##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939300_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2441922 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.891935942261874 33.0 31.0 34.0 30.0 34.0 2 31.964511560975332 33.0 31.0 34.0 30.0 34.0 3 32.03081998524113 34.0 31.0 34.0 30.0 34.0 4 35.52647095197963 37.0 35.0 37.0 33.0 37.0 5 35.57957543279433 37.0 35.0 37.0 33.0 37.0 6 35.67133389190973 37.0 35.0 37.0 33.0 37.0 7 35.596269659718864 37.0 35.0 37.0 33.0 37.0 8 35.58795162171437 37.0 35.0 37.0 33.0 37.0 9 37.22154065527072 39.0 37.0 39.0 34.0 39.0 10-11 37.131857815278295 39.0 37.0 39.0 33.0 39.0 12-13 37.048778789822116 39.0 37.0 39.0 33.0 39.0 14-15 38.28601282104834 40.0 38.0 41.0 33.0 41.0 16-17 38.240574842275876 40.0 38.0 41.0 33.0 41.0 18-19 38.200734503395275 40.0 38.0 41.0 33.0 41.0 20-21 38.102176891808995 40.0 38.0 41.0 33.0 41.0 22-23 38.03170842475722 40.0 38.0 41.0 33.0 41.0 24-25 38.05016745006597 40.0 38.0 41.0 33.0 41.0 26-27 37.978119284727356 40.0 38.0 41.0 33.0 41.0 28-29 37.87168816202974 40.0 38.0 41.0 33.0 41.0 30-31 37.72823824839614 40.0 37.5 41.0 32.5 41.0 32-33 37.54890901511186 40.0 37.0 41.0 32.0 41.0 34-35 37.247965536982754 40.0 37.0 41.0 31.0 41.0 36-37 37.01148890095589 40.0 36.5 41.0 30.5 41.0 38-39 36.720699719319455 39.5 36.0 41.0 30.0 41.0 40-41 36.64067525498358 39.0 36.0 41.0 30.0 41.0 42-43 36.49535611702585 39.0 36.0 41.0 30.0 41.0 44-45 36.48323206064731 39.5 36.0 41.0 30.0 41.0 46-47 36.34178589651921 40.0 36.0 41.0 30.0 41.0 48-49 36.16998802582556 40.0 36.0 41.0 29.0 41.0 50-51 35.04517507111202 38.0 34.5 40.0 26.5 40.5 52-53 35.20273497679287 38.5 35.0 39.5 27.5 40.5 54-55 35.75924968119375 39.0 35.0 40.5 28.0 41.0 56-57 35.684239914297024 39.0 35.0 41.0 27.5 41.0 58-59 35.5152961478704 39.0 35.0 41.0 27.0 41.0 60-61 35.241605587729666 39.0 35.0 41.0 26.5 41.0 62-63 34.93693307976258 38.0 34.5 40.0 26.0 41.0 64-65 34.593113129739606 38.0 34.0 40.0 26.0 41.0 66-67 34.233338124641165 37.5 34.0 40.0 25.5 41.0 68-69 33.82594816705857 37.0 34.0 39.5 24.0 41.0 70-71 33.397173210282716 36.0 33.0 39.0 23.0 41.0 72-73 32.948570838872 36.0 33.0 39.0 22.0 40.0 74-75 32.50033743911558 35.0 32.0 38.0 21.5 40.0 76-77 32.04679674453156 35.0 32.0 37.0 20.0 39.0 78-79 31.59428454307713 35.0 31.5 37.0 18.5 39.0 80-81 31.08781341091157 35.0 31.0 36.0 16.0 38.5 82-83 30.61311458760763 35.0 31.0 36.0 10.0 37.0 84-85 30.162325209404724 34.0 30.5 35.0 7.0 37.0 86-87 29.827821077004096 34.0 30.0 35.0 2.0 36.5 88-89 29.38967849914944 34.0 30.0 35.0 2.0 36.0 90-91 28.956806564665044 34.0 29.0 35.0 2.0 36.0 92-93 28.522861704837418 34.0 29.0 35.0 2.0 35.5 94-95 28.02499097022755 34.0 29.0 35.0 2.0 35.0 96-97 27.44704171550115 34.0 27.0 35.0 2.0 35.0 98-99 26.733422689176805 34.0 25.5 35.0 2.0 35.0 100-101 24.253041456688624 31.0 13.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.33030014851919987 1101 2 0.16207176194974338 1101 3 0.34548863916384676 1101 4 0.2961316722052487 1101 5 0.31951716058524937 1101 6 0.3586321130365704 1101 7 0.3325717260321781 1101 8 0.3993759504692491 1101 9 0.32352451019209383 1101 10-11 0.4486440252623396 1101 12-13 0.41358243862055133 1101 14-15 0.47441006869716773 1101 16-17 0.4302745409931319 1101 18-19 0.5205732799008587 1101 20-21 0.43921089142752123 1101 22-23 0.48808441153336446 1101 