##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939303_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 678794 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.13088359649614 33.0 31.0 34.0 30.0 34.0 2 32.3184765923093 34.0 31.0 34.0 30.0 34.0 3 32.43057392964581 34.0 31.0 34.0 30.0 34.0 4 35.88538348895246 37.0 35.0 37.0 35.0 37.0 5 35.85632459921567 37.0 35.0 37.0 35.0 37.0 6 35.97355898844126 37.0 35.0 37.0 35.0 37.0 7 35.94287810440281 37.0 35.0 37.0 35.0 37.0 8 35.97037098147597 37.0 35.0 37.0 35.0 37.0 9 37.56169323830205 39.0 37.0 39.0 35.0 39.0 10-11 37.46216672510364 39.0 37.0 39.0 34.5 39.0 12-13 37.47948567606667 39.0 37.0 39.0 34.5 39.0 14-15 38.4625851141878 40.0 38.0 41.0 34.0 41.0 16-17 38.58265320553805 40.0 38.0 41.0 34.5 41.0 18-19 38.84521887347266 40.0 38.0 41.0 34.5 41.0 20-21 38.81781512506004 40.0 38.0 41.0 34.5 41.0 22-23 38.76465687675495 40.0 38.0 41.0 34.0 41.0 24-25 38.58703155891183 40.0 38.0 41.0 34.0 41.0 26-27 38.342535437850074 40.0 37.5 41.0 34.0 41.0 28-29 38.32690919483673 40.0 38.0 41.0 34.0 41.0 30-31 38.35248322760661 40.0 38.0 41.0 34.0 41.0 32-33 38.24536972925512 40.0 38.0 41.0 34.0 41.0 34-35 38.00823813999534 40.0 37.0 41.0 33.0 41.0 36-37 37.81988423586537 40.0 37.5 41.0 33.0 41.0 38-39 37.68245299752208 40.0 37.0 41.0 33.0 41.0 40-41 37.856289242391654 40.0 37.0 41.0 33.0 41.0 42-43 37.61873263464321 40.0 37.0 41.0 33.0 41.0 44-45 37.423732384199035 40.0 37.0 41.0 32.0 41.0 46-47 37.1834960238305 40.0 37.0 41.0 31.5 41.0 48-49 36.83358721497244 39.5 36.5 41.0 31.0 41.0 50-51 36.58298983196669 39.0 36.0 40.5 30.5 41.0 52-53 36.615007645913195 39.0 36.0 41.0 31.0 41.0 54-55 36.52358447481858 39.0 36.0 40.5 30.5 41.0 56-57 36.03986113607368 38.5 35.5 40.0 28.5 41.0 58-59 35.7864558319608 39.0 35.0 40.0 28.0 41.0 60-61 35.09848716989248 38.0 34.0 40.0 28.0 41.0 62-63 34.96361561828773 38.0 34.0 40.0 27.5 41.0 64-65 34.75879427337307 37.5 34.0 39.5 27.5 40.5 66-67 34.10248175440561 37.0 33.5 39.0 26.0 40.0 68-69 33.52772564283126 36.0 33.0 39.0 26.0 39.5 70-71 32.76682395542683 35.0 32.0 37.5 25.5 39.0 72-73 32.137968220108014 35.0 31.5 37.0 24.0 39.0 74-75 31.73064950485714 35.0 31.0 36.5 23.0 38.0 76-77 29.79497608994776 33.0 29.0 34.5 20.0 36.0 78-79 30.82354293055036 34.0 31.0 35.0 21.0 36.0 80-81 30.70660097172338 34.0 31.0 35.0 20.5 36.0 82-83 30.319272120849625 34.0 30.5 35.0 19.5 36.0 84-85 29.856771715719347 34.0 30.0 35.0 18.0 35.5 86-87 29.627557108636786 34.0 29.5 35.0 13.5 35.0 88-89 28.951494120454807 34.0 29.0 35.0 6.5 35.0 90-91 28.42679590567978 33.0 29.0 35.0 2.0 35.0 92-93 28.18630689133964 33.0 29.0 35.0 2.0 35.0 94-95 27.633989104205398 33.0 27.0 34.0 2.0 35.0 96-97 26.79032151138637 32.0 25.0 34.0 2.0 35.0 98-99 25.99692248310975 32.0 25.0 34.0 2.0 35.0 100-101 21.565796839689213 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.13203756739758177 1101 2 0.2613618860717253 1101 3 0.2782565733320794 1101 4 0.06281250625746537 1101 5 0.14813294930780785 1101 6 0.24157011869888834 1101 7 0.17506129284605265 1101 8 0.21337287259041204 1101 9 0.3068111840044949 1101 10-11 0.41146940655485054 1101 12-13 0.21042921251533642 1101 14-15 0.39660982391858113 1101 16-17 0.361710089227806 1101 18-19 0.49593815483917325 1101 20-21 0.5163323270521687 1101 22-23 0.3782565322958149 1101 24-25 0.3395145925821623 1101 26-27 0.3669150706421078 1101 28-29 0.40822500366628844 1101 30-31 0.3628281387801593 1101 32-33 0.3707575019197762 1101 34-35 0.6254196690387062 1101 36-37 0.49172693783268784 1101 