FastQCFastQC Report
Fri 27 May 2016
SRR939298_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939298_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences630787
Sequences flagged as poor quality0
Sequence length101
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113361.7971201055189785No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82951.3150239304234235No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69251.0978349268453536No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA53100.841805554014271No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28970.45926754990194785No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA22010.34892919479951234No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20210.32039341330750315No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA17350.2750532271590886No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16540.2622121254876844No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15500.2457247850700791No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT13280.21053065456326778No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT12890.2043479019066658No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12310.19515303898146286No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11500.1823119373100587No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG8540.1353864299676436No Hit
TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC8010.1269842276394409No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT7960.12619156704244064No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT7780.12333798889323973No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG7290.11556991504263721No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC6520.10336294184883328No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT6340.10050936369963236No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGGT801.0747726E-535.6349561
GCATACC550.00260344434.4783678
GGGATAG1255.7516445E-934.1335837
TCGACCG501.3159442E-633.1959618-19
GTTCGAC452.4427709E-531.61018416-17
GGACAAT4200.030.4764166
TAGGACA6000.030.0535164
GACAATG4200.029.347667
TGGGATA3100.029.0563686
GTCGGGA1802.1282176E-1028.9990062
GATAGTC1005.015628E-528.4446537
GATTGGC1852.9649527E-1028.1883969
CATGGGG10150.026.648444
AAGTTCG559.598231E-525.86082614-15
GGTACTC1109.5959425E-525.8587768
GGGATTC1301.0163823E-525.5272527
GGTCGGG2059.513315E-1025.4949261
GTATAGG3800.025.0069871
AGGCTTA3250.024.7979056
GTACATG60250.024.2889441
Produced by FastQC (version 0.11.5)