##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939299_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 619865 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.21240108733353 33.0 31.0 34.0 30.0 34.0 2 32.402339219023496 34.0 31.0 34.0 31.0 34.0 3 32.446135852161355 34.0 31.0 34.0 30.0 34.0 4 36.032703895202985 37.0 35.0 37.0 35.0 37.0 5 35.99699612012293 37.0 35.0 37.0 35.0 37.0 6 36.0555620981988 37.0 35.0 37.0 35.0 37.0 7 36.00533341937357 37.0 35.0 37.0 35.0 37.0 8 35.98893146088261 37.0 35.0 37.0 35.0 37.0 9 37.64104119445363 39.0 37.0 39.0 35.0 39.0 10-11 37.568528631234216 39.0 37.0 39.0 35.0 39.0 12-13 37.49885539593299 39.0 37.0 39.0 35.0 39.0 14-15 38.62857799682189 40.0 38.0 41.0 34.0 41.0 16-17 38.64437337162124 40.0 38.0 41.0 34.5 41.0 18-19 38.847665217426375 40.0 38.0 41.0 35.0 41.0 20-21 38.877647552289616 40.0 38.0 41.0 35.0 41.0 22-23 38.77668685923548 40.0 38.0 41.0 34.5 41.0 24-25 38.695257838400295 40.0 38.0 41.0 34.0 41.0 26-27 38.48661079428585 40.0 38.0 41.0 34.0 41.0 28-29 38.27822348414574 40.0 38.0 41.0 34.0 41.0 30-31 37.9471699482952 40.0 37.0 41.0 33.5 41.0 32-33 37.66250554556234 40.0 35.5 41.0 33.0 41.0 34-35 37.494319730909154 40.0 35.0 41.0 33.0 41.0 36-37 37.26681051519283 40.0 35.0 41.0 33.0 41.0 38-39 37.07585280665951 40.0 35.0 41.0 32.5 41.0 40-41 36.84624636009454 39.5 35.0 41.0 31.5 41.0 42-43 36.57517201326095 39.0 35.0 41.0 31.0 41.0 44-45 36.17914465246465 39.0 35.0 41.0 30.0 41.0 46-47 35.64672549668073 39.0 35.0 41.0 28.0 41.0 48-49 35.18291724811047 39.0 35.0 41.0 25.5 41.0 50-51 34.93500278286401 39.0 35.0 40.5 24.0 41.0 52-53 34.69234188089342 38.0 35.0 40.0 24.0 41.0 54-55 34.46919490534229 38.0 34.5 40.0 23.0 41.0 56-57 34.195866841973654 38.0 34.0 40.0 22.5 41.0 58-59 33.91362716075274 38.0 34.0 40.0 22.0 41.0 60-61 33.592829890379356 37.5 33.5 40.0 20.5 41.0 62-63 33.26398489993789 37.0 33.0 40.0 19.0 41.0 64-65 32.98061352068596 36.5 33.0 39.5 16.5 41.0 66-67 32.57718454824841 36.0 33.0 39.0 12.0 40.5 68-69 32.11027239802215 35.5 32.0 39.0 7.5 40.0 70-71 31.644475813281925 35.0 31.5 38.0 3.5 40.0 72-73 31.12721882990651 35.0 31.0 37.5 2.0 39.5 74-75 30.588896775910882 35.0 31.0 37.0 2.0 39.0 76-77 28.605030934155018 32.5 28.0 35.0 2.0 37.5 78-79 29.54415477563663 34.0 29.5 36.0 2.0 38.0 80-81 29.517575601138958 34.0 30.0 36.0 2.0 37.0 82-83 29.135155235414164 34.0 29.5 35.0 2.0 37.0 84-85 28.663975220410897 34.0 29.0 35.0 2.0 36.5 86-87 28.06912069563534 34.0 28.5 35.0 2.0 36.0 88-89 27.453485839658637 33.5 26.5 35.0 2.0 35.5 90-91 26.937951005460867 33.0 26.0 35.0 2.0 35.0 92-93 26.570437111306497 33.0 25.0 35.0 2.0 35.0 94-95 26.079818992845215 33.0 24.5 35.0 2.0 35.0 96-97 25.388140159550872 32.5 21.5 34.5 2.0 35.0 98-99 24.51615835706162 32.0 9.0 34.0 2.0 35.0 100-101 20.49788663660636 27.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2848067117980122 1101 2 0.3289718571375744 1101 3 0.2951827630663928 1101 4 0.23322517075234117 1101 5 0.18182442895336237 1101 6 0.19332993102268148 1101 7 0.22485866435545177 1101 8 0.24679111284552846 1101 9 0.49923221948686347 1101 10-11 0.35611464332099985 1101 12-13 0.2484842327387753 1101 14-15 0.4715393871051745 1101 16-17 0.45378699524563615 1101 18-19 0.5341602269119079 1101 20-21 0.452114502586916 1101 22-23 0.5702229651124426 1101 24-25 0.4408305620464148 