##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939296_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1510805 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.922638593332692 33.0 31.0 34.0 30.0 34.0 2 31.960685197626432 33.0 31.0 34.0 30.0 34.0 3 32.013122143493035 34.0 31.0 34.0 30.0 34.0 4 35.50959455389676 37.0 35.0 37.0 33.0 37.0 5 35.579474518551365 37.0 35.0 37.0 33.0 37.0 6 35.70642604439355 37.0 35.0 37.0 33.0 37.0 7 35.63945313922048 37.0 35.0 37.0 33.0 37.0 8 35.63603310817743 37.0 35.0 37.0 33.0 37.0 9 37.23696439977363 39.0 37.0 39.0 34.0 39.0 10-11 37.15456428857463 39.0 37.0 39.0 33.0 39.0 12-13 37.072842292685024 39.0 37.0 39.0 33.0 39.0 14-15 38.199752780802285 40.0 38.0 41.0 33.0 41.0 16-17 38.19469653595269 40.0 38.0 41.0 33.0 41.0 18-19 38.2334460767604 40.0 38.0 41.0 33.5 41.0 20-21 38.15146163800093 40.0 38.0 41.0 33.0 41.0 22-23 38.08011722227555 40.0 38.0 41.0 33.0 41.0 24-25 38.07631990892273 40.0 38.0 41.0 33.0 41.0 26-27 37.9698862526931 40.0 38.0 41.0 33.0 41.0 28-29 37.85402252441579 40.0 38.0 41.0 33.0 41.0 30-31 37.6876317592277 40.0 37.0 41.0 33.0 41.0 32-33 37.43800324992306 40.0 36.5 41.0 32.5 41.0 34-35 37.09865932400277 40.0 36.0 41.0 31.0 41.0 36-37 36.81188571655508 39.5 35.5 41.0 30.5 41.0 38-39 36.47883346957417 39.0 35.0 41.0 30.0 41.0 40-41 36.39180966438422 39.0 35.0 41.0 30.0 41.0 42-43 36.181342396934085 39.0 35.0 41.0 30.0 41.0 44-45 36.017345388716606 39.0 35.0 41.0 29.5 41.0 46-47 35.70148695562962 39.0 35.0 41.0 27.5 41.0 48-49 35.40305598670907 39.0 35.0 41.0 26.0 41.0 50-51 34.210385522949686 38.0 34.0 40.0 23.5 40.5 52-53 34.35059653628364 38.0 34.5 39.5 23.5 40.5 54-55 34.89119244376342 39.0 35.0 40.5 24.0 41.0 56-57 34.78223629124871 39.0 35.0 41.0 23.5 41.0 58-59 34.58970681193139 38.5 35.0 40.5 23.0 41.0 60-61 34.24232544901559 38.0 34.0 40.0 22.0 41.0 62-63 33.922387402742245 38.0 34.0 40.0 22.0 41.0 64-65 33.55066173331436 37.0 33.5 40.0 20.0 41.0 66-67 33.13083687173395 37.0 33.0 39.5 18.5 41.0 68-69 32.69320031374002 36.0 33.0 39.0 16.5 41.0 70-71 32.194102481789514 35.5 32.0 38.5 12.0 40.0 72-73 31.728271021078168 35.0 31.5 37.5 8.0 40.0 74-75 31.288933383196373 35.0 31.0 37.0 7.0 39.0 76-77 30.841725437763312 35.0 31.0 36.5 2.0 39.0 78-79 30.391901337366505 35.0 31.0 36.0 2.0 38.5 80-81 29.887021819493583 34.5 30.0 36.0 2.0 37.0 82-83 29.429679541701276 34.0 29.5 35.0 2.0 37.0 84-85 28.98611170865863 34.0 29.0 35.0 2.0 36.5 86-87 28.716468041871718 34.0 29.0 35.0 2.0 36.0 88-89 28.27835557864847 34.0 29.0 35.0 2.0 36.0 90-91 27.82824785462055 34.0 27.5 35.0 2.0 35.0 92-93 27.40055334738765 34.0 26.5 35.0 2.0 35.0 94-95 26.891445620050238 34.0 25.5 35.0 2.0 35.0 96-97 26.308993880745696 33.0 25.0 35.0 2.0 35.0 98-99 25.61809432719643 33.0 22.0 35.0 2.0 35.0 100-101 23.243969274658212 31.0 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.27946715598444527 1101 2 0.14953936145613866 1101 3 0.29949359637593886 1101 4 0.35236782530768807 1101 5 0.31790493230514016 1101 6 0.23393337556645832 1101 7 0.29646544228258165 1101 8 0.16719333388787305 1101 9 0.25157937793368035 1101 10-11 0.37867720487599144 1101 12-13 0.39334431226620126 1101 14-15 0.3969040768690135 1101 16-17 0.47104503333639514 1101 18-19 0.32985452876385324 1101 20-21 0.5294753475619203 1101 22-23 0.5026868603089127 1101 24-25 0.47477995798229955 1101 26-27 0.41189428300882724 1101 28-29 0.2898003547879071 1101 30-31 0.4767667432220506 1101 32-33 0.44705257978395707 1101 34-35 0.4422675194096115 1101 36-37 0.48686281745890625 1101 38-39 0.6081767629012802 1101 40-41 0.6584370423620314 1101 42-43 0.5558804130016881 