##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939301_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2392059 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.317040256950186 33.0 31.0 34.0 31.0 34.0 2 32.52488003013303 34.0 31.0 34.0 31.0 34.0 3 32.62539051085278 34.0 31.0 34.0 31.0 34.0 4 36.13393900401286 37.0 35.0 37.0 35.0 37.0 5 36.07788520266431 37.0 35.0 37.0 35.0 37.0 6 36.107164580806746 37.0 35.0 37.0 35.0 37.0 7 36.05037292140369 37.0 35.0 37.0 35.0 37.0 8 36.03299667775753 37.0 35.0 37.0 35.0 37.0 9 37.7458486600874 39.0 38.0 39.0 35.0 39.0 10-11 37.67050436464987 39.0 37.0 39.0 35.0 39.0 12-13 37.61466753119384 39.0 37.0 39.0 35.0 39.0 14-15 38.91463567579228 40.0 38.0 41.0 35.0 41.0 16-17 38.9255965258382 40.0 38.0 41.0 35.0 41.0 18-19 39.02802940897361 40.0 38.5 41.0 35.0 41.0 20-21 39.01028110092602 40.0 38.5 41.0 35.0 41.0 22-23 38.93721559543472 40.0 38.0 41.0 35.0 41.0 24-25 38.8788771514415 40.0 38.0 41.0 35.0 41.0 26-27 38.7318780598639 40.0 38.0 41.0 34.5 41.0 28-29 38.59417326244879 40.0 38.0 41.0 34.0 41.0 30-31 38.4358124109815 40.0 38.0 41.0 34.0 41.0 32-33 38.38384170290114 40.0 38.0 41.0 34.0 41.0 34-35 38.28376181356731 40.0 38.0 41.0 34.0 41.0 36-37 38.14393102344047 40.0 38.0 41.0 33.5 41.0 38-39 38.02684653681201 40.0 38.0 41.0 33.0 41.0 40-41 37.8972813797653 40.0 38.0 41.0 33.0 41.0 42-43 37.73527743253824 40.0 37.0 41.0 33.0 41.0 44-45 37.51811389267573 40.0 37.0 41.0 32.5 41.0 46-47 37.26670077953763 40.0 37.0 41.0 32.0 41.0 48-49 37.003030234622145 40.0 37.0 41.0 31.0 41.0 50-51 36.79159711361635 40.0 36.0 41.0 31.0 41.0 52-53 36.59421862086178 39.0 36.0 41.0 30.5 41.0 54-55 36.393899983236196 39.0 36.0 41.0 30.0 41.0 56-57 36.1035810571562 39.0 35.0 40.0 29.5 41.0 58-59 35.82850464808769 38.5 35.0 40.0 29.0 41.0 60-61 35.4998796016319 38.0 35.0 40.0 28.0 41.0 62-63 35.21687424933917 38.0 34.0 40.0 28.0 41.0 64-65 34.96759298161124 38.0 34.0 40.0 28.0 41.0 66-67 34.56926292369879 37.0 34.0 39.5 26.5 41.0 68-69 34.11841221307668 36.5 34.0 39.0 26.0 40.5 70-71 33.65092332588786 36.0 33.0 39.0 26.0 40.0 72-73 33.16324973589698 35.5 32.5 38.5 25.5 40.0 74-75 32.65630613626169 35.0 32.0 37.5 24.5 39.5 76-77 30.637930753380246 33.5 29.5 35.5 21.0 38.0 78-79 31.60889990589697 34.5 31.0 36.0 22.0 39.0 80-81 31.596709780151745 35.0 31.0 36.0 23.0 38.0 82-83 31.239910679460664 34.0 31.0 35.5 21.5 37.0 84-85 30.79292274981512 34.0 31.0 35.0 20.0 37.0 86-87 30.236637139802987 34.0 30.5 35.0 17.5 36.0 88-89 29.65845261341798 34.0 30.0 35.0 9.5 36.0 90-91 29.19588521855021 34.0 29.0 35.0 4.5 35.5 92-93 28.87405013839542 34.0 29.0 35.0 2.0 35.0 94-95 28.39741076620602 33.5 29.0 35.0 2.0 35.0 96-97 27.731104458543875 33.0 28.0 35.0 2.0 35.0 98-99 26.849614704319585 33.0 26.5 34.5 2.0 35.0 100-101 22.667581359824318 28.5 11.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.24044728395049475 1101 2 0.2636799731905981 1101 3 0.2649446134802744 1101 4 0.19363687494273307 1101 5 0.16087505198809993 1101 6 0.15803210546315682 1101 7 0.23299460206097677 1101 8 0.23440923970502325 1101 9 0.34733868087483444 1101 10-11 0.23689945641095989 1101 12-13 0.24614463086894034 1101 14-15 0.27130231554378526 1101 16-17 0.2429875348587487 1101 18-19 0.35497127498589975 1101 20-21 0.24056517480598671 1101 22-23 0.27530253888513556 1101 24-25 0.2566043536689122 1101 26-27 0.22935962562780077 1101 28-29 0.30922453436202346 1101 30-31 