##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939303_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 678794 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.948169842396958 33.0 31.0 34.0 30.0 34.0 2 31.916976284410293 33.0 31.0 34.0 30.0 34.0 3 32.02300550682534 34.0 31.0 34.0 30.0 34.0 4 35.42429662018226 37.0 35.0 37.0 33.0 37.0 5 35.50281823351414 37.0 35.0 37.0 33.0 37.0 6 35.6881837494144 37.0 35.0 37.0 33.0 37.0 7 35.66268264009405 37.0 35.0 37.0 33.0 37.0 8 35.70104037454662 37.0 35.0 37.0 33.0 37.0 9 37.23345226976078 39.0 37.0 39.0 34.0 39.0 10-11 37.14988715280336 39.0 37.0 39.0 33.5 39.0 12-13 37.13825844070513 39.0 37.0 39.0 34.0 39.0 14-15 38.157566213018974 40.0 38.0 41.0 33.0 41.0 16-17 38.19871787316918 40.0 38.0 41.0 33.0 41.0 18-19 38.25938870997681 40.0 38.0 41.0 33.5 41.0 20-21 38.218373320919156 40.0 38.0 41.0 33.5 41.0 22-23 38.284107549565846 40.0 38.0 41.0 34.0 41.0 24-25 38.06769874218099 40.0 38.0 41.0 33.5 41.0 26-27 37.940536451412356 40.0 37.5 41.0 33.0 41.0 28-29 37.94702442861899 40.0 38.0 41.0 33.0 41.0 30-31 37.97751011352487 40.0 38.0 41.0 33.0 41.0 32-33 37.70891316069382 40.0 37.0 41.0 33.0 41.0 34-35 37.33428256584472 40.0 37.0 41.0 31.5 41.0 36-37 36.99121014622993 39.5 36.0 41.0 30.5 41.0 38-39 36.43260768362714 38.5 35.5 40.5 30.0 41.0 40-41 36.75340825051488 39.0 36.0 40.5 30.5 41.0 42-43 36.88171595506148 40.0 36.5 41.0 31.0 41.0 44-45 36.79292465755442 40.0 36.5 41.0 30.5 41.0 46-47 36.512782965082195 40.0 36.0 41.0 30.0 41.0 48-49 36.158688055580924 39.0 36.0 41.0 28.5 41.0 50-51 34.939012424977236 38.0 34.0 40.0 26.5 40.5 52-53 35.28627094523523 38.5 34.5 39.5 28.0 40.5 54-55 35.97704310880768 39.0 36.0 40.5 28.0 41.0 56-57 35.764591319310426 39.0 35.5 41.0 28.0 41.0 58-59 35.571062796665856 39.0 35.0 40.0 28.0 41.0 60-61 34.99593691164035 38.5 34.0 40.0 26.0 41.0 62-63 34.79601175025118 38.0 34.0 40.0 26.0 41.0 64-65 34.46095207087865 37.5 34.0 39.5 26.0 41.0 66-67 33.77223502270203 37.0 33.5 39.0 24.0 41.0 68-69 33.27393583325722 36.0 33.0 39.0 23.5 40.0 70-71 32.472548372554854 35.5 31.5 38.0 21.5 39.5 72-73 31.959856009334203 35.0 31.5 37.0 21.5 39.0 74-75 31.610571101099893 35.0 31.0 37.0 20.0 39.0 76-77 31.222183755307206 35.0 31.0 36.0 20.0 37.5 78-79 30.769192126035293 34.0 31.0 36.0 18.0 37.0 80-81 30.17711632689741 34.0 30.0 35.5 10.0 37.0 82-83 29.666790661084217 34.0 29.5 35.0 7.0 36.0 84-85 29.187342551643063 34.0 29.0 35.0 2.0 36.0 86-87 29.11662816701385 34.0 29.0 35.0 2.0 35.5 88-89 28.582156736800854 34.0 29.0 35.0 2.0 35.0 90-91 28.039765378008646 34.0 28.0 35.0 2.0 35.0 92-93 27.524648862541508 33.5 26.5 35.0 2.0 35.0 94-95 26.890029228307853 33.0 25.0 35.0 2.0 35.0 96-97 26.207400183266202 33.0 25.0 35.0 2.0 35.0 98-99 25.567010757313707 33.0 22.0 35.0 2.0 35.0 100-101 22.743688070312935 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1724896609961739 1101 2 0.3252073664929469 1101 3 0.5092427903466046 1101 4 0.32577513378743106 1101 5 0.32582725563298 1101 6 0.27679880771083987 1101 7 0.2868518506536759 1101 8 0.21130055365512135 1101 9 0.24152070088660338 1101 10-11 0.41264219874651076 1101 12-13 0.40806567975144503 1101 14-15 0.6462839846506014 1101 16-17 0.5980674736424874 1101 18-19 0.4474259183300475 1101 20-21 0.47559337499017573 1101 22-23 0.6677466453943666 1101 24-25 0.561206839119734 1101 26-27 0.6936906426489458 1101 28-29 0.6521519828662719 1101 30-31 0.5641835536480428 1101 32-33 0.7055047857181975 1101 34-35 