##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939305_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1627864 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.825379147152344 33.0 31.0 34.0 30.0 34.0 2 31.902439024390244 33.0 31.0 34.0 30.0 34.0 3 31.93575507536256 34.0 31.0 34.0 30.0 34.0 4 35.42791289690048 37.0 35.0 37.0 33.0 37.0 5 35.46426114220844 37.0 35.0 37.0 33.0 37.0 6 35.554002668527595 37.0 35.0 37.0 33.0 37.0 7 35.488345463748814 37.0 35.0 37.0 33.0 37.0 8 35.47692006211821 37.0 35.0 37.0 33.0 37.0 9 37.082857658870765 39.0 37.0 39.0 33.0 39.0 10-11 36.979149363828924 39.0 37.0 39.0 33.0 39.0 12-13 36.87687853530762 39.0 37.0 39.0 33.0 39.0 14-15 38.0695884299917 40.0 38.0 41.0 33.0 41.0 16-17 37.98718781175823 40.0 38.0 41.0 33.0 41.0 18-19 37.937438569806815 40.0 38.0 41.0 33.0 41.0 20-21 37.914523264842764 40.0 38.0 41.0 33.0 41.0 22-23 37.908856636672354 40.0 38.0 41.0 33.0 41.0 24-25 37.81730169105036 40.0 38.0 41.0 32.0 41.0 26-27 37.72770729004388 40.0 38.0 41.0 32.0 41.0 28-29 37.58477704525686 40.0 37.5 41.0 32.0 41.0 30-31 37.39724080144288 40.0 37.0 41.0 31.5 41.0 32-33 37.16114767572721 40.0 36.5 41.0 31.0 41.0 34-35 36.864923605411754 40.0 36.0 41.0 30.0 41.0 36-37 36.621844330976046 39.5 36.0 41.0 30.0 41.0 38-39 36.028045647547955 38.5 35.5 40.5 28.5 41.0 40-41 35.99794055277345 39.0 35.0 40.0 29.0 41.0 42-43 36.17301568189971 39.0 35.0 41.0 29.5 41.0 44-45 36.04456146213688 39.0 35.0 41.0 28.5 41.0 46-47 35.730465812868886 39.0 35.0 41.0 27.0 41.0 48-49 35.43989516323231 39.0 35.0 41.0 26.0 41.0 50-51 34.28268854154892 38.0 34.0 40.0 24.5 40.5 52-53 34.37022625968754 38.0 34.0 39.5 24.0 40.5 54-55 34.84893731908808 39.0 35.0 40.0 24.5 41.0 56-57 34.73925800926859 38.0 34.5 40.0 24.5 41.0 58-59 34.50885147653612 38.0 34.0 40.0 24.0 41.0 60-61 34.17907024174009 38.0 34.0 40.0 23.0 41.0 62-63 33.784836755404626 37.0 33.5 40.0 22.5 41.0 64-65 33.37193309760521 36.5 33.0 39.5 21.5 41.0 66-67 32.984590850341306 36.0 33.0 39.0 20.0 41.0 68-69 32.57194673510809 35.5 32.0 39.0 19.5 40.5 70-71 32.14160396691616 35.0 32.0 38.5 18.0 40.0 72-73 31.702900856582616 35.0 31.5 37.5 14.5 39.5 74-75 31.2566141889003 35.0 31.0 37.0 11.5 39.0 76-77 30.804550933001774 35.0 31.0 36.0 7.5 39.0 78-79 30.426132342750993 35.0 30.5 36.0 7.0 38.5 80-81 30.03478453974042 34.0 30.0 35.5 2.0 37.0 82-83 29.620637841981885 34.0 30.0 35.0 2.0 37.0 84-85 29.214911380803308 34.0 29.0 35.0 2.0 36.0 86-87 28.806861015416523 34.0 29.0 35.0 2.0 36.0 88-89 28.38389908493584 34.0 29.0 35.0 2.0 35.5 90-91 27.969633212602524 34.0 27.5 35.0 2.0 35.0 92-93 27.51954432311299 33.5 27.0 35.0 2.0 35.0 94-95 27.036695018748496 33.0 25.5 35.0 2.0 35.0 96-97 26.41558324282618 33.0 25.0 35.0 2.0 35.0 98-99 25.60477011593106 33.0 22.0 35.0 2.0 35.0 100-101 22.929083756382596 29.