38-39 0.5349914785601015 1101 40-41 0.43339236382542623 1101 42-43 0.4207705741669585 1101 44-45 0.3559398307403967 1101 46-47 0.49769876351303566 1101 48-49 0.5428762884428338 1101 50-51 0.48828939305953156 1101 52-53 0.354314919586038 1101 54-55 0.3251226483907814 1101 56-57 0.32655054494444613 1101 58-59 0.4987478007030859 1101 60-61 0.35700955058701567 1101 62-63 0.5163946921864522 1101 64-65 0.353401348959963 1101 66-67 0.501673663980597 1101 68-69 0.34357607683968183 1101 70-71 0.34228262765909534 1101 72-73 0.35382356387756886 1101 74-75 0.2029849804803341 1101 76-77 0.46604414653769055 1101 78-79 0.5722686852895258 1101 80-81 0.6615576171150721 1101 82-83 0.5967850545541395 1101 84-85 0.4651088147526785 1101 86-87 0.5791688786402389 1101 88-89 0.4902742223195098 1101 90-91 0.6647931383320405 1101 92-93 0.537980556223637 1101 94-95 0.46426541256757403 1101 96-97 0.42433446417944864 1101 98-99 0.3722086004001852 1101 100-101 0.4886114088369702 1102 1 0.12159844731586844 1102 2 0.11040839049995554 1102 3 0.18782856362207667 1102 4 0.1259188388800041 1102 5 0.19071113420869068 1102 6 0.05909962339041641 1102 7 0.17132022237331057 1102 8 0.22397112560019394 1102 9 0.15999803788864142 1102 10-11 0.15013986540741087 1102 12-13 0.28193776374021695 1102 14-15 0.34967718344037735 1102 16-17 0.38058325449747343 1102 18-19 0.31919499827841236 1102 20-21 0.26779562665896606 1102 22-23 0.21017744033722607 1102 24-25 0.3109921852192272 1102 26-27 0.26984832450542484 1102 28-29 0.22342393429300955 1102 30-31 0.359091408296365 1102 32-33 0.42673467936456433 1102 34-35 0.35917581373972496 1102 36-37 0.2915478409554524 1102 38-39 0.4791271408279485 1102 40-41 0.4419826486155003 1102 42-43 0.31694357191305755 1102 44-45 0.4431779485739895 1102 46-47 0.42056269328622875 1102 48-49 0.5985583466391873 1102 50-51 0.4701352192921675 1102 52-53 0.6013123705658572 1102 54-55 0.7500958127924591 1102 56-57 0.7089672243899656 1102 58-59 0.6351536533228455 1102 60-61 0.7791081956424648 1102 62-63 0.6841960248523904 1102 64-65 0.7339026466166629 1102 66-67 0.9499077224778603 1102 68-69 0.5809025857815016 1102 70-71 0.8364901890771748 1102 72-73 0.9061146440429511 1102 74-75 1.0341550704338438 1102 76-77 1.2553318964848863 1102 78-79 0.8871753899937858 1102 80-81 1.1715598449760627 1102 82-83 1.4928018126981293 1102 84-85 1.4465177487645917 1102 86-87 1.0835247808805413 1102 88-89 1.1518679241275471 1102 90-91 1.3852255169642511 1102 92-93 1.615495652151857 1102 94-95 1.3814772300681462 1102 96-97 1.12556239171899 1102 98-99 0.4854837585927818 1102 100-101 0.6180660458258274 1103 1 0.2726021863299337 1103 2 0.28767352936776547 1103 3 0.44094187920314454 1103 4 0.3223481494389233 1103 5 0.3140323720844904 1103 6 0.12463584767607472 1103 7 0.26741702282586033 1103 8 0.18705836466254766 1103 9 0.3838697800484354 1103 10-11 0.27262499903233106 1103 12-13 0.18055253491532142 1103 14-15 0.309671112716444 1103 16-17 0.3929302791528926 1103 18-19 0.15271229294307176 1103 20-21 0.28406654651161745 1103 22-23 0.4093592723161521 1103 24-25 0.30755532764669624 1103 26-27 0.3740523008296037 1103 28-29 0.35634795366414096 1103 30-31 0.42431029928548014 1103 32-33 0.506433626645844 1103 34-35 0.27054048488585636 1103 36-37 0.4385035108736446 1103 38-39 0.5453628780759772 1103 40-41 0.5631501592047741 1103 42-43 0.6781419880059971 1103 44-45 0.7076421382013365 1103 46-47 0.7966674960566849 1103 48-49 0.6920047242106264 1103 50-51 0.5844966205256199 1103 52-53 0.5132661778755647 1103 54-55 0.4550738064181985 