##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939304_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1660767 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.26678275760537 33.0 31.0 34.0 31.0 34.0 2 32.46998404953856 34.0 31.0 34.0 31.0 34.0 3 32.54619642610914 34.0 31.0 34.0 31.0 34.0 4 36.0941426461388 37.0 35.0 37.0 35.0 37.0 5 36.03340564931745 37.0 35.0 37.0 35.0 37.0 6 36.072782033843396 37.0 35.0 37.0 35.0 37.0 7 36.01983962831631 37.0 35.0 37.0 35.0 37.0 8 36.00042510478592 37.0 35.0 37.0 35.0 37.0 9 37.69804614373961 39.0 37.0 39.0 35.0 39.0 10-11 37.61530967318113 39.0 37.0 39.0 35.0 39.0 12-13 37.53647802491258 39.0 37.0 39.0 35.0 39.0 14-15 38.802913653751546 40.0 38.0 41.0 34.5 41.0 16-17 38.74265234075581 40.0 38.0 41.0 34.0 41.0 18-19 38.87835259250696 40.0 38.0 41.0 35.0 41.0 20-21 38.88644975484219 40.0 38.0 41.0 35.0 41.0 22-23 38.82839525351841 40.0 38.0 41.0 34.5 41.0 24-25 38.76240466001552 40.0 38.0 41.0 34.0 41.0 26-27 38.59034199258535 40.0 38.0 41.0 34.0 41.0 28-29 38.4476275118665 40.0 38.0 41.0 34.0 41.0 30-31 38.25299063625421 40.0 38.0 41.0 34.0 41.0 32-33 38.045144803575695 40.0 37.0 41.0 33.0 41.0 34-35 38.007877685430884 40.0 37.0 41.0 33.0 41.0 36-37 37.92068604446018 40.0 37.0 41.0 33.0 41.0 38-39 37.82318260177376 40.0 37.0 41.0 33.0 41.0 40-41 37.65280319274167 40.0 37.0 41.0 33.0 41.0 42-43 37.46799761796808 40.0 37.0 41.0 32.5 41.0 44-45 37.22007211125944 40.0 36.5 41.0 31.5 41.0 46-47 36.89722911160928 40.0 36.0 41.0 31.0 41.0 48-49 36.538885346349005 39.5 36.0 41.0 30.0 41.0 50-51 36.26981117760649 39.0 35.0 41.0 30.0 41.0 52-53 36.02612196653715 39.0 35.0 41.0 29.0 41.0 54-55 35.74036695093291 39.0 35.0 40.0 28.5 41.0 56-57 35.47790358310347 38.0 35.0 40.0 28.0 41.0 58-59 35.16515170400183 38.0 34.0 40.0 28.0 41.0 60-61 34.81666603442867 37.5 34.0 40.0 27.0 41.0 62-63 34.417892455714735 37.0 34.0 40.0 26.0 41.0 64-65 33.98473115132948 36.5 33.0 39.0 26.0 41.0 66-67 33.59484984949725 36.0 33.0 39.0 26.0 40.0 68-69 33.195086065655204 35.5 33.0 39.0 25.0 40.0 70-71 32.78382217373057 35.0 32.0 38.0 24.0 40.0 72-73 32.29113746841068 35.0 32.0 37.0 22.5 39.5 74-75 31.795141642385715 35.0 31.0 37.0 21.5 39.0 76-77 29.824598212753504 33.0 28.5 35.0 18.0 37.0 78-79 30.826469336156126 34.0 30.5 36.0 19.5 37.5 80-81 30.889285191721655 34.0 31.0 35.0 19.5 37.0 82-83 30.59369225183304 34.0 31.0 35.0 18.0 37.0 84-85 30.229338612821664 34.0 30.5 35.0 16.5 36.0 86-87 29.716512611341628 34.0 30.0 35.0 8.5 36.0 88-89 29.21668843371767 34.0 29.0 35.0 3.5 35.5 90-91 28.804452099541958 34.0 29.0 35.0 2.0 35.0 92-93 28.453446509955942 34.0 29.0 35.0 2.0 35.0 94-95 28.05018554679856 33.5 29.0 35.0 2.0 35.0 96-97 27.491007468236063 33.0 27.0 35.0 2.0 35.0 98-99 26.725607505447783 33.0 26.0 35.0 2.0 35.0 100-101 23.33471673028185 29.0 12.