##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939300_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2441922 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.27858301780319 33.0 31.0 34.0 31.0 34.0 2 32.4800616072094 34.0 31.0 34.0 31.0 34.0 3 32.58257184299908 34.0 31.0 34.0 31.0 34.0 4 36.10494806959436 37.0 35.0 37.0 35.0 37.0 5 36.04205949248174 37.0 35.0 37.0 35.0 37.0 6 36.07462277664889 37.0 35.0 37.0 35.0 37.0 7 36.026163407348804 37.0 35.0 37.0 35.0 37.0 8 36.00775946160442 37.0 35.0 37.0 35.0 37.0 9 37.72148987559799 39.0 37.0 39.0 35.0 39.0 10-11 37.64234074634653 39.0 37.0 39.0 35.0 39.0 12-13 37.58101302990022 39.0 37.0 39.0 35.0 39.0 14-15 38.87911530343721 40.0 38.0 41.0 35.0 41.0 16-17 38.846739985961875 40.0 38.0 41.0 35.0 41.0 18-19 38.95428887572986 40.0 38.0 41.0 35.0 41.0 20-21 38.95889672151691 40.0 38.0 41.0 35.0 41.0 22-23 38.90336771608594 40.0 38.0 41.0 35.0 41.0 24-25 38.842486574100235 40.0 38.0 41.0 34.5 41.0 26-27 38.683781259188464 40.0 38.0 41.0 34.0 41.0 28-29 38.560861075824704 40.0 38.0 41.0 34.0 41.0 30-31 38.41251358561002 40.0 38.0 41.0 34.0 41.0 32-33 38.23182517705315 40.0 38.0 41.0 34.0 41.0 34-35 38.16606529610692 40.0 38.0 41.0 34.0 41.0 36-37 38.08888879333574 40.0 38.0 41.0 33.0 41.0 38-39 37.99961300975215 40.0 38.0 41.0 33.0 41.0 40-41 37.880610641945154 40.0 37.5 41.0 33.0 41.0 42-43 37.729858488518474 40.0 37.0 41.0 33.0 41.0 44-45 37.530397572076424 40.0 37.0 41.0 32.5 41.0 46-47 37.300653133064856 40.0 37.0 41.0 32.0 41.0 48-49 37.04268522909413 40.0 37.0 41.0 31.0 41.0 50-51 36.84031779065834 40.0 36.0 41.0 31.0 41.0 52-53 36.66764212779933 39.5 36.0 41.0 31.0 41.0 54-55 36.44547983105112 39.0 36.0 41.0 30.0 41.0 56-57 36.22108404772962 39.0 35.0 40.5 30.0 41.0 58-59 35.968415248316695 39.0 35.0 40.0 29.0 41.0 60-61 35.64620675844683 38.0 35.0 40.0 28.0 41.0 62-63 35.3280874655292 38.0 34.5 40.0 28.0 41.0 64-65 34.953158823254796 38.0 34.0 40.0 28.0 41.0 66-67 34.58973955761077 37.0 34.0 39.5 27.0 41.0 68-69 34.21512153131836 36.5 34.0 39.0 26.0 40.5 70-71 33.83696571798772 36.0 33.5 39.0 26.0 40.0 72-73 33.35168486135102 35.5 33.0 38.5 26.0 40.0 74-75 32.867179213750475 35.0 32.5 37.5 25.0 39.5 76-77 30.797950344032284 33.5 29.5 35.5 21.5 38.5 78-79 31.79724741412707 34.5 31.5 36.0 23.0 39.0 80-81 31.816921261203266 35.0 32.0 36.0 24.0 38.5 82-83 31.49304850851092 35.0 31.5 36.0 22.5 37.0 84-85 31.111860452545166 34.0 31.0 35.0 21.0 37.0 86-87 30.614702885677758 34.0 31.0 35.0 19.0 36.5 88-89 30.112639347202737 34.0 30.5 35.0 17.0 36.0 90-91 29.696670696279405 34.0 30.0 35.0 9.0 35.5 92-93 29.37702371328814 34.0 30.0 35.0 4.5 35.0 94-95 29.02080205674055 34.0 29.0 35.0 2.0 35.0 96-97 28.512546060029763 34.0 29.0 35.0 2.0 35.0 98-99 27.851310156507864 34.0 29.0 35.0 2.0 35.0 100-101 24.634431607561584 31.0 21.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.20278594273651862 1101 2 0.1817218139132848 1101 3 0.1882809033292645 1101 4 0.17111104059516435 1101 5 0.13862603345042146 1101 6 0.12702284868042568 1101 7 0.15623699575645134 1101 8 0.22720448902681056 1101 9 0.2890608427251635 1101 10-11 0.16477443131078928 1101 12-13 0.29907834487350726 1101 14-15 0.26748026602448505 1101 16-17 0.31039255592189363 1101 18-19 0.23754531283829294 1101 20-21 0.32973275638335053 1101 22-23 0.2689355363036796 1101 24-25 0.3440653754280518 1101 26-27 0.33395840026631873 