##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939297_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1483037 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.963354926411142 33.0 31.0 34.0 30.0 34.0 2 31.98651820554713 33.0 31.0 34.0 30.0 34.0 3 32.03836316963097 34.0 31.0 34.0 30.0 34.0 4 35.52020954298511 37.0 35.0 37.0 33.0 37.0 5 35.580844577714515 37.0 35.0 37.0 33.0 37.0 6 35.70190629094217 37.0 35.0 37.0 33.0 37.0 7 35.65707126659685 37.0 35.0 37.0 33.0 37.0 8 35.65930317315077 37.0 35.0 37.0 33.0 37.0 9 37.25847028765971 39.0 37.0 39.0 34.0 39.0 10-11 37.16629220983698 39.0 37.0 39.0 34.0 39.0 12-13 37.08596919699239 39.0 37.0 39.0 33.0 39.0 14-15 38.22052180761505 40.0 38.0 41.0 33.0 41.0 16-17 38.18709108403904 40.0 38.0 41.0 33.0 41.0 18-19 38.21494035550023 40.0 38.0 41.0 33.5 41.0 20-21 38.19924250035569 40.0 38.0 41.0 33.5 41.0 22-23 38.208259807408716 40.0 38.0 41.0 33.5 41.0 24-25 38.10213366220802 40.0 38.0 41.0 33.0 41.0 26-27 37.98359548682872 40.0 38.0 41.0 33.0 41.0 28-29 37.85748602361235 40.0 38.0 41.0 33.0 41.0 30-31 37.68902866213048 40.0 37.0 41.0 33.0 41.0 32-33 37.422447653025515 40.0 36.5 41.0 32.5 41.0 34-35 37.10036937716321 40.0 36.0 41.0 31.0 41.0 36-37 36.82512068141253 39.5 35.5 41.0 30.5 41.0 38-39 36.222573341056226 38.5 35.0 40.5 30.0 41.0 40-41 36.23364791303251 39.0 35.0 40.0 30.0 41.0 42-43 36.33671108677666 39.0 35.0 41.0 30.0 41.0 44-45 36.148275464469194 39.0 35.0 41.0 29.5 41.0 46-47 35.75062119151444 39.0 35.0 41.0 27.5 41.0 48-49 35.39797456165962 39.0 35.0 41.0 26.0 41.0 50-51 34.20630267484897 38.0 34.0 40.0 23.5 40.5 52-53 34.33547140091582 38.0 34.5 39.5 23.5 40.5 54-55 34.86231833730379 39.0 35.0 40.5 24.0 41.0 56-57 34.76748017750063 39.0 35.0 41.0 23.5 41.0 58-59 34.57218700544895 38.5 34.5 40.0 23.0 41.0 60-61 34.20721937483691 38.0 34.0 40.0 22.0 41.0 62-63 33.860915809922474 38.0 34.0 40.0 21.5 41.0 64-65 33.46821050317693 37.0 33.5 40.0 20.0 41.0 66-67 33.03893935215372 36.5 33.0 39.0 18.0 41.0 68-69 32.60655499491921 36.0 32.5 39.0 15.0 40.5 70-71 32.09911418258614 35.5 32.0 38.5 10.5 40.0 72-73 31.63308501406236 35.0 31.5 37.5 7.5 40.0 74-75 31.18233833680481 35.0 31.0 37.0 6.0 39.0 76-77 30.715910661702978 35.0 31.0 36.5 2.0 39.0 78-79 30.303492765183876 35.0 31.0 36.0 2.0 38.5 80-81 29.8572453013647 34.0 30.0 36.0 2.0 37.0 82-83 29.40712099563261 34.0 29.5 35.0 2.0 37.0 84-85 28.950994479571314 34.0 29.0 35.0 2.0 36.5 86-87 28.574134023628538 34.0 29.0 35.0 2.0 36.0 88-89 28.104203401533475 34.0 28.5 35.0 2.0 36.0 90-91 27.63967756704654 34.0 27.0 35.0 2.0 35.0 92-93 27.177459834110678 34.0 26.0 35.0 2.0 35.0 94-95 26.65714779874002 33.0 25.0 35.0 2.0 35.0 96-97 26.021252672724955 33.0 24.5 35.0 2.0 35.0 98-99 25.276228442041567 33.0 19.0 35.0 2.0 35.0 100-101 22.648118691576812 29.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.31666793202425225 1101 2 0.49208773839234254 1101 3 0.4977119965878032 1101 4 0.34153131435314776 1101 5 0.35425204213908756 1101 6 0.3767736728457791 1101 7 0.3434679881735647 1101 8 0.2370847922424204 1101 9 0.33440748887022664 1101 10-11 0.5147842052778913 1101 12-13 0.4736348451975161 1101 14-15 0.6920620278985368 1101 16-17 0.7179154280449467 1101 18-19 0.6699593789135534 1101 20-21 0.744538915558671 1101 22-23 0.7521999341650059 1101 24-25 0.7809680313852425 1101 26-27 0.7203595743532034 1101 28-29 0.9176813119636975 1101 30-31 0.8117120205712709 1101 32-33 0.9185017606662456 1101 34-35 0.8004701737703215 1101 36-37 0.9699884102376828 1101 38-39 1.3932417948403995 1101 40-41 