##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939295_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3105462 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.299979841968764 33.0 31.0 34.0 31.0 34.0 2 32.51148202747289 34.0 31.0 34.0 31.0 34.0 3 32.61114578120743 34.0 31.0 34.0 31.0 34.0 4 36.131783612229036 37.0 35.0 37.0 35.0 37.0 5 36.07045650534445 37.0 35.0 37.0 35.0 37.0 6 36.0962343123181 37.0 35.0 37.0 35.0 37.0 7 36.036997071611246 37.0 35.0 37.0 35.0 37.0 8 36.0040602654291 37.0 35.0 37.0 35.0 37.0 9 37.706919936550506 39.0 37.0 39.0 35.0 39.0 10-11 37.62622695109455 39.0 37.0 39.0 35.0 39.0 12-13 37.552322005550224 39.0 37.0 39.0 35.0 39.0 14-15 38.84920987601845 40.0 38.0 41.0 35.0 41.0 16-17 38.851698394635 40.0 38.0 41.0 35.0 41.0 18-19 38.93876933609234 40.0 38.0 41.0 35.0 41.0 20-21 38.907652387953874 40.0 38.0 41.0 35.0 41.0 22-23 38.83553477711207 40.0 38.0 41.0 34.5 41.0 24-25 38.76757001051695 40.0 38.0 41.0 34.0 41.0 26-27 38.61706341922716 40.0 38.0 41.0 34.0 41.0 28-29 38.45770790304309 40.0 38.0 41.0 34.0 41.0 30-31 38.29103447409757 40.0 38.0 41.0 34.0 41.0 32-33 38.28507899951762 40.0 38.0 41.0 34.0 41.0 34-35 38.211022707732376 40.0 38.0 41.0 33.5 41.0 36-37 38.07706582788648 40.0 38.0 41.0 33.0 41.0 38-39 37.95941618348574 40.0 38.0 41.0 33.0 41.0 40-41 37.794543935813735 40.0 37.0 41.0 33.0 41.0 42-43 37.6323497115727 40.0 37.0 41.0 33.0 41.0 44-45 37.41735529206282 40.0 37.0 41.0 32.0 41.0 46-47 37.162351527727594 40.0 36.5 41.0 31.0 41.0 48-49 36.891092211078416 39.5 36.0 41.0 31.0 41.0 50-51 36.65545513034776 39.0 36.0 41.0 30.5 41.0 52-53 36.39253418653972 39.0 35.0 40.5 30.0 41.0 54-55 36.138711727916814 39.0 35.0 40.0 29.5 41.0 56-57 35.80711356313489 38.0 35.0 40.0 29.0 41.0 58-59 35.47218771313254 38.0 34.5 40.0 28.0 41.0 60-61 35.10255607700239 38.0 34.0 40.0 28.0 41.0 62-63 34.7375917013314 37.0 34.0 40.0 27.0 41.0 64-65 34.40188818926137 37.0 34.0 39.5 26.5 41.0 66-67 33.9593463066043 36.0 33.0 39.0 26.0 40.5 68-69 33.47577832219489 35.5 33.0 39.0 26.0 40.0 70-71 32.97500323623345 35.0 32.0 38.0 25.0 40.0 72-73 32.47165301008352 35.0 32.0 37.0 24.0 39.0 74-75 31.945548037618877 35.0 31.0 37.0 22.5 39.0 76-77 30.020718817361153 33.0 29.0 35.0 20.0 37.0 78-79 30.977277132999856 34.0 30.5 35.5 20.0 37.0 80-81 30.994735887929075 34.0 31.0 35.0 20.5 37.0 82-83 30.63822242874007 34.0 31.0 35.0 19.5 37.0 84-85 30.191293276169535 34.0 30.0 35.0 18.0 36.0 86-87 29.588675372617665 34.0 29.5 35.0 10.0 36.0 88-89 28.99005784002509 34.0 29.0 35.0 6.0 35.0 90-91 28.49301939614782 33.0 29.0 35.0 2.0 35.0 92-93 28.115716276676388 33.0 28.0 35.0 2.0 35.0 94-95 27.571627667638502 33.0 27.0 35.0 2.0 35.0 96-97 26.804745960504427 33.0 25.5 34.0 2.0 35.0 98-99 25.7575714982183 32.0 24.0 34.0 2.0 35.0 100-101 21.389575850549775 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.24662037913614654 