1101 28-29 0.397917636841278 1101 30-31 0.38618613085278497 1101 32-33 0.3391722643022632 1101 34-35 0.35302046598132364 1101 36-37 0.4706639261569947 1101 38-39 0.4452102609294357 1101 40-41 0.5001673988161954 1101 42-43 0.5319768985170299 1101 44-45 0.5668359737548272 1101 46-47 0.5460098088179848 1101 48-49 0.6957087094707077 1101 50-51 0.663976174520478 1101 52-53 0.6988878241417922 1101 54-55 0.680179835842317 1101 56-57 0.6663333972458148 1101 58-59 0.5937358050432309 1101 60-61 0.617848778334249 1101 62-63 0.6434373433769238 1101 64-65 0.6093143822011058 1101 66-67 0.6659292919232342 1101 68-69 0.6298745208672045 1101 70-71 0.6013756381195066 1101 72-73 0.44211967944922037 1101 74-75 0.43798156600268356 1101 76-77 0.4852378563406674 1101 78-79 0.6408577468848513 1101 80-81 0.6968616531054295 1101 82-83 0.7418569142901497 1101 84-85 0.7652520523637456 1101 86-87 0.6918820738670384 1101 88-89 0.8601256380917128 1101 90-91 0.9491897911401352 1101 92-93 0.7995284819134376 1101 94-95 0.8783313452311994 1101 96-97 0.7794990516941205 1101 98-99 0.6739041955627734 1101 100-101 0.5933363602627111 1102 1 0.17906909950747973 1102 2 0.13962934450374576 1102 3 0.1500572029104461 1102 4 0.083805053898935 1102 5 0.09892988497378497 1102 6 0.11243017396580512 1102 7 0.13609779890993678 1102 8 0.19103219700086527 1102 9 0.2941758135718402 1102 10-11 0.3026725178674141 1102 12-13 0.24766646181527818 1102 14-15 0.28937235767266856 1102 16-17 0.3298780613830061 1102 18-19 0.22883586059225536 1102 20-21 0.31387950845693524 1102 22-23 0.3071695019766665 1102 24-25 0.24487982069371128 1102 26-27 0.3097868062776499 1102 28-29 0.2574618595562299 1102 30-31 0.2701803412494357 1102 32-33 0.3292264648442682 1102 34-35 0.459333597294453 1102 36-37 0.4920415232663018 1102 38-39 0.39977316640697325 1102 40-41 0.5197723918358221 1102 42-43 0.4667264263153399 1102 44-45 0.5419287716525005 1102 46-47 0.3917197537962167 1102 48-49 0.452177256731936 1102 50-51 0.42458161921128834 1102 52-53 0.3791128385131515 1102 54-55 0.2690210939518636 1102 56-57 0.3852752548462135 1102 58-59 0.408056450522416 1102 60-61 0.21756094060738462 1102 62-63 0.2554382467192937 1102 64-65 0.3260069173082769 1102 66-67 0.12478266303270402 1102 68-69 0.24104332270673012 1102 70-71 0.24547250105783291 1102 72-73 0.4191431663507572 1102 74-75 0.383284596305721 1102 76-77 0.3847613432422037 1102 78-79 0.394792008197161 1102 80-81 0.5312845405161255 1102 82-83 0.4472451462027678 1102 84-85 0.41790213037968016 1102 86-87 0.6045555566624756 1102 88-89 0.7817797811482912 1102 90-91 0.8569591514094981 1102 92-93 0.7966388128652291 1102 94-95 0.8011538795171482 1102 96-97 0.7618216681021046 1102 98-99 0.8170812917276713 1102 100-101 0.9013941741741824 1103 1 0.12381436761432241 1103 2 0.13425666992019103 1103 3 0.1403117321529379 1103 4 0.05290589287270819 1103 5 0.03406626887521469 1103 6 0.1051775091094811 1103 7 0.142157661918084 1103 8 0.12729977694655048 1103 9 0.17201940778413416 1103 10-11 0.19461654575214737 1103 12-13 0.17483715447527715 1103 14-15 0.3334723860437663 1103 16-17 0.24868708339086965 1103 18-19 0.24094200901360807 1103 20-21 0.3036336931932837 1103 22-23 0.2335292879775679 1103 24-25 0.2576708270569412 1103 26-27 0.29130658498311135 1103 28-29 0.2421179613679314 1103 30-31 0.2763418335689778 1103 32-33 0.2911034153569787 1103 34-35 0.3045115245833969 1103 36-37 