0.6273117839878282 1101 36-37 0.4676560862652863 1101 38-39 0.8638565062260142 1101 40-41 0.6188324142811794 1101 42-43 0.45655811497497467 1101 44-45 0.6675774484228114 1101 46-47 0.645329677131194 1101 48-49 0.07460153903466704 1101 50-51 0.19648299331489483 1101 52-53 0.16008763869685794 1101 54-55 0.06854730085634486 1101 56-57 0.08480910351700999 1101 58-59 -0.013731276063772668 1101 60-61 -0.01793538631742564 1101 62-63 0.1369063093884222 1101 64-65 -0.06346843925766166 1101 66-67 0.16298680563708245 1101 68-69 0.3183038987653788 1101 70-71 0.3342830132295589 1101 72-73 0.3070672443828002 1101 74-75 0.08973397005698658 1101 76-77 0.1636516118999083 1101 78-79 0.020490467575235982 1101 80-81 -0.30736074200025953 1101 82-83 -0.04256386305636184 1101 84-85 -0.05638851557465685 1101 86-87 0.09459683157759713 1101 88-89 0.21005510594293142 1101 90-91 0.32230071469275856 1101 92-93 0.3357956608251307 1101 94-95 0.4356392409041412 1101 96-97 0.5026784422940551 1101 98-99 0.5453716079049649 1101 100-101 0.09627599728652925 1102 1 0.3206684569675069 1102 2 0.40536406902720756 1102 3 0.3463977250587007 1102 4 0.26553898052216596 1102 5 0.25148613828835664 1102 6 0.3662344066148435 1102 7 0.43163388194209773 1102 8 0.5091482084697603 1102 9 0.5335788104452845 1102 10-11 0.7106996196936066 1102 12-13 0.5924341968929525 1102 14-15 0.7207466216857554 1102 16-17 0.6600728957353468 1102 18-19 0.7496121154291941 1102 20-21 0.741911010101262 1102 22-23 0.7045700049508739 1102 24-25 0.6707727534252044 1102 26-27 0.7302145429680706 1102 28-29 0.7267086529952849 1102 30-31 0.7530830192507452 1102 32-33 0.7375990039849256 1102 34-35 0.7219611562807629 1102 36-37 0.7386941826882705 1102 38-39 1.244606629915701 1102 40-41 1.1308469503508292 1102 42-43 0.902654005584111 1102 44-45 0.9283832975705835 1102 46-47 1.0835759743972844 1102 48-49 1.0425695272761928 1102 50-51 0.7991237319231388 1102 52-53 0.9734030141203718 1102 54-55 1.1094922032499923 1102 56-57 1.0479640440157638 1102 58-59 1.1926163917708337 1102 60-61 1.064437603906029 1102 62-63 1.2644036113619634 1102 64-65 1.1600323115604283 1102 66-67 1.0392247874843505 1102 68-69 1.1854105438671212 1102 70-71 1.1131793829734704 1102 72-73 1.1529426635533966 1102 74-75 1.0618220544726675 1102 76-77 1.0419264952265337 1102 78-79 1.121282370891855 1102 80-81 1.3583704159377241 1102 82-83 1.1752616288813833 1102 84-85 1.4972262612197085 1102 86-87 1.2529312016860708 1102 88-89 1.5413264623052108 1102 90-91 1.625768655194399 1102 92-93 1.3247315823349837 1102 94-95 1.7006953858944875 1102 96-97 1.793185729135498 1102 98-99 1.9496343450491942 1102 100-101 1.6219788584965755 1103 1 0.27641563890986376 1103 2 0.3787301474775191 1103 3 0.4547546284376125 1103 4 0.3394279690567785 1103 5 0.3281676598325305 1103 6 0.33750608530798587 1103 7 0.45697087610592746 1103 8 0.3316236841247786 1103 9 0.3670202210607769 1103 10-11 0.5076849533764758 1103 12-13 0.5171737766332853 1103 14-15 0.7431199988018804 1103 16-17 0.4495291520302729 1103 18-19 0.6581897724056986 1103 20-21 0.6097541094817771 1103 22-23 0.6604289434020174 1103 24-25 0.7216605968005325 1103 26-27 0.5546657633215517 1103 28-29 0.5610523939409333 1103 30-31 0.5115330530536326 1103 32-33 0.6466981913357372 1103 34-35 0.6126205196825723 1103 36-37 0.39521814135215294 1103 38-39 0.4686604937935144 1103 40-41 0.6554595967725447 1103 42-43 0.4760863101137005 1103 44-45 0.6624110849296798 1103 46-47 0.5346059279010618 1103 48-49 0.5074158965693698 