##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939302_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 689526 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.91074883325647 33.0 31.0 34.0 30.0 34.0 2 31.893103378262747 33.0 31.0 34.0 30.0 34.0 3 31.998630943575733 34.0 31.0 34.0 30.0 34.0 4 35.415615944866474 37.0 35.0 37.0 33.0 37.0 5 35.5111931964857 37.0 35.0 37.0 33.0 37.0 6 35.69315877863924 37.0 35.0 37.0 33.0 37.0 7 35.65588534732555 37.0 35.0 37.0 33.0 37.0 8 35.68434692817965 37.0 35.0 37.0 33.0 37.0 9 37.21221099711976 39.0 37.0 39.0 34.0 39.0 10-11 37.14686320747876 39.0 37.0 39.0 33.5 39.0 12-13 37.132747713646765 39.0 37.0 39.0 33.0 39.0 14-15 38.158534993604306 40.0 38.0 41.0 33.0 41.0 16-17 38.21336541334192 40.0 38.0 41.0 33.0 41.0 18-19 38.29156841076333 40.0 38.0 41.0 33.5 41.0 20-21 38.133357552869654 40.0 38.0 41.0 33.5 41.0 22-23 38.12331079611211 40.0 38.0 41.0 33.0 41.0 24-25 38.04407448015013 40.0 38.0 41.0 33.0 41.0 26-27 37.93212511203348 40.0 37.5 41.0 33.0 41.0 28-29 37.950755301467964 40.0 38.0 41.0 33.0 41.0 30-31 37.973647839240286 40.0 38.0 41.0 33.0 41.0 32-33 37.734759820514384 40.0 37.0 41.0 33.0 41.0 34-35 37.33840058242909 40.0 37.0 41.0 31.5 41.0 36-37 36.9972553609291 39.5 36.0 41.0 30.5 41.0 38-39 36.70152684597825 39.0 35.5 40.5 30.0 41.0 40-41 36.92191737512436 39.0 36.0 41.0 31.0 41.0 42-43 36.754534274269574 39.0 36.0 41.0 30.0 41.0 44-45 36.71945075312606 39.5 36.5 41.0 30.0 41.0 46-47 36.509328147161966 40.0 36.0 41.0 30.0 41.0 48-49 36.199346362573706 39.0 36.0 41.0 28.5 41.0 50-51 34.97306555517848 38.0 34.0 40.0 27.0 40.5 52-53 35.33293160809019 38.5 34.5 39.5 28.0 40.5 54-55 36.037557684554315 39.0 36.0 40.5 28.0 41.0 56-57 35.810453848005736 39.0 35.5 41.0 28.0 41.0 58-59 35.63520302352631 39.0 35.0 41.0 28.0 41.0 60-61 35.08239210704165 38.5 34.0 40.0 26.0 41.0 62-63 34.89234706160464 38.0 34.0 40.0 26.0 41.0 64-65 34.583403671507675 37.5 34.0 39.5 26.0 41.0 66-67 33.91218315190435 37.0 33.5 39.0 25.5 41.0 68-69 33.39714963612684 36.5 33.0 39.0 24.0 40.0 70-71 32.60359725376563 35.5 32.0 38.0 22.0 39.5 72-73 32.100595916615184 35.0 31.5 37.0 21.5 39.0 74-75 31.749496030606533 35.0 31.0 37.0 20.5 39.0 76-77 31.372342014659345 35.0 31.0 36.0 20.0 37.5 78-79 30.873407239175897 34.5 31.0 36.0 18.0 37.0 80-81 30.244124949603062 34.0 30.0 35.5 12.0 37.0 82-83 29.71661909775701 34.0 29.5 35.0 9.0 36.0 84-85 29.27573652045028 34.0 29.0 35.0 4.5 36.0 86-87 29.30613493907409 34.0 29.0 35.0 2.0 36.0 88-89 28.801887818588423 34.0 29.0 35.0 2.0 35.0 90-91 28.27711935445509 34.0 29.0 35.0 2.0 35.0 92-93 27.81271699689352 34.0 27.5 35.0 2.0 35.0 94-95 27.21401223449152 33.0 25.5 35.0 2.0 35.0 96-97 26.58417159033887 33.0 25.0 35.0 2.0 35.0 98-99 25.991239634183483 33.0 24.5 35.0 2.0 35.0 100-101 23.418912412294823 30.0 10.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4036435009504622 1101 2 0.3806083592124416 1101 3 0.39787332675652465 1101 4 0.3303440161336155 1101 5 0.3898136154264833 1101 6 0.43285039476030107 1101 7 0.4108990208268395 1101 8 0.3540603679378336 1101 9 0.5515780564011052 1101 10-11 0.4198969861919366 1101 12-13 0.5030291934336901 1101 14-15 0.6546170786216194 1101 16-17 0.5109521037099327 1101 18-19 0.6379731639052295 1101 20-21 0.5335779068700219 1101 22-23 0.6444421661007524 1101 24-25 0.7526181780983663 1101 26-27 0.6227857678436166 1101 28-29 0.38806674864290613 1101 30-31 0.5199863059376426 1101 32-33 0.7065017258506003 1101 34-35 1.0012710490568466 1101 36-37 