0.3968186075284521 1103 38-39 0.4543302733659971 1103 40-41 0.42318827154237937 1103 42-43 0.37451814195591027 1103 44-45 0.42593983637912913 1103 46-47 0.46197712014809866 1103 48-49 0.41629359848202796 1103 50-51 0.5562242042232484 1103 52-53 0.5518157931599248 1103 54-55 0.5201081121498916 1103 56-57 0.5043854157508463 1103 58-59 0.4746619504616163 1103 60-61 0.43193272352799283 1103 62-63 0.6178925489930336 1103 64-65 0.5662762400497385 1103 66-67 0.470102749767193 1103 68-69 0.4089628095952378 1103 70-71 0.5255881132655134 1103 72-73 0.49596661708637413 1103 74-75 0.47602585192927194 1103 76-77 0.6173282179635571 1103 78-79 0.6182156899545497 1103 80-81 0.5374711615188374 1103 82-83 0.6718687225818023 1103 84-85 0.6961139408607693 1103 86-87 0.6425677562328573 1103 88-89 0.8816199910943858 1103 90-91 1.0662783464810346 1103 92-93 0.976073316168435 1103 94-95 0.801709547656408 1103 96-97 0.5895530951681245 1103 98-99 0.42176424475324836 1103 100-101 0.6246532022615661 1104 1 0.049933611527862354 1104 2 0.08321794504505675 1104 3 0.0330535410731585 1104 4 0.03948488195179323 1104 5 0.09556895518553432 1104 6 0.042749390651046326 1104 7 0.09298444116435434 1104 8 0.08398873258436623 1104 9 0.06521466088914707 1104 10-11 0.09368031917293962 1104 12-13 0.14738426152870687 1104 14-15 0.20560942183443132 1104 16-17 0.11103154012842964 1104 18-19 0.07568190421588383 1104 20-21 0.18038324989937848 1104 22-23 0.1467491867402444 1104 24-25 0.127577241486577 1104 26-27 0.11017886168284008 1104 28-29 0.17266087245226913 1104 30-31 0.1546028058251423 1104 32-33 0.2916257643188729 1104 34-35 0.24993620834364805 1104 36-37 0.29962471065684326 1104 38-39 0.38777069864705993 1104 40-41 0.3987423010859388 1104 42-43 0.4736385422204492 1104 44-45 0.641257700167067 1104 46-47 0.5130372191679768 1104 48-49 0.4274713977922744 1104 50-51 0.27839828853146287 1104 52-53 0.4692577199014636 1104 54-55 0.3939478572061219 1104 56-57 0.3737062371738702 1104 58-59 0.5370251656951552 1104 60-61 0.504742917999522 1104 62-63 0.5225923623059785 1104 64-65 0.5028704740667536 1104 66-67 0.5727212874555647 1104 68-69 0.5603224979972907 1104 70-71 0.4752523927948147 1104 72-73 0.4711221505889327 1104 74-75 0.5840702992192703 1104 76-77 0.6547832346607692 1104 78-79 0.713416033033976 1104 80-81 0.6052890780101521 1104 82-83 0.4858277763572012 1104 84-85 0.38405640517668616 1104 86-87 0.4244090831709926 1104 88-89 0.7602151417596623 1104 90-91 0.7701342031528498 1104 92-93 0.7571845062261637 1104 94-95 0.7042736056754038 1104 96-97 0.5872710392213527 1104 98-99 0.7490145029997635 1104 100-101 0.6453784123528159 1105 1 0.20174582398061602 1105 2 0.1837634140270339 1105 3 0.20940474159930034 1105 4 0.16222289354431751 1105 5 0.17984286421012285 1105 6 0.16951587269820578 1105 7 0.13448375427194037 1105 8 0.21595559695420263 1105 9 0.18502308007779078 1105 10-11 0.17300496984656633 1105 12-13 0.16850770141400773 1105 14-15 0.2272088923954314 1105 16-17 0.24496282691687554 1105 18-19 0.2374823338794556 1105 20-21 0.23284793523973235 1105 22-23 0.30714811617684035 1105 24-25 0.27809993889885476 1105 26-27 0.33017038976003477 1105 28-29 0.28262789112945086 1105 30-31 0.2606630770764866 1105 32-33 0.1965660713455506 1105 34-35 0.35197493798968793 1105 36-37 0.3093597439677538 1105 38-39 0.3545723343049474 1105 40-41 0.4065513015488662 1105 42-43 0.41244032319689694 1105 44-45 