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.17344373782347589 1101 2 0.16053046992083608 1101 3 0.2000362329915717 1101 4 0.16658296703094777 1101 5 0.19819935228503738 1101 6 0.16123627727427703 1101 7 0.18019831343690385 1101 8 0.19916087080022038 1101 9 0.26990389510782364 1101 10-11 0.20782493807874403 1101 12-13 0.30496106043293736 1101 14-15 0.31518416363007873 1101 16-17 0.294980666733899 1101 18-19 0.21745873049371056 1101 20-21 0.24220653661429736 1101 22-23 0.39097489475003755 1101 24-25 0.43558363799781574 1101 26-27 0.35609573281136164 1101 28-29 0.41966383135633123 1101 30-31 0.33638537759028964 1101 32-33 0.35274943444488827 1101 34-35 0.403493778959934 1101 36-37 0.5792954188655983 1101 38-39 0.606920029183577 1101 40-41 0.6309595264143226 1101 42-43 0.7259975787460888 1101 44-45 0.8264213304431962 1101 46-47 0.7236652201358638 1101 48-49 0.7008820050707598 1101 50-51 0.7492518969487207 1101 52-53 0.6107662909877192 1101 54-55 0.6941019498597143 1101 56-57 0.7006725801231326 1101 58-59 0.652038436355447 1101 60-61 0.7550141118108797 1101 62-63 0.7562168409787091 1101 64-65 0.6906112708558751 1101 66-67 0.8143869132603356 1101 68-69 0.8457960068177357 1101 70-71 0.8009893462857463 1101 72-73 0.7328822790175664 1101 74-75 0.6384735962620773 1101 76-77 0.7570637907161597 1101 78-79 0.9779213461527156 1101 80-81 0.9982425610052523 1101 82-83 1.0316792768566785 1101 84-85 0.9048559041483166 1101 86-87 0.899719148040159 1101 88-89 0.7812656980416968 1101 90-91 0.8963900001993466 1101 92-93 0.8969251382869139 1101 94-95 1.0679754359388482 1101 96-97 0.9034850492326179 1101 98-99 0.7723171160404227 1101 100-101 0.7978854568039644 1102 1 0.12451651267342356 1102 2 0.08745323013163642 1102 3 0.1109744192014972 1102 4 0.07378176662104607 1102 5 0.09023245505184008 1102 6 0.0594251405446613 1102 7 0.11001916689905755 1102 8 0.1126714292836013 1102 9 0.19233335272749486 1102 10-11 0.21992213285256668 1102 12-13 0.086970466083649 1102 14-15 0.1496472272371676 1102 16-17 0.2656335268568668 1102 18-19 0.273714677646403 1102 20-21 0.19424435135591267 1102 22-23 0.18959469785354344 1102 24-25 0.23328026001508562 1102 26-27 0.3106157975895769 1102 28-29 0.3313476990184583 1102 30-31 0.3615697393235706 1102 32-33 0.3081762112711175 1102 34-35 0.31399667934055486 1102 36-37 0.3716885531065586 1102 38-39 0.3953365769071837 1102 40-41 0.5094221416975913 1102 42-43 0.5573754425288442 1102 44-45 0.5479200586674722 1102 46-47 0.5493116298855298 1102 48-49 0.3296701125952808 1102 50-51 0.3602019774640226 1102 52-53 0.43665356042118475 1102 54-55 0.3653131561252678 1102 56-57 0.47363842516778476 1102 58-59 0.4244864216431026 1102 60-61 0.5089662235075636 1102 62-63 0.4557685171608128 1102 64-65 0.4302890724222692 1102 66-67 0.29650748491895484 1102 68-69 0.44119542674161494 1102 70-71 0.38411818906132567 1102 72-73 0.6640645729716042 1102 74-75 0.5460948724295527 1102 76-77 0.6097503025738682 1102 78-79 0.7957845341925349 1102 80-81 0.6969531725555385 1102 82-83 0.5751637136155701 1102 84-85 0.5449147809599104 1102 86-87 0.3807600827495712 1102 88-89 0.49119538439801147 1102 90-91 0.5380715347477292 1102 92-93 0.4575563599235686 1102 94-95 0.600814011916011 1102 96-97 0.29419170821373086 1102 98-99 0.3461713742017416 1102 100-101 0.36666038909791965 1103 1 0.11483998154530184 1103 2 0.0941054221004407 1103 3 0.09157621755481671 1103 4 0.1192569941235746 1103 5 0.06216794183502117 