FastQCFastQC Report
Fri 27 May 2016
SRR939301_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939301_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2392059
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT173340.7246476780045977No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT92010.3846477030875911No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT76890.3214385598348536No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67330.2814729904237312No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC65210.27261033277189234No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT47890.2002040919559258No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47110.1969433028198719No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT43520.18193531179623915No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC41900.1751629035905887No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC38430.16065657243404113No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG36600.15300625946099156No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34010.14217876732973558No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC33960.14196974238511675No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA31540.13185293506556486No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA26200.10952907098027265No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG80400.027.8592051
TACATGG78850.027.7793772
ACATGGG82900.025.9647163
CATGGGG33750.023.4598334
GTACCGT1903.371497E-722.4429556
GAGTACT67100.020.1945712-13
AGTACTT72600.019.44781312-13
CATGGGA49350.019.3103944
AGAGTAC112550.018.58364310-11
GTACTTT75500.017.9511414-15
GTATAGC10450.017.710511
CCTAGAC6450.017.6414283
ATGGGAT23500.016.9470315
CCTATAC6450.016.9063683
ATAGCCC9150.016.5810153
TGTACCG3001.4964753E-615.8037795
TAGCCCT21050.015.7662414
GTATAGA8650.015.3611851
AGTACAT59900.015.3552582
GAGTACA66500.015.3426231
Produced by FastQC (version 0.11.5)