1103 50-51 0.5043335168263567 1103 52-53 0.4511413935130193 1103 54-55 0.46146449867349304 1103 56-57 0.5456682066007872 1103 58-59 0.4512471675817551 1103 60-61 0.47361681443597803 1103 62-63 0.3116159073373481 1103 64-65 -0.10046659157013238 1103 66-67 0.27179698080932013 1103 68-69 0.12229433089508746 1103 70-71 0.040267229108216895 1103 72-73 -0.10420078489373452 1103 74-75 -0.02147103731944 1103 76-77 0.16593758809481685 1103 78-79 0.2226303475471596 1103 80-81 0.29719545491453303 1103 82-83 0.41591616329825243 1103 84-85 0.4385196013596122 1103 86-87 0.6494115615144302 1103 88-89 0.5040508376415112 1103 90-91 0.4911034418222542 1103 92-93 0.5320716394549798 1103 94-95 0.3709902299182737 1103 96-97 0.677688826584415 1103 98-99 0.5816822485798703 1103 100-101 0.4229543134559428 1104 1 0.02039889942468065 1104 2 0.328588019773143 1104 3 0.10784195801596752 1104 4 0.22768927347326695 1104 5 0.3943675861366813 1104 6 0.210438653608243 1104 7 0.3834750609772186 1104 8 0.3359348911574642 1104 9 0.2850993914561002 1104 10-11 0.41494794226945686 1104 12-13 0.302772818587286 1104 14-15 0.48044431316731107 1104 16-17 0.31055326110013226 1104 18-19 0.3236553722372477 1104 20-21 0.5153470978483625 1104 22-23 0.4060150885828335 1104 24-25 0.5286090623477193 1104 26-27 0.5664663107764198 1104 28-29 0.49039255291340567 1104 30-31 0.6982809606179856 1104 32-33 0.43986684954493427 1104 34-35 0.5839883672374597 1104 36-37 0.4485436664350644 1104 38-39 0.5630846133913465 1104 40-41 0.6465924072163034 1104 42-43 0.4551672176254087 1104 44-45 0.5211427267678701 1104 46-47 0.7317502138409466 1104 48-49 0.48756144586662487 1104 50-51 0.29763903875086584 1104 52-53 0.36112060075627284 1104 54-55 0.4755627773490758 1104 56-57 0.40855839978919306 1104 58-59 0.13321537308788578 1104 60-61 0.46161097140812757 1104 62-63 0.4769406954617921 1104 64-65 0.298048963517509 1104 66-67 0.4730037502992346 1104 68-69 0.46423911164994536 1104 70-71 0.5393355030462033 1104 72-73 0.3596619302718196 1104 74-75 0.43645653019122577 1104 76-77 0.43756731910497493 1104 78-79 0.4079464500896677 1104 80-81 0.23262341722437085 1104 82-83 0.0970460785480256 1104 84-85 0.26819007564502684 1104 86-87 0.41740782408220767 1104 88-89 0.28004704756939347 1104 90-91 0.4456462643731811 1104 92-93 0.4292382973483271 1104 94-95 0.6932174236857129 1104 96-97 0.8364731083131076 1104 98-99 0.5619699255815043 1104 100-101 0.27315039704808797 1105 1 0.09056671908965441 1105 2 0.18708160463280166 1105 3 0.13043970890738166 1105 4 0.19368344245100388 1105 5 0.1167134043591318 1105 6 0.09545881838033665 1105 7 0.20175846491265048 1105 8 0.06170922856438921 1105 9 0.20782056903815516 1105 10-11 0.2739456464314216 1105 12-13 0.23015043315061234 1105 14-15 0.1027256244362178 1105 16-17 0.20297267820107123 1105 18-19 0.2991351109586944 1105 20-21 0.5149737702038308 1105 22-23 0.38485985539274026 1105 24-25 0.3669556526218969 1105 26-27 0.4182006335549886 1105 28-29 0.4672144934044695 1105 30-31 0.47374850904899546 1105 32-33 0.5685772691956785 1105 34-35 0.5974980026461836 1105 36-37 0.46530658560327964 1105 38-39 0.7553648580098411 1105 40-41 0.46629039277080153 1105 42-43 0.411942479462688 1105 44-45 0.23152809232420424 1105 46-47 0.2003881520564832 1105 48-49 0.16178736242408576 1105 50-51 0.20721882928105373 1105 52-53 0.03610587082199146 1105 54-55 0.353620508532849 1105 56-57 0.21917842843637203 1105 58-59 0.27321323978706147 1105 60-61 0.4290032143199838 1105 62-63 0.12904377452598226 