0.29997114905284405 1101 32-33 0.4014277498125338 1101 34-35 0.4232358616313121 1101 36-37 0.37392090550196144 1101 38-39 0.4721833569734386 1101 40-41 0.5307167587819706 1101 42-43 0.6155440213734025 1101 44-45 0.5872488356394783 1101 46-47 0.5548309681599619 1101 48-49 0.5493762040608132 1101 50-51 0.6041417923317169 1101 52-53 0.5255894904308818 1101 54-55 0.36810920482211174 1101 56-57 0.43340361344105105 1101 58-59 0.378482290286108 1101 60-61 0.39551647312719496 1101 62-63 0.4202759201331716 1101 64-65 0.43484604821758666 1101 66-67 0.3276615477154934 1101 68-69 0.4107606854998025 1101 70-71 0.47834527474369537 1101 72-73 0.44832909969522206 1101 74-75 0.4538938577032283 1101 76-77 0.39354148703725045 1101 78-79 0.5284673769776731 1101 80-81 0.6556411848475534 1101 82-83 0.6418498949247571 1101 84-85 0.5792428647435983 1101 86-87 0.5345321187558199 1101 88-89 0.7771738605443126 1101 90-91 0.8926214831731052 1101 92-93 0.7843583004362706 1101 94-95 0.5636139310480992 1101 96-97 0.5399627565773564 1101 98-99 0.3954543221294138 1101 100-101 0.5882893123288468 1102 1 0.15075857572200846 1102 2 0.21106759398242758 1102 3 0.22113198412692725 1102 4 0.1105206815169879 1102 5 0.19833602884470736 1102 6 0.20904509415203165 1102 7 0.21582690056754927 1102 8 0.2022861821766 1102 9 0.31385942952360324 1102 10-11 0.26909261910954996 1102 12-13 0.2478377017483666 1102 14-15 0.32068427332682603 1102 16-17 0.35690037117745277 1102 18-19 0.34819216707109035 1102 20-21 0.2693448981573283 1102 22-23 0.3418683285547388 1102 24-25 0.31360419980978094 1102 26-27 0.35335643925476035 1102 28-29 0.30712448125581204 1102 30-31 0.34100935078698313 1102 32-33 0.3610422086006153 1102 34-35 0.3081237926230145 1102 36-37 0.44598715923056886 1102 38-39 0.4768766809759626 1102 40-41 0.5205924554908634 1102 42-43 0.5086913340499066 1102 44-45 0.48694632872822297 1102 46-47 0.5064162135652737 1102 48-49 0.47262158952757005 1102 50-51 0.4367486375742473 1102 52-53 0.47997459984458146 1102 54-55 0.4783294219123846 1102 56-57 0.41936396741632365 1102 58-59 0.4109875140518895 1102 60-61 0.45380550991938406 1102 62-63 0.6861370597826735 1102 64-65 0.6800249218694674 1102 66-67 0.6228148608982451 1102 68-69 0.5906735069289013 1102 70-71 0.39251669367234143 1102 72-73 0.5123698624394706 1102 74-75 0.5029544484230399 1102 76-77 0.4550876224432123 1102 78-79 0.5258796896789875 1102 80-81 0.6404823376991544 1102 82-83 0.5657392553174461 1102 84-85 0.4579931811474651 1102 86-87 0.2992957712724085 1102 88-89 0.36824233778556703 1102 90-91 0.4762246903915788 1102 92-93 0.3746357680141834 1102 94-95 0.564059998458255 1102 96-97 0.08407379820369698 1102 98-99 -0.29126490480288325 1102 100-101 -0.2659539737052121 1103 1 0.10722255839210959 1103 2 0.12427726724980204 1103 3 0.1545153449024994 1103 4 0.15478889660584372 1103 5 0.14167387921894203 1103 6 0.09281143199754638 1103 7 0.11382070299315217 1103 8 0.1324129021734919 1103 9 0.15938391625661552 1103 10-11 0.11261904379882282 1103 12-13 0.18523782900263086 1103 14-15 0.2353899128281043 1103 16-17 0.1365653087402876 1103 18-19 0.10010462390420827 1103 20-21 0.21872186222014278 1103 22-23 0.19737159005254767 1103 24-25 0.17376535617224675 1103 26-27 0.17536134971535233 1103 28-29 0.20494889464761457 1103 30-31 0.10675767389626145 1103 32-33 0.2080535546302329 1103 34-35 0.23281063540570557 1103 36-37 0.25316294012008456 1103 38-39 0.3499362962017898 