##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939301_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2392059 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.92686175382798 33.0 31.0 34.0 30.0 34.0 2 31.983360778308562 33.0 31.0 34.0 30.0 34.0 3 32.05121696413006 34.0 31.0 34.0 30.0 34.0 4 35.530294194248555 37.0 35.0 37.0 33.0 37.0 5 35.574696945184044 37.0 35.0 37.0 33.0 37.0 6 35.66581718929174 37.0 35.0 37.0 33.0 37.0 7 35.60600219308972 37.0 35.0 37.0 33.0 37.0 8 35.598750699711005 37.0 35.0 37.0 33.0 37.0 9 37.236559382523595 39.0 37.0 39.0 34.0 39.0 10-11 37.136743491694816 39.0 37.0 39.0 33.0 39.0 12-13 37.05363057516558 39.0 37.0 39.0 33.0 39.0 14-15 38.288789699585166 40.0 38.0 41.0 33.0 41.0 16-17 38.231406917638736 40.0 38.0 41.0 33.0 41.0 18-19 38.172075814183515 40.0 38.0 41.0 33.0 41.0 20-21 38.149150794357496 40.0 38.0 41.0 33.0 41.0 22-23 38.15182694072345 40.0 38.0 41.0 33.0 41.0 24-25 38.06859801534996 40.0 38.0 41.0 33.0 41.0 26-27 37.98606848744115 40.0 38.0 41.0 33.0 41.0 28-29 37.8638074980592 40.0 38.0 41.0 33.0 41.0 30-31 37.71436595000374 40.0 37.5 41.0 32.5 41.0 32-33 37.512633885702655 40.0 37.0 41.0 32.0 41.0 34-35 37.225832013340806 40.0 37.0 41.0 31.0 41.0 36-37 36.99361052549289 40.0 36.5 41.0 30.5 41.0 38-39 36.43759685693371 39.5 35.5 40.5 30.0 41.0 40-41 36.460508708188215 39.0 36.0 40.5 30.0 41.0 42-43 36.65774610910517 40.0 36.0 41.0 30.0 41.0 44-45 36.57744583223072 40.0 36.0 41.0 30.0 41.0 46-47 36.35117131308216 40.0 36.0 41.0 30.0 41.0 48-49 36.13253456540997 40.0 36.0 41.0 29.0 41.0 50-51 35.01671990532006 38.0 34.5 40.0 26.5 40.5 52-53 35.15806382702099 38.5 35.0 39.5 27.5 40.5 54-55 35.70464921642819 39.0 35.0 40.5 28.0 41.0 56-57 35.63269091606854 39.0 35.0 41.0 27.5 41.0 58-59 35.460326856486404 39.0 35.0 41.0 27.0 41.0 60-61 35.17024705494305 39.0 35.0 40.5 26.0 41.0 62-63 34.833350473378786 38.0 34.5 40.0 26.0 41.0 64-65 34.48262563757834 38.0 34.0 40.0 26.0 41.0 66-67 34.11564472280993 37.0 34.0 40.0 24.5 41.0 68-69 33.71005334734637 37.0 33.5 39.5 23.0 41.0 70-71 33.27987687594663 36.0 33.0 39.0 22.5 41.0 72-73 32.8284442398787 36.0 32.5 39.0 22.0 40.0 74-75 32.36395235234583 35.0 32.0 38.0 20.5 40.0 76-77 31.88612927189505 35.0 32.0 37.0 19.5 39.0 78-79 31.465573382596332 35.0 31.0 37.0 18.0 39.0 80-81 31.01637961271022 35.0 31.0 36.0 14.5 38.5 82-83 30.549713656728365 34.5 31.0 36.0 8.5 37.0 84-85 30.087676976194984 34.0 30.5 35.0 4.5 37.0 86-87 29.660003160457165 34.0 30.0 35.0 2.0 36.5 88-89 29.200803784522037 34.0 29.5 35.0 2.0 36.0 90-91 28.750416691227095 34.0 29.0 35.0 2.0 36.0 92-93 28.276553379327183 34.0 29.0 35.0 2.0 35.0 94-95 27.76197409846496 34.0 27.5 35.0 2.0 35.0 96-97 27.140519945369242 34.0 26.5 35.0 2.0 35.0 98-99 26.362118367481738 33.0 25.0 35.0 2.0 35.0 100-101 23.623689465853474 30.0 11.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.34006434223766036 1101 2 0.32941110484284053 1101 3 0.3867712763791431 1101 4 0.283530684057709 1101 5 0.22831869735507127 1101 6 0.3543481751031692 1101 7 0.3151991995635015 1101 8 0.16183381373252814 1101 9 0.31853153344184193 1101 10-11 0.41519361745054084 1101 12-13 0.5304381305046348 1101 14-15 0.5331782231846276 1101 16-17 0.563104780297671 1101 18-19 0.5976988689585241 1101 20-21 0.5240021997516706 1101 22-23 0.7002100810302281 1101 24-25 0.6390105984774408 1101 26-27 0.6532872606830793 1101 28-29 0.7167884215894063 1101 30-31 0.6772576782157032 1101 32-33 0.6878058262942659 1101 34-35 0.7161343689842852 1101 36-37 0.588931927680683 