1101 2 0.288911829319467 1101 3 0.23761243087214012 1101 4 0.1647777500668326 1101 5 0.2195631073930855 1101 6 0.21107194262133788 1101 7 0.27405045540074013 1101 8 0.25080287870628126 1101 9 0.350294082424206 1101 10-11 0.3860527172557582 1101 12-13 0.30196515065112095 1101 14-15 0.30716802054644177 1101 16-17 0.328538320856957 1101 18-19 0.355816194780175 1101 20-21 0.38727862528449464 1101 22-23 0.3239956601764078 1101 24-25 0.3878358721642101 1101 26-27 0.34040123708147974 1101 28-29 0.41643542732154515 1101 30-31 0.44963421351294386 1101 32-33 0.4802462671130385 1101 34-35 0.4833001614258805 1101 36-37 0.4633832478674833 1101 38-39 0.48929190175777393 1101 40-41 0.502970686328915 1101 42-43 0.6298726557796854 1101 44-45 0.6664090225950474 1101 46-47 0.6834355227856008 1101 48-49 0.590831546471243 1101 50-51 0.6591032754489987 1101 52-53 0.5868179033426344 1101 54-55 0.6090414861149611 1101 56-57 0.5252572248584997 1101 58-59 0.4793291274011722 1101 60-61 0.5132250351132654 1101 62-63 0.5737250798249747 1101 64-65 0.5492527245141403 1101 66-67 0.44544032946893 1101 68-69 0.48652522374600693 1101 70-71 0.4525071059879906 1101 72-73 0.3235868410580096 1101 74-75 0.4167673961247509 1101 76-77 0.6271043412148423 1101 78-79 0.7741916127326469 1101 80-81 0.7272753396263703 1101 82-83 0.6602652688768025 1101 84-85 0.720335793786063 1101 86-87 0.6631735273102315 1101 88-89 0.6651094401928574 1101 90-91 0.7876844346814984 1101 92-93 0.6524538555852182 1101 94-95 0.5500764753761693 1101 96-97 0.5648247705259841 1101 98-99 0.28356524307156405 1101 100-101 0.3110386084067933 1102 1 0.20114571151128047 1102 2 0.22575001353314406 1102 3 0.22251457484147608 1102 4 0.13109108100466926 1102 5 0.18077367153250634 1102 6 0.1865508023650193 1102 7 0.14477665015460417 1102 8 0.20790852010046024 1102 9 0.2572850993124618 1102 10-11 0.20933927852058076 1102 12-13 0.2936900877693347 1102 14-15 0.3297428798110218 1102 16-17 0.3069251336489103 1102 18-19 0.26417755542217236 1102 20-21 0.3038697140376456 1102 22-23 0.23617424443800417 1102 24-25 0.3023413219186679 1102 26-27 0.3014107591799302 1102 28-29 0.2909139876281728 1102 30-31 0.3282153010683331 1102 32-33 0.3648693956314233 1102 34-35 0.30093185384413346 1102 36-37 0.35312165965331843 1102 38-39 0.3716411651426128 1102 40-41 0.32565771471554683 1102 42-43 0.33055243778951393 1102 44-45 0.35678188162007984 1102 46-47 0.386899111239309 1102 48-49 0.3847853732768556 1102 50-51 0.33540444924226875 1102 52-53 0.20780748293300633 1102 54-55 0.3323371502663619 1102 56-57 0.3075512720497855 1102 58-59 0.27877822310125566 1102 60-61 0.39510705691228054 1102 62-63 0.42654667522562306 1102 64-65 0.4188904655609704 1102 66-67 0.3748155839904328 1102 68-69 0.31773638675954174 1102 70-71 0.17045901639642125 1102 72-73 0.1830240790505826 1102 74-75 0.2524678392916009 