##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939304_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1660767 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.786682297998453 33.0 31.0 34.0 30.0 34.0 2 31.878507942414558 33.0 31.0 34.0 30.0 34.0 3 31.91463040872079 34.0 31.0 34.0 30.0 34.0 4 35.42165999203982 37.0 35.0 37.0 33.0 37.0 5 35.466125591368325 37.0 35.0 37.0 33.0 37.0 6 35.55703418962443 37.0 35.0 37.0 33.0 37.0 7 35.48017452177217 37.0 35.0 37.0 33.0 37.0 8 35.45729653828622 37.0 35.0 37.0 33.0 37.0 9 37.07232140330341 39.0 37.0 39.0 33.0 39.0 10-11 36.97591895792727 39.0 37.0 39.0 33.0 39.0 12-13 36.87100899764988 39.0 37.0 39.0 33.0 39.0 14-15 38.07261584557015 40.0 38.0 41.0 33.0 41.0 16-17 38.00685345987728 40.0 38.0 41.0 33.0 41.0 18-19 37.97280985231522 40.0 38.0 41.0 33.0 41.0 20-21 37.879948240782724 40.0 38.0 41.0 32.5 41.0 22-23 37.793803104228346 40.0 38.0 41.0 32.0 41.0 24-25 37.8067338765763 40.0 38.0 41.0 32.0 41.0 26-27 37.71730170457385 40.0 38.0 41.0 32.0 41.0 28-29 37.58738643048663 40.0 37.5 41.0 32.0 41.0 30-31 37.407534590945026 40.0 37.0 41.0 31.5 41.0 32-33 37.19881837729194 40.0 36.5 41.0 31.0 41.0 34-35 36.899960379752244 40.0 36.0 41.0 30.5 41.0 36-37 36.65282757906437 39.5 36.0 41.0 30.0 41.0 38-39 36.32590875179962 39.0 35.5 41.0 30.0 41.0 40-41 36.19108159061446 39.0 35.0 41.0 30.0 41.0 42-43 36.018878325496594 39.0 35.0 41.0 29.0 41.0 44-45 35.95462156943147 39.0 35.0 41.0 28.5 41.0 46-47 35.729967238029175 39.0 35.0 41.0 27.0 41.0 48-49 35.493395822532605 39.0 35.0 41.0 26.0 41.0 50-51 34.329590785462386 38.0 34.0 40.0 24.5 40.5 52-53 34.42831504961262 38.0 34.0 39.5 24.0 40.5 54-55 34.917161167099295 39.0 35.0 40.0 25.0 41.0 56-57 34.80288776210028 38.5 34.5 40.0 24.5 41.0 58-59 34.58143466241803 38.0 34.0 40.0 24.0 41.0 60-61 34.26872884637038 38.0 34.0 40.0 23.0 41.0 62-63 33.90001577584333 37.0 34.0 40.0 22.5 41.0 64-65 33.510538203131446 37.0 33.0 40.0 22.0 41.0 66-67 33.13421509459184 36.0 33.0 39.0 21.0 41.0 68-69 32.72215789451501 35.5 32.5 39.0 20.0 40.5 70-71 32.29499502338378 35.0 32.0 38.5 18.5 40.0 72-73 31.85935655031681 35.0 32.0 37.5 17.5 40.0 74-75 31.428019403083034 35.0 31.0 37.0 14.0 39.0 76-77 30.99412711114804 35.0 31.0 36.5 9.5 39.0 78-79 30.58302188085385 35.0 31.0 36.0 7.0 38.5 80-81 30.139972675275942 34.0 30.0 35.5 4.5 37.0 82-83 29.713947832537617 34.0 30.0 35.0 2.0 37.0 84-85 29.314677194332496 34.0 29.0 35.0 2.0 36.0 86-87 29.00375007451376 34.0 29.0 35.0 2.0 36.0 88-89 28.605835436277335 34.0 29.0 35.0 2.0 36.0 90-91 28.214836578520647 34.0 29.0 35.0 2.0 35.0 92-93 27.809872787693877 34.0 27.5 35.0 2.0 35.0 94-95 27.336874468242684 34.0 26.5 35.0 2.0 35.0 96-97 26.766258000068643 33.0 25.5 35.0 2.0 35.0 98-99 26.03630461106224 33.0 24.5 35.0 2.0 35.0 100-101 23.611019787845013 31.0 10.