538550218559507 1101 88-89 0.4615320285520035 1101 90-91 0.5184874590754163 1101 92-93 0.4149978324274244 1101 94-95 0.4640480704607022 1101 96-97 0.287070407342199 1101 98-99 0.31141764778679715 1101 100-101 0.23046619717859684 1102 1 0.4075301655299235 1102 2 0.3745199938353956 1102 3 0.4605078386840411 1102 4 0.3473394180992102 1102 5 0.39534712456215004 1102 6 0.3862492076128916 1102 7 0.3176513154364642 1102 8 0.40146812904708185 1102 9 0.5942234956285546 1102 10-11 0.5375947775252996 1102 12-13 0.5280856140812489 1102 14-15 0.6724017459651819 1102 16-17 0.7246843981406528 1102 18-19 0.6027030425858371 1102 20-21 0.7270610958679029 1102 22-23 0.5317917628441862 1102 24-25 0.5859454450479333 1102 26-27 0.6354462007428765 1102 28-29 0.6519706556033711 1102 30-31 0.6711612892679071 1102 32-33 0.6642395250862592 1102 34-35 0.7163245330333652 1102 36-37 0.7010489709623684 1102 38-39 0.7670137182902721 1102 40-41 0.7185923676064689 1102 42-43 0.7307430548980136 1102 44-45 0.8880165314344524 1102 46-47 0.8221935850411626 1102 48-49 0.8933668190318897 1102 50-51 0.9174793415860591 1102 52-53 1.0778683679702183 1102 54-55 1.012648329945307 1102 56-57 1.0844101633676075 1102 58-59 0.8951852696603524 1102 60-61 0.8552928124922019 1102 62-63 0.8120271528910052 1102 64-65 0.9982414408118103 1102 66-67 1.0677427268351565 1102 68-69 1.0743791497026152 1102 70-71 0.889468183922439 1102 72-73 0.8227050722847906 1102 74-75 0.7229023300964421 1102 76-77 0.5780809491913566 1102 78-79 0.5697967236984454 1102 80-81 0.611167281290232 1102 82-83 0.451379259252203 1102 84-85 0.3919273081358057 1102 86-87 0.26457196810912365 1102 88-89 0.40624088457721896 1102 90-91 0.40604241069959457 1102 92-93 0.6358585266112868 1102 94-95 0.7163158787722672 1102 96-97 0.4240988350359771 1102 98-99 0.5362134760618211 1102 100-101 0.2573020566005404 1103 1 0.22364091430138444 1103 2 0.24021777795753607 1103 3 0.3589402753292923 1103 4 0.29406369676984667 1103 5 0.2855218064552858 1103 6 0.2586665121340701 1103 7 0.21392868575305357 1103 8 0.320970787116039 1103 9 0.39905642286801424 1103 10-11 0.5009014788945336 1103 12-13 0.4626268203031003 1103 14-15 0.5690013763787505 1103 16-17 0.5179895407657469 1103 18-19 0.4255051107932033 1103 20-21 0.4759992014194907 1103 22-23 0.48096875619081203 1103 24-25 0.5798617281815055 1103 26-27 0.47402029851715355 1103 28-29 0.3842289169473432 1103 30-31 0.41884701568368854 1103 32-33 0.48964274758468207 1103 34-35 0.4214216776602058 1103 36-37 0.5314670938242259 1103 38-39 0.5993800149603032 1103 40-41 0.6237861133506897 1103 42-43 0.5867776508140992 1103 44-45 0.5071886742154206 1103 46-47 0.4560684281412577 1103 48-49 0.5627566732482947 1103 50-51 0.5636339095267857 1103 52-53 0.49948609827466584 1103 54-55 0.5932033556645848 1103 56-57 0.6306625630131961 1103 58-59 0.6251743789611339 1103 60-61 0.6673754382377339 1103 62-63 0.5772300357472773 1103 64-65 0.5597392811307813 1103 66-67 0.4237432252230846 1103 68-69 0.49817604534059967 1103 70-71 0.3900806575673528 1103 72-73 0.3919763799070495 1103 74-75 0.3522047715820058 1103 76-77 0.390058205883701 1103 78-79 0.38609693166772985 1103 80-81 0.43171686165990053 1103 82-83 0.3756835158543055 1103 