24-25 0.5337249583888806 1101 26-27 0.47266763182640403 1101 28-29 0.39422099204439576 1101 30-31 0.5375640668705728 1101 32-33 0.4926378112285761 1101 34-35 0.5981022329035568 1101 36-37 0.5155418386802353 1101 38-39 0.6002360854052924 1101 40-41 0.501255964457691 1101 42-43 0.4353307451058086 1101 44-45 0.5132784307263307 1101 46-47 0.5132484210814781 1101 48-49 0.5543737693882136 1101 50-51 0.5713505451194152 1101 52-53 0.6241428467867678 1101 54-55 0.6102843901986361 1101 56-57 0.7080348752451684 1101 58-59 0.5906745282835217 1101 60-61 0.6619061978400822 1101 62-63 0.6419546833548253 1101 64-65 0.5291757682667111 1101 66-67 0.6140611224136379 1101 68-69 0.5700795745948284 1101 70-71 0.5880255873195281 1101 72-73 0.6575015328126668 1101 74-75 0.516294035564762 1101 76-77 0.37179967647209367 1101 78-79 0.45511153667533577 1101 80-81 0.3522732028800668 1101 82-83 0.31708489906229786 1101 84-85 0.2506696322243691 1101 86-87 0.12040535964587917 1101 88-89 0.23093213803988277 1101 90-91 0.3443057077871643 1101 92-93 0.3568969168910776 1101 94-95 0.3229341490575486 1101 96-97 0.20129016189246585 1101 98-99 0.23908361988609528 1101 100-101 -0.04351830132578982 1102 1 0.29040813899304396 1102 2 0.29701920384841074 1102 3 0.40665436142712963 1102 4 0.298687309882844 1102 5 0.368907815097856 1102 6 0.3025070411797941 1102 7 0.3466889141737539 1102 8 0.3516659233689765 1102 9 0.3934292486334172 1102 10-11 0.40600113292433093 1102 12-13 0.4097836786632598 1102 14-15 0.5613130415147651 1102 16-17 0.6120730135233075 1102 18-19 0.6520434696027593 1102 20-21 0.6457137246376732 1102 22-23 0.5735157575134906 1102 24-25 0.55800372163106 1102 26-27 0.5426937055719918 1102 28-29 0.6128039763590891 1102 30-31 0.5931824457816361 1102 32-33 0.5779597295992858 1102 34-35 0.5793198973529314 1102 36-37 0.6524848459891004 1102 38-39 0.7243292528521224 1102 40-41 0.85640029393128 1102 42-43 0.9386423161003066 1102 44-45 0.8298231461734815 1102 46-47 0.8091293851941472 1102 48-49 0.7412020111554725 1102 50-51 0.6573105516891786 1102 52-53 0.7785293951977081 1102 54-55 0.7971951646094126 1102 56-57 0.7019707789981524 1102 58-59 0.6845445291047341 1102 60-61 0.5599603162600602 1102 62-63 0.5404666321106788 1102 64-65 0.6763885464550938 1102 66-67 0.7158957286384933 1102 68-69 0.6331121359810439 1102 70-71 0.552995202167196 1102 72-73 0.6329825626229635 1102 74-75 0.4112347023956673 1102 76-77 0.5763712047631344 1102 78-79 0.5722384973388834 1102 80-81 0.4653141817747013 1102 82-83 0.31592430942609795 1102 84-85 0.37620794215292364 1102 86-87 0.4673300948023211 1102 88-89 0.38377068612233245 1102 90-91 0.39787391013584994 1102 92-93 0.6473015732103669 1102 94-95 0.613537540697525 1102 96-97 0.3749484931727487 1102 98-99 0.46185544381645727 1102 100-101 0.135385574834185 1103 1 0.2408189119027071 1103 2 0.2584144537166857 1103 3 0.3095873245852374 1103 4 0.2885191098260975 1103 5 0.27706369399381714 1103 6 0.27076444806181144 1103 7 0.24917969356823733 1103 8 0.23572076064048986 1103 9 0.3854338690687129 1103 10-11 0.43863245055898403 1103 12-13 0.3436939246811761 1103 14-15 0.48170547453872814 1103 16-17 0.47348062508250166 1103 18-19 0.4921738753094047 1103 20-21 0.4739099310171184 1103 22-23 0.49614059032668933 1103 24-25 0.5270698060148007 1103 26-27 0.49804297400307007 1103 28-29 0.38595418103626145 