1101 44-45 0.6397485808337535 1101 46-47 0.8166784224843084 1101 48-49 0.8117250353475143 1101 50-51 0.8731299838466029 1101 52-53 0.7806869911472489 1101 54-55 0.8338458092859256 1101 56-57 0.7670625263646471 1101 58-59 0.7789385029967306 1101 60-61 0.7368937668571718 1101 62-63 0.8287069227356767 1101 64-65 0.5526344402002792 1101 66-67 0.6286383051062927 1101 68-69 0.6245046168140647 1101 70-71 0.6293841167162597 1101 72-73 0.6927532772610938 1101 74-75 0.4261689686735046 1101 76-77 0.379693396612911 1101 78-79 0.44828791450030536 1101 80-81 0.4839697655237565 1101 82-83 0.28873735734969586 1101 84-85 0.44923500703895414 1101 86-87 0.4631608654079997 1101 88-89 0.3214781185502389 1101 90-91 0.5489275911474536 1101 92-93 0.574737902766163 1101 94-95 0.7231534999410094 1101 96-97 0.5023219405928501 1101 98-99 0.07726675965231067 1101 100-101 0.22052377857727734 1102 1 0.26179133645361574 1102 2 0.3286164712668942 1102 3 0.36233098426751553 1102 4 0.286445832458071 1102 5 0.322166705164868 1102 6 0.34998182246047804 1102 7 0.4294204761217273 1102 8 0.40205663128038793 1102 9 0.43059863295287926 1102 10-11 0.5101253575370706 1102 12-13 0.5141858515342577 1102 14-15 0.6575735630712387 1102 16-17 0.5988244247512213 1102 18-19 0.5507571107405553 1102 20-21 0.5202720080416725 1102 22-23 0.5446557887437109 1102 24-25 0.5016207439573535 1102 26-27 0.45232887875733496 1102 28-29 0.5468111157791284 1102 30-31 0.6476207980164403 1102 32-33 0.6553115283852904 1102 34-35 0.5548919072114629 1102 36-37 0.648973419290428 1102 38-39 0.8380179101736047 1102 40-41 0.8831784078295755 1102 42-43 0.7732485394982334 1102 44-45 0.8945384262551954 1102 46-47 0.9393813511174685 1102 48-49 0.955524890828606 1102 50-51 0.8301323617966148 1102 52-53 0.9072304743362949 1102 54-55 0.8917985997760098 1102 56-57 0.8551012843521519 1102 58-59 0.7662538399477583 1102 60-61 0.7111519267442006 1102 62-63 0.6950726854507465 1102 64-65 0.6713262016779638 1102 66-67 0.6666642523899498 1102 68-69 0.6666527179961932 1102 70-71 0.6234138564405427 1102 72-73 0.591764786822484 1102 74-75 0.6873638708398744 1102 76-77 0.5747425613786383 1102 78-79 0.5959858841513359 1102 80-81 0.731094441418854 1102 82-83 0.6200053336599325 1102 84-85 0.6277232377213586 1102 86-87 0.5340368223734799 1102 88-89 0.5360069325978571 1102 90-91 0.6056052148032158 1102 92-93 0.5594964121433605 1102 94-95 0.41905926983479347 1102 96-97 0.3125647181895843 1102 98-99 0.3078207606063259 1102 100-101 0.1676731313120392 1103 1 0.30923905505728655 1103 2 0.41252427773456546 1103 3 0.32396428229318275 1103 4 0.43153314658634656 1103 5 0.3971625087616175 1103 6 0.22890777475461022 1103 7 0.2667777260595088 1103 8 0.2196334833412621 1103 9 0.29049722469783035 1103 10-11 0.36303360969170484 1103 12-13 0.3348026359896181 1103 14-15 0.4862497570203175 1103 16-17 0.5175681663222349 1103 18-19 0.45176974625543664 1103 20-21 0.5377961880065953 1103 22-23 0.5277306815183707 1103 24-25 0.7154535360993748 1103 26-27 0.5870614955186326 1103 28-29 0.5969760778378586 1103 30-31 0.6118156384663038 1103 32-33 0.637994274511577 1103 34-35 0.5719252526998915 1103 36-37 0.6731600405780611 1103 38-39 0.8676271526794039 1103 40-41 0.772509993143899 1103 42-43 0.7013103221303396 1103 44-45 0.7637677929745337 1103 46-47 0.6862019244908595 1103 48-49 0.6453797242169301 1103 50-51 0.6950400119844389 1103 52-53 0.6268705097597334 1103 54-55 0.5649288850735559 1103 56-57 0.5160217726398386 1103 58-59 0.518398925525446 1103 60-61 0.749811798131681 1103 62-63 0.6649268821434049 1103 64-65 0.7458381165691108 1103 66-67 0.6434821632178256 1103 68-69 0.6105181552614098 