84-85 0.1590196555224459 1103 86-87 0.3261205701617449 1103 88-89 0.41464118237938763 1103 90-91 0.3997871807027913 1103 92-93 0.32874721792251904 1103 94-95 0.1431538637262868 1103 96-97 -0.013607031994943952 1103 98-99 -0.15714976054733754 1103 100-101 -0.29596956277054787 1104 1 0.12338037148397518 1104 2 0.2236871708838315 1104 3 0.22669144402082608 1104 4 0.22194987672225608 1104 5 0.1764736572085681 1104 6 0.22331958625611747 1104 7 0.22085065854511043 1104 8 0.2882451882873909 1104 9 0.3526207767948648 1104 10-11 0.3611952056455294 1104 12-13 0.32888473682506003 1104 14-15 0.27162483983940433 1104 16-17 0.3821135301313845 1104 18-19 0.33021246963726725 1104 20-21 0.40912853231035484 1104 22-23 0.5306640443107256 1104 24-25 0.44524595182701177 1104 26-27 0.33583634256896033 1104 28-29 0.3794504354787236 1104 30-31 0.4640509957683676 1104 32-33 0.5191244790318166 1104 34-35 0.6278269618501184 1104 36-37 0.5516148192168728 1104 38-39 0.7067601486201625 1104 40-41 0.6352211309214297 1104 42-43 0.7031569204694534 1104 44-45 0.5775272854784745 1104 46-47 0.6437247153266128 1104 48-49 0.5980877002510496 1104 50-51 0.6000507800220021 1104 52-53 0.7752630191693157 1104 54-55 0.8745243882369138 1104 56-57 0.822851892781209 1104 58-59 0.7879833326114394 1104 60-61 0.8510904191019506 1104 62-63 0.7505276512637877 1104 64-65 0.821234664743308 1104 66-67 0.898081695383631 1104 68-69 0.9393936805717118 1104 70-71 0.7459244382006318 1104 72-73 0.607949184881015 1104 74-75 0.5920547060736645 1104 76-77 0.7118944166650287 1104 78-79 0.5853464799160939 1104 80-81 0.3897156354591509 1104 82-83 0.4638704776835887 1104 84-85 0.3939936037249048 1104 86-87 0.28128884733478543 1104 88-89 0.31511447202062115 1104 90-91 0.45698263899035396 1104 92-93 0.5487705097746911 1104 94-95 0.4798402449982042 1104 96-97 0.5399783879415523 1104 98-99 0.23643173932274308 1104 100-101 -0.11080703900297095 1105 1 0.35192365793176705 1105 2 0.3496782968428569 1105 3 0.3552235802644397 1105 4 0.31695294481149006 1105 5 0.46396823961202216 1105 6 0.40089027296367163 1105 7 0.4804587942476104 1105 8 0.43046687479262147 1105 9 0.5672324893592418 1105 10-11 0.5351877228837694 1105 12-13 0.5799704552678975 1105 14-15 0.5954799052535407 1105 16-17 0.48213668641896845 1105 18-19 0.6316659665101056 1105 20-21 0.6116826898506176 1105 22-23 0.6501157775968878 1105 24-25 0.6300246868422832 1105 26-27 0.6503129926387032 1105 28-29 0.7159009522276776 1105 30-31 0.7325722813655133 1105 32-33 0.6197394995693486 1105 34-35 0.585703083319352 1105 36-37 0.7079999990977157 1105 38-39 0.8885129940024896 1105 40-41 0.8288628517997267 1105 42-43 0.7100159602538128 1105 44-45 0.7660780703791588 1105 46-47 0.781427002972876 1105 48-49 0.6866089097700367 1105 50-51 0.5579694577076424 1105 52-53 0.6227594892619237 1105 54-55 0.7238639704394743 1105 56-57 0.7844081759925956 1105 58-59 0.7712753816599758 1105 60-61 0.7819127930208367 1105 62-63 0.8284997928709004 1105 64-65 0.758451763973703 1105 66-67 0.7547668609285054 1105 68-69 0.7513620101879113 1105 70-71 0.5713690615079869 1105 72-73 0.7653987456604483 1105 74-75 0.7673451879122659 1105 76-77 0.6377280846233582 1105 78-79 0.5773645741536271 1105 80-81 