1101 38-39 1.0284426895930139 1101 40-41 0.7991911582284814 1101 42-43 0.6773820021121963 1101 44-45 0.5785006854717452 1101 46-47 0.6964731042026102 1101 48-49 0.5299132214181412 1101 50-51 0.5662520573201917 1101 52-53 0.6030133764199874 1101 54-55 0.6521264494010595 1101 56-57 0.6149644175611115 1101 58-59 0.5570685693308945 1101 60-61 0.5943122646498225 1101 62-63 0.6410304029358684 1101 64-65 0.5766801860053832 1101 66-67 0.535812287686035 1101 68-69 0.616992243311536 1101 70-71 0.5392942606171758 1101 72-73 0.4899068752761693 1101 74-75 0.392660773126849 1101 76-77 0.43384436793767733 1101 78-79 0.3979723209481101 1101 80-81 0.32122479334683973 1101 82-83 0.1778636605373869 1101 84-85 0.22345785438322352 1101 86-87 0.18901177483529708 1101 88-89 0.18968213550108004 1101 90-91 0.30703043025069476 1101 92-93 0.1325148016516664 1101 94-95 0.235385769978258 1101 96-97 0.45582416374524115 1101 98-99 0.41404052335380115 1101 100-101 0.2286670217393656 1102 1 0.3560756186232581 1102 2 0.34662719751566584 1102 3 0.4445366957941772 1102 4 0.37615884623516394 1102 5 0.3418580530327162 1102 6 0.3528455669426762 1102 7 0.3798175544619298 1102 8 0.339063406303616 1102 9 0.43228430662866657 1102 10-11 0.4333415505267837 1102 12-13 0.5471423933952977 1102 14-15 0.7276946339984462 1102 16-17 0.6554984313749372 1102 18-19 0.6145303137950648 1102 20-21 0.5921872327321225 1102 22-23 0.6773030615794582 1102 24-25 0.6069762581112599 1102 26-27 0.5544513787476362 1102 28-29 0.5675016331258647 1102 30-31 0.6615268225107584 1102 32-33 0.43575788676587024 1102 34-35 0.4995137359780273 1102 36-37 0.502517689258589 1102 38-39 0.7815961814508867 1102 40-41 0.6786996031096919 1102 42-43 0.5982022450111018 1102 44-45 0.7652534139897895 1102 46-47 0.7325543120464459 1102 48-49 0.694881854007491 1102 50-51 0.7639163763942562 1102 52-53 0.7499947594647125 1102 54-55 0.6780368239736063 1102 56-57 0.8448467153232073 1102 58-59 0.8307144848671939 1102 60-61 0.6981274748660695 1102 62-63 0.7778831398913439 1102 64-65 0.6031600875355352 1102 66-67 0.7135425428440456 1102 68-69 0.756977378533854 1102 70-71 0.7490265953550903 1102 72-73 0.8351884871243129 1102 74-75 0.6820121079789274 1102 76-77 0.6151853495589599 1102 78-79 0.5950826230579658 1102 80-81 0.6101412279840055 1102 82-83 0.5997063222011505 1102 84-85 0.49661303372838006 1102 86-87 0.34327540998069495 1102 88-89 0.3708512663954977 1102 90-91 0.5345235686296128 1102 92-93 0.48051004194489266 1102 94-95 0.4283120962688116 1102 96-97 0.3944052102851501 1102 98-99 0.3498521054723902 1102 100-101 0.10416023952945253 1103 1 0.26433111799912723 1103 2 0.33265472717999955 1103 3 0.31751799135922454 1103 4 0.2749467735933706 1103 5 0.22925866599030797 1103 6 0.2142100532871325 1103 7 0.24626735459501248 1103 8 0.27728680068985767 1103 9 0.3666014320421169 1103 10-11 0.42463835411321327 1103 12-13 0.3384872816106821 1103 14-15 0.44565642857507726 1103 16-17 0.4212182194140084 1103 18-19 0.42434892788919854 1103 20-21 0.4451909435055086 1103 22-23 0.45554428127763913 1103 24-25 0.49782657891829984 1103 26-27 0.5093064691613094 1103 28-29 0.4566119217793485 1103 30-31 0.46909602309165876 1103 32-33 0.5752494525276717 1103 34-35 0.4779485084503108 1103 36-37 0.35017380644908513 1103 38-39 0.7945086042847791 1103 40-41 0.7423376667076838 1103 42-43 0.6476000943167861 1103 44-45 0.6568590034636301 1103 46-47 0.5557006253153816 1103 48-49 0.4363692961480794 1103 50-51 0.35192703272515047 1103 52-53 0.49348754919888904 1103 54-55 0.541135878309241 