1105 64-65 -0.07304887372179536 1105 66-67 0.15851584408320463 1105 68-69 0.24463737859699108 1105 70-71 0.11128361304326262 1105 72-73 -0.04004245518516569 1105 74-75 -0.07772738065712304 1105 76-77 0.04079622228359625 1105 78-79 0.16589553530995715 1105 80-81 0.2166096929890564 1105 82-83 0.05210581097115252 1105 84-85 0.3272393345769551 1105 86-87 0.13607242855807655 1105 88-89 0.03908056070951105 1105 90-91 0.2503755891326769 1105 92-93 0.5353484264638233 1105 94-95 0.7947396862036165 1105 96-97 0.6133565081851096 1105 98-99 0.4211631823191766 1105 100-101 0.16411713787235627 1106 1 -0.18093090074063056 1106 2 -0.08124083227139067 1106 3 -0.02277991196993412 1106 4 -0.18805925572399218 1106 5 -0.10730013399751215 1106 6 -0.06209015467687351 1106 7 -0.09701309966847305 1106 8 0.07389861946008836 1106 9 0.038157688728432504 1106 10-11 0.2913263640130239 1106 12-13 0.18433673165189646 1106 14-15 0.23219955214236876 1106 16-17 0.08037263193690336 1106 18-19 0.1077828580154403 1106 20-21 0.10820215692358204 1106 22-23 0.07645316900292443 1106 24-25 0.12117482303230531 1106 26-27 0.13438944006119868 1106 28-29 0.23601296425195528 1106 30-31 0.17631130048281562 1106 32-33 0.22163983539605425 1106 34-35 -0.07052249968528912 1106 36-37 -0.13279901844848752 1106 38-39 0.4606231459921162 1106 40-41 0.4333600446938135 1106 42-43 0.47845209145241796 1106 44-45 0.19883150004545058 1106 46-47 0.17209883055704012 1106 48-49 0.03646138265857246 1106 50-51 -0.44553084504355667 1106 52-53 -0.04090327243865488 1106 54-55 0.2799302106159516 1106 56-57 0.14140349144086173 1106 58-59 0.2064613496522938 1106 60-61 0.39217084888175435 1106 62-63 0.24953149625918059 1106 64-65 0.2206677217223998 1106 66-67 -0.1384988995767813 1106 68-69 0.012541003029340914 1106 70-71 -0.16831267121353477 1106 72-73 -0.27377999125295105 1106 74-75 -0.18549854347046946 1106 76-77 0.037472507027374036 1106 78-79 0.1868471162052927 1106 80-81 0.17551858910165308 1106 82-83 -0.09105500571316938 1106 84-85 -0.0709624502459647 1106 86-87 0.239891657407437 1106 88-89 0.28282820184566404 1106 90-91 0.48188635772661215 1106 92-93 0.09208047170530875 1106 94-95 -0.004752625422081991 1106 96-97 -0.21903693204280472 1106 98-99 -0.07510281229956561 1106 100-101 -0.04772892419467922 1107 1 0.34235658622846543 1107 2 0.12212646548336892 1107 3 0.30762777318100376 1107 4 0.2923702877179579 1107 5 0.34991254156940244 1107 6 0.2235001873213136 1107 7 0.26251207099434026 1107 8 0.185851181363887 1107 9 0.4041192853925182 1107 10-11 0.5421833211147273 1107 12-13 0.37812449325080877 1107 14-15 0.6118242620996526 1107 16-17 0.46255083866028457 1107 18-19 0.4614421601194394 1107 20-21 0.5878963650369258 1107 22-23 0.462563626233532 1107 24-25 0.4785421880433489 1107 26-27 0.5657566481409191 1107 28-29 0.6118758062781637 1107 30-31 0.48306495193835985 1107 32-33 0.6517964304347856 1107 34-35 0.8887773624393347 1107 36-37 0.6342285902544731 1107 38-39 1.218496054041573 1107 40-41 1.1350156701211773 1107 42-43 0.5930545507903915 1107 44-45 0.7938063349873303 1107 46-47 0.8759391641481926 1107 48-49 0.6062083869517707 1107 50-51 0.7488196274318142 1107 52-53 0.738601497152537 1107 54-55 0.7953330196865878 1107 56-57 0.47652584182366553 1107 58-59 0.6709993550552085 1107 60-61 0.29580829269473696 1107 62-63 0.5708651882926716 1107 64-65 0.5681697113873483 1107 66-67 0.5453203603799537 1107 68-69 0.6816916683060441 1107 70-71 0.7076062177127156 1107 72-73 0.7591867953540756 