1103 40-41 0.43051634578019815 1103 42-43 0.4059553672331546 1103 44-45 0.35220919136646245 1103 46-47 0.3853236967963696 1103 48-49 0.25788365075533903 1103 50-51 0.26555096603597406 1103 52-53 0.2216447878603347 1103 54-55 0.2652301247250932 1103 56-57 0.25723498404195055 1103 58-59 0.13031474209444838 1103 60-61 0.10688887603769359 1103 62-63 0.13681328023368877 1103 64-65 0.3116602363866505 1103 66-67 0.24496135387740026 1103 68-69 0.25717078456178655 1103 70-71 0.16129701977354927 1103 72-73 0.2643460678336993 1103 74-75 0.0814568550830046 1103 76-77 0.16100883165020718 1103 78-79 0.3169768691997916 1103 80-81 0.5226027484098879 1103 82-83 0.41067118399677227 1103 84-85 0.37851451080888054 1103 86-87 0.4432114871748247 1103 88-89 0.5667292505095354 1103 90-91 0.6382783897509405 1103 92-93 0.32617107621722496 1103 94-95 0.16292673118057266 1103 96-97 0.0765314327313753 1103 98-99 -0.14320249678295127 1103 100-101 -0.20665263364974606 1104 1 0.0909648459660275 1104 2 0.08141124677989353 1104 3 0.12913471725527614 1104 4 0.10829882434674687 1104 5 0.12006273267525103 1104 6 0.09517684855616437 1104 7 0.09802264064894928 1104 8 0.09946294153869673 1104 9 0.10086409464990709 1104 10-11 0.1296626001704979 1104 12-13 0.13748853745949674 1104 14-15 0.20859178740612805 1104 16-17 0.21369920650289487 1104 18-19 0.213368945479381 1104 20-21 0.22015483741189001 1104 22-23 0.24317364732250013 1104 24-25 0.16604592144256003 1104 26-27 0.18966335909291843 1104 28-29 0.19315773114301749 1104 30-31 0.17816025494536092 1104 32-33 0.1397855885614021 1104 34-35 0.23761330709990602 1104 36-37 0.22219233669940053 1104 38-39 0.2919465031202577 1104 40-41 0.41088163618326945 1104 42-43 0.31672835032130564 1104 44-45 0.3437255226696294 1104 46-47 0.3834923971372248 1104 48-49 0.42575814000057477 1104 50-51 0.3580536468414053 1104 52-53 0.3776507265160376 1104 54-55 0.5376925292031487 1104 56-57 0.35741533975387796 1104 58-59 0.34284917204658427 1104 60-61 0.3555265713159699 1104 62-63 0.44402277787762046 1104 64-65 0.4507700921721707 1104 66-67 0.35087156616916104 1104 68-69 0.4637770717805907 1104 70-71 0.3687274064403141 1104 72-73 0.3228486598629985 1104 74-75 0.3551551167062499 1104 76-77 0.4980111888900147 1104 78-79 0.5421981086547092 1104 80-81 0.48148801227356586 1104 82-83 0.49321195676959917 1104 84-85 0.5451131352461474 1104 86-87 0.4898174940479656 1104 88-89 0.7164522339481181 1104 90-91 0.8021719705360475 1104 92-93 0.719159631105402 1104 94-95 0.652178687175649 1104 96-97 0.40477444803591567 1104 98-99 0.6157846260061177 1104 100-101 0.24384737452700378 1105 1 0.13487454597995452 1105 2 0.14295065061124745 1105 3 0.09794099807946566 1105 4 0.06695912640658719 1105 5 0.13233352374627572 1105 6 0.1485137963389036 1105 7 0.13452517690733856 1105 8 0.0019325443405762144 1105 9 0.12240352872770188 1105 10-11 0.21113676999554798 1105 12-13 0.22059554236452072 1105 14-15 0.2229821921771986 1105 16-17 0.20823688874477853 1105 18-19 0.17841181318958377 1105 20-21 0.18293423179878943 1105 22-23 0.23494879000534752 1105 24-25 0.20082554494623395 1105 26-27 0.29785184506773277 1105 28-29 0.28551211202772464 1105 30-31 0.31671021043583636 1105 32-33 0.23635991474164797 1105 34-35 0.34023937850909647 1105 36-37 0.3153126141197333 1105 38-39 0.30443421786760183 1105 40-41 0.38617057946533606 1105 42-43 0.4295274332492838 1105 44-45 0.3639253230809416 1105 46-47 