1102 76-77 0.3285686483174892 1102 78-79 0.34855824346686504 1102 80-81 0.5473127692586601 1102 82-83 0.47540977720115407 1102 84-85 0.4660584551826368 1102 86-87 0.34893128273004237 1102 88-89 0.39313603020363885 1102 90-91 0.5953172050731617 1102 92-93 0.5460286549623916 1102 94-95 0.564442207799214 1102 96-97 0.3236043249636431 1102 98-99 0.0387468815911447 1102 100-101 -0.32296750010919695 1103 1 0.18856941703526786 1103 2 0.18771015557889825 1103 3 0.21258287045400692 1103 4 0.12224434274497042 1103 5 0.1560397935067428 1103 6 0.1567009301282738 1103 7 0.23135003396606635 1103 8 0.17103024346474172 1103 9 0.2382774050092067 1103 10-11 0.22235513038172883 1103 12-13 0.2266925978968004 1103 14-15 0.2959260630498548 1103 16-17 0.2635949670767914 1103 18-19 0.18436175021908952 1103 20-21 0.2658126325133807 1103 22-23 0.27271398883546993 1103 24-25 0.26470627725141327 1103 26-27 0.2521427371489722 1103 28-29 0.28448335989199336 1103 30-31 0.267057432913937 1103 32-33 0.2921932353329666 1103 34-35 0.3423735450514158 1103 36-37 0.3109490951611491 1103 38-39 0.23651406232288963 1103 40-41 0.3690005317028806 1103 42-43 0.3815775015285965 1103 44-45 0.45629764985782373 1103 46-47 0.4570447133610642 1103 48-49 0.41377175073958483 1103 50-51 0.4484121525730487 1103 52-53 0.246658867993105 1103 54-55 0.26110717593304855 1103 56-57 0.46576900553250056 1103 58-59 0.49295337564269914 1103 60-61 0.45292064229415274 1103 62-63 0.385803607653159 1103 64-65 0.4731119863285542 1103 66-67 0.35178422616494487 1103 68-69 0.20028613080541646 1103 70-71 0.43929061454416285 1103 72-73 0.45238675707102516 1103 74-75 0.2988965261096048 1103 76-77 0.44338767729774986 1103 78-79 0.37106873677842245 1103 80-81 0.5650175895776286 1103 82-83 0.5791292249636086 1103 84-85 0.4374128668397361 1103 86-87 0.5903311978712935 1103 88-89 0.6998127411812014 1103 90-91 0.858003255264304 1103 92-93 0.7084789916891232 1103 94-95 0.6278468609664003 1103 96-97 0.37285488585811066 1103 98-99 0.15120853836079462 1103 100-101 0.2592348979819086 1104 1 0.08035145639628638 1104 2 0.08697977798048129 1104 3 0.06801245455795168 1104 4 0.05382454434079165 1104 5 0.12440430885568787 1104 6 0.013356912495950723 1104 7 0.07823147418655196 1104 8 0.15949408979123803 1104 9 0.19214441772642488 1104 10-11 0.05682395676368657 1104 12-13 0.1817696835225746 1104 14-15 0.13826541362722367 1104 16-17 0.0928132538705313 1104 18-19 0.03899802776562211 1104 20-21 0.09490184329755635 1104 22-23 0.10037937026212518 1104 24-25 0.14057297450170125 1104 26-27 0.16169839034346012 1104 28-29 0.11574877575458231 1104 30-31 0.11468379086931435 1104 32-33 0.1051288595246973 1104 34-35 0.24920544780210463 1104 36-37 0.2028678446906227 1104 38-39 0.1807460031034296 1104 40-41 0.2816347475567227 1104 42-43 0.3077458478863875 1104 44-45 0.2848121846503844 1104 46-47 0.2597885005357483 