1103 70-71 0.40263818747597924 1103 72-73 0.5434728676481022 1103 74-75 0.4658536866032996 1103 76-77 0.4822361799516308 1103 78-79 0.40793434653130234 1103 80-81 0.36744513339128204 1103 82-83 0.4080238788682209 1103 84-85 0.22084981860774988 1103 86-87 0.34845942622722603 1103 88-89 0.14102756732555122 1103 90-91 0.19062231876403857 1103 92-93 0.18861250491571013 1103 94-95 0.11029312815264447 1103 96-97 0.09335358411884442 1103 98-99 0.23685610879203267 1103 100-101 0.292099381695504 1104 1 0.17149929750012305 1104 2 0.14119544544977103 1104 3 0.21778045537895707 1104 4 0.2237995268226527 1104 5 0.0688596086542077 1104 6 0.037462324394013535 1104 7 0.22428117856833296 1104 8 0.21851333603421352 1104 9 0.30651686563690816 1104 10-11 0.2595695925058905 1104 12-13 0.2908406903090892 1104 14-15 0.27205610963655147 1104 16-17 0.3355480650486484 1104 18-19 0.30423253588256927 1104 20-21 0.34127554967607665 1104 22-23 0.41082106052711964 1104 24-25 0.2699839948994338 1104 26-27 0.22236531629506118 1104 28-29 0.2182588766716762 1104 30-31 0.23676960501532562 1104 32-33 0.38029901595702853 1104 34-35 0.31211629152144127 1104 36-37 0.28969755784060425 1104 38-39 0.5315254187527501 1104 40-41 0.33812300526604133 1104 42-43 0.3306528110055851 1104 44-45 0.39869951002225434 1104 46-47 0.31753919624166116 1104 48-49 0.5211814734880633 1104 50-51 0.7763465947679222 1104 52-53 0.708188600905963 1104 54-55 0.4702035513310392 1104 56-57 0.6038497609780293 1104 58-59 0.6317025928570388 1104 60-61 0.6338803879736261 1104 62-63 0.5242870171748564 1104 64-65 0.6191387150039773 1104 66-67 0.6541619238438869 1104 68-69 0.6275620404846762 1104 70-71 0.5767405351819761 1104 72-73 0.5980033264481612 1104 74-75 0.6057800330817429 1104 76-77 0.49258219805811976 1104 78-79 0.2705973816701679 1104 80-81 0.3328011254121499 1104 82-83 0.4436844588897024 1104 84-85 0.4477448623395297 1104 86-87 0.5524298113737665 1104 88-89 0.48791263714295496 1104 90-91 0.49616551348553983 1104 92-93 0.5067803735669258 1104 94-95 0.5866012453844967 1104 96-97 0.5043095454507451 1104 98-99 0.19989124765960753 1104 100-101 0.25571377482117086 1105 1 0.12462039665510716 1105 2 0.20019737921104408 1105 3 0.18914428600347577 1105 4 0.2539378521092246 1105 5 0.29603741736840306 1105 6 0.20544672736109248 1105 7 0.286532846807674 1105 8 0.21255337377852612 1105 9 0.23874847692127332 1105 10-11 0.3306242117411031 1105 12-13 0.2394235860425411 1105 14-15 0.39434746864335324 1105 16-17 0.33092576205321933 1105 18-19 0.25133760639926805 1105 20-21 0.402143012979046 1105 22-23 0.4637456099861339 1105 24-25 0.461050292867931 1105 26-27 0.3886989345443581 1105 28-29 0.29728708758283773 1105 30-31 0.3157338156218188 1105 32-33 0.12011503359360631 1105 34-35 0.2108260242223139 1105 36-37 0.35049943405449113 1105 38-39 0.5712812443672561 1105 40-41 0.6119945899770514 1105 42-43 0.228488754422969 1105 44-45 0.4518779449873733 1105 46-47 0.4071803502359188 1105 48-49 0.36565755958910273 1105 50-51 0.23375254076648133 1105 52-53 0.10976988446645208 1105 54-55 0.2711070663758619 1105 56-57 0.300181009629263 1105 58-59 0.34103225049243235 1105 60-61 0.35198231877290453 1105 62-63 0.3132097809724783 1105 64-65 0.16034644835649203 1105 66-67 0.27874248383068334 1105 68-69 0.274065084995037 1105 70-71 0.2739265317037507 1105 72-73 0.3130173747794949 1105 74-75 0.17753019281803972 1105 76-77 0.17050674751713402 1105 78-79 0.21423638610047036 1105 80-81 0.2511531334349293 1105 82-83 0.16827792472306058 1105 84-85 0.27080835064175446 1105 86-87 0.18572657814184623 1105 88-89 0.12412009545283809 1105 90-91 0.05840607423399291 