1.1064172754431638 1101 42-43 1.0324368042255045 1101 44-45 0.9374171529892763 1101 46-47 1.0602458948489897 1101 48-49 1.182691663013486 1101 50-51 1.2170484561598442 1101 52-53 1.362786942869981 1101 54-55 1.3614133286794114 1101 56-57 1.2973669627912585 1101 58-59 1.3671258981541357 1101 60-61 1.329607767437892 1101 62-63 1.3596667753903944 1101 64-65 1.172429566122382 1101 66-67 1.1841404679159382 1101 68-69 1.2774823075080946 1101 70-71 1.2386370774082351 1101 72-73 1.2151714180084419 1101 74-75 1.1574466158248917 1101 76-77 1.2220868267323333 1101 78-79 1.0830234995300572 1101 80-81 1.1765386986369748 1101 82-83 1.0385345352565416 1101 84-85 0.8742139570072212 1101 86-87 0.942200586659343 1101 88-89 1.006846129321655 1101 90-91 1.2812373928792624 1101 92-93 1.3228514842184005 1101 94-95 1.3909777528776601 1101 96-97 1.495711441439461 1101 98-99 1.5010738801787582 1101 100-101 1.2427875343418542 1102 1 0.29893392589117695 1102 2 0.3984185820781718 1102 3 0.36589769075957435 1102 4 0.27549165666616204 1102 5 0.17803691928823895 1102 6 0.24550741396930675 1102 7 0.21186100273827435 1102 8 0.3240692179739426 1102 9 0.3252204912841634 1102 10-11 0.3354583751399929 1102 12-13 0.3146417372484933 1102 14-15 0.5321098377839348 1102 16-17 0.550668264278066 1102 18-19 0.4641596359908462 1102 20-21 0.5106157106377864 1102 22-23 0.5502933076644112 1102 24-25 0.4835443928943164 1102 26-27 0.37350714861446477 1102 28-29 0.4153827597811457 1102 30-31 0.4940385880486531 1102 32-33 0.39389823838290994 1102 34-35 0.24575201232362076 1102 36-37 0.3291998355743999 1102 38-39 0.6879702682231681 1102 40-41 0.5068160517120077 1102 42-43 0.33943413790497345 1102 44-45 0.2116794980172827 1102 46-47 0.24878666575634867 1102 48-49 0.16116279229667896 1102 50-51 0.05820768362997342 1102 52-53 0.12266305541430711 1102 54-55 0.1853514426407301 1102 56-57 0.11935175809392007 1102 58-59 0.1974810289733071 1102 60-61 0.24685718468730755 1102 62-63 0.24784716937124784 1102 64-65 0.23546307811181322 1102 66-67 0.24831694624555212 1102 68-69 0.11313853063531099 1102 70-71 0.32077651569616705 1102 72-73 0.302672341936276 1102 74-75 0.038472653463461626 1102 76-77 0.01879746335016108 1102 78-79 -0.046913204652586415 1102 80-81 -0.04613692427066596 1102 82-83 -0.3124715094850039 1102 84-85 -0.3370184816034083 1102 86-87 -0.338006445287828 1102 88-89 -0.27192858912824036 1102 90-91 -0.4265931885012044 1102 92-93 -0.24230661110211216 1102 94-95 -0.06956376798868646 1102 96-97 -0.2555509594749168 1102 98-99 0.0015939839471244 1102 100-101 -0.16283211025690747 1103 1 0.3820125111092878 1103 2 0.3722878605771953 1103 3 0.45712349264051966 1103 4 0.2860665077433069 1103 5 0.22187780060454543 1103 6 0.3067065898015926 1103 7 0.3139692426442835 1103 8 0.29037103102483997 1103 9 0.5146603996574939 1103 10-11 0.4050550879151871 1103 12-13 0.556676061826515 1103 14-15 0.652517729957296 1103 16-17 0.6820040535839524 1103 18-19 0.7196058732165369 1103 20-21 0.6633503416220066 1103 22-23 0.5413219232869935 1103 24-25 0.591390221578429 1103 26-27 0.5435091558997343 1103 28-29 0.5313763955061077 1103 30-31 0.6708990167811706 1103 32-33 0.697613192067756 1103 34-35 0.5332610893856611 1103 36-37 0.23424904358228105 1103 38-39 0.8226222108620362 1103 40-41 0.7417174290639252 1103 42-43 0.4749678101152952 1103 44-45 0.46767006377653075 1103 46-47 0.544980498667563 1103 48-49 0.4687925426258204 1103 50-51 0.23838869353061654 1103 52-53 0.3565472114394126 1103 54-55 0.5283466074447531 1103 56-57 0.630510197842888 1103 58-59 0.5908448848502275 1103 60-61 0.73655184117586 