0.7413754611447203 1101 38-39 1.1514061469114978 1101 40-41 1.0283436908229788 1101 42-43 0.7248082434873524 1101 44-45 1.06581516605754 1101 46-47 0.9820583217867735 1101 48-49 1.0310831614512708 1101 50-51 1.0001204258200787 1101 52-53 1.2064759456018876 1101 54-55 1.2421368393304348 1101 56-57 1.3602586385205555 1101 58-59 1.3498368984780313 1101 60-61 1.326802902854773 1101 62-63 1.422332770521649 1101 64-65 1.353980085795115 1101 66-67 1.1912975370072303 1101 68-69 1.1727217921909698 1101 70-71 1.052776964266819 1101 72-73 1.043352226309871 1101 74-75 1.0581168836968224 1101 76-77 1.0220539398813635 1101 78-79 1.022719914051688 1101 80-81 1.0651525148990366 1101 82-83 0.9475374094508133 1101 84-85 0.7215507184250143 1101 86-87 0.9419909395863364 1101 88-89 0.850193836540786 1101 90-91 1.1922655500942874 1101 92-93 1.169145031200319 1101 94-95 0.8802176508060953 1101 96-97 1.020522680724742 1101 98-99 0.5396551463207011 1101 100-101 0.009042862367369509 1102 1 0.40771802549712177 1102 2 0.43320676699660865 1102 3 0.5446004386539158 1102 4 0.4103860329403375 1102 5 0.3335415013176828 1102 6 0.41777962961213433 1102 7 0.28034561525407753 1102 8 0.3017439150408805 1102 9 0.37321671186329297 1102 10-11 0.4723723842459009 1102 12-13 0.5870337821112628 1102 14-15 0.6458350131638539 1102 16-17 0.7425434902645591 1102 18-19 0.5485685862847021 1102 20-21 0.6108556600765738 1102 22-23 0.6496265424828849 1102 24-25 0.7043983638568747 1102 26-27 0.7318967806697998 1102 28-29 0.6482129781873525 1102 30-31 0.7130051582153953 1102 32-33 0.6865492711845249 1102 34-35 0.7163127894372039 1102 36-37 0.690822608424682 1102 38-39 0.8285291013564304 1102 40-41 0.7832779013492939 1102 42-43 0.6252795504311806 1102 44-45 0.6890432730898297 1102 46-47 0.7010515769835806 1102 48-49 0.7431491720660404 1102 50-51 0.5549813833609889 1102 52-53 0.6897645900070231 1102 54-55 0.8657920250889433 1102 56-57 0.8573533974767713 1102 58-59 0.7389705782657359 1102 60-61 0.72738616247441 1102 62-63 0.8457350261607388 1102 64-65 0.8418380579313407 1102 66-67 0.8626528554503992 1102 68-69 0.9541708191701801 1102 70-71 0.7726976299014936 1102 72-73 0.7703687566061959 1102 74-75 0.6712885997516658 1102 76-77 0.5578248929994594 1102 78-79 0.6142502103807459 1102 80-81 0.6523677957482228 1102 82-83 0.4445782652668271 1102 84-85 0.3235299311627422 1102 86-87 0.403284338524859 1102 88-89 0.49064607006577177 1102 90-91 0.5508637920226356 1102 92-93 0.6133953629119517 1102 94-95 0.39358839384015454 1102 96-97 0.5862375192917533 1102 98-99 0.7389212343348568 1102 100-101 0.37860555586582123 1103 1 0.30901231514280525 1103 2 0.4095999558510961 1103 3 0.26458667876399034 1103 4 0.4210069479730194 1103 5 0.4029321961921255 1103 6 0.3628690688966216 1103 7 0.38508202736278463 1103 8 0.41605850052420124 1103 9 0.5325304373534863 1103 10-11 0.5798876491237834 1103 12-13 0.48640921210782295 1103 14-15 0.6973808507971526 1103 16-17 0.5994675098724045 1103 18-19 0.6513485140452815 1103 20-21 0.7272987285320198 1103 22-23 0.6670145283789921 1103 24-25 0.41879464868659255 1103 26-27 0.5367213420733137 1103 28-29 0.598897747709195 1103 30-31 0.731844382501599 1103 32-33 0.6049144242632991 1103 34-35 0.3772981265545141 1103 36-37 0.6124772351876686 1103 38-39 0.743819779031007 1103 40-41 0.628016893437362 1103 42-43 0.4287764974556012 1103 44-45 0.6050541950024453 1103 46-47 0.9614467438222505 1103 48-49 0.9464192959050592 1103 50-51 0.5379588945408997 1103 52-53 0.6923069446681822 1103 54-55 0.8021041595918703 