0.45317452030110417 1105 48-49 0.49167599936938444 1105 50-51 0.588638505209623 1105 52-53 0.6146880954237162 1105 54-55 0.5384226026131458 1105 56-57 0.5422483009614965 1105 58-59 0.41059665738604423 1105 60-61 0.45642320808214265 1105 62-63 0.5552475336801663 1105 64-65 0.6147931255912695 1105 66-67 0.6724021720677058 1105 68-69 0.7271333791743828 1105 70-71 0.6834244911940459 1105 72-73 0.5853579724578566 1105 74-75 0.53824503237351 1105 76-77 0.5319612092969095 1105 78-79 0.5817069518626568 1105 80-81 0.7177229053590253 1105 82-83 0.6538578808068074 1105 84-85 0.7623845510951668 1105 86-87 0.7908497104748413 1105 88-89 0.8806478611070752 1105 90-91 0.9911243076310932 1105 92-93 1.0168668187367267 1105 94-95 0.986010992224589 1105 96-97 0.9972135189484987 1105 98-99 1.006343559395706 1105 100-101 0.8805489890629872 1106 1 0.061992616382688936 1106 2 0.12990514486386928 1106 3 0.16706604997673224 1106 4 0.0998011199993627 1106 5 0.08507719702942751 1106 6 0.13729156719602997 1106 7 0.15701548196003756 1106 8 0.09509938510927185 1106 9 0.14354053220127838 1106 10-11 0.1994263001830845 1106 12-13 0.21975998047038559 1106 14-15 0.20804491365955613 1106 16-17 0.22312256681755116 1106 18-19 0.18084899809581145 1106 20-21 0.27141648279832964 1106 22-23 0.38441829176789355 1106 24-25 0.28554507323962497 1106 26-27 0.2332353934577256 1106 28-29 0.34081262845228366 1106 30-31 0.3140656191396758 1106 32-33 0.2719982871339326 1106 34-35 0.32294866306391157 1106 36-37 0.33848760684544743 1106 38-39 0.4484323372368806 1106 40-41 0.5125436307722495 1106 42-43 0.4412015544297816 1106 44-45 0.517821595044694 1106 46-47 0.4168749315886089 1106 48-49 0.3485275064402842 1106 50-51 0.49588565526682515 1106 52-53 0.49995911831257445 1106 54-55 0.37602047678441153 1106 56-57 0.5482307560796116 1106 58-59 0.5314817958916791 1106 60-61 0.6482233402888724 1106 62-63 0.5308878312713503 1106 64-65 0.4813080717286269 1106 66-67 0.5728405292629688 1106 68-69 0.6580921665840265 1106 70-71 0.4941005410439132 1106 72-73 0.5269186877331862 1106 74-75 0.4509696838147903 1106 76-77 0.4073837621435281 1106 78-79 0.6601495828533501 1106 80-81 0.6606220357134376 1106 82-83 0.6117356613629887 1106 84-85 0.6827266154673133 1106 86-87 0.8370264462909773 1106 88-89 0.9970748125034739 1106 90-91 0.995780859636394 1106 92-93 1.1174693292764601 1106 94-95 1.259683130121438 1106 96-97 1.0317965024186364 1106 98-99 1.2056103216404033 1106 100-101 1.3383904209872064 1107 1 0.03706363694901427 1107 2 0.05145710205184173 1107 3 0.08269942263090257 1107 4 0.06958516082215738 1107 5 0.158846433259761 1107 6 0.1399480843537546 1107 7 0.09773398661646127 1107 8 0.0802926942952027 1107 9 0.10491629053795748 1107 10-11 0.13898983539331056 1107 12-13 0.11750164361939142 1107 14-15 0.1361267760753222 1107 16-17 0.17720890292572022 1107 18-19 0.15273322976396742 1107 20-21 0.17320141332355377 1107 22-23 0.14163610763935708 1107 24-25 0.14213170399178665 1107 26-27 0.09700215712666704 1107 28-29 0.24217806288341137 1107 30-31 0.20619969142030214 1107 32-33 0.11721664368148055 1107 34-35 0.2614064527339224 1107 36-37 0.2619626187623467 1107 38-39 0.12856035786230535 1107 40-41 0.17611650645907417 1107 42-43 0.14156331079470164 1107 44-45 0.17094020489863482 1107 46-47 0.1618576778663936 1107 48-49 0.18663261512981677 1107 50-51 0.18928813055607208 1107 52-53 0.18308813600624774 1107 54-55 0.1758054303915344 1107 