0.6175622124534499 1105 82-83 0.6902223567307395 1105 84-85 0.5885829730869503 1105 86-87 0.4774746691725902 1105 88-89 0.6842945230928379 1105 90-91 0.67014946102287 1105 92-93 0.7734026659650439 1105 94-95 0.6818869374886489 1105 96-97 0.6389570149734034 1105 98-99 0.5735373602856946 1105 100-101 0.41563867769007246 1106 1 0.34635768234449316 1106 2 0.4791099024055505 1106 3 0.384887819270066 1106 4 0.3505260819266809 1106 5 0.2519256705859263 1106 6 0.305645005216455 1106 7 0.4228452729329035 1106 8 0.28654176594437075 1106 9 0.35064526230020476 1106 10-11 0.2990171532816177 1106 12-13 0.4053815515344894 1106 14-15 0.46966563693231933 1106 16-17 0.36806232894095814 1106 18-19 0.5046732636212212 1106 20-21 0.5944410630252861 1106 22-23 0.5930264733802986 1106 24-25 0.5632680719197936 1106 26-27 0.638299066989795 1106 28-29 0.6112748075465007 1106 30-31 0.45628161466299133 1106 32-33 0.48869604891058316 1106 34-35 0.40360571816824375 1106 36-37 0.5261415566274934 1106 38-39 0.6257324535427529 1106 40-41 0.6170854106126455 1106 42-43 0.4408403357905968 1106 44-45 0.6818571987226747 1106 46-47 0.6313128054511594 1106 48-49 0.5042794580662573 1106 50-51 0.44987101563847887 1106 52-53 0.32399516500977654 1106 54-55 0.43665794389114865 1106 56-57 0.6054109949839983 1106 58-59 0.47876919097620174 1106 60-61 0.4722857471100781 1106 62-63 0.38712825135361584 1106 64-65 0.39577385658547115 1106 66-67 0.3767173495863432 1106 68-69 0.4285820257332702 1106 70-71 0.5494594984710162 1106 72-73 0.6270486133016107 1106 74-75 0.40364436787311675 1106 76-77 0.5317544251368282 1106 78-79 0.4552534364828915 1106 80-81 0.5836347360261307 1106 82-83 0.4928956930279149 1106 84-85 0.4721852435018512 1106 86-87 0.4690952850211545 1106 88-89 0.45549113492539917 1106 90-91 0.30348130559939435 1106 92-93 0.5461131383893552 1106 94-95 0.5330564509089939 1106 96-97 0.6250026543325475 1106 98-99 0.5750917819048134 1106 100-101 0.38130809756939854 1107 1 0.19924978692947093 1107 2 0.20338535983968242 1107 3 0.2032584534296511 1107 4 0.23269759920533772 1107 5 0.3025894962563598 1107 6 0.18543003319835805 1107 7 0.2563648025597516 1107 8 0.22892678199164607 1107 9 0.26752338981322765 1107 10-11 0.2836515327605156 1107 12-13 0.3066963501944002 1107 14-15 0.366970967450591 1107 16-17 0.3297158744338802 1107 18-19 0.3406305402605412 1107 20-21 0.4735875007451966 1107 22-23 0.3611552563691163 1107 24-25 0.20220085349131978 1107 26-27 0.28220024036524904 1107 28-29 0.20160183177271307 1107 30-31 0.3624503769214016 1107 32-33 0.34167930261048696 1107 34-35 0.5014428174318155 1107 36-37 0.4702789526612179 1107 38-39 0.5419674005505755 1107 40-41 0.30386513861231634 1107 42-43 0.4407003333730657 1107 44-45 0.533786554442699 1107 46-47 0.35047811999975664 1107 48-49 0.4434085708968425 1107 50-51 0.5313238975570869 1107 52-53 0.5554522765096266 1107 54-55 0.6323489733100089 1107 56-57 0.5357401744386792 1107 58-59 0.512936119760333 1107 60-61 0.31058371990567935 1107 62-63 0.47768697356980283 1107 64-65 0.4661745257992109 1107 66-67 0.3552025518444779 1107 68-69 0.5341952155566574 1107 70-71 0.43035547459825807 1107 72-73 0.4199221951623713 1107 74-75 0.47361333564326813 1107 76-77 