1101 26-27 0.4361422140824587 1101 28-29 0.6489992202517172 1101 30-31 0.6210742734883112 1101 32-33 0.7909685222659917 1101 34-35 0.8718310177478941 1101 36-37 0.6713703337721384 1101 38-39 0.9418632265030382 1101 40-41 0.8435708383153298 1101 42-43 0.7823162584788079 1101 44-45 0.986674279403168 1101 46-47 0.8142595077460015 1101 48-49 0.9888007592854109 1101 50-51 0.8726935279460974 1101 52-53 0.8812011750514444 1101 54-55 1.0402583906511254 1101 56-57 1.1168797747352173 1101 58-59 1.2088692705337465 1101 60-61 1.1421272704920966 1101 62-63 1.032904600543823 1101 64-65 1.1338180583443176 1101 66-67 1.0309867199254867 1101 68-69 0.9948861378651728 1101 70-71 1.0112048852988167 1101 72-73 0.5654131867365457 1101 74-75 0.8475069934946404 1101 76-77 0.6384624380689097 1101 78-79 0.7294467397902231 1101 80-81 0.9205853715695156 1101 82-83 0.841674952171175 1101 84-85 0.7354059772646231 1101 86-87 0.296842380370812 1101 88-89 0.6330099933301518 1101 90-91 1.114259595025061 1101 92-93 1.1971209668926157 1101 94-95 0.8468057205243262 1101 96-97 0.5824445655954769 1101 98-99 0.3449084067810233 1101 100-101 0.7037083106814741 1102 1 0.07503825395616559 1102 2 0.16367696853993152 1102 3 0.1606611379025651 1102 4 0.2322640523600299 1102 5 0.18591355084065242 1102 6 0.2205208440851507 1102 7 0.2341203506813585 1102 8 0.21893615434836278 1102 9 0.25250439074068964 1102 10-11 0.26696654371418305 1102 12-13 0.18669305755310717 1102 14-15 0.2697219995997102 1102 16-17 0.2726074408550758 1102 18-19 0.2104206026218236 1102 20-21 0.19539978000063485 1102 22-23 0.3443514054794221 1102 24-25 0.3628401732303317 1102 26-27 0.33937506685659713 1102 28-29 0.3652508585217049 1102 30-31 0.24725164351889362 1102 32-33 0.41026952707158415 1102 34-35 0.39223293998468023 1102 36-37 0.4826940559215487 1102 38-39 0.4903385432352394 1102 40-41 0.6205389466595861 1102 42-43 0.579170780103972 1102 44-45 0.2870762016399411 1102 46-47 0.4902752351378794 1102 48-49 0.5107317335099708 1102 50-51 0.5066297446346013 1102 52-53 0.3317822148068075 1102 54-55 0.0709530171255679 1102 56-57 0.15089462838309942 1102 58-59 0.2741816340112493 1102 60-61 0.2999603853894328 1102 62-63 0.3285883052183536 1102 64-65 0.23025318285284158 1102 66-67 0.3505935351286311 1102 68-69 0.28665546945101283 1102 70-71 0.22535953798557884 1102 72-73 0.5945088617037761 1102 74-75 0.3604209297113279 1102 76-77 0.40823089591076567 1102 78-79 0.5693681028308539 1102 80-81 0.8170991330374058 1102 82-83 0.676939259729064 1102 84-85 0.9104168990645363 1102 86-87 0.4323163864870203 1102 88-89 0.5593883244791265 1102 90-91 0.7467978213247548 1102 92-93 0.6547880704312767 1102 94-95 0.9869542570031378 1102 96-97 0.6813261732844254 1102 98-99 0.3834754666324862 1102 100-101 0.6389988305591494 1103 1 0.1826513233268372 1103 2 0.13784403758869956 1103 3 0.1610474247205218 1103 4 0.11317504543905699 1103 5 0.1160600179257969 1103 6 0.1851595049575181 1103 7 0.11232733603465306 1103 8 0.038620686780362234 1103 9 0.06449537738160416 1103 10-11 -0.0472938779822627 1103 12-13 0.1427448843678505 1103 14-15 0.20184013898487052 1103 16-17 0.16664413810277523 1103 18-19 0.124536166761537 1103 20-21 0.22023480333879775 1103 22-23 0.24197735107735951 1103 24-25 0.11471527382585833 1103 26-27 0.10844797849348708 1103 28-29 0.17606688942465354 1103 30-31 0.39142515068130024 