##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939299_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 619865 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.973471643019046 33.0 31.0 34.0 30.0 34.0 2 32.0194526227485 33.0 31.0 34.0 30.0 34.0 3 32.00642720592387 34.0 31.0 34.0 30.0 34.0 4 35.55312689053262 37.0 35.0 37.0 33.0 37.0 5 35.60257798068935 37.0 35.0 37.0 33.0 37.0 6 35.73293217071459 37.0 35.0 37.0 35.0 37.0 7 35.69637905027707 37.0 35.0 37.0 35.0 37.0 8 35.68152742935962 37.0 35.0 37.0 33.0 37.0 9 37.28641397723698 39.0 37.0 39.0 34.0 39.0 10-11 37.205954522355675 39.0 37.0 39.0 34.0 39.0 12-13 37.117320706928126 39.0 37.0 39.0 33.5 39.0 14-15 38.23156655078122 40.0 38.0 41.0 33.0 41.0 16-17 38.20143982964032 40.0 38.0 41.0 33.5 41.0 18-19 38.26947561162511 40.0 38.0 41.0 34.0 41.0 20-21 38.29278149274438 40.0 38.0 41.0 34.0 41.0 22-23 38.258137659006394 40.0 38.0 41.0 34.0 41.0 24-25 38.17261903801634 40.0 38.0 41.0 34.0 41.0 26-27 38.045239689287186 40.0 38.0 41.0 33.5 41.0 28-29 37.87491227928662 40.0 37.5 41.0 33.0 41.0 30-31 37.58377872601292 40.0 37.0 41.0 33.0 41.0 32-33 37.23180047268357 40.0 35.5 41.0 33.0 41.0 34-35 36.91807248352464 39.0 35.0 41.0 31.5 41.0 36-37 36.64580432836182 39.0 35.0 41.0 31.0 41.0 38-39 36.06463665475547 38.5 35.0 40.5 30.5 41.0 40-41 36.01088220822275 38.5 35.0 40.0 30.0 41.0 42-43 36.00259330660708 39.0 35.0 41.0 30.0 41.0 44-45 35.73694756116251 39.0 35.0 41.0 29.0 41.0 46-47 35.26937962298243 39.0 35.0 41.0 25.0 41.0 48-49 34.908994700458976 39.0 35.0 41.0 23.5 41.0 50-51 33.752967178337215 38.0 34.0 40.0 21.5 40.5 52-53 33.878717140022424 38.0 34.0 39.5 22.0 40.5 54-55 34.37996902551362 38.5 35.0 40.5 22.0 41.0 56-57 34.33964653593928 38.5 35.0 41.0 21.0 41.0 58-59 34.16731304396925 38.0 35.0 40.5 20.0 41.0 60-61 33.890769764384174 38.0 34.0 40.0 18.5 41.0 62-63 33.50603841158962 37.5 34.0 40.0 14.0 41.0 64-65 33.12301388205496 37.0 33.5 40.0 8.5 41.0 66-67 32.75476353722182 36.5 33.0 39.5 4.5 41.0 68-69 32.345579279359214 36.0 33.0 39.0 2.0 41.0 70-71 31.92115379961766 35.5 32.5 39.0 2.0 40.5 72-73 31.44231727876231 35.0 32.0 38.0 2.0 40.0 74-75 30.958898308502658 35.0 31.0 37.0 2.0 39.5 76-77 30.477470094294723 35.0 31.0 37.0 2.0 39.0 78-79 30.06433013640067 35.0 31.0 36.0 2.0 39.0 80-81 29.647741040387828 35.0 30.5 36.0 2.0 37.5 82-83 29.177894380227954 35.0 30.0 35.5 2.0 37.0 84-85 28.700877610447435 34.0 29.0 35.0 2.0 37.0 86-87 28.250594887596492 34.0 29.0 35.0 2.0 36.0 88-89 27.76913844143483 34.0 28.0 35.0 2.0 36.0 90-91 27.299413581989626 34.0 26.5 35.0 2.0 35.5 92-93 26.82871270357255 34.0 25.5 35.0 2.0 35.0 94-95 26.33080751453946 34.0 24.5 35.0 2.0 35.0 96-97 25.73944165261791 33.0 22.0 35.0 2.0 35.0 98-99 25.010297403466883 33.0 10.0 35.0 2.0 35.0 100-101 22.546677905673008 29.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.13508967413669382 1101 2 0.15164767040883476 1101 3 0.2504753920983376 1101 4 0.2209703759993289 1101 5 0.03452536683248297 1101 6 0.23397027988266217 1101 7 0.04633791478782712 1101 8 -0.09573107040555584 1101 9 0.09818505122510146 1101 10-11 0.30823979890532627 1101 12-13 0.26924771501020217 1101 14-15 0.292282309733082 1101 16-17 0.2918056404524876 1101 18-19 0.3582397508985835 1101 20-21 0.39366946236243905 1101 22-23 0.37910806948922016 1101 24-25 0.4086277245141048 1101 26-27 0.36858620584221313 1101 28-29 0.3885069625574644 1101 30-31 0.7134759408141989 