##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939305_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1627864 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.30307814412015 33.0 31.0 34.0 31.0 34.0 2 32.51743573173189 34.0 31.0 34.0 31.0 34.0 3 32.592872008963894 34.0 31.0 34.0 31.0 34.0 4 36.12656831283203 37.0 35.0 37.0 35.0 37.0 5 36.07224927880953 37.0 35.0 37.0 35.0 37.0 6 36.10333172795762 37.0 35.0 37.0 35.0 37.0 7 36.04991510347302 37.0 35.0 37.0 35.0 37.0 8 36.02197972312183 37.0 35.0 37.0 35.0 37.0 9 37.71807165709175 39.0 37.0 39.0 35.0 39.0 10-11 37.64377306703754 39.0 37.0 39.0 35.0 39.0 12-13 37.567043991389944 39.0 37.0 39.0 35.0 39.0 14-15 38.83697440326711 40.0 38.0 41.0 35.0 41.0 16-17 38.83485291154544 40.0 38.0 41.0 34.5 41.0 18-19 38.962172208489164 40.0 38.0 41.0 35.0 41.0 20-21 38.94825274101522 40.0 38.0 41.0 35.0 41.0 22-23 38.86901454912696 40.0 38.0 41.0 35.0 41.0 24-25 38.80050145466697 40.0 38.0 41.0 34.5 41.0 26-27 38.64274779711327 40.0 38.0 41.0 34.0 41.0 28-29 38.477862708432646 40.0 38.0 41.0 34.0 41.0 30-31 38.280519748578506 40.0 38.0 41.0 34.0 41.0 32-33 38.21915958581307 40.0 38.0 41.0 34.0 41.0 34-35 38.13121089968204 40.0 38.0 41.0 33.0 41.0 36-37 37.97786455133844 40.0 37.0 41.0 33.0 41.0 38-39 37.847207444847975 40.0 37.0 41.0 33.0 41.0 40-41 37.65959594904734 40.0 37.0 41.0 33.0 41.0 42-43 37.46096449089113 40.0 37.0 41.0 32.5 41.0 44-45 37.18896726016424 40.0 36.5 41.0 31.5 41.0 46-47 36.84751920307839 40.0 36.0 41.0 31.0 41.0 48-49 36.4827887956242 39.0 36.0 41.0 30.0 41.0 50-51 36.21053847250138 39.0 35.0 41.0 30.0 41.0 52-53 35.93714401203049 39.0 35.0 40.0 29.0 41.0 54-55 35.675566263520786 38.5 35.0 40.0 28.0 41.0 56-57 35.34264226004138 38.0 34.5 40.0 28.0 41.0 58-59 35.01300262184064 38.0 34.0 40.0 27.0 41.0 60-61 34.646235496331386 37.5 34.0 40.0 26.5 41.0 62-63 34.28088894403955 37.0 34.0 40.0 26.0 41.0 64-65 33.951556456804745 36.5 33.0 39.0 26.0 41.0 66-67 33.515451229341025 36.0 33.0 39.0 25.5 40.0 68-69 33.042109168824915 35.0 32.5 38.5 24.0 40.0 70-71 32.55330359292914 35.0 32.0 38.0 23.0 40.0 72-73 32.0593222161065 35.0 31.5 37.0 22.0 39.0 74-75 31.54512907712192 35.0 31.0 36.5 20.0 39.0 76-77 29.628669225438983 32.5 28.5 35.0 18.0 37.0 78-79 30.603876306620208 34.0 30.5 35.0 18.0 37.0 80-81 30.62718845063224 34.0 31.0 35.0 18.0 37.0 82-83 30.286156583105225 34.0 30.0 35.0 16.0 37.0 84-85 29.84520174904046 34.0 30.0 35.0 9.5 36.0 86-87 29.254656408643473 34.0 29.0 35.0 4.5 36.0 88-89 28.664658718418735 34.0 29.0 35.0 2.0 35.0 90-91 28.171449826275413 33.0 27.5 35.0 2.0 35.0 92-93 27.804175901672373 33.0 27.0 35.0 2.0 35.0 94-95 27.2735179351592 33.0 26.5 35.0 2.0 35.0 96-97 26.52250126546198 32.5 25.0 34.0 2.0 35.0 98-99 25.496880267639064 32.0 22.0 34.0 2.0 35.0 100-101 21.157025402613485 27.