1103 32-33 0.41472792076223897 1103 34-35 0.19401146887571485 1103 36-37 0.3273101833962002 1103 38-39 0.26281560745542265 1103 40-41 0.3492851240296204 1103 42-43 0.24572477978206564 1103 44-45 0.45552139719764995 1103 46-47 0.5760389563675545 1103 48-49 0.0899285788342894 1103 50-51 0.08956069586589166 1103 52-53 0.23190292943741042 1103 54-55 0.32098520769373096 1103 56-57 0.12404769453471687 1103 58-59 0.38468380687459813 1103 60-61 0.3369393005672876 1103 62-63 0.2047592371353062 1103 64-65 -0.04227216721959337 1103 66-67 0.04685138157962143 1103 68-69 0.33024954638647586 1103 70-71 0.07060338153941714 1103 72-73 0.28107734713754695 1103 74-75 -0.0128689463549847 1103 76-77 0.20412659846991232 1103 78-79 0.20633897036666227 1103 80-81 0.46725203823618244 1103 82-83 0.3192939997902222 1103 84-85 0.2939272805227695 1103 86-87 0.32315816984449697 1103 88-89 0.3601528504730105 1103 90-91 0.3237332792355865 1103 92-93 0.28960216990013166 1103 94-95 -0.09905894112980818 1103 96-97 0.16863504178595434 1103 98-99 -0.01729710700343645 1103 100-101 0.13335743348849505 1104 1 -4.7790293597671507E-4 1104 2 0.14839839862450788 1104 3 0.07831394804482272 1104 4 0.06360663061870042 1104 5 0.07495815135884243 1104 6 -0.03907470401072999 1104 7 0.12644032721598109 1104 8 0.1351680053432034 1104 9 0.2939273053364815 1104 10-11 0.1984541510601474 1104 12-13 0.1467350996930179 1104 14-15 0.08311682092061545 1104 16-17 0.14968143828040326 1104 18-19 0.2561275786838806 1104 20-21 0.22106992845175455 1104 22-23 0.24173273420658603 1104 24-25 0.2433899801298054 1104 26-27 0.3108271502238793 1104 28-29 0.26379241994722946 1104 30-31 0.0983507684557452 1104 32-33 0.26794221760558656 1104 34-35 0.3852878391976091 1104 36-37 0.36111405364018623 1104 38-39 0.3486060159811828 1104 40-41 0.4303390020639597 1104 42-43 0.3113444239190528 1104 44-45 0.2760458433040327 1104 46-47 0.44909838112839395 1104 48-49 0.2115530218245354 1104 50-51 0.0827129815534704 1104 52-53 0.05425031655529722 1104 54-55 0.14351644698200516 1104 56-57 0.0660314282918435 1104 58-59 0.01984787156563783 1104 60-61 0.03757343474820374 1104 62-63 0.23544541082886639 1104 64-65 0.1684691621250849 1104 66-67 0.42033460117389865 1104 68-69 0.3650053969364748 1104 70-71 -0.011251344084591608 1104 72-73 -0.12867460823935417 1104 74-75 -0.19588131742475667 1104 76-77 -0.016608186983955164 1104 78-79 0.18981720413936998 1104 80-81 0.13681982982884122 1104 82-83 0.08504296367418007 1104 84-85 -0.023521536902677553 1104 86-87 0.05977902927548939 1104 88-89 0.214830174333283 1104 90-91 0.035562140910197826 1104 92-93 0.48222627011514874 1104 94-95 0.09675920102842994 1104 96-97 0.01378009803225666 1104 98-99 0.017796844571773107 1104 100-101 -0.31513123934812626 1105 1 0.15721727187696644 1105 2 0.10145643646153246 1105 3 0.076964159562813 1105 4 0.0710243318675623 1105 5 0.03643089330076066 1105 6 0.1380819665519155 1105 7 0.14131560982794156 1105 8 -0.027800547652866214 1105 9 0.08237557009135799 1105 10-11 0.14535224509746314 1105 12-13 0.1857455206736347 1105 14-15 0.17434718379897873 1105 16-17 0.17674776504578915 1105 18-19 0.17011384151837206 1105 20-21 0.25866767307790894 1105 22-23 0.0330554348879204 1105 24-25 0.13087744090157827 1105 26-27 0.2980716490688877 1105 28-29 0.15299625948191675 1105 30-31 0.20884629421369993 1105 32-33 