1101 32-33 0.6232995819699383 1101 34-35 0.7405783209361516 1101 36-37 0.6864398152360351 1101 38-39 0.9587697595050173 1101 40-41 0.6867893476303024 1101 42-43 0.548381195743552 1101 44-45 0.4093228660826682 1101 46-47 0.462187538159057 1101 48-49 0.38044837317939795 1101 50-51 0.3977704096533685 1101 52-53 0.39460020030916354 1101 54-55 0.5579346697268122 1101 56-57 0.5819891727634428 1101 58-59 0.6041975777472004 1101 60-61 0.6482974532289631 1101 62-63 0.7119341175952556 1101 64-65 0.3415270681084479 1101 66-67 0.37414805769677173 1101 68-69 0.49661361337243193 1101 70-71 0.6165290300645836 1101 72-73 0.7900140139534244 1101 74-75 0.44735775086447305 1101 76-77 0.14806779718771068 1101 78-79 0.1582086928536306 1101 80-81 -0.0050430479152545615 1101 82-83 0.04032089389701099 1101 84-85 -0.32524414474565333 1101 86-87 -0.12101743091670514 1101 88-89 -0.28045089454228744 1101 90-91 0.05580919130035511 1101 92-93 -0.1251164555963591 1101 94-95 -0.02811311975624875 1101 96-97 -0.041564878845040454 1101 98-99 -0.0674610020819344 1101 100-101 -0.318258862495874 1102 1 0.2678812861943598 1102 2 0.1625869452013191 1102 3 0.24338059087509123 1102 4 0.15477553108888742 1102 5 0.31260313004786155 1102 6 0.2763119137839283 1102 7 0.019933371319062587 1102 8 0.15000069018859108 1102 9 0.17680453571614407 1102 10-11 0.35052900816700827 1102 12-13 0.2942367932102954 1102 14-15 0.457087901694635 1102 16-17 0.4027886024446232 1102 18-19 0.27332930965787483 1102 20-21 0.3382478808858096 1102 22-23 0.480130349961037 1102 24-25 0.22909954627032647 1102 26-27 0.1946150393939874 1102 28-29 6.083168606494382E-4 1102 30-31 0.06285121385919723 1102 32-33 0.11408158284368852 1102 34-35 0.06915848694750792 1102 36-37 0.17400643821550688 1102 38-39 0.4624569591555172 1102 40-41 0.33221529782689885 1102 42-43 0.27973986765268677 1102 44-45 0.20832679793063846 1102 46-47 0.19873624938666978 1102 48-49 0.19771355448127537 1102 50-51 0.2943802829604891 1102 52-53 0.48853641699766825 1102 54-55 0.5453003315878888 1102 56-57 0.5757768529731493 1102 58-59 0.7498681762618418 1102 60-61 0.7225045305553053 1102 62-63 0.550142942409586 1102 64-65 0.6732177627349287 1102 66-67 0.6375119720698592 1102 68-69 0.6314672612535972 1102 70-71 0.5311642419475078 1102 72-73 0.46259592745276734 1102 74-75 0.3683101318168518 1102 76-77 0.29372092082248713 1102 78-79 0.5432327208134389 1102 80-81 0.559631482447351 1102 82-83 0.5694602560638948 1102 84-85 0.6845417879760589 1102 86-87 0.4670122763790552 1102 88-89 0.4112660124039742 1102 90-91 0.5031224285218485 1102 92-93 0.5049665500829725 1102 94-95 0.20321060239316324 1102 96-97 -0.016811711523065043 1102 98-99 0.4030676130338371 1102 100-101 0.013492994210110965 1103 1 0.28260847112917276 1103 2 0.20939203131108997 1103 3 0.3343350412211379 1103 4 0.21365208025997617 1103 5 0.25863564252170335 1103 6 0.2588324352711311 1103 7 0.4066386666675257 1103 8 0.2758980022761506 1103 9 0.34319758255342236 1103 10-11 0.24483127760206713 1103 12-13 0.15107728894503936 1103 14-15 0.44200662051002837 1103 16-17 0.4906527582469735 1103 18-19 0.5496933849838044 1103 20-21 0.5803611916857463 1103 22-23 0.6284564404165351 1103 24-25 0.47657258666949076 1103 26-27 0.4326964815314369 1103 28-29 0.4485320252141065 1103 30-31 0.65939072778162 1103 32-33 0.39974068472682944 1103 34-35 0.5742124061491936 1103 36-37 0.12438467739142567 1103 38-39 0.5627547219110269 1103 40-41 0.44350613961024976 