FastQCFastQC Report
Fri 27 May 2016
SRR939299_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939299_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences619865
Sequences flagged as poor quality0
Sequence length101
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110631.7847434522033023No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88241.4235357698853783No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73711.189129891185984No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA51560.8317940196655724No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27210.43896654916796396No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA21440.345881764577771No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21430.3457204391278746No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17840.28780460261508556No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA17430.28119025916933527No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14040.22650093165447316No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT13370.2156921265114178No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13060.210691037564631No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT12760.20585127406774054No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11350.18310438563235543No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG8620.13906253781065234No Hit
TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC7650.12341396917070653No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT7550.12180071467174304No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT7530.12147806377195035No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG7130.11502504577609642No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA6760.10905600412993152No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC6410.10340961338355932No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT6390.10308696248376661No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACTC1101.6298145E-938.833088
AGGACCG500.00162149537.9701235
GTATGGT1404.4201443E-1034.014391
CGCAGAA753.089589E-431.6443219
GTGGTAC1601.9172148E-929.6641586
TACCTGG2750.029.340552
CTGACCG650.00586451929.2077874
TGACCGT650.00586451929.2077875
CATATAG1802.1282176E-1029.0049553
GACAGAC1153.8845083E-628.890317
ACGCCGA700.00842088627.1215176
CTCCGAC705.8467594E-727.12151794-95
ACCGTGC700.00842088627.1215177
ACGGAGG1754.993126E-927.1215158
TGGTACT1806.7429937E-926.3681417
ACACGGA1859.025825E-925.6554876
TATAGGT1901.198714E-824.9803433
TGGGAGT1901.198714E-824.9803436
GTAGATA1951.5799742E-824.339826
CTACACG2151.6734703E-924.2832164
Produced by FastQC (version 0.11.5)