##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939302_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 689526 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.09228368473415 33.0 31.0 34.0 30.0 34.0 2 32.27075556251686 34.0 31.0 34.0 30.0 34.0 3 32.38204215649591 34.0 31.0 34.0 30.0 34.0 4 35.854443487265165 37.0 35.0 37.0 35.0 37.0 5 35.81974138756189 37.0 35.0 37.0 35.0 37.0 6 35.93820827641017 37.0 35.0 37.0 35.0 37.0 7 35.916596328492325 37.0 35.0 37.0 35.0 37.0 8 35.941147977016094 37.0 35.0 37.0 35.0 37.0 9 37.539144281723964 39.0 37.0 39.0 35.0 39.0 10-11 37.42703973454228 39.0 37.0 39.0 34.5 39.0 12-13 37.4413343369213 39.0 37.0 39.0 34.0 39.0 14-15 38.42099355209231 40.0 38.0 41.0 34.0 41.0 16-17 38.47302277216522 40.0 38.0 41.0 34.0 41.0 18-19 38.762490464463994 40.0 38.0 41.0 34.0 41.0 20-21 38.75209274197057 40.0 38.0 41.0 34.0 41.0 22-23 38.72249704869722 40.0 38.0 41.0 34.0 41.0 24-25 38.5389999796962 40.0 38.0 41.0 34.0 41.0 26-27 38.289421283606416 40.0 37.5 41.0 34.0 41.0 28-29 38.28826324170517 40.0 38.0 41.0 34.0 41.0 30-31 38.317322334473246 40.0 38.0 41.0 34.0 41.0 32-33 38.062793136154404 40.0 37.0 41.0 33.0 41.0 34-35 37.881315570406336 40.0 37.0 41.0 33.0 41.0 36-37 37.75743119186224 40.0 37.0 41.0 32.5 41.0 38-39 37.64862818805962 40.0 37.0 41.0 33.0 41.0 40-41 37.83565376795074 40.0 37.0 41.0 33.0 41.0 42-43 37.61420163996716 40.0 37.0 41.0 33.0 41.0 44-45 37.425107972723296 40.0 37.0 41.0 32.0 41.0 46-47 37.19883731722952 40.0 37.0 41.0 31.5 41.0 48-49 36.854922802040825 40.0 36.5 41.0 31.0 41.0 50-51 36.61327636666347 39.0 36.0 41.0 30.5 41.0 52-53 36.67994970457967 39.0 36.0 41.0 31.0 41.0 54-55 36.55479053726763 39.5 36.0 41.0 30.5 41.0 56-57 36.164015570116284 39.0 35.5 40.5 29.0 41.0 58-59 35.929510272274 39.0 35.0 40.0 28.5 41.0 60-61 35.25776692974594 38.0 34.0 40.0 28.0 41.0 62-63 35.107704423038435 38.0 34.0 40.0 28.0 41.0 64-65 34.77853554470752 37.5 34.0 39.5 27.5 40.5 66-67 34.102004130373615 37.0 33.5 39.0 26.0 40.0 68-69 33.626846558360384 36.0 33.0 39.0 26.0 40.0 70-71 32.95359928414592 35.5 32.5 37.5 25.5 39.0 72-73 32.3302196001311 35.0 31.5 37.0 25.0 39.0 74-75 31.944925209491736 35.0 31.0 37.0 24.5 38.0 76-77 29.93818072124909 33.0 29.5 34.5 20.5 36.0 78-79 30.99418223533268 34.0 31.0 35.0 21.5 37.0 80-81 30.926841047328168 34.0 31.0 35.0 21.5 36.5 82-83 30.585497138614063 34.0 30.5 35.0 20.5 36.0 84-85 30.199463109440398 34.0 30.0 35.0 19.0 36.0 86-87 30.036217633562764 34.0 30.5 35.0 18.0 35.0 88-89 29.47690587447029 34.0 29.5 35.0 9.0 35.0 90-91 29.002291429184687 34.0 29.0 35.0 4.5 35.0 92-93 28.770580804784736 34.0 29.0 35.0 2.0 35.0 94-95 28.341051678979472 33.0 29.0 35.0 2.0 35.0 96-97 27.700562415340393 33.0 28.0 35.0 2.0 35.0 98-99 27.149584642203486 33.0 27.0 35.0 2.0 35.0 100-101 23.694804836945963 29.5 13.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.18318368964514065 1101 2 0.1367010420423611 1101 3 0.3173162588920775 1101 4 0.15411002415831376 1101 5 0.12437307271166986 1101 6 0.18181779757721728 1101 7 0.08034469390090493 1101 8 0.2419416119629787 1101 9 0.1875521020134343 1101 10-11 0.14974988333472083 1101 12-13 0.2976482639154696 1101 14-15 0.26882291762848354 1101 16-17 0.30146559568733977 1101 18-19 0.273323170281472 1101 20-21 0.33300814503836307 1101 22-23 0.3835583527866433 1101 24-25 0.4001150565158227 1101 26-27 0.41025054164828845 1101 28-29 0.4479944838361192 1101 30-31 0.47371448224249235 1101 32-33 0.2483665378984128 1101 34-35 0.40454834541135654 1101 36-37 0.5279346401157241 1101 38-39 0.6137711441579441 1101 40-41 