1103 42-43 0.23667693509192844 1103 44-45 0.11989930718542752 1103 46-47 0.17349079630442077 1103 48-49 0.0702228092696302 1103 50-51 0.03082805376364206 1103 52-53 0.15743228050965996 1103 54-55 0.41978930631830025 1103 56-57 0.15787889707422664 1103 58-59 0.14565121183241558 1103 60-61 0.12175609984550562 1103 62-63 0.3009316113295952 1103 64-65 0.21869498790795205 1103 66-67 0.26484981208273695 1103 68-69 0.25997200936240716 1103 70-71 -0.00702986717852383 1103 72-73 0.09637992874039014 1103 74-75 0.03585399146597368 1103 76-77 0.10922067939322488 1103 78-79 0.1097625775654123 1103 80-81 -0.02774981483254635 1103 82-83 -0.09940341687993381 1103 84-85 -0.14191081141231976 1103 86-87 -0.07878685447560585 1103 88-89 0.028045346059215603 1103 90-91 -0.16148404178235154 1103 92-93 0.05824194039361075 1103 94-95 0.025896905306410645 1103 96-97 -0.1332691394966723 1103 98-99 0.14128586508598673 1103 100-101 0.02680379414573153 1104 1 0.15641421105625852 1104 2 0.10782461365504759 1104 3 0.1862717212872056 1104 4 0.01573989304237955 1104 5 0.2502438815918495 1104 6 0.22534641115222342 1104 7 0.14359918519296855 1104 8 0.26493542709931006 1104 9 0.3530882060927283 1104 10-11 0.3165710177161216 1104 12-13 0.35375249383114493 1104 14-15 0.2723110672396416 1104 16-17 0.33092261583944804 1104 18-19 0.43599328215123023 1104 20-21 0.6166906921687527 1104 22-23 0.5203311093268255 1104 24-25 0.5490337128419398 1104 26-27 0.523653447659008 1104 28-29 0.650107233216346 1104 30-31 0.7525134101536253 1104 32-33 0.892757418390481 1104 34-35 0.7744056067350229 1104 36-37 0.6050459219771369 1104 38-39 0.9350008345282745 1104 40-41 0.8190958688178185 1104 42-43 0.9024165505604387 1104 44-45 0.8662830284780014 1104 46-47 0.8927313933565557 1104 48-49 0.8297877963376479 1104 50-51 0.6822988711267683 1104 52-53 0.9807830642184925 1104 54-55 0.7377164510006011 1104 56-57 0.6560871174475409 1104 58-59 0.7186592203083109 1104 60-61 0.8971276993593946 1104 62-63 0.8721743273558857 1104 64-65 0.7590505393086602 1104 66-67 0.7203529126405286 1104 68-69 0.6036135287915343 1104 70-71 0.8440685716361003 1104 72-73 0.9626757749277957 1104 74-75 0.7036564906090383 1104 76-77 0.5577015618836434 1104 78-79 0.7011791741189626 1104 80-81 0.6625776913218253 1104 82-83 0.8321926692501371 1104 84-85 0.7414225219210131 1104 86-87 0.5971082563031196 1104 88-89 0.6272273597078986 1104 90-91 0.8557076942183954 1104 92-93 0.791936745790764 1104 94-95 0.7461066214261933 1104 96-97 0.7639091971086067 1104 98-99 0.9633247544942272 1104 100-101 0.8401408668452426 1105 1 0.4440756104801409 1105 2 0.4091556891603716 1105 3 0.4303717651553285 1105 4 0.3688016121207198 1105 5 0.4882238617745003 1105 6 0.4208540696680032 1105 7 0.3873495111362075 1105 8 0.3626355715880294 1105 9 0.6249356063694336 1105 10-11 0.6094981260703847 1105 12-13 0.5885420648745026 1105 14-15 0.7522329635697531 1105 16-17 0.7073694203686003 1105 18-19 0.4897670146538289 1105 20-21 0.6385695363816879 1105 22-23 0.5619306700320266 1105 24-25 0.7465798587356645 1105 26-27 0.6645299512160108 1105 28-29 0.6830788845905289 1105 30-31 0.6931058445891836 1105 32-33 0.6633439935169392 1105 34-35 0.8017280865418783 1105 36-37 0.8701259736372222 1105 38-39 1.128589646008841 1105 40-41 0.6828071122491011 1105 42-43 0.891549219429713 1105 44-45 0.7451333768154669 1105 46-47 0.7297720434637682 1105 48-49 0.8324444254863366 1105 50-51 0.8832823760979807 1105 