1103 6 0.012517640183546064 1103 7 0.0970433977238443 1103 8 0.02232719629212454 1103 9 0.10397886406651224 1103 10-11 0.15159486324402138 1103 12-13 0.17100466368584932 1103 14-15 0.27411119697572417 1103 16-17 0.131038923722393 1103 18-19 0.13107104410602233 1103 20-21 0.22431779314415934 1103 22-23 0.16325360656363586 1103 24-25 0.10258141197233073 1103 26-27 0.27117006416476386 1103 28-29 0.11289291063191342 1103 30-31 0.06501161784443354 1103 32-33 0.07016500767441869 1103 34-35 0.17700743154335186 1103 36-37 0.25363460250711256 1103 38-39 0.14724800439527286 1103 40-41 0.24604056940234642 1103 42-43 0.18228358386930665 1103 44-45 0.1917971260515472 1103 46-47 0.2609751969807306 1103 48-49 0.01715995623243316 1103 50-51 -0.03995691319032346 1103 52-53 -0.0612562601046065 1103 54-55 0.04575245267300687 1103 56-57 0.15561178389955188 1103 58-59 -0.03466581546508252 1103 60-61 -0.07324298853924205 1103 62-63 -0.1565509252774291 1103 64-65 -0.12421696722817188 1103 66-67 -0.06484281480660314 1103 68-69 -0.2465146971208796 1103 70-71 -0.0668518564391789 1103 72-73 -0.05620572169834048 1103 74-75 0.14162359010974868 1103 76-77 0.2380967112839656 1103 78-79 0.1850569323580693 1103 80-81 -0.011700725682217694 1103 82-83 0.0385956163311576 1103 84-85 0.04583043349494176 1103 86-87 -0.11138972585941076 1103 88-89 0.14751512475623585 1103 90-91 0.40622366352138073 1103 92-93 0.23027636931255913 1103 94-95 0.22024900216590382 1103 96-97 0.22298905943430114 1103 98-99 0.16427881497886077 1103 100-101 0.06902095352085524 1104 1 0.18313877220825248 1104 2 0.18676802509372692 1104 3 0.10007911775033307 1104 4 0.0026255175085054816 1104 5 0.0820186029233625 1104 6 0.02270619086698389 1104 7 0.10538398245013525 1104 8 0.12371360475857074 1104 9 0.050864381750869825 1104 10-11 0.23706046777149226 1104 12-13 0.1434064769319221 1104 14-15 0.24204629682793666 1104 16-17 0.1429007559341997 1104 18-19 0.13050185496727096 1104 20-21 0.15976245724010596 1104 22-23 0.1820544250849636 1104 24-25 0.07040584218644597 1104 26-27 0.16705575761854163 1104 28-29 0.11026635122077266 1104 30-31 0.1671755347684325 1104 32-33 0.1887611958648847 1104 34-35 0.11062474461473926 1104 36-37 0.2083451146037376 1104 38-39 0.11612661716220174 1104 40-41 0.29523322354236825 1104 42-43 0.4021138470243457 1104 44-45 0.3811556978490245 1104 46-47 0.39672105609177066 1104 48-49 0.41077496911133693 1104 50-51 0.2431145381660329 1104 52-53 0.31076233239459583 1104 54-55 0.3620890272524093 1104 56-57 0.39634341870710443 1104 58-59 0.47791905838237625 1104 60-61 0.3153263568390017 1104 62-63 0.4046847554818882 1104 64-65 0.4293911884731614 1104 66-67 0.2901519663604617 1104 68-69 0.33875257005516346 1104 70-71 0.21716679681982498 1104 72-73 0.20872936296392908 1104 74-75 0.14023956863549714 1104 76-77 0.2963488687994129 1104 78-79 0.27050778093379435 1104 80-81 0.30982408493164826 1104 82-83 0.30626510905191395 1104 84-85 0.3936431891472303 1104 86-87 0.13459611276689643 1104 88-89 0.4064599440066772 1104 90-91 0.3615691155417551 1104 92-93 0.21645177851896946 1104 94-95 0.2554313141197433 1104 96-97 0.3950677607369464 