0.09502971150853057 1105 34-35 0.5339578373876677 1105 36-37 0.5659521250378674 1105 38-39 0.4647154347438516 1105 40-41 0.46914695477228463 1105 42-43 0.35428763770407556 1105 44-45 0.20790299887021746 1105 46-47 0.2580542277065234 1105 48-49 0.4194940728802834 1105 50-51 0.706436434435119 1105 52-53 0.8735673235834014 1105 54-55 1.0138631278828072 1105 56-57 0.8785427404396344 1105 58-59 0.8961873065564419 1105 60-61 0.7938568535170134 1105 62-63 1.0262527377261605 1105 64-65 0.8304575846211506 1105 66-67 0.7668268383562733 1105 68-69 0.9225002508678841 1105 70-71 0.8590953368162104 1105 72-73 0.8238143710444668 1105 74-75 0.7457848123942199 1105 76-77 1.0322694945702686 1105 78-79 0.958797837742356 1105 80-81 1.0128478931584617 1105 82-83 0.9537499583394471 1105 84-85 0.9193931472621557 1105 86-87 1.2011601696626961 1105 88-89 1.2022081181759496 1105 90-91 1.0985082996882731 1105 92-93 0.8167360668185957 1105 94-95 1.0618809734234134 1105 96-97 0.8870288241693736 1105 98-99 1.0149454164035276 1105 100-101 1.1553145282980566 1106 1 0.11768874825509101 1106 2 0.153100982649498 1106 3 0.16184621765564344 1106 4 0.16108191209980305 1106 5 0.11804220780183527 1106 6 0.0860529696479091 1106 7 0.12563759312763523 1106 8 0.21371313966637473 1106 9 0.26181794180277507 1106 10-11 0.2795692325765984 1106 12-13 0.1501721794182913 1106 14-15 0.3220155775813609 1106 16-17 0.10922423928321479 1106 18-19 0.1999451783292372 1106 20-21 0.22834994786114748 1106 22-23 0.3293460679792233 1106 24-25 0.20066835213714995 1106 26-27 0.004591583712262093 1106 28-29 0.2711143732269079 1106 30-31 0.37757792722636907 1106 32-33 0.3981603635893691 1106 34-35 0.5227665352938686 1106 36-37 0.47252186352400116 1106 38-39 0.7683805315700951 1106 40-41 0.36553020553608917 1106 42-43 0.3603456489871206 1106 44-45 0.5098526300014115 1106 46-47 0.5774250100346094 1106 48-49 0.6217486433294255 1106 50-51 0.5404571190486465 1106 52-53 0.6240791964117705 1106 54-55 0.49890450002235553 1106 56-57 0.9717356327614013 1106 58-59 1.0414557958690054 1106 60-61 0.9206513209217135 1106 62-63 1.0480848783885222 1106 64-65 0.8287494001896576 1106 66-67 1.094614761361285 1106 68-69 1.0288634927194167 1106 70-71 0.8441367089557659 1106 72-73 0.8285064172833962 1106 74-75 0.9295129358378489 1106 76-77 0.7902009749676502 1106 78-79 1.0533494138446677 1106 80-81 0.9562346127162265 1106 82-83 0.966202375282073 1106 84-85 1.1085851308822257 1106 86-87 1.3171346794152186 1106 88-89 1.47176049521725 1106 90-91 1.5700662279649151 1106 92-93 1.2977232314071898 1106 94-95 1.5774890899935308 1106 96-97 1.6548351542086621 1106 98-99 1.8775639917806224 1106 100-101 2.2062265793717515 1107 1 0.10464208374970241 1107 2 0.1611158682153473 1107 3 0.05289335618893887 1107 4 -0.018732672158655816 1107 5 0.03687174028500095 1107 6 0.09260218273434617 1107 7 -0.016298347722397466 1107 8 0.041055287012767394 1107 9 0.05610890230862253 1107 10-11 -0.01739562669977346 1107 12-13 0.09149215642521824 1107 14-15 0.12250023024694912 1107 16-17 0.09935819303008486 1107 18-19 0.13648809828662678 1107 20-21 0.07759953989747714 1107 22-23 0.24838128498320344 1107 24-25 0.3156732422525792 1107 26-27 0.4047705701098465 1107 28-29 0.39056395600062643 1107 30-31 0.19111196869563685 1107 32-33 0.2071066867401825 1107 34-35 0.36312265556618684 1107 36-37 