FastQCFastQC Report
Fri 27 May 2016
SRR939295_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939295_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3105462
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT58330.18783034537212176No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51440.1656436304807465No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40710.13109160569345238No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG124350.023.0015281
TACATGG123900.022.3663462
TATACCG1503.0591982E-522.1442175
ACATGGG121200.021.5726013
GTATCAA175750.021.202861
AGTACTT74850.020.16057212-13
GAGTACT69950.019.46980912-13
CATGGGG73800.019.0321964
AGAGTAC151850.018.71886610-11
GTACTTT72800.018.34908914-15
ATCAACG201050.018.3387813
TATCAAC203400.018.2202192
CAACGCA203200.018.1914485
TCAACGC202700.018.18954
AACGCAG206450.017.8591046
GTAAGGT11150.017.02314
TACTTTT86150.017.02043714-15
GGTATCA137250.016.9560451
TAAGGTG12450.016.3890085
ACGCAGA225700.016.2728257
Produced by FastQC (version 0.11.5)