56-57 0.24156879439067325 1107 58-59 0.2323677912270412 1107 60-61 0.19286776183016485 1107 62-63 0.16012113090277325 1107 64-65 0.10093868784405657 1107 66-67 0.19967313615753568 1107 68-69 0.36848169817178444 1107 70-71 0.4051742761818744 1107 72-73 0.3480026418589546 1107 74-75 0.31276224535691455 1107 76-77 0.31782764112021766 1107 78-79 0.36565440679613914 1107 80-81 0.3642995423643498 1107 82-83 0.43397589640670375 1107 84-85 0.4288553257520178 1107 86-87 0.47350510270041823 1107 88-89 0.6857037929513545 1107 90-91 0.5256337160043607 1107 92-93 0.6804114919737962 1107 94-95 0.9285081878349395 1107 96-97 0.9178202529969965 1107 98-99 1.0227706146126998 1107 100-101 0.8477804712183215 1108 1 0.01950010726561402 1108 2 0.07460893493922782 1108 3 0.026146799272623866 1108 4 0.11485727567133353 1108 5 0.05454536164201329 1108 6 0.06832354444312472 1108 7 0.0828579900391162 1108 8 0.055902864477339165 1108 9 0.11277583934477775 1108 10-11 0.10814262580986878 1108 12-13 0.14967377476693144 1108 14-15 0.13635240661846382 1108 16-17 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-1.340067249460514 2316 92-93 -1.2461699295338171 2316 94-95 -1.0338273093998716 2316 96-97 -0.9111731956858975 2316 98-99 -0.7369462512307159 2316 100-101 -0.08429529996276841 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 6.0 4 18.0 5 24.0 6 82.0 7 232.0 8 546.0 9 1045.0 10 1875.0 11 2899.0 12 4381.0 13 5903.0 14 7393.0 15 9458.0 16 11892.0 17 16404.0 18 15728.0 19 11310.0 20 10789.0 21 11494.0 22 12383.0 23 14176.0 24 16025.0 25 19761.0 26 23055.0 27 28719.0 28 35003.0 29 42735.0 30 52998.0 31 65406.0 32 81098.0 33 103709.0 34 140507.0 35 203089.0 36 324213.0 37 562529.0 38 513989.0 39 41179.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.8254456440659 20.683847124367745 14.742387477310551 22.748319754255807 2 14.827769716382413 22.18762162638965 39.91678298904835 23.06782566817959 3 19.511517065423554 25.621776051510437 30.82850381198792 24.038203071078097 4 12.303041020309282 17.494969814707748 39.35036719412021 30.85162197086276 5 12.490201955720991 38.78466208400378 35.413758607124656 13.311377353150569 6 29.88245691264304 38.129285272645866 17.989731858620544 13.99852595609055 7 26.524220347407816 33.600676237500835 22.422565664141228 17.45253775095012 8 24.35011009343833 35.64109413689211 20.96018534659889 19.04861042307067 9 24.88605005144104 17.82493659228305 22.253673508889204 35.03533984738671 10-11 23.977606739633096 27.016892141874425 28.795297273185987 20.21020384530649 12-13 25.319442371613743 24.832539665618615 28.541896332824567 21.30612162994307 14-15 21.883615225191235 26.5172463887777 27.716984267497484 23.882154118533585 16-17 20.792875371466327 29.547074976364325 28.207799993352957 21.452249658816395 18-19 20.515744183460434 29.24347988217677 29.87371125616519 20.367064678197604 20-21 21.86177264022334 27.618277810037295 29.433345916635 21.086603633104367 22-23 21.116410590207014 27.881816460212725 29.42757682816352 21.57419612141674 24-25 21.60895696970685 27.78533054577667 29.608864162631438 20.996848321885036 26-27 21.435637966211946 27.483483367986196 30.283393988901192 20.79748467690067 28-29 20.71033783029599 28.801839753952557 29.755955016159717 20.73186739959173 30-31 21.277986872397378 27.7470371759225 29.509619118926416 21.465356832753706 32-33 21.047390553493873 28.03691296911991 29.689046131387226 21.22665034599899 34-35 