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2788117652174691 1101 2 0.29244400553538696 1101 3 0.2995613422489072 1101 4 0.1879441190139559 1101 5 0.1918545699405101 1101 6 0.2258433392111101 1101 7 0.2424847700232391 1101 8 0.25230756945810384 1101 9 0.39853901165430017 1101 10-11 0.347640806967064 1101 12-13 0.3136985165536217 1101 14-15 0.3937183708680507 1101 16-17 0.3650496356288073 1101 18-19 0.35719146093825316 1101 20-21 0.35000044101769134 1101 22-23 0.2418993302836654 1101 24-25 0.22908550265206173 1101 26-27 0.24129826798577625 1101 28-29 0.25716608670895624 1101 30-31 0.38444266448193076 1101 32-33 0.45403642851814396 1101 34-35 0.38760912906123224 1101 36-37 0.44503063712033253 1101 38-39 0.38566883360034865 1101 40-41 0.4834036296617157 1101 42-43 0.5406843867685538 1101 44-45 0.5245494081750834 1101 46-47 0.6053291159976553 1101 48-49 0.7449200949940504 1101 50-51 0.8233413325408989 1101 52-53 0.7719925979110585 1101 54-55 0.6730553180234438 1101 56-57 0.7002678518861458 1101 58-59 0.7026057350250454 1101 60-61 0.5261752175612457 1101 62-63 0.5909256334846233 1101 64-65 0.5803852734331443 1101 66-67 0.5942174637013267 1101 68-69 0.6312973276500884 1101 70-71 0.6484845482674118 1101 72-73 0.6893066262272924 1101 74-75 0.555050945350704 1101 76-77 0.6713430402502318 1101 78-79 0.7965966506322815 1101 80-81 0.8919317830149964 1101 82-83 0.8032383563945338 1101 84-85 0.7220775372649832 1101 86-87 0.7638817163520706 1101 88-89 0.7936386888491427 1101 90-91 1.142018535961153 1101 92-93 0.9568300441547244 1101 94-95 0.5779953030526706 1101 96-97 0.7031581904384829 1101 98-99 0.7408099138979765 1101 100-101 0.8004873457419031 1102 1 0.22745261786243276 1102 2 0.24920956201860633 1102 3 0.23481004289801888 1102 4 0.14436274316872044 1102 5 0.0950778439286708 1102 6 0.15892911893446637 1102 7 0.19146597362456674 1102 8 0.24581645619080916 1102 9 0.247489470846034 1102 10-11 0.33834179830973454 1102 12-13 0.25069950752021697 1102 14-15 0.412686355313447 1102 16-17 0.31942873958439577 1102 18-19 0.3703405722649791 1102 20-21 0.4354567369462572 1102 22-23 0.42175459650956526 1102 24-25 0.3544458327736919 1102 26-27 0.42432364011536094 1102 28-29 0.34383711588512256 1102 30-31 0.5206371082995744 1102 32-33 0.4638993197337058 1102 34-35 0.5397034626879105 1102 36-37 0.6864165612578006 1102 38-39 0.6136908116223267 1102 40-41 0.6507411261829859 1102 42-43 0.6750344010595342 1102 44-45 0.6923480708402749 1102 46-47 0.6422313393731756 1102 48-49 0.7843349922118179 1102 50-51 0.661163866782843 1102 52-53 0.7447487731184665 1102 54-55 0.711709596667454 1102 56-57 0.591807780130388 1102 58-59 0.5979964710857075 1102 60-61 0.7290307338792843 1102 62-63 0.8448022966913769 1102 64-65 0.647224042979822 1102 66-67 0.5187458143718757 1102 68-69 0.5542463840693657 1102 70-71 0.42831978831825523 1102 72-73 0.5668167145305034 1102 74-75 0.45596885871728077 1102 76-77 0.3268568667727827 1102 78-79 0.42374429337283814 1102 80-81 0.6474352203716123 1102 82-83 0.6915618740815042 1102 84-85 0.8028326180842313 1102 86-87 0.5234496210688455 1102 88-89 0.8230412036854382 1102 90-91 0.7988895354271222 1102 92-93 0.8089456732210714 1102 94-95 0.627903257863089 1102 96-97 0.2658436441018637 1102 98-99 0.007441746302397689 1102 100-101 -0.006636378412107291 1103 1 0.18103972467434915 1103 2 0.21837575450930302 1103 3 0.24134679909460033 1103 4 0.13392125098506114 1103 5 0.18544891067340075 1103 6 0.1686888972675078 1103 7 