FastQCFastQC Report
Fri 27 May 2016
SRR939298_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939298_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences630787
Sequences flagged as poor quality0
Sequence length101
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113381.7974371697577787No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88561.403960449406852No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76201.2080147498283889No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA52690.835305737118869No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27800.44071929193214193No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA21330.3381490106803089No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21220.33640515736690835No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18530.2937600172482946No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA17670.2801262549798902No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13660.21655487510046975No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13590.21544515026466937No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT13110.20783560853346694No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT12410.19673836017546337No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11840.18770202936966043No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG8490.13459376937064335No Hit
TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC8040.12745982399764103No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT7370.11683817199783762No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT7360.11667963987843756No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA7240.11477725444563695No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC6880.10907009814723513No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG6830.10827743755023488No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT6540.10368000608763338No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG156.644563E-494.899012
GGATCGA156.644563E-494.899018
TCACGCA200.002082408471.1742554
TAGGACG250.005041406556.9394074
GTGAGTC500.001623092737.9626129
GGGTATG1553.6379788E-1133.6738437
CATTCCG902.4275263E-531.6355119
GGTATGG1201.5180922E-731.6330038
TAGGGTC753.096139E-431.6330034
AGGCTTA4150.027.4406786
CGTCACT908.8539434E-426.4341771
CATATAG2007.021299E-1026.1698341
ATACTGG1109.538702E-525.881556
CATGGGT4950.025.8815464
CTTACTA4350.025.0902339
ATGGGAT15750.025.0051365
GAGTACT62450.024.42950612-13
GTATAGG4100.024.3710231
GTATCAA110150.024.2767261
CCTTTAG1401.754383E-523.7907581
Produced by FastQC (version 0.11.5)