1103 62-63 0.7168673780108392 1103 64-65 0.6576781174014741 1103 66-67 0.4873267642320087 1103 68-69 0.500329689134098 1103 70-71 0.21780307680324285 1103 72-73 0.2552083107170908 1103 74-75 0.22580963533295417 1103 76-77 0.31679135884144216 1103 78-79 0.25927189066394263 1103 80-81 0.25546359053896595 1103 82-83 0.1443145906549148 1103 84-85 0.3477710191139032 1103 86-87 0.2756513598047725 1103 88-89 0.20824799293725604 1103 90-91 0.2633399807070411 1103 92-93 0.4870071334885502 1103 94-95 0.6101833140679531 1103 96-97 0.5724553929925698 1103 98-99 0.9308407206326237 1103 100-101 0.8287006881939405 1104 1 0.21276738812370866 1104 2 0.31527344157804293 1104 3 0.253210572086374 1104 4 0.23072582354765814 1104 5 0.3932264011134521 1104 6 0.2829689590410638 1104 7 0.19179225649783405 1104 8 0.31852483368246 1104 9 0.38127617573891825 1104 10-11 0.3511789216726129 1104 12-13 0.30024971181238413 1104 14-15 0.3838465396830557 1104 16-17 0.386522006651532 1104 18-19 0.4464110753652548 1104 20-21 0.5048289958487473 1104 22-23 0.38687747884255685 1104 24-25 0.46839615881337693 1104 26-27 0.41180997580359957 1104 28-29 0.3541951684775526 1104 30-31 0.5297539786132361 1104 32-33 0.43459085675534226 1104 34-35 0.4672249005250535 1104 36-37 0.4719024221517003 1104 38-39 0.8783389199375264 1104 40-41 0.731612820638702 1104 42-43 0.7177696195583181 1104 44-45 0.8409525065246228 1104 46-47 1.023744988348085 1104 48-49 0.9532200235418458 1104 50-51 0.8990841981955882 1104 52-53 0.7431262332092672 1104 54-55 0.8974842947503774 1104 56-57 0.9162014616257608 1104 58-59 0.716434367462611 1104 60-61 0.729079406909527 1104 62-63 0.6506898264134477 1104 64-65 0.6494847331775517 1104 66-67 0.7671345109099832 1104 68-69 0.7821391321649145 1104 70-71 0.7963526951238507 1104 72-73 0.648287191124215 1104 74-75 0.6537429121211922 1104 76-77 0.6324882771337812 1104 78-79 0.338330414836971 1104 80-81 0.5366086287069045 1104 82-83 0.5100652267872299 1104 84-85 0.2558456586389255 1104 86-87 0.21314075759951479 1104 88-89 0.2423757848513084 1104 90-91 0.35115451279638066 1104 92-93 0.3649856463525616 1104 94-95 0.3961318580317652 1104 96-97 0.4049603406883584 1104 98-99 0.47056364966852726 1104 100-101 0.379161530715848 1105 1 0.19716352338941334 1105 2 0.43174687152973945 1105 3 0.49317836596721776 1105 4 0.3288256625170618 1105 5 0.2646756764757683 1105 6 0.27730068206843583 1105 7 0.2234669746942899 1105 8 0.2916488272412394 1105 9 0.3806964655939922 1105 10-11 0.47912578157598773 1105 12-13 0.5363447198639122 1105 14-15 0.49881433356388527 1105 16-17 0.6656443899043438 1105 18-19 0.44402782254721984 1105 20-21 0.4688708638036658 1105 22-23 0.5779161261855492 1105 24-25 0.6901594438375298 1105 26-27 0.6432270450467641 1105 28-29 0.6035027369155515 1105 30-31 0.6458409351349772 1105 32-33 0.6947035781724153 1105 34-35 0.592402034180445 1105 36-37 0.5684563674012963 1105 38-39 0.9706336059278584 1105 40-41 0.779310083118574 1105 42-43 0.687825980266858 1105 44-45 0.6695756949304226 1105 46-47 0.9665066630662764 1105 48-49 0.9497417626722893 1105 50-51 0.8209199469878499 1105 52-53 0.8454370222591905 1105 54-55 0.9023232641545675 1105 56-57 0.9050178867465348 1105 58-59 1.048149343308019 1105 60-61 1.103741242658323 1105 62-63 1.0998605188450057 1105 64-65 0.9782528491195848 1105 66-67 0.9170700986716582 1105 68-69 1.0584998406995396 1105 70-71 1.1186715357035624 1105 72-73 1.1179973360517472 1105 74-75 1.1906591762113656 1105 76-77 1.2896782932368396 1105 78-79 1.2740550525181327 1105 80-81 1.20226080261995 1105 82-83 