##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939298_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 630787 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.17505750752631 33.0 31.0 34.0 30.0 34.0 2 32.36102361018854 34.0 31.0 34.0 30.0 34.0 3 32.406344772482626 34.0 31.0 34.0 30.0 34.0 4 36.00529021682438 37.0 35.0 37.0 35.0 37.0 5 35.95643537358887 37.0 35.0 37.0 35.0 37.0 6 36.02297130410107 37.0 35.0 37.0 35.0 37.0 7 35.96529414842094 37.0 35.0 37.0 35.0 37.0 8 35.964856599771394 37.0 35.0 37.0 35.0 37.0 9 37.619529254724654 39.0 37.0 39.0 35.0 39.0 10-11 37.54635399905197 39.0 37.0 39.0 35.0 39.0 12-13 37.465163359422434 39.0 37.0 39.0 34.0 39.0 14-15 38.601374156410955 40.0 38.0 41.0 34.0 41.0 16-17 38.57881345049914 40.0 38.0 41.0 34.0 41.0 18-19 38.780846783462565 40.0 38.0 41.0 34.5 41.0 20-21 38.82556710902412 40.0 38.0 41.0 34.5 41.0 22-23 38.740725474684794 40.0 38.0 41.0 34.0 41.0 24-25 38.65762848631947 40.0 38.0 41.0 34.0 41.0 26-27 38.43638026782416 40.0 38.0 41.0 34.0 41.0 28-29 38.24508035200472 40.0 37.5 41.0 34.0 41.0 30-31 37.91520592529649 40.0 37.0 41.0 33.0 41.0 32-33 37.557147658401334 40.0 35.5 41.0 33.0 41.0 34-35 37.39432328186852 40.0 35.0 41.0 33.0 41.0 36-37 37.21912468075594 40.0 35.0 41.0 33.0 41.0 38-39 37.053288193954536 40.0 35.0 41.0 32.5 41.0 40-41 36.821079857384504 39.5 35.0 41.0 31.5 41.0 42-43 36.569966565576024 39.0 35.0 41.0 31.0 41.0 44-45 36.203031292655055 39.0 35.0 41.0 30.0 41.0 46-47 35.692124282840325 39.0 35.0 41.0 28.0 41.0 48-49 35.237888542408136 39.0 35.0 41.0 25.5 41.0 50-51 35.01120187955681 39.0 35.0 41.0 24.5 41.0 52-53 34.798976516637154 38.5 35.0 40.0 24.0 41.0 54-55 34.549941580914 38.0 35.0 40.0 23.5 41.0 56-57 34.340404130078774 38.0 34.0 40.0 23.0 41.0 58-59 34.08967290067804 38.0 34.0 40.0 22.5 41.0 60-61 33.770722129657074 38.0 34.0 40.0 21.5 41.0 62-63 33.393936463497184 37.0 33.0 40.0 19.5 41.0 64-65 32.958222030574504 36.5 33.0 39.5 16.5 41.0 66-67 32.59049647503832 36.0 33.0 39.0 12.5 40.5 68-69 32.20429479364667 36.0 32.0 39.0 8.0 40.0 70-71 31.834402896698887 35.0 32.0 38.5 4.5 40.0 72-73 31.338555645566572 35.0 31.5 37.5 2.0 40.0 74-75 30.831546147907297 35.0 31.0 37.0 2.0 39.0 76-77 28.811295254975136 33.0 28.0 35.0 2.0 37.5 78-79 29.758114862861788 34.0 30.0 36.0 2.0 38.0 80-81 29.763076918199012 34.0 30.0 36.0 2.0 37.5 82-83 29.418038894270175 34.0 30.0 35.0 2.0 37.0 84-85 29.001812022124742 34.0 29.5 35.0 2.0 36.5 86-87 28.45447670925368 34.0 29.0 35.0 2.0 36.0 88-89 27.90917694879571 34.0 28.5 35.0 2.0 36.0 90-91 27.461215909649376 34.0 27.0 35.0 2.0 35.0 92-93 27.118517819802882 33.0 26.0 35.0 2.0 35.0 94-95 26.73456570918551 33.0 25.5 35.0 2.0 35.0 96-97 26.195032554570716 33.0 25.0 35.0 2.0 35.0 98-99 25.513089204438266 33.0 22.0 35.0 2.0 35.0 100-101 22.399486672997384 29.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3078565641808879 1101 2 0.16887031440725764 1101 3 0.3333753844927543 1101 4 0.20778077297639896 1101 5 0.26003015030652676 1101 6 0.06433752339562204 1101 7 0.14786752844958784 1101 8 0.25860952318561203 1101 9 0.16955774086910935 1101 10-11 0.32690844836385224 1101 12-13 0.24583640938899975 1101 14-15 0.33333818490716993 1101 16-17 0.2668050213345694 1101 18-19 0.4550007256966211 1101 20-21 0.4768184548925447 1101 22-23 0.326520426283345 1101 24-25 0.324498722006652 1101 26-27 0.5011143959632349 1101 28-29 