52-53 0.8375659047256434 1105 54-55 1.3494866065637297 1105 56-57 1.13778981426357 1105 58-59 1.0984191420205747 1105 60-61 1.4590845245341697 1105 62-63 1.5438413468081862 1105 64-65 1.5416701719821262 1105 66-67 1.2154952079558399 1105 68-69 1.3726708549218998 1105 70-71 1.3071779321124524 1105 72-73 1.511005871836474 1105 74-75 1.3957712717132331 1105 76-77 1.1968218065634844 1105 78-79 1.4752429884371487 1105 80-81 1.5767679562791699 1105 82-83 1.8897657495594835 1105 84-85 1.8476401385013226 1105 86-87 1.781375858005081 1105 88-89 1.7387398283790034 1105 90-91 1.8669860972959142 1105 92-93 1.4106644474384247 1105 94-95 1.6919954418030905 1105 96-97 1.8936238702297175 1105 98-99 2.2696423781302535 1105 100-101 1.76399132255224 1106 1 0.25492585818872726 1106 2 0.313992486918913 1106 3 0.29103750564527076 1106 4 0.44414230244275643 1106 5 0.3031224920231992 1106 6 0.3119787918878032 1106 7 0.310540918080207 1106 8 0.4623481073026241 1106 9 0.45447831389272864 1106 10-11 0.5506713415054989 1106 12-13 0.646996048745045 1106 14-15 0.7323915203650557 1106 16-17 0.6902060259017802 1106 18-19 0.7711587262458934 1106 20-21 0.636508938793817 1106 22-23 0.6153547570343889 1106 24-25 0.7240312417388637 1106 26-27 0.9079309420503563 1106 28-29 0.5814644828337023 1106 30-31 0.5500566844101229 1106 32-33 0.5152372676681622 1106 34-35 0.2981821111875007 1106 36-37 0.3605729558447237 1106 38-39 1.0508985638091985 1106 40-41 0.9117038260692354 1106 42-43 0.8016824083027743 1106 44-45 0.7406117121438598 1106 46-47 0.8188903406965977 1106 48-49 0.5568267558984203 1106 50-51 0.6800943476602725 1106 52-53 0.8002727290171023 1106 54-55 0.5290789723045108 1106 56-57 0.8153594449870099 1106 58-59 0.9322374075071025 1106 60-61 0.937514509360021 1106 62-63 0.7333522497722456 1106 64-65 1.0231845000525652 1106 66-67 0.9832767816990184 1106 68-69 0.9029944051495065 1106 70-71 0.7013840850714104 1106 72-73 0.7373905497279338 1106 74-75 1.0930993392367476 1106 76-77 1.0430007450717547 1106 78-79 1.2938739943874005 1106 80-81 1.1951962518518684 1106 82-83 0.8925894235685767 1106 84-85 0.840073449408365 1106 86-87 1.1134160389299161 1106 88-89 1.1398719192375246 1106 90-91 1.084091532904786 1106 92-93 1.2773182990812266 1106 94-95 1.3323301469264806 1106 96-97 1.3580432477108069 1106 98-99 1.3035162724703824 1106 100-101 1.017552588560097 1107 1 0.17652055343510398 1107 2 0.19853127684009308 1107 3 0.32386257572784416 1107 4 0.30084421244828263 1107 5 0.3718374677633207 1107 6 0.22485573260979663 1107 7 0.2687365608375245 1107 8 0.16637216830511647 1107 9 0.29153530893722746 1107 10-11 0.4552895719647907 1107 12-13 0.367970693700876 1107 14-15 0.37620722703441345 1107 16-17 0.1820848927026617 1107 18-19 0.2647048635995688 1107 20-21 0.18024073707587718 1107 22-23 0.10361303140936684 1107 24-25 0.3076399562242287 1107 26-27 0.36006093466750855 1107 28-29 0.2499732291933583 1107 30-31 0.2547114204664851 1107 32-33 0.45389424309772153 1107 34-35 0.39979979951143463 1107 36-37 0.5584945886801478 1107 38-39 1.0215238175094754 1107 40-41 0.9601472982418855 1107 42-43 0.7735431312770586 1107 44-45 0.8533431753372227 1107 46-47 1.183947673554087 1107 48-49 1.1680489578397584 1107 50-51 1.1165364685809749 1107 52-53 1.0252425574135486 1107 54-55 1.1533096985751925 1107 56-57 0.9011121822257167 1107 58-59 1.0532044244277188 1107 60-61 1.1690267400971877 1107 62-63 1.128447695562791 1107 64-65 0.9981114207509592 