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.33243740853381 1101 2 0.15156959653589297 1101 3 0.33406482143032434 1101 4 0.46706744283793 1101 5 0.4007988476109148 1101 6 0.3386563675132024 1101 7 0.3051644302924359 1101 8 0.32974802756687893 1101 9 0.4385633773590527 1101 10-11 0.5577858661155162 1101 12-13 0.5187833181366273 1101 14-15 0.46866669871929645 1101 16-17 0.697876896361187 1101 18-19 0.7715561470395329 1101 20-21 0.721911710808314 1101 22-23 0.5900895565673849 1101 24-25 0.6407020641590222 1101 26-27 0.6408691211344504 1101 28-29 0.6253850747322574 1101 30-31 0.7249005967245026 1101 32-33 0.7208936462655942 1101 34-35 0.626164326176962 1101 36-37 0.49879107289797275 1101 38-39 0.7439019612208071 1101 40-41 0.7624529990794926 1101 42-43 0.4968995020674001 1101 44-45 0.7122082867789459 1101 46-47 0.7144561156857208 1101 48-49 0.7644595224109167 1101 50-51 0.832747438780082 1101 52-53 0.8379022185419416 1101 54-55 0.8042297857972116 1101 56-57 0.8574856374063273 1101 58-59 0.8296461058199114 1101 60-61 0.8837635092824812 1101 62-63 1.1269361180177597 1101 64-65 1.1173980421119083 1101 66-67 0.9500705589461731 1101 68-69 0.8190016933773521 1101 70-71 0.8129927329174649 1101 72-73 0.7870883792652315 1101 74-75 0.7040471968090714 1101 76-77 0.6834435326193535 1101 78-79 0.6587794431293403 1101 80-81 0.6282380035288995 1101 82-83 0.6109957564074477 1101 84-85 0.5129201989263237 1101 86-87 0.5511273530137792 1101 88-89 0.5048361072826495 1101 90-91 0.5688006440830762 1101 92-93 0.3843685337043148 1101 94-95 0.6850441935420797 1101 96-97 0.7459211880743588 1101 98-99 0.48624050574361277 1101 100-101 0.4710731242407533 1102 1 0.3254568236999624 1102 2 0.21170903439271882 1102 3 0.33097916413270667 1102 4 0.35218171966416634 1102 5 0.24821000629924583 1102 6 0.24886488383584293 1102 7 0.2449147413742736 1102 8 0.385130200747966 1102 9 0.448755882116771 1102 10-11 0.32791217448910714 1102 12-13 0.3209606622297301 1102 14-15 0.39863005510928673 1102 16-17 0.5076256520338518 1102 18-19 0.486570877679263 1102 20-21 0.4673861563506989 1102 22-23 0.45321720991242387 1102 24-25 0.3993492241648795 1102 26-27 0.27911251522024116 1102 28-29 0.3937233487770513 1102 30-31 0.45150198195361213 1102 32-33 0.5292528268394463 1102 34-35 0.4744988946881534 1102 36-37 0.41449201346303965 1102 38-39 0.42853085889317555 1102 40-41 0.526907324946869 1102 42-43 0.38615322589992473 1102 44-45 0.2906695296423649 1102 46-47 0.3242725774043578 1102 48-49 0.24655905029848668 1102 50-51 0.23852944361100015 1102 52-53 0.3468865363577862 1102 54-55 0.3734626488480046 1102 56-57 0.44484450689834887 1102 58-59 0.3067376736723517 1102 60-61 0.2716475581040285 1102 62-63 0.08540898070257441 1102 64-65 0.14189710886640938 1102 66-67 0.07854323864167867 1102 68-69 0.09812078282253367 1102 70-71 0.12507666096607295 1102 72-73 0.15659732744265753 1102 74-75 0.17830723377291235 1102 76-77 0.22608438864336122 1102 78-79 0.07149994525201464 1102 80-81 -0.29909739758367593 1102 82-83 -0.1818205406208051 1102 84-85 -0.09770004335153004 1102 86-87 -0.10056781423845251 1102 88-89 -0.20346634660404206 1102 90-91 -0.16491170586568416 1102 92-93 0.003305182804005824 1102 94-95 -0.028047319413563088 1102 96-97 -0.24409377130201548 1102 98-99 -0.31812516180140094 1102 100-101 -0.5288003843963409 1103 1 0.09213627986058626 1103 2 0.2859163866966341 1103 3 0.3179054424802459 1103 4 0.3446159064329066 1103 5 0.18849194693191862 1103 6 0.14982229030702854 1103 7 0.11721405164438892 1103 8 0.30703138159442034 1103 9 0.4021684200338598 1103 10-11 0.33953805019329053 1103 12-13 0.3160181246737537 1103 14-15 0.5096009582582113 1103 16-17 0.35300067008960667 1103 18-19 0.3899035188752791 1103 20-21 