0.48854227319014143 1101 30-31 0.4235772821876296 1101 32-33 0.30041851750696935 1101 34-35 0.3818072022928902 1101 36-37 0.24197748954958342 1101 38-39 0.535058731949448 1101 40-41 0.47997289261912357 1101 42-43 0.4319297313254964 1101 44-45 0.47319678854542246 1101 46-47 0.5739335544520756 1101 48-49 0.8510919089582814 1101 50-51 0.8565377767336457 1101 52-53 0.8409710769586951 1101 54-55 0.9188357838594143 1101 56-57 0.884006177893248 1101 58-59 0.857415543327626 1101 60-61 0.8455421963079317 1101 62-63 0.7472821205528817 1101 64-65 0.664437873197592 1101 66-67 0.9740947224519729 1101 68-69 1.2008094240063159 1101 70-71 1.0713293915944675 1101 72-73 0.7007502569847581 1101 74-75 0.5462263705811701 1101 76-77 0.5496398506317668 1101 78-79 1.0215907025573614 1101 80-81 0.829679254792957 1101 82-83 1.1344193296599023 1101 84-85 1.1337182939069095 1101 86-87 0.9516056118772447 1101 88-89 1.0764761462092522 1101 90-91 1.1854724300072377 1101 92-93 1.0417091573047657 1101 94-95 1.0100724266984429 1101 96-97 0.7197767200264202 1101 98-99 0.8430004196479608 1101 100-101 0.34465030658985896 1102 1 0.21326143632652617 1102 2 0.20592440258993605 1102 3 -0.020956800080533355 1102 4 0.113188933557268 1102 5 0.18372201077164618 1102 6 0.16641384140046256 1102 7 0.24502105891365034 1102 8 0.3480878458205723 1102 9 0.386459640269301 1102 10-11 0.25501503312656126 1102 12-13 0.2845166287933978 1102 14-15 0.38111421616046215 1102 16-17 0.41222455990133255 1102 18-19 0.30138147388140624 1102 20-21 0.1741163476632437 1102 22-23 0.23998032052726614 1102 24-25 0.24388766497202852 1102 26-27 0.4461958833882633 1102 28-29 0.47415372483698803 1102 30-31 0.37883812087014945 1102 32-33 0.5155117775301221 1102 34-35 0.40698346242127315 1102 36-37 0.6387337770377428 1102 38-39 0.4476356813338569 1102 40-41 0.4972585524107629 1102 42-43 0.3434527372183709 1102 44-45 0.43418936983555056 1102 46-47 0.18509910743534874 1102 48-49 0.5831452736852114 1102 50-51 0.47941015913919927 1102 52-53 0.5188145869029199 1102 54-55 0.4165651193324251 1102 56-57 0.5834941685277855 1102 58-59 0.5693551939642632 1102 60-61 0.5593365244148387 1102 62-63 0.47453601406019885 1102 64-65 0.5411379942120149 1102 66-67 0.3666796298496351 1102 68-69 0.5079739378440493 1102 70-71 0.41654767792509517 1102 72-73 0.5221438300669377 1102 74-75 0.287418296814991 1102 76-77 0.5372358729405136 1102 78-79 0.6163913576136615 1102 80-81 0.7300857053875056 1102 82-83 0.6145423829062473 1102 84-85 0.7837350649265886 1102 86-87 0.8805556802766965 1102 88-89 1.0986138921842041 1102 90-91 1.201544657198653 1102 92-93 1.0916900843804278 1102 94-95 1.021504671778942 1102 96-97 0.9902373640335789 1102 98-99 0.8638934928879891 1102 100-101 0.6979696531120148 1103 1 0.26807072753418026 1103 2 0.2928865726169576 1103 3 0.15802755868063656 1103 4 0.18012538128123623 1103 5 0.1864826114783611 1103 6 0.0295075329406842 1103 7 0.24528088015663485 1103 8 0.13367036504734386 1103 9 0.3027777134349634 1103 10-11 0.16922530645850742 1103 12-13 0.17666158306485613 1103 14-15 0.2913557200058179 1103 16-17 0.1137711458502082 1103 18-19 0.2839149908217493 1103 20-21 0.4286664671810172 1103 22-23 0.35184960445992175 1103 24-25 0.4568858722054472 1103 26-27 0.3644983412972209 1103 28-29 0.45594999900036015 1103 30-31 0.3703224796313265 1103 32-33 0.3793849327992973 1103 34-35 0.21185770893967515 1103 36-37 0.23551719004959182 