1107 66-67 0.8987145628125504 1107 68-69 1.12299102701963 1107 70-71 1.1760413002392127 1107 72-73 1.0445674411401313 1107 74-75 0.7154829912675815 1107 76-77 0.7546759978185307 1107 78-79 0.9897741978924834 1107 80-81 1.1651731225593949 1107 82-83 1.0016948457394612 1107 84-85 0.9724402925187512 1107 86-87 1.0380101865923343 1107 88-89 1.0904422510842977 1107 90-91 1.2088671188332398 1107 92-93 0.9710186317180458 1107 94-95 0.8944809259395754 1107 96-97 1.0981043264784454 1107 98-99 1.2848153268664468 1107 100-101 0.8654768094788956 1108 1 0.08673320302160192 1108 2 0.24127895866080706 1108 3 0.16874080484650733 1108 4 0.2792298177070407 1108 5 0.25869613858945684 1108 6 0.25345378488340486 1108 7 0.10910250651793518 1108 8 0.16409576942128723 1108 9 0.21407697539288506 1108 10-11 0.31783078263423903 1108 12-13 0.1527079329531844 1108 14-15 0.4064321828484765 1108 16-17 0.2817653359294212 1108 18-19 0.34568414558436444 1108 20-21 0.40921506618390424 1108 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-1.3912479517753908 2316 56-57 -1.3820130363676242 2316 58-59 -1.1457373756155889 2316 60-61 -1.175564844267349 2316 62-63 -1.2645300453895274 2316 64-65 -1.6093344930108415 2316 66-67 -1.6213502240901718 2316 68-69 -1.513289430319272 2316 70-71 -1.3256408275691882 2316 72-73 -0.9459175029832494 2316 74-75 -0.8854269815852085 2316 76-77 -1.0953845837646696 2316 78-79 -1.1662525246230508 2316 80-81 -0.9851878687301792 2316 82-83 -1.1014562293234142 2316 84-85 -1.0910517048831103 2316 86-87 -1.3378006906123368 2316 88-89 -1.4240775513905923 2316 90-91 -1.5154721047232087 2316 92-93 -1.7212050662983778 2316 94-95 -1.470417979844612 2316 96-97 -1.3152369456098931 2316 98-99 -1.8135974772905747 2316 100-101 -1.4232527260206673 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2734.0 3 465.0 4 437.0 5 470.0 6 545.0 7 664.0 8 817.0 9 1088.0 10 1303.0 11 1782.0 12 2554.0 13 3766.0 14 4097.0 15 4988.0 16 6987.0 17 9468.0 18 7802.0 19 4440.0 20 4153.0 21 4427.0 22 4692.0 23 5227.0 24 5763.0 25 6433.0 26 7490.0 27 8418.0 28 9805.0 29 11653.0 30 13531.0 31 16036.0 32 20303.0 33 25178.0 34 34767.0 35 58092.0 36 72331.0 37 113512.0 38 123881.0 39 19765.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.173446654014356 22.673520270390522 16.578084323918528 18.574948751676594 2 14.201280020687204 24.028024695348613 41.26709118531209 20.5036040986521 3 16.91665710448528 31.524976687132426 32.036809280541476 19.521556927840816 4 10.593077182960165 20.030519494165247 40.97242091684569 28.403982406028895 5 10.27605105529913 41.38890460677881 34.73021364654148 13.60483069138058 6 24.36102417013085 41.4338640232302 19.060585212389007 15.144526594249944 7 23.624328881825054 37.706019550455814 23.252669935844008 15.416981631875126 8 24.560726164840972 34.61317894199502 21.597879272995137 19.228215620168875 9 27.198621030576092 17.582183249786354 22.926174409907386 32.29302130973017 10-11 22.368646340123924 29.876900363758534 27.87695528289818 19.87749801321936 12-13 25.132540686861578 28.667874237159204 28.436750736292282 17.762834339686943 14-15 22.538380522853856 28.933438222865487 29.332589199178116 19.19559205510254 16-17 17.50801430866999 32.642097528282235 30.243618833827245 19.60626932922053 18-19 17.281232356796757 30.017615943357594 35.31394906480422 17.387202635041433 20-21 19.086807056380756 28.887291000973548 34.995657425398605 17.03024451724709 22-23 18.726584800490585 