0.5044178351749053 1103 22-23 0.39042290108677236 1103 24-25 0.32171037539988845 1103 26-27 0.4088535053979072 1103 28-29 0.432623572668426 1103 30-31 0.5025318371464422 1103 32-33 0.4869506727877351 1103 34-35 0.42141248886697724 1103 36-37 0.49233895481794576 1103 38-39 0.6711713326762165 1103 40-41 0.671983520237923 1103 42-43 0.4635169500801979 1103 44-45 0.4608206563215518 1103 46-47 0.5281031060768981 1103 48-49 0.5809936342938613 1103 50-51 0.39085005967340436 1103 52-53 0.5244599330066393 1103 54-55 0.5896263874728831 1103 56-57 0.6194664645034322 1103 58-59 0.41993101470946925 1103 60-61 0.4907245929877533 1103 62-63 0.46243619072713216 1103 64-65 0.5051694438367917 1103 66-67 0.34428289269339274 1103 68-69 0.34720174483322097 1103 70-71 0.3766906198711695 1103 72-73 0.25124153876722843 1103 74-75 0.2439288483186317 1103 76-77 0.3216573429261871 1103 78-79 0.2546909390408345 1103 80-81 0.20476518005607502 1103 82-83 0.09929178423836049 1103 84-85 0.01862732556368485 1103 86-87 -0.1213538264441425 1103 88-89 -0.05537732269822371 1103 90-91 -0.11913742990150666 1103 92-93 -0.13436363851623057 1103 94-95 -0.19351884548607856 1103 96-97 -0.31456741892587914 1103 98-99 -0.24961304638900472 1103 100-101 -0.40322914541035004 1104 1 0.26705376588040863 1104 2 0.3097285868307438 1104 3 0.42600754498636917 1104 4 0.3494541226119381 1104 5 0.3228757014970398 1104 6 0.24339238433282162 1104 7 0.31529490991997733 1104 8 0.36225379501712496 1104 9 0.395597247521593 1104 10-11 0.444647515485336 1104 12-13 0.4261825884359638 1104 14-15 0.4120330863051507 1104 16-17 0.567283129385963 1104 18-19 0.46365897369494036 1104 20-21 0.5089756882707306 1104 22-23 0.4502815043491424 1104 24-25 0.4254573021474357 1104 26-27 0.37230474891233456 1104 28-29 0.3385830241810197 1104 30-31 0.3896429472931118 1104 32-33 0.3894804505153573 1104 34-35 0.33116204998592025 1104 36-37 0.6418126230390513 1104 38-39 0.8177053115101103 1104 40-41 0.548729876277342 1104 42-43 0.4268774878912396 1104 44-45 0.6385124782692913 1104 46-47 0.5624241478470964 1104 48-49 0.531006111753058 1104 50-51 0.576916101237444 1104 52-53 0.526223269240532 1104 54-55 0.4901868966400258 1104 56-57 0.428417468233647 1104 58-59 0.511436577486279 1104 60-61 0.6163211782869169 1104 62-63 0.4539664914230528 1104 64-65 0.49523651831746207 1104 66-67 0.5141241143125939 1104 68-69 0.5497100396311012 1104 70-71 0.4815689809189365 1104 72-73 0.41851630977872745 1104 74-75 0.31560848792621243 1104 76-77 0.27258599138950856 1104 78-79 0.2546629461323704 1104 80-81 0.32957622792395114 1104 82-83 0.1524305704745288 1104 84-85 0.18584979974619387 1104 86-87 -0.08603371994089315 1104 88-89 -0.10808276135143657 1104 90-91 0.03529467210552184 1104 92-93 0.16533429847151382 1104 94-95 -0.03761809228154078 1104 96-97 -0.09302806935955132 1104 98-99 -0.17797051366008887 1104 100-101 0.007195066502475811 1105 1 0.2246340724321918 1105 2 0.3234026498220999 1105 3 0.31345077787172926 1105 4 0.34481367507746796 1105 5 0.34114816616290966 1105 6 0.2478850469139644 1105 7 0.35957211452804216 1105 8 0.21949186492066275 1105 9 0.258032768530164 1105 10-11 0.37069571669267276 1105 12-13 0.41631117799718 1105 14-15 0.5270768035921236 1105 16-17 0.5353130810249027 1105 18-19 0.4088243034281476 1105 