1105 92-93 0.09616076598928913 1105 94-95 0.06493317529517384 1105 96-97 -0.014136294423664708 1105 98-99 0.1726609692741512 1105 100-101 0.11266039962493934 1106 1 0.08197564860727269 1106 2 0.259466012236107 1106 3 0.18852754996650134 1106 4 0.21581616818994576 1106 5 0.21234923591006805 1106 6 0.15231546754029068 1106 7 0.26108373902580695 1106 8 0.17271142613211765 1106 9 0.2726799542489786 1106 10-11 0.413157887327948 1106 12-13 0.3742633746130366 1106 14-15 0.39687238797787217 1106 16-17 0.45095638894490975 1106 18-19 0.5251764033941484 1106 20-21 0.38730736263738663 1106 22-23 0.32822261382848694 1106 24-25 0.37433990491397395 1106 26-27 0.422275563747057 1106 28-29 0.3473991396245566 1106 30-31 0.16491636549047684 1106 32-33 0.4425485320562643 1106 34-35 0.3600686483791762 1106 36-37 0.32410972884832034 1106 38-39 0.4527469770336836 1106 40-41 0.5788947567056937 1106 42-43 0.26263563955613023 1106 44-45 0.39369384778097327 1106 46-47 0.40236940931863074 1106 48-49 0.4950156943074475 1106 50-51 0.426763432104984 1106 52-53 0.4194283506429315 1106 54-55 0.6941020245337768 1106 56-57 0.6849513279330282 1106 58-59 0.7748960567833407 1106 60-61 0.6202849852316561 1106 62-63 0.6353803919509815 1106 64-65 0.4388977284086266 1106 66-67 0.39116386747848253 1106 68-69 0.5113425302484629 1106 70-71 0.5400996096264024 1106 72-73 0.4285989058841899 1106 74-75 0.4476920635373709 1106 76-77 0.38381295246141534 1106 78-79 0.6827603578405679 1106 80-81 0.5455106484185173 1106 82-83 0.5783474149812307 1106 84-85 0.5468631991665909 1106 86-87 0.6300968876493762 1106 88-89 0.5705010444066829 1106 90-91 0.5452242160692897 1106 92-93 0.6921906651924346 1106 94-95 0.5350989443460215 1106 96-97 0.5044852355614964 1106 98-99 0.4305081106976232 1106 100-101 0.34464810888537656 1107 1 0.024674341708220027 1107 2 0.036197995725938625 1107 3 0.026756832081538562 1107 4 0.07250265681528845 1107 5 0.21396387227932934 1107 6 0.1429539521449925 1107 7 0.2015822322193941 1107 8 0.09102929758756062 1107 9 0.1971080335443105 1107 10-11 0.21748730690274698 1107 12-13 0.20538361532700122 1107 14-15 0.17422275281818145 1107 16-17 0.2634077482586932 1107 18-19 0.266593138978223 1107 20-21 0.319822219729879 1107 22-23 0.31679029447179374 1107 24-25 0.21808562687771627 1107 26-27 0.3299951571062323 1107 28-29 0.2154094144127825 1107 30-31 0.3239411701307162 1107 32-33 0.27486990470345773 1107 34-35 0.24868710286649076 1107 36-37 0.36351688913239144 1107 38-39 0.4072914939273531 1107 40-41 0.3551776049917592 1107 42-43 0.424212966760976 1107 44-45 0.5549451065784865 1107 46-47 0.41681018441909856 1107 48-49 0.5804327243141785 1107 50-51 0.40592716860982847 1107 52-53 0.39400251608596193 1107 54-55 0.6389770074216301 1107 56-57 0.6050765231990098 1107 58-59 0.4384030713563263 1107 60-61 0.39753056748528337 1107 62-63 0.4059695842014719 1107 64-65 0.39166797371708384 1107 66-67 0.24772728930738452 1107 68-69 0.3744960032169047 1107 70-71 0.321642215585733 1107 72-73 0.30546506734407686 1107 74-75 0.43594305229117225 1107 76-77 0.23253713656371744 1107 78-79 0.11890134878848002 1107 80-81 0.06668352890354612 1107 82-83 0.20809451154248393 1107 84-85 0.24892528296640393 1107 86-87 0.41296369233838703 1107 88-89 0.13005084027989255 1107 90-91 0.0757487666059049 1107 92-93 0.19410956956507874 1107 94-95 0.10205572047415856 1107 96-97 0.434808566025616 1107 98-99 0.6030268965510857 1107 100-101 0.5439061450299789 1108 1 0.059637064757986025 1108 2 0.140716506137462 1108 3 0.11043094929424058 1108 4 0.07663338885311788 1108 5 0.12917532933874298 1108 6 0.05973032813606238 1108 7 0.17129201378014614 