0.3334151064469566 1107 38-39 0.22226633443442267 1107 40-41 0.16118603948464738 1107 42-43 0.18658581767567028 1107 44-45 0.18623732271418447 1107 46-47 -0.006719029064512938 1107 48-49 -0.11818439341863751 1107 50-51 -0.0808967482289944 1107 52-53 0.19899331290821465 1107 54-55 -0.09354728670867019 1107 56-57 -0.1616678058479053 1107 58-59 -0.07764507672373355 1107 60-61 0.08298272091135317 1107 62-63 -0.01807470555664281 1107 64-65 -0.16025533191646701 1107 66-67 -0.007910118292834056 1107 68-69 -0.029965004530403405 1107 70-71 -0.16026984352647844 1107 72-73 0.1321798610961089 1107 74-75 -0.07789034476214951 1107 76-77 -0.1029561325903714 1107 78-79 0.1863264997717593 1107 80-81 0.37958375606062944 1107 82-83 0.28808775315586743 1107 84-85 0.3998493959891398 1107 86-87 0.5024428111731822 1107 88-89 0.7021830066388723 1107 90-91 0.7262897511909223 1107 92-93 0.8548177127961445 1107 94-95 0.9603606093619312 1107 96-97 1.0317745140530512 1107 98-99 0.9046653111582827 1107 100-101 0.8530567220977368 1108 1 0.04291090645393325 1108 2 0.1154384939490356 1108 3 0.03573806982859651 1108 4 0.13728547826092097 1108 5 0.002657389096668794 1108 6 0.053404569028352 1108 7 0.12144024369565187 1108 8 0.16015728690135234 1108 9 0.28357913693722736 1108 10-11 0.21217375662148896 1108 12-13 0.15197878416435628 1108 14-15 0.13157670610800665 1108 16-17 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-0.8394548704882929 2316 88-89 -0.7931547096644458 2316 90-91 -0.8399242537721925 2316 92-93 -0.856965925596338 2316 94-95 -0.8435538769229005 2316 96-97 -1.1632378104477255 2316 98-99 -1.1997577689676255 2316 100-101 -1.2125889401776284 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 8.0 5 42.0 6 85.0 7 206.0 8 304.0 9 602.0 10 983.0 11 1506.0 12 2127.0 13 2923.0 14 3639.0 15 5012.0 16 6853.0 17 10086.0 18 8902.0 19 5074.0 20 4164.0 21 4517.0 22 4943.0 23 5388.0 24 6177.0 25 6861.0 26 7774.0 27 9024.0 28 10804.0 29 12969.0 30 15320.0 31 18208.0 32 22056.0 33 27690.0 34 38643.0 35 61535.0 36 76697.0 37 125839.0 38 105851.0 39 7053.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.344300967056654 23.145126451919793 17.124037918587323 18.386534662436233 2 14.456212239761884 24.471457494777088 40.712735837642064 20.359594427818962 3 16.86286530131561 31.43006945060618 32.44932364305131 19.2577416050269 4 10.717978914763698 20.694344736353884 40.26667096867867 28.321005380203758 5 10.078323505924677 41.59615400127447 34.89646939252901 13.429053100271835 6 24.27431779500375 41.82943060182459 18.637606575625338 15.258645027546319 7 23.562065933711374 37.74450888499915 22.893210618441113 15.80021456284836 8 24.729094238261556 35.100546086647896 21.161220588353917 19.009139086736628 9 26.797286505932743 17.68223726133916 22.613472288320843 32.90700394440725 10-11 22.50369031966638 30.02161761028611 27.602381163640473 19.87231090640704 12-13 25.239769949908446 28.83635952989764 28.23542222903374 17.68844829116017 14-15 22.31633017695253 29.158408693639082 29.202612544375263 19.322648585033125 16-17 17.424712959840996 32.6937006845083 30.190236198115382 19.691350157535318 18-19 17.241657457672236 29.827865744960597 35.45360683374606 17.476869963621112 20-21 18.922749308317133 28.751260355077314 35.352697764835895 16.97329257176966 22-23 18.588240987957054 28.738999620885192 35.109580311842095 