FastQCFastQC Report
Fri 27 May 2016
SRR939300_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939300_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2441922
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT177910.7285654496744777No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT94110.3853931452355972No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT77370.3168405870457779No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70080.286987053640534No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC66170.2709750761899848No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49050.20086636673898678No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT48550.19881879929006743No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT42830.17539462767443023No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC41820.17125854142761315No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC38500.15766269356678878No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG38040.155778931513783No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC35330.1446811159406402No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34170.13993075945914735No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA32060.13129002482470775No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA25650.10504021012956188No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG81200.025.4485511
TACATGG79150.025.0446822
ACATGGG81450.023.5223333
GAGTACT64400.021.12079212-13
AGTACAT60400.020.5709572
CATGGGG35900.020.3430654
GGACCGA1708.1027276E-519.5131656
GAGTACA69150.019.3038521
AGTACTT70750.018.52178612-13
GTACTTT73100.018.34795614-15
AGAGTAC108500.018.1702110-11
CATGGGA44650.018.1620464
GTGTAGC14400.018.1438751
TGGACCG1750.002194288216.2593565
GTATAGG6300.015.8346551
GTAAGAC7650.015.4977513
CTGGTCG5850.015.3913369
TGTACCG2551.0463514E-414.8778425
ACTTTTT94950.014.75083916-17
GTAGCCC14250.014.6429293
Produced by FastQC (version 0.11.5)