1107 74-75 0.695407398621871 1107 76-77 0.8165692551862378 1107 78-79 0.8104338936206332 1107 80-81 0.7607468681255405 1107 82-83 0.41262615468184904 1107 84-85 0.7421020821547479 1107 86-87 0.7412793786525746 1107 88-89 0.8205130164985626 1107 90-91 1.0661158809916813 1107 92-93 0.9708745666039214 1107 94-95 1.06519054302127 1107 96-97 1.0741937233696461 1107 98-99 1.1260652050973867 1107 100-101 0.5657851043444033 1108 1 0.24563706458924273 1108 2 0.20440714273795635 1108 3 0.12781295601519815 1108 4 0.32023869618660683 1108 5 0.29477875374254836 1108 6 0.25668583141987966 1108 7 0.3053715715592844 1108 8 0.28695271453895543 1108 9 0.5314282521180047 1108 10-11 0.47252287940118975 1108 12-13 0.3975272193655641 1108 14-15 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-1.7443587591603205 2316 86-87 -1.8195473923110868 2316 88-89 -2.0313403929547498 2316 90-91 -1.9740556342936486 2316 92-93 -2.2253210112876403 2316 94-95 -2.305513251412716 2316 96-97 -1.966912644845003 2316 98-99 -2.3819156527594068 2316 100-101 -1.5002276033471524 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2744.0 3 437.0 4 398.0 5 425.0 6 476.0 7 516.0 8 710.0 9 872.0 10 1102.0 11 1457.0 12 1989.0 13 2809.0 14 2956.0 15 3138.0 16 3962.0 17 5043.0 18 4803.0 19 3524.0 20 3652.0 21 3880.0 22 4416.0 23 5027.0 24 5827.0 25 6701.0 26 7783.0 27 9206.0 28 11222.0 29 13528.0 30 16277.0 31 19912.0 32 25168.0 33 32849.0 34 43956.0 35 65489.0 36 99798.0 37 141472.0 38 113998.0 39 11271.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.54742183044276 20.508925853799802 15.65356280603666 16.290089509720772 2 14.153328462951894 27.54348291307845 30.405791326293418 27.897397297676235 3 29.32078267465989 23.41146502079478 32.29920274390694 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27.000402105705845 29.441368192331957 24.719761835372758 24-25 26.587535865676397 27.543584914571795 25.330244654095473 20.538634565656327 26-27 22.121761919905083 22.43460386020392 36.69185880498806 18.751775414902934 28-29 17.35979582787032 35.54300917785893 28.11653571425936 18.98065928001139 30-31 19.844556336339657 26.40240506095649 27.628100850255443 26.124937752448403 32-33 21.142590265752467 27.740842094234814 30.541428507133535 20.575139132879187 34-35 26.27071474031316 25.570558550487455 27.382744904981443 20.77598180421794 36-37 21.651265336571978 23.386930850174146 35.699779896416615 19.262023916837258 38-39 20.368046687609407 29.565736574278905 30.845088345706124 19.22112839240557 40-41 19.465065235676224 30.971429755043495 29.721754324448085 19.841750684832196 42-43 18.3390616849123 30.60404819954642 23.773978608399034 27.28291150714225 44-45 21.55917614691662 24.41057683627274 27.29971599948498 26.73053101732566 46-47 26.679207054782307 29.22100362077807 28.595710861668223 15.504078462771401 48-49 22.79392173928489 27.0747112145272 29.102722369254636 21.02864467693328 50-51 13.533640397486257 29.68655183335652 36.74801597286153 20.031791796295693 52-53 20.294847932031036 33.19696123090131 24.286100184649996 22.222090652417656 54-55 17.53430394658174 25.84043189231106 29.63710035458264 26.988163806524557 56-57 23.90219763881975 24.324698158439173 27.25069177803042 24.52241242471066 58-59 28.990783076124305 24.532756246311234 26.455278467662367 20.02118220990209 60-61 22.01787996779743 27.677134473828758 34.04948816688561 16.255497391488202 62-63 17.927279078303908 28.879746723029378 32.249002403703834 20.943971794962877 64-65 16.98369012193741 