1103 30-31 0.4524117652806865 1103 32-33 0.46144430788324087 1103 34-35 0.4223431413848644 1103 36-37 0.46623084363834977 1103 38-39 0.5280704882106093 1103 40-41 0.5234135270200895 1103 42-43 0.3871720854838472 1103 44-45 0.4877210507655434 1103 46-47 0.48236804688911405 1103 48-49 0.4171486937639912 1103 50-51 0.37524047508722447 1103 52-53 0.4368184155843622 1103 54-55 0.4853112226485692 1103 56-57 0.5084103080379876 1103 58-59 0.405354929137161 1103 60-61 0.6033318154697227 1103 62-63 0.5338067204663659 1103 64-65 0.525428387576973 1103 66-67 0.4497866215173474 1103 68-69 0.4616158647533908 1103 70-71 0.35913693604633323 1103 72-73 0.443830757005486 1103 74-75 0.45218300620955887 1103 76-77 0.500385005273813 1103 78-79 0.5344950125579508 1103 80-81 0.3487697810127379 1103 82-83 0.3474665823560201 1103 84-85 0.23180636958371892 1103 86-87 0.2671062671364517 1103 88-89 0.40384789602789795 1103 90-91 0.43790957665390806 1103 92-93 0.4765129581250527 1103 94-95 0.44128659837190654 1103 96-97 0.37236520374706217 1103 98-99 0.07515003709656298 1103 100-101 0.021602796088185272 1104 1 0.24046306655235128 1104 2 0.26696586839227265 1104 3 0.337247419857313 1104 4 0.3486220336529442 1104 5 0.3040630451567665 1104 6 0.2243833347636155 1104 7 0.3144941999562718 1104 8 0.39403895314130466 1104 9 0.38921972785647085 1104 10-11 0.3836579550864059 1104 12-13 0.4086727886775918 1104 14-15 0.3645446129205965 1104 16-17 0.29201569780924785 1104 18-19 0.30333506329803583 1104 20-21 0.39629006598458716 1104 22-23 0.4641155823821421 1104 24-25 0.4222685679931075 1104 26-27 0.3700902188660464 1104 28-29 0.31457439508221796 1104 30-31 0.37609201432168504 1104 32-33 0.40571088387407883 1104 34-35 0.3816423778496656 1104 36-37 0.5696692115359809 1104 38-39 0.5527208306263631 1104 40-41 0.5259549030699517 1104 42-43 0.6220963839238962 1104 44-45 0.6214255980123653 1104 46-47 0.6712210107314789 1104 48-49 0.6184671759381501 1104 50-51 0.6486009513070741 1104 52-53 0.7709239183628043 1104 54-55 0.7392167376073431 1104 56-57 0.6829495244208559 1104 58-59 0.6779906364124955 1104 60-61 0.6369082544864568 1104 62-63 0.6926852278756783 1104 64-65 0.8711895335590683 1104 66-67 0.6474758910330181 1104 68-69 0.6931714418240631 1104 70-71 0.6109390009253559 1104 72-73 0.526463298174626 1104 74-75 0.594513240844968 1104 76-77 0.7254394263839004 1104 78-79 0.6821708329542311 1104 80-81 0.6616205024242809 1104 82-83 0.6077006985361102 1104 84-85 0.6057828506069924 1104 86-87 0.5583773006612631 1104 88-89 0.5870074318634586 1104 90-91 0.6417005301008274 1104 92-93 0.7422072410976455 1104 94-95 0.6698530159403226 1104 96-97 0.7839339254287587 1104 98-99 0.7804627515699991 1104 100-101 0.7229253774133895 1105 1 0.2996433050246239 1105 2 0.3563873506716426 1105 3 0.41978874201659977 1105 4 0.3479104220999645 1105 5 0.3168967722484908 1105 6 0.21549755366132217 1105 7 0.32972530891673557 1105 8 0.3466231517693217 1105 9 0.37057288344172434 1105 10-11 0.4407306997646714 1105 12-13 0.3849957869142884 1105 14-15 0.465790644749859 1105 16-17 0.4502664966868437 1105 18-19 0.5519472335035331 1105 20-21 0.5379530122961214 1105 22-23 0.5764178105502751 1105 24-25 0.5232383821800894 1105 26-27 0.4918808507621293 1105 28-29 0.5555176399410158 1105 30-31 0.6360703064239885 1105 32-33 0.6219052152513527 1105 34-35 