17.563179079315656 24-25 17.997628515886525 29.614512837472674 35.52047623272809 16.867382413912708 26-27 17.766529808910004 29.341308188073214 35.89047615206537 17.001685850951418 28-29 17.172690827841546 29.09843272325426 36.54255362054641 17.186322828357785 30-31 17.57810168343107 29.77277310382099 35.79222895307849 16.856896259669444 32-33 17.05282601856856 30.326926024214952 35.25848370209642 17.361764255120065 34-35 17.522110045268725 29.594791676749686 35.57676485107783 17.306333426903755 36-37 17.954416565761843 29.384640578501266 34.97435094872045 17.686591907016442 38-39 17.557415702512007 29.998459288513956 35.20420880431915 17.239916204654886 40-41 16.989130215580698 29.986407727831892 35.8287454373122 17.195716619275213 42-43 17.000880841219498 30.158486889017233 34.837592883577315 18.003039386185957 44-45 17.13404860987314 30.285623941786245 34.33464520245509 18.24568224588552 46-47 17.726763085510555 30.369596605712534 33.422035443201345 18.48160486557557 48-49 17.662142813930508 30.589687229022566 33.07475448184073 18.673415475206195 50-51 17.786776152871997 30.734514773378073 32.86489800198431 18.613811071765625 52-53 17.613673945133215 31.274551716906103 32.704137191162594 18.40763714679809 54-55 17.531075314786285 31.103708065465867 32.50885273406306 18.856363885684786 56-57 17.9811343888439 31.56327739579065 31.487130923534103 18.968457291831356 58-59 17.596681116469064 30.697333374203634 32.62982639264205 19.076159116685247 60-61 17.853040535090294 31.58239467749065 32.046533238257794 18.51803154916126 62-63 18.114786430063134 31.231391832451273 32.46054927317738 18.19327246430821 64-65 17.988594290692326 30.825986303469303 32.70115267033951 18.48426673549886 66-67 17.789230166117886 31.198768756221153 31.812938707226294 19.19906237043466 68-69 17.674271157839037 31.10939206553812 32.25950346291782 18.956833313705022 70-71 17.94390714107104 31.031595589362198 32.02148854992619 19.003008719640565 72-73 17.812023585780775 30.660305066425753 31.750703782275174 19.776967565518298 74-75 17.417582860784204 31.49677752413832 31.42877884700701 19.656860768070466 76-77 17.37015848985804 30.913285878568203 32.40138256077817 19.315173070795588 78-79 16.989021803134555 30.522855783114068 32.49957652069402 19.98854589305736 80-81 17.177127277713698 31.548562993555045 31.429585474256495 19.844724254474766 82-83 17.31836770909795 30.79509247981415 31.51145814007889 20.37508167100901 84-85 16.86423656763973 30.21391754656256 31.61422245166286 21.30762343413485 86-87 17.002587943654692 30.5697109658561 31.76404090646663 20.66366018402258 88-89 17.45627329635063 29.813418697962714 31.831518201513635 20.89878980417302 90-91 16.94787353900082 29.736322556589833 32.19313861162717 21.122665292782173 92-93 17.094044671017077 30.241584861219785 31.73352262186121 20.93084784590193 94-95 16.703879070442756 29.597734990683456 31.92348333911416 21.774902599759624 96-97 17.031369733732348 29.326869560307482 31.643261032644205 21.998499673315962 98-99 16.82045027582641 29.361981529834342 31.46421516315259 22.353353031186654 100-101 16.87405120163649 28.67246472204342 32.471207347429996 21.982276728890092 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3903.0 1 2551.5 2 1466.0 3 1718.5 4 1433.0 5 1489.0 6 2819.0 7 5957.5 8 8381.0 9 8258.5 10 6206.5 11 4156.0 12 3714.5 13 3496.5 14 3108.5 15 