0.16973222136707733 1103 8 0.17381043071999613 1103 9 0.28261285280645154 1103 10-11 0.15476766605175385 1103 12-13 0.21730240095569542 1103 14-15 0.36641777654937613 1103 16-17 0.3538339854824386 1103 18-19 0.240994990073105 1103 20-21 0.3446273001023812 1103 22-23 0.2693029006089134 1103 24-25 0.25375147423068967 1103 26-27 0.2724498891223348 1103 28-29 0.24047294049774592 1103 30-31 0.32838418146561565 1103 32-33 0.3417296004733643 1103 34-35 0.30246498104058617 1103 36-37 0.300956170756713 1103 38-39 0.26128117306810594 1103 40-41 0.37814835949680514 1103 42-43 0.34782549699701093 1103 44-45 0.2901245291718837 1103 46-47 0.3602471397409701 1103 48-49 0.41007889870706293 1103 50-51 0.4917792784637882 1103 52-53 0.11676868026244591 1103 54-55 0.25043259268987583 1103 56-57 0.40140757561623275 1103 58-59 0.30626073965896694 1103 60-61 0.44346203883903 1103 62-63 0.421678590367101 1103 64-65 0.4531101472875392 1103 66-67 0.43002268090715035 1103 68-69 0.5231026441541999 1103 70-71 0.43575266836685245 1103 72-73 0.4697089130301819 1103 74-75 0.42468424043580555 1103 76-77 0.40652598815908547 1103 78-79 0.23233732394825424 1103 80-81 0.4357122161026581 1103 82-83 0.6904465297472875 1103 84-85 0.5721927416723531 1103 86-87 0.2902794317558097 1103 88-89 0.4152409403459707 1103 90-91 0.5892961997297803 1103 92-93 0.33377187742185654 1103 94-95 0.3746204483223927 1103 96-97 0.024934765603614295 1103 98-99 -0.1671732090700857 1103 100-101 -0.2306900193963024 1104 1 0.15278222815413756 1104 2 0.018650411786524046 1104 3 0.1115047004149261 1104 4 0.1086019485932539 1104 5 0.1569026514150309 1104 6 0.05110923468077999 1104 7 0.08522321117122544 1104 8 0.10669771211862411 1104 9 0.14589234586151179 1104 10-11 0.18766066947699045 1104 12-13 0.11579231821253444 1104 14-15 0.01157760739030067 1104 16-17 0.04748000760937998 1104 18-19 0.08749963023747398 1104 20-21 0.12139041618500102 1104 22-23 0.14564125601862798 1104 24-25 0.17759795358747965 1104 26-27 0.2401324151176354 1104 28-29 0.21802729309700197 1104 30-31 0.12545583454579656 1104 32-33 0.12331585270562329 1104 34-35 0.13176029350316298 1104 36-37 0.25699038049160805 1104 38-39 0.2893038003565849 1104 40-41 0.2568886353783313 1104 42-43 0.2618407362176427 1104 44-45 0.3991224825493518 1104 46-47 0.30320186121134896 1104 48-49 0.13325856235835687 1104 50-51 0.2311096948942577 1104 52-53 0.21081684109663712 1104 54-55 0.1932828539070428 1104 56-57 -0.012374790428459903 1104 58-59 -0.001596933578710491 1104 60-61 0.14813826528224183 1104 62-63 0.32776216921529766 1104 64-65 0.19788380786756932 1104 66-67 0.24789589676593948 1104 68-69 0.20660737214502234 1104 70-71 0.15225951749963684 1104 72-73 0.24367888170541363 1104 74-75 0.2157238129072674 1104 76-77 0.32474292482556066 1104 78-79 0.5896945194632544 1104 80-81 0.5667359798122327 1104 82-83 0.4957572357921407 1104 84-85 0.2999580108338229 1104 86-87 0.40876197691324734 1104 88-89 0.5544372218270972 1104 90-91 0.5474515514215064 1104 92-93 0.5635059229478969 1104 94-95 0.5434721307312849 1104 96-97 0.5679590828859951 1104 98-99 0.5076227274926026 1104 100-101 0.5067814037382483 1105 1 0.0924833877126261 1105 