FastQCFastQC Report
Fri 27 May 2016
SRR939304_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939304_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1660767
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63980.3852436855982808No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52460.3158781454592968No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT32110.19334440050892146No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27090.1631174029830795No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT21420.12897655119592333No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATACG550.002581372634.541581
GTATCAA128800.027.2873121
GGTATCA93050.024.9595831
GAGTACT58850.023.1182612-13
TATCAAC150750.023.0608752
ATCAACG152100.022.8561923
TCAACGC153000.022.752744
CAACGCA154100.022.5595535
AACGCAG156800.022.1892956
GTACTTT60950.022.04951514-15
GAGTACG1954.2283318E-721.9206181
GTACATG98900.021.7063641
TACATGG99600.021.1899432
AGTACTT60350.020.93339312-13
ACGCAGA171700.020.1256897
CGCAGAG172350.019.994788
CGTAAGA2151.095339E-619.8533212
ACATGGG103450.019.621963
AGAGTAC131450.019.27497110-11
GCAGAGT180350.018.6084699
Produced by FastQC (version 0.11.5)