21.988250553139544 27.703255919925667 29.462286700299224 20.846206826635562 36-37 21.56579270555158 27.38640508803194 29.860413585505167 21.18738862091131 38-39 21.039709096503508 28.204353184537094 29.87854076679058 20.877396952168816 40-41 21.24718591462739 28.182597298896106 29.481539390924272 21.08867739555223 42-43 21.047643573263812 28.12984155039978 28.890422206187655 21.93209267014875 44-45 21.464029887528795 27.751355592906375 28.933926329239817 21.850688190325013 46-47 21.847302842116925 28.231855036778004 28.736337584211064 21.184504536894007 48-49 21.646804697476078 28.005491522858733 28.879532434620426 21.468171345044766 50-51 20.763957068775703 28.33676517526641 29.38724115651854 21.51203659943935 52-53 21.44714330654677 28.67276392812464 28.323023171474766 21.557069593853825 54-55 21.01298504760961 27.955330533235173 28.781773359269145 22.249911059886067 56-57 21.575799102315656 28.03640485634981 28.571052324090264 21.81674371724427 58-59 22.14664430056281 27.74598794947769 28.515319213423844 21.592048536535657 60-61 21.513122442828255 28.13333099224221 29.084232040591175 21.26931452433836 62-63 20.99258142280915 28.158089627014316 29.072833576257544 21.77649537391899 64-65 20.795018182038607 28.668466929215985 28.610462494959886 21.92605239378552 66-67 21.204468970130222 28.122928032440793 28.041115361300978 22.631487636128007 68-69 20.865362486577805 28.108865014664342 28.503154124755724 22.522618374002125 70-71 21.49259278303754 27.860307793411447 28.378313411165863 22.268786012385146 72-73 21.33822786143653 27.423236634213456 28.986116145128527 22.25241935922149 74-75 20.783467788713274 28.70863263111359 28.151350876807935 22.3565487033652 76-77 20.9480052148397 27.78978940086555 28.325146638551296 22.937058745743453 78-79 21.0134459616652 27.99275435174713 28.404680240621406 22.58911944596626 80-81 21.359958947475132 27.761163768011432 28.165439166909657 22.71343811760378 82-83 21.121197261438784 27.63798886231485 28.622977108842214 22.61783676740415 84-85 20.79704137732389 27.901736537434907 28.35887409131631 22.9423479939249 86-87 20.702855423396066 28.001931822890008 28.333200108978918 22.962012644735008 88-89 20.725341746060522 27.87181669251583 27.902890238416557 23.499951323007096 90-91 20.88088690271571 27.30166682038082 28.131262104989563 23.68618417191391 92-93 21.25980170221554 27.708242146201243 28.152775495922132 22.879180655661084 94-95 20.863783042140682 27.444536276070114 28.214040707189913 23.47763997459929 96-97 20.220843214987593 27.50350221294709 28.70733957649038 23.568314995574944 98-99 20.60507717516106 27.753794482869043 27.73381169398658 23.90731664798332 100-101 20.447515074391102 26.997201358581652 28.44808864015792 24.10719492686932 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 429.0 1 306.0 2 264.5 3 341.0 4 288.5 5 306.0 6 503.0 7 997.0 8 1594.0 9 1801.5 10 1627.0 11 1544.0 12 1706.0 13 1890.5 14 2075.5 15 2247.0 16 2450.5 17 2615.0 18 2830.5 19 3175.5 20 3671.5 21 4342.5 22 5282.0 23 6721.5 24 8368.0 25 10498.5 26 13850.0 27 18051.0 28 21952.0 29 26487.5 30 32651.0 31 38648.5 32 44659.5 33 51424.0 34 58623.0 35 65346.0 36 72520.5 37 79003.0 38 81088.5 39 81721.5 40 83143.5 41 85838.0 42 88456.0 43 90718.0 44 92672.0 45 93269.0 46 92956.5 47 103483.0 48 127574.0 49 123805.0 50 96063.5 51 79021.0 52 68361.5 53 60837.0 54 55118.0 55 49004.5 56 42545.0 57 36284.0 58 30532.5 59 25774.5 60 21757.0 61 17741.0 62 14233.0 63 11412.0 64 9136.5 65 7517.0 66 6245.5 67 5218.5 68 4315.0 69 3510.0 70 2826.0 71 2250.0 72 1781.0 73 1417.0 74 1130.0 75 842.0 76 571.0 77 370.5 78 241.5 79 166.0 80 101.5 81 54.5 82 30.5 83 18.0 84 11.5 85 6.5 86 2.5 87 2.0 88 2.0 89 2.5 90 2.