1104 48-49 0.29911701966927495 1104 50-51 0.23131129605565093 1104 52-53 0.32845471108788615 1104 54-55 0.40314804264262705 1104 56-57 0.2032124290745756 1104 58-59 0.19767544351788047 1104 60-61 0.32712256438743736 1104 62-63 0.39374225203560087 1104 64-65 0.22136167922983674 1104 66-67 0.18178466189925047 1104 68-69 0.16759090745165395 1104 70-71 0.12106763062863024 1104 72-73 0.09643100324657183 1104 74-75 0.01387790522158383 1104 76-77 0.15210340858616433 1104 78-79 0.37048460617463874 1104 80-81 0.31419958945242854 1104 82-83 0.36253756684426364 1104 84-85 0.40279236733789503 1104 86-87 0.3025229966001035 1104 88-89 0.4341410755498494 1104 90-91 0.4691758115671085 1104 92-93 0.24564483721391284 1104 94-95 0.20136880265481594 1104 96-97 0.18566401790944198 1104 98-99 0.3485617050040304 1104 100-101 0.2992622024320504 1105 1 0.09751701029076543 1105 2 0.11370998653225683 1105 3 0.07926114955915864 1105 4 0.09619151338385024 1105 5 0.09796621709651276 1105 6 0.12860606297837762 1105 7 0.07999229242924599 1105 8 0.10218496911802788 1105 9 0.18821115699950042 1105 10-11 0.1639073098270245 1105 12-13 0.19082147083539525 1105 14-15 0.2223292644278132 1105 16-17 0.19829875851892353 1105 18-19 0.14552048501916914 1105 20-21 0.15463048246849098 1105 22-23 0.15142382026855472 1105 24-25 0.150458100784995 1105 26-27 0.2069105949853025 1105 28-29 0.19704095568319246 1105 30-31 0.22479891262033647 1105 32-33 0.21078528237362093 1105 34-35 0.35648300046992887 1105 36-37 0.17149640651994247 1105 38-39 0.38853405551533626 1105 40-41 0.37843944532690443 1105 42-43 0.34092246862160636 1105 44-45 0.3770758812187154 1105 46-47 0.41515449773521595 1105 48-49 0.47252345548363195 1105 50-51 0.5160215017663035 1105 52-53 0.4120724383267955 1105 54-55 0.531145142895852 1105 56-57 0.5303597298973699 1105 58-59 0.48413073983998345 1105 60-61 0.44879687502112375 1105 62-63 0.49076798221484097 1105 64-65 0.4579083536532096 1105 66-67 0.38700612273124335 1105 68-69 0.4706928022084256 1105 70-71 0.45338847656097414 1105 72-73 0.4476029654461442 1105 74-75 0.46490330168190397 1105 76-77 0.591926109138825 1105 78-79 0.5911795194415426 1105 80-81 0.6949718556067914 1105 82-83 0.7047680042640749 1105 84-85 0.6391745499046912 1105 86-87 0.613240616282301 1105 88-89 0.8983697338870691 1105 90-91 0.8589850393460523 1105 92-93 0.7475552088820798 1105 94-95 0.7592123809333238 1105 96-97 0.6303807739812122 1105 98-99 0.6894385514504862 1105 100-101 0.7791615568663381 1106 1 -0.030419722172425168 1106 2 -0.0028827394155825914 1106 3 -0.0029019915283114983 1106 4 0.002177496795411571 1106 5 -0.07366948019661379 1106 6 0.060173391896697126 1106 7 -0.05176578211156624 1106 8 -0.05634500366321049 1106 9 -0.07901167246533447 1106 10-11 0.029738660691812413 1106 12-13 0.05194151198501373 1106 14-15 0.0561507136659003 1106 16-17 0.07215053416778261 1106 