20-21 0.4947741786994655 1105 22-23 0.4777932345196234 1105 24-25 0.34704593627726865 1105 26-27 0.38958122625191294 1105 28-29 0.3010731215484057 1105 30-31 0.4117757214581417 1105 32-33 0.14112721200584843 1105 34-35 0.26202387771528635 1105 36-37 0.45794732631808444 1105 38-39 0.773073214866244 1105 40-41 0.5606954619491091 1105 42-43 0.3617107760564906 1105 44-45 0.48077347981833185 1105 46-47 0.4230365020863758 1105 48-49 0.4912996251592361 1105 50-51 0.4038543009003277 1105 52-53 0.4079667903273574 1105 54-55 0.6102794474874074 1105 56-57 0.5209533289810935 1105 58-59 0.5861094003329228 1105 60-61 0.5429738407681484 1105 62-63 0.5919431031012223 1105 64-65 0.4656472647397152 1105 66-67 0.5022159774395831 1105 68-69 0.48767761769381224 1105 70-71 0.27477370387626365 1105 72-73 0.6456684437726388 1105 74-75 0.7111166163067963 1105 76-77 0.6278982776477129 1105 78-79 0.5860940165242177 1105 80-81 0.5916722182059466 1105 82-83 0.6526268538702844 1105 84-85 0.5945484637285858 1105 86-87 0.6036744942798506 1105 88-89 0.6565830219358766 1105 90-91 0.7214733237156068 1105 92-93 0.7971116435793526 1105 94-95 0.67395625874477 1105 96-97 0.3692172461696508 1105 98-99 0.3388024540990635 1105 100-101 0.3626189199930536 1106 1 0.22162516626089612 1106 2 0.31403696686842864 1106 3 0.13111774769673445 1106 4 0.21988021475182506 1106 5 0.2981128112170097 1106 6 0.24162614014589678 1106 7 0.26720430469186596 1106 8 0.22131916969981802 1106 9 0.366759193195314 1106 10-11 0.4163168690285417 1106 12-13 0.4621382154241047 1106 14-15 0.464200690320709 1106 16-17 0.37605947117297234 1106 18-19 0.4616215955319447 1106 20-21 0.5015337741382453 1106 22-23 0.4632386505029942 1106 24-25 0.4927260493290717 1106 26-27 0.5073182566847052 1106 28-29 0.6035714326938901 1106 30-31 0.5223318379759689 1106 32-33 0.5940881379994067 1106 34-35 0.7502365086761102 1106 36-37 0.5779383509216487 1106 38-39 0.7339371445144067 1106 40-41 0.6834948785483377 1106 42-43 0.6246564725210035 1106 44-45 0.7368933395538875 1106 46-47 0.5485082474906591 1106 48-49 0.608807496534169 1106 50-51 0.530125715933643 1106 52-53 0.615664863720319 1106 54-55 0.7064164464108416 1106 56-57 0.671886197312503 1106 58-59 0.6820124660514395 1106 60-61 0.7828347459941654 1106 62-63 0.7337722697977327 1106 64-65 0.646384536266936 1106 66-67 0.47285079577135747 1106 68-69 0.7070980123725477 1106 70-71 0.7568205239362698 1106 72-73 0.645231609318575 1106 74-75 0.5258343567984802 1106 76-77 0.6962986758115761 1106 78-79 0.706082057285883 1106 80-81 0.6356283867619794 1106 82-83 0.6635739730306085 1106 84-85 0.892443900360135 1106 86-87 0.6732606712145142 1106 88-89 0.5894746273144271 1106 90-91 0.6700623245582449 1106 92-93 0.6895041596264555 1106 94-95 0.5755163306434277 1106 96-97 0.6564818508822121 1106 98-99 0.7778986603231175 1106 100-101 0.5708385689500197 1107 1 -0.05633499356377669 1107 2 0.020701008454590664 1107 3 -0.07251524162895606 1107 4 -0.04826641736145376 1107 5 -0.0021902940090328116 1107 6 -0.02465317452494986 1107 7 0.03634831477705802 1107 8 -0.13554755252087602 1107 9 -0.012137601459286884 1107 10-11 0.09123918496003824 1107 12-13 -0.04394296671679854 1107 14-15 0.05544894662624955 1107 16-17 -0.005220499767993658 1107 18-19 -0.012474048971107266 1107 20-21 -0.03716746728064635 1107 22-23 0.08632287590583587 1107 24-25 -0.08414378635920627 1107 26-27 -0.07551867697706172 1107 28-29 -0.026678346768825634 1107 30-31 -0.040011153211693795 1107 32-33 0.05763843728911411 1107 34-35 0.04780617251317665 1107 36-37 0.025983123806405217 1107 38-39 0.09824490162731081 1107 40-41 0.11000368935634697 1107 42-43 0.1444590420474796 1107 44-45 0.1966338381360373 1107 46-47 -0.011358468625189744 1107 48-49 0.14074190615050952 1107 50-51 0.19256134804387415 1107 52-53 0.19379076990726674 1107 54-55 0.029590565558969217 1107 56-57 0.13236194736821005 1107 58-59 0.2484632503705555 1107 60-61 0.027817331942664225 1107 62-63 0.08609165052292411 1107 64-65 0.21296619305903164 1107 66-67 0.015526668534313615 1107 68-69 0.19356167790066792 1107 70-71 0.11059857948419705 1107 72-73 0.06443025770735389 1107 74-75 0.11009257314784904 1107 76-77 -0.11871785303836546 1107 78-79 0.13883344689972432 1107 80-81 -0.05496237844898744 1107 82-83 0.22183664285958216 1107 84-85 0.07777267643161423 1107 86-87 0.16684524917177157 1107 88-89 0.10476572127094386 1107 90-91 0.14009138358897744 1107 92-93 0.4081466572379391 1107 94-95 0.14652513992522742 1107 96-97 0.2926865186416414 1107 98-99 0.3054978715779981 1107 100-101 0.1915632743152571 1108 1 -0.07456422651436512 1108 2 0.09524393947539167 1108 3 0.08583028170772877 1108 4 0.0577944722667354 1108 5 0.11196727819147156 1108 6 0.06453198426450513 1108 7 0.11263328087213154 1108 8 0.060430644716120696 1108 9 0.18030823974078203 1108 10-11 0.08250973266424921 1108 12-13 0.0601819956651255 1108 14-15 0.1279960609268045 1108 16-17 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1624.0 5 1849.0 6 2027.0 7 2460.0 8 3093.0 9 3871.0 10 4676.0 11 5580.0 12 7019.0 13 9612.0 14 8781.0 15 9792.0 16 12358.0 17 16195.0 18 14091.0 19 10011.0 20 9957.0 21 10843.0 22 11603.0 23 12879.0 24 14684.0 25 16434.0 26 19051.0 27 22149.0 28 26123.0 29 30951.0 30 37140.0 31 45534.0 32 56611.0 33 73298.0 34 99827.0 35 145967.0 36 226495.0 37 342155.0 38 295056.0 39 43851.0 40 10.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.53231755953441 19.387093988864486 16.360422982797985 21.720165468803117 2 15.229626450517397 20.475871576278635 39.43520615415403 24.859295819049933 3 18.88637502192852 25.34080379841078 29.92846028964329 25.844360890017416 4 13.034312212437182 17.36143933328832 37.2694071744813 32.334841279793196 5 14.060627253324972 37.50473273606405 33.315206250828986 15.119433759781991 6 30.923480401518983 35.975724742242576 17.70499645438569 15.395798401852756 7 28.036183183845427 33.37828213628525 20.513464757366844 18.07206992250248 8 27.188182027435957 33.259679893755866 19.843526109712492 19.70861196909568 9 27.227673256111025 16.6710049987226 20.183668107956983 35.91765363720939 10-11 25.49247560480002 26.690462110204756 26.29361364076613 21.5234486442291 12-13 26.632494556041742 24.085192072006066 26.84766196001779 22.434651411934407 14-15 23.700154214013143 24.9625762145295 25.965253495120976 25.37201607633639 16-17 22.32929478921764 28.34276490443304 26.460256652812543 22.86768365353678 18-19 22.352504148575022 26.841973335839953 28.914272423695746 21.89125009188928 20-21 23.931623674114263 25.429378187232572 28.653744295896903 21.985253842756265 22-23 