1103 56-57 0.7593249317044908 1103 58-59 0.4377687378804609 1103 60-61 0.45028562927867455 1103 62-63 0.5484999040417193 1103 64-65 0.5802872753375965 1103 66-67 0.4551397405553175 1103 68-69 0.5453408398100166 1103 70-71 0.6503259838746587 1103 72-73 0.6021057277104305 1103 74-75 0.42177234588169554 1103 76-77 0.4765824179392588 1103 78-79 0.5816134714746575 1103 80-81 0.5665297324527252 1103 82-83 0.5638539360614594 1103 84-85 0.7224377399002719 1103 86-87 0.7541991562063153 1103 88-89 0.7477195134782271 1103 90-91 0.5651880524285531 1103 92-93 0.47115250085484917 1103 94-95 0.8767862782570788 1103 96-97 0.7389400476715231 1103 98-99 0.8886514114934378 1103 100-101 0.7363304440667129 1104 1 0.19766930050355924 1104 2 0.4286240418313305 1104 3 0.4402602652186971 1104 4 0.28253122054229607 1104 5 0.3524385348885559 1104 6 0.32356008809929904 1104 7 0.4241031279182863 1104 8 0.5186291644613306 1104 9 0.3893260103951022 1104 10-11 0.4660496723887704 1104 12-13 0.48776331546748963 1104 14-15 0.7219892803571284 1104 16-17 0.8076688980949029 1104 18-19 0.6024048745992943 1104 20-21 0.6637984779695856 1104 22-23 0.8018317777365951 1104 24-25 0.8126987845660736 1104 26-27 0.6753156868258898 1104 28-29 0.8270854882757916 1104 30-31 0.7901454625856204 1104 32-33 0.9150147558586852 1104 34-35 0.9853238545183416 1104 36-37 1.0983524444339494 1104 38-39 1.170408984588164 1104 40-41 1.1009374918017727 1104 42-43 1.0132080406003254 1104 44-45 1.1207290076137397 1104 46-47 0.976055333315692 1104 48-49 1.0015123771832464 1104 50-51 1.0272126982918692 1104 52-53 1.2255637764928125 1104 54-55 1.2712223397622537 1104 56-57 1.410792587826215 1104 58-59 1.2458312505418547 1104 60-61 1.374234599840257 1104 62-63 1.3591773288996478 1104 64-65 1.3204900176634595 1104 66-67 1.4234236011359869 1104 68-69 1.474350371844686 1104 70-71 1.1815649884487627 1104 72-73 1.3846082066791823 1104 74-75 1.6056198653136171 1104 76-77 1.553297171268543 1104 78-79 1.3587981626431365 1104 80-81 1.2134752697758664 1104 82-83 1.2108847712340278 1104 84-85 1.4065878001418781 1104 86-87 1.4162530751093882 1104 88-89 1.5280215744875143 1104 90-91 1.4543434970969038 1104 92-93 1.6728312691539031 1104 94-95 1.6481094078351717 1104 96-97 1.6428320286074545 1104 98-99 1.245925452159188 1104 100-101 0.5483193233595962 1105 1 0.18113619081637822 1105 2 0.22183488116919037 1105 3 0.11393718176561407 1105 4 0.40685938717231096 1105 5 0.3327629581729852 1105 6 0.23668327587512294 1105 7 0.32539284343425123 1105 8 0.30911687329859205 1105 9 0.3971195331799109 1105 10-11 0.21178471306952673 1105 12-13 0.34076924243134243 1105 14-15 0.4053889234096957 1105 16-17 0.5090501517065675 1105 18-19 0.50469690669388 1105 20-21 0.5406733469269227 1105 22-23 0.6320886980677685 1105 24-25 0.5599296293246212 1105 26-27 0.3774998164724721 1105 28-29 0.47563161785078023 1105 30-31 0.6052027122493868 1105 32-33 0.23952114051301265 1105 34-35 0.4277903715193716 1105 36-37 0.45868269751888846 1105 38-39 0.6238805285136664 1105 40-41 0.5698797409797152 1105 42-43 0.5842152664846338 1105 44-45 0.6909870529941315 1105 46-47 0.5340669622335454 1105 48-49 0.5499828216288165 1105 50-51 0.6527349512540752 1105 52-53 0.5121304331022216 1105 54-55 0.5847147482369408 1105 56-57 0.5760827738173049 1105 58-59 0.4321266168732265 1105 60-61 0.6562396932449062 1105 62-63 0.7139807776868992 1105 64-65 0.7258222395845308 1105 66-67 0.8877544269732525 1105 68-69 0.5673689300429743 1105 70-71 0.4761680761974887 1105 72-73 