1104 98-99 0.6067230081863535 1104 100-101 0.17024699951168643 1105 1 0.09655760434078076 1105 2 0.11497742513260789 1105 3 0.06302172848184995 1105 4 0.08631051658640843 1105 5 0.0720970288595808 1105 6 0.08969357169715408 1105 7 0.09771123745078114 1105 8 0.16259894819622644 1105 9 0.0960274634876086 1105 10-11 0.01832187687396214 1105 12-13 0.1250130783817056 1105 14-15 0.14735610612707006 1105 16-17 0.1626188072209942 1105 18-19 0.1429166236380297 1105 20-21 0.0826942692626389 1105 22-23 0.14634375222214402 1105 24-25 0.2794287172157439 1105 26-27 0.1253021371994123 1105 28-29 0.11577664850165093 1105 30-31 0.20881779610301265 1105 32-33 0.2623630981694447 1105 34-35 0.2331813493740853 1105 36-37 0.31536806373365067 1105 38-39 0.2856409541760101 1105 40-41 0.46526626492756407 1105 42-43 0.43344425712967194 1105 44-45 0.6363259856633334 1105 46-47 0.704886611873512 1105 48-49 0.5535862302415282 1105 50-51 0.5508523501744094 1105 52-53 0.4869584226291792 1105 54-55 0.4500502151770007 1105 56-57 0.5186271416723471 1105 58-59 0.5170113007546959 1105 60-61 0.44875972601538905 1105 62-63 0.39821489674348953 1105 64-65 0.5031483690526315 1105 66-67 0.48442444601297296 1105 68-69 0.5379591938184021 1105 70-71 0.5611396060535938 1105 72-73 0.4776080581601647 1105 74-75 0.42503262182683343 1105 76-77 0.5517158563051296 1105 78-79 0.4674714037716079 1105 80-81 0.5609885679195195 1105 82-83 0.5594155304055519 1105 84-85 0.49822747839943204 1105 86-87 0.5482091468169656 1105 88-89 0.9024655873104166 1105 90-91 0.9625203927159731 1105 92-93 0.8525103080212197 1105 94-95 0.9099902301524949 1105 96-97 0.8417800450801955 1105 98-99 0.6760073002277345 1105 100-101 0.5955575248393217 1106 1 0.11925389936397579 1106 2 0.1325200284272512 1106 3 0.10508673804207547 1106 4 0.05449202010854037 1106 5 0.0898007596167858 1106 6 0.15112013457386553 1106 7 0.10138016945021633 1106 8 0.1072832935293846 1106 9 0.2341077312217692 1106 10-11 0.18893377929995125 1106 12-13 0.2409407070867431 1106 14-15 0.17319498343864836 1106 16-17 0.0785580132325947 1106 18-19 0.09088021121856826 1106 20-21 0.15350680521793691 1106 22-23 0.1664960236419617 1106 24-25 0.24277162271388875 1106 26-27 0.2843218138464323 1106 28-29 0.3023180892914894 1106 30-31 0.2947858618334749 1106 32-33 0.25350228126515617 1106 34-35 0.3492850247511754 1106 36-37 0.35356243358204864 1106 38-39 0.40626932740926947 1106 40-41 0.33297405370975497 1106 42-43 0.2065881284377582 1106 44-45 0.2565616853026569 1106 46-47 0.14803248832335925 1106 48-49 0.10650944047348077 1106 50-51 0.09546496394830939 1106 52-53 0.12115671205229717 1106 54-55 0.13935409679724842 1106 56-57 0.09627088527200556 1106 58-59 0.1745784431651103 1106 60-61 -0.061688494779367886 1106 62-63 0.17808912183975423 1106 64-65 0.2712907245690417 1106 66-67 0.16636360905725667 1106 68-69 0.11149418712265913 1106 70-71 0.12540201227144365 1106 72-73 0.3381862551080559 1106 74-75 0.27944321756877244 1106 76-77 0.23247520826241086 1106 78-79 0.27127246029034424 1106 80-81 0.36683295937093163 1106 82-83 0.4177081044585407 1106 84-85 0.5109732574566834 1106 86-87 0.501260781604266 