FastQCFastQC Report
Fri 27 May 2016
SRR939304_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939304_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1660767
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64750.3898800975693761No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58120.34995878410397124No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT34230.20610958671505394No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31280.18834670968293565No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT19370.11663285698716315No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA106900.024.9218311
GTATCAA140000.023.9229241
GTACTTT64400.023.32297114-15
GAGTACT66400.023.15650412-13
GTACATG99450.023.1053181
TACATGG97750.022.9136942
ACATGGG100850.021.5035513
AGTACTT67550.020.61953212-13
TATCAAC166050.020.1760312
ATCAACG165500.020.157063
TCAACGC165600.020.144894
CAACGCA166450.020.0420175
AACGCAG169500.019.681386
ACTTTTT76300.019.59185216-17
TACTTTT72800.018.61104614-15
AGAGTAC137500.018.43011710-11
GTATTAG6050.018.0859811
ACGCAGA184150.018.0640957
CGCAGAG185250.017.9312158
CATGGGA56200.017.562934
Produced by FastQC (version 0.11.5)