FastQCFastQC Report
Fri 27 May 2016
SRR939299_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939299_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences619865
Sequences flagged as poor quality0
Sequence length101
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109391.7647390964161551No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80281.295120711767885No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66901.0792672598065707No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA53140.8572834407491953No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28610.4615521121534527No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA21010.338944770232228No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19940.3216829470933187No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA16920.2729626612246215No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15880.25618481443540125No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14710.2373097367975285No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT14450.23311527510022342No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12460.20101151057085012No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT12360.19939825607188663No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11460.18487896558121525No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG8510.1372879578617925No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT8400.13551337791293264No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT7750.12502722366967No Hit
TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC7690.12405927097029193No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA6680.10776540053076074No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC6530.1053455187823155No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG6390.10308696248376661No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACAGG1054.7539288E-836.1011246
TAGACCC702.0723887E-433.872185
GCTTCGG702.0862142E-433.8338669
TGGGAGT2550.029.7303416
CATATAG1601.9354047E-929.638163
GTATGAC650.005889686329.1821883
CATGGGT3600.028.979534
CATTCCG856.509331E-427.8631849
GTCCTGG2053.274181E-1127.7698971
ATGACCG700.0084569627.0977445
GGTACTC1057.017868E-527.0758448
AGATAGT700.00849038327.0758426
GATAGTC700.00849038327.0758427
GTACAGG1257.456212E-626.5665381
GGGATAG1257.526809E-626.5343287
GGGATGC1806.864866E-926.3237367
GTACATG63950.025.8899841
ATGGGAT18350.025.5841125
GGATGAC2601.8189894E-1225.5137778
GGTATCA78050.025.3460331
Produced by FastQC (version 0.11.5)