2776.5 16 2560.5 17 2428.5 18 2342.5 19 2285.5 20 2295.5 21 2380.5 22 2520.0 23 2716.0 24 3023.0 25 3498.5 26 4250.0 27 5035.5 28 5461.0 29 6451.5 30 8303.5 31 10108.0 32 13078.5 33 16978.5 34 20121.0 35 22362.5 36 26671.5 37 31105.0 38 31202.5 39 29831.5 40 27718.0 41 24986.5 42 22374.5 43 22110.0 44 22332.0 45 20672.5 46 18404.5 47 17374.0 48 22463.0 49 21564.0 50 14682.0 51 10863.5 52 7703.5 53 6515.0 54 5874.5 55 5357.5 56 4883.0 57 4356.5 58 3791.5 59 3429.5 60 3193.5 61 3031.5 62 2755.5 63 1923.5 64 1187.5 65 922.0 66 723.5 67 557.0 68 436.5 69 289.5 70 181.5 71 140.0 72 134.5 73 128.0 74 94.5 75 71.0 76 53.0 77 32.0 78 20.0 79 12.5 80 9.5 81 5.5 82 2.5 83 3.0 84 2.0 85 0.0 86 1.0 87 2.5 88 1.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.29103111161301254 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0015325917740153098 16-17 6.453017995853936E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0017745799488598324 36-37 0.009114887919143685 38-39 0.0034684971727714903 40-41 0.00443644987214958 42-43 4.8397634968904525E-4 44-45 0.0018552426738080066 46-47 0.0 48-49 4.03313624740871E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0011292781492744388 58-59 0.002903858098134271 60-61 0.0011292781492744388 62-63 0.0015325917740153098 64-65 0.0 66-67 6.453017995853936E-4 68-69 0.001935905398756181 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.25683011623498664 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.10445822880788559 88-89 0.367660700313778 90-91 0.2688488622522646 92-93 0.0 94-95 0.0 96-97 0.0 98-99 8.06627249481742E-5 100-101 3.226508997926968E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 619865.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 30.991267968878827 #Duplication Level Percentage of deduplicated Percentage of total 1 73.04800871800857 22.638504127728005 2 12.122342816241567 7.513735492575112 3 4.608992080836473 4.285155259309308 4 2.409533319673794 2.9869797115981083 5 1.4444557032403855 2.2382756884149044 6 0.9749890610668879 1.812968835494969 7 0.70453848800709 1.5284178753351512 8 0.49711765537415586 1.2325045175808975 9 0.4266221406985836 1.1899404975461876 >10 2.998962402206145 19.196521378895778 >50 0.4640855914292687 9.955991884891574 >100 0.2831443552203048 15.526421703618862 >500 0.009907445210286909 1.910300286834432 >1k 0.005214444847519426 2.7461071630299894 >5k 0.0015643334542558276 3.4503698015567172 >10k+ 5.214444847519425E-4 1.7878057755899943 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11063 1.7847434522033023 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8824 1.4235357698853783 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7371 1.189129891185984 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5156 0.8317940196655724 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2721 0.43896654916796396 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2144 0.345881764577771 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2143 0.3457204391278746 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1784 0.28780460261508556 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1743 0.28119025916933527 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1404 0.22650093165447316 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1337 0.2156921265114178 