23.300597891605747 25.548107480078063 28.668093475138367 22.483201153177824 24-25 22.977721434918823 25.809693389112358 28.768768243921855 22.443816932046957 26-27 22.572607230890693 25.884545613432415 29.0016091413694 22.541238014307496 28-29 22.50059359734454 26.21377097628383 28.873237739050992 22.41239768732064 30-31 22.7101652287703 25.859325495886683 28.668157342310728 22.762351933032285 32-33 22.55755104475705 25.944496349458905 28.557556467918833 22.940396137865207 34-35 23.187184243698304 25.94614854643083 28.32876287356228 22.537904336308586 36-37 23.155707862167485 25.565323482686335 28.660379285360776 22.6185893697854 38-39 22.829671640817235 25.907106363775313 28.48894846887949 22.774273526527963 40-41 22.88175903507482 25.824155081920967 28.67150945327933 22.62257642972488 42-43 22.72631432042721 25.865283477796456 28.183057245338805 23.225344956437525 44-45 22.857579631073634 26.00532993673986 28.115914794728553 23.021175637457958 46-47 23.284109339385722 26.265375700049066 27.535253226208 22.915261734357212 48-49 23.080042351473722 26.252927980738782 27.538489802529003 23.128539865258496 50-51 23.114951582683794 26.52285142022012 27.461104045940626 22.901092951155448 52-53 23.294577625225855 26.564711356590788 27.1277367190191 23.012974299164256 54-55 22.994411044983533 26.501374580828376 27.275399534757256 23.228814839430832 56-57 23.155779234449152 26.649605831893897 27.116397761694188 23.078217171962763 58-59 23.192885424911825 26.220741361289434 27.27948377775771 23.306889436041033 60-61 23.037035337560066 26.334569635744614 27.686784202941162 22.941610823754154 62-63 22.923048713551534 26.433628877296446 27.734078703042208 22.90924370610982 64-65 22.882089577945358 26.493985561964468 27.49614513334983 23.127779726740343 66-67 22.784041667220666 26.47204759481553 27.289267596338522 23.45464314162528 68-69 22.947286727526752 26.398372148335657 27.4253596586903 23.22898146544729 70-71 23.177637710750307 26.366741495340435 27.172237715827535 23.283383078081727 72-73 22.985296157287394 26.24177080667322 27.34130313980722 23.431629896232163 74-75 22.757778967687504 26.898614453535302 26.964519470535837 23.37908710824136 76-77 22.988172715157518 26.425357466670334 27.0993377723543 23.48713204581785 78-79 23.0180977242068 26.481748526392035 27.181867115812263 23.318286633588897 80-81 22.977651169274367 26.682037296078132 26.97790127174691 23.36241026290059 82-83 23.0297998940228 26.511459011173528 27.155388312177166 23.303352782626508 84-85 22.81611343222076 26.641417612725714 27.05115078464718 23.49131817040634 86-87 23.018199427882468 26.647964644598645 26.999216653943208 23.33461927357568 88-89 22.927053005224742 26.446654556573595 26.894558797402496 23.73173364079917 90-91 22.771124900418393 26.469334909714693 27.10269810241885 23.656842087448062 92-93 22.945222452569038 26.34414418770512 27.143806927730868 23.566826431994972 94-95 22.780846305109968 26.29554828994365 27.218171537813195 23.705433867133184 96-97 22.653585048900425 26.215831582230415 27.358669901207875 23.77191346766129 98-99 22.665489348534518 26.280907622832718 27.007212584491313 24.04639044414145 100-101 22.80556367076106 25.698819899739362 27.505390527354756 23.99022590214482 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1120.0 