2 0.09217736041580338 1105 3 0.05861111600456326 1105 4 0.09910281326088466 1105 5 0.13606109870585925 1105 6 0.10492381755629054 1105 7 0.14746214558885384 1105 8 0.06872644470050915 1105 9 0.12288451908803921 1105 10-11 0.19827913922303964 1105 12-13 0.17041716555168307 1105 14-15 0.29097919827593444 1105 16-17 0.1591874830754776 1105 18-19 0.20168887528861035 1105 20-21 0.16835855349344087 1105 22-23 0.23555640849956916 1105 24-25 0.2081781012299615 1105 26-27 0.3471685006942451 1105 28-29 0.35024947390999017 1105 30-31 0.3307500335834135 1105 32-33 0.3148355558613929 1105 34-35 0.4258888640321459 1105 36-37 0.23171454145130355 1105 38-39 0.3079377411633786 1105 40-41 0.32822425021763024 1105 42-43 0.32548965502970617 1105 44-45 0.11213828988160657 1105 46-47 0.1944936279562839 1105 48-49 0.28936094825842673 1105 50-51 0.2761330261414159 1105 52-53 0.22660232641784006 1105 54-55 0.27591569294264673 1105 56-57 0.30345143301090616 1105 58-59 0.35433084814722093 1105 60-61 0.2734077644261461 1105 62-63 0.18144357142903544 1105 64-65 0.20330463348486916 1105 66-67 0.29304988128374276 1105 68-69 0.25605492299592214 1105 70-71 0.2443959897670851 1105 72-73 0.1475928253223131 1105 74-75 0.14211461697319905 1105 76-77 0.3690442361131936 1105 78-79 0.34138091049007 1105 80-81 0.4166166569645746 1105 82-83 0.5217257513525162 1105 84-85 0.5424354881376381 1105 86-87 0.4743293569467717 1105 88-89 0.5132371051387352 1105 90-91 0.6087484971764106 1105 92-93 0.600414400974369 1105 94-95 0.779393169891712 1105 96-97 0.7365898323260076 1105 98-99 0.7877354148675977 1105 100-101 0.5582240942874748 1106 1 0.10185758731989836 1106 2 0.07380501873602441 1106 3 0.10124425053558639 1106 4 0.08176798382838513 1106 5 0.045141770161670536 1106 6 0.07979748255204555 1106 7 0.06372327994099436 1106 8 0.007176599798832228 1106 9 0.18764935100048774 1106 10-11 0.08896104333447141 1106 12-13 0.15821826567242425 1106 14-15 0.19182380317159442 1106 16-17 0.09154205044669084 1106 18-19 0.19999072142618957 1106 20-21 0.06152507990081091 1106 22-23 0.18208610891383614 1106 24-25 0.1839022660773182 1106 26-27 0.08829313632498526 1106 28-29 0.10382445978242316 1106 30-31 0.12039625491664907 1106 32-33 0.13219965679930112 1106 34-35 0.12485643364344412 1106 36-37 0.016362449961583536 1106 38-39 0.11966565438962817 1106 40-41 0.22910564776939424 1106 42-43 0.34901642422794765 1106 44-45 0.34714253831537434 1106 46-47 0.27400784293109126 1106 48-49 0.0864813639138049 1106 50-51 0.13225564935616063 1106 52-53 0.20631554697457233 1106 54-55 0.0810430849734729 1106 56-57 0.03276318673997736 1106 58-59 0.18074736540724246 1106 60-61 0.14238098506417884 1106 62-63 0.1579633520553685 1106 64-65 0.138661156355262 1106 66-67 0.20083549189954653 1106 68-69 0.18650846871027937 1106 70-71 0.14307125087297834 1106 72-73 0.1741695227647213 1106 74-75 0.20137843861215998 1106 76-77 0.3429862947944322 1106 78-79 0.46832024936059824 1106 80-81 0.3588412100659326 1106 82-83 0.36004946980942165 1106 84-85 0.34135366208355933 1106 86-87 0.3913844808831364 1106 88-89 0.5045503793598911 1106 90-91 0.682554768596443 1106 92-93 0.6201740900459392 1106 