0.5662930290116961 1105 36-37 0.70251323077796 1105 38-39 0.7508649476962006 1105 40-41 0.6924336168957481 1105 42-43 0.5539487162869463 1105 44-45 0.5628813155490207 1105 46-47 0.7584724523066697 1105 48-49 0.7181051529238829 1105 50-51 0.7188242774843445 1105 52-53 0.6245201409788663 1105 54-55 0.6598915251351158 1105 56-57 0.7069437551238238 1105 58-59 0.789417769636664 1105 60-61 0.7069946890728716 1105 62-63 0.671556919069225 1105 64-65 0.4962524550016454 1105 66-67 0.7545511450783451 1105 68-69 0.6196248896943999 1105 70-71 0.5243658375198663 1105 72-73 0.6379419296032793 1105 74-75 0.7257889056774189 1105 76-77 0.6399436054163772 1105 78-79 0.6322093348845677 1105 80-81 0.6962613782907603 1105 82-83 0.6010458196523949 1105 84-85 0.6156075844268152 1105 86-87 0.529024822230582 1105 88-89 0.6169960535692596 1105 90-91 0.6092939272444937 1105 92-93 0.647456911883669 1105 94-95 0.577773289978186 1105 96-97 0.42773545172317995 1105 98-99 0.43054293895604445 1105 100-101 0.22539354590502825 1106 1 0.16345270003635193 1106 2 0.23533656820717752 1106 3 0.16424551765692996 1106 4 0.28110014589668 1106 5 0.22079215409637243 1106 6 0.1601229112987994 1106 7 0.2752366880226589 1106 8 0.24150072052498217 1106 9 0.20077535714640504 1106 10-11 0.28428190171638335 1106 12-13 0.35273632304940605 1106 14-15 0.4141388846386036 1106 16-17 0.3909357944061611 1106 18-19 0.3683807761690545 1106 20-21 0.42721043578933404 1106 22-23 0.44639635140725886 1106 24-25 0.41897469231658846 1106 26-27 0.40035320521079143 1106 28-29 0.4843143752032333 1106 30-31 0.37035080439918033 1106 32-33 0.3985541936880992 1106 34-35 0.43186400213982523 1106 36-37 0.4901159246553064 1106 38-39 0.5848546131045822 1106 40-41 0.7016741428343707 1106 42-43 0.5068631061843334 1106 44-45 0.6385245263491441 1106 46-47 0.4545895388004695 1106 48-49 0.563353355210829 1106 50-51 0.6020063807451095 1106 52-53 0.5575950200788924 1106 54-55 0.6278037916578185 1106 56-57 0.6614572053697643 1106 58-59 0.6732915349220079 1106 60-61 0.7394358114883772 1106 62-63 0.7971929923251153 1106 64-65 0.697122358857662 1106 66-67 0.6632068574929022 1106 68-69 0.6315487923819418 1106 70-71 0.6739828072012202 1106 72-73 0.5784457579387308 1106 74-75 0.6129094486030411 1106 76-77 0.6436926944403325 1106 78-79 0.5335682946849936 1106 80-81 0.4977844300542351 1106 82-83 0.5640507114640414 1106 84-85 0.4187219150056656 1106 86-87 0.44447801693456057 1106 88-89 0.4865236232730119 1106 90-91 0.416945428329079 1106 92-93 0.5578716085702418 1106 94-95 0.47146851290390757 1106 96-97 0.3569914763292843 1106 98-99 0.4533290569870587 1106 100-101 0.2773226684808101 1107 1 0.23830624759585106 1107 2 0.3093529439338276 1107 3 0.20095423940150425 1107 4 0.2583258089676477 1107 5 0.2676204447500865 1107 6 0.16636152562152517 1107 7 0.25138079946966485 1107 8 0.1693169300803845 1107 9 0.3144649411288398 1107 10-11 0.3671016515332326 1107 12-13 0.40072098799821987 1107 14-15 0.4455656345593155 1107 16-17 0.48361740034453504 1107 18-19 0.5246525300054472 1107 20-21 0.4753728155467627 1107 22-23 0.4983815293255702 1107 24-25 0.3857965831319703 1107 26-27 0.5191755542602223 1107 28-29 0.4895878384701291 1107 30-31 0.5646711584112936 1107 32-33 0.5167150682203925 1107 34-35 0.6044419229783173 1107 36-37 0.6561013804613367 1107 38-39 0.7370755920833574 1107 40-41 