1 644.0 2 183.5 3 217.5 4 216.5 5 214.0 6 386.0 7 814.5 8 1443.5 9 1930.0 10 1857.5 11 1664.0 12 1743.0 13 1847.5 14 1991.0 15 2191.5 16 2344.5 17 2492.0 18 2611.5 19 2640.5 20 2752.0 21 3094.0 22 3594.0 23 4261.0 24 5066.0 25 6034.0 26 7574.5 27 9438.5 28 11263.5 29 13514.5 30 16228.5 31 19248.5 32 22820.0 33 25741.0 34 28196.0 35 30831.5 36 33866.5 37 37341.5 38 40191.0 39 42067.5 40 43658.5 41 46210.0 42 48980.0 43 51369.5 44 53849.5 45 56785.0 46 58695.5 47 62861.5 48 72201.0 49 72759.0 50 64943.0 51 61113.5 52 59929.0 53 59977.0 54 60179.0 55 57434.5 56 52775.0 57 48286.5 58 44209.5 59 39351.0 60 33012.0 61 27928.0 62 23044.5 63 17038.0 64 12241.5 65 9433.5 66 7347.5 67 5831.0 68 4634.0 69 3532.0 70 2668.0 71 1860.0 72 1384.0 73 1138.0 74 874.0 75 684.5 76 484.0 77 288.5 78 188.0 79 129.0 80 86.0 81 61.5 82 42.0 83 30.5 84 24.5 85 17.5 86 16.0 87 12.5 88 12.0 89 11.5 90 6.5 91 3.5 92 2.5 93 2.0 94 3.5 95 3.5 96 1.5 97 2.0 98 2.5 99 1.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.278425570835644 2 0.1077815250423449 3 0.11849946440409763 4 0.1203660718210321 5 0.12686909120906184 6 0.05840674820730422 7 0.06629466987241438 8 0.07183427898073601 9 0.06882362185664816 10-11 0.064157103314312 12-13 0.06798063786190357 14-15 0.06385603760190321 16-17 0.06581296473256032 18-19 0.06990745842131979 20-21 0.07321918125781642 22-23 0.08143827520657625 24-25 0.08920577058672288 26-27 0.09013907429519011 28-29 0.08387690747708738 30-31 0.08071571749679515 32-33 0.07336971411402081 34-35 0.23672796966702736 36-37 0.35634137720703746 38-39 0.3297271682301009 40-41 0.31175354519929643 42-43 0.334664645913605 44-45 0.3477610044033871 46-47 0.35423391722017594 48-49 0.3638680200172571 50-51 0.35739510720046824 52-53 0.358689689763826 54-55 0.34423853556820433 56-57 0.3202435982892242 58-59 0.3085020355052816 60-61 0.26999573088819806 62-63 0.33541731019462695 64-65 0.3637174871610527 66-67 0.3692872028406152 68-69 0.3803363144860176 70-71 0.38060727362718555 72-73 0.3651023894381331 74-75 0.3680829399909801 76-77 0.3505910221000297 78-79 0.3297271682301009 80-81 0.3278906673844073 82-83 0.3428536332911239 84-85 0.3484835621131682 86-87 0.34246224786499246 88-89 0.35820798462397196 90-91 0.38205238904674765 92-93 0.37675363250835303 94-95 0.37606118136981287 96-97 0.3813599379082075 98-99 0.3708527445451409 100-101 0.3480620701157959 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1660767.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.95237914511563 #Duplication Level Percentage of deduplicated Percentage of total 1 74.91595662640577 41.91726009179694 2 14.274167270795918 15.973472382327472 3 4.662553835830752 7.8264294002074655 4 2.089396620293536 4.676268475327485 5 1.1217517855750343 3.138234060660239 6 0.6786978754631984 2.2784856511740808 7 0.4385484085535207 1.7176478780211564 8 0.31179586309748963 1.3956576278327448 9 0.22109395229211276 1.113365938080636 >10 1.1825942919616268 11.849254770426821 >50 0.07271483050040603 2.744749779027684 >100 0.02749432132220792 2.9792530948774614 >500 0.002156211938979367 0.8428272828580111 >1k 8.624847755917465E-4 0.8446972832708797 >5k 2.1562119389793662E-4 0.7023962841109849 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6398 0.3852436855982808 