94-95 0.861099698657064 1106 96-97 0.7717345433308758 1106 98-99 0.7761030405826155 1106 100-101 0.8048470976252382 1107 1 0.13434626772914982 1107 2 0.15088803682797192 1107 3 0.10470977185754293 1107 4 0.04779058809084091 1107 5 0.139481780792579 1107 6 0.04500724902122499 1107 7 0.09765489292932727 1107 8 0.014826974790587144 1107 9 0.11380643238902621 1107 10-11 0.19290107569278803 1107 12-13 0.09230973029361422 1107 14-15 0.16978464728132536 1107 16-17 0.19199580405014416 1107 18-19 0.1995468615524416 1107 20-21 0.2686336276952872 1107 22-23 0.2424310702894914 1107 24-25 0.2158206020683835 1107 26-27 0.1849764089379775 1107 28-29 0.2230330390040436 1107 30-31 0.3531920289360997 1107 32-33 0.24460988575184928 1107 34-35 0.36196082995134304 1107 36-37 0.2593055047573287 1107 38-39 0.35767600594682136 1107 40-41 0.5030475036778839 1107 42-43 0.35988070941568395 1107 44-45 0.45519525831506513 1107 46-47 0.47835002630264256 1107 48-49 0.5295039565365798 1107 50-51 0.6221925622797002 1107 52-53 0.5489780854340225 1107 54-55 0.6284154267610944 1107 56-57 0.7325408688137003 1107 58-59 0.6350385281726787 1107 60-61 0.7194343219670216 1107 62-63 0.7316755182389798 1107 64-65 0.7703899595243584 1107 66-67 0.6875458849716054 1107 68-69 0.7519900217542599 1107 70-71 0.7827821599709424 1107 72-73 0.7904132809852413 1107 74-75 0.6059179145936717 1107 76-77 0.6132234339157883 1107 78-79 0.7593784516505089 1107 80-81 0.6985864562258044 1107 82-83 0.9458921245880916 1107 84-85 0.8997115357464516 1107 86-87 0.9681058928541617 1107 88-89 1.0722219385728913 1107 90-91 1.1137776335877696 1107 92-93 0.9954178443865693 1107 94-95 1.3279719997057988 1107 96-97 1.262228997022401 1107 98-99 1.1616122184718733 1107 100-101 1.1124206507157695 1108 1 0.020561786590477027 1108 2 0.008112799637757462 1108 3 -0.041474492054433654 1108 4 0.035991530907864444 1108 5 0.09023622269828024 1108 6 0.08595581052195911 1108 7 0.011393300152597874 1108 8 0.0076689047117142195 1108 9 0.14456815456405536 1108 10-11 0.05219399614772158 1108 12-13 0.10257538997941396 1108 14-15 0.03233338392201546 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-1.3528474161106097 2316 92-93 -1.2087567275641256 2316 94-95 -1.1278774612237719 2316 96-97 -0.7134690316442978 2316 98-99 -0.6936089753061623 2316 100-101 -0.08813535851511034 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 3.0 4 15.0 5 34.0 6 77.0 7 174.0 8 432.0 9 864.0 10 1357.0 11 2188.0 12 3085.0 13 4288.0 14 5516.0 15 7816.0 16 10570.0 17 15093.0 18 14918.0 19 9917.0 20 8734.0 21 9568.0 22 10254.0 23 11127.0 24 12734.0 25 15236.0 26 18467.0 27 22252.0 28 26948.0 29 33029.0 30 40101.0 31 48971.0 32 60904.0 33 79570.0 34 109219.0 35 159033.0 36 250880.0 37 375161.0 38 246286.0 39 13041.