1106 88-89 0.7368534103709798 1106 90-91 0.76967528752958 1106 92-93 0.6467738122928886 1106 94-95 0.6472229674335708 1106 96-97 0.5813365984324825 1106 98-99 0.7564486939922226 1106 100-101 0.6086655423031537 1107 1 -0.06582877540991916 1107 2 -0.013626750202156757 1107 3 -0.06157992862458883 1107 4 -0.06413414706025122 1107 5 -0.011880665496690312 1107 6 -0.07006121270292454 1107 7 9.082654426961767E-4 1107 8 0.043160360398729836 1107 9 -0.1297688716798575 1107 10-11 -0.08803587049600026 1107 12-13 0.015451251668856969 1107 14-15 0.04547031775486232 1107 16-17 0.011481864530942687 1107 18-19 0.012873349380207344 1107 20-21 -0.0017209205160995111 1107 22-23 -0.0036706231839858106 1107 24-25 -0.045144383867132376 1107 26-27 0.016967284514208814 1107 28-29 -0.06814868665178864 1107 30-31 -0.09072476211153457 1107 32-33 -0.06936926683029299 1107 34-35 -0.0224889497122831 1107 36-37 0.0067541957131354025 1107 38-39 -0.09984237892357584 1107 40-41 -0.09708929925064069 1107 42-43 -0.17246586580537837 1107 44-45 -0.24579821628735488 1107 46-47 -0.127805477056107 1107 48-49 0.1275093214291232 1107 50-51 0.011166684597007759 1107 52-53 -0.02378397944274724 1107 54-55 -0.07787742004141762 1107 56-57 -0.04021346039615281 1107 58-59 -0.08957142756493397 1107 60-61 -0.18863097804447904 1107 62-63 -0.08903189742407136 1107 64-65 -0.011766212206197224 1107 66-67 0.01701980287366922 1107 68-69 0.013718713921370806 1107 70-71 -0.07942790693101642 1107 72-73 -0.011087574365490127 1107 74-75 0.062124325230655586 1107 76-77 0.004791063443434496 1107 78-79 0.32110892693198423 1107 80-81 0.1034332206580153 1107 82-83 0.22860081497626084 1107 84-85 0.16300586339852075 1107 86-87 0.3194609836744178 1107 88-89 0.3717306573330603 1107 90-91 0.47616534122163046 1107 92-93 0.41241211893281715 1107 94-95 0.4216713189745853 1107 96-97 0.5166997142198966 1107 98-99 0.7623616115250123 1107 100-101 0.502359702109505 1108 1 -0.1333964542586017 1108 2 -0.03786252308330518 1108 3 -0.0037109757770679153 1108 4 -0.057341932092974446 1108 5 -0.04673746227823017 1108 6 -0.030239204219576266 1108 7 -0.009450367699834317 1108 8 -0.05717509551737976 1108 9 -0.08460135333496055 1108 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30.0 6 70.0 7 183.0 8 449.0 9 925.0 10 1423.0 11 2226.0 12 3171.0 13 4337.0 14 5582.0 15 7849.0 16 10446.0 17 15076.0 18 14643.0 19 9605.0 20 8673.0 21 9272.0 22 9759.0 23 10478.0 24 12333.0 25 14611.0 26 17617.0 27 21531.0 28 26295.0 29 32027.0 30 39333.0 31 47847.0 32 59592.0 33 76627.0 34 104425.0 35 150646.0 36 240544.0 37 383306.0 38 296810.0 39 23007.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.59347174559321 19.973459042641828 16.902472245500235 21.530596966264728 2 15.57834422288015 21.050032906482365 38.63672628369903 24.734896586938447 3 19.066009861708473 25.362979876165653 30.357178339887536 25.213831922238338 4 13.457456705245226 17.81658715521202 36.86242561418911 31.863530525353646 5 14.026350475412865 37.71678989286276 33.32375944367874 14.933100188045644 6 30.954552926449047 36.34236470257417 17.341083969033587 15.3619984019432 7 27.97653132558631 