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1306 0.210691037564631 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1276 0.20585127406774054 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1135 0.18310438563235543 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 862 0.13906253781065234 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 765 0.12341396917070653 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 755 0.12180071467174304 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 753 0.12147806377195035 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 713 0.11502504577609642 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 676 0.10905600412993152 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 641 0.10340961338355932 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 639 0.10308696248376661 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.613254498963484E-4 0.0 10-11 0.0 0.0 0.0 1.613254498963484E-4 0.0 12-13 0.0 0.0 0.0 1.613254498963484E-4 0.0 14-15 0.0 0.0 0.0 1.613254498963484E-4 0.0 16-17 0.0 0.0 0.0 1.613254498963484E-4 0.0 18-19 0.0 0.0 0.0 1.613254498963484E-4 0.0 20-21 0.0 0.0 0.0 3.226508997926968E-4 0.0 22-23 0.0 0.0 0.0 0.0012906035991707872 0.0 24-25 0.0 0.0 0.0 0.00403313624740871 0.0 26-27 0.0 0.0 0.0 0.008953562469247337 0.0 28-29 0.0 0.0 0.0 0.017181160413961106 0.0 30-31 0.0 0.0 0.0 0.0428319069474805 0.0 32-33 0.0 0.0 0.0 0.08340525759641212 0.0 34-35 0.0 0.0 0.0 0.14269236043332015 0.0 36-37 0.0 0.0 0.0 0.2229517717567535 0.0 38-39 0.0 0.0 0.0 0.3332983794858558 0.0 40-41 0.0 0.0 0.0 0.48437966331378607 0.0 42-43 0.0 0.0 0.0 0.6178764731030144 0.0 44-45 0.0 0.0 0.0 0.7724262541037161 0.0 46-47 0.0 0.0 0.0 0.9683560130028313 0.0 48-49 0.0 0.0 0.0 1.1838868140643526 0.0 50-51 0.0 0.0 0.0 1.3987723133262888 0.0 52-53 0.0 0.0 0.0 1.6200301678591305 0.0 54-55 0.0 0.0 0.0 1.857823881006348 0.0 56-57 0.0 0.0 0.0 2.1354649802779635 0.0 58-59 0.0 0.0 0.0 2.4451291813540044 0.0 60-61 0.0 0.0 0.0 2.7941567922047543 0.0 62-63 0.0 0.0 0.0 3.129633065264211 0.0 64-65 0.0 0.0 0.0 3.4701104272704537 0.0 66-67 0.0 0.0 0.0 3.869068264864124 0.0 68-69 0.0 0.0 0.0 4.320537536399055 0.0 70-71 0.0 0.0 0.0 4.838230905116436 0.0 72-73 0.0 0.0 0.0 5.353181741185581 0.0 74-75 0.0 0.0 0.0 5.886685003992805 0.0 76-77 0.0 0.0 0.0 6.433094302791737 0.0 78-79 0.0 0.0 0.0 7.062424882837393 0.0 80-81 0.0 0.0 0.0 7.751365216619748 0.0 82-83 0.0 0.0 0.0 8.442402781250756 0.0 84-85 0.0 0.0 0.0 9.174175021980592 0.0 86-87 0.0 0.0 0.0 9.931678671968896 0.0 88-89 0.0 0.0 0.0 10.688053043807926 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTACTC 110 1.6298145E-9 38.83308 8 AGGACCG 50 0.001621495 37.970123 5 GTATGGT 140 4.4201443E-10 34.01439 1 CGCAGAA 75 3.089589E-4 31.644321 9 GTGGTAC 160 1.9172148E-9 29.664158 6 TACCTGG 275 0.0 29.34055 2 CTGACCG 65 0.005864519 29.207787 4 TGACCGT 65 0.005864519 29.207787 5 CATATAG 180 2.1282176E-10 29.004955 3 GACAGAC 115 3.8845083E-6 28.89031 7 ACGCCGA 70 0.008420886 27.121517 6 CTCCGAC 70 5.8467594E-7 27.121517 94-95 ACCGTGC 70 0.008420886 27.121517 7 ACGGAGG 175 4.993126E-9 27.121515 8 TGGTACT 180 6.7429937E-9 26.368141 7 ACACGGA 185 9.025825E-9 25.655487 6 TATAGGT 190 1.198714E-8 24.980343 3 TGGGAGT 190 1.198714E-8 24.980343 6 GTAGATA 195 1.5799742E-8 24.33982 6 CTACACG 215 1.6734703E-9 24.283216 4 >>END_MODULE