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4444593275251698 1101 2 0.48856150302584567 1101 3 0.4907327234932488 1101 4 0.3361601804167904 1101 5 0.3270640517048875 1101 6 0.36744286094982925 1101 7 0.3484461739075613 1101 8 0.3008909562421138 1101 9 0.3092017573476298 1101 10-11 0.46944089450690996 1101 12-13 0.5350024549591907 1101 14-15 0.6281988487008761 1101 16-17 0.6310592564954831 1101 18-19 0.6928990191006221 1101 20-21 0.6998657373283095 1101 22-23 0.7596068467619759 1101 24-25 0.7860956989810219 1101 26-27 0.7741610540512696 1101 28-29 0.7771764567102508 1101 30-31 0.895483323986106 1101 32-33 0.8908342933776936 1101 34-35 0.7796011233334426 1101 36-37 0.7157458551752569 1101 38-39 1.2185444813184745 1101 40-41 0.9659979857881424 1101 42-43 1.0311359951274213 1101 44-45 1.0636060938360004 1101 46-47 1.3018210014570286 1101 48-49 0.8768896805582571 1101 50-51 0.7843787396596014 1101 52-53 0.8178695635327244 1101 54-55 0.8854924649541758 1101 56-57 0.8442127015750742 1101 58-59 0.9724633673614562 1101 60-61 0.8156980089175292 1101 62-63 0.9937610049245436 1101 64-65 0.8677130100213617 1101 66-67 0.930941192644319 1101 68-69 0.9768118769456251 1101 70-71 0.8983143837851486 1101 72-73 0.8857376540547044 1101 74-75 0.8274857107392641 1101 76-77 0.8524959414696234 1101 78-79 0.8534709513358472 1101 80-81 0.7795288290955789 1101 82-83 0.7541904434841236 1101 84-85 0.7541897921503029 1101 86-87 0.707031212888932 1101 88-89 0.6952524461401239 1101 90-91 0.9424779822413853 1101 92-93 0.9379225895228096 1101 94-95 0.9090197596791292 1101 96-97 0.925294725247916 1101 98-99 1.1497432183921283 1101 100-101 0.639226069278731 1102 1 0.277763984880135 1102 2 0.3344337604740808 1102 3 0.4022099782713262 1102 4 0.33617381325896645 1102 5 0.40499595894890916 1102 6 0.42370513050779124 1102 7 0.3872132760730267 1102 8 0.4562329619434635 1102 9 0.3426144432243774 1102 10-11 0.4711567522289215 1102 12-13 0.5190503842714946 1102 14-15 0.6550452056293707 1102 16-17 0.6150465431650147 1102 18-19 0.6350905972008292 1102 20-21 0.6598521702998568 1102 22-23 0.7961038456563116 1102 24-25 0.6989729056210621 1102 26-27 0.5357517904879998 1102 28-29 0.4879061146293324 1102 30-31 0.5469021267781145 1102 32-33 0.6895308056265606 1102 34-35 0.5485883817970816 1102 36-37 0.386510836258509 1102 38-39 0.972425610456277 1102 40-41 0.7865841415899055 1102 42-43 0.7010103755382531 1102 44-45 0.7768907344178686 1102 46-47 0.7163127596987877 1102 48-49 0.6932275606795031 1102 50-51 0.6706441682761621 1102 52-53 0.7355582232644977 1102 54-55 0.7140740476425407 1102 56-57 0.8051186462464273 1102 58-59 0.6506636535571246 1102 60-61 0.6738673250565554 1102 62-63 0.6456090071960574 1102 64-65 0.6504285405551684 1102 66-67 0.6613592602030494 1102 68-69 0.5168637569615768 1102 70-71 0.4985860534228337 1102 72-73 0.5248779670271553 1102 74-75 0.5514899961526858 1102 76-77 0.37399234804046344 1102 78-79 0.6610353923705183 1102 80-81 0.5144450294250227 1102 82-83 0.3694634105923065 1102 84-85 0.5867575708808133 1102 86-87 0.4915514496678952 1102 88-89 0.7526100440521333 1102 90-91 0.6521116066594033 1102 92-93 0.6637224142702713 1102 94-95 0.6553546405750978 1102 96-97 0.6857066909345235 1102 98-99 0.7321805449259635 1102 100-101 0.669646111249083 1103 1 0.31278703395528495 1103 2 0.45002549084525967 1103 3 0.39988857767922426 1103 4 0.445096842817307 1103 5 0.38832558133991313 1103 6 0.32427936447308525 1103 7 0.417960494927911 1103 8 0.3887460395791251 1103 9 0.4540485437606776 1103 10-11 0.5230233562218913 1103 12-13 0.499844883280538 1103 14-15 