1103 56-57 0.43898694758653534 1103 58-59 0.5499591433448998 1103 60-61 0.485492631481101 1103 62-63 0.27819520427617306 1103 64-65 0.2948040475718656 1103 66-67 0.2679407078568872 1103 68-69 0.3373479215987416 1103 70-71 0.39764756219612707 1103 72-73 0.3181069083942347 1103 74-75 0.4188252627677187 1103 76-77 0.46615396341024606 1103 78-79 0.5046100580101474 1103 80-81 0.45716593085499824 1103 82-83 0.3151989225110299 1103 84-85 0.40612346906426566 1103 86-87 0.35367674663623916 1103 88-89 0.24403837874353584 1103 90-91 0.2662635522128447 1103 92-93 0.28125996787513685 1103 94-95 0.1993296933001929 1103 96-97 0.4072696699159195 1103 98-99 0.49436887923663875 1103 100-101 0.3831627991859641 1104 1 0.2806413952376303 1104 2 0.37205088597713143 1104 3 0.30882961192070724 1104 4 0.15028114420567107 1104 5 0.18887428871980205 1104 6 0.2098372449477779 1104 7 0.24289210958458085 1104 8 0.2996770745642081 1104 9 0.3427761387888708 1104 10-11 0.38599182794645515 1104 12-13 0.3416583999329035 1104 14-15 0.29717543484314035 1104 16-17 0.32860836479659383 1104 18-19 0.37706717114981103 1104 20-21 0.44426142240599376 1104 22-23 0.5154833528195155 1104 24-25 0.5506034027420696 1104 26-27 0.47193405564210167 1104 28-29 0.5436528265482607 1104 30-31 0.5060432456837773 1104 32-33 0.6269066458470292 1104 34-35 0.46843719767078085 1104 36-37 0.39161678063626226 1104 38-39 0.7336116850500289 1104 40-41 0.5762784156511316 1104 42-43 0.5456719585921093 1104 44-45 0.5082985784595948 1104 46-47 0.503588746368635 1104 48-49 0.546642971923653 1104 50-51 0.33229874771640056 1104 52-53 0.4332325047290553 1104 54-55 0.4900893128940851 1104 56-57 0.4918157521692521 1104 58-59 0.27192603685793415 1104 60-61 0.4307329461223901 1104 62-63 0.4569634588674205 1104 64-65 0.4343846504552218 1104 66-67 0.34025149205380245 1104 68-69 0.2572642617795182 1104 70-71 0.45863646985260687 1104 72-73 0.33331303254530553 1104 74-75 0.3558981736668585 1104 76-77 0.4780117090105698 1104 78-79 0.30768036870323456 1104 80-81 0.28879003170467854 1104 82-83 0.30309821066665776 1104 84-85 0.3140255373144214 1104 86-87 0.2439144096747654 1104 88-89 0.22386020739159207 1104 90-91 0.3047666932524109 1104 92-93 0.3801886969981467 1104 94-95 0.5000290389266837 1104 96-97 0.45733560955282115 1104 98-99 0.47255302195127413 1104 100-101 0.4846561071012907 1105 1 0.20963996023316156 1105 2 0.25279428294329875 1105 3 0.3458032364591901 1105 4 0.35905567383101555 1105 5 0.2886512401396786 1105 6 0.3011601277585214 1105 7 0.24403996192675947 1105 8 0.3674338228754692 1105 9 0.4862521993944142 1105 10-11 0.4730402273521861 1105 12-13 0.35940975114528584 1105 14-15 0.45499350591811094 1105 16-17 0.49396762915784365 1105 18-19 0.4362411144479452 1105 20-21 0.5655423628585012 1105 22-23 0.5131522030900086 1105 24-25 0.4207027944294808 1105 26-27 0.3876944161342806 1105 28-29 0.3678129821964333 1105 30-31 0.5004565394472067 1105 32-33 0.505229760610149 1105 34-35 0.38728373461425747 1105 36-37 0.2905634295104562 1105 38-39 0.7904186554930135 1105 40-41 0.532670423861525 1105 42-43 0.3339743942432847 1105 44-45 0.16532409832498018 1105 46-47 0.27997550304114327 1105 48-49 0.23707511602372477 1105 50-51 0.09903263709270504 1105 52-53 0.06454486555999495 1105 54-55 0.26944825475268175 1105 56-57 0.24673235801222404 1105 58-59 0.3488149411220931 1105 60-61 0.4459367910496539 1105 62-63 0.36046777342735936 1105 64-65 0.29660701886857055 1105 66-67 0.30309430944149796 1105 68-69 0.32158879547868224 1105 70-71 0.21775373888654315 