0.6570272005816804 1107 42-43 0.831797441176569 1107 44-45 0.8860395891264972 1107 46-47 0.8129403980068375 1107 48-49 0.8806033229309449 1107 50-51 0.8611383430253596 1107 52-53 0.9712315001397442 1107 54-55 1.0676001371536472 1107 56-57 0.9275031157888733 1107 58-59 0.9180840349009571 1107 60-61 0.8391221527457162 1107 62-63 0.9636987858629666 1107 64-65 1.0236155141183048 1107 66-67 0.7649736725067058 1107 68-69 0.9828558821677476 1107 70-71 0.8521783931372937 1107 72-73 0.8129154485290044 1107 74-75 0.9489459592596106 1107 76-77 0.9577371291072438 1107 78-79 0.908626538827594 1107 80-81 0.9148832787850729 1107 82-83 1.0791312953931502 1107 84-85 1.083264073940601 1107 86-87 1.0313638043813356 1107 88-89 0.9858573243163526 1107 90-91 1.0884779369814908 1107 92-93 0.9556702431310491 1107 94-95 0.8494901032356559 1107 96-97 1.0459025810312 1107 98-99 1.1729384212417173 1107 100-101 0.9044368255968429 1108 1 0.06489816116599556 1108 2 0.056335537920841006 1108 3 0.08826190022978864 1108 4 0.07083808593605312 1108 5 0.12014950024197901 1108 6 -0.01910049762287258 1108 7 0.053122764875901396 1108 8 0.11780413475108986 1108 9 0.1174721163348238 1108 10-11 0.16253291415122817 1108 12-13 0.10902055966843704 1108 14-15 0.06738658179563117 1108 16-17 0.16272711551886943 1108 18-19 0.23060038017186457 1108 20-21 0.2515415147337521 1108 22-23 0.31882063556226825 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2316 88-89 -1.1055760066114573 2316 90-91 -1.1885060181795595 2316 92-93 -1.2516484104976797 2316 94-95 -1.3439787512542516 2316 96-97 -1.29274049346785 2316 98-99 -1.392827677721506 2316 100-101 -0.9372968950257068 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6233.0 3 1514.0 4 1500.0 5 1710.0 6 2016.0 7 2409.0 8 3108.0 9 3920.0 10 4831.0 11 6427.0 12 8640.0 13 12623.0 14 11387.0 15 11990.0 16 14627.0 17 18643.0 18 15924.0 19 12406.0 20 12851.0 21 13808.0 22 15556.0 23 17270.0 24 19701.0 25 22596.0 26 26358.0 27 31070.0 28 36920.0 29 44305.0 30 52979.0 31 64309.0 32 80070.0 33 102206.0 34 135584.0 35 192606.0 36 295028.0 37 486572.0 38 547419.0 39 104779.0 40 27.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.90698791855683 20.036025025318548 14.191198756126349 22.865788299998275 2 14.508842185357917 21.628342877383552 40.36612583715423 23.496689100104295 3 19.48943578238187 25.43021941887373 30.63237703506536 24.447967763679035 4 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21.5810463461862 24-25 21.60604609249938 27.715506402464708 29.573380109106584 21.105067395929336 26-27 21.40843148008332 27.401449514164366 30.26873231862423 20.92138668712808 28-29 20.70309009786635 28.73802491784358 29.727037393159765 20.831847591130305 30-31 21.239205721428718 27.71372364187791 29.482653333060107 21.56441730363327 32-33 21.065039312672162 27.996552623614484 29.614999939552067 21.323408124161286 34-35 21.981915487106566 27.685881034003597 29.397288267234046 20.934915211655795 36-37 21.62591838533929 27.3234781740205 29.850290165685955 21.20031327495426 38-39 21.07533252825016 28.140183909047238 29.814813064700168 20.969670498002426 40-41 21.25925102752677 28.160365043404777 29.508798143395882 21.07158578567257 42-43 20.990338526540544 28.204340071209273 28.82989186702535 21.975429535224837 44-45 21.418762204836376 27.72745664104409 28.970302754946097 21.883478399173438 