28.935349205105233 34.65057675014604 17.68748924425815 24-25 18.180554545381078 29.719494751133418 35.17855844061386 16.92139226287164 26-27 17.82548765175742 29.545531670095166 35.37085126358808 17.25812941455934 28-29 17.33549867494021 29.20948872083253 36.10246590394933 17.35254670027794 30-31 17.629238018989945 30.05623628124765 35.33764793228056 16.97687776748184 32-33 17.143374234764575 30.60101430809467 34.80077014375963 17.454841313381124 34-35 17.57613581449112 29.96751635716951 34.98544185606015 17.470905972279223 36-37 18.05778315553506 29.57899778667287 34.51169432582672 17.851524731965355 38-39 17.66387698390194 30.339784981082545 34.74394601032156 17.252392024693957 40-41 17.040590154797357 30.31129596204759 35.45376332239134 17.194350560763713 42-43 17.07732298751663 30.62898157556546 34.259578874697965 18.034116562219943 44-45 17.247534989854724 30.634679337996484 33.80532092130767 18.312464750841116 46-47 17.71653989961281 30.77732811894764 32.96959049352443 18.536541487915127 48-49 17.595986692546774 30.936505942563997 32.77223750805376 18.695269856835466 50-51 17.785073093171405 31.08082591758491 32.56367845973939 18.57042252950429 52-53 17.619694430306705 31.542468870857416 32.4285269699455 18.40930972889037 54-55 17.67836537838223 31.187687181548274 32.35704515694079 18.776902283128702 56-57 17.893020475410555 31.67605467237561 31.557631338928875 18.87329351328496 58-59 17.706288269025173 30.794618848608547 32.45383419185538 19.045258690510902 60-61 17.853962600178093 31.623411317088966 32.042418845624546 18.480207237108395 62-63 18.16435722841476 31.273937898829914 32.39110737955884 18.170597493196492 64-65 18.070557039495863 30.84463577189388 32.594703845854625 18.490103342755635 66-67 17.840753810467824 31.25969535708902 31.778726218514908 19.120824613928246 68-69 17.73096152100328 31.17834632393946 32.258095892720505 18.832596262336754 70-71 18.064840330685687 30.99610958015886 31.989463446263578 18.949586642891877 72-73 17.852971902294644 30.61903453596926 31.937808834458654 19.590184727277443 74-75 17.386148949569105 31.482533612897107 31.719357738159403 19.411959699374385 76-77 17.569459750294953 30.947835500641762 32.36198804631083 19.12071670275246 78-79 17.148021606486157 30.475046161300018 32.59372627625158 19.78320595596224 80-81 17.392847218115044 31.506883720101207 31.472856032906392 19.627413028877353 82-83 17.560402091810857 30.638190295151464 31.659382277299443 20.142025335738232 84-85 17.002953533503764 30.144597545609606 31.849057826863785 21.00339109402284 86-87 17.260527721033725 30.545442464502443 31.834155930764062 20.359873883699766 88-89 17.700113287726516 29.653427075280426 32.03214518980961 20.614314447183446 90-91 17.276482068639083 29.60409007501929 32.40304875281241 20.716379103529214 92-93 17.52755384732599 30.01938779314716 31.88765130502867 20.565407054498177 94-95 17.202191975347166 29.148230671282317 32.273765109140825 21.375812244229692 96-97 17.51626273907807 28.920280563656082 31.93056908644985 21.632887610816 98-99 17.459609901429843 28.89478555601122 31.76881642868374 21.876788113875197 100-101 17.64333125075463 28.037911035803337 32.71163902185249 21.60711869158954 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4818.0 1 3088.0 2 1599.0 3 1804.0 4 1482.0 5 1546.0 6 2918.5 7 6050.0 8 8606.5 9 8372.0 10 6140.5 11 4195.5 12 3746.0 13 3555.5 14 3286.0 15 2930.0 16 2629.5 17 2466.5 18 2334.5 19 2349.5 20 2335.0 