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.5970945115471 19.961863923826424 16.89569875580281 21.545342808823666 2 15.554309205191588 21.032776693876144 38.706673284746145 24.706240816186117 3 18.987704132531956 25.371959819739242 30.370104627905032 25.27023141982377 4 13.424462977251169 17.843443924062452 36.77303509384076 31.959058004845613 5 14.011674193913004 37.6886521232732 33.33331285660227 14.966360826211526 6 30.92660074797403 36.40371677240851 17.350896635099737 15.318785844517723 7 28.040855992883927 33.41028488866392 20.35722885941332 18.19163025903884 8 27.21302270951382 33.65139839691768 19.56729800523877 19.56828088832974 9 26.920430699370463 16.600403964950388 20.202916214130912 36.27624912154824 10-11 25.519638004157596 26.891005636834524 26.123036076723853 21.466320282284023 12-13 26.635640323761688 24.13466358369004 26.78663573861207 22.4430603539362 14-15 23.53259894682783 25.116288784428974 25.90423283803608 25.44687943070712 16-17 22.224938822736597 28.463130697118256 26.46595835062351 22.84597212952164 18-19 22.291570169952983 26.83691860857984 28.999479071321772 21.872032150145408 20-21 23.769461085201222 25.440945926686748 28.82836035442764 21.961232633684386 22-23 23.207774113808032 25.563284156416017 28.798535995635998 22.430405734139953 24-25 22.959012546502656 25.790698731589373 28.9438798327133 22.306408889194675 26-27 22.515008941170112 25.866058753101456 29.15417329345283 22.464759012275604 28-29 22.40503506435427 26.25243877866947 29.02794705208789 22.31457910488837 30-31 22.601550252355235 25.83907500872309 28.82842178462083 22.73095295430085 32-33 22.47577193180757 25.927104475558156 28.74653533710433 22.850588255529946 34-35 23.123279929231373 25.89130381364262 28.57031403577747 22.415102221348533 36-37 23.156139617421797 25.55704468736782 28.765552049969294 22.52126364524109 38-39 22.750635132673693 25.87762880115886 28.70464879336311 22.66708727280434 40-41 22.897713555197747 25.795562261220745 28.771399136631953 22.535325046949552 42-43 22.69523674376803 25.787185238015383 28.34299941579635 23.174578602420237 44-45 22.851936126322155 26.009129901132667 28.178387529963057 22.960546442582125 46-47 23.209671850984545 26.27799844949621 27.683372874046754 22.828956825472492 48-49 23.10287517012455 26.220512129024055 27.59219744552866 23.08441525532274 50-51 23.0888830666699 26.51718034098068 27.5389490580752 22.85498753427422 52-53 23.262799142188257 26.5729364203253 27.12851142878731 23.035753008699135 54-55 22.987761876913552 26.51787864342476 27.248990087624026 23.245369392037667 56-57 23.15085251940979 26.623312998759406 27.132266057438287 23.09356842439252 58-59 23.277872091955352 26.17518929300816 27.281874958341866 23.26506365669462 60-61 23.047904202652205 26.415407288004506 27.618802189141157 22.91788632020213 62-63 22.931138120908894 26.46909672938863 27.685643385689858 22.914121764012616 64-65 22.84576598536487 26.53839632794877 27.483223414240992 23.132614272445366 66-67 22.784242478226748 26.517677376551696 27.262613588720143 23.435466556501414 68-69 22.95109309129443 26.374438480499435 27.400032251376444 23.274436176829692 70-71 23.07354914169734 26.406966429628024 27.136941415253364 23.38254301342127 72-73 22.9373891185013 26.266352717426027 27.335852380788566 23.46040578328411 74-75 22.708107897253054 26.95530061334995 26.899706271441065 23.436885217955925 76-77 22.909675359010738 26.421607378940603 27.120920353524454 23.54779690852421 78-79 22.920287849687643 26.51008515148844 27.22098628048359 23.348640718340327 80-81 