1108 8 0.16625303421979254 1108 9 0.22536558305201737 1108 10-11 0.1786806325189545 1108 12-13 0.19257498547574414 1108 14-15 0.15956128801591518 1108 16-17 0.23993646197798313 1108 18-19 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-1.39288587250347 2316 88-89 -1.5381077710203144 2316 90-91 -1.5420480668774807 2316 92-93 -1.5708991775161785 2316 94-95 -1.5771977765284113 2316 96-97 -1.8253645822648572 2316 98-99 -1.6437809677703399 2316 100-101 -1.3031791371823367 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4221.0 3 927.0 4 919.0 5 967.0 6 1177.0 7 1424.0 8 1893.0 9 2417.0 10 3173.0 11 4304.0 12 6233.0 13 9097.0 14 8847.0 15 10425.0 16 14433.0 17 19676.0 18 15782.0 19 10004.0 20 9692.0 21 9731.0 22 10675.0 23 11613.0 24 13292.0 25 14960.0 26 17304.0 27 20532.0 28 24042.0 29 28167.0 30 34139.0 31 41636.0 32 51349.0 33 66280.0 34 89482.0 35 128731.0 36 193504.0 37 296476.0 38 288395.0 39 44873.0 40 13.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.193578384161945 19.4905213663357 15.204377541734729 20.11152270776763 2 14.480437080256179 22.753088751279734 39.2358384605445 23.530635707919593 3 21.74607445382985 26.52412718146647 30.517667111564105 21.212131253139578 4 11.784521156284193 16.811835086554307 39.98549972596603 31.41814403119547 5 12.002438967812916 40.113597576938496 33.655436147756376 14.228527307492204 6 28.336854514507486 37.444417216920215 17.832387360836076 16.386340907736223 7 25.914992180912684 35.3771144819035 21.121934709130613 17.585958628053202 8 25.236340850138173 31.43995697112773 20.691747412041195 22.631954766692896 9 25.847197733122613 18.686305342237283 22.45913540194285 33.00736152269725 10-11 24.558033161049362 26.575467764727236 26.95144135351817 21.91505772070524 12-13 28.084112412621632 24.924995437960735 26.671287766813062 20.319604382604574 14-15 23.601684320683265 25.966191054157566 29.098279085247626 21.333845539911543 16-17 20.11117995740508 29.31757058612555 29.05959016548169 21.511659290987676 18-19 19.623408860208215 29.36990284670578 31.215798877612023 19.790889415473984 20-21 22.418645750740396 26.317197944176947 29.825629958474174 21.43852634660848 22-23 20.8253991176832 26.878931018115683 30.46258533594648 21.83308452825464 24-25 22.13445517332759 26.848766914553558 30.49644732265598 20.520330589462876 26-27 21.64356126693403 25.66794541094663 32.512247225513576 20.17624609660576 28-29 19.955323383776705 28.67782985854061 31.17455976739541 20.192286990287275 30-31 20.54142426936341 27.01301824223258 30.753510593054727 21.692046895349286 32-33 20.57655761171172 27.403029939715186 30.89711174716013 21.123300701412962 34-35 22.17501487048167 26.545261168121538 30.487157974774753 20.79256598662204 36-37 21.602077431350995 25.94709897327513 31.930309857601753 20.520513737772124 38-39 21.13019575652765 27.152086537720788 31.381711946487727 20.336005759263834 40-41 20.875631147127336 27.692385331091618 30.995266894556927 20.436716627224122 42-43 20.184660425862024 28.199785006726426 29.337349817700716 22.278204749710834 44-45 21.033544425929882 26.92431986656229 29.528507464520732 22.513628242987096 46-47 22.218605505476948 27.836315076405537 29.064662798920253 20.88041661919726 48-49 21.678142348054628 27.49685758550955 29.265246357323754 21.559753709112073 50-51 20.13480378316866 28.21907807020399 30.66805996682387 20.97805817980348 52-53 21.254771127070505 29.25445357785951 28.099949610653447 21.39082568441654 54-55 19.959853255811947 27.579830180069177 29.55153084371266 22.908785720406225 56-57 21.75891737679835 27.632545370508105 28.35696601993327 22.25157123276027 58-59 22.95805930062315 