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5246 0.3158781454592968 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3211 0.19334440050892146 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2709 0.1631174029830795 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2142 0.12897655119592333 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 9.031971372263538E-5 0.0 12-13 0.0 0.0 0.0 1.2042628496351385E-4 0.0 14-15 0.0 0.0 0.0 1.2042628496351385E-4 0.0 16-17 0.0 0.0 0.0 1.8063942744527076E-4 0.0 18-19 0.0 0.0 0.0 2.408525699270277E-4 0.0 20-21 0.0 0.0 0.0 2.408525699270277E-4 0.0 22-23 0.0 0.0 0.0 2.408525699270277E-4 0.0 24-25 0.0 0.0 0.0 2.709591411679062E-4 0.0 26-27 0.0 0.0 0.0 5.720248535766907E-4 0.0 28-29 0.0 0.0 0.0 0.0012343694208760171 0.0 30-31 0.0 0.0 0.0 0.002679484840438183 0.0 32-33 0.0 0.0 0.0 0.006141740533139206 0.0 34-35 0.0 0.0 0.0 0.011711456212701721 0.0 36-37 0.0 0.0 0.0 0.02155630500846898 0.0 38-39 0.0 0.0 0.0 0.03799449290598862 0.0 40-41 0.0 0.0 0.0 0.05608854222175658 0.0 42-43 0.0 0.0 0.0 0.07860825750993367 0.0 44-45 0.0 0.0 0.0 0.10754067247241786 0.0 46-47 0.0 0.0 0.0 0.1447825010973845 6.0213142481756924E-5 48-49 0.0 0.0 0.0 0.18515541313140255 6.0213142481756924E-5 50-51 0.0 0.0 0.0 0.2323023036946182 6.0213142481756924E-5 52-53 0.0 0.0 0.0 0.2826103842381261 6.0213142481756924E-5 54-55 0.0 0.0 0.0 0.3424321412937516 6.0213142481756924E-5 56-57 0.0 0.0 0.0 0.4138750348483562 6.0213142481756924E-5 58-59 0.0 0.0 0.0 0.4939886209203338 6.0213142481756924E-5 60-61 0.0 0.0 0.0 0.5948757411485175 6.0213142481756924E-5 62-63 0.0 0.0 0.0 0.6994057564968474 6.0213142481756924E-5 64-65 0.0 0.0 0.0 0.8107398569456161 6.0213142481756924E-5 66-67 0.0 0.0 0.0 0.940800244706211 6.0213142481756924E-5 68-69 0.0 0.0 0.0 1.0911223549119171 6.0213142481756924E-5 70-71 6.0213142481756924E-5 0.0 0.0 1.26257927812872 6.0213142481756924E-5 72-73 6.0213142481756924E-5 0.0 0.0 1.4439713698550127 1.2042628496351385E-4 74-75 6.0213142481756924E-5 0.0 0.0 1.6384899266423285 1.2042628496351385E-4 76-77 6.0213142481756924E-5 0.0 0.0 1.8507111473192808 1.2042628496351385E-4 78-79 6.0213142481756924E-5 0.0 0.0 2.1021913368943386 1.2042628496351385E-4 80-81 6.0213142481756924E-5 0.0 0.0 2.3792018988816612 1.2042628496351385E-4 82-83 6.0213142481756924E-5 0.0 0.0 2.6709646807770144 1.2042628496351385E-4 84-85 6.0213142481756924E-5 0.0 0.0 2.9793462899973324 1.2042628496351385E-4 86-87 6.0213142481756924E-5 0.0 0.0 3.306935891669331 1.2042628496351385E-4 88-89 6.0213142481756924E-5 0.0 0.0 3.670141567119289 1.2042628496351385E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATACG 55 0.0025813726 34.54158 1 GTATCAA 12880 0.0 27.287312 1 GGTATCA 9305 0.0 24.959583 1 GAGTACT 5885 0.0 23.11826 12-13 TATCAAC 15075 0.0 23.060875 2 ATCAACG 15210 0.0 22.856192 3 TCAACGC 15300 0.0 22.75274 4 CAACGCA 15410 0.0 22.559553 5 AACGCAG 15680 0.0 22.189295 6 GTACTTT 6095 0.0 22.049515 14-15 GAGTACG 195 4.2283318E-7 21.920618 1 GTACATG 9890 0.0 21.706364 1 TACATGG 9960 0.0 21.189943 2 AGTACTT 6035 0.0 20.933393 12-13 ACGCAGA 17170 0.0 20.125689 7 CGCAGAG 17235 0.0 19.99478 8 CGTAAGA 215 1.095339E-6 19.853321 2 ACATGGG 10345 0.0 19.62196 3 AGAGTAC 13145 0.0 19.274971 10-11 GCAGAGT 18035 0.0 18.608469 9 >>END_MODULE