1.0695579804104192 1105 84-85 1.1188408277210513 1105 86-87 1.2814982455222186 1105 88-89 1.0769087961234511 1105 90-91 1.166210873504916 1105 92-93 1.1153399136633517 1105 94-95 1.2558742090784634 1105 96-97 1.3015625951166996 1105 98-99 1.3588084161741634 1105 100-101 1.1422613696852544 1106 1 0.13894488637667735 1106 2 0.20435799783241038 1106 3 0.24651595293892825 1106 4 0.21271044968322883 1106 5 0.22405798741673522 1106 6 0.19057203381395027 1106 7 0.1785288043664579 1106 8 0.04721805520587452 1106 9 0.0736870772064222 1106 10-11 0.38771281405556124 1106 12-13 0.3961546733777226 1106 14-15 0.3282350791659283 1106 16-17 0.37863241895061606 1106 18-19 0.4097374205783879 1106 20-21 0.37339643120863997 1106 22-23 0.3950605675916776 1106 24-25 0.4933018191907337 1106 26-27 0.4009853776205148 1106 28-29 0.3853062795886615 1106 30-31 0.4053235104091826 1106 32-33 0.31612824319838495 1106 34-35 0.42415012763895277 1106 36-37 0.38232170936343834 1106 38-39 0.9369273196580039 1106 40-41 0.8664975166554783 1106 42-43 0.7544782149461611 1106 44-45 0.5117771047077113 1106 46-47 0.3315926473278168 1106 48-49 0.3814450410121495 1106 50-51 0.2441995021788088 1106 52-53 0.4630493638871158 1106 54-55 0.39701609069727084 1106 56-57 0.40556554268795253 1106 58-59 0.5237159887064919 1106 60-61 0.5179962482792035 1106 62-63 0.4879085545755686 1106 64-65 0.48868109935505544 1106 66-67 0.4046327638799312 1106 68-69 0.40018245851013035 1106 70-71 0.33468952251728723 1106 72-73 0.30764428406621036 1106 74-75 0.4009826046439038 1106 76-77 0.5893296468053393 1106 78-79 0.664997081503639 1106 80-81 0.725057266023466 1106 82-83 0.6727626411827607 1106 84-85 0.7508980695403906 1106 86-87 1.007270736635153 1106 88-89 0.8989236534746503 1106 90-91 0.8490930173386637 1106 92-93 0.8106320474706585 1106 94-95 0.9217083779479083 1106 96-97 1.0074690758386033 1106 98-99 1.0472932094169565 1106 100-101 0.9306360240771383 1107 1 0.15766903618325045 1107 2 0.14256591518631012 1107 3 0.16976508969352722 1107 4 0.20476749337880307 1107 5 0.22883360093117489 1107 6 0.21660051267261338 1107 7 0.3127618522042752 1107 8 0.2533609137816981 1107 9 0.33387585675964715 1107 10-11 0.4135287356013748 1107 12-13 0.31303521091367514 1107 14-15 0.34574168157818974 1107 16-17 0.37329221500068144 1107 18-19 0.3468292926255785 1107 20-21 0.34815751917727056 1107 22-23 0.2590936252692231 1107 24-25 0.34703062376362936 1107 26-27 0.44016900468894704 1107 28-29 0.41436883496700005 1107 30-31 0.28034864710263463 1107 32-33 0.3410382884659313 1107 34-35 0.38681073379509456 1107 36-37 0.28145180064844055 1107 38-39 0.8143820580859256 1107 40-41 0.4389473184995225 1107 42-43 0.35070625407390565 1107 44-45 0.2916360335239432 1107 46-47 0.3876378011882622 1107 48-49 0.1898365648952307 1107 50-51 0.14868699306153843 1107 52-53 0.20631476008200167 1107 54-55 0.3211933147751864 1107 56-57 0.22473491647500765 1107 58-59 0.3311560200789998 1107 60-61 0.3046152161295481 1107 62-63 0.2493268229451715 1107 64-65 0.3287761435215941 1107 66-67 0.3238662392206564 1107 68-69 0.4560668808295034 1107 70-71 0.46229938001790316 1107 72-73 0.4162890138628761 1107 74-75 0.4534517802511111 1107 76-77 0.4482028160062157 1107 78-79 0.28153843488709995 1107 80-81 0.27543979753807335 1107 82-83 0.2732413489107195 1107 84-85 0.2626004243410591 1107 86-87 0.19645510407175593 1107 88-89 0.33730806504674504 1107 90-91 0.414913658134477 1107 92-93 0.46788481346225197 1107 94-95 0.5337764746237497 1107 96-97 0.8103295718470633 1107 98-99 0.8875353719034109 1107 100-101 0.9134355997267605 1108 1 