0.6762518666334145 1103 16-17 0.6429074735394877 1103 18-19 0.6592433340895241 1103 20-21 0.7532160433141613 1103 22-23 0.7392240334574822 1103 24-25 0.6786816103922391 1103 26-27 0.5797828084682024 1103 28-29 0.5876569317238562 1103 30-31 0.6535113150713201 1103 32-33 0.6340783318024492 1103 34-35 0.539860107990016 1103 36-37 0.5301407975822485 1103 38-39 1.0281124234165375 1103 40-41 0.8716609306077245 1103 42-43 0.7600631506359008 1103 44-45 0.7414691026478053 1103 46-47 0.675482773085669 1103 48-49 0.6209253470336833 1103 50-51 0.5821945450055281 1103 52-53 0.7231224431831933 1103 54-55 0.7436172718741645 1103 56-57 0.727957846991913 1103 58-59 0.7514892695048303 1103 60-61 0.9454629477939136 1103 62-63 0.6229172820476876 1103 64-65 0.605303481798579 1103 66-67 0.6846694757400726 1103 68-69 0.7046030783814032 1103 70-71 0.6544566267496776 1103 72-73 0.6458168904140642 1103 74-75 0.6725017639631474 1103 76-77 0.6391006938123276 1103 78-79 0.7470268599519976 1103 80-81 0.6352286430032272 1103 82-83 0.5457867738605735 1103 84-85 0.4831721133765967 1103 86-87 0.5126529673058648 1103 88-89 0.44436611694054307 1103 90-91 0.3970214307607378 1103 92-93 0.23356578501539715 1103 94-95 0.21471185038627993 1103 96-97 0.41325342819155964 1103 98-99 0.3861228026632766 1103 100-101 0.25888602779410874 1104 1 0.32826045846844565 1104 2 0.4096253215797283 1104 3 0.3438034019863956 1104 4 0.30488856104874884 1104 5 0.19591777915702835 1104 6 0.12099571962197331 1104 7 0.20780376462698058 1104 8 0.47061892542336636 1104 9 0.605324289553657 1104 10-11 0.5019609754862344 1104 12-13 0.5380221117437642 1104 14-15 0.4850303869439472 1104 16-17 0.4504876950218417 1104 18-19 0.44031061181539854 1104 20-21 0.5652241818989836 1104 22-23 0.4580922165942667 1104 24-25 0.6440573612418916 1104 26-27 0.5439762289360246 1104 28-29 0.5783599691884973 1104 30-31 0.7166645243298362 1104 32-33 0.7079936085489322 1104 34-35 0.7155176753036656 1104 36-37 0.5100924131898097 1104 38-39 1.0149364591219836 1104 40-41 0.6964085626201992 1104 42-43 0.585858711940098 1104 44-45 0.6401825781535351 1104 46-47 0.6692617325284758 1104 48-49 0.6509772977124726 1104 50-51 0.6627340655795351 1104 52-53 0.5761049497502171 1104 54-55 0.6106160822249151 1104 56-57 0.523779303793269 1104 58-59 0.48069169883780205 1104 60-61 0.48814660488749695 1104 62-63 0.6107320267787699 1104 64-65 0.47513669137645564 1104 66-67 0.5512145160737063 1104 68-69 0.4558492825486411 1104 70-71 0.5296379351509444 1104 72-73 0.5013177540408726 1104 74-75 0.5108950987336414 1104 76-77 0.5191004808997093 1104 78-79 0.4876839881333801 1104 80-81 0.4190604580034538 1104 82-83 0.3104438115540944 1104 84-85 0.5154516623212189 1104 86-87 0.37214128505665656 1104 88-89 0.30961571706403035 1104 90-91 0.4059717449399187 1104 92-93 0.338765244217349 1104 94-95 0.19357228882659072 1104 96-97 0.15749879933817112 1104 98-99 0.39459007134037094 1104 100-101 -0.25633190157745034 1105 1 0.11345577225690562 1105 2 0.2573478637239752 1105 3 0.36439949789531667 1105 4 0.3074352288977238 1105 5 0.23761673431639707 1105 6 0.2592495836389048 1105 7 0.26093809375242216 1105 8 0.29466923678509716 1105 9 0.3888720870179583 1105 10-11 0.38387205158512216 