0.662488971917206 1105 74-75 0.9149726040413917 1105 76-77 0.6155788733657381 1105 78-79 0.6972398160563777 1105 80-81 0.4897553968041528 1105 82-83 0.6443828560934222 1105 84-85 0.5641049187885478 1105 86-87 0.47177808955604306 1105 88-89 0.7460804043340445 1105 90-91 0.5586982046608142 1105 92-93 0.5445948349462704 1105 94-95 0.7605606343945155 1105 96-97 0.6374202861619054 1105 98-99 0.5739706279992696 1105 100-101 0.6093379020133227 1106 1 0.13314372209154968 1106 2 0.1967516586374778 1106 3 0.17385740459818777 1106 4 0.09420956235210554 1106 5 0.1360763310390425 1106 6 0.182872508869103 1106 7 0.1146141911054741 1106 8 0.08134917819877785 1106 9 0.21261299669301081 1106 10-11 0.33745558858026214 1106 12-13 0.32731048489763737 1106 14-15 0.30015002864373486 1106 16-17 0.26561818066703324 1106 18-19 0.3393000104333126 1106 20-21 0.1669702111601552 1106 22-23 0.09249612452622813 1106 24-25 0.1831135341355079 1106 26-27 0.23421876209395265 1106 28-29 0.10104773050934313 1106 30-31 0.1399111179677135 1106 32-33 0.19564369842910168 1106 34-35 0.2247783467127462 1106 36-37 0.23571424929155427 1106 38-39 0.4279873056907917 1106 40-41 0.5190557651263816 1106 42-43 0.335467243929628 1106 44-45 0.33755333500932494 1106 46-47 0.23307557079163388 1106 48-49 0.5721932897131055 1106 50-51 0.5124600985312711 1106 52-53 0.4874135838150693 1106 54-55 0.43192012106417366 1106 56-57 0.5029609826160879 1106 58-59 0.5734326525691387 1106 60-61 0.4494256361586153 1106 62-63 0.6261142831266895 1106 64-65 0.706921262265702 1106 66-67 0.48915246845349003 1106 68-69 0.5475338222661534 1106 70-71 0.5351299054432914 1106 72-73 0.4889736327671912 1106 74-75 0.2905803954172974 1106 76-77 0.6157293047641623 1106 78-79 0.7342192506284242 1106 80-81 0.7679831208256225 1106 82-83 0.7394215315206942 1106 84-85 0.7426475782215647 1106 86-87 0.7542421558623182 1106 88-89 0.7416135623258349 1106 90-91 0.8357418879978695 1106 92-93 0.8897111523856367 1106 94-95 0.9633445857906189 1106 96-97 0.9772011415827713 1106 98-99 1.4688457699385715 1106 100-101 1.1410862060206206 1107 1 0.03764479377528218 1107 2 0.19655319868669707 1107 3 0.24510452692889828 1107 4 0.13417939643958476 1107 5 0.13659232691107803 1107 6 0.11261768629229607 1107 7 0.21451893032909908 1107 8 0.05205648946336083 1107 9 0.08004174957068244 1107 10-11 0.11394999200965827 1107 12-13 0.025373515778014166 1107 14-15 0.10059854813250269 1107 16-17 0.3498445837788893 1107 18-19 0.24693674933939747 1107 20-21 0.17344000555565486 1107 22-23 0.18555399544787576 1107 24-25 0.11355332138644769 1107 26-27 0.10591440391342388 1107 28-29 0.0337896531893378 1107 30-31 0.17628882694604187 1107 32-33 0.1602185972145307 1107 34-35 0.029196065217156786 1107 36-37 0.22170297676634476 1107 38-39 0.23470715962427846 1107 40-41 0.2342260437641528 1107 42-43 0.19285392329773288 1107 44-45 0.3547399667470472 1107 46-47 0.28001134915155745 1107 48-49 0.5819773652866473 1107 50-51 0.2823656315946934 1107 52-53 0.22957441575918125 1107 54-55 0.26295131896844026 1107 56-57 0.25454866222234074 1107 58-59 0.259727008368138 1107 60-61 0.17037109936414652 1107 62-63 0.5080901503966686 1107 64-65 0.5146135699580086 1107 66-67 0.19942078630695192 1107 68-69 0.3087269634902583 1107 70-71 -0.07874641431538265 1107 72-73 -0.030058676512801696 1107 74-75 0.21079949520296637 1107 76-77 0.04118774725047203 1107 78-79 -0.05771533697179976 1107 80-81 -0.04137625045325777 1107 82-83 0.03010582143098972 1107 84-85 -0.11303234556981323 1107 86-87 0.008786888735226484 