22.956484346804476 26.66260203180551 27.017146718472844 23.363766902917167 82-83 23.009753886074023 26.52202518146479 27.129109065622192 23.339111866838998 84-85 22.701128595509207 26.65198075514908 27.060767975703133 23.586122673638585 86-87 22.830318577959154 26.644509160188683 27.02717661525178 23.49799564660038 88-89 22.793473334340536 26.530303917911024 26.890152344364243 23.786070403384198 90-91 22.675870438734698 26.41439460094656 27.118569411010952 23.79116554930779 92-93 22.723980627374278 26.401284136758353 27.178652516426432 23.696082719440938 94-95 22.483512136148967 26.350849948152916 27.30123646692844 23.864401448769677 96-97 22.362187504607263 26.234132581100138 27.374092676046647 24.02958723824595 98-99 22.36226954057852 26.343994041265773 26.995001113423278 24.298735304732432 100-101 22.534295004303193 25.67562586731914 27.642808569036298 24.14727055934137 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 163.0 1 124.5 2 120.5 3 172.5 4 173.0 5 171.5 6 329.0 7 726.0 8 1315.0 9 1712.5 10 1613.0 11 1405.5 12 1433.5 13 1592.0 14 1740.0 15 1857.5 16 1995.0 17 2169.5 18 2301.0 19 2422.0 20 2664.0 21 3026.0 22 3455.5 23 4091.0 24 4944.0 25 5990.5 26 7628.5 27 9515.5 28 11195.5 29 13343.5 30 15866.5 31 18947.0 32 22431.0 33 25307.0 34 27713.5 35 30104.5 36 33329.0 37 36739.5 38 39592.5 39 41697.0 40 43171.5 41 45602.5 42 48124.0 43 50340.5 44 53094.0 45 56057.0 46 57933.5 47 62465.5 48 71680.5 49 71529.0 50 63873.0 51 60252.0 52 59011.5 53 59483.5 54 59513.5 55 56553.5 56 51935.0 57 47130.0 58 42990.5 59 38378.0 60 32202.5 61 27072.5 62 22260.5 63 16447.0 64 11863.5 65 9127.5 66 6935.0 67 5376.5 68 4209.0 69 3244.0 70 2494.0 71 1721.5 72 1285.5 73 1054.0 74 810.5 75 604.5 76 404.0 77 238.0 78 132.5 79 87.5 80 49.0 81 31.5 82 25.5 83 14.0 84 6.5 85 4.5 86 3.0 87 1.0 88 1.5 89 1.5 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2905033835750407 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0012286038637134306 16-17 7.678774148208941E-4 18-19 1.2286038637134307E-4 20-21 0.0 22-23 0.0 24-25 0.0 26-27 6.143019318567153E-5 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0014743246364561167 36-37 0.008876662915329537 38-39 0.0033479455286190988 40-41 0.003255800238840591 42-43 4.300113522997007E-4 44-45 0.0015971850228274599 46-47 3.685811591140292E-4 48-49 7.678774148208941E-4 50-51 3.071509659283577E-5 52-53 6.143019318567153E-5 54-55 0.0 56-57 7.678774148208941E-4 58-59 0.0018736208921629818 60-61 6.757321250423868E-4 62-63 0.0011364585739349234 64-65 0.0 66-67 3.9929625570686496E-4 68-69 0.0016279001194202955 70-71 0.0 72-73 0.0 74-75 3.071509659283577E-5 76-77 0.2623376399994103 78-79 3.071509659283577E-5 80-81 9.21452897785073E-5 82-83 0.0 84-85 0.0 86-87 0.10925359858071683 88-89 0.38142006948983453 90-91 0.2835924868416526 92-93 0.0 94-95 0.0 96-97 0.0 98-99 9.21452897785073E-5 100-101 1.842905795570146E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1627864.