1105 12-13 0.47910798178531167 1105 14-15 0.43411352226261357 1105 16-17 0.42434868765256084 1105 18-19 0.5003085213630669 1105 20-21 0.5305258860031685 1105 22-23 0.4778114362900965 1105 24-25 0.5030882652382971 1105 26-27 0.3893840663331005 1105 28-29 0.40474190596687265 1105 30-31 0.36326370536942676 1105 32-33 0.5333682300292679 1105 34-35 0.4664951116980163 1105 36-37 0.45903932640990774 1105 38-39 0.8761075065285624 1105 40-41 0.5531020581863331 1105 42-43 0.335010429516565 1105 44-45 0.2606204765121518 1105 46-47 0.4716456620905163 1105 48-49 0.48269931210317907 1105 50-51 0.295610155302775 1105 52-53 0.1922231511216026 1105 54-55 0.38080597497357616 1105 56-57 0.31530594656538824 1105 58-59 0.372451247128744 1105 60-61 0.5924231220396976 1105 62-63 0.5945811406711456 1105 64-65 0.5545665976102399 1105 66-67 0.5518607142991385 1105 68-69 0.6042763242630187 1105 70-71 0.5830873314257445 1105 72-73 0.6856528124257473 1105 74-75 0.648247669368871 1105 76-77 0.7659372795433157 1105 78-79 0.7603875641348132 1105 80-81 0.6537814147452252 1105 82-83 0.5703063129088157 1105 84-85 0.7935199472892833 1105 86-87 0.6493389051966219 1105 88-89 0.5864935579694688 1105 90-91 0.4828787579362803 1105 92-93 0.7204452139571949 1105 94-95 0.7484468766797754 1105 96-97 0.8101727149053168 1105 98-99 0.5939093463817819 1105 100-101 0.41807222312488435 1106 1 0.11658335130013242 1106 2 0.21092569543720785 1106 3 0.21117902247728537 1106 4 0.05209338243489725 1106 5 0.20757237985472443 1106 6 0.14438494402399016 1106 7 0.1938034895478964 1106 8 0.12201370142146573 1106 9 -0.0011963351259396404 1106 10-11 0.2025755271698344 1106 12-13 0.2392162050455866 1106 14-15 0.3116980538981906 1106 16-17 0.3087481949155588 1106 18-19 0.24278757394201023 1106 20-21 0.17268132582785967 1106 22-23 0.35358967211272585 1106 24-25 0.2624632779429419 1106 26-27 0.16128870627326108 1106 28-29 0.2806843874587486 1106 30-31 0.1765354872316678 1106 32-33 0.17309982658662193 1106 34-35 0.0575522602468439 1106 36-37 0.018619239652416297 1106 38-39 0.4343714216737169 1106 40-41 0.45605258527670856 1106 42-43 0.3426437876493651 1106 44-45 0.10487492720388758 1106 46-47 -0.0868318943892632 1106 48-49 -0.1171691693968242 1106 50-51 -0.140372452544419 1106 52-53 -0.10300091818681523 1106 54-55 0.09420073781339511 1106 56-57 -0.02561114596103664 1106 58-59 0.003907748452064652 1106 60-61 0.012709291659945166 1106 62-63 -0.19694455523856647 1106 64-65 -0.008343559195587602 1106 66-67 -0.17926424657055406 1106 68-69 -0.09833066918748301 1106 70-71 -0.11331003739115886 1106 72-73 -0.15494717212540365 1106 74-75 -0.127581588313884 1106 76-77 0.04349436914257865 1106 78-79 0.1530459046843866 1106 80-81 0.15757715655428584 1106 82-83 0.044813067715239185 1106 84-85 0.04244442273984106 1106 86-87 0.0658569783184717 1106 88-89 0.11457651829438475 1106 90-91 0.16660542712354598 1106 92-93 0.31600244991398796 1106 94-95 0.409141139871263 1106 96-97 0.41798556428309297 1106 98-99 0.5052256290632151 1106 100-101 0.2704151632036762 1107 1 0.20764097160594375 1107 2 0.2085116253896011 1107 3 0.376638266130751 1107 4 0.285979600663417 1107 5 0.3062935667919007 1107 6 0.21357043787629948 1107 7 0.2096912674938025 