1105 72-73 0.2073569378974014 1105 74-75 0.255305986208505 1105 76-77 0.27788567588202184 1105 78-79 0.3003047746679073 1105 80-81 0.27224229970551406 1105 82-83 0.1740930859747678 1105 84-85 0.4317793649539574 1105 86-87 0.5426235105823665 1105 88-89 0.4416624482367979 1105 90-91 0.545569838277089 1105 92-93 0.634666445922079 1105 94-95 0.7125048819469129 1105 96-97 0.6569188136298898 1105 98-99 0.7626678809111844 1105 100-101 0.5848005527492894 1106 1 0.1516889161780739 1106 2 0.15496349258339137 1106 3 0.203677528277332 1106 4 0.13531961531227665 1106 5 0.1786045695921672 1106 6 0.12560098442454404 1106 7 0.26595928294162263 1106 8 0.18560645841979095 1106 9 0.3242768503425211 1106 10-11 0.3206306874984648 1106 12-13 0.3309110537596993 1106 14-15 0.350945820896726 1106 16-17 0.37417075773391417 1106 18-19 0.3719265026084102 1106 20-21 0.3717801351513117 1106 22-23 0.48771975939704504 1106 24-25 0.4450923611665729 1106 26-27 0.5356121600934287 1106 28-29 0.4575929158884904 1106 30-31 0.3979832616280419 1106 32-33 0.3123200818462095 1106 34-35 0.3672696116181555 1106 36-37 0.20180244478034837 1106 38-39 0.7850425284342677 1106 40-41 0.7147479363145806 1106 42-43 0.5218865927528427 1106 44-45 0.45434175188744774 1106 46-47 0.34238500819013495 1106 48-49 0.24298770505276224 1106 50-51 0.2583465904557656 1106 52-53 0.20949078920703812 1106 54-55 0.24646678652731424 1106 56-57 0.45904362139385313 1106 58-59 0.42602290589278624 1106 60-61 0.44920807659848094 1106 62-63 0.3447733839574312 1106 64-65 0.29262309921109875 1106 66-67 0.26341393264317503 1106 68-69 0.4198821632780678 1106 70-71 0.4476729006249869 1106 72-73 0.4031015359701158 1106 74-75 0.34547022905462654 1106 76-77 0.4696978634139306 1106 78-79 0.584118454868257 1106 80-81 0.6437279669284912 1106 82-83 0.5211712861293307 1106 84-85 0.5064797307600095 1106 86-87 0.5705451049393027 1106 88-89 0.5432177861064638 1106 90-91 0.4832018874686632 1106 92-93 0.4264077810201172 1106 94-95 0.546875308115446 1106 96-97 0.7321148176914392 1106 98-99 0.6915260279090738 1106 100-101 0.4469475440597783 1107 1 0.15227016045349728 1107 2 0.15959228837605366 1107 3 0.17560591282742877 1107 4 0.19452574474649253 1107 5 0.1727386187908948 1107 6 0.21796968774270198 1107 7 0.21599570688343306 1107 8 0.19321911471661934 1107 9 0.14671616679645183 1107 10-11 0.2664544019151833 1107 12-13 0.3224601921577417 1107 14-15 0.33080869690878956 1107 16-17 0.3773328035319068 1107 18-19 0.34774716042165466 1107 20-21 0.3422340757391922 1107 22-23 0.2463165440880104 1107 24-25 0.4397813377744626 1107 26-27 0.3992288913952464 1107 28-29 0.38020786463793144 1107 30-31 0.39519629674785506 1107 32-33 0.40410762832038927 1107 34-35 0.48583181542226583 1107 36-37 0.4677675148671554 1107 38-39 0.9143022323634042 1107 40-41 0.6843551907467429 1107 42-43 0.307240198758052 1107 44-45 0.3141422752870753 1107 46-47 0.5672462807094192 1107 48-49 0.416400553209165 1107 50-51 0.45295387111735863 1107 52-53 0.5596797110828504 1107 54-55 0.5270858921175758 1107 56-57 0.43233383819937643 1107 58-59 0.38466389592455386 1107 60-61 0.3001972585303818 1107 62-63 0.2912127032091689 1107 64-65 0.23984685342660583 1107 66-67 0.2922967370803562 1107 68-69 0.4207000844869455 1107 70-71 0.3887882566301073 1107 72-73 0.31568731059352473 1107 74-75 0.2946255188496565 1107 76-77 0.25945880135053656 1107 78-79 0.4336006683387872 1107 80-81 0.47189378102195434 1107 82-83 0.5143497095864227 1107 84-85 0.5533611733649373 1107 86-87 0.6960608193434261 1107 88-89 0.7086482303666415 