0.03718652930105293 1108 2 0.014578345946581805 1108 3 0.07509591355644574 1108 4 0.0782815775602117 1108 5 0.0731281649000124 1108 6 0.05931238887411183 1108 7 0.10986970434085208 1108 8 0.1769148512955212 1108 9 0.305491402351997 1108 10-11 0.3389782816487923 1108 12-13 0.3093028238661688 1108 14-15 0.42162828660076457 1108 16-17 0.3050308503914465 1108 18-19 0.33055237674700777 1108 20-21 0.40480716557910057 1108 22-23 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2316 72-73 -1.361215296437976 2316 74-75 -1.6061757485437376 2316 76-77 -1.4330751754445927 2316 78-79 -1.638574347067788 2316 80-81 -1.4850758062611114 2316 82-83 -1.4416654057642972 2316 84-85 -1.302471090946483 2316 86-87 -1.2975143004436056 2316 88-89 -1.652705554483763 2316 90-91 -1.5665762776955994 2316 92-93 -1.8863619805920813 2316 94-95 -1.7258337109487307 2316 96-97 -1.6271710657714067 2316 98-99 -1.9365061600132236 2316 100-101 -1.5866893734932646 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5221.0 3 965.0 4 940.0 5 1058.0 6 1351.0 7 1586.0 8 1995.0 9 2568.0 10 3036.0 11 4190.0 12 6032.0 13 8049.0 14 8905.0 15 10488.0 16 13836.0 17 18717.0 18 15912.0 19 9851.0 20 9195.0 21 9781.0 22 10510.0 23 11537.0 24 12913.0 25 14834.0 26 17295.0 27 20069.0 28 23534.0 29 28015.0 30 33452.0 31 40792.0 32 51030.0 33 65225.0 34 89117.0 35 130166.0 36 194302.0 37 296905.0 38 273048.0 39 36610.0 40 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.222229511256714 19.53749047259319 15.206045600350054 20.034234415800043 2 14.492435424977455 22.716829194969783 39.26796396566808 23.52277141438468 3 21.70146043292804 26.507350805204 30.48006885342031 21.31111990844765 4 11.805768588853919 16.78216637634076 39.97929654190086 31.432768492904472 5 11.994417994870773 40.03642494872462 33.67814266550882 14.29101439089579 6 28.291718731330135 37.63547782365994 17.71562196430102 16.357181480708903 7 25.952250257748144 35.25906810965116 21.18416281985582 17.604518812744878 8 25.249507063176946 31.438902844177946 20.679712611079598 22.631877481565514 9 25.79100941952013 18.730724895854507 22.51190354800349 32.96636213662187 10-11 24.573507427918603 26.602191441925033 26.97557922370452 21.848721906451843 12-13 28.10775247756704 24.930005650857993 26.66583850428473 20.296403367290242 14-15 23.58231842672905 25.98166533926685 29.043958147573324 21.39205808643078 16-17 20.132327343094182 29.358990067348422 28.994114396792337 21.514568192765065 18-19 19.626873457249676 29.39074635099771 31.17274853410232 19.809631657650304 20-21 22.470252267257806 26.322243408286074 29.72608513285418 21.481419191601944 22-23 20.823391580606128 26.867571969846644 30.391959194825258 21.917077254721974 24-25 22.13483237160583 26.798079112305583 30.5229068154625 20.544181700626083 26-27 21.624180190975522 25.62674292268271 32.5794191839449 20.16965770239687 28-29 19.921437668378612 28.66488278636564 31.173629102173244 20.2400504430825 30-31 20.54900501410216 27.038802558921105 30.731367315402153 21.680825111574578 32-33 20.553393323453562 27.369861921854334 30.879432557046226 21.197312197645875 34-35 22.21219604702536 26.54140601712615 30.456892465380093 20.789505470468402 36-37 21.60691064525122 25.95116386124377 31.892531634893178 20.549393858611833 38-39 21.10365434307732 27.13439214999741 31.406308676334692 20.355644830590574 40-41 20.920920581993315 27.688394128059656 30.992613349332775 20.398071940614255 42-43 20.187694054153642 28.194747736373536 29.318907173704545 22.298651035768277 44-45 21.036173688162346 26.9241307277377 29.49282192293012 22.546873661169837 