1103 38-39 0.21237404134216575 1103 40-41 0.3231620807832485 1103 42-43 0.1330982111323209 1103 44-45 0.21790117611283222 1103 46-47 0.3538724913472109 1103 48-49 0.309965971045429 1103 50-51 -0.016595089249911155 1103 52-53 0.11037884069631332 1103 54-55 0.2132582444623381 1103 56-57 0.3873453689019257 1103 58-59 -0.10660874284720734 1103 60-61 -0.07284753295368773 1103 62-63 -0.0680162100096382 1103 64-65 0.26538321350113847 1103 66-67 0.19235581463450302 1103 68-69 0.15167132404234707 1103 70-71 0.34269673737220074 1103 72-73 0.4739697238520151 1103 74-75 0.5186104865884289 1103 76-77 0.6716933661851598 1103 78-79 0.8336636242941857 1103 80-81 0.6086842296028472 1103 82-83 0.5425101910542445 1103 84-85 0.32420547128701216 1103 86-87 0.42481622901916083 1103 88-89 0.6237905706294313 1103 90-91 0.8007606465979471 1103 92-93 0.5289497497306677 1103 94-95 0.5455218979273511 1103 96-97 0.5796808269311065 1103 98-99 0.6567152814974264 1103 100-101 0.23611790711326108 1104 1 0.05693376441269038 1104 2 0.20602796514172184 1104 3 0.09790711872642532 1104 4 0.12481382999471435 1104 5 0.04683820795474247 1104 6 0.008144371640725012 1104 7 0.005066626436438071 1104 8 0.08747268426299115 1104 9 0.09702819867857926 1104 10-11 0.10237677711993598 1104 12-13 0.16110890302702785 1104 14-15 0.07683449370809825 1104 16-17 0.021444216073660982 1104 18-19 0.0186298168805763 1104 20-21 0.11960360946937953 1104 22-23 -0.03325652702866222 1104 24-25 0.01448366745752594 1104 26-27 0.1256309496682988 1104 28-29 0.2121275235113842 1104 30-31 0.3991104903813394 1104 32-33 0.3609705596345165 1104 34-35 0.5326566190078523 1104 36-37 0.34989782011976445 1104 38-39 0.7675145131482708 1104 40-41 0.8003214148796971 1104 42-43 0.9797798913194526 1104 44-45 1.0690278122547738 1104 46-47 1.199313067667326 1104 48-49 1.218799271199714 1104 50-51 0.9831125203653812 1104 52-53 1.144759595187928 1104 54-55 1.011337799531269 1104 56-57 0.8897933607398798 1104 58-59 0.8438995187364213 1104 60-61 0.9006786950859293 1104 62-63 0.8945080521712185 1104 64-65 0.7819468177414208 1104 66-67 0.785292409468525 1104 68-69 0.8015867174629321 1104 70-71 0.8206617002946075 1104 72-73 0.6697593023121762 1104 74-75 0.723999179167933 1104 76-77 0.9726128406289973 1104 78-79 1.070810889510927 1104 80-81 1.1458893507893286 1104 82-83 1.123306363850741 1104 84-85 1.2443424592927883 1104 86-87 1.1598638824307983 1104 88-89 1.2869941738743478 1104 90-91 1.4306379670578089 1104 92-93 1.1707342020274858 1104 94-95 1.117235305581758 1104 96-97 1.0712232795265386 1104 98-99 1.3255365127720005 1104 100-101 0.9148555271799488 1105 1 -0.050478972880632966 1105 2 0.04069422356180752 1105 3 0.23077913342329737 1105 4 0.1246882941710794 1105 5 0.001385054249908535 1105 6 0.13106537592732082 1105 7 0.17747630188040375 1105 8 0.06570293533463456 1105 9 0.0933937834918055 1105 10-11 0.10206906125955584 1105 12-13 0.03230914797497775 1105 14-15 0.2395013644980608 1105 16-17 0.31901004583484394 1105 18-19 0.1659690697893339 1105 20-21 0.21046705098805774 1105 22-23 0.10909956605156168 1105 24-25 0.12335537596946722 1105 26-27 0.23746346650846561 1105 28-29 0.2668886992615356 1105 30-31 0.1748131848087766 1105 32-33 0.24492034530505435 1105 34-35 0.137443820477543 1105 36-37 0.05352917345717856 1105 38-39 -0.1816450092032298 1105 40-41 -0.09445660104191944 1105 42-43 -0.02973939833150041 1105 44-45 0.07917018824986854 