1107 88-89 0.005009609003614912 1107 90-91 0.4485465240223192 1107 92-93 0.5142646174963765 1107 94-95 0.32456154220881217 1107 96-97 0.3775147083050072 1107 98-99 0.4768238599600032 1107 100-101 0.7419237070129192 1108 1 0.04426657945015222 1108 2 0.12301458999744241 1108 3 0.1461824064859769 1108 4 0.21113926106085046 1108 5 0.13611408683456006 1108 6 0.04551692478694491 1108 7 0.18019600791435408 1108 8 0.15701179241006002 1108 9 0.3393214464400529 1108 10-11 0.17238725059038273 1108 12-13 0.15865534633387313 1108 14-15 0.16828791280895672 1108 16-17 0.201810886247344 1108 18-19 0.30958619936331644 1108 20-21 0.4142706717808622 1108 22-23 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-1.1194649598978934 2316 80-81 -1.0417382414034826 2316 82-83 -0.7833029266836391 2316 84-85 -0.9865165084657406 2316 86-87 -1.0002779679766896 2316 88-89 -1.277705323123083 2316 90-91 -1.501347895283864 2316 92-93 -1.5327037082954789 2316 94-95 -1.5638999962527258 2316 96-97 -1.4788050503676935 2316 98-99 -1.584126366284341 2316 100-101 -1.2027218435149827 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2224.0 3 466.0 4 387.0 5 393.0 6 425.0 7 536.0 8 668.0 9 799.0 10 1077.0 11 1511.0 12 1936.0 13 3088.0 14 2864.0 15 3041.0 16 3901.0 17 5263.0 18 4741.0 19 3615.0 20 3771.0 21 3987.0 22 4441.0 23 4957.0 24 5754.0 25 6716.0 26 8007.0 27 9327.0 28 11144.0 29 13626.0 30 16410.0 31 20108.0 32 25329.0 33 32946.0 34 43982.0 35 64732.0 36 99076.0 37 143214.0 38 121158.0 39 13906.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.303778985212155 20.751550337991134 15.695935962023992 16.248734714772713 2 14.141213228141597 27.58393899885884 30.277130089114863 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24.87756631809319 26.537541370575134 22-23 18.865788532195644 27.08844794292005 29.419730231633306 24.626033293251 24-25 26.563876651982376 27.673544332276162 25.211591636487675 20.550987379253787 26-27 22.11688425734074 22.423511840179174 36.60901100448293 18.85059289799716 28-29 17.350926477599874 35.6489044319832 28.070823484844638 18.929345605572294 30-31 19.9595792468097 26.375521133813013 27.57660530985011 26.08829430952718 32-33 21.120360614530814 27.840158712190476 30.563634358228626 20.475846315050084 34-35 26.20612210039721 25.747128107658256 27.27957931001901 20.767170481925522 36-37 21.617033105777956 23.47116916308087 35.658808110489886 19.252989620651288 38-39 20.424651156023106 29.571784015015933 30.725115311304148 19.278449517656814 40-41 19.548355888867473 30.93285712726995 29.60403853777582 19.914748446086758 42-43 18.33090325979087 30.65916108622832 23.79587673270064 27.21405892128017 44-45 21.55432806040809 24.45845124587685 27.280129641339695 26.707091052375365 46-47 26.606009975388993 29.31773229455778 28.576491323565584 15.499766406487641 48-49 22.785987460472835 27.157719305267985 29.012448555552727 21.043844678706456 50-51 13.530829553988832 29.782132190700196 36.67628240581718 20.01075584949379 52-53 20.391738772896307 33.11877333294521 24.246177581451537 22.24331031270695 54-55 17.58335631454598 25.947693062196258 29.60232436471803 26.866626258539732 56-57 23.852787871513115 24.345507651233255 27.355586837041646 24.446117640211988 58-59 29.00330374846705 24.56571928175839 26.43071511388549 20.000261855889065 60-61 21.97267620113259 27.795432849775814 33.96411742937305 16.267773519718546 62-63 17.941371415017944 28.818093650619264 32.2590361664929 20.981498767869887 64-65 17.022002822311542 34.7989994548965 27.604652229065735 