0.4431858678497207 1105 46-47 0.43615789050981135 1105 48-49 0.22199431093521582 1105 50-51 0.3314524878635936 1105 52-53 0.2604060961537087 1105 54-55 0.2805740918521664 1105 56-57 0.3424503851051739 1105 58-59 0.2823991288022185 1105 60-61 0.3715919694587768 1105 62-63 0.40749556066283077 1105 64-65 0.45455073875628926 1105 66-67 0.5035133186046821 1105 68-69 0.596975549162913 1105 70-71 0.5375657820283593 1105 72-73 0.3536127985149946 1105 74-75 0.5340750932339375 1105 76-77 0.6554991533363008 1105 78-79 0.6092291728289432 1105 80-81 0.6761307571737163 1105 82-83 0.6660928404247137 1105 84-85 0.48247247021947715 1105 86-87 0.6671867877068784 1105 88-89 0.7257235170531082 1105 90-91 0.6534400208394686 1105 92-93 0.7422848584364274 1105 94-95 0.7102393449499154 1105 96-97 0.7325233167528573 1105 98-99 0.8630551232853385 1105 100-101 0.9813000766549358 1106 1 0.07678781742695406 1106 2 0.10610514390300807 1106 3 0.16866295934556064 1106 4 0.08217488774555193 1106 5 0.08101953935955208 1106 6 0.06026618780558124 1106 7 0.07633029957531079 1106 8 0.04642210612979625 1106 9 0.15514288706714296 1106 10-11 0.17174954915899576 1106 12-13 0.18774678195520522 1106 14-15 0.1360116489543728 1106 16-17 0.12200288553417948 1106 18-19 0.04436435760758428 1106 20-21 0.05594577970137493 1106 22-23 0.10600889971819782 1106 24-25 0.12499550070587162 1106 26-27 0.22413939743388056 1106 28-29 0.2514750149495697 1106 30-31 0.15565361222773078 1106 32-33 0.2556714669354463 1106 34-35 0.3461544406084016 1106 36-37 0.3192498043202576 1106 38-39 0.38010613853730746 1106 40-41 0.38423610044005585 1106 42-43 0.3314640788760741 1106 44-45 0.24979482237301198 1106 46-47 0.45922745312324054 1106 48-49 0.3995280228624978 1106 50-51 0.4942994056072436 1106 52-53 0.39358757331410743 1106 54-55 0.3781458420602988 1106 56-57 0.4659104017988014 1106 58-59 0.3686369417584885 1106 60-61 0.2262189764235103 1106 62-63 0.23826760681966874 1106 64-65 0.15282357827173598 1106 66-67 0.2668134793514767 1106 68-69 0.44538412723888143 1106 70-71 0.3387892000426618 1106 72-73 0.42757269374514806 1106 74-75 0.2150622853669759 1106 76-77 0.28394892166127406 1106 78-79 0.4222701459105558 1106 80-81 0.47635402456616305 1106 82-83 0.38228896582939953 1106 84-85 0.4121738310754708 1106 86-87 0.3312722214280761 1106 88-89 0.6774577971933091 1106 90-91 0.6774299509988673 1106 92-93 0.5898952479817687 1106 94-95 0.4255890720953772 1106 96-97 0.6156427205335682 1106 98-99 0.7569190355396955 1106 100-101 0.6042339901507638 1107 1 0.13070119224695986 1107 2 0.1816793750101482 1107 3 0.16753737374632038 1107 4 0.12761988001241775 1107 5 0.15020200211839096 1107 6 0.14666751380716647 1107 7 0.13706332667076992 1107 8 0.12966245935595566 1107 9 0.16612822357794244 1107 10-11 0.17499481805045036 1107 12-13 0.17680618115634417 1107 14-15 0.2778577145976229 1107 16-17 0.136837335330533 1107 18-19 0.19015602532685705 1107 20-21 0.19820455421654515 1107 22-23 0.2912718509699559 1107 24-25 0.23609550098657195 1107 26-27 0.25261780078649565 1107 28-29 0.27736576437624905 1107 30-31 0.28108561885773753 1107 32-33 0.1786895520925853 1107 34-35 0.2535145002840835 1107 36-37 0.3500616574944928 1107 38-39 0.3770916532892201 1107 40-41 0.32530320330622686 1107 42-43 0.32268509013474045 1107 44-45 0.36478340862626624 1107 46-47 0.38495038222819034 1107 48-49 0.38643747637806314 1107 50-51 0.3695000145743208 