46-47 21.86746576947073 28.19675372020935 28.727566334109035 21.20821417621088 48-49 21.652961285200387 27.94348166631625 28.874321333391702 21.529235715091662 50-51 20.710295447114405 28.297657167248225 29.45751361451327 21.5345337711241 52-53 21.445642146261747 28.670030352085423 28.35785743747345 21.52647006417938 54-55 20.932319870102678 27.92527369651131 28.883619881013757 22.258786552372257 56-57 21.559249205947793 27.985212068073295 28.63109529181123 21.824443434167677 58-59 22.11730632952993 27.72773675399768 28.588380285306414 21.566576631165976 60-61 21.506093173038725 27.93569189927761 29.305128818434632 21.253086109249036 62-63 21.012248447010993 28.010674192624467 29.26796143713687 21.70911592322767 64-65 20.813668048066464 28.54345079205096 28.797170994057048 21.845710165825523 66-67 21.204414300333642 27.986181520867177 28.208980654910242 22.60042352388894 68-69 20.883099242390603 27.953291169007578 28.73180594543305 22.43180364316877 70-71 21.47326317970577 27.653948238265798 28.670731827645067 22.202056754383367 72-73 21.376453266118492 27.148371667825828 29.335649187190377 22.139525878865303 74-75 20.745249239576196 28.435092106904726 28.579171324577178 22.240487328941896 76-77 20.9549684845974 27.538473615508803 28.64765714135406 22.85890075853974 78-79 20.960315148158177 27.64636560161305 28.783444120331243 22.60987512989753 80-81 21.358919120145654 27.398559654126338 28.613756465921934 22.628764759806074 82-83 21.144557790280842 27.232418233396714 29.102939599130256 22.520084377192187 84-85 20.89125334080781 27.50965921397157 28.84419454628922 22.754892898931395 86-87 20.804341867177133 27.517793510212957 28.864876993717665 22.812987628892248 88-89 20.801923270974584 27.279193452691057 28.50240563006422 23.41647764627014 90-91 20.943152383072608 26.737930979193607 28.757353731684148 23.561562906049634 92-93 21.36704280364392 27.012591822006364 28.846637285269033 22.77372808908068 94-95 21.010314118325617 26.665484065511446 28.99371467355892 23.330487142604024 96-97 20.469136101928804 26.644670237540467 29.469349680115787 23.416843980414942 98-99 20.889331113804563 26.861936691634654 28.515011519337875 23.733720675222912 100-101 20.764675965169147 25.883167655595795 29.190660399167754 24.1614959800673 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1888.0 1 1103.0 2 371.0 3 423.0 4 379.5 5 408.5 6 622.5 7 1173.0 8 1917.0 9 2217.0 10 2134.5 11 2174.5 12 2311.5 13 2501.0 14 2754.0 15 2968.0 16 3137.0 17 3262.5 18 3401.0 19 3633.0 20 4001.0 21 4615.5 22 5479.5 23 6908.5 24 8510.5 25 10455.0 26 14057.0 27 18318.5 28 22235.0 29 27110.5 30 33263.0 31 39114.5 32 45127.0 33 52115.5 34 59798.5 35 66706.0 36 73662.0 37 80602.5 38 83113.0 39 83613.5 40 84940.0 41 87263.5 42 89587.0 43 91576.0 44 93691.5 45 94439.5 46 94339.0 47 103371.0 48 126321.0 49 124200.5 50 97239.0 51 80188.0 52 69849.0 53 62845.5 54 56811.5 55 50064.0 56 43730.0 57 37663.5 58 31819.0 59 26799.0 60 22535.5 61 18377.0 62 14822.5 63 11966.5 64 9521.0 65 7830.5 66 6467.0 67 5447.5 68 4674.0 69 3770.5 70 3006.0 71 2419.5 72 1926.5 73 1513.0 74 1205.0 75 950.0 76 694.0 77 460.5 78 303.0 79 216.0 80 137.5 81 83.0 82 57.5 83 44.0 84 28.0 85 16.5 86 11.5 87 12.0 88 11.0 89 7.0 90 5.5 91 6.5 92 4.0 93 2.5 94 3.5 95 2.5 96 1.5 97 1.5 98 0.5 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2845299727018308 