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.77542235489648 #Duplication Level Percentage of deduplicated Percentage of total 1 74.10236068503188 40.589881040174966 2 14.457795095285912 15.838636653296728 3 5.001139418371578 8.218185716910734 4 2.217574939691158 4.85874415700869 5 1.1559911743324116 3.16599524062953 6 0.6878899378717611 2.2607677128365524 7 0.46268782783146967 1.774074483155686 8 0.32167839419991223 1.4096055923796058 9 0.23791848351964237 1.17288768787424 >10 1.24550227456868 12.22990673810114 >50 0.07576102795536874 2.814606912524088 >100 0.03023013373604039 3.1442119919644878 >500 0.002239101680078935 0.8456561688298762 >1k 0.0010075957560355207 0.9466565829025959 >5k 2.239101680078935E-4 0.7301833214109742 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6209 0.38142006948983453 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5698 0.35002924077195635 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3330 0.2045625433082862 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3068 0.18846783269364026 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1937 0.11899028420064575 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 6.143019318567153E-5 0.0 20-21 0.0 0.0 0.0 1.5357548296417883E-4 0.0 22-23 0.0 0.0 0.0 1.842905795570146E-4 0.0 24-25 0.0 0.0 0.0 1.842905795570146E-4 0.0 26-27 0.0 0.0 0.0 3.992962557068649E-4 0.0 28-29 0.0 0.0 0.0 9.828830909707445E-4 0.0 30-31 0.0 0.0 0.0 0.002917934176319398 0.0 32-33 0.0 0.0 0.0 0.006296594801531332 0.0 34-35 0.0 0.0 0.0 0.012071032960984456 0.0 36-37 0.0 0.0 0.0 0.0223298752229916 0.0 38-39 0.0 0.0 0.0 0.03913103305927276 0.0 40-41 0.0 0.0 0.0 0.05986372325943691 0.0 42-43 0.0 0.0 0.0 0.0825314645449497 0.0 44-45 0.0 0.0 0.0 0.1131851309445998 0.0 46-47 0.0 0.0 0.0 0.15001253175940987 0.0 48-49 0.0 0.0 0.0 0.1947644274951716 0.0 50-51 0.0 0.0 0.0 0.2462736444813572 0.0 52-53 0.0 0.0 0.0 0.298857889848292 0.0 54-55 0.0 0.0 0.0 0.3617624076704197 0.0 56-57 0.0 0.0 0.0 0.43366644879424815 0.0 58-59 0.0 0.0 0.0 0.5172115115267615 0.0 60-61 0.0 0.0 0.0 0.6174041566125917 0.0 62-63 0.0 0.0 0.0 0.7202997301985916 0.0 64-65 0.0 0.0 0.0 0.8327784139215562 0.0 66-67 0.0 0.0 0.0 0.9679248389300334 0.0 68-69 0.0 0.0 0.0 1.1205174388032417 0.0 70-71 0.0 0.0 0.0 1.2921841136606007 0.0 72-73 0.0 0.0 0.0 1.4786861801722995 0.0 74-75 0.0 0.0 0.0 1.6766449777131258 0.0 76-77 0.0 0.0 0.0 1.8882412781411715 0.0 78-79 0.0 0.0 0.0 2.1420708363843666 0.0 80-81 0.0 0.0 0.0 2.4212710644132436 0.0 82-83 0.0 0.0 0.0 2.7157059803521673 0.0 84-85 0.0 0.0 0.0 3.027372065479671 0.0 86-87 0.0 0.0 0.0 3.3566993311480564 0.0 88-89 0.0 0.0 0.0 3.7176631463070624 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 14100 0.0 23.800373 1 GGTATCA 10665 0.0 23.298258 1 GAGTACT 5990 0.0 23.291544 12-13 AGTACTT 6205 0.0 22.063875 12-13 GTACTTT 6290 0.0 21.878881 14-15 GTATAGG 345 1.8189894E-12 20.695978 1 TCAACGC 16485 0.0 20.150549 4 TATCAAC 16630 0.0 20.11753 2 CAACGCA 16575 0.0 20.012505 5 ATCAACG 16595 0.0 19.988384 3 TACATGG 9840 0.0 19.772726 2 GTACATG 9930 0.0 19.749756 1 AACGCAG 16985 0.0 19.641178 6 GCCGCGC 315 3.255991E-10 19.584414 8 TACTTTT 6985 0.0 19.124487 14-15 AGAGTAC 13585 0.0 18.932913 10-11 ACTTTTT 7240 0.0 18.876945 16-17 TAGGACA 830 0.0 18.867468 4 ACATGGG 10020 0.0 18.80185 3 ACGCAGA 18445 0.0 18.009315 7 >>END_MODULE