21 2370.0 22 2627.0 23 2782.5 24 2990.5 25 3444.5 26 4194.0 27 5018.0 28 5397.0 29 6353.5 30 8305.5 31 10118.0 32 12957.0 33 16862.5 34 20221.5 35 22370.0 36 26490.0 37 31057.5 38 30876.5 39 29330.0 40 27516.5 41 24950.5 42 22134.0 43 21535.0 44 21618.0 45 20143.5 46 18217.0 47 17038.0 48 22074.0 49 21243.5 50 14285.5 51 10728.0 52 7910.5 53 6778.0 54 6107.5 55 5576.0 56 5090.5 57 4513.5 58 3921.5 59 3603.0 60 3366.5 61 3230.5 62 2872.5 63 2024.5 64 1297.0 65 1001.0 66 816.5 67 620.5 68 488.5 69 352.0 70 243.0 71 181.5 72 167.0 73 161.0 74 120.0 75 90.5 76 67.0 77 47.0 78 37.0 79 22.5 80 11.5 81 11.0 82 11.0 83 5.0 84 3.5 85 3.5 86 3.5 87 2.5 88 3.5 89 4.5 90 2.0 91 1.0 92 1.0 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2897405080138417 2 0.18149113113339196 3 0.17874859848515404 4 0.20068885967105743 5 0.21294959386317988 6 0.10824937688044978 7 0.12083276197236495 8 0.13422277431376187 9 0.13744928331168885 10-11 0.12470457276987731 12-13 0.11542835940083727 14-15 0.12922168536697506 16-17 0.10647479693158994 18-19 0.1328515079896429 20-21 0.1601155090221258 22-23 0.1628580416703637 24-25 0.18713752187976415 26-27 0.16648786429303153 28-29 0.16535858614375712 30-31 0.15463044372564994 32-33 0.16447129616932718 34-35 0.34999556355012784 36-37 0.47115097642228554 38-39 0.43719196921910414 40-41 0.43090027667314657 42-43 0.45235656150936093 44-45 0.45638969775676963 46-47 0.4604228340041783 48-49 0.4706670000725965 50-51 0.4643753075266389 52-53 0.470908988247441 54-55 0.4544537923580135 56-57 0.41751026433174965 58-59 0.4073467609882797 60-61 0.3573358715204117 62-63 0.4684891064989958 64-65 0.4660692247505505 66-67 0.4752647753946424 68-69 0.48542827873811234 70-71 0.478329958942673 72-73 0.4724415800214563 74-75 0.4761520653690723 76-77 0.4544537923580135 78-79 0.44130576819146106 80-81 0.43888588644301585 82-83 0.4504206561106047 84-85 0.45292120058399815 86-87 0.454211804183169 88-89 0.46010018310438566 90-91 0.4770393553435022 92-93 0.481072491590911 94-95 0.4805078525162737 96-97 0.4781686334927766 98-99 0.4749421244948497 100-101 0.45840626588047395 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 619865.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 32.561874357238594 #Duplication Level Percentage of deduplicated Percentage of total 1 74.59673731178756 24.290095878063585 2 11.409799452679147 7.430489124388559 3 4.385334514991397 4.2838413447483505 4 2.288739736885613 2.9810262299555452 5 1.3664608977444597 2.224726403321727 6 0.8816042955125019 1.7224012981967955 7 0.6689731788003573 1.5248114417521819 8 0.48208554271577975 1.2558087097081918 9 0.36698217379115855 1.0754664690900602 >10 2.8496412195436345 19.088933743127836 >50 0.42724646166945834 9.603605098099061 >100 0.2605161351641766 14.984021784303891 >500 0.008931981777057485 1.819217137752494 >1k 0.004962212098365269 2.711294393062159 >5k 0.0014886636295095806 3.236749063278973 >10k+ 4.962212098365269E-4 1.7675118811505932 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10939 1.7647390964161551 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8028 1.295120711767885 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6690 1.0792672598065707 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5314 0.8572834407491953 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2861 0.4615521121534527 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2101 0.338944770232228 