1103 56-57 0.42383773860787954 1103 58-59 0.6003104573626388 1103 60-61 0.31324084096041815 1103 62-63 0.5223574233264259 1103 64-65 0.4167197384639536 1103 66-67 0.3959472649660327 1103 68-69 0.2619412314581808 1103 70-71 0.4060579259388888 1103 72-73 0.2974017754879199 1103 74-75 0.40744664454495805 1103 76-77 0.5603292060504153 1103 78-79 0.8802037154397766 1103 80-81 0.8809984409831841 1103 82-83 1.1234562091439955 1103 84-85 1.1114041296473331 1103 86-87 0.789090530959875 1103 88-89 0.9507738141094748 1103 90-91 1.0231771995205783 1103 92-93 0.9873481887486655 1103 94-95 0.8775243609612282 1103 96-97 0.5618998135929125 1103 98-99 0.6096880108589531 1103 100-101 0.8781841490005426 1104 1 0.15194892528871407 1104 2 0.08920798217610582 1104 3 -0.017088336094005285 1104 4 0.08965351344376415 1104 5 0.09694019963620804 1104 6 0.15831221762922354 1104 7 0.021378174586665466 1104 8 0.09906815568218263 1104 9 0.28097195407591613 1104 10-11 0.18623372672617222 1104 12-13 0.20807793366249427 1104 14-15 0.3088315064628233 1104 16-17 0.2075812766788161 1104 18-19 0.14099991730481776 1104 20-21 0.1146569028836737 1104 22-23 0.12900579545138413 1104 24-25 0.24575872818361688 1104 26-27 0.23541593719323828 1104 28-29 0.21258314869493233 1104 30-31 0.2596395618006113 1104 32-33 0.22100698033327149 1104 34-35 0.14550626895702123 1104 36-37 0.2536861712937082 1104 38-39 0.26352364030159237 1104 40-41 0.2403860385541421 1104 42-43 0.053601271050688126 1104 44-45 0.40403893769599364 1104 46-47 0.3662258473753113 1104 48-49 0.5701657496356916 1104 50-51 0.23459137232853777 1104 52-53 0.31620874775502017 1104 54-55 0.5675955203730254 1104 56-57 0.39418568828413925 1104 58-59 0.5827853218149244 1104 60-61 0.5437996133981713 1104 62-63 0.46552344954109515 1104 64-65 0.501558134000085 1104 66-67 0.4250308044722644 1104 68-69 0.45081315860457494 1104 70-71 0.2888696856904218 1104 72-73 0.4033632430880658 1104 74-75 0.41642700986580294 1104 76-77 0.2717007383976302 1104 78-79 0.15818971322890008 1104 80-81 0.24244630117163268 1104 82-83 0.4404746857455635 1104 84-85 0.3838668869247961 1104 86-87 0.5231880200370966 1104 88-89 0.5418105567530525 1104 90-91 0.4867017033896097 1104 92-93 0.6501699500794018 1104 94-95 0.6922911662462745 1104 96-97 0.7649042353437174 1104 98-99 0.6269815308884326 1104 100-101 0.7449960140318588 1105 1 0.1364850339448367 1105 2 0.20633381644910997 1105 3 0.06495231852458971 1105 4 0.014678147989016566 1105 5 0.08892000438839176 1105 6 -0.015280057790072021 1105 7 0.03731697932868627 1105 8 0.03223742802342855 1105 9 0.1563795669122925 1105 10-11 0.22959179619051184 1105 12-13 0.18452527668710417 1105 14-15 0.23120221319491208 1105 16-17 0.14032769832064673 1105 18-19 0.06767532034628942 1105 20-21 0.021285627271360852 1105 22-23 0.1304699001253411 1105 24-25 0.07887619179052052 1105 26-27 0.35363762778334973 1105 28-29 0.3921461267828832 1105 30-31 0.15781762130954746 1105 32-33 0.2026783707374804 1105 34-35 0.3970592611653956 1105 36-37 0.22441042692410207 1105 38-39 0.38796110919096094 1105 40-41 0.47748972713285553 1105 42-43 0.450303797488381 1105 44-45 0.3440347726800894 1105 46-47 0.6128049471223633 1105 48-49 0.38590062097733835 1105 50-51 0.16824317699944658 1105 52-53 0.25721282134373524 1105 54-55 0.3517245241215434 1105 56-57 0.548921108188857 1105 58-59 0.4543339458358844 1105 60-61 0.5027145251709442 1105 62-63 0.49744804243619 1105 64-65 0.4024091686509905 1105 66-67 0.29119157408593566 1105 68-69 0.20684481272540012 1105 70-71 