0.6521696043278382 1101 42-43 0.9263612169955948 1101 44-45 0.8060256223182307 1101 46-47 0.6683336574843395 1101 48-49 0.7826132110831949 1101 50-51 0.46117389709301904 1101 52-53 0.513195441963795 1101 54-55 0.40364047315213014 1101 56-57 0.5531883273712523 1101 58-59 0.538576173274059 1101 60-61 0.48349324222429146 1101 62-63 0.6616742221801175 1101 64-65 0.6265098811438676 1101 66-67 0.6327202049508784 1101 68-69 0.6745254020941331 1101 70-71 0.5729268489098231 1101 72-73 0.401022886479943 1101 74-75 0.5688061678169518 1101 76-77 0.7003462373178415 1101 78-79 0.9489633317439576 1101 80-81 0.8560040672396667 1101 82-83 0.9288904124040798 1101 84-85 0.8631379638663965 1101 86-87 1.124376993307422 1101 88-89 1.318490618625347 1101 90-91 1.491999967393241 1101 92-93 1.3442124698863935 1101 94-95 1.2640627684234786 1101 96-97 1.2356538435655224 1101 98-99 1.2389964337241715 1101 100-101 1.3945205662429352 1102 1 0.30843800189635573 1102 2 0.3315567474824306 1102 3 0.2953928842790745 1102 4 0.16515602150461461 1102 5 0.31463180778465016 1102 6 0.2643614729420989 1102 7 0.24767662667401424 1102 8 0.2276171979868593 1102 9 0.4969671838356362 1102 10-11 0.36693254587342494 1102 12-13 0.2507262714342673 1102 14-15 0.4339503501804529 1102 16-17 0.5616380857359857 1102 18-19 0.46076097435047103 1102 20-21 0.42259792832008714 1102 22-23 0.3761943545557713 1102 24-25 0.4662099813278573 1102 26-27 0.5095705328026483 1102 28-29 0.5743047447826015 1102 30-31 0.6193801169174193 1102 32-33 0.5851768541301752 1102 34-35 0.6677767288700096 1102 36-37 0.6874547639547401 1102 38-39 0.5785019048832893 1102 40-41 0.6360235406392007 1102 42-43 0.6268670772344223 1102 44-45 0.6401117567720149 1102 46-47 0.4464821537249435 1102 48-49 0.5309477422640825 1102 50-51 0.37942356538138 1102 52-53 0.6138547188324353 1102 54-55 0.6623990423692874 1102 56-57 0.8884520381187215 1102 58-59 0.8219698596960043 1102 60-61 0.6566923244887803 1102 62-63 0.9013054694158527 1102 64-65 0.5893457391712857 1102 66-67 0.47212754683500435 1102 68-69 0.4533566714439772 1102 70-71 0.6619458117581161 1102 72-73 0.5985018780765827 1102 74-75 0.5814886092145599 1102 76-77 1.065704485880424 1102 78-79 0.8628261136988478 1102 80-81 0.7869016236306443 1102 82-83 0.6500591430542357 1102 84-85 0.6272274154365007 1102 86-87 0.517655686363593 1102 88-89 0.7042256363166324 1102 90-91 0.9610609470482601 1102 92-93 0.9164122708594178 1102 94-95 0.7161069413558074 1102 96-97 0.6813692349852474 1102 98-99 0.8893281453561919 1102 100-101 0.5553246905242268 1103 1 0.1831370043043421 1103 2 0.2278308272897931 1103 3 0.1176430562777 1103 4 0.20905867028342584 1103 5 0.08170267121774089 1103 6 0.1403612149441642 1103 7 0.20396747634721635 1103 8 0.16098923101059626 1103 9 0.13050261555218867 1103 10-11 0.23488060228896757 1103 12-13 0.2067752480424545 1103 14-15 0.3724877168815155 1103 16-17 0.2334217114669812 1103 18-19 0.3666938518874403 1103 20-21 0.498857818240964 1103 22-23 0.30335293728710866 1103 24-25 0.20688443093225573 1103 26-27 0.21732337077994401 1103 28-29 0.2758656322954991 1103 30-31 0.2665249397588312 1103 32-33 0.273413223239217 1103 34-35 0.609893816933301 1103 36-37 0.5387189538412187 1103 38-39 0.38802417870508776 1103 40-41 0.3867867845332569 1103 42-43 0.44526878002080394 1103 44-45 0.5365578352033822 1103 46-47 0.5007799693424175 1103 48-49 0.4422770766294093 1103 50-51 0.43383963005285864 1103 52-53 0.23327013850907719 1103 54-55 0.483332349902831 1103 56-57 0.5216757223292419 1103 58-59 0.2955089463833076 1103 60-61 0.3732224672476363 1103 62-63 0.4613007394536979 