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1994 0.3216829470933187 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1692 0.2729626612246215 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1588 0.25618481443540125 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1471 0.2373097367975285 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1445 0.23311527510022342 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1246 0.20101151057085012 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1236 0.19939825607188663 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1146 0.18487896558121525 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 851 0.1372879578617925 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 840 0.13551337791293264 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 775 0.12502722366967 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 769 0.12405927097029193 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 668 0.10776540053076074 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 653 0.1053455187823155 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 639 0.10308696248376661 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 1.613254498963484E-4 0.0 22-23 0.0 0.0 0.0 0.001209940874222613 0.0 24-25 0.0 0.0 0.0 0.00403313624740871 0.0 26-27 0.0 0.0 0.0 0.009114887919143683 0.0 28-29 0.0 0.0 0.0 0.017181160413961106 0.0 30-31 0.0 0.0 0.0 0.042993232397376846 0.0 32-33 0.0 0.0 0.0 0.08259863034693038 0.0 34-35 0.0 0.0 0.0 0.14132109410920118 0.0 36-37 0.0 0.0 0.0 0.2210158663579973 0.0 38-39 0.0 0.0 0.0 0.3313624740870996 0.0 40-41 0.0 0.0 0.0 0.48058851524122187 0.0 42-43 0.0 0.0 0.0 0.6123107450815903 0.0 44-45 0.0 0.0 0.0 0.7667798633573439 0.0 46-47 0.0 0.0 0.0 0.9609350423075992 0.0 48-49 0.0 0.0 0.0 1.173481322546038 0.0 50-51 0.0 0.0 0.0 1.3861082655094255 0.0 52-53 0.0 0.0 0.0 1.6061561791680448 0.0 54-55 0.0 0.0 0.0 1.8426592887160913 0.0 56-57 0.0 0.0 0.0 2.1169932162648317 0.0 58-59 0.0 0.0 0.0 2.4254474764666503 0.0 60-61 0.0 0.0 0.0 2.771651891944214 0.0 62-63 0.0 0.0 0.0 3.1008364724577127 0.0 64-65 0.0 0.0 0.0 3.438006662741081 0.0 66-67 0.0 0.0 0.0 3.8338993167867197 0.0 68-69 0.0 0.0 0.0 4.283997321997532 0.0 70-71 0.0 0.0 0.0 4.800158098940899 0.0 72-73 0.0 0.0 0.0 5.314140982310665 0.0 74-75 0.0 0.0 0.0 5.846918280593355 0.0 76-77 0.0 0.0 0.0 6.390262395844257 0.0 78-79 0.0 0.0 0.0 7.016931105966622 0.0 80-81 0.0 0.0 0.0 7.702564268026102 0.0 82-83 0.0 8.06627249481742E-5 0.0 8.389891347309494 0.0 84-85 0.0 1.613254498963484E-4 0.0 9.118033765416662 0.0 86-87 0.0 1.613254498963484E-4 0.0 9.873278859106417 0.0 88-89 0.0 1.613254498963484E-4 0.0 10.626668710122367 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATACAGG 105 4.7539288E-8 36.101124 6 TAGACCC 70 2.0723887E-4 33.87218 5 GCTTCGG 70 2.0862142E-4 33.833866 9 TGGGAGT 255 0.0 29.730341 6 CATATAG 160 1.9354047E-9 29.63816 3 GTATGAC 65 0.0058896863 29.182188 3 CATGGGT 360 0.0 28.97953 4 CATTCCG 85 6.509331E-4 27.863184 9 GTCCTGG 205 3.274181E-11 27.769897 1 ATGACCG 70 0.00845696 27.097744 5 GGTACTC 105 7.017868E-5 27.075844 8 AGATAGT 70 0.008490383 27.075842 6 GATAGTC 70 0.008490383 27.075842 7 GTACAGG 125 7.456212E-6 26.566538 1 GGGATAG 125 7.526809E-6 26.534328 7 GGGATGC 180 6.864866E-9 26.323736 7 GTACATG 6395 0.0 25.889984 1 ATGGGAT 1835 0.0 25.584112 5 GGATGAC 260 1.8189894E-12 25.513777 8 GGTATCA 7805 0.0 25.346033 1 >>END_MODULE