1107 90-91 0.7735380369106899 1107 92-93 0.7985768818366239 1107 94-95 0.7408112026773139 1107 96-97 0.8226214060008772 1107 98-99 0.9476119562369654 1107 100-101 0.8433633967105045 1108 1 0.1687984041684416 1108 2 0.30277176058054067 1108 3 0.2253414038836965 1108 4 0.15866729607956387 1108 5 0.18906550974122638 1108 6 0.14428442282630272 1108 7 0.2540425511117732 1108 8 0.2615150523481944 1108 9 0.35582661540905747 1108 10-11 0.345157187578053 1108 12-13 0.3643862179363282 1108 14-15 0.4200862377565713 1108 16-17 0.4168616108987706 1108 18-19 0.37914240265615007 1108 20-21 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2316 80-81 -1.5641582133837169 2316 82-83 -1.6437011411819284 2316 84-85 -1.453175095882937 2316 86-87 -1.2832058348026472 2316 88-89 -1.5580322494632952 2316 90-91 -1.5390280687698485 2316 92-93 -1.5900698265454096 2316 94-95 -1.5589177043474614 2316 96-97 -1.4948455993969638 2316 98-99 -1.8438597996543429 2316 100-101 -1.2111015346871703 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8120.0 3 1542.0 4 1513.0 5 1854.0 6 2257.0 7 2530.0 8 3256.0 9 4263.0 10 4876.0 11 6412.0 12 8640.0 13 11281.0 14 11540.0 15 11937.0 16 14359.0 17 17736.0 18 16297.0 19 12125.0 20 12400.0 21 13506.0 22 15038.0 23 16836.0 24 19689.0 25 22429.0 26 26475.0 27 30874.0 28 36207.0 29 42978.0 30 52009.0 31 62746.0 32 78724.0 33 100492.0 34 134733.0 35 192629.0 36 297126.0 37 488956.0 38 522202.0 39 85456.0 40 16.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.89958307947571 20.017174961958045 14.234462550741823 22.84877940782442 2 14.50545952579401 21.651386137369794 40.37686853160326 23.466285805232936 3 19.48494395500652 25.47402228061784 30.588353062098072 24.45268070227757 4 12.062787069573682 17.064437641073592 39.653178900166395 31.21959638918633 5 12.563559180006973 38.48166881184136 35.49007186335238 13.464700144799291 6 29.991123023345068 37.69923497286056 18.21236691336096 14.097275090433413 7 26.561853121780654 33.5249994034854 22.55478201682213 17.358365457911816 8 24.31840989312011 35.18169246942416 21.26628956500733 19.233608072448398 9 25.101872238627003 17.78708335793004 22.303415781499172 34.807628621943785 10-11 23.911417720432716 26.804333879969565 28.96292257475085 20.321325824846866 12-13 25.333498806978273 24.76451921360394 28.587002268426016 21.314979710991768 14-15 22.001690859832994 26.30972039273663 27.791054267223252 23.89753448020712 16-17 20.915008059961174 29.338907398653024 28.27126336621563 21.474821175170174 18-19 20.501502867474073 29.161469236902533 29.936065072413744 20.40096282320966 20-21 21.831569831450064 27.61473510620166 29.447557054000555 21.10613800834772 22-23 21.216701216575583 27.78524614192684 29.42948677680759 21.568565864689994 24-25 21.63186512485263 27.674446319432473 29.61048258170562 21.08320597400927 26-27 21.454239726436164 27.38643300104597 30.261533645273726 20.897793627244145 28-29 20.743492399860123 28.77763282707614 29.665024279824188 20.81385049323955 30-31 21.17829240522284 27.7627469118096 29.502325957298197 21.556634725669365 32-33 21.062882028587897 28.00129908827204 29.64911424752657 21.28670463561349 34-35 21.982221509900388 27.715023063284107 29.38938728108341 20.913368145732097 36-37 21.588913738609687 27.329605315114435 29.84307709369166 21.238403852584213 38-39 21.095559471635724 28.136966035434952 29.834049978804032 20.93342451412529 40-41 21.237636785008807 28.15950460434038 29.49017923368274 21.112679376968075 42-43 20.927959271613016 28.192089849738057 28.870217325019887 