33.51933173045948 20.3841959769191 18.119940967035113 8 27.211764202925515 33.6509576599246 19.577520507091002 19.559757630058883 9 26.95591856052053 16.638336383128998 20.145149801266523 36.26059525508394 10-11 25.503186178434422 26.840580286096728 26.123562185423964 21.53267135004489 12-13 26.57862300972984 24.165671644487155 26.819656219084315 22.436049126698688 14-15 23.56144050893205 25.122137370106262 25.923380195184958 25.39304192577673 16-17 22.254031841666574 28.4487085190804 26.503802351770904 22.79345728748212 18-19 22.29569229157371 26.803398670614243 29.008945866578518 21.891963171233535 20-21 23.709075385047992 25.401185115070323 28.878795159104197 22.010944340777485 22-23 23.19828729737525 25.54121077791165 28.863651553770037 22.396850370943064 24-25 22.948928386058 25.777291924328892 28.969222635819857 22.30455705379325 26-27 22.477519138989347 25.90221502866784 29.148948725452296 22.471317106890517 28-29 22.455859250575188 26.18452799218674 29.054406789152242 22.305205968085833 30-31 22.61322629845126 25.862688745621753 28.78751203510185 22.73657292082514 32-33 22.54202425746658 25.910076488754896 28.651370119950602 22.896529133827926 34-35 23.123759000305594 25.86250137366153 28.552077954727128 22.461661671305755 36-37 23.120867697666938 25.540052541746117 28.78822737253929 22.550852388047655 38-39 22.777337016657757 25.93013225342677 28.67693882620379 22.615591903711678 40-41 22.86915158123399 25.807735750254672 28.803804007264254 22.519308661247084 42-43 22.69738347516313 25.836075188658114 28.345595408135 23.12094592804376 44-45 22.840112767784348 25.98774174202382 28.22765267426079 22.94449281593104 46-47 23.234698006459677 26.280856280603388 27.702671782384492 22.781773930552447 48-49 23.17293756439043 26.24139930526076 27.562234798740583 23.023428331608226 50-51 23.125158435831157 26.52747194519159 27.493441283455173 22.85392833552208 52-53 23.28749306796197 26.572571588910428 27.11644679837689 23.02348854475071 54-55 23.04239547148998 26.56766421779816 27.2139619826261 23.17597832808576 56-57 23.16550124129393 26.645519811027075 27.079807101176744 23.10917184650225 58-59 23.26783005214792 26.233105214400787 27.272812119055384 23.22625261439591 60-61 23.01257220482355 26.363544399531047 27.684231062661112 22.939652332984288 62-63 22.946466984777583 26.443338713460225 27.706509411609208 22.903684890152984 64-65 22.84047757466206 26.5126373876159 27.505130913948467 23.14175412377357 66-67 22.829662439101934 26.46650613842881 27.28552530246567 23.41830612000359 68-69 22.903873782631262 26.370552170881417 27.449790878119217 23.275783168368104 70-71 23.10483650024356 26.4036737242491 27.163954967795 23.32753480771234 72-73 22.989769184960927 26.264521151973756 27.308857895177347 23.43685176788797 74-75 22.713571500397105 26.960404439635422 26.897120426887096 23.428903633080377 76-77 22.920994282417436 26.414790880353216 27.15432282796437 23.50989200926497 78-79 22.9395213175599 26.460213865039467 27.272760116259537 23.3275047011411 80-81 22.97099974469274 26.58883608792204 27.04293979084073 23.39722437654449 82-83 23.027823891009394 26.488995747145744 