1107 8 0.07233088275607003 1107 9 0.22649097645670935 1107 10-11 0.352060665235868 1107 12-13 0.3915125203102505 1107 14-15 0.35604795825880586 1107 16-17 0.4996459635885131 1107 18-19 0.3800566717502605 1107 20-21 0.37465037203352125 1107 22-23 0.2899151796998609 1107 24-25 0.4079641528590443 1107 26-27 0.49629194598474413 1107 28-29 0.29593586278843986 1107 30-31 0.567509689816454 1107 32-33 0.4081414772487264 1107 34-35 0.3981592308644011 1107 36-37 0.28705494398897713 1107 38-39 0.8187115875003883 1107 40-41 0.40641768330223016 1107 42-43 0.16880009305909738 1107 44-45 0.4008006505131263 1107 46-47 0.4004583488412905 1107 48-49 0.39052213847664774 1107 50-51 0.435837783391932 1107 52-53 0.4323195028592792 1107 54-55 0.48290384444452883 1107 56-57 0.34091739647918473 1107 58-59 0.41852777103304817 1107 60-61 0.4424205499483662 1107 62-63 0.3907676264646298 1107 64-65 0.33814106284453516 1107 66-67 0.25305488247084185 1107 68-69 0.4826855914482735 1107 70-71 0.5336617053408332 1107 72-73 0.4677067529693666 1107 74-75 0.4930739973755145 1107 76-77 0.498644152450499 1107 78-79 0.6098586105612611 1107 80-81 0.4075453414234751 1107 82-83 0.33510072305649174 1107 84-85 0.40199381185053085 1107 86-87 0.49455383832025746 1107 88-89 0.3904294159932178 1107 90-91 0.5008160044905736 1107 92-93 0.5563599433472675 1107 94-95 0.48461729986257396 1107 96-97 0.6361165029458498 1107 98-99 0.7373748187473517 1107 100-101 0.5051369166053696 1108 1 0.18036506601169222 1108 2 0.07517600312778328 1108 3 0.15903647988048775 1108 4 0.15773795183747552 1108 5 0.24622396121488777 1108 6 0.14097042554561767 1108 7 0.3223933069660063 1108 8 0.17285434593441096 1108 9 0.27933052146202897 1108 10-11 0.44747520310674105 1108 12-13 0.4164166160524374 1108 14-15 0.34936502543258285 1108 16-17 0.3551964859626082 1108 18-19 0.5069633584118236 1108 20-21 0.5943766161861319 1108 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-1.3624152925553936 2316 94-95 -1.3641624020022896 2316 96-97 -1.3177310858327616 2316 98-99 -1.356210981330694 2316 100-101 -0.7627526620197607 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6711.0 3 1615.0 4 1709.0 5 1798.0 6 2229.0 7 2610.0 8 3288.0 9 3994.0 10 4723.0 11 5559.0 12 6809.0 13 8580.0 14 8883.0 15 9680.0 16 12289.0 17 15881.0 18 14441.0 19 9942.0 20 10003.0 21 10629.0 22 11462.0 23 12830.0 24 14191.0 25 15901.0 26 18836.0 27 21837.0 28 26153.0 29 30442.0 30 36743.0 31 44791.0 32 56340.0 33 73287.0 34 99838.0 35 146470.0 36 228224.0 37 338349.0 38 275974.0 39 34822.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.6652587065167 19.32585487988802 16.325704555087878 21.683181858507396 2 15.2362777250234 20.50585532554921 39.4633372124724 24.794529736954985 3 19.009536492963328 25.3009386331355 29.96727215437598 25.722252719525194 4 13.086603655702842 17.322085332561855 37.34566058408763 32.245650427647675 5 14.043886877153927 37.54923483616297 33.27308388914266 15.133794397540443 6 30.858346109543938 36.01150996667423 17.6961125265902 15.434031397191633 7 28.012208076102418 33.40558454283376 20.522336879670366 18.059870501393462 8 27.123960465229295 33.318139008997875 19.792299122727695 19.76560140304514 9 27.23736581464735 