46-47 22.232586053669745 27.875929357957258 29.055644969003335 20.83583961936966 48-49 21.679001974778387 27.473774062318995 29.30577902128222 21.5414449416204 50-51 20.122509047616628 28.252747308577497 30.642435819924906 20.982307823880966 52-53 21.25102125705057 29.22576860178224 28.128095103250274 21.39511503791692 54-55 19.964913944336445 27.593760371722535 29.471169151364496 22.97015653257652 56-57 21.73764082716557 27.65900037849575 28.335248381913726 22.268110412424953 58-59 23.01988359205902 27.06369533264683 28.234003386486684 21.682417688807465 60-61 21.41172322621009 27.575767726733037 30.361770614347183 20.65073843270969 62-63 21.15679104970523 27.65550984739043 29.686499193003435 21.501199909900908 64-65 20.530064215618697 29.221273426304116 28.96856350958221 21.28009884849497 66-67 21.045346872119648 27.664745668438616 28.11164620842509 23.178261251016647 68-69 20.3223621870652 28.004016569024788 28.966556710153522 22.70706453375649 70-71 21.90259161296267 27.777521829386608 28.0236613748584 22.296225182792316 72-73 21.29788456353287 26.453253230467816 29.894241725842797 22.35462048015652 74-75 20.24279813365618 29.425165019128873 28.357003633367906 21.97503321384704 76-77 20.83489543487044 27.195366493573964 28.51589685964251 23.453841211913083 78-79 20.741280029527942 27.418387577946824 29.262360169785666 22.57797222273956 80-81 21.592300115096 27.50211889731331 28.553017468924253 22.352563518666443 82-83 20.919617615065654 26.95991243672185 29.644958270625253 22.475511677587242 84-85 20.624511904286166 27.549561399646844 28.999404633294073 22.82652206277292 86-87 20.688052934755774 27.90961833426071 28.721568616826183 22.68076011415733 88-89 20.526400996010942 27.42139242384113 28.017109116168985 24.035097463978936 90-91 20.661678026043006 26.282536821994285 28.80330212327746 24.252483028685248 92-93 21.480356775658112 27.474971060844894 28.758490766741097 22.286181396755904 94-95 20.757478851081533 26.881654134727047 28.915243075363726 23.44562393882769 96-97 19.190448170532378 26.85439157934389 30.052381808949185 23.902778441174547 98-99 20.42086200799164 27.425201992897218 27.943920294950956 24.210015704160178 100-101 20.220686104836062 25.79172325501844 29.260586396442466 24.72700424370303 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3196.0 1 1974.0 2 870.5 3 998.5 4 874.0 5 920.5 6 1583.0 7 3171.0 8 4683.5 9 4828.5 10 3975.0 11 3337.5 12 3294.5 13 3311.5 14 3372.0 15 3485.0 16 3560.0 17 3563.0 18 3595.5 19 3771.5 20 4001.5 21 4324.5 22 4812.5 23 5527.0 24 6249.0 25 7111.0 26 8428.0 27 9960.5 28 11313.5 29 13190.5 30 15995.0 31 18821.0 32 22576.0 33 26554.0 34 30090.0 35 32734.5 36 35627.5 37 39527.0 38 40737.0 39 40300.0 40 40910.5 41 42443.0 42 43842.5 43 45370.0 44 47268.5 45 49373.0 46 54223.5 47 74293.0 48 120089.5 49 117607.5 50 70972.0 51 49534.5 52 40318.0 53 36426.5 54 33560.5 55 30127.0 56 26425.5 57 23019.0 58 20063.0 59 17301.5 60 14813.5 61 12616.5 62 10323.0 63 7278.0 64 4806.5 65 3605.5 66 2754.5 67 2101.5 68 1572.0 69 1135.5 70 841.5 71 678.0 72 577.0 73 533.0 74 433.0 75 332.5 76 257.5 77 163.5 78 110.0 79 83.0 80 58.5 81 38.0 82 28.0 83 23.5 84 16.5 85 11.0 86 7.0 87 4.5 88 4.0 89 4.0 90 3.0 91 1.5 92 1.0 93 1.0 94 1.5 95 1.0 96 0.0 97 1.