0.35023850387829825 1107 78-79 0.21980795010181708 1107 80-81 0.34051917184480374 1107 82-83 0.4524203493006702 1107 84-85 0.3030638532089931 1107 86-87 0.384699073589303 1107 88-89 0.3443085593137134 1107 90-91 0.3818085597749352 1107 92-93 0.35311614456148277 1107 94-95 0.07118060649550628 1107 96-97 0.09447174461847752 1107 98-99 0.0737482614374656 1107 100-101 -0.16057033049526126 1108 1 0.17819257393645316 1108 2 0.20766382344553946 1108 3 0.23912104300621095 1108 4 0.14512129559805942 1108 5 0.22695355877091572 1108 6 0.1082667534282038 1108 7 0.1488991788748777 1108 8 0.20931414055107211 1108 9 0.2890964909764193 1108 10-11 0.3284100708473332 1108 12-13 0.2726777909216622 1108 14-15 0.30857642752461345 1108 16-17 0.31046966812772325 1108 18-19 0.33385392470709263 1108 20-21 0.3602868579701308 1108 22-23 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2316 94-95 -1.4273276873016094 2316 96-97 -1.3216740694695055 2316 98-99 -1.293505440329568 2316 100-101 -0.728593044233584 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7420.0 3 2318.0 4 2369.0 5 2572.0 6 2950.0 7 3528.0 8 4348.0 9 5291.0 10 6284.0 11 7644.0 12 9327.0 13 12597.0 14 11446.0 15 12217.0 16 14291.0 17 17593.0 18 15523.0 19 12688.0 20 13294.0 21 14180.0 22 15630.0 23 17611.0 24 19645.0 25 22819.0 26 25995.0 27 30436.0 28 35468.0 29 42181.0 30 50409.0 31 60938.0 32 75559.0 33 97257.0 34 130697.0 35 187180.0 36 291157.0 37 453204.0 38 413604.0 39 63611.0 40 10.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.11164482554234 18.63011549183231 14.619260611013333 23.63897907161201 2 15.390897616749575 20.004110613531704 39.63523284724272 24.969758922476 3 19.288978354049316 23.69015715714492 29.420998324339276 27.599866164466487 4 13.043299124038613 16.444865413809918 36.76162775693305 33.750207705218415 5 14.433363127014356 36.7277754081689 33.827011911133326 15.011849553683412 6 32.549840114352065 35.446514664819325 17.476336154880258 14.527309065948362 7 29.18781645490858 32.00612896992669 20.437019448980987 18.369035126183743 8 26.59272708183617 34.54826196117901 19.791042533664715 19.067968423320114 9 26.416316179605047 16.84740847739732 20.136646963365617 36.599628379632016 10-11 25.27846572456295 26.342438968322675 27.059696082632655 21.319399224481725 12-13 25.916584513439833 23.48769240202761 27.580948174569254 23.014774909963307 14-15 22.95142908082875 24.9873975171525 25.70368010599661 26.357493296022138 16-17 22.76089466017638 28.13565250759435 26.352694528675258 22.750758303554015 18-19 22.6658430515393 26.929816225218012 28.290975983925932 22.11336473931676 20-21 23.384963016112977 26.095017895137236 28.243271589056963 22.276747499692828 22-23 23.085557120263154 25.887072666423926 28.185148634272768 22.84222157904015 24-25 22.91038319457737 26.115692304628386 28.25208224467594 22.7218422561183 26-27 22.704255582539705 26.390251486712096 28.167063960586997 22.738428970161202 28-29 22.65693721379941 26.576251701503885 28.217321790451667 22.549489294245042 30-31 22.784151584020155 26.256488681594035 28.07484700866093 22.884512725724882 32-33 22.593841472355322 26.26918600125629 28.133932272668854 23.003040253719536 34-35 23.191272451004657 26.366393687109763 27.80801314218418 