1103 64-65 0.6379944749814044 1103 66-67 0.7317164701236081 1103 68-69 0.6655384739895567 1103 70-71 0.6247242345307384 1103 72-73 0.5012796558543613 1103 74-75 0.3814112098337503 1103 76-77 0.7522369936203646 1103 78-79 0.7978428835646874 1103 80-81 0.7930021998260344 1103 82-83 0.6176159026001571 1103 84-85 0.5273069648001076 1103 86-87 0.7707355367247892 1103 88-89 0.9326129342182519 1103 90-91 0.9115844678600951 1103 92-93 0.7248380534531549 1103 94-95 0.569408239945421 1103 96-97 0.6158592590650542 1103 98-99 0.4062700098212808 1103 100-101 0.4761031992961513 1104 1 0.24953871452621712 1104 2 0.18946811950956288 1104 3 0.10211618212401419 1104 4 0.15509978384685041 1104 5 0.11937766508695802 1104 6 0.04069067788757508 1104 7 0.28040501166585585 1104 8 0.18198849531664507 1104 9 0.29085724136118074 1104 10-11 0.19703901105759059 1104 12-13 0.16161936622262374 1104 14-15 0.5326144374990704 1104 16-17 0.3765475044704303 1104 18-19 0.43655812168454133 1104 20-21 0.39643775831923733 1104 22-23 0.2233009055989612 1104 24-25 0.3091805424165486 1104 26-27 0.2727123289733342 1104 28-29 0.31483342169511275 1104 30-31 0.5199768371028668 1104 32-33 0.5541830896414623 1104 34-35 0.32107130043350196 1104 36-37 0.5626050172388446 1104 38-39 0.5526610231111491 1104 40-41 0.5503120913389523 1104 42-43 0.7346603931097633 1104 44-45 0.8416309247981104 1104 46-47 0.7148962303111901 1104 48-49 0.39113035366616344 1104 50-51 0.43122338507744473 1104 52-53 0.45680381516625346 1104 54-55 0.35185904279858704 1104 56-57 0.5514473373374003 1104 58-59 0.4459623577641665 1104 60-61 0.6656981307049747 1104 62-63 0.8093746073912982 1104 64-65 0.5678796427516133 1104 66-67 0.8256520595850603 1104 68-69 0.6567727871819358 1104 70-71 0.6081537945004527 1104 72-73 0.6490728405561939 1104 74-75 0.4101032739350856 1104 76-77 0.7150884474155816 1104 78-79 0.7621537302524679 1104 80-81 0.6913338272023779 1104 82-83 0.6791063225854437 1104 84-85 0.8106464289163675 1104 86-87 0.982959251659377 1104 88-89 1.0785588873684055 1104 90-91 1.0918121598069135 1104 92-93 0.8861442833948345 1104 94-95 0.8972252014227777 1104 96-97 0.9095664102235084 1104 98-99 0.84154760101001 1104 100-101 0.48052136134074175 1105 1 0.15854524492556266 1105 2 0.1597668200777136 1105 3 0.08869741323469071 1105 4 0.13226128466251197 1105 5 0.007194916182605482 1105 6 0.10815272026102463 1105 7 0.12443994955802395 1105 8 0.1808736738159311 1105 9 0.2648788320785016 1105 10-11 0.18532060064985956 1105 12-13 0.12888426928940788 1105 14-15 0.420449587105054 1105 16-17 0.2673384652113242 1105 18-19 0.19819343186572524 1105 20-21 0.24200685423990365 1105 22-23 0.22753901715270075 1105 24-25 0.34386785544104015 1105 26-27 0.46307449254526745 1105 28-29 0.3825562709333994 1105 30-31 0.360594437986542 1105 32-33 0.5209212651184529 1105 34-35 0.5420987260651842 1105 36-37 0.3227253463668802 1105 38-39 0.2821966334759267 1105 40-41 0.550997348591693 1105 42-43 0.5092555032367372 1105 44-45 0.4742614432923915 1105 46-47 0.48994136012652234 1105 48-49 0.5689597246102309 1105 50-51 0.6647209585508307 1105 52-53 0.45768979142771826 1105 54-55 0.3619819288566717 1105 56-57 0.6807666314201484 1105 58-59 0.5156131322582809 1105 60-61 0.6247540098542288 1105 62-63 0.4261930704717898 1105 64-65 0.5337778667080002 1105 66-67 0.5399381391464999 1105 68-69 0.6150564734568462 1105 70-71 0.5830319567836924 1105 72-73 0.4840818153806552 1105 74-75 0.5080477578698961 1105 76-77 0.6753192359216698 1105 78-79 0.7534640034860125 1105 80-81 0.6975625087981072 1105 82-83 0.9131242313234971 1105 84-85 