FastQCFastQC Report
Fri 27 May 2016
SRR939301_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939301_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2392059
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT181960.7606835784568858No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT90230.3772064150591603No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT73580.3076011085010863No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66370.27745971148704945No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC64880.271230768137408No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49450.20672567022803368No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT47830.19995326202238323No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT43230.18072296711744987No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC43110.18022130725036464No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC41000.17140045458744957No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG40140.16780522554000551No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35780.14957825036924255No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC34750.14527233651009444No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA34280.14330750203067735No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA27830.11634328417484685No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG79450.025.767551
TACATGG78050.025.3548742
ACATGGG81550.023.4519733
GAGTACT66800.021.59755112-13
CATGGGG35400.020.3769254
GTAAGCG2900.019.63729194-95
AGAGTAC114350.019.50583510-11
AGTACTT75550.019.22181312-13
GTACTTT77250.018.79881114-15
GTGTAGC14550.018.3241391
AGTACAT60900.018.2346272
CATGGGA45350.018.2083364
GAGTACA67450.018.1405181
ATAAGCG1800.00263567315.8189283
TACTTTT93100.015.77677914-15
TGTATCG2751.1385942E-515.5313119
GTATAGG5900.015.33204651
ACTTTTT97300.015.12015416-17
ACCGCTC1900.003778308414.9863538
TAATACA12100.014.5115794
Produced by FastQC (version 0.11.5)