0.22603762725830023 1105 72-73 0.5287632369929227 1105 74-75 0.2134637534328867 1105 76-77 0.3708700208974527 1105 78-79 0.38395897134814305 1105 80-81 0.5295847828709626 1105 82-83 0.5598633426803161 1105 84-85 0.5805356360994125 1105 86-87 0.8313407846933814 1105 88-89 0.9837301719462168 1105 90-91 1.1685592496873163 1105 92-93 0.8032101577206845 1105 94-95 0.7791482554080922 1105 96-97 0.9965235630491982 1105 98-99 0.5907522416839512 1105 100-101 0.13172916857426742 1106 1 -0.10486295782501287 1106 2 -0.058613771118004365 1106 3 -0.038258171266853935 1106 4 -0.06109768994029707 1106 5 -0.16905386853124327 1106 6 -0.24513262119024404 1106 7 -0.12492415438693882 1106 8 -0.12630987708876518 1106 9 -0.18433411875921024 1106 10-11 -0.09393231596357055 1106 12-13 -0.15112370002253783 1106 14-15 -0.11302146188452866 1106 16-17 -0.10076097061052991 1106 18-19 -0.009668629396209383 1106 20-21 -0.01025557827546919 1106 22-23 -0.10272829313713316 1106 24-25 -0.1425550754824485 1106 26-27 0.015759448643621 1106 28-29 0.014382147090415742 1106 30-31 0.02718708939335812 1106 32-33 -0.12512274203751161 1106 34-35 0.10574961995619958 1106 36-37 0.06453972309999756 1106 38-39 -0.0854869105808902 1106 40-41 -0.030496929306998766 1106 42-43 0.1020332251519207 1106 44-45 0.08161223472521328 1106 46-47 0.0023516800198493115 1106 48-49 -0.020026062490522634 1106 50-51 0.26544419481115966 1106 52-53 0.21199811907801092 1106 54-55 0.10115291814616256 1106 56-57 0.41935221718966176 1106 58-59 0.07294309883636174 1106 60-61 0.27819573339622394 1106 62-63 0.36712431500518505 1106 64-65 0.2580357437633083 1106 66-67 0.25291527960232685 1106 68-69 0.08232480347256654 1106 70-71 0.17999016996134287 1106 72-73 0.26611668306473035 1106 74-75 0.2705719994121658 1106 76-77 0.10782778658161263 1106 78-79 0.19017784956380623 1106 80-81 0.2608167489763673 1106 82-83 0.34409213116784443 1106 84-85 0.24202680329567983 1106 86-87 0.23125190879092372 1106 88-89 0.4467614489590126 1106 90-91 0.49026444953635107 1106 92-93 0.5695322780805689 1106 94-95 0.7939649640777517 1106 96-97 0.6288987411053739 1106 98-99 0.7351122743123639 1106 100-101 0.553384451820552 1107 1 0.08716268923645032 1107 2 0.12267553177065338 1107 3 0.10729787002849633 1107 4 -0.05497736845527612 1107 5 0.14322376539282544 1107 6 0.15191706999133459 1107 7 0.06886360721197349 1107 8 0.043050403594115494 1107 9 0.06706537233493748 1107 10-11 0.1527065514406729 1107 12-13 0.2237583212528591 1107 14-15 0.33261864976089583 1107 16-17 0.12447358566615918 1107 18-19 0.1661830032499907 1107 20-21 0.2693130847817784 1107 22-23 0.2998521061409818 1107 24-25 0.29594017734511624 1107 26-27 0.11335279475564874 1107 28-29 0.09722731192434964 1107 30-31 0.2943604200364405 1107 32-33 0.16532126150465132 1107 34-35 0.31385063888883735 1107 36-37 0.43556908046624443 1107 38-39 0.37485766576059376 1107 40-41 0.2951691881000542 1107 42-43 0.292482833384085 1107 44-45 0.3475020913572422 1107 46-47 0.16494822080517935 1107 48-49 0.25344693971771903 1107 50-51 0.28865433705819044 1107 52-53 0.4241289101239616 1107 54-55 0.5706925489091859 1107 56-57 0.4661199924640158 1107 58-59 0.6959537141192342 1107 60-61 0.6015067600633373 1107 62-63 0.4649564209114061 1107 64-65 0.20980962814975612 1107 66-67 0.37159683232565044 1107 68-69 0.510742823671869 1107 70-71 0.47164814935850785 1107 72-73 0.37595179308479487 1107 74-75 0.37042403559604153 1107 76-77 0.3634857211412523 1107 78-79 0.47898528362462756 1107 80-81 0.48635395851331253 1107 82-83 0.8701303999911971 1107 