27.10309384451431 28.284491602232322 21.654355252630218 60-61 21.43191363399845 27.601240854208925 30.345376397752975 20.62146911403965 62-63 21.143783947493215 27.605048770152642 29.723093394543426 21.528073887810717 64-65 20.51078143254384 29.224985193032037 29.014614967344947 21.249618407079176 66-67 21.080721411090778 27.68106616456281 28.115413018180778 23.12279940616563 68-69 20.37159594091037 28.02547961152662 28.923106670706623 22.679817776856385 70-71 21.933704153822948 27.769409103685106 28.019749480215633 22.277137262276312 72-73 21.320042466286736 26.54039944352688 29.872162658102543 22.267395432083838 74-75 20.21511784541984 29.47867906295044 28.34492204486195 21.961281046767766 76-77 20.830417394198324 27.187336315756433 28.56204315800277 23.42020313204247 78-79 20.772252082568194 27.40346267847857 29.30654779496491 22.51773744398833 80-81 21.582165242997768 27.480783458432896 28.567960874895565 22.36909042367377 82-83 20.93094621001885 26.984693680995697 29.632280365215518 22.452079743769936 84-85 20.643925743644655 27.521702609893072 28.95367463871054 22.880697007751728 86-87 20.69850547990701 27.846130853537034 28.737263367651945 22.718100298904016 88-89 20.550267158355847 27.460311555205084 28.047475331946664 23.9419459544924 90-91 20.622391116273324 26.298013095416874 28.81147862326689 24.26811716504292 92-93 21.41462041984397 27.46420874524879 28.84868321604187 22.272487618865373 94-95 20.813804375992508 26.862919676019747 28.952911237649747 23.370364710338 96-97 19.216871512760218 26.821238698137016 30.07646096118744 23.885428827915327 98-99 20.388524646801816 27.434881576289744 27.99779948761016 24.17879428929828 100-101 20.21685541710093 25.861732252709697 29.26292673108693 24.65848559910245 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2521.0 1 1594.5 2 823.0 3 946.5 4 802.5 5 860.5 6 1558.0 7 3259.5 8 4823.0 9 4956.5 10 4053.0 11 3321.0 12 3292.0 13 3359.5 14 3440.5 15 3514.5 16 3643.5 17 3688.5 18 3705.5 19 3869.5 20 4039.0 21 4454.5 22 5061.5 23 5717.5 24 6388.0 25 7260.0 26 8760.5 27 10402.5 28 11608.5 29 13419.5 30 16187.5 31 19171.5 32 23030.5 33 26987.0 34 30637.5 35 33134.5 36 35944.5 37 40065.0 38 41589.0 39 41415.5 40 41906.0 41 43243.0 42 44748.5 43 46185.5 44 48334.0 45 50670.0 46 54995.0 47 75494.0 48 122851.5 49 120022.5 50 71759.5 51 50160.5 52 41198.0 53 37335.5 54 34416.0 55 30790.5 56 26982.0 57 23451.5 58 20458.0 59 17633.5 60 15093.0 61 12950.5 62 10402.5 63 7169.0 64 4875.5 65 3749.0 66 2790.5 67 2163.5 68 1604.5 69 1108.5 70 863.0 71 678.0 72 545.0 73 527.5 74 438.0 75 340.5 76 264.5 77 175.0 78 118.0 79 77.5 80 50.0 81 32.0 82 32.5 83 26.5 84 13.5 85 8.0 86 4.5 87 3.5 88 3.5 89 2.5 90 2.5 91 2.0 92 1.5 93 2.5 94 3.0 95 1.5 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2783946306770232 2 0.11252279413954812 3 0.12198794682305128 4 0.1236426937956917 5 0.1303940614440646 6 0.0592399416205268 7 0.06870509430402998 8 0.07419885425319615 9 0.07035984127667039 10-11 0.0656272649349188 12-13 0.07039293621612319 14-15 0.0660244042083525 16-17 0.06622297384506935 18-19 0.07131959452080182 20-21 0.0755888417102141 22-23 0.08316758284490719 24-25 0.09134203288975083 26-27 0.09144131770810925 28-29 0.08541803872769815 30-31 0.08147974093281396 32-33 0.07532408219459162 34-35 0.23990521609340718 36-37 0.3630183908578539 38-39 0.33472221762570287 40-41 0.3188366466883549 42-43 0.342631908154924 44-45 0.35633321308838667 46-47 0.3607017450961573 48-49 0.3701999927191133 50-51 0.3644414732543247 52-53 0.3657983657718898 54-55 0.35265967480912497 56-57 0.32969178682887595 58-59 0.31440192480167856 60-61 