0.8648686875038187 1105 86-87 0.9257850173320783 1105 88-89 0.7156640334458224 1105 90-91 1.0416298506945232 1105 92-93 1.1298516785479151 1105 94-95 0.5705786663186849 1105 96-97 0.6975278721738292 1105 98-99 0.8388001796739637 1105 100-101 0.6507374730683573 1106 1 0.07933583471369587 1106 2 0.0690760973276312 1106 3 0.03620170780848753 1106 4 0.09356159426003785 1106 5 0.027867101902295133 1106 6 0.06708980111547191 1106 7 0.06482058952231284 1106 8 -0.05779301873140241 1106 9 0.2653495367768173 1106 10-11 0.11383657064122588 1106 12-13 0.0037738720534647996 1106 14-15 0.002303678353825944 1106 16-17 0.08485789997747162 1106 18-19 -0.012391115432812683 1106 20-21 -0.06714665372325612 1106 22-23 -0.012350537085090707 1106 24-25 0.12337909897491528 1106 26-27 0.02446991360759654 1106 28-29 0.12364685004575904 1106 30-31 0.33300240793908387 1106 32-33 0.1183134640372927 1106 34-35 0.1375425957430707 1106 36-37 -0.019588579698513797 1106 38-39 -0.12999267716006102 1106 40-41 0.039564562311035445 1106 42-43 0.07839571500532827 1106 44-45 0.09047940383084097 1106 46-47 0.04739159644940116 1106 48-49 -0.06391487383498173 1106 50-51 0.10252728055165505 1106 52-53 0.44130247425038505 1106 54-55 0.5813715655891016 1106 56-57 0.3444311402858986 1106 58-59 0.339239596538107 1106 60-61 0.23355958455069725 1106 62-63 0.43120098025176645 1106 64-65 0.4045063428864566 1106 66-67 0.4930253796523374 1106 68-69 0.3695718417226175 1106 70-71 0.07385564147947576 1106 72-73 0.3136721567143539 1106 74-75 0.6523090426510905 1106 76-77 0.3965868388216016 1106 78-79 0.6340141941942044 1106 80-81 0.6490823511848234 1106 82-83 0.5774972235495923 1106 84-85 0.39396503153557205 1106 86-87 0.42811427769486343 1106 88-89 0.7953150326014651 1106 90-91 0.45927108636714564 1106 92-93 0.6953402894352649 1106 94-95 0.9538681642660229 1106 96-97 0.9888382752329257 1106 98-99 0.9017120462849881 1106 100-101 1.0195758515149365 1107 1 0.07139743668241749 1107 2 0.008625196319457018 1107 3 0.09288186975180679 1107 4 0.01913911271680746 1107 5 0.049777080468622614 1107 6 0.010647791113086669 1107 7 0.1959232330088554 1107 8 0.10291984941180488 1107 9 0.21521855320929717 1107 10-11 0.17071519253532585 1107 12-13 0.1860990288368214 1107 14-15 0.22926247806804412 1107 16-17 0.23273040930509126 1107 18-19 0.10676423901666254 1107 20-21 0.024561689533072695 1107 22-23 0.14020562708098083 1107 24-25 0.1270704540594565 1107 26-27 0.2295782727407314 1107 28-29 0.05980228387921471 1107 30-31 0.3210749648970861 1107 32-33 0.24808642585359308 1107 34-35 0.24494912110625222 1107 36-37 0.30304776228766883 1107 38-39 0.09396937729734844 1107 40-41 0.21528100273335582 1107 42-43 0.1854648584521783 1107 44-45 0.1195140946302331 1107 46-47 0.267622603832109 1107 48-49 0.4995923104152311 1107 50-51 0.2184675837984571 1107 52-53 0.4404094044718718 1107 54-55 0.3170427571426515 1107 56-57 0.3546319817560928 1107 58-59 0.5679448438191983 1107 60-61 0.5499444380872518 1107 62-63 0.4384249224602357 1107 64-65 0.4901815036390147 1107 66-67 0.4447003816369417 1107 68-69 0.6375721592585322 1107 70-71 0.4504532440832705 1107 72-73 0.4152439595245525 1107 74-75 0.4372181479936401 1107 76-77 0.19511029678778513 1107 78-79 0.48147787602320946 1107 80-81 0.32473925362191025 1107 82-83 0.3911830217555128 1107 84-85 0.17367448616762715 1107 86-87 0.24170084592539354 1107 88-89 0.37712022431377434 1107 90-91 0.4364085000370679 1107 92-93 0.34914675129126493 1107 94-95 0.4533323223989143 1107 96-97 0.6998210489092003 1107 98-99 0.40564155331047047 1107 100-101 0.28624648738061964 1108 1 -0.05522582050038238 