1107 52-53 0.4246741563898979 1107 54-55 0.4479282019445421 1107 56-57 0.5776692450841594 1107 58-59 0.5231784761477911 1107 60-61 0.4297390980204412 1107 62-63 0.41260267624949165 1107 64-65 0.5210770820428721 1107 66-67 0.39546066791373136 1107 68-69 0.5432214801197901 1107 70-71 0.522060359508103 1107 72-73 0.4630693408474045 1107 74-75 0.5591364877029079 1107 76-77 0.6238469137120006 1107 78-79 0.8041063767145538 1107 80-81 0.6079906112909867 1107 82-83 0.7494911803699402 1107 84-85 0.7045159192753481 1107 86-87 0.7026717860706491 1107 88-89 0.9567324962184571 1107 90-91 1.0001623240757596 1107 92-93 0.7986217292776807 1107 94-95 0.8460738002004895 1107 96-97 0.7843331080044855 1107 98-99 0.9324961788539703 1107 100-101 0.7439948453151217 1108 1 0.022749809041847868 1108 2 -0.002063190603429632 1108 3 0.051018030700539896 1108 4 -0.0179133496487367 1108 5 -3.531896751383101E-4 1108 6 0.03926313774998391 1108 7 -0.06706923730587988 1108 8 0.01780972841253714 1108 9 0.07343301978026773 1108 10-11 -0.011603165798518944 1108 12-13 -0.042294735523960014 1108 14-15 0.03963473756920166 1108 16-17 0.056282348153153805 1108 18-19 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sequence quality scores pass #Quality Count 2 4.0 3 6.0 4 10.0 5 35.0 6 77.0 7 216.0 8 567.0 9 1072.0 10 1852.0 11 3059.0 12 4376.0 13 6366.0 14 7133.0 15 9323.0 16 11974.0 17 16414.0 18 15588.0 19 11053.0 20 10423.0 21 11075.0 22 12235.0 23 13303.0 24 15865.0 25 18825.0 26 22858.0 27 28235.0 28 33972.0 29 42073.0 30 52365.0 31 64487.0 32 80210.0 33 102180.0 34 137328.0 35 195922.0 36 308093.0 37 550418.0 38 587568.0 39 65360.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.79295340336647 20.691970418042875 14.774100634339069 22.740975544251587 2 14.81562474149461 22.199357719042624 39.84881580984159 23.136201729621174 3 19.47912341180431 25.59868824638952 30.85352439594713 24.06866394585904 4 12.328116950500466 17.54232936187151 39.306742803414686 30.822810884213336 5 12.523291079731457 38.71626530249533 35.469109987952116 13.2913336298211 6 29.872944344659658 38.17214472861951 17.958763629632724 13.996147297088113 7 26.532460905794697 33.59251442101755 22.41140380405271 17.46362086913505 8 24.354831972520007 35.577262500604036 21.00103934523707 19.06686618163889 9 24.957758683528795 17.778495791429865 22.164590023759974 35.099155501281366 10-11 23.93813152099043 27.05524582685278 28.826821659332282 20.179800992824504 12-13 25.279800091894828 24.839900701168997 28.54196407583862 21.33833513109755 14-15 21.83760334614532 26.49492424712447 27.74385205998146 23.923620346748752 16-17 20.857342935126468 29.56005659515829 28.199008560939102 21.38359190877614 18-19 20.517383438127833 29.219893182501327 29.885434506098065 20.377288873272775 20-21 21.817138303352852 27.615050767387327 29.46723933033078 21.10057159892904 22-23 21.152518385108124 27.879780762858108 29.400611485542942 21.567089366490823 24-25 21.644034202594682 27.774538068405192 29.6264210129734 20.955006716026734 26-27 21.403087686479303 27.519121435705824 30.255707354908672 20.822083522906205 28-29 20.691836184775763 28.854525246916157 29.70682519752883 20.746813370779247 30-31 21.264274616470143 27.76999019624705 29.488718312869942 21.47701687441286 32-33 21.026838695093456 28.07243638412693 29.657192162567025 21.243532758212588 34-35 21.980958894432334 27.69042385349467 29.455355579695087 