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.29848762091570485 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0015258820957175388 16-17 7.733922950897114E-4 18-19 4.180498892376818E-5 20-21 0.0 22-23 0.0 24-25 0.0 26-27 2.090249446188409E-5 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0013168571510986978 36-37 0.007169555600426243 38-39 0.0026337143021973957 40-41 0.0030935691803588455 42-43 2.0902494461884092E-4 44-45 0.0010242222286323205 46-47 1.6721995569507272E-4 48-49 3.553424058520296E-4 50-51 2.090249446188409E-5 52-53 6.270748338565227E-5 54-55 0.0 56-57 6.479773283184069E-4 58-59 0.0019439319849552208 60-61 5.016598670852182E-4 62-63 9.824172397085525E-4 64-65 2.090249446188409E-5 66-67 3.7624490031391367E-4 68-69 0.0013586621400224661 70-71 0.0 72-73 0.0 74-75 6.270748338565227E-5 76-77 0.2581667090987304 78-79 1.0451247230942046E-4 80-81 6.270748338565227E-5 82-83 0.0 84-85 0.0 86-87 0.1089438011353399 88-89 0.376370315280685 90-91 0.2792155210218477 92-93 0.0 94-95 0.0 96-97 0.0 98-99 2.090249446188409E-5 100-101 2.29927439080725E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2392059.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.620519042302895 #Duplication Level Percentage of deduplicated Percentage of total 1 67.76151000044291 30.913152573104036 2 15.370386231116075 14.024099954883622 3 6.487585367500939 8.879010353899266 4 3.3747643528684375 6.158340056932783 5 2.009359012239953 4.583400054035785 6 1.2706103832347861 3.4779543110226205 7 0.8345309179595728 2.665021354391564 8 0.5737119620475772 2.093842999151076 9 0.42010472780584496 1.7248856161165222 >10 1.74357014604702 13.382561656120902 >50 0.09934187364860991 3.1301025723414004 >100 0.05049080428865316 3.9785925292876363 >500 0.0016503630543217638 0.493821493579056 >1k 0.0019254235633745407 2.499934711733807 >5k 3.6674734540467443E-4 1.2341772818514851 >10k+ 9.168683635116861E-5 0.7611024815484858 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 18196 0.7606835784568858 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 9023 0.3772064150591603 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 7358 0.3076011085010863 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6637 0.27745971148704945 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 6488 0.271230768137408 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4945 0.20672567022803368 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 4783 0.19995326202238323 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 4323 0.18072296711744987 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 4311 0.18022130725036464 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 4100 0.17140045458744957 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 4014 0.16780522554000551 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3578 0.14957825036924255 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 3475 0.14527233651009444 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 3428 0.14330750203067735 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2783 0.11634328417484685 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 