FastQCFastQC Report
Fri 27 May 2016
SRR939300_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939300_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2441922
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT186430.7634559990040632No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT90640.371183027140097No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT71580.293129755987292No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68380.28002532431420823No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC64730.2650780819370971No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49700.2035282044225819No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT48020.19664837779421293No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT46070.1886628647434275No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC43880.17969451931716085No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC42690.1748213087887328No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG40470.16573010931553095No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36320.14873529948950048No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC35330.1446811159406402No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA33370.13665465154087642No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA29230.1197007930638243No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG84550.024.8730371
TACATGG84200.024.1753142
ACATGGG85550.022.961873
GAGTACT66050.021.55315812-13
AGTACTT71800.019.76101512-13
CATGGGG36700.019.6518964
CCGTGCG1951.0425032E-519.4674459
GTACTTT74400.019.13422214-15
AGAGTAC116550.018.89138410-11
GTGTAGC13900.018.4841351
CATGGGA47850.018.3449574
GTTCGCA2152.4171693E-517.6565239
AGTACAT65300.017.5844632
TAACACG1350.00946169917.5736984
CCGCGAT1650.001474421517.2552369
GAGTACA73650.017.2487931
ATGGGAT25000.016.322455
ATGGGGG15150.015.9729265
GAGTACG1800.002590664715.8598441
GGACCGT1800.002638584515.8163296
Produced by FastQC (version 0.11.5)