FastQCFastQC Report
Fri 27 May 2016
SRR939305_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939305_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1627864
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62090.38142006948983453No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56980.35002924077195635No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT33300.2045625433082862No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30680.18846783269364026No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT19370.11899028420064575No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA141000.023.8003731
GGTATCA106650.023.2982581
GAGTACT59900.023.29154412-13
AGTACTT62050.022.06387512-13
GTACTTT62900.021.87888114-15
GTATAGG3451.8189894E-1220.6959781
TCAACGC164850.020.1505494
TATCAAC166300.020.117532
CAACGCA165750.020.0125055
ATCAACG165950.019.9883843
TACATGG98400.019.7727262
GTACATG99300.019.7497561
AACGCAG169850.019.6411786
GCCGCGC3153.255991E-1019.5844148
TACTTTT69850.019.12448714-15
AGAGTAC135850.018.93291310-11
ACTTTTT72400.018.87694516-17
TAGGACA8300.018.8674684
ACATGGG100200.018.801853
ACGCAGA184450.018.0093157
Produced by FastQC (version 0.11.5)