22.00973355362904 44-45 21.445770532454016 27.686651227373417 28.95266487409584 21.91491336607673 46-47 21.80895288557355 28.187655472147057 28.774777101899424 21.22861454037997 48-49 21.624991283497625 27.939603670088577 28.915368852975497 21.5200361934383 50-51 20.720185071034557 28.32445817052284 29.430578935043467 21.524777823399134 52-53 21.426958657706564 28.65200410408928 28.373938402954774 21.547098835249383 54-55 20.976991339255 27.912055281601262 28.861625058326997 22.24932832081674 56-57 21.553910543809703 27.963676606849795 28.60797831629708 21.87443453304342 58-59 22.101905721025144 27.693677499629537 28.574397086992683 21.630019692352644 60-61 21.525092312856664 27.938779791876467 29.29984894259819 21.23627895266868 62-63 21.047712539374704 28.007231629305068 29.221961369422587 21.723094461897638 64-65 20.834428087021628 28.529669662668844 28.788210054091856 21.847692196217665 66-67 21.207383747027077 27.940096902070184 28.244094747850664 22.608424603052075 68-69 20.860902041623156 27.943762460930106 28.701034098335448 22.49430139911129 70-71 21.535365671782873 27.616059856869484 28.609949398091455 22.238625073256188 72-73 21.306235441639483 27.13634291883445 29.328734869281938 22.22868677024413 74-75 20.76738719929088 28.447602623912218 28.514208656893864 22.270801519903042 76-77 20.944867437959882 27.54845222312918 28.61568106739264 22.890999271518297 78-79 20.973533326432385 27.604951968775243 28.78301930172629 22.638495403066077 80-81 21.3375501965028 27.408266849081066 28.600385237031634 22.653797717384503 82-83 21.137273941012257 27.182368774387662 29.145644963967893 22.534712320632185 84-85 20.879407484560286 27.496634866770126 28.88999951701676 22.73395813165283 86-87 20.767848836781987 27.521516672074053 28.830776112162837 22.879858378981123 88-89 20.839148884695206 27.275919438145284 28.453324451273065 23.431607225886445 90-91 20.962825419364055 26.728372241523445 28.753229512810925 23.55557282630157 92-93 21.365835501152812 27.034368341097153 28.81800903426163 22.78178712348841 94-95 21.050693532595602 26.679804992073525 28.923311992306893 23.34618948302398 96-97 20.451095822100783 26.64316600983297 29.424574192380227 23.48116397568602 98-99 20.89842072086813 26.840960729013748 28.519823064948792 23.74079548516933 100-101 20.77754725868841 25.876800322236704 29.165193983598776 24.18045843547611 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2994.0 1 1738.0 2 522.5 3 558.0 4 511.5 5 523.0 6 746.0 7 1264.5 8 1952.5 9 2324.0 10 2141.0 11 2035.0 12 2303.0 13 2562.0 14 2723.0 15 2824.5 16 2949.5 17 3155.5 18 3369.5 19 3513.0 20 3815.0 21 4474.5 22 5410.5 23 6772.5 24 8438.0 25 10393.0 26 13811.5 27 18137.5 28 21919.5 29 26612.0 30 32533.0 31 38172.0 32 44112.5 33 50883.0 34 58488.5 35 65050.5 36 71984.5 37 78830.5 38 80711.5 39 81328.0 40 82945.0 41 85090.5 42 87502.5 43 89451.0 44 91174.0 45 92195.0 46 92910.0 47 102361.0 48 124356.0 49 121521.0 50 95362.5 51 78772.0 52 68233.5 53 61082.5 54 55325.5 55 49054.5 56 42948.5 57 36921.0 58 31190.0 59 26259.0 60 21846.0 61 17928.0 62 14646.0 63 11812.5 64 9441.0 65 7689.5 66 6420.0 67 5456.5 68 4523.5 69 3658.5 70 2938.0 71 2372.0 72 1898.0 73 1486.5 74 1208.5 75 926.5 76 633.0 77 447.5 78 313.0 79 203.5 80 136.5 81 81.0 82 54.0 83 45.0 84 34.5 85 27.5 86 21.0 87 11.5 88 9.0 89 10.0 90 8.0 91 5.0 92 4.0 93 4.0 94 3.0 95 3.5 96 4.0 97 2.0 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.30066148033974077 2 0.1876625952787954 3 0.1886659150129658 4 0.2093175795413073 5 0.22185907621843776 