84-85 0.8691837682439498 1107 86-87 0.7585411444233046 1107 88-89 1.0455833431200467 1107 90-91 1.1000524801603753 1107 92-93 1.0647380262506374 1107 94-95 1.1110166729477342 1107 96-97 1.1761496406581209 1107 98-99 1.2774007813350394 1107 100-101 0.8036899074258024 1108 1 0.11005663651689446 1108 2 0.08214562787444635 1108 3 0.0664759730893465 1108 4 0.1525217746086227 1108 5 0.13712999753999355 1108 6 -0.0036538610432899077 1108 7 0.18480812007024383 1108 8 -0.05714341207787044 1108 9 0.266392941979511 1108 10-11 0.03530630515088262 1108 12-13 -0.04674043671875694 1108 14-15 0.13369521541489604 1108 16-17 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sequence quality scores pass #Quality Count 3 1.0 4 2.0 5 10.0 6 30.0 7 61.0 8 137.0 9 254.0 10 468.0 11 693.0 12 1078.0 13 1364.0 14 1774.0 15 2588.0 16 3466.0 17 4727.0 18 4783.0 19 3648.0 20 3378.0 21 3730.0 22 4151.0 23 4472.0 24 5313.0 25 6308.0 26 7498.0 27 9073.0 28 11305.0 29 13994.0 30 17277.0 31 21283.0 32 26629.0 33 34131.0 34 46463.0 35 67830.0 36 110005.0 37 160811.0 38 95931.0 39 4128.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.02952522934763 21.190553936032362 15.962063264338196 15.817857570281808 2 14.58778362802264 28.65169698023259 29.9431049773569 26.81741441438787 3 29.76838333868596 23.536743106155917 32.08720171362741 14.607671841530715 4 11.10970338571054 17.33707133533885 44.81079679549318 26.742428483457424 5 9.191448362831729 44.00304068686523 35.22467788460122 11.580833065701817 6 24.64591613950624 40.49151878183956 14.417039632053319 20.445525446600886 7 21.8556734443734 35.195066544489194 23.63559489329605 19.313665117841346 8 23.701005017722608 23.943199262220936 20.748857532624037 31.606938187432416 9 17.770634389814877 25.3531999398934 29.021765071582838 27.85440059870889 10-11 25.800979384025197 24.00544200449621 26.66147608847456 23.532102523004035 12-13 32.482756182288 23.733194459585675 24.65740710731091 19.12664225081542 14-15 23.371568485905637 28.10843540341376 32.576806691986526 15.943189418694073 16-17 18.047347520844088 30.228959051370126 30.928144881439678 20.795548546346108 18-19 16.83728789588594 36.32479073179786 27.520425931873294 19.317495440442904 20-21 22.055955120404718 26.88753878201634 24.682967144671284 26.373538952907655 22-23 19.161483454479562 27.396971688023168 28.87753869362428 24.564006163872985 24-25 26.759664935164423 28.170991493737425 25.28631661446624 19.783026956631907 26-27 22.532552241587464 22.57262333832012 36.40606458370624 18.488759836386176 28-29 17.268125528510858 36.34033300235417 27.81793887394408 18.573602595190884 30-31 20.460699416907044 26.502665020610085 27.11529565670881 25.921339905774065 32-33 21.42969737505046 28.13371950842229 30.174913154801015 20.261669961726238 34-35 26.606358106445764 25.678010922424853 27.54347092681504 20.172160044314342 36-37 22.063335496622102 23.7074595156836 35.33853750383978 18.89066748385452 38-39 20.207901953721485 29.929881707147292 31.065151304212037 18.79706503491919 40-41 19.759036677075116 31.07146619169372 29.035152270522435 20.134344860708733 42-43 18.4708876423944 31.016253125955277 23.68964005937013 26.82321917228019 44-45 22.233713274670038 24.222843917623887 27.033732131781747 26.50971067592433 46-47 26.86614328668681 29.68401265186883 28.26487603731918 15.184968024125176 48-49 23.298300510318327 27.455685983335098 28.803889851383047 20.442123654963524 50-51 13.680301888571414 30.074683979737575 36.35929041748337 19.885723714207636 52-53 20.59949012475812 33.530005811782225 