22.6343207197014 36-37 23.03921390641311 26.21097898606636 27.981890800163388 22.767916307357147 38-39 22.773560506375063 26.4546258904669 27.958596125051045 22.813217478106992 40-41 23.052528167431053 26.30722716249723 27.863135920685018 22.777108749386706 42-43 22.78988574302879 26.267350739792278 27.70094572436524 23.241817792813695 44-45 22.981397607420988 26.40107920198958 27.67565396554541 22.94186922504402 46-47 22.988971339097283 26.627430957405767 27.377198357047224 23.006399346449726 48-49 23.09579292902701 26.505799882713283 27.41275211651691 22.985655071742798 50-51 23.014531391941368 26.738074343774887 27.31353861508684 22.933855649196907 52-53 23.147655403778522 26.74826059556518 27.062089799767936 23.041994200888364 54-55 22.929411241486473 26.60322969460217 27.24678155995411 23.220577503957244 56-57 22.901519941217718 26.853852573800552 27.247534159034746 22.99709332594698 58-59 23.108774137559905 26.53240460258382 27.21368753573866 23.14513372411761 60-61 22.998564131492273 26.55327394117451 27.564072769716212 22.88408915761701 62-63 22.875130712407294 26.623335224773157 27.51622943853278 22.98530462428677 64-65 22.642505437683603 26.523913098735264 27.430186133997598 23.40339532958354 66-67 22.740408456921603 26.647337381513562 27.251296215810143 23.360957945754688 68-69 22.76516603711999 26.505129551331947 27.4936324650424 23.23607194650567 70-71 22.78100733403095 26.48377365514392 27.38607964153817 23.34913936928696 72-73 22.83672771400099 26.42449264715394 27.34699982048239 23.391779818362675 74-75 22.683031285554474 26.79406757599877 27.123702165933476 23.399198972513275 76-77 22.846198793916255 26.455146278783477 27.18503684625552 23.513618081044743 78-79 22.813494982238073 26.614783714654806 27.2181538756125 23.353567427494625 80-81 22.793837229852468 26.690704231998424 27.09709532347374 23.41836321467536 82-83 22.87368577016132 26.564862944805128 27.137284817092972 23.424166467940584 84-85 22.796164567084222 26.495765284866398 27.1394138475321 23.56865630051728 86-87 22.76392455340662 26.525249495314142 27.161595000178117 23.54923095110112 88-89 22.730580847032563 26.345661524202924 27.178791938206015 23.744965690558498 90-91 22.690628499881736 26.38486929764708 27.257050608942535 23.667451593528643 92-93 22.678138829451715 26.2189324278959 27.314472979079955 23.788455763572426 94-95 22.57116845438498 26.166526204976314 27.344565880315745 23.91773946032296 96-97 22.66029979269147 26.062107210718576 27.35317197028903 23.92442102630092 98-99 22.538040973015494 26.067113892890404 27.175654503556878 24.219190630537227 100-101 22.64715532902407 25.482817790658462 27.63564848368778 24.23437839662968 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1481.0 1 830.5 2 190.0 3 203.0 4 201.0 5 215.0 6 342.0 7 726.0 8 1267.5 9 1522.0 10 1469.5 11 1499.0 12 1624.0 13 1753.5 14 2004.5 15 2286.0 16 2486.5 17 2598.5 18 2657.5 19 2828.5 20 3089.0 21 3382.0 22 3933.5 23 4898.0 24 6014.5 25 7401.5 26 9425.5 27 11996.0 28 14527.5 29 17486.0 30 20996.0 31 24965.0 32 29464.5 33 33002.5 34 36657.5 35 40745.0 36 45201.0 37 49991.5 38 54233.0 39 57429.5 40 60185.0 41 64894.0 42 69656.0 43 73995.5 44 78006.5 45 81014.5 46 82489.5 47 85182.5 48 91480.5 49 93116.5 50 89417.0 51 86487.0 52 84810.5 53 83899.5 54 82366.0 55 76796.5 56 68297.5 57 61922.5 58 56769.0 59 49003.0 60 40571.5 61 33647.0 62 27064.5 63 21046.0 64 16262.0 65 12475.0 66 9356.5 67 7279.5 68 5564.0 69 4012.0 70 3007.0 71 2264.0 72 1779.0 73 1405.0 74 1044.0 75 827.0 76 592.5 77 332.5 78 201.5 79 139.5 80 86.0 81 56.0 82 38.5 83 30.5 84 30.0 85 24.5 86 17.0 87 13.0 88 8.0 89 4.5 90 5.0 91 6.5 92 6.0 93 5.0 94 4.0 95 2.5 96 2.5 97 3.0 98 2.5 99 2.