6 0.11421122973973467 7 0.13302347475543036 8 0.14589941134395096 9 0.14748800092305414 10-11 0.1340476969840627 12-13 0.12363825474204442 14-15 0.13935693057738124 16-17 0.11619696671361365 18-19 0.14458255419285226 20-21 0.1714840645652971 22-23 0.1759571983801403 24-25 0.2000159695057689 26-27 0.18045123468944538 28-29 0.17867452266018521 30-31 0.1676380055843104 32-33 0.17685600564200132 34-35 0.36023358955611046 36-37 0.4818861073242759 38-39 0.4477105288790954 40-41 0.4367576217810681 42-43 0.46056556297315404 44-45 0.46491328182122593 46-47 0.4684876083742082 48-49 0.4814680574350382 50-51 0.4745493317681545 52-53 0.48102910505133867 54-55 0.46345010720889407 56-57 0.42549117726611263 58-59 0.41453827016808537 60-61 0.37035039687566235 62-63 0.4768277036644999 64-65 0.4760125063804865 66-67 0.4855440438551056 68-69 0.4962043160306665 70-71 0.4897036402530205 72-73 0.4830148420252176 74-75 0.48677729102835676 76-77 0.4631783747808896 78-79 0.44913189850250346 80-81 0.445975621838759 82-83 0.4593532182943648 84-85 0.4608581978956205 86-87 0.4622168600356429 88-89 0.46947002561391665 90-91 0.48782241575145097 92-93 0.49275540444445565 94-95 0.4910204974041192 96-97 0.4891183704080877 98-99 0.4865055586003523 100-101 0.4682367784406656 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2392059.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.92884527879523 #Duplication Level Percentage of deduplicated Percentage of total 1 69.03763753130158 32.39856610119999 2 14.770221237598783 13.862988543856972 3 6.206053185646346 8.737287292235148 4 3.3228926368621243 6.23758057733402 5 1.9325992009657702 4.534732444402295 6 1.2112036730346456 3.4104233863770816 7 0.8094374113187147 2.659017412705115 8 0.5599070539783346 2.102063320532425 9 0.39629044910313505 1.6737707854282766 >10 1.6086375735134235 12.74394651778249 >50 0.09657593628919281 3.1466797516162064 >100 0.04487602546542016 3.68279270018719 >500 0.0013419822886443047 0.4058701498241184 >1k 0.0018787752041012368 2.410911514776296 >5k 3.5786194363833084E-4 1.2655994410152145 >10k+ 8.946548590958271E-5 0.7277700607271008 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 17334 0.7246476780045977 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 9201 0.3846477030875911 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 7689 0.3214385598348536 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6733 0.2814729904237312 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 6521 0.27261033277189234 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 4789 0.2002040919559258 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4711 0.1969433028198719 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 4352 0.18193531179623915 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 4190 0.1751629035905887 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3843 0.16065657243404113 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 3660 0.15300625946099156 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3401 0.14217876732973558 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 3396 0.14196974238511675 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 3154 0.13185293506556486 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2620 0.10952907098027265 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 2.0902494461884094E-5 0.0 0.0 0.0 0.0 14-15 4.180498892376819E-5 0.0 0.0 0.0 0.0 16-17 4.180498892376819E-5 0.0 0.0 4.180498892376819E-5 0.0 18-19 4.180498892376819E-5 0.0 0.0 