16.682169111992863 20.158777029313153 35.92168804404663 10-11 25.48559007082252 26.746266655348844 26.240747370143332 21.527395903685306 12-13 26.655719153228286 24.14437187104075 26.787027811604364 22.412881164126595 14-15 23.667548754742608 25.021990689480393 25.942739320213963 25.367721235563035 16-17 22.313812491063644 28.374821080726225 26.431592493000817 22.879773935209315 18-19 22.333992575072052 26.899062195305746 28.901147572749835 21.865797656872367 20-21 23.89653365723482 25.483275601462108 28.652421523573768 21.9677692177293 22-23 23.309874495112965 25.555898778917935 28.683121697158846 22.45110502881025 24-25 23.017790812542508 25.78858445942827 28.776185361740364 22.417439366288857 26-27 22.57515050408021 25.85639483113944 29.042656822390107 22.525797842390244 28-29 22.451698457458182 26.25296332021088 28.86930007953467 22.426038142796266 30-31 22.63576112895619 25.88817682903006 28.69521585401908 22.780846187994673 32-33 22.549707048166063 25.962089020559702 28.554458150639057 22.93374578063518 34-35 23.19916406850816 25.874607193880934 28.396179743764804 22.5300489938461 36-37 23.194329021548842 25.54649762274267 28.62500497608547 22.634168379623016 38-39 22.811660040825505 25.918674136638035 28.543580779991125 22.726085042545336 40-41 22.885406863638703 25.825305067916766 28.682568662451345 22.606719405993182 42-43 22.720581905960458 25.808347558983357 28.217323710181162 23.253746824875023 44-45 22.86683722836014 25.993781039266217 28.102507330943986 23.036874401429657 46-47 23.213383750727026 26.276687817904964 27.560912017945384 22.94901641342263 48-49 23.162659395419137 26.182261768614108 27.549478078558852 23.105600757407903 50-51 23.10047136451325 26.52872558784795 27.52545356028936 22.845349487349438 52-53 23.2867775939961 26.49714864097563 27.20915520774187 23.006918557286397 54-55 22.964045113686677 26.53728851995205 27.29976597776403 23.19890038859724 56-57 23.155626724830277 26.617778227383365 27.156523605732634 23.07007144205372 58-59 23.25363015612308 26.20176161949093 27.33498463316658 23.20962359121941 60-61 23.075099664655752 26.324014858841927 27.67291313014988 22.92797234635244 62-63 22.957984303129685 26.42149127056766 27.735858838021805 22.884665588280857 64-65 22.84372696305974 26.528865970792282 27.48056835535571 23.146838710792267 66-67 22.82647472352213 26.466823272653656 27.286535876390722 23.420166127433493 68-69 22.93434534542135 26.34899108460643 27.492442933888555 23.224220636083672 70-71 23.120618593289727 26.372307117804 27.176410498556443 23.330663790349835 72-73 22.98833229490789 26.267596528048443 27.343146068039857 23.40092510900381 74-75 22.727809988016702 26.910515337419525 26.926933352425962 23.43474132213781 76-77 22.94427015713009 26.39127145544031 27.115955640244653 23.548502747184937 78-79 23.036397093415147 26.46673484277079 27.1665424997802 23.330325564033867 80-81 23.064030917263988 26.552618947435963 27.003680802940693 23.379669332359356 82-83 23.043085365180175 26.475574490561243 27.165243918805725 23.316096225452856 84-85 22.85360095470931 26.569945168388458 27.091357634650915 23.485096242251316 86-87 22.98494559694037 26.59619434662336 27.008143335540353 23.410716720895923 88-89 