0.27144469339193344 62-63 0.3422678638209431 64-65 0.3707626066898111 66-67 0.37539589821320424 68-69 0.3863503231720838 70-71 0.3874755511134792 72-73 0.37139141053941443 74-75 0.37572684760773234 76-77 0.359377947518045 78-79 0.3370388633873994 80-81 0.3356488759303815 82-83 0.34968113025837216 84-85 0.3573260612719709 86-87 0.35113730759429573 88-89 0.3652357518011921 90-91 0.38727698147676237 92-93 0.3834710634396894 94-95 0.3822465506799355 96-97 0.3874755511134792 98-99 0.37734849964091993 100-101 0.35596916875440576 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1510805.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.594989277088715 #Duplication Level Percentage of deduplicated Percentage of total 1 68.19197182154369 27.682523648791012 2 14.970863456641418 12.15484082982235 3 6.421503669369248 7.820426178024914 4 3.330227596699981 5.407622143132024 5 1.986314422539498 4.031720634195879 6 1.2261831899723135 2.9866136069203493 7 0.8307132355688202 2.3605956423176346 8 0.5829087653660188 1.8930540063643642 9 0.42092230854760443 1.5378602941779684 >10 1.8623429633338808 12.907654365260433 >50 0.11153662455701302 3.0551317476143636 >100 0.05337793990031163 3.791430857639221 >500 0.003929649162485645 1.1235147004460377 >1k 0.004912061453107057 4.673191033796343 >5k 0.0016373538177023523 4.448062696198831 >10k+ 6.549415270809409E-4 4.125757615298232 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 24624 1.6298595781719019 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 14824 0.9811987648968598 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 11507 0.7616469365669296 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11116 0.7357666939148335 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 9296 0.6153011143066114 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7898 0.5227676635965595 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 7167 0.4743828621165538 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 6847 0.4532021008667565 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 6519 0.4314918205857142 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6160 0.4077296540585979 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 6017 0.3982645013750947 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 5864 0.3881374499025354 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 5644 0.37357567654329976 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 5508 0.3645738530121359 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 4864 0.3219475709969189 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 4627 0.30626056969628773 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 3864 0.25575769209130234 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 3831 0.253573426087417 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 3634 0.2405340199430105 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 3607 0.23874689321255887 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 3589 0.23755547539225777 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 3297 0.21822803075181776 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 2998 0.19843725695903838 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 2683 0.17758744510376917 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 2570 0.17010798878743452 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 2343 0.15508288627585956 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 2156 0.14270537892050927 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2121 0.1403887331588127 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 