FastQCFastQC Report
Fri 27 May 2016
SRR939305_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939305_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1627864
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61980.38074433736479213No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51110.31396971737196716No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT31250.19196935370522353No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27910.17145166918120924No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT20800.1277748018261968No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA133000.026.6513711
GAGTACT57950.024.65412312-13
GGTATCA97350.024.3716341
GTACTTT61500.023.2738214-15
GTACATG99050.023.138931
TACATGG98800.022.8902742
TCAACGC155100.022.8348624
ATCAACG154950.022.8263663
CAACGCA155600.022.670075
TATCAAC156200.022.6133442
AACGCAG158500.022.2742316
ATACCGT1754.1341045E-621.6634526
AGTACTT59550.021.5646912-13
ACATGGG101500.021.4872743
ACGCAGA172650.020.3663447
CGCAGAG174100.020.088468
CATGGGG47800.020.0360934
ACTTTTT72100.019.75901816-17
ACCGTAA1456.2440394E-419.6097628
AGAGTAC129150.019.42497410-11
Produced by FastQC (version 0.11.5)