20.873261672377907 36-37 21.546597655555583 27.3699426463926 29.925622996494557 21.157836701557255 38-39 21.041589961826073 28.211006262391376 29.86002893862889 20.88737483715366 40-41 21.256503843426895 28.115260618626458 29.506488176449892 21.121747361496755 42-43 21.01697150365749 28.163653448861574 28.911983706897804 21.90739134058313 44-45 21.498196590568213 27.74396193132199 28.956807243532452 21.801034234577344 46-47 21.866565926162533 28.221612728521745 28.745217641464137 21.16660370385158 48-49 21.66662162018279 27.994895004836355 28.85020487959894 21.488278495381916 50-51 20.78180629848128 28.320929169727783 29.369447508970392 21.52781702282055 52-53 21.444890541139316 28.698787266751353 28.289642339108294 21.566679853001038 54-55 20.963220774455532 27.98256864879386 28.760685230732186 22.293525346018424 56-57 21.59903141869396 28.010906982286905 28.569299101281697 21.82076249773744 58-59 22.119165349903426 27.803344063324214 28.493500680512522 21.583989906259834 60-61 21.483694727407983 28.160931190461714 29.0990262150198 21.25634786711051 62-63 20.9883618788569 28.22300686067221 29.06321704221609 21.7254142182548 64-65 20.728549069134885 28.69733349386262 28.614816525671174 21.95930091133132 66-67 21.20569371175656 28.094161074759967 28.068095541135218 22.632049672348256 68-69 20.87155991480436 28.131208001608577 28.482715776877843 22.51451630670922 70-71 21.521264806984007 27.884531119339602 28.389543154941066 22.204660918735325 72-73 21.349678654764567 27.39346301806528 29.049576522100214 22.20728180506994 74-75 20.816451139716992 28.715495417134534 28.153724811849028 22.314328631299443 76-77 20.933955014966006 27.776099381492724 28.359326127540243 22.93061947600103 78-79 21.01678513891926 27.963526271518912 28.426481271719574 22.593207317842257 80-81 21.36035285804409 27.795188027626978 28.17081942930646 22.673639685022472 82-83 21.116358343960208 27.642406268504892 28.673847895223513 22.567387492311383 84-85 20.81016510764881 27.964959568733157 28.378998182579135 22.845877141038905 86-87 20.705005549651187 28.039932891752596 28.350569876923803 22.90449168167242 88-89 20.70542366911305 27.891367558024427 27.939085472402216 23.464123300460308 90-91 20.90418341373433 27.357067142674534 28.143129527051435 23.595619916539697 92-93 21.240563785411656 27.76552649920841 28.183885480371607 22.810024235008324 94-95 20.877452448819504 27.466894410815417 28.241509811023818 23.414143329341258 96-97 20.238443324561555 27.559356932776723 28.7036195259308 23.498580216730918 98-99 20.61447908655559 27.78940113566281 27.755698175453595 23.840421602328004 100-101 20.36728953151737 27.112486079063753 28.40933685051755 24.110887538901327 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 451.0 1 319.5 2 277.5 3 353.5 4 298.0 5 319.5 6 533.0 7 1018.0 8 1625.0 9 1875.5 10 1747.5 11 1682.5 12 1761.0 13 1919.0 14 2129.0 15 2286.0 16 2434.5 17 2637.5 18 2926.5 19 3237.5 20 3674.5 21 4392.0 22 5350.0 23 6750.5 24 8576.0 25 10761.0 26 14216.5 27 18549.0 28 22512.0 29 27224.5 30 33397.0 31 39333.5 32 45713.0 33 52915.0 34 60113.0 35 66816.5 36 74196.5 37 80926.5 38 82847.5 39 83352.5 40 85125.0 41 87840.0 42 90266.5 43 92334.5 44 94546.0 45 95409.5 46 94933.0 47 105561.5 48 129745.0 49 125697.0 50 97794.0 51 80684.5 52 69839.5 53 62369.5 54 56308.5 55 49532.0 56 43088.0 57 36927.5 58 31148.0 59 26377.0 60 22250.5 61 18232.0 62 14608.0 63 11695.5 64 9290.0 65 7575.5 66 6239.0 67 5242.0 68 4469.5 69 3688.5 70 2866.5 71 2240.5 72 1778.0 73 1395.0 74 1133.0 75 881.0 76 600.0 77 369.5 78 238.0 79 158.5 80 89.0 81 53.5 82 39.5 83 17.0 84 7.0 85 5.5 86 3.5 87 4.0 88 2.