20.574345493726216 66-67 21.115483625390922 26.854475333463856 24.419299454798335 27.610741586346887 68-69 18.122071605500814 26.964558283284795 29.209518187436174 25.703851923778213 70-71 25.825252470844113 27.160942247248915 25.31608898387127 21.6977162980357 72-73 21.66713123040008 22.100449110621238 35.65054812455742 20.58187153442126 74-75 17.31743512148187 35.01973448152135 27.110075684629663 20.55275471236711 76-77 19.431851423864334 26.228080260919302 27.506552817675217 26.83351549754115 78-79 20.416329571447694 26.94894389765498 30.086475836820693 22.54825069407663 80-81 24.953784168977155 25.49244141083964 27.06279751100195 22.490976909181253 82-83 20.620366786095772 23.79576458398214 34.64101414812896 20.942854481793123 84-85 19.868000247408578 28.178745420212554 30.396836080902606 21.556418251476263 86-87 19.048797116834983 29.64979848259834 29.403422630633408 21.89798176993327 88-89 18.253731451900475 29.22743845196597 24.699555355024632 27.819274741108917 90-91 20.685885154926133 23.734255533492114 27.873892864837796 27.70596644674396 92-93 24.615049796961742 27.626086431192263 29.239620082991298 18.519243688854694 94-95 21.584537771576347 25.398125159675867 29.716342874944406 23.300994193803376 96-97 14.661616099120787 27.23186632494783 35.37168474617477 22.734832829756616 98-99 19.84638540624968 29.990363606456675 25.74518289496477 24.418068092328877 100-101 17.69549091845786 24.003853679514446 29.845059671636815 28.455595730390876 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1523.0 1 876.5 2 265.5 3 299.5 4 250.5 5 245.5 6 454.5 7 828.0 8 1175.0 9 1363.5 10 1144.5 11 837.0 12 770.5 13 729.0 14 715.0 15 803.5 16 963.5 17 918.0 18 738.5 19 697.5 20 765.0 21 844.5 22 938.5 23 1052.0 24 1166.5 25 1495.5 26 2206.5 27 3119.5 28 3421.5 29 3768.5 30 5237.5 31 6590.5 32 8563.5 33 11413.5 34 14288.0 35 16776.0 36 19548.0 37 21900.0 38 21032.5 39 19321.0 40 17520.0 41 15542.0 42 13788.0 43 13014.5 44 13206.5 45 13823.0 46 20769.5 47 60819.5 48 130917.5 49 124082.5 50 52984.5 51 16316.0 52 5757.0 53 3036.0 54 2013.0 55 1431.5 56 1008.0 57 796.0 58 695.0 59 642.0 60 590.5 61 525.5 62 456.5 63 349.5 64 232.5 65 154.0 66 123.5 67 104.5 68 85.5 69 82.0 70 83.5 71 69.0 72 47.5 73 40.5 74 32.0 75 21.5 76 17.0 77 10.0 78 9.0 79 9.0 80 10.0 81 5.5 82 1.0 83 2.0 84 4.0 85 4.5 86 2.0 87 0.5 88 0.5 89 1.0 90 1.0 91 0.5 92 0.0 93 0.5 94 0.5 95 0.5 96 0.5 97 0.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.28048253437868914 2 0.10906042701798048 3 0.12051757294141194 4 0.11935735563271001 5 0.12776893112079893 6 0.05540037649051667 7 0.06424703346936882 8 0.06772768539547458 9 0.06511719645089525 10-11 0.062361680342728196 12-13 0.06489965570551363 14-15 0.06098392228864467 16-17 0.06221665317914045 18-19 0.06620490017780331 20-21 0.06954052494032133 22-23 0.07650182879253284 24-25 0.08426078204447693 26-27 0.08404324129909532 28-29 0.07911231773711216 30-31 0.07592172013818188 32-33 0.0696130385221152 34-35 0.23327619263087976 36-37 0.3539387927358794 38-39 0.3271812810539414 40-41 0.3115908609682594 42-43 0.33414258490615295 44-45 0.34799267902878206 46-47 0.3531411433361469 48-49 0.3623503682239683 50-51 0.3564767680986649 52-53 0.3569118495894281 54-55 0.34378689128473766 56-57 0.3217427624194012 58-59 0.30818272262394747 60-61 0.26257167967560324 62-63 0.33378001699718357 64-65 0.3626404225511438 66-67 0.3672812917859515 68-69 0.3784483833822075 70-71 0.3792460327819401 72-73 0.3627854497147316 74-75 0.366991237458776 76-77 0.3505306543915675 78-79 0.33095198730722264 80-81 0.32797893045367393 82-83 0.3434243233757683 84-85 0.34857278768313305 86-87 0.3429167283032112 88-89 0.3569118495894281 90-91 0.3796811142727033 92-93 0.37511275861968946 94-95 0.37525778578327723 96-97 0.3780133018914443 98-99 0.36938418565797376 100-101 0.3466874345564924 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 689526.