FastQCFastQC Report
Fri 27 May 2016
SRR939295_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939295_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3105462
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT53190.1712788628551887No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51360.16538601985791487No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37130.11956353032173636No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA161550.023.765021
GTACATG128400.023.6913411
TACATGG129050.023.0018332
ACATGGG129600.021.6602173
ATCAACG185250.020.4519483
TATCAAC187550.020.3528352
GAGTACT62750.020.35234512-13
TCAACGC186800.020.2314764
CAACGCA188300.020.045135
AACGCAG191700.019.6784936
CATGGGG75900.019.4921114
CGATCGT3800.019.33871724-25
CGCGGAA3600.019.1439982-83
ATTCGCG3550.018.7434380-81
GTACTTT67700.018.51829114-15
AGAGTAC149600.018.48162510-11
GGTATCA120300.018.3435731
AGTACTT71200.018.136612-13
ACGCAGA208000.017.976897
CGCAGAG210300.017.6450718
Produced by FastQC (version 0.11.5)