FastQCFastQC Report
Fri 27 May 2016
SRR939306_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939306_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2211291
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50740.22945871891126043No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42320.1913814147482172No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27560.12463307633414145No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA59150.030.5032331
GTATCAA86000.029.1171421
GTACATG78950.023.9386581
ATCAACG103950.023.7379683
TCAACGC105350.023.5126024
TACATGG79550.023.443212
CAACGCA105900.023.3456785
TATCAAC107250.023.317292
AACGCAG108950.022.7792386
ACATGGG80300.022.1605453
GAGTACT58800.020.54066312-13
ACGCAGA122700.020.1105277
CGCAGAG124700.019.6738268
AGAGTAC103050.019.22660810-11
AGTACTT62850.019.02826912-13
CATGGGG48950.019.0006354
GTACTTT62700.018.99810214-15
GCAGAGT135950.017.8371759
GTATAGC8600.017.713851
GGACCGA4255.820766E-1116.7481586
Produced by FastQC (version 0.11.5)