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 14.322592621598027 #Duplication Level Percentage of deduplicated Percentage of total 1 72.59361266935336 10.397287411932254 2 10.19968002592195 2.9217172376386094 3 4.1627007432309275 1.7886200085275972 4 2.3522145041414366 1.34759240405728 5 1.46418517993479 1.0485463927393601 6 1.1310476113327528 0.9719720503650334 7 0.8282873286214788 0.8304255387033992 8 0.691589542113044 0.7924284218434113 9 0.5640049413718382 0.7270211710653406 >10 4.815812389882339 14.415700060621353 >50 0.7553818424836469 7.367234883093604 >100 0.3908544117944875 9.774395744322911 >500 0.013163490552664088 1.3021989018543174 >1k 0.01822637153445797 6.060105057677303 >5k 0.008100609570870207 8.707430901807909 >10k+ 0.011138338159946536 31.547323813750317 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 47840 6.938099506037481 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 35182 5.102345669343868 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 24126 3.498925348717815 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 15435 2.2384942699767665 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 15183 2.2019474247526563 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 14882 2.1582942485127465 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 14411 2.089986454462921 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 13547 1.9646829851231136 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 13241 1.920304673065265 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 12635 1.8324182119310948 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 11045 1.6018250218265881 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 9100 1.3197471886484338 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 8688 1.259995997250285 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 8259 1.1977793440711446 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 8057 1.168483857026421 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 7769 1.126716033913152 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 6586 0.9551488993888555 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 6455 0.9361503409588616 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 5126 0.7434092405507552 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 4958 0.7190446770680149 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 4103 0.5950464522004971 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 3853 0.5587896613035621 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 2845 0.41260228040712027 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 2681 0.3888178255787309 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2518 0.36517839791392925 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 2461 0.3569118495894281 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 2455 0.35604168660790164 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 2172 0.31499899931257125 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2151 0.3119534288772287 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 2138 