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.28037919930031824 2 0.1074033223126219 3 0.11803059841513396 4 0.1190254923481351 5 0.12522096820364212 6 0.057613403211065395 7 0.06593433428707483 8 0.07050180188858002 9 0.06824067931357745 10-11 0.06394454642107257 12-13 0.06772062112132686 14-15 0.06371843416357233 16-17 0.06439677093607309 18-19 0.06878334873157807 20-21 0.07292120304383276 22-23 0.08097079941084191 24-25 0.08847772635985042 26-27 0.08906561822935109 28-29 0.08359370159784488 30-31 0.08006635038084088 32-33 0.07267247956058248 34-35 0.23809620714777024 36-37 0.36137260993691017 38-39 0.3342391390368794 40-41 0.31730333095011015 42-43 0.34054767102113653 44-45 0.3532325686669009 46-47 0.35911148736190757 48-49 0.36824642256491796 50-51 0.36297800696516197 52-53 0.3627971171591618 54-55 0.3499765521588972 56-57 0.3267548233136209 58-59 0.3129619756061052 60-61 0.2715834324835582 62-63 0.3392814423791351 64-65 0.3684273123709182 66-67 0.37279127894067315 68-69 0.3850917857486871 70-71 0.38647107051943863 72-73 0.36921870527216905 74-75 0.37288172384367324 76-77 0.3562850841431544 78-79 0.33555059013038085 80-81 0.33371908084462876 82-83 0.34692403668264377 84-85 0.3544083524059023 86-87 0.3478737081641448 88-89 0.3633397865771624 90-91 0.38610929090743823 92-93 0.3821975488526838 94-95 0.381519212080183 96-97 0.3859962347786881 98-99 0.37554984848217626 100-101 0.35363957073040136 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2211291.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.36081152818972 #Duplication Level Percentage of deduplicated Percentage of total 1 73.97990089400983 40.216074493734226 2 14.192388630326395 15.430195269359917 3 5.098099270942785 8.314104408591666 4 2.2774918363590078 4.952252178932109 5 1.233380941565828 3.352379445346057 6 0.7314111298018494 2.3856061546067187 7 0.49128213335689835 1.8694546821006925 8 0.3499483410171961 1.5218780648510768 9 0.26147504747513023 1.2792596197608013 >10 1.255520336288414 12.381795170165454 >50 0.0876774472630525 3.2642603084907447 >100 0.03950480251841766 3.810325648078658 >500 0.001418531055536646 0.5156104589352958 >1k 4.172150163342809E-4 0.4756480401508962 >5k 8.344300326685617E-5 0.23115605689576266 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5096 0.23045361284426158 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3880 0.17546311182019916 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2564 0.11595036564613159 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.35667354500154E-4 2 0.0 0.0 0.0 0.0 1.35667354500154E-4 3 0.0 0.0 0.0 0.0 1.35667354500154E-4 4 0.0 0.0 0.0 0.0 1.35667354500154E-4 5 0.0 0.0 0.0 0.0 1.35667354500154E-4 6 0.0 0.0 0.0 0.0 