1108 2 0.011347485985787387 1108 3 -0.030172980706204555 1108 4 -0.03847042265456224 1108 5 0.022958848485394867 1108 6 0.04662615994655539 1108 7 0.12043487631533623 1108 8 0.039277131536664456 1108 9 3.416080597560267E-4 1108 10-11 0.17141415880100652 1108 12-13 0.07571754040655065 1108 14-15 0.2898518169931137 1108 16-17 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62-63 -0.8776535089123172 2316 64-65 -0.9065153289401664 2316 66-67 -0.9934142488306392 2316 68-69 -0.8613569508470462 2316 70-71 -0.8270731510901825 2316 72-73 -0.5850275336881232 2316 74-75 -0.576235848989775 2316 76-77 -0.649821829909051 2316 78-79 -0.7037257438862916 2316 80-81 -0.6973572772981491 2316 82-83 -0.9269919405370963 2316 84-85 -1.0520721201672139 2316 86-87 -1.2043625024908984 2316 88-89 -1.5166354317948212 2316 90-91 -2.1370756628588587 2316 92-93 -1.6503253452396578 2316 94-95 -1.5387943744336674 2316 96-97 -1.2904806102159903 2316 98-99 -1.2591548267800299 2316 100-101 -0.5461517026646305 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 3.0 5 9.0 6 26.0 7 61.0 8 118.0 9 261.0 10 441.0 11 744.0 12 1071.0 13 1512.0 14 1825.0 15 2493.0 16 3432.0 17 5004.0 18 4807.0 19 3496.0 20 3188.0 21 3580.0 22 3973.0 23 4262.0 24 5162.0 25 6126.0 26 7265.0 27 8839.0 28 11028.0 29 13503.0 30 16900.0 31 20796.0 32 25807.0 33 33012.0 34 44253.0 35 64088.0 36 102938.0 37 165924.0 38 116661.0 39 6918.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.05343418199587 21.2398801101172 15.980082340673995 15.726603367212938 2 14.62265382306106 28.616469864805676 29.99089229412669 26.76998401800657 3 29.6973573150251 23.610277204920482 32.134683826280664 14.557681653773752 4 11.135185620266675 17.32494496219142 44.91839321504918 26.621476202492726 5 9.154404619985323 44.030972001055794 35.27742246122699 11.53720091773189 6 24.65055704933534 40.46431896694251 14.47762665947332 20.407497324248833 7 21.881263360627447 35.211725156121744 23.602880819577507 19.304130663673305 8 23.674379211226263 23.98691274875784 20.768905015909482 31.569803024106413 9 17.730005830091976 25.323918169873217 29.01282910289097 27.93324689714384 10-11 25.76030490510873 23.982126852359446 26.767953637716346 23.489614604815483 12-13 32.463170351806895 23.74515246705708 24.659113651987017 19.13256352914901 14-15 23.28269848820615 28.054986715242087 32.66636403717412 15.995950759377648 16-17 18.058155976940647 30.18839055871796 30.991044559660637 20.76240890468076 18-19 16.830184793611842 36.294788013794985 27.621728549757368 19.2532986428358 20-21 22.07190156716353 26.846268306053723 24.69268744035758 26.389142686425167 22-23 19.14713875908958 27.347699724158335 28.9627947314532 24.542366785298885 24-25 26.74865070059149 28.134460776955716 25.35341383808697 19.76347468436582 26-27 22.46709483923225 22.54395996052323 36.49831513104416 18.490630069200364 28-29 17.2482981062353 36.28021278385442 27.902718679208615 18.56877043070167 30-31 20.453325907942556 26.501901306114632 27.155538732404576 25.889234053538228 32-33 21.404341533169163 28.10321873286867 30.228011706592646 20.264428027369526 34-35 26.497690004474233 25.727063024335628 27.52154267527286 20.25370429591728 36-37 22.020579017873008 23.7030772790207 35.44466388111835 18.831679821987944 38-39 20.15172424795986 29.92148368068725 31.14147078680937 18.785321284543517 40-41 19.701392731801036 31.065034040445283 29.103837319526164 20.129735908227513 42-43 18.46174262641264 30.99053210498225 23.727824124567796 26.819901144037317 44-45 22.17989009761849 24.30382830979767 27.062700048294396 26.453581544289438 46-47 