1105 46-47 -0.09013426852373385 1105 48-49 0.007753649644293148 1105 50-51 0.13514865018779432 1105 52-53 0.027121811966864584 1105 54-55 -0.07736550726603753 1105 56-57 -0.10872929694536282 1105 58-59 0.10643319907935478 1105 60-61 -0.14922485665933038 1105 62-63 0.029309031963009602 1105 64-65 -0.1366401387803151 1105 66-67 -0.22452081772069477 1105 68-69 -0.06192216190019195 1105 70-71 0.09083316385860485 1105 72-73 -0.014833471723342484 1105 74-75 0.11726004873315077 1105 76-77 0.031084977799849867 1105 78-79 -0.03477392371626564 1105 80-81 0.22363429873788832 1105 82-83 0.24079717830266745 1105 84-85 0.1696318040570226 1105 86-87 0.16052371096723306 1105 88-89 -0.02029915129405424 1105 90-91 -0.04408034280134743 1105 92-93 -0.39296145014642647 1105 94-95 -0.3422181543080143 1105 96-97 -0.13356698379250886 1105 98-99 0.19629278956318075 1105 100-101 0.1854280930097083 1106 1 0.062221293901401964 1106 2 0.033002248653737354 1106 3 0.0863101738732226 1106 4 -0.033330308467895975 1106 5 -0.004037110289715429 1106 6 0.0071458431563868885 1106 7 -0.03029354453966704 1106 8 -0.059439468587775934 1106 9 0.15101004998543033 1106 10-11 0.10671395274415829 1106 12-13 0.06857275746376956 1106 14-15 0.0804286123169291 1106 16-17 0.04466438284543983 1106 18-19 -0.03758850465727903 1106 20-21 0.08184638619607654 1106 22-23 0.06172484545166412 1106 24-25 0.06922025447548918 1106 26-27 0.1928257853490436 1106 28-29 0.17554108948433367 1106 30-31 0.15557026895326231 1106 32-33 0.25542434703553596 1106 34-35 0.19561383994542325 1106 36-37 0.3497985512061135 1106 38-39 0.22135592813904026 1106 40-41 0.5515870935287026 1106 42-43 0.5363836098161698 1106 44-45 0.4706187425796742 1106 46-47 0.7164588528260438 1106 48-49 0.535124044049077 1106 50-51 0.37051529522348403 1106 52-53 0.2845462838105419 1106 54-55 0.4209198774048133 1106 56-57 0.47560987675137056 1106 58-59 0.5607889565240143 1106 60-61 0.41316176097713253 1106 62-63 0.6624231714107012 1106 64-65 0.6128962373102453 1106 66-67 0.7177749498184127 1106 68-69 0.7657856757232793 1106 70-71 0.4412967877680387 1106 72-73 0.5292879501527814 1106 74-75 0.3809228939274689 1106 76-77 0.15604135533247288 1106 78-79 0.20670419294679832 1106 80-81 0.4634958604943229 1106 82-83 0.2556216068843966 1106 84-85 0.23556445961978767 1106 86-87 0.2227113236285625 1106 88-89 0.5125856998127283 1106 90-91 0.5072297732848909 1106 92-93 0.5602449814636365 1106 94-95 0.47722088239254035 1106 96-97 0.41986008376823847 1106 98-99 0.939838827489492 1106 100-101 0.834252088971926 1107 1 0.08097965264531126 1107 2 0.04278398513262971 1107 3 0.0499516439422365 1107 4 -0.05093582925108109 1107 5 0.050052655932937284 1107 6 -0.0182754375369214 1107 7 0.04056185587759842 1107 8 0.11030321583954361 1107 9 0.002173587138372568 1107 10-11 0.01569458720625505 1107 12-13 0.050829484580866335 1107 14-15 0.022972612744901255 1107 16-17 0.05018115382922872 1107 18-19 0.28324817217362863 1107 20-21 0.14433100610726513 1107 22-23 0.09622439073599054 1107 24-25 0.01707907863426783 1107 26-27 0.049546215456572895 1107 28-29 0.09181857303432395 1107 30-31 0.17918204876389154 1107 32-33 0.11421908303297101 1107 34-35 0.1001997812068538 1107 36-37 0.30348709772085414 1107 38-39 0.14565513104922445 1107 40-41 -0.24584510034065232 1107 42-43 -0.2915626802389326 1107 44-45 -0.2564889982858958 1107 46-47 -0.45310060793466533 1107 48-49 -0.20073162384800014 1107 50-51 -0.2947952488304395 1107 52-53 -0.2883901094917647 1107 54-55 -0.40311585789716986 1107 56-57 -0.15371527169991595 1107 58-59 0.04015690174414743 1107 60-61 -0.1784955438963607 1107 62-63 -0.12644833129084532 1107 64-65 -0.08861769838761546 1107 66-67 -0.026554478309755325 1107 68-69 -0.07638920257340232 1107 70-71 -0.2902537926631368 1107 72-73 -0.28308031604311523 1107 74-75 -0.29454920792883854 1107 76-77 -0.061530730475048756 1107 78-79 0.1649385587521799 1107 80-81 -0.14119038569408815 1107 82-83 0.07491358783983415 1107 84-85 -0.0931802576540548 1107 86-87 -0.06035987949560351 1107 88-89 0.42442034379710947 1107 90-91 0.4681777217147811 1107 92-93 0.4884761375113591 1107 94-95 0.5032587040370053 1107 96-97 0.47047929951745004 1107 98-99 0.6704933505730004 1107 100-101 0.4701826512144791 1108 1 -0.01863632277711247 1108 2 0.1504388750455874 1108 3 0.1314416593032206 1108 4 0.04783529210068593 1108 5 0.14651962981926658 1108 6 0.09094115232483091 1108 7 0.04030583949255373 1108 8 0.021465530227665397 1108 9 0.13340758611430914 1108 10-11 0.19535408541414512 1108 12-13 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-1.0791209688983443 2316 54-55 -1.170368667340135 2316 56-57 -1.132337229821026 2316 58-59 -1.014392778909098 2316 60-61 -1.2116995215825028 2316 62-63 -1.3621282569113475 2316 64-65 -1.1840619541884045 2316 66-67 -1.041470801375489 2316 68-69 -1.050552433230699 2316 70-71 -1.0155044192295506 2316 72-73 -1.3597599182745022 2316 74-75 -1.2713015727117636 2316 76-77 -1.1365994506626151 2316 78-79 -1.1135055353540508 2316 80-81 -1.1063896742380273 2316 82-83 -1.169898037998955 2316 84-85 -1.1639074789330621 2316 86-87 -1.3229033349083288 2316 88-89 -1.6115991403972494 2316 90-91 -2.208817184906607 2316 92-93 -2.012536474664241 2316 94-95 -1.5026572956396045 2316 96-97 -1.688803989349882 2316 98-99 -1.8711126806340523 2316 100-101 -1.2511448611147316 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 3.0 4 9.0 5 37.0 6 98.0 7 212.0 8 354.0 9 613.0 10 1045.0 11 1565.0 12 2110.0 13 2969.0 14 3427.0 15 4985.0 16 6851.0 17 9900.0 18 8898.0 19 5138.0 20 4377.0 21 4500.0 22 4963.0 23 5195.0 24 5962.0 25 6579.0 26 7606.0 27 8889.0 28 10649.0 29 12471.0 30 14813.0 31 18270.0 32 21599.0 33 27252.0 34 36955.0 35 59526.0 36 74534.0 37 123266.0 38 123407.0 39 11759.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.34898479578275 22.99725293941841 17.22079499424516 18.43296727055367 2 14.418654791554042 24.580722177216714 40.659049726769894 20.341573304459352 3 16.904438423746843 31.431212120731722 32.40776997623603 19.256579479285403 4 10.782720633113872 20.56018909711202 40.3882768668346 28.268813402939504 5 10.088667014380448 41.67476501576602 34.72725341517818 13.50931455467535 6 24.322473354714035 41.975817510506715 18.54445319894037 15.157255935838881 7 23.562153310071388 37.66120734891493 22.950853457664792 15.825785883348894 8 24.736876314825764 35.098377106693704 21.173549867070818 18.991196711409717 9 26.83901221807044 17.719452049582507 22.64013050364069 32.80140522870636 10-11 22.48445196238984 30.03834891968287 27.669324193428206 19.807874924499078 12-13 25.228960013443526 28.748769394423157 28.33230551675922 17.689965075374094 14-15 22.36698276005571 29.12860162958653 29.153967311562706 19.35044829879505 16-17 17.41065235846701 32.725488501943225 30.171985341969194 19.69187379762057 