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2780187082142673 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0027642160560411018 16-17 6.961729326325739E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 8.19026979567734E-5 26-27 0.0011875891203732142 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0040746592233494765 36-37 0.023587977011550736 38-39 0.0083131238426125 40-41 0.019697598858604 42-43 0.006245080719203972 44-45 7.985513050785405E-4 46-47 1.8428107040274012E-4 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 5.118918622298338E-4 60-61 0.0023956539152356214 62-63 0.0013513945162867609 64-65 0.0 66-67 0.0 68-69 5.32367536719027E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.23481503504206933 78-79 0.0 80-81 0.0013718701907759544 82-83 0.0 84-85 0.0 86-87 0.10563400468974848 88-89 0.36338179515971436 90-91 0.3648355680484471 92-93 0.0 94-95 2.047567448919335E-5 96-97 0.0 98-99 0.0 100-101 2.661837683595135E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2441922.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.163658051443704 #Duplication Level Percentage of deduplicated Percentage of total 1 67.45438195317236 30.46486640604552 2 15.299439835855683 13.81957338250444 3 6.59517242074778 8.935863360028948 4 3.4828196891870924 6.291875099891252 5 2.045288380512098 4.618635251701973 6 1.2900349127469104 3.4957617404235286 7 0.8622480796245028 2.725959419657254 8 0.5852953946648161 2.114726485098123 9 0.4324750132934812 1.7578938254562317 >10 1.7963965803329065 13.616570507244738 >50 0.10055618046654674 3.1441259185932937 >100 0.05216424927444621 4.101360824507919 >500 0.0013636500444419142 0.4337397002669239 >1k 0.0019091100622186392 2.5010242429059955 >5k 3.636400118511694E-4 1.212574268078648 >10k+ 9.091000296279235E-5 0.7654495675952404 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 18643 0.7634559990040632 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 9064 0.371183027140097 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 7158 0.293129755987292 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6838 0.28002532431420823 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 6473 0.2650780819370971 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4970 0.2035282044225819 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 4802 0.19664837779421293 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 4607 0.1886628647434275 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 4388 0.17969451931716085 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 4269 0.1748213087887328 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 4047 0.16573010931553095 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3632 0.14873529948950048 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 3533 0.1446811159406402 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 3337 0.13665465154087642 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2923 0.1197007930638243 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 4.09513489783867E-5 0.0 0.0 0.0 0.0 7 4.09513489783867E-5 0.0 0.0 0.0 0.0 8 4.09513489783867E-5 0.0 0.0 0.0 0.0 