24.0209283088303 21.849575754629353 54-55 17.867571015654235 26.210897562441627 28.916431200040073 27.00510022186407 56-57 24.178299490048843 24.697163557828226 27.266586671439864 23.857950280683067 58-59 29.680488401351823 24.569525594222625 25.968862056671764 19.781123947753784 60-61 21.779573124020317 28.688243232094663 33.640496646984644 15.891686996900376 62-63 18.039137403725462 29.111445694384695 31.91747644330778 20.93194045858206 64-65 17.064160849978048 35.54421518163095 26.992136053058807 20.399487915332195 66-67 21.480896563966994 27.18043758650484 23.976876551930896 27.361789297597273 68-69 18.484992011502847 27.55497478227754 28.368335645049967 25.591697561169642 70-71 25.97805814429709 27.879003055418877 24.994622816347817 21.148315983936218 72-73 22.159226510546645 22.53371420489869 35.044579062278096 20.262480222276565 74-75 17.286024920668126 35.926805481486284 26.380536657660496 20.406632940185094 76-77 19.89093935569441 26.62459225955961 26.719045512584287 26.765422872161693 78-79 20.78262445252087 27.608269384039026 29.36675834901067 22.242347814429436 80-81 25.318598366071566 25.880109341058805 26.69604231625671 22.105249976612917 82-83 20.955252992807832 24.358862924539697 33.95757770398677 20.728306378665692 84-85 19.542011272934058 28.86103884241758 30.22161362651998 21.375336258128385 86-87 19.163412360856306 30.223529515865476 28.39917649349041 22.213881629787814 88-89 18.162905461602442 29.95286942057111 24.025136309028742 27.85908880879771 90-91 21.204690603209446 24.138865726054533 27.155393034113008 27.501050636623013 92-93 24.74020100354452 28.66635533018854 28.217102685056144 18.376340981210795 94-95 21.736123183174865 26.26717384066447 29.024564153483972 22.972138822676687 96-97 14.361794594530888 28.42312984498979 34.68209795608093 22.53297760439839 98-99 19.688550117635273 30.78287489705993 24.83216532875265 24.696409656552145 100-101 17.874708213052166 25.462531572655227 28.600755902217394 28.06200431207521 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1005.0 1 619.5 2 254.5 3 263.5 4 218.0 5 212.0 6 385.5 7 745.0 8 1067.0 9 1238.5 10 1083.0 11 768.0 12 664.0 13 622.0 14 595.0 15 565.5 16 540.5 17 562.5 18 603.5 19 645.0 20 696.0 21 796.5 22 899.0 23 1004.5 24 1127.0 25 1464.5 26 2174.5 27 3047.0 28 3336.5 29 3772.0 30 5206.5 31 6461.0 32 8432.0 33 11309.0 34 14031.5 35 16506.5 36 19282.0 37 21506.5 38 20879.0 39 19056.5 40 17315.5 41 15475.5 42 13604.5 43 12980.0 44 13099.0 45 13566.0 46 18762.0 47 61236.0 48 134646.0 49 124876.5 50 51750.5 51 15317.5 52 4593.0 53 1778.5 54 1138.0 55 875.5 56 736.5 57 638.0 58 563.0 59 514.0 60 501.5 61 476.0 62 354.0 63 226.5 64 141.0 65 90.0 66 64.0 67 57.0 68 53.0 69 55.5 70 50.0 71 38.0 72 22.5 73 17.5 74 16.5 75 5.5 76 2.5 77 3.0 78 1.5 79 2.0 80 2.0 81 1.0 82 2.5 83 2.0 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2921357584186071 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0017678412007177435 16-17 6.629404502691538E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 7.366005002990598E-5 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0010312407004186837 36-37 0.00640842435260182 38-39 0.0018415012507476496 40-41 0.002725421851106521 42-43 2.2098015008971793E-4 44-45 7.366005002990598E-4 46-47 1.4732010005981196E-4 48-49 2.9464020011962393E-4 50-51 7.366005002990598E-5 52-53 7.366005002990598E-5 54-55 0.0 56-57 5.156203502093418E-4 58-59 0.0011785608004784957 60-61 2.9464020011962393E-4 62-63 8.839206003588717E-4 64-65 0.0 66-67 3.683002501495299E-4 68-69 8.102605503289658E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.2562633140540429 78-79 1.4732010005981196E-4 80-81 7.366005002990598E-5 82-83 0.0 84-85 0.0 86-87 0.10577583184294499 88-89 0.36557482829842336 90-91 0.2686382024590671 92-93 0.0 94-95 0.0 96-97 0.0 98-99 1.4732010005981196E-4 100-101 3.683002501495299E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 678794.