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2972279181166754 2 0.1875880372505878 3 0.1881949000598097 4 0.20842366036720594 5 0.22130263776291487 6 0.11503421694805996 7 0.13081264998782902 8 0.14274761856919282 9 0.1467259414296474 10-11 0.13506068965238224 12-13 0.12447430509151154 14-15 0.13967958992257107 16-17 0.11533764835267091 18-19 0.1428487623707298 20-21 0.16884271936573397 22-23 0.17116902680108453 24-25 0.19473553255920115 26-27 0.17433819924924326 28-29 0.1723827524195283 30-31 0.16270666207249043 32-33 0.1733604758343858 34-35 0.35322786956765073 36-37 0.46779008210853806 38-39 0.4350194904105562 40-41 0.42608512127478954 42-43 0.4480670408088267 44-45 0.4518767906667197 46-47 0.4550796777153908 48-49 0.46674492949265595 50-51 0.4598334363876289 52-53 0.46627292508548335 54-55 0.4495504832313691 56-57 0.4138804358893271 58-59 0.4039009141376783 60-61 0.3605102232783134 62-63 0.46421633445423144 64-65 0.4611145912070973 66-67 0.47075696695362285 68-69 0.4821525019267894 70-71 0.47503872121868845 72-73 0.4676215091059765 74-75 0.47173469036848037 76-77 0.4491121934247089 78-79 0.4362332160289999 80-81 0.4344800568023589 82-83 0.4463138815821857 84-85 0.44746017799960486 86-87 0.44864018901753633 88-89 0.45501224851436617 90-91 0.4731507035899981 92-93 0.4784438958704334 94-95 0.47706159724942804 96-97 0.47520729422125 98-99 0.4716672611674557 100-101 0.45393338129797167 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1483037.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.05287672519146 #Duplication Level Percentage of deduplicated Percentage of total 1 68.68142799893725 28.19570196950484 2 14.901151345447468 12.234702584961516 3 6.2413197115771455 7.686723861656522 4 3.3049301250935024 5.427075560433407 5 1.8939257477903724 3.8875550125352105 6 1.1941344448273132 2.9413592494080385 7 0.8063985366202157 2.317348580067115 8 0.5654848672833285 1.857182443723499 9 0.408814933746681 1.5104726170667826 >10 1.8342129153264775 12.898831107797623 >50 0.10450526923258434 2.933895471883844 >100 0.05332532376242437 3.904196249314758 >500 0.0032916763032270705 1.0313333883745326 >1k 0.004772930639679252 4.579987796840127 >5k 0.0016458381516135353 4.4817462646928155 >10k+ 6.58335260645414E-4 4.111887841739318 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 24224 1.633404965621222 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 14643 0.9873657906040105 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 11142 0.7512961578166965 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10848 0.7314719727154481 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 9172 0.6184606317981277 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7672 0.5173168302611466 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 7088 0.47793817686274853 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 6837 0.46101344740556033 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 6490 0.43761551465000537 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6044 0.40754209099300964 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 6031 0.4066655113796891 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 5861 0.3952025472054979 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 5742 0.38717847228356406 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 5394 0.3637131103269844 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 4809 0.3242670277275617 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 4567 0.3079491610795954 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 3794 0.2558263886875378 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 3631 0.24483542892051918 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 3585 0.2417336856733851 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 3511 0.23674392479756068 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 