26.81070302019506 29.58812225807621 28.328487001921616 15.272687719807113 48-49 23.260181632019677 27.45958818086628 28.906306651235774 20.37392353587827 50-51 13.663444163091748 30.04375469525442 36.374407926604654 19.91839321504918 52-53 20.49313586434739 33.53354333266621 24.028970626198287 21.944350176788113 54-55 17.851901161087472 26.26115621455899 28.908046977198833 26.97889564715471 56-57 24.196622027305715 24.59733208029864 27.36205741335352 23.843988479042125 58-59 29.613609418111807 24.583316715553156 26.087908661428745 19.71516520490629 60-61 21.783664427145464 28.662704901687075 33.669104396979 15.88452627418846 62-63 18.033387211745058 29.098185610040673 31.934525039683663 20.933902138530605 64-65 16.98811937475889 35.51758744412828 27.050901633876023 20.443391547236796 66-67 21.508761090952337 27.188169844211824 23.96436102481995 27.338708040015895 68-69 18.560068800886405 27.540005743075678 28.37550723250465 25.524418223533267 70-71 26.044630659322493 27.753775782204006 25.091004545151307 21.110589013322194 72-73 22.09662869855524 22.59465197831554 35.111946467573375 20.196772855555846 74-75 17.264033553484566 35.8604316588497 26.45687037182064 20.418664415845086 76-77 19.869824979648794 26.625843121293176 26.75565472729387 26.748677171764157 78-79 20.74816613151643 27.651531631874647 29.3933803801452 22.206921856463715 80-81 25.26168775275937 25.832524189121475 26.75454975928715 22.151238298832006 82-83 20.99130417127128 24.39922497483779 33.98755086827763 20.621919985613303 84-85 19.551256950426815 28.864031233049953 30.226416407793177 21.358295408730054 86-87 19.147538460868414 30.268707073141343 28.432442869441378 22.151311596548865 88-89 18.176651321333605 29.953017955931777 24.067236137167775 27.80309458556685 90-91 21.223304037242446 24.15897756473 27.19238558769142 27.425332810336133 92-93 24.784489634908617 28.623576195821478 28.28638804047998 18.305546128789924 94-95 21.7646615211029 26.309450260033707 29.11006981607655 22.815818402786842 96-97 14.410479082732195 28.44954359951619 34.70804581698152 22.431931500770094 98-99 19.641898927669153 30.813268825250965 24.926978823133574 24.617853423946308 100-101 17.77508832179881 25.511222234468995 28.74939813552538 27.964291308206825 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1105.0 1 671.5 2 258.0 3 276.5 4 230.5 5 220.5 6 436.5 7 756.5 8 1087.5 9 1344.0 10 1132.0 11 754.0 12 663.0 13 645.0 14 616.0 15 596.0 16 565.5 17 564.5 18 584.5 19 666.0 20 768.5 21 839.0 22 937.0 23 1045.0 24 1176.5 25 1508.0 26 2259.0 27 3201.5 28 3411.5 29 3820.0 30 5286.5 31 6618.0 32 8776.0 33 11641.5 34 14335.5 35 16797.5 36 19665.0 37 21895.5 38 20904.5 39 19371.0 40 17788.5 41 15623.5 42 13792.0 43 13134.0 44 13416.5 45 13890.5 46 18777.5 47 62360.0 48 136862.0 49 126148.0 50 52162.0 51 15539.0 52 4632.5 53 1826.5 54 1181.5 55 911.0 56 753.0 57 652.5 58 567.0 59 507.5 60 493.5 61 448.5 62 339.0 63 231.5 64 147.0 65 84.5 66 56.0 67 49.5 68 46.5 69 50.5 70 55.5 71 43.0 72 23.5 73 15.0 74 10.0 75 4.5 76 4.0 77 3.0 78 0.5 79 4.0 80 4.5 81 4.0 82 4.0 83 0.5 84 0.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2748264749987672 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0020303802902283596 16-17 1.4502716358773997E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 2.1754074538160998E-4 26-27 9.426765633203098E-4 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0032631111807241496 36-37 0.019506153502551027 38-39 0.007468898924768609 40-41 0.018200909030261368 42-43 0.00551103221633412 44-45 7.251358179386999E-4 46-47 1.4502716358773997E-4 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 5.075950725570899E-4 60-61 0.0026830025263731897 62-63 0.00152278521767127 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.2329136247219104 78-79 0.0 80-81 0.00166781238125901 82-83 0.0 84-85 0.0 86-87 0.10282425898370764 88-89 0.35604168660790164 90-91 0.35683933600763423 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 2.9005432717547994E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 689526.