27.16365390208259 23.319526459762265 84-85 22.747351073329373 26.644195121892476 27.112743690114264 23.495710114663886 86-87 22.882102729070567 26.63819879052212 26.984147075789167 23.495551404618148 88-89 22.813969782473325 26.463101558069503 26.948085398487702 23.774843260969465 90-91 22.661359911823997 26.485885313184344 27.11650810455431 23.73624667043735 92-93 22.73091288543185 26.4208344698564 27.178737294274274 23.66951535043748 94-95 22.586438250049 26.311435111438 27.22878261332945 23.873344025183552 96-97 22.43619965955489 26.27072310564938 27.369522636227718 23.92355459856801 98-99 22.40934459800803 26.375253120997712 26.981780105216448 24.233622175777818 100-101 22.453665164446857 25.822986943663594 27.58304985444956 24.140298037439987 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 188.0 1 151.0 2 133.0 3 177.5 4 162.0 5 160.5 6 339.5 7 767.0 8 1338.5 9 1745.5 10 1685.0 11 1496.0 12 1534.5 13 1633.0 14 1766.0 15 1895.0 16 2060.0 17 2169.0 18 2250.5 19 2438.5 20 2672.0 21 3057.5 22 3552.0 23 4168.0 24 4985.0 25 6098.0 26 7782.5 27 9685.5 28 11384.0 29 13545.5 30 16180.5 31 19421.5 32 23103.5 33 25886.5 34 28266.5 35 30610.0 36 33884.5 37 37448.5 38 40190.0 39 42691.0 40 44441.5 41 46525.0 42 49066.5 43 51425.0 44 54397.5 45 57337.5 46 59002.5 47 63528.0 48 72832.5 49 72902.5 50 64990.0 51 61271.5 52 60163.0 53 60581.5 54 60779.0 55 57629.5 56 53060.5 57 48540.5 58 44074.0 59 39351.0 60 33103.0 61 27583.0 62 22549.5 63 16695.5 64 11971.0 65 9145.5 66 7027.5 67 5578.0 68 4371.5 69 3330.5 70 2501.0 71 1720.0 72 1274.5 73 1033.5 74 798.5 75 596.5 76 395.5 77 220.5 78 135.5 79 91.0 80 54.5 81 34.5 82 20.5 83 12.5 84 6.5 85 3.5 86 3.0 87 1.5 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2684904023261541 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.002077353415620614 16-17 0.001113943135912503 18-19 0.0 20-21 0.0 22-23 0.0 24-25 6.0213142481756924E-5 26-27 0.0012644759921168955 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.003582681977664537 36-37 0.022459502145695334 38-39 0.007797601951387522 40-41 0.018515541313140253 42-43 0.00547939596583988 44-45 5.419182823358122E-4 46-47 1.8063942744527076E-4 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 6.322379960584478E-4 60-61 0.002408525699270277 62-63 0.0015354351332848015 64-65 3.0106571240878462E-5 66-67 0.0 68-69 7.8277085226284E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.22718418658366885 78-79 0.0 80-81 0.0013849022770804093 82-83 0.0 84-85 0.0 86-87 0.1026031947889138 88-89 0.353240400369227 90-91 0.3549564749299571 92-93 0.0 94-95 3.0106571240878462E-5 96-97 0.0 98-99 0.0 100-101 1.505328562043923E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1660767.