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 12.888151633632589 #Duplication Level Percentage of deduplicated Percentage of total 1 69.85620227698779 9.003173274955287 2 11.175757852864523 2.880697236569563 3 4.493393077591331 1.7373459400053624 4 2.527319281239998 1.3028989649289768 5 1.7317452334141101 1.1159497579530755 6 1.2459421151296237 0.9634734543911703 7 0.9418865163915687 0.8497423371449955 8 0.7795711215765169 0.8037784659263341 9 0.6058250651547712 0.702716877285303 >10 5.32554524255864 14.508378094090402 >50 0.8241507018426226 7.389576219000168 >100 0.4366512733757029 9.97371809414933 >500 0.012573727767363176 1.1457084181651578 >1k 0.021718257052718213 6.488861127234477 >5k 0.009144529285355037 8.693948384929744 >10k+ 0.012573727767363176 32.44003335327065 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 52358 7.713385798931635 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 31981 4.711444120012846 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 21926 3.230140513911437 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 16750 2.4676116760018503 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 16591 2.44418778009234 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 14067 2.072351847541375 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 14063 2.0717625671411355 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 13925 2.0514323933328815 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 13791 2.0316914999248668 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 12451 1.8342825658447186 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 12298 1.8117425905355675 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 9710 1.4304781715807742 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 8894 1.3102649699319675 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 8031 1.18312772358035 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 7835 1.1542529839686269 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 7617 1.1221372021555878 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 5993 0.882889359658453 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 5782 0.8518048185458328 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 5152 0.7589931555081513 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 4823 0.710524842588473 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 4534 0.6679493336711875 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 3790 0.5583431792266873 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 3121 0.45978603228667314 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 2808 0.41367484096795193 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 2490 0.36682704914893177 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2421 0.35666196224480473 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2391 0.3522423592430104 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 2304 0.33942551053780673 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 2253 0.33191218543475637 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 2167 0.31924265682961256 