18-19 17.23022192911395 29.888060470491624 35.48725639558203 17.3944612048124 20-21 18.955447718485 28.803383709556474 35.23415986695985 17.00700870499868 22-23 18.609689166073494 28.716349576005847 35.06334150830629 17.61061974961437 24-25 18.018947788949024 29.697155612204458 35.46440472680116 16.819491872045354 26-27 17.820714036589617 29.411918576999906 35.70721963283553 17.060147753574938 28-29 17.141681740429018 29.096668130446567 36.51700177714506 17.244648351979354 30-31 17.528896442063644 29.75251550840458 35.730761730980504 16.98782631855127 32-33 17.05544026747539 30.425801419496594 35.162186284752224 17.35657202827579 34-35 17.469584407431014 29.715407713210375 35.50855948836487 17.30644839099375 36-37 17.945983659521836 29.407469366558708 34.89687104817706 17.749675925742398 38-39 17.55006785637279 30.062672019075887 35.1775839326256 17.209676191925727 40-41 17.040503085617157 30.045126754488894 35.74202274413796 17.172347415755993 42-43 17.074485457087864 30.192911975191596 34.75736975112326 17.975232816597277 44-45 17.17208387994277 30.30521614027022 34.304058847542535 18.21864113224447 46-47 17.77256030958152 30.425008758899597 33.32567094756233 18.47675998395655 48-49 17.640582320180982 30.646953726059667 33.04998359192564 18.66248036183371 50-51 17.751871867999817 30.732878134774495 32.83200192775057 18.683248069475116 52-53 17.54427405764545 31.38856698061311 32.68726210273832 18.379896859003118 54-55 17.627107090031977 31.08228292593221 32.453744290862055 18.836865693173767 56-57 17.930933896862175 31.563427908311365 31.53108735595375 18.97455083887271 58-59 17.630655281856487 30.74485321394713 32.589071977323385 19.035419526872992 60-61 17.7790495909368 31.645820468775888 32.04081357641171 18.534316363875607 62-63 18.14327997602964 31.216083604558808 32.42704655671796 18.213589862693592 64-65 18.053003628800212 30.77710859608711 32.6102154927099 18.559672282402776 66-67 17.79451700811843 31.230668989690656 31.79512260081454 19.179691401376374 68-69 17.68589014438416 31.134503572943128 32.23551699674611 18.94408928592661 70-71 18.015590048621803 31.02140659208259 32.04520701916812 18.91779634012749 72-73 17.806644715252535 30.637441798895665 31.824768107142347 19.731145378709453 74-75 17.35466964284299 31.535367723177554 31.48757028917844 19.622392344801018 76-77 17.407132562437333 30.953693801633854 32.35006221089414 19.28911142503468 78-79 17.000984484461473 30.546285830240638 32.44320190492195 20.009527780375944 80-81 17.15413346692497 31.605810602145283 31.468361293513723 19.771694637416015 82-83 17.36109019367869 30.861289151488535 31.495100564849942 20.28252008998283 84-85 16.844830346852426 30.237385995589637 31.6852598420703 21.232523815487635 86-87 16.99195487940254 30.674134925376922 31.753906116719712 20.580004078500824 88-89 17.38029493399903 29.85966093011526 31.978469833981293 20.781574301904413 90-91 16.976426535481114 29.918483686793433 32.15475291734849 20.95033686037696 92-93 17.0793786175048 30.287006548961852 31.71728333018911 20.916331503344235 94-95 16.70278556786998 29.673883577182153 31.970617656990395 21.65271319795747 96-97 16.999399163267473 29.427445397574775 31.6725772725183 21.900578166639452 98-99 16.784825939659502 29.443377875574484 31.535446989237254 22.23634919552876 100-101 16.809187267948243 28.873738478659956 32.452448215401105 21.864626037990696 