3438 0.2318215931227609 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 3072 0.20714250554773753 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 2851 0.19224065212128894 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 2703 0.18226113036964015 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 2628 0.17720394029279107 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 2237 0.1508391226921513 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2136 0.14402877338866124 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 2118 0.14281504777021747 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 2077 0.14005045052820664 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 2063 0.1391064417138615 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1989 0.13411668083803707 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 1817 0.12251885826179656 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1804 0.12164227864847607 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1724 0.11624794256650374 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1577 0.10633585001587958 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 6.742920102465414E-5 0.0 10-11 0.0 0.0 0.0 6.742920102465414E-5 0.0 12-13 0.0 0.0 0.0 1.011438015369812E-4 0.0 14-15 0.0 0.0 0.0 1.3485840204930828E-4 0.0 16-17 0.0 0.0 0.0 1.3485840204930828E-4 0.0 18-19 0.0 0.0 0.0 1.3485840204930828E-4 0.0 20-21 0.0 0.0 0.0 2.6971680409861655E-4 0.0 22-23 0.0 0.0 0.0 6.068628092218873E-4 0.0 24-25 0.0 0.0 0.0 0.0012137256184437746 0.0 26-27 0.0 0.0 0.0 0.0026971680409861656 0.0 28-29 0.0 0.0 0.0 0.007248639110150321 0.0 30-31 0.0 0.0 0.0 0.021611058928401653 0.0 32-33 0.0 0.0 0.0 0.048279307933652364 0.0 34-35 0.0 0.0 0.0 0.09059113157662284 0.0 36-37 0.0 0.0 0.0 0.15491858935414288 0.0 38-39 0.0 0.0 0.0 0.2539720856593598 0.0 40-41 0.0 0.0 0.0 0.3638479687290337 0.0 42-43 0.0 0.0 0.0 0.4764210198396938 0.0 44-45 0.0 0.0 0.0 0.609930837868509 0.0 46-47 0.0 0.0 0.0 0.7771552564096513 0.0 48-49 0.0 0.0 0.0 0.943401951535936 0.0 50-51 0.0 0.0 0.0 1.126067657111724 0.0 52-53 0.0 0.0 0.0 1.3116665329320845 0.0 54-55 0.0 0.0 0.0 1.5205959123069754 0.0 56-57 0.0 0.0 0.0 1.7870424001558964 0.0 58-59 0.0 0.0 0.0 2.0604678103108687 0.0 60-61 0.0 0.0 0.0 2.376811906918034 0.0 62-63 0.0 0.0 0.0 2.7005395010373983 0.0 64-65 0.0 0.0 0.0 3.016344164036366 0.0 66-67 0.0 0.0 0.0 3.386294475458131 0.0 68-69 0.0 0.0 0.0 3.8231682688968647 0.0 70-71 0.0 0.0 0.0 4.294498384059198 0.0 72-73 0.0 0.0 0.0 4.769537105277886 0.0 74-75 0.0 0.0 0.0 5.288741953167722 0.0 76-77 0.0 0.0 0.0 5.801911887565852 0.0 78-79 0.0 0.0 0.0 6.4513899518353215 0.0 80-81 0.0 0.0 0.0 7.152855930094798 0.0 82-83 0.0 0.0 0.0 7.814639823551267 0.0 84-85 0.0 0.0 0.0 8.550292406730243 0.0 86-87 0.0 0.0 0.0 9.30853377225248 0.0 88-89 0.0 0.0 0.0 10.109424107422809 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACACG 160 6.289156E-8 26.69905 1 TAGCCGC 90 9.040754E-4 26.342703 5 GTACATG 10850 0.0 25.329237 1 TACATGG 11085 0.0 24.554083 2 GAGTACT 8020 0.0 23.546305 12-13 ACATGGG 11340 0.0 23.500158 3 AGTACTT 8230 0.0 23.233387 12-13 ATGGGAG 2835 0.0 22.077694 5 GTCGGGA 500 0.0 21.812496 2 CATGGGA 8370 0.0 21.697323 4 TGTACCG 155 3.960535E-5 21.414068 5 GTACTTT 8720 0.0 21.361555 14-15 CATGGGG 2325 0.0 21.006182 4 GTAAGCG 265 0.0 19.734816 94-95 CATTCCG 265 1.9068466E-8 19.67051 9 TACTTTT 9640 0.0 19.568739 14-15 TACACGA 245 1.7889215E-7 19.354477 2 ACTTTTT 9860 0.0 19.185043 16-17 GTAGCCC 670 0.0 19.108934 3 GCAACGA 125 0.006111135 18.95714 8 >>END_MODULE