34.78875751424404 27.667070978570486 20.560481385248067 66-67 21.123250726057854 26.905587314932962 24.327748880198232 27.64341307881095 68-69 18.141382978172192 26.94874768964524 29.236415262072136 25.67345407011043 70-71 25.892758675563353 27.057099399362727 25.329425305222173 21.720716619851746 72-73 21.692498294091266 22.147505968830348 35.6688864449454 20.491109292132986 74-75 17.316553993543067 34.9963659402001 27.097873889983465 20.589206176273365 76-77 19.30775195863412 26.319321736917033 27.4862888212086 26.88663748324025 78-79 20.507078307124914 26.91850421517166 30.03762026833572 22.536797209367705 80-81 25.015517622766776 25.425301234233615 27.025077514132317 22.534103628867292 82-83 20.62716795510779 23.74302998733263 34.62224307142265 21.007558986136925 84-85 19.889454523929324 28.144182673809453 30.371296652558676 21.59506614970255 86-87 19.023324370200566 29.72543808112992 29.391429429758293 21.85980811891122 88-89 18.2168268145676 29.232141073219104 24.610853550695413 27.940178561517886 90-91 20.66424050796865 23.692868144417872 27.860265693242653 27.782625654370825 92-93 24.66031245998397 27.53199247087943 29.225082363634613 18.582612705501987 94-95 21.599226677265808 25.31003594973698 29.784545832028797 23.306191540968417 96-97 14.677486684686873 27.186257471822454 35.41976913215837 22.7164867113323 98-99 19.79390401134468 29.944682847834166 25.766365335966213 24.49504780485494 100-101 17.742294734053534 23.947798585299036 29.81764580085113 28.492260879796298 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1691.0 1 996.0 2 329.5 3 339.5 4 266.0 5 250.0 6 435.5 7 802.0 8 1183.5 9 1345.5 10 1128.0 11 859.0 12 799.0 13 786.5 14 773.0 15 772.0 16 854.5 17 853.0 18 744.0 19 725.5 20 779.0 21 866.5 22 1007.0 23 1110.5 24 1159.0 25 1434.5 26 2194.5 27 3132.0 28 3343.0 29 3727.0 30 5171.0 31 6487.5 32 8301.5 33 11099.5 34 14124.5 35 16500.5 36 19166.5 37 21470.5 38 20664.0 39 18822.5 40 17108.5 41 15254.0 42 13425.0 43 12858.0 44 12929.0 45 13398.5 46 20402.0 47 59880.0 48 129047.0 49 122423.5 50 52189.0 51 15973.5 52 5597.5 53 2953.0 54 1970.5 55 1402.5 56 1035.5 57 811.0 58 701.5 59 661.0 60 598.0 61 528.5 62 451.5 63 345.0 64 229.5 65 148.5 66 112.5 67 103.0 68 105.5 69 103.5 70 92.5 71 68.5 72 50.0 73 45.0 74 36.5 75 25.0 76 21.0 77 15.5 78 12.0 79 9.0 80 7.0 81 5.5 82 3.0 83 1.5 84 2.0 85 2.0 86 1.0 87 0.5 88 1.0 89 1.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2940509197193847 2 0.1810564029735089 3 0.17973052207297058 4 0.20050265618140406 5 0.21346682498666752 6 0.10798563334384217 7 0.12507476495078038 8 0.13656573275544567 9 0.1390701744564625 10-11 0.12610600565119903 12-13 0.11586725869704209 14-15 0.1305992687030233 16-17 0.10791197329381226 18-19 0.13634475260535597 20-21 0.16057890906519504 22-23 0.163746291216481 24-25 0.18650724667572194 26-27 0.16728197361791647 28-29 0.1665453731176174 30-31 0.15564368571319134 32-33 0.16418825151666044 34-35 0.34966425749196367 36-37 0.47186628049157775 38-39 0.43945585847841906 40-41 0.42700731002336495 42-43 0.4510204863331143 44-45 0.45536642928487875 46-47 0.4582391712360451 48-49 0.47230824079175715 50-51 0.463763674988288 52-53 0.4701720993408899 54-55 0.45227270718362267 56-57 0.41522170201858 58-59 0.40586687566478197 60-61 0.3610079051965692 62-63 0.4670047171896039 64-65 0.4656051762390357 66-67 0.47554928299307303 68-69 0.48652463044752897 70-71 0.4804845063450768 72-73 0.4715716402914581 74-75 0.4774644442938506 76-77 0.4519780669835031 78-79 0.43628847632713313 80-81 0.43452063512641537 82-83 0.448147744381948 84-85 0.44991558558266576 86-87 0.451241466483204 88-89 0.45912309183640404 90-91 0.4773907842438207 92-93 0.48372554854639255 94-95 0.4813684269454356 96-97 0.4796742457947477 98-99 0.4765805236934917 100-101 0.4572079305356264 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 678794.