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.34172398888042 #Duplication Level Percentage of deduplicated Percentage of total 1 73.53285068845548 39.95901876227604 2 14.887068957870536 16.179779846240606 3 5.049328913215204 8.231677143930426 4 2.2078667764677196 4.799171478841119 5 1.1911335897459012 3.236412638392805 6 0.7418889607481393 2.4189315081223635 7 0.4682713026785306 1.7812668917449135 8 0.3198088787520536 1.3903172654669937 9 0.23768343993583724 1.1624515100748813 >10 1.2501308030153697 12.195492853760161 >50 0.07992053795921658 2.9219428818994913 >100 0.03060942038057258 3.20181333851993 >500 0.0021069962816938187 0.7750899143037113 >1k 0.0011089454114177994 1.006194705265827 >5k 2.217890822835599E-4 0.7404392611607604 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6475 0.3898800975693761 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5812 0.34995878410397124 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3423 0.20610958671505394 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3128 0.18834670968293565 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1937 0.11663285698716315 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 6.0213142481756924E-5 0.0 10-11 0.0 0.0 0.0 1.505328562043923E-4 0.0 12-13 0.0 0.0 0.0 1.8063942744527076E-4 0.0 14-15 0.0 0.0 0.0 1.8063942744527076E-4 0.0 16-17 0.0 0.0 0.0 2.1074599868614922E-4 0.0 18-19 0.0 0.0 0.0 2.408525699270277E-4 0.0 20-21 0.0 0.0 0.0 2.408525699270277E-4 0.0 22-23 0.0 0.0 0.0 2.408525699270277E-4 0.0 24-25 0.0 0.0 0.0 2.709591411679062E-4 0.0 26-27 0.0 0.0 0.0 5.118117110949338E-4 0.0 28-29 0.0 0.0 0.0 0.001113943135912503 0.0 30-31 0.0 0.0 0.0 0.002619271697956426 0.0 32-33 0.0 0.0 0.0 0.006081527390657449 0.0 34-35 0.0 0.0 0.0 0.011741562783942599 0.0 36-37 0.0 0.0 0.0 0.02167673129343249 0.0 38-39 0.0 0.0 0.0 0.03826545204715652 0.0 40-41 0.0 0.0 0.0 0.056449821076647116 0.0 42-43 0.0 0.0 0.0 0.07890932322234245 0.0 44-45 0.0 0.0 0.0 0.10826323018219895 0.0 46-47 0.0 0.0 0.0 0.14625772308818757 0.0 48-49 0.0 0.0 0.0 0.1869015942633735 0.0 50-51 0.0 0.0 0.0 0.2342893373965162 0.0 52-53 0.0 0.0 0.0 0.2851393362223599 0.0 54-55 0.0 0.0 0.0 0.34550301156032126 0.0 56-57 0.0 0.0 0.0 0.4176985693959478 0.0 58-59 0.0 0.0 0.0 0.49802290146661155 0.0 60-61 0.0 0.0 0.0 0.59872938226735 0.0 62-63 0.0 0.0 0.0 0.7046141933215195 0.0 64-65 0.0 0.0 0.0 0.81670095805131 0.0 66-67 0.0 0.0 0.0 0.9468516655256276 0.0 68-69 0.0 0.0 0.0 1.0973243085875382 0.0 70-71 0.0 0.0 0.0 1.2687511252331 0.0 72-73 0.0 0.0 0.0 1.4501432169593929 0.0 74-75 0.0 0.0 0.0 1.644330601463059 0.0 76-77 0.0 0.0 0.0 1.8579969375595735 0.0 78-79 0.0 0.0 0.0 2.110831922840471 0.0 80-81 0.0 0.0 0.0 2.389167173962392 0.0 82-83 0.0 0.0 0.0 2.6815621938538037 0.0 84-85 0.0 0.0 0.0 2.992171689345947 0.0 86-87 0.0 0.0 0.0 3.321507472149916 0.0 88-89 0.0 0.0 0.0 3.685526025023378 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 10690 0.0 24.921831 1 GTATCAA 14000 0.0 23.922924 1 GTACTTT 6440 0.0 23.322971 14-15 GAGTACT 6640 0.0 23.156504 12-13 GTACATG 9945 0.0 23.105318 1 TACATGG 9775 0.0 22.913694 2 ACATGGG 10085 0.0 21.503551 3 AGTACTT 6755 0.0 20.619532 12-13 TATCAAC 16605 0.0 20.176031 2 ATCAACG 16550 0.0 20.15706 3 TCAACGC 16560 0.0 20.14489 4 CAACGCA 16645 0.0 20.042017 5 AACGCAG 16950 0.0 19.68138 6 ACTTTTT 7630 0.0 19.591852 16-17 TACTTTT 7280 0.0 18.611046 14-15 AGAGTAC 13750 0.0 18.430117 10-11 GTATTAG 605 0.0 18.085981 1 ACGCAGA 18415 0.0 18.064095 7 CGCAGAG 18525 0.0 17.931215 8 CATGGGA 5620 0.0 17.56293 4 >>END_MODULE