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4082.0 1 2662.0 2 1477.0 3 1718.0 4 1431.0 5 1484.0 6 2858.0 7 6092.5 8 8669.0 9 8493.0 10 6349.5 11 4260.0 12 3738.0 13 3464.5 14 3079.5 15 2796.5 16 2600.5 17 2491.5 18 2364.0 19 2313.5 20 2333.5 21 2471.0 22 2644.5 23 2842.5 24 3082.5 25 3462.5 26 4289.5 27 5071.5 28 5410.5 29 6563.5 30 8470.5 31 10176.5 32 13171.5 33 17226.0 34 20670.0 35 22991.0 36 27201.0 37 31841.0 38 31790.0 39 30056.0 40 28284.5 41 25663.5 42 22834.0 43 22414.0 44 22551.5 45 21182.5 46 19157.5 47 17806.5 48 22745.0 49 21785.5 50 14736.5 51 10997.0 52 7826.5 53 6571.0 54 5952.5 55 5417.0 56 4868.5 57 4356.5 58 3856.0 59 3493.5 60 3273.5 61 3163.5 62 2781.0 63 1898.5 64 1196.0 65 936.0 66 757.5 67 574.0 68 410.5 69 255.5 70 162.0 71 142.5 72 138.5 73 125.5 74 101.0 75 80.5 76 55.5 77 30.5 78 20.0 79 14.0 80 7.5 81 5.5 82 4.5 83 1.5 84 1.5 85 1.0 86 0.0 87 0.0 88 0.0 89 0.5 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.27758974106948936 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.002140183611900689 16-17 0.0010304587761003317 18-19 0.0 20-21 0.0 22-23 0.0 24-25 1.5853211940005104E-4 26-27 0.0011097248358003574 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.003963302985001276 36-37 0.022907891253307375 38-39 0.006816881134202196 40-41 0.018865322208606074 42-43 0.005231559940201685 44-45 7.133945373002297E-4 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 7.926605970002553E-4 60-61 0.0026157799701008425 62-63 0.0012682569552004083 64-65 0.0 66-67 0.0 68-69 7.133945373002297E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.23399340823447534 78-79 0.0 80-81 0.0014267890746004594 82-83 0.0 84-85 0.0 86-87 0.10360074002793336 88-89 0.3604227734560161 90-91 0.3586789201426155 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 1.5853211940005104E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 630787.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 31.04242814783445 #Duplication Level Percentage of deduplicated Percentage of total 1 73.09360672126039 22.69003034710795 2 12.119292467572794 7.524245312544394 3 4.699800810017503 4.3767968686230745 4 2.350364321425278 2.9184406227631143 5 1.4243716853994897 2.2107977849911755 6 0.9430929970579134 1.7565537958737676 7 0.6996433454477702 1.5203039796121045 8 0.5184607998138221 1.2875425700551482 9 0.38684094361565985 1.0807633977146638 >10 2.988971604056922 18.951339699600176 >50 0.47341020818185914 10.108680646682872 >100 0.2852731060101752 15.73593507977007 >500 0.009713600383859012 1.871889729772757 >1k 0.005112421254662638 2.7163429092658338 >5k 0.0015337263763987915 3.4509743258930565 >10k+ 5.112421254662638E-4 1.799362929729845 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11338 1.7974371697577787 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8856 1.403960449406852 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7620 1.2080147498283889 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5269 0.835305737118869 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2780 0.44071929193214193 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2133 0.3381490106803089 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2122 0.33640515736690835 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1853 0.2937600172482946 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1767 0.2801262549798902 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1366 