2108 0.13952826473303967 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 2098 0.1388663659439835 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 2061 0.1364173404244757 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1837 0.12159080754961758 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 1831 0.12119366827618389 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1700 0.11252279413954812 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1581 0.10464619854977976 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 1515 0.10027766654200906 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 3.309493945280827E-5 0.0 12-13 0.0 0.0 0.0 6.618987890561654E-5 0.0 14-15 0.0 0.0 0.0 1.3237975781123308E-4 0.0 16-17 0.0 0.0 0.0 1.985696367168496E-4 0.0 18-19 0.0 0.0 0.0 2.6475951562246616E-4 0.0 20-21 0.0 0.0 0.0 4.302342128865075E-4 6.618987890561654E-5 22-23 0.0 0.0 0.0 0.0010590380624898646 6.618987890561654E-5 24-25 0.0 0.0 0.0 0.0022504558827909625 6.618987890561654E-5 26-27 0.0 0.0 0.0 0.0031440192480167856 6.618987890561654E-5 28-29 0.0 0.0 0.0 0.008273734863202068 6.618987890561654E-5 30-31 0.0 0.0 0.0 0.020551957400193936 6.618987890561654E-5 32-33 0.0 0.0 0.0 0.05020502314991015 6.618987890561654E-5 34-35 0.0 0.0 0.0 0.09213631143661823 6.618987890561654E-5 36-37 0.0 0.0 0.0 0.15531455085202922 6.618987890561654E-5 38-39 0.0 0.0 0.0 0.24811276107770358 6.618987890561654E-5 40-41 0.0 0.0 0.0 0.35980818173093154 6.618987890561654E-5 42-43 0.0 0.0 0.0 0.4733238240540639 6.618987890561654E-5 44-45 0.0 0.0 0.0 0.6063323857149003 6.618987890561654E-5 46-47 0.0 0.0 0.0 0.7695566270961507 6.618987890561654E-5 48-49 0.0 0.0 0.0 0.9362889320593988 6.618987890561654E-5 50-51 0.0 0.0 0.0 1.1185758585654668 6.618987890561654E-5 52-53 0.0 0.0 0.0 1.2996713672512334 6.618987890561654E-5 54-55 0.0 0.0 0.0 1.5068456882258134 6.618987890561654E-5 56-57 0.0 0.0 0.0 1.7693878429049414 6.618987890561654E-5 58-59 0.0 0.0 0.0 2.0419246692988176 6.618987890561654E-5 60-61 0.0 0.0 0.0 2.355830170008704 6.618987890561654E-5 62-63 0.0 0.0 0.0 2.666591651470574 6.618987890561654E-5 64-65 0.0 0.0 0.0 2.976029335354331 6.618987890561654E-5 66-67 0.0 0.0 0.0 3.3401398592141276 6.618987890561654E-5 68-69 0.0 0.0 0.0 3.7699769328272015 6.618987890561654E-5 70-71 0.0 0.0 0.0 4.241381250393003 6.618987890561654E-5 72-73 0.0 0.0 0.0 4.718113853210705 6.618987890561654E-5 74-75 0.0 0.0 0.0 5.2357187062526265 6.618987890561654E-5 76-77 0.0 0.0 0.0 5.747399565132495 6.618987890561654E-5 78-79 0.0 0.0 0.0 6.3984101191086875 6.618987890561654E-5 80-81 0.0 0.0 0.0 7.0958859680766215 6.618987890561654E-5 82-83 0.0 0.0 0.0 7.759836643378861 6.618987890561654E-5 84-85 0.0 0.0 0.0 8.483325114756703 6.618987890561654E-5 86-87 3.309493945280827E-5 0.0 0.0 9.243714443624427 6.618987890561654E-5 88-89 6.618987890561654E-5 0.0 0.0 10.034418737030922 6.618987890561654E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGTATC 90 2.4151934E-5 31.663334 1 GTACATG 11480 0.0 24.492197 1 TACATGG 11505 0.0 23.945919 2 AGTACTT 8595 0.0 23.430573 12-13 GAGTACT 8360 0.0 23.380701 12-13 ACATGGG 11695 0.0 22.624344 3 CATTCCG 210 3.5377525E-8 22.564232 9 GGACCGT 105 0.0022277285 22.564232 6 GTACTTT 8860 0.0 21.981392 14-15 TACGCAT 110 0.0029068214 21.553576 5 ATGGGAT 3195 0.0 21.519848 5 GTCTAGC 265 7.9489837E-10 21.507172 1 CATGGGA 8860 0.0 21.247059 4 GTATAGT 360 0.0 21.108889 1 CATGGGG 2360 0.0 20.494165 4 TACTTTT 9965 0.0 20.328524 14-15 ATGGGAG 2730 0.0 20.321943 5 CGTATCA 145 6.219317E-4 19.621185 2 ACTTTTT 10320 0.0 19.125427 16-17 CTACGCA 150 7.8129844E-4 18.967148 4 >>END_MODULE