9 4.09513489783867E-5 0.0 0.0 0.0 0.0 10-11 4.09513489783867E-5 0.0 0.0 0.0 0.0 12-13 4.09513489783867E-5 0.0 0.0 0.0 0.0 14-15 4.09513489783867E-5 0.0 0.0 4.09513489783867E-5 0.0 16-17 4.09513489783867E-5 0.0 0.0 8.19026979567734E-5 0.0 18-19 4.09513489783867E-5 0.0 0.0 8.19026979567734E-5 0.0 20-21 4.09513489783867E-5 0.0 0.0 1.228540469351601E-4 0.0 22-23 4.09513489783867E-5 0.0 0.0 2.457080938703202E-4 0.0 24-25 4.09513489783867E-5 0.0 0.0 6.142702346758005E-4 0.0 26-27 4.09513489783867E-5 0.0 0.0 0.0013309188417975678 0.0 28-29 4.09513489783867E-5 0.0 0.0 0.004688929458025277 0.0 30-31 4.09513489783867E-5 0.0 0.0 0.017240517919900798 0.0 32-33 4.09513489783867E-5 0.0 0.0 0.04518981359764972 0.0 34-35 6.142702346758005E-5 0.0 0.0 0.08845491379331527 0.0 36-37 8.19026979567734E-5 0.0 0.0 0.15680271523824266 0.0 38-39 8.19026979567734E-5 0.0 0.0 0.2601843957341799 0.0 40-41 8.19026979567734E-5 0.0 0.0 0.36854166513099107 0.0 42-43 8.19026979567734E-5 0.0 0.0 0.48547824213877433 2.047567448919335E-5 44-45 8.19026979567734E-5 0.0 0.0 0.6318588390620175 4.09513489783867E-5 46-47 8.19026979567734E-5 0.0 0.0 0.8093829368833239 4.09513489783867E-5 48-49 8.19026979567734E-5 0.0 0.0 0.9895688723882253 4.09513489783867E-5 50-51 8.19026979567734E-5 0.0 0.0 1.1882648176313575 4.09513489783867E-5 52-53 8.19026979567734E-5 0.0 0.0 1.3844627305868085 4.09513489783867E-5 54-55 8.19026979567734E-5 0.0 0.0 1.6111079715076895 4.09513489783867E-5 56-57 8.19026979567734E-5 0.0 0.0 1.8897819013056107 4.09513489783867E-5 58-59 8.19026979567734E-5 0.0 0.0 2.182502143803119 4.09513489783867E-5 60-61 8.19026979567734E-5 0.0 0.0 2.4955547310683963 4.09513489783867E-5 62-63 8.19026979567734E-5 0.0 0.0 2.8218550797281816 4.09513489783867E-5 64-65 8.19026979567734E-5 0.0 0.0 3.171292940560755 4.09513489783867E-5 66-67 8.19026979567734E-5 0.0 0.0 3.578799814244681 4.09513489783867E-5 68-69 8.19026979567734E-5 0.0 0.0 4.03471118242106 4.09513489783867E-5 70-71 1.0237837244596674E-4 0.0 0.0 4.534379066980845 4.09513489783867E-5 72-73 1.228540469351601E-4 0.0 0.0 5.0294399247805615 4.09513489783867E-5 74-75 1.228540469351601E-4 0.0 0.0 5.557302813111967 4.09513489783867E-5 76-77 1.228540469351601E-4 0.0 0.0 6.115162564570039 4.09513489783867E-5 78-79 1.228540469351601E-4 0.0 0.0 6.768582288869178 4.09513489783867E-5 80-81 1.228540469351601E-4 0.0 0.0 7.4770201505207785 4.09513489783867E-5 82-83 1.228540469351601E-4 0.0 0.0 8.172025969707468 4.09513489783867E-5 84-85 1.228540469351601E-4 0.0 0.0 8.893670641404597 4.09513489783867E-5 86-87 1.228540469351601E-4 0.0 0.0 9.65331816495367 8.19026979567734E-5 88-89 1.228540469351601E-4 0.0 0.0 10.473041317454038 8.19026979567734E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 8455 0.0 24.873037 1 TACATGG 8420 0.0 24.175314 2 ACATGGG 8555 0.0 22.96187 3 GAGTACT 6605 0.0 21.553158 12-13 AGTACTT 7180 0.0 19.761015 12-13 CATGGGG 3670 0.0 19.651896 4 CCGTGCG 195 1.0425032E-5 19.467445 9 GTACTTT 7440 0.0 19.134222 14-15 AGAGTAC 11655 0.0 18.891384 10-11 GTGTAGC 1390 0.0 18.484135 1 CATGGGA 4785 0.0 18.344957 4 GTTCGCA 215 2.4171693E-5 17.656523 9 AGTACAT 6530 0.0 17.584463 2 TAACACG 135 0.009461699 17.573698 4 CCGCGAT 165 0.0014744215 17.255236 9 GAGTACA 7365 0.0 17.248793 1 ATGGGAT 2500 0.0 16.32245 5 ATGGGGG 1515 0.0 15.972926 5 GAGTACG 180 0.0025906647 15.859844 1 GGACCGT 180 0.0026385845 15.816329 6 >>END_MODULE