0.3100680757505881 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 1961 0.2843982677955581 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 1518 0.2201512343261893 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1349 0.19564164367986123 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1231 0.17852843837650792 No Hit CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT 1148 0.1664911837987255 No Hit CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC 1139 0.16518593932643585 No Hit CTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGC 1105 0.1602550157644527 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC 955 0.1385009412262917 No Hit AGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTG 875 0.12689876813927248 No Hit TCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAA 849 0.12312806188599125 No Hit GCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAG 817 0.11848719265118357 No Hit GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT 777 0.11268610610767396 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.4502716358773997E-4 0.0 12-13 0.0 0.0 0.0 4.3508149076321997E-4 0.0 14-15 0.0 0.0 0.0 5.075950725570899E-4 0.0 16-17 0.0 0.0 0.0 5.801086543509599E-4 0.0 18-19 0.0 0.0 0.0 7.976493997325698E-4 0.0 20-21 0.0 0.0 0.0 0.0012327308904957898 0.0 22-23 0.0 0.0 0.0 0.00195786670843449 0.0 24-25 0.0 0.0 0.0 0.0037707062532812393 0.0 26-27 0.0 0.0 0.0 0.006526222361448299 0.0 28-29 0.0 0.0 0.0 0.015952987994651398 0.0 30-31 0.0 0.0 0.0 0.035169087170026946 0.0 32-33 0.0 0.0 0.0 0.07787958684661636 0.0 34-35 0.0 0.0 0.0 0.13008936573820276 0.0 36-37 0.0 0.0 0.0 0.19629426591600607 0.0 38-39 0.0 0.0 0.0 0.3085452905329168 0.0 40-41 0.0 0.0 0.0 0.42536467080284135 0.0 42-43 0.0 0.0 0.0 0.5668386688826816 0.0 44-45 0.0 0.0 0.0 0.7387683713159475 0.0 46-47 0.0 0.0 0.0 0.9622552304046548 0.0 48-49 0.0 0.0 0.0 1.131284389566166 0.0 50-51 0.0 0.0 0.0 1.3408486409504499 0.0 52-53 0.0 0.0 0.0 1.5604197666222883 0.0 54-55 0.0 0.0 0.0 1.8192207400446103 0.0 56-57 0.0 0.0 0.0 2.222613795563909 0.0 58-59 0.0 0.0 0.0 2.5202820488277453 0.0 60-61 0.0 0.0 0.0 2.87262554276416 0.0 62-63 0.0 0.0 0.0 3.2258391996821008 0.0 64-65 0.0 0.0 0.0 3.61088631900755 0.0 66-67 0.0 0.0 0.0 4.120801826182044 0.0 68-69 0.0 0.0 0.0 4.716428387036892 0.0 70-71 0.0 0.0 0.0 5.327500340813835 0.0 72-73 0.0 0.0 0.0 5.834877872625542 0.0 74-75 0.0 0.0 0.0 6.518245867450974 0.0 76-77 0.0 0.0 0.0 7.1011825514918945 0.0 78-79 0.0 0.0 0.0 8.12623454373004 0.0 80-81 0.0 0.0 0.0 9.100454515130684 0.0 82-83 0.0 0.0 0.0 9.857496309058686 0.0 84-85 0.0 7.251358179386999E-5 0.0 10.769282086534808 0.0 86-87 0.0 1.4502716358773997E-4 0.0 11.621534213358162 0.0 88-89 0.0 1.4502716358773997E-4 0.0 12.684075727383739 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGGGCGA 20 0.0020952842 71.06444 6 GTACAGA 40 5.380639E-4 47.510773 1 GACAATG 140 0.0 40.608246 7 GTAAGCG 210 0.0 38.489117 94-95 GCTTATG 150 0.0 38.008617 1 GGACAAT 150 0.0 37.901028 6 CTTATGT 135 3.1286618E-10 35.126694 2 GGCATTC 135 3.1650416E-10 35.093548 7 CATTCCG 135 3.1650416E-10 35.091 9 TCTGTAT 55 0.0026000368 34.488026 3 TCGATCT 35 2.0420531E-4 33.960987 88-89 TGTATGG 70 2.0726373E-4 33.872166 2 TAGGCAT 140 4.638423E-10 33.872166 5 CATATAC 85 1.6606324E-5 33.442085 7 TAGGACA 195 0.0 31.614021 4 GACGACC 100 0.0 30.904497 94-95 ACTCCCG 110 2.7874412E-6 30.148548 6 GCTTTGC 210 1.8189894E-12 29.411432 1 GATCCTC 65 0.0058901585 29.182173 5 CCTTTAG 165 2.6375346E-9 28.794409 1 >>END_MODULE