22.838576700443085 26.440516890265727 26.955463263517764 23.76544314577342 90-91 22.790264755614356 26.440070993229348 27.084299676358025 23.685364574798268 92-93 22.924691001387593 26.260252630747278 27.2005194830847 23.614536884780428 94-95 22.730079800851247 26.280333474168653 27.223364301672337 23.766222423307763 96-97 22.632256766336376 26.161593373883463 27.42766109850756 23.778488761272598 98-99 22.666833725159567 26.24884967266205 26.974813463350927 24.10950313882746 100-101 22.82770549840881 25.638249489196767 27.522621286070176 24.011423726324246 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1750.0 1 1014.5 2 275.5 3 295.5 4 288.5 5 295.0 6 460.0 7 881.5 8 1487.0 9 1897.0 10 1871.0 11 1709.0 12 1719.5 13 1867.0 14 2053.0 15 2197.0 16 2290.0 17 2410.0 18 2520.0 19 2576.0 20 2714.0 21 3018.5 22 3485.5 23 4128.5 24 4953.5 25 5984.0 26 7606.0 27 9433.5 28 10933.0 29 13137.5 30 15930.5 31 18920.5 32 22280.0 33 25116.0 34 27758.5 35 30155.0 36 33030.5 37 36595.5 38 39436.0 39 41420.0 40 43235.0 41 45506.5 42 47829.0 43 50169.0 44 52748.0 45 55304.0 46 56973.0 47 61499.5 48 70582.0 49 70979.5 50 63870.0 51 60086.5 52 58496.0 53 58737.0 54 58756.5 55 56020.0 56 51892.0 57 47435.0 58 43290.5 59 38461.5 60 32391.0 61 27374.5 62 22560.0 63 16705.5 64 11939.0 65 9277.0 66 7122.5 67 5617.0 68 4435.0 69 3442.5 70 2684.0 71 1865.5 72 1408.5 73 1130.5 74 860.5 75 667.5 76 486.0 77 311.0 78 207.5 79 140.0 80 89.0 81 69.5 82 46.5 83 34.5 84 28.5 85 20.5 86 12.5 87 9.5 88 8.5 89 6.0 90 5.0 91 3.5 92 4.0 93 3.5 94 2.5 95 2.5 96 1.5 97 1.5 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.28945907029088425 2 0.1801747566135746 3 0.18109620951135966 4 0.20142960345581692 5 0.2153742573089644 6 0.11026719676828041 7 0.126239046996555 8 0.13864794602006064 9 0.1421494670316439 10-11 0.12961770762176691 12-13 0.11819169168923202 14-15 0.13367210037202124 16-17 0.10943788916027383 18-19 0.138770806406432 20-21 0.1645407724478212 22-23 0.16881017087422537 24-25 0.19344367834167966 26-27 0.17354029574952207 28-29 0.17117523331187373 30-31 0.16036351931119552 32-33 0.17000805964134594 34-35 0.3514728503118197 36-37 0.4677602060122959 38-39 0.43391216956699086 40-41 0.4221790026685276 42-43 0.44589105723819683 44-45 0.45065189721008636 46-47 0.45406127293189114 48-49 0.46693089840428925 50-51 0.4583921015514809 52-53 0.46588658512013287 54-55 0.44791825361332394 56-57 0.4109679924121425 58-59 0.40021770860465 60-61 0.356264405380302 62-63 0.463245086813149 64-65 0.461002884761872 66-67 0.4706781401886153 68-69 0.4826570278598212 70-71 0.47525468958094774 72-73 0.46825164755778126 74-75 0.47255176108077823 76-77 0.44819468948265945 78-79 0.43378930918061953 80-81 0.4313628165497855 82-83 0.4442938722153693 84-85 0.44635178368708933 86-87 0.4469046554257604 88-89 0.454829150346712 90-91 0.47359607436493467 92-93 0.4786640653027525 94-95 0.4772204557628893 96-97 0.47482467822864804 98-99 0.47135387231365766 100-101 0.45317053513069894 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1627864.