1.8088980600020532E-4 7 0.0 0.0 0.0 0.0 1.8088980600020532E-4 8 0.0 0.0 0.0 0.0 1.8088980600020532E-4 9 0.0 0.0 0.0 0.0 1.8088980600020532E-4 10-11 0.0 0.0 0.0 0.0 1.8088980600020532E-4 12-13 0.0 0.0 0.0 0.0 2.71334709000308E-4 14-15 0.0 0.0 0.0 2.2611225750025665E-5 3.165571605003593E-4 16-17 0.0 0.0 0.0 4.522245150005133E-5 3.6177961200041064E-4 18-19 0.0 0.0 0.0 4.522245150005133E-5 3.6177961200041064E-4 20-21 0.0 0.0 0.0 1.1305612875012833E-4 4.974469665005646E-4 22-23 0.0 0.0 0.0 1.8088980600020532E-4 5.878918695006672E-4 24-25 0.0 0.0 0.0 2.9394593475033366E-4 5.878918695006672E-4 26-27 0.0 0.0 0.0 4.296132892504876E-4 6.105030952506928E-4 28-29 0.0 0.0 0.0 0.001967176640252233 6.331143210007186E-4 30-31 0.0 0.0 0.0 0.004567467601505185 6.331143210007186E-4 32-33 0.0 0.0 0.0 0.010084606684511447 6.331143210007186E-4 34-35 0.0 0.0 0.0 0.020395325626523148 6.331143210007186E-4 36-37 0.0 0.0 0.0 0.039614867514044964 6.331143210007186E-4 38-39 0.0 0.0 0.0 0.06975563143882918 6.331143210007186E-4 40-41 0.0 0.0 0.0 0.1043960292878685 6.331143210007186E-4 42-43 0.0 0.0 0.0 0.1467016326661665 6.331143210007186E-4 44-45 0.0 0.0 0.0 0.20087812956322798 6.557255467507443E-4 46-47 0.0 0.0 0.0 0.26333033508479886 7.009479982507956E-4 48-49 0.0 0.0 0.0 0.3340808604566292 7.235592240008213E-4 50-51 0.0 0.0 0.0 0.41572999663997184 7.687816755008725E-4 52-53 0.0 0.0 0.0 0.49552501231181245 9.722827072511035E-4 54-55 0.0 0.0 0.0 0.5885928174989181 9.948939330011292E-4 56-57 0.0 0.0 0.0 0.6972397572277913 9.948939330011292E-4 58-59 0.0 0.0 0.0 0.8240209000081853 9.948939330011292E-4 60-61 0.0 0.0 0.0 0.9609997056018407 9.948939330011292E-4 62-63 0.0 0.0 0.0 1.104106153373753 9.948939330011292E-4 64-65 0.0 0.0 0.0 1.2552848087384247 0.0010175051587511547 66-67 0.0 0.0 0.0 1.4377121780896318 0.0010401163845011805 68-69 0.0 0.0 0.0 1.6448536171856167 0.0010401163845011805 70-71 0.0 0.0 0.0 1.8735435544213765 0.0010401163845011805 72-73 0.0 0.0 0.0 2.1098760859606447 0.0010853388360012317 74-75 0.0 0.0 0.0 2.3596622968211785 0.0011305612875012832 76-77 0.0 0.0 0.0 2.6392501032202453 0.0011305612875012832 78-79 0.0 0.0 0.0 2.956259488235605 0.0013114510935014884 80-81 4.522245150005133E-5 0.0 0.0 3.2963775459674913 0.0013114510935014884 82-83 4.522245150005133E-5 0.0 0.0 3.657501432421151 0.0013566735450015399 84-85 4.522245150005133E-5 0.0 0.0 4.031739829809826 0.0013566735450015399 86-87 4.522245150005133E-5 0.0 0.0 4.428634675400026 0.001469729673751668 88-89 4.522245150005133E-5 0.0 0.0 4.862363207737019 0.0015827858025017965 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 8815 0.0 30.659502 1 GGTATCA 5890 0.0 27.821398 1 ATCAACG 10440 0.0 25.252907 3 TCAACGC 10510 0.0 25.129831 4 CAACGCA 10550 0.0 24.944662 5 TATCAAC 10600 0.0 24.916462 2 AACGCAG 10770 0.0 24.333288 6 GTACATG 7500 0.0 22.7357 1 TACATGG 7515 0.0 22.147015 2 ACGCAGA 11835 0.0 21.983427 7 CGCAGAG 11940 0.0 21.750416 8 GAGTACT 5200 0.0 21.235073 12-13 ACATGGG 7760 0.0 20.714527 3 AGAGTAC 9360 0.0 20.37916 10-11 AGTACTT 5760 0.0 19.993322 12-13 GAGTACA 6350 0.0 19.821993 1 GCAGAGT 12785 0.0 19.645653 9 GTGTAGC 1370 0.0 19.415234 1 GTACTTT 5700 0.0 19.413918 14-15 AGTACAT 6065 0.0 18.45093 2 >>END_MODULE