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 12.841865281367202 #Duplication Level Percentage of deduplicated Percentage of total 1 69.52952071193025 8.928887380606387 2 11.330577765731581 2.9101150645515905 3 4.577178479468762 1.7633852820633307 4 2.6392465103672587 1.3557139252181933 5 1.7798256312960201 1.142814049071391 6 1.1948321814157292 0.9206324344549734 7 0.9655779915977775 0.8679875740726237 8 0.7351944707955007 0.7553014679649498 9 0.6053214075981389 0.6996110371472577 >10 5.3022089714053395 14.378283052415716 >50 0.8390929213533902 7.427421155982515 >100 0.4438270768396802 10.1009969167225 >500 0.015810633780548405 1.422861501959317 >1k 0.020327957717847945 6.312452322319971 >5k 0.009034647874599087 8.711636689551954 >10k+ 0.012422640827573744 32.30190014589733 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 52740 7.648732607617406 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 32448 4.705841404094987 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 22280 3.2312052047348465 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 17153 2.4876509370205038 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 16565 2.402374964830913 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 14327 2.077804172721551 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 14194 2.0585155599643814 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 14170 2.0550349080382757 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 13978 2.0271896926294297 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 12458 1.8067484039760648 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 12417 1.8008022902689675 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 9830 1.425617018067484 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 8914 1.2927721362211142 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 8080 1.1718194817889391 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 7999 1.160072281538332 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 7896 1.145134483688795 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 6225 0.9027940933336814 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 5846 0.8478287983339279 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 5279 0.7655983965796794 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 4926 0.7144038078332072 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 4483 0.6501567743638383 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 3892 0.5644457206834841 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 3142 0.45567534799267906 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 2861 0.4149227150245241 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2541 0.3685140226764473 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 2456 0.3561867137714894 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2400 0.348065192610576 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 2347 0.34037875294042574 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 2224 0.32254041181913373 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 2215 