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 2163 0.31865337642937325 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 1820 0.26812258210885775 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1340 0.19740893408014804 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1276 0.18798044767632005 No Hit CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC 1211 0.1784046411724323 No Hit CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT 1130 0.1664717130675875 No Hit AGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTG 1002 0.14761474025993157 No Hit TCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAA 1002 0.14761474025993157 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC 927 0.13656573275544567 No Hit CTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGC 906 0.13347201065418965 No Hit GCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAG 895 0.1318514895535317 No Hit GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT 872 0.12846312725215603 No Hit CGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCA 803 0.11829804034802902 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 2.2098015008971796E-4 0.0 14-15 0.0 0.0 0.0 2.9464020011962393E-4 0.0 16-17 0.0 0.0 0.0 3.683002501495299E-4 0.0 18-19 0.0 0.0 0.0 6.629404502691539E-4 0.0 20-21 0.0 0.0 0.0 0.0010312407004186837 0.0 22-23 0.0 0.0 0.0 0.0014732010005981196 0.0 24-25 0.0 0.0 0.0 0.003314702251345769 0.0 26-27 0.0 0.0 0.0 0.006924044702811162 0.0 28-29 0.0 0.0 0.0 0.018267692407416682 0.0 30-31 0.0 0.0 0.0 0.03771394561531186 0.0 32-33 0.0 0.0 0.0 0.0788899135820293 0.0 34-35 0.0 0.0 0.0 0.1319251496035616 0.0 36-37 0.0 0.0 0.0 0.1994714154809854 0.0 38-39 0.0 0.0 0.0 0.3174748156288948 0.0 40-41 0.0 0.0 0.0 0.4415183398792565 0.0 42-43 0.0 0.0 0.0 0.5889120999890982 0.0 44-45 0.0 0.0 0.0 0.770115823062667 0.0 46-47 0.0 0.0 0.0 0.9942633553036708 0.0 48-49 0.0 0.0 0.0 1.1768666193278077 0.0 50-51 0.0 0.0 0.0 1.3857665212126211 0.0 52-53 0.0 0.0 0.0 1.6013694876501559 0.0 54-55 0.0 0.0 0.0 1.8618314245559036 0.0 56-57 0.0 0.0 0.0 2.2551760917156014 0.0 58-59 0.0 0.0 0.0 2.571914306844197 0.0 60-61 0.0 0.0 0.0 2.9334378323909758 0.0 62-63 0.0 0.0 0.0 3.292604236336797 0.0 64-65 0.0 0.0 0.0 3.6733530349413814 0.0 66-67 0.0 0.0 0.0 4.182859600998241 0.0 68-69 0.0 0.0 0.0 4.7711824205871 0.0 70-71 0.0 0.0 0.0 5.358105699225391 0.0 72-73 0.0 0.0 0.0 5.854574436426957 0.0 74-75 0.0 0.0 0.0 6.5376977404043055 0.0 76-77 0.0 0.0 0.0 7.130513823044988 0.0 78-79 0.0 0.0 0.0 8.174276731968757 0.0 80-81 0.0 0.0 0.0 9.15896428076854 0.0 82-83 0.0 0.0 0.0 9.93526754803372 0.0 84-85 0.0 0.0 0.0 10.857270394258052 0.0 86-87 0.0 0.0 0.0 11.723291602459657 0.0 88-89 0.0 0.0 0.0 12.786058804291141 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGATGCC 25 5.2944364E-5 75.94007 8 ACAGTCC 25 0.005036093 56.95505 8 GTAAGCG 375 0.0 43.032703 94-95 GTATGGT 80 2.2175482E-7 41.664772 1 GCAAGCG 30 8.33018E-5 39.55212 94-95 TCCACTG 50 0.0016216221 37.970036 5 GGGATGC 50 0.0016216221 37.970036 7 GACAATG 120 3.8180588E-9 35.59691 7 GCTTTGC 135 3.0013325E-10 35.271763 1 CTCCGAC 115 0.0 35.08101 94-95 CAGGAGT 55 0.0025479842 34.63046 1 GATCCTC 55 0.00258888 34.51821 5 CTTTGCG 140 4.5838533E-10 33.901817 2 ACCTGGG 85 1.640053E-5 33.50297 3 GGACAAT 130 8.340066E-9 32.85868 6 GTGCTAT 60 0.0039025801 31.744585 1 GTCTCGA 30 0.00396506 31.644024 34-35 CTCGACA 30 0.00396506 31.644024 36-37 GTAGGTC 30 0.0039664907 31.641695 50-51 CCCGTAA 30 0.0039664907 31.641695 22-23 >>END_MODULE