0.21655487510046975 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1359 0.21544515026466937 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1311 0.20783560853346694 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1241 0.19673836017546337 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1184 0.18770202936966043 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 849 0.13459376937064335 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 804 0.12745982399764103 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 737 0.11683817199783762 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 736 0.11667963987843756 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 724 0.11477725444563695 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 688 0.10907009814723513 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 683 0.10827743755023488 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 654 0.10368000608763338 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 7.926605970002552E-5 0.0 12-13 0.0 0.0 0.0 1.5853211940005104E-4 0.0 14-15 0.0 0.0 0.0 1.5853211940005104E-4 0.0 16-17 0.0 0.0 0.0 1.5853211940005104E-4 0.0 18-19 0.0 0.0 0.0 1.5853211940005104E-4 0.0 20-21 0.0 0.0 0.0 1.5853211940005104E-4 0.0 22-23 0.0 0.0 0.0 3.170642388001021E-4 0.0 24-25 0.0 0.0 0.0 0.0031706423880010206 0.0 26-27 0.0 0.0 0.0 0.006737615074502169 0.0 28-29 0.0 0.0 0.0 0.016011744059405156 0.0 30-31 0.0 0.0 0.0 0.043754864954414094 0.0 32-33 0.0 0.0 0.0 0.08100991301342608 0.0 34-35 0.0 0.0 0.0 0.13713028328104415 0.0 36-37 0.0 0.0 0.0 0.22028037990637095 0.0 38-39 0.0 0.0 0.0 0.3372770840236086 0.0 40-41 0.0 0.0 0.0 0.4725842479315522 0.0 42-43 0.0 0.0 0.0 0.6091596687946961 0.0 44-45 0.0 0.0 0.0 0.7627772924933456 0.0 46-47 0.0 0.0 0.0 0.9492110649078056 0.0 48-49 0.0 0.0 0.0 1.1530833704562713 0.0 50-51 0.0 0.0 0.0 1.3715406309895417 0.0 52-53 0.0 0.0 0.0 1.589997891522812 0.0 54-55 0.0 0.0 0.0 1.8176500149812853 0.0 56-57 0.0 0.0 0.0 2.097142141483575 0.0 58-59 0.0 0.0 0.0 2.400493351955573 0.0 60-61 0.0 0.0 0.0 2.748946950396885 0.0 62-63 0.0 0.0 0.0 3.088681282271194 0.0 64-65 0.0 0.0 0.0 3.4327752474290056 0.0 66-67 0.0 0.0 0.0 3.8178497654517294 0.0 68-69 0.0 0.0 0.0 4.260154378577871 0.0 70-71 0.0 0.0 0.0 4.766743766120735 0.0 72-73 0.0 0.0 0.0 5.268022327663696 0.0 74-75 0.0 0.0 0.0 5.788562541713764 0.0 76-77 0.0 0.0 0.0 6.33430936274844 0.0 78-79 0.0 0.0 0.0 6.977949767512647 0.0 80-81 0.0 0.0 0.0 7.678423937081773 0.0 82-83 0.0 0.0 0.0 8.361935169875093 0.0 84-85 0.0 0.0 0.0 9.097365671771929 0.0 86-87 0.0 0.0 0.0 9.859746633966775 0.0 88-89 0.0 0.0 0.0 10.610158421146917 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATCACG 15 6.644563E-4 94.89901 2 GGATCGA 15 6.644563E-4 94.89901 8 TCACGCA 20 0.0020824084 71.174255 4 TAGGACG 25 0.0050414065 56.939407 4 GTGAGTC 50 0.0016230927 37.962612 9 GGGTATG 155 3.6379788E-11 33.673843 7 CATTCCG 90 2.4275263E-5 31.635511 9 GGTATGG 120 1.5180922E-7 31.633003 8 TAGGGTC 75 3.096139E-4 31.633003 4 AGGCTTA 415 0.0 27.440678 6 CGTCACT 90 8.8539434E-4 26.434177 1 CATATAG 200 7.021299E-10 26.169834 1 ATACTGG 110 9.538702E-5 25.88155 6 CATGGGT 495 0.0 25.881546 4 CTTACTA 435 0.0 25.090233 9 ATGGGAT 1575 0.0 25.005136 5 GAGTACT 6245 0.0 24.429506 12-13 GTATAGG 410 0.0 24.371023 1 GTATCAA 11015 0.0 24.276726 1 CCTTTAG 140 1.754383E-5 23.790758 1 >>END_MODULE