0.3212351673468441 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 2124 0.30803769546035975 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 1767 0.25626299805953656 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1362 0.19752699680650188 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1356 0.19665683382497542 No Hit CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC 1250 0.181283954484675 No Hit CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT 1108 0.1606900972552159 No Hit AGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTG 1072 0.15546911936605726 No Hit CTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGC 963 0.1396611585349936 No Hit TCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAA 935 0.13560039795453688 No Hit GCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAG 934 0.13545537079094916 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC 908 0.1316846645376679 No Hit GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT 899 0.13037942006537825 No Hit CGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCA 734 0.10644993807340114 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.4502716358773997E-4 0.0 12-13 0.0 0.0 0.0 4.3508149076321997E-4 0.0 14-15 0.0 0.0 0.0 5.075950725570899E-4 0.0 16-17 0.0 0.0 0.0 5.801086543509599E-4 0.0 18-19 0.0 0.0 0.0 7.976493997325698E-4 0.0 20-21 0.0 0.0 0.0 0.0012327308904957898 0.0 22-23 0.0 0.0 0.0 0.0018128395448467496 0.0 24-25 0.0 0.0 0.0 0.0037707062532812393 0.0 26-27 0.0 0.0 0.0 0.006526222361448299 0.0 28-29 0.0 0.0 0.0 0.016098015158239137 0.0 30-31 0.0 0.0 0.0 0.035966736569759516 0.0 32-33 0.0 0.0 0.0 0.07896729057352442 0.0 34-35 0.0 0.0 0.0 0.13146712379228628 0.0 36-37 0.0 0.0 0.0 0.19803459187905895 0.0 38-39 0.0 0.0 0.0 0.3115183473864655 0.0 40-41 0.0 0.0 0.0 0.4291353770561226 0.0 42-43 0.0 0.0 0.0 0.571189483790314 0.0 44-45 0.0 0.0 0.0 0.7437718084597245 0.0 46-47 0.0 0.0 0.0 0.9691440206750724 0.0 48-49 0.0 0.0 0.0 1.1384632341637588 0.0 50-51 0.0 0.0 0.0 1.3492602164385388 0.0 52-53 0.0 0.0 0.0 1.569556477928316 0.0 54-55 0.0 0.0 0.0 1.8292276143321644 0.0 56-57 0.0 0.0 0.0 2.2334183192511956 0.0 58-59 0.0 0.0 0.0 2.5324643305691152 0.0 60-61 0.0 0.0 0.0 2.8843727430147665 0.0 62-63 0.0 0.0 0.0 3.2395442666411416 0.0 64-65 0.0 0.0 0.0 3.625969144020675 0.0 66-67 0.0 0.0 0.0 4.138132572230779 0.0 68-69 0.0 0.0 0.0 4.735354431885092 0.0 70-71 0.0 0.0 0.0 5.346788953571004 0.0 72-73 0.0 0.0 0.0 5.854021458219124 0.0 74-75 0.0 0.0 0.0 6.540435023479898 0.0 76-77 0.0 0.0 0.0 7.123081653193643 0.0 78-79 0.0 0.0 0.0 8.148568726922553 0.0 80-81 0.0 0.0 0.0 9.123948915631898 0.0 82-83 0.0 0.0 0.0 9.882586008359365 0.0 84-85 0.0 0.0 0.0 10.79574954388957 0.0 86-87 0.0 0.0 0.0 11.648654292949068 0.0 88-89 0.0 0.0 0.0 12.707787668630335 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGCCCT 25 0.0050361715 56.95492 9 CCCTGTA 25 0.0050376174 56.950794 2 ACTCCCG 100 6.4210326E-10 42.713097 6 GTAAGCG 315 0.0 40.679134 94-95 CAGGTAT 60 8.191711E-5 39.661327 1 GTCTTAA 60 8.191711E-5 39.661327 1 ATATGAG 175 0.0 37.967194 3 CTTATGT 165 0.0 37.39193 2 TACTCCC 115 2.5211193E-9 37.14182 5 TTACTCG 40 1.0841615E-5 35.594246 94-95 CTCCCGC 120 3.8216967E-9 35.594242 7 GGCATTC 155 3.6379788E-11 33.680576 7 GGATATG 175 3.6379788E-12 32.635605 1 CATTCCG 160 5.4569682E-11 32.630424 9 TATGTAC 175 3.6379788E-12 32.54331 4 AGCGTAA 30 0.0039679636 31.639328 92-93 TAGATAT 75 3.0928073E-4 31.639328 7 GATTGGC 170 1.09139364E-10 30.710989 9 GGTAAGC 110 2.6897542E-6 30.286833 1 TAGGGTC 145 2.417255E-8 29.457304 4 >>END_MODULE