8.360997784753637E-5 0.0 20-21 8.360997784753637E-5 0.0 0.0 2.717324280044932E-4 0.0 22-23 8.360997784753637E-5 0.0 0.0 5.434648560089864E-4 0.0 24-25 1.2541496677130455E-4 0.0 0.0 0.0010033197341704364 2.0902494461884094E-5 26-27 1.2541496677130455E-4 0.0 0.0 0.0014422721178700025 4.180498892376819E-5 28-29 1.6721995569507275E-4 0.0 0.0 0.004473133814843196 4.180498892376819E-5 30-31 1.6721995569507275E-4 0.0 0.0 0.016784703052892924 4.180498892376819E-5 32-33 1.6721995569507275E-4 0.0 0.0 0.04330996852502384 4.180498892376819E-5 34-35 1.6721995569507275E-4 0.0 0.0 0.08478051753740187 4.180498892376819E-5 36-37 1.6721995569507275E-4 0.0 0.0 0.15311077193330097 4.180498892376819E-5 38-39 1.6721995569507275E-4 0.0 0.0 0.2578113666928784 4.180498892376819E-5 40-41 1.6721995569507275E-4 0.0 0.0 0.3740501383954158 4.180498892376819E-5 42-43 1.6721995569507275E-4 0.0 0.0 0.4943857990124826 4.180498892376819E-5 44-45 1.6721995569507275E-4 0.0 0.0 0.6393655006001107 4.180498892376819E-5 46-47 1.6721995569507275E-4 0.0 0.0 0.8154063089580984 4.180498892376819E-5 48-49 1.6721995569507275E-4 0.0 0.0 0.9987420878832838 4.180498892376819E-5 50-51 1.6721995569507275E-4 0.0 0.0 1.192027454172326 4.180498892376819E-5 52-53 1.6721995569507275E-4 0.0 0.0 1.3903085166377585 4.180498892376819E-5 54-55 1.6721995569507275E-4 0.0 0.0 1.6150730395863981 4.180498892376819E-5 56-57 2.0902494461884092E-4 0.0 0.0 1.8958353451984253 4.180498892376819E-5 58-59 2.0902494461884092E-4 0.0 0.0 2.1949500409479867 4.180498892376819E-5 60-61 2.0902494461884092E-4 0.0 0.0 2.5160332583769884 4.180498892376819E-5 62-63 2.0902494461884092E-4 0.0 0.0 2.8402100449863488 4.180498892376819E-5 64-65 2.0902494461884092E-4 0.0 4.180498892376819E-5 3.193942958764813 6.270748338565229E-5 66-67 2.0902494461884092E-4 0.0 4.180498892376819E-5 3.600412866070611 8.360997784753637E-5 68-69 2.0902494461884092E-4 0.0 4.180498892376819E-5 4.0580520798191015 8.360997784753637E-5 70-71 2.0902494461884092E-4 0.0 4.180498892376819E-5 4.549783262034925 8.360997784753637E-5 72-73 2.0902494461884092E-4 0.0 4.180498892376819E-5 5.037689287764223 8.360997784753637E-5 74-75 2.0902494461884092E-4 0.0 4.180498892376819E-5 5.567483912395137 8.360997784753637E-5 76-77 2.0902494461884092E-4 0.0 4.180498892376819E-5 6.125580514527442 8.360997784753637E-5 78-79 2.0902494461884092E-4 0.0 4.180498892376819E-5 6.787186269234998 8.360997784753637E-5 80-81 2.0902494461884092E-4 0.0 4.180498892376819E-5 7.497473933544281 8.360997784753637E-5 82-83 2.0902494461884092E-4 0.0 4.180498892376819E-5 8.19994406492482 8.360997784753637E-5 84-85 2.508299335426091E-4 0.0 4.180498892376819E-5 8.92505159780758 8.360997784753637E-5 86-87 2.9263492246637727E-4 0.0 4.180498892376819E-5 9.682035434744712 8.360997784753637E-5 88-89 2.9263492246637727E-4 0.0 4.180498892376819E-5 10.507788478461443 8.360997784753637E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 8040 0.0 27.859205 1 TACATGG 7885 0.0 27.779377 2 ACATGGG 8290 0.0 25.964716 3 CATGGGG 3375 0.0 23.459833 4 GTACCGT 190 3.371497E-7 22.442955 6 GAGTACT 6710 0.0 20.19457 12-13 AGTACTT 7260 0.0 19.447813 12-13 CATGGGA 4935 0.0 19.310394 4 AGAGTAC 11255 0.0 18.583643 10-11 GTACTTT 7550 0.0 17.95114 14-15 GTATAGC 1045 0.0 17.71051 1 CCTAGAC 645 0.0 17.641428 3 ATGGGAT 2350 0.0 16.947031 5 CCTATAC 645 0.0 16.906368 3 ATAGCCC 915 0.0 16.581015 3 TGTACCG 300 1.4964753E-6 15.803779 5 TAGCCCT 2105 0.0 15.766241 4 GTATAGA 865 0.0 15.361185 1 AGTACAT 5990 0.0 15.355258 2 GAGTACA 6650 0.0 15.342623 1 >>END_MODULE