2 0.1108143503355144 3 0.12015125790258657 4 0.12309975502903042 5 0.1292834087247668 6 0.05778235340850363 7 0.06736496906944611 8 0.07219722824889574 9 0.06904397437755998 10-11 0.06437552059402389 12-13 0.06888016898164642 14-15 0.06441647194300228 16-17 0.06548120701644033 18-19 0.0698834770316169 20-21 0.07428574704679347 22-23 0.08241458981900324 24-25 0.08999058938000476 26-27 0.09042057854427782 28-29 0.08452358429139013 30-31 0.08075606018537856 32-33 0.07393766058047718 34-35 0.2422272292071573 36-37 0.3651017518168066 38-39 0.3365791372533603 40-41 0.31944099770590545 42-43 0.3436022936031536 44-45 0.3569524333701076 46-47 0.3628289519485062 48-49 0.3734148756594191 50-51 0.36645314633309334 52-53 0.3675588327555098 54-55 0.3528163471232906 56-57 0.3294740782056102 58-59 0.3152025330866424 60-61 0.2708726978175388 62-63 0.343540866579686 64-65 0.3736401080788002 66-67 0.3782880861878471 68-69 0.3904301611599388 70-71 0.39092157734767946 72-73 0.373722010776757 74-75 0.3780833294429552 76-77 0.36143660608324096 78-79 0.3387905100981931 80-81 0.3358420129717493 82-83 0.3506664013019253 84-85 0.35844715760781876 86-87 0.3518130390733201 88-89 0.36788644354733685 90-91 0.39184298269969314 92-93 0.38619169654067576 94-95 0.3862121722151649 96-97 0.3913925178609308 98-99 0.379762334751069 100-101 0.3576895576517186 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2441922.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.60586559726224 #Duplication Level Percentage of deduplicated Percentage of total 1 68.89780705751006 32.1104193566842 2 14.717333700952926 13.718281528333401 3 6.367215098130232 8.902487132769494 4 3.3028725394775553 6.157329346391166 5 1.91043761152634 4.451879927737564 6 1.2126014093166015 3.390860298339618 7 0.8098394298479676 2.6420287336000507 8 0.5664792484623493 2.1121004573979496 9 0.40175100076495557 1.6851557830695405 >10 1.6664922118594026 13.119874106130435 >50 0.09581295201049536 3.0637084024602745 >100 0.04774470628441594 3.8527969486518066 >500 0.0012337188779308246 0.3636782415260081 >1k 0.0019387010938905016 2.4348576565626114 >5k 3.5249110798009126E-4 1.2638588962705783 >10k+ 8.812277699502281E-5 0.7306831840753213 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 17791 0.7285654496744777 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 9411 0.3853931452355972 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 7737 0.3168405870457779 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7008 0.286987053640534 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 6617 0.2709750761899848 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4905 0.20086636673898678 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 4855 0.19881879929006743 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 4283 0.17539462767443023 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 4182 0.17125854142761315 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3850 0.15766269356678878 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 3804 0.155778931513783 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 3533 0.1446811159406402 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3417 0.13993075945914735 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 3206 0.13129002482470775 