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 14.41438787025224 #Duplication Level Percentage of deduplicated Percentage of total 1 72.70144311352766 10.479467997654664 2 10.093618412983933 2.909866616381406 3 4.196476023057111 1.8146889925367635 4 2.343526429827072 1.3512199577485953 5 1.5626916315767958 1.1262621649572624 6 1.1089080577245412 0.9590538513893758 7 0.808429745308859 0.8157113940311789 8 0.6408159928048731 0.7389576219000168 9 0.5437226605617105 0.7053686390863797 >10 4.8546666121581294 14.648479509247284 >50 0.7174686235231593 7.150475696603093 >100 0.3771309431339684 9.58184662799023 >500 0.013286455991169618 1.3165997342345395 >1k 0.0183966313723887 6.050583829556537 >5k 0.008176280609950534 8.746541660651095 >10k+ 0.011242385838681984 31.60487570603158 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 46930 6.913732295806975 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 34713 5.113922633376252 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 23993 3.534651160735068 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 14972 2.2056765380955046 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 14857 2.1887347265886263 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 14831 2.1849044039870713 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 14288 2.1049095896545933 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 13568 1.9988391176115288 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 12905 1.9011658912718734 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 12478 1.8382602085463335 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 10997 1.6200791403577521 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 9034 1.3308897839403413 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 8839 1.302162364428678 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 8158 1.201837376287946 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 7770 1.1446771774647388 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 7596 1.1190434800543316 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 6673 0.9830670276991252 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 6250 0.9207506253738248 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 5051 0.7441138254021101 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 4675 0.6887214677796208 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 4106 0.6048963308455879 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 3756 0.5533342958246537 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 2964 0.43665677657728263 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 2654 0.39098754555874093 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2433 0.3584298034455225 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 2418 0.35622000194462533 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 2406 0.35445216074390756 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2252 0.3317648653346965 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 2101 0.3095195302256649 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 