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.590345889875714 #Duplication Level Percentage of deduplicated Percentage of total 1 75.53711999875316 42.746717482544895 2 13.806210423093567 15.625964465425449 3 4.643703756388088 7.8836640535235105 4 2.060326512588787 4.663783599739233 5 1.1089136470653878 3.1376903424716924 6 0.6730562773645524 2.285309252364729 7 0.4214352709016307 1.6694417425361727 8 0.2993683966427917 1.3553088891610472 9 0.21694221450074005 1.1049151461041242 >10 1.1326537602485107 11.51328848028449 >50 0.06951458184290685 2.655763929278921 >100 0.027495147722788695 2.970544421941232 >500 0.002173341924733472 0.8375630662644972 >1k 8.693367698933889E-4 0.8545972049836799 >5k 2.1733419247334723E-4 0.6954479233762996 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6198 0.38074433736479213 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5111 0.31396971737196716 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3125 0.19196935370522353 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2791 0.17145166918120924 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2080 0.1277748018261968 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 6.143019318567153E-5 0.0 20-21 0.0 0.0 0.0 1.5357548296417883E-4 0.0 22-23 0.0 0.0 0.0 1.842905795570146E-4 0.0 24-25 0.0 0.0 0.0 1.842905795570146E-4 0.0 26-27 0.0 0.0 0.0 4.300113522997007E-4 0.0 28-29 0.0 0.0 0.0 9.828830909707445E-4 0.0 30-31 0.0 0.0 0.0 0.002948649272912234 0.0 32-33 0.0 0.0 0.0 0.006204449511752825 0.0 34-35 0.0 0.0 0.0 0.011856027284834605 0.0 36-37 0.0 0.0 0.0 0.021961294063877573 0.0 38-39 0.0 0.0 0.0 0.03860887641719456 0.0 40-41 0.0 0.0 0.0 0.059249421327580196 0.0 42-43 0.0 0.0 0.0 0.08188644751650015 0.0 44-45 0.0 0.0 0.0 0.11217153275703622 0.0 46-47 0.0 0.0 0.0 0.14902964866843915 0.0 48-49 0.0 0.0 0.0 0.19344367834167964 0.0 50-51 0.0 0.0 0.0 0.2446764594585297 0.0 52-53 0.0 0.0 0.0 0.2968306934731648 0.0 54-55 0.0 0.0 0.0 0.3593666301361784 0.0 56-57 0.0 0.0 0.0 0.43142424674297114 0.0 58-59 0.0 0.0 0.0 0.5151843151516342 0.0 60-61 0.0 0.0 0.0 0.6149162337885721 0.0 62-63 0.0 0.0 0.0 0.7172896507324936 0.0 64-65 0.0 0.0 0.0 0.8291233174270087 0.0 66-67 0.0 0.0 0.0 0.9629797083785869 0.0 68-69 0.0 0.0 0.0 1.11465085535401 0.0 70-71 0.0 0.0 0.0 1.2856417980863266 0.0 72-73 0.0 0.0 0.0 1.471253126796833 0.0 74-75 0.0 0.0 0.0 1.6685054771160244 0.0 76-77 0.0 0.0 0.0 1.8796410510951775 0.0 78-79 0.0 0.0 0.0 2.132610586633773 0.0 80-81 0.0 0.0 0.0 2.410889361764865 0.0 82-83 0.0 0.0 0.0 2.704126388936668 0.0 84-85 0.0 0.0 0.0 3.0153317476152797 0.0 86-87 0.0 0.0 0.0 3.3449661642495934 0.0 88-89 0.0 6.143019318567153E-5 0.0 3.705254247283557 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 13300 0.0 26.651371 1 GAGTACT 5795 0.0 24.654123 12-13 GGTATCA 9735 0.0 24.371634 1 GTACTTT 6150 0.0 23.27382 14-15 GTACATG 9905 0.0 23.13893 1 TACATGG 9880 0.0 22.890274 2 TCAACGC 15510 0.0 22.834862 4 ATCAACG 15495 0.0 22.826366 3 CAACGCA 15560 0.0 22.67007 5 TATCAAC 15620 0.0 22.613344 2 AACGCAG 15850 0.0 22.274231 6 ATACCGT 175 4.1341045E-6 21.663452 6 AGTACTT 5955 0.0 21.56469 12-13 ACATGGG 10150 0.0 21.487274 3 ACGCAGA 17265 0.0 20.366344 7 CGCAGAG 17410 0.0 20.08846 8 CATGGGG 4780 0.0 20.036093 4 ACTTTTT 7210 0.0 19.759018 16-17 ACCGTAA 145 6.2440394E-4 19.609762 8 AGAGTAC 12915 0.0 19.424974 10-11 >>END_MODULE