4.180498892376819E-5 0.0 0.0 0.0 0.0 12-13 4.180498892376819E-5 0.0 0.0 0.0 0.0 14-15 4.180498892376819E-5 0.0 0.0 0.0 0.0 16-17 4.180498892376819E-5 0.0 0.0 4.180498892376819E-5 0.0 18-19 4.180498892376819E-5 0.0 0.0 8.360997784753637E-5 0.0 20-21 4.180498892376819E-5 0.0 0.0 2.508299335426091E-4 0.0 22-23 8.360997784753637E-5 0.0 0.0 5.016598670852182E-4 0.0 24-25 8.360997784753637E-5 0.0 0.0 9.406122507847842E-4 0.0 26-27 8.360997784753637E-5 0.0 0.0 0.0014004671289462342 0.0 28-29 8.360997784753637E-5 0.0 0.0 0.004368621342533775 0.0 30-31 8.360997784753637E-5 0.0 0.0 0.016889215525202346 0.0 32-33 8.360997784753637E-5 0.0 0.0 0.043916140864418474 0.0 34-35 8.360997784753637E-5 0.0 0.0 0.08611827718296246 0.0 36-37 8.360997784753637E-5 0.0 0.0 0.15574448623549836 0.0 38-39 8.360997784753637E-5 0.0 0.0 0.2616574256738651 0.0 40-41 8.360997784753637E-5 0.0 0.0 0.37906673706626803 4.180498892376819E-5 42-43 8.360997784753637E-5 0.0 0.0 0.5006565473510478 4.180498892376819E-5 44-45 1.2541496677130455E-4 0.0 0.0 0.646890398606389 4.180498892376819E-5 46-47 1.2541496677130455E-4 0.0 0.0 0.8246034065213275 4.180498892376819E-5 48-49 1.2541496677130455E-4 0.0 0.0 1.0101339473650106 4.180498892376819E-5 50-51 1.2541496677130455E-4 0.0 0.0 1.2048824882663847 4.180498892376819E-5 52-53 1.2541496677130455E-4 0.0 0.0 1.4044595053884539 4.180498892376819E-5 54-55 1.2541496677130455E-4 0.0 0.0 1.6309380328829681 4.180498892376819E-5 56-57 1.2541496677130455E-4 0.0 0.0 1.9140414178747265 4.180498892376819E-5 58-59 1.2541496677130455E-4 0.0 0.0 2.2144311657864626 4.180498892376819E-5 60-61 1.2541496677130455E-4 0.0 0.0 2.5383780249567423 4.180498892376819E-5 62-63 1.6721995569507275E-4 0.0 0.0 2.8659828206578517 4.180498892376819E-5 64-65 1.6721995569507275E-4 0.0 0.0 3.2227674986277512 4.180498892376819E-5 66-67 2.0902494461884092E-4 0.0 0.0 3.631787510257899 4.180498892376819E-5 68-69 2.0902494461884092E-4 0.0 0.0 4.093021953053833 4.180498892376819E-5 70-71 2.508299335426091E-4 0.0 0.0 4.58734504458293 4.180498892376819E-5 72-73 3.344399113901455E-4 0.0 0.0 5.076902367374718 4.180498892376819E-5 74-75 3.7624490031391367E-4 0.0 0.0 5.608933558913053 4.180498892376819E-5 76-77 3.7624490031391367E-4 0.0 0.0 6.170186437709104 4.180498892376819E-5 78-79 3.7624490031391367E-4 0.0 0.0 6.836620668637353 4.180498892376819E-5 80-81 5.016598670852182E-4 0.0 0.0 7.5518831266285655 4.180498892376819E-5 82-83 5.016598670852182E-4 0.0 0.0 8.258241121979015 4.180498892376819E-5 84-85 5.016598670852182E-4 0.0 0.0 8.985689734241504 4.180498892376819E-5 86-87 5.016598670852182E-4 0.0 0.0 9.746645045126396 4.180498892376819E-5 88-89 5.016598670852182E-4 0.0 0.0 10.57488548568409 4.180498892376819E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 7945 0.0 25.76755 1 TACATGG 7805 0.0 25.354874 2 ACATGGG 8155 0.0 23.451973 3 GAGTACT 6680 0.0 21.597551 12-13 CATGGGG 3540 0.0 20.376925 4 GTAAGCG 290 0.0 19.637291 94-95 AGAGTAC 11435 0.0 19.505835 10-11 AGTACTT 7555 0.0 19.221813 12-13 GTACTTT 7725 0.0 18.798811 14-15 GTGTAGC 1455 0.0 18.324139 1 AGTACAT 6090 0.0 18.234627 2 CATGGGA 4535 0.0 18.208336 4 GAGTACA 6745 0.0 18.140518 1 ATAAGCG 180 0.002635673 15.818928 3 TACTTTT 9310 0.0 15.776779 14-15 TGTATCG 275 1.1385942E-5 15.531311 9 GTATAGG 590 0.0 15.3320465 1 ACTTTTT 9730 0.0 15.120154 16-17 ACCGCTC 190 0.0037783084 14.986353 8 TAATACA 1210 0.0 14.511579 4 >>END_MODULE