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2565 0.10504021012956188 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 4.09513489783867E-5 0.0 16-17 0.0 0.0 0.0 8.19026979567734E-5 0.0 18-19 0.0 0.0 0.0 8.19026979567734E-5 0.0 20-21 0.0 0.0 0.0 1.0237837244596674E-4 0.0 22-23 0.0 0.0 0.0 2.047567448919335E-4 0.0 24-25 0.0 0.0 0.0 5.733188856974138E-4 0.0 26-27 0.0 0.0 2.047567448919335E-5 0.001289967492819181 0.0 28-29 0.0 0.0 4.09513489783867E-5 0.004607026760068503 0.0 30-31 0.0 0.0 4.09513489783867E-5 0.016892431453584513 0.0 32-33 0.0 0.0 4.09513489783867E-5 0.044739348758887465 0.0 34-35 2.047567448919335E-5 0.0 4.09513489783867E-5 0.08767683816272592 0.0 36-37 4.09513489783867E-5 0.0 4.09513489783867E-5 0.1551032342556396 0.0 38-39 4.09513489783867E-5 0.0 4.09513489783867E-5 0.2568263851179522 0.0 40-41 4.09513489783867E-5 0.0 4.09513489783867E-5 0.3640984437668361 0.0 42-43 1.0237837244596674E-4 0.0 4.09513489783867E-5 0.4799702857011813 0.0 44-45 2.0475674489193348E-4 0.0 4.09513489783867E-5 0.6254704286213892 0.0 46-47 2.0475674489193348E-4 0.0 4.09513489783867E-5 0.8013359968090709 0.0 48-49 2.0475674489193348E-4 0.0 4.09513489783867E-5 0.9805800512874695 0.0 50-51 2.0475674489193348E-4 0.0 4.09513489783867E-5 1.1776174668969772 0.0 52-53 2.0475674489193348E-4 0.0 4.09513489783867E-5 1.372259228591249 0.0 54-55 2.0475674489193348E-4 0.0 4.09513489783867E-5 1.5971230858315706 0.0 56-57 2.0475674489193348E-4 0.0 4.09513489783867E-5 1.8741589616703564 0.0 58-59 2.0475674489193348E-4 0.0 4.09513489783867E-5 2.165650663698513 0.0 60-61 2.0475674489193348E-4 0.0 4.09513489783867E-5 2.4764099754210003 0.0 62-63 2.0475674489193348E-4 0.0 4.09513489783867E-5 2.7997618269543416 0.0 64-65 2.0475674489193348E-4 0.0 4.09513489783867E-5 3.146783558197191 0.0 66-67 2.457080938703202E-4 0.0 4.09513489783867E-5 3.5525909508985136 0.0 68-69 2.457080938703202E-4 0.0 4.09513489783867E-5 4.006966643488203 0.0 70-71 2.457080938703202E-4 0.0 4.09513489783867E-5 4.5030103336634015 0.0 72-73 2.457080938703202E-4 0.0 4.09513489783867E-5 4.995429829454012 0.0 74-75 2.457080938703202E-4 0.0 4.09513489783867E-5 5.521347528708944 0.0 76-77 2.457080938703202E-4 0.0 4.09513489783867E-5 6.076914004624227 0.0 78-79 2.457080938703202E-4 0.0 4.09513489783867E-5 6.727016669656115 0.0 80-81 2.457080938703202E-4 0.0 4.09513489783867E-5 7.430847504547647 0.0 82-83 2.457080938703202E-4 0.0 4.09513489783867E-5 8.122577215816065 0.0 84-85 2.457080938703202E-4 0.0 4.09513489783867E-5 8.840106276940869 0.0 86-87 2.457080938703202E-4 0.0 4.09513489783867E-5 9.596170557454332 0.0 88-89 2.457080938703202E-4 0.0 4.09513489783867E-5 10.411450488590544 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 8120 0.0 25.448551 1 TACATGG 7915 0.0 25.044682 2 ACATGGG 8145 0.0 23.522333 3 GAGTACT 6440 0.0 21.120792 12-13 AGTACAT 6040 0.0 20.570957 2 CATGGGG 3590 0.0 20.343065 4 GGACCGA 170 8.1027276E-5 19.513165 6 GAGTACA 6915 0.0 19.303852 1 AGTACTT 7075 0.0 18.521786 12-13 GTACTTT 7310 0.0 18.347956 14-15 AGAGTAC 10850 0.0 18.17021 10-11 CATGGGA 4465 0.0 18.162046 4 GTGTAGC 1440 0.0 18.143875 1 TGGACCG 175 0.0021942882 16.259356 5 GTATAGG 630 0.0 15.834655 1 GTAAGAC 765 0.0 15.497751 3 CTGGTCG 585 0.0 15.391336 9 TGTACCG 255 1.0463514E-4 14.877842 5 ACTTTTT 9495 0.0 14.750839 16-17 GTAGCCC 1425 0.0 14.642929 3 >>END_MODULE