2070 0.3049526071238108 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 1938 0.28550635391591556 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 1528 0.22510511289139268 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1259 0.18547600597530325 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1208 0.17796268087225284 No Hit CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC 1155 0.17015471556908282 No Hit CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT 1077 0.15866374776441747 No Hit CTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGC 1071 0.1577798271640586 No Hit AGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTG 902 0.1328827302539504 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC 889 0.13096756895317282 No Hit TCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAA 854 0.12581136545107943 No Hit GCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAG 813 0.11977124134862713 No Hit GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT 775 0.11417307754635427 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 7.366005002990598E-5 0.0 12-13 0.0 0.0 0.0 3.683002501495299E-4 0.0 14-15 0.0 0.0 0.0 4.4196030017943586E-4 0.0 16-17 0.0 0.0 0.0 5.156203502093418E-4 0.0 18-19 0.0 0.0 0.0 8.102605503289657E-4 0.0 20-21 0.0 0.0 0.0 0.0011785608004784957 0.0 22-23 0.0 0.0 0.0 0.0016205211006579316 0.0 24-25 0.0 0.0 0.0 0.0036830025014952987 0.0 26-27 0.0 0.0 0.0 0.007292344952960692 0.0 28-29 0.0 0.0 0.0 0.018562332607536307 0.0 30-31 0.0 0.0 0.0 0.03786126571537168 0.0 32-33 0.0 0.0 0.0 0.07844795328184986 0.0 34-35 0.0 0.0 0.0 0.13104122900320275 0.0 36-37 0.0 0.0 0.0 0.19755625418020784 0.0 38-39 0.0 0.0 0.0 0.31482305382781817 0.0 40-41 0.0 0.0 0.0 0.43768801727770135 0.0 42-43 0.0 0.0 0.0 0.5836085763869452 0.0 44-45 0.0 0.0 0.0 0.7633390984599157 0.0 46-47 0.0 0.0 0.0 0.9877076108510092 0.0 48-49 0.0 0.0 0.0 1.1683957135743688 0.0 50-51 0.0 0.0 0.0 1.3746438536581054 0.0 52-53 0.0 0.0 0.0 1.5883316587948628 0.0 54-55 0.0 0.0 0.0 1.8470994145499224 0.0 56-57 0.0 0.0 0.0 2.238455260358813 0.0 58-59 0.0 0.0 0.0 2.552541713686332 0.0 60-61 0.0 0.0 0.0 2.9111924972819443 0.0 62-63 0.0 0.0 0.0 3.2682227597768985 0.0 64-65 0.0 0.0 0.0 3.646246136530376 0.0 66-67 0.0 0.0 0.0 4.1541321814865775 0.0 68-69 0.0 0.0 0.0 4.743338921675795 0.0 70-71 0.0 0.0 0.0 5.329451939763757 0.0 72-73 0.0 0.0 0.0 5.825184076465025 0.0 74-75 0.0 0.0 0.0 6.506318559091565 0.0 76-77 0.0 0.0 0.0 7.09869268143207 0.0 78-79 0.0 0.0 0.0 8.143265850906166 0.0 80-81 0.0 0.0 0.0 9.127511439405769 0.0 82-83 0.0 0.0 0.0 9.90248882577041 0.0 84-85 0.0 0.0 0.0 10.826038533045372 0.0 86-87 0.0 0.0 0.0 11.691544120896767 0.0 88-89 0.0 0.0 0.0 12.758215305379835 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATGGCTT 20 0.0020914362 71.097244 6 ACACCGT 25 0.0050632157 56.8778 6 CACCGTG 35 2.8168233E-4 54.169327 7 GCAAGCG 20 5.363098E-4 47.54558 94-95 GGCGTTG 50 2.83694E-5 47.46825 1 GTAAGCG 175 0.0 42.1118 94-95 AGGGTCT 95 1.9515937E-8 39.94078 5 TAGGGTC 95 1.9515937E-8 39.94078 4 CTGTTAC 50 0.0016206605 37.974598 1 TACCTGG 50 0.0016271464 37.94374 2 ACCCTTG 160 1.8189894E-12 35.548622 7 GAACGCA 95 8.7384797E-7 34.976604 1 ATACTCA 55 0.002606166 34.47139 6 AGACAGG 55 0.002606166 34.47139 8 GCTTATG 180 0.0 34.28262 1 CTTATGT 180 0.0 34.254765 2 GGACACC 70 2.0784837E-4 33.85583 6 CCTTGGC 175 3.6379788E-12 32.4968 9 CCGACGC 60 1.4935358E-7 31.697052 94-95 GACGCAG 60 0.003962762 31.6455 1 >>END_MODULE