18-19 0.09477947042390866 1106 20-21 0.09234292808455535 1106 22-23 0.10738837158769599 1106 24-25 0.11877128795145353 1106 26-27 0.13238210101844317 1106 28-29 0.11162495876941136 1106 30-31 0.15647929582615205 1106 32-33 0.053963447357865846 1106 34-35 0.10200369315516156 1106 36-37 0.05396549072663248 1106 38-39 0.09034185251055504 1106 40-41 0.09322705329105929 1106 42-43 0.15010397701227163 1106 44-45 0.24928703019318732 1106 46-47 0.28023304635391355 1106 48-49 0.2823672155299235 1106 50-51 0.3022111972162733 1106 52-53 0.45976795258985703 1106 54-55 0.32824002276896636 1106 56-57 0.347019431134008 1106 58-59 0.27203125206699497 1106 60-61 0.33595530104825855 1106 62-63 0.30617337024287394 1106 64-65 0.3508320125400246 1106 66-67 0.3952253301724653 1106 68-69 0.4545533650151796 1106 70-71 0.3607980867112133 1106 72-73 0.33971488383273396 1106 74-75 0.38295340991397 1106 76-77 0.4733241245270676 1106 78-79 0.5280421825933495 1106 80-81 0.47493820722169744 1106 82-83 0.592433835079138 1106 84-85 0.5720197673332699 1106 86-87 0.5509562767122276 1106 88-89 0.734406189876271 1106 90-91 0.8394598673535079 1106 92-93 0.7129868512233237 1106 94-95 0.8776670199097261 1106 96-97 0.9147310600996441 1106 98-99 0.8791093545109874 1106 100-101 0.6353377500980848 1107 1 0.03400873108444813 1107 2 -0.014256039660835995 1107 3 0.06349205095960286 1107 4 0.008091679264950358 1107 5 0.10949665055104418 1107 6 0.0796053799385561 1107 7 0.027043130107593072 1107 8 -0.028644521232443765 1107 9 0.11330820380682383 1107 10-11 0.13565035533614633 1107 12-13 0.027239584542442685 1107 14-15 0.10165956721385783 1107 16-17 0.09051804399987162 1107 18-19 0.07565431415738288 1107 20-21 0.1430363057011732 1107 22-23 0.1302510705878035 1107 24-25 0.16948871454506076 1107 26-27 0.10019378923589528 1107 28-29 0.10571481901583013 1107 30-31 0.04581119036723891 1107 32-33 0.057888302597540076 1107 34-35 0.15164275118160475 1107 36-37 0.17895776810255626 1107 38-39 0.0896037976996169 1107 40-41 0.15465321982193814 1107 42-43 0.1069507829556926 1107 44-45 0.11631588274600801 1107 46-47 0.043737168887602706 1107 48-49 0.0855304852785892 1107 50-51 0.1585092303831246 1107 52-53 0.1785072886307688 1107 54-55 0.18845897211892293 1107 56-57 0.24674027641408003 1107 58-59 0.1769946706705383 1107 60-61 0.16594976563717978 1107 62-63 0.04678276302693973 1107 64-65 0.14611499229120284 1107 66-67 0.19575751744765313 1107 68-69 0.2761250900524388 1107 70-71 0.22499258073939643 1107 72-73 0.3569597625408889 1107 74-75 0.24692047525462968 1107 76-77 0.06443927144351491 1107 78-79 0.14928043539370606 1107 80-81 0.18305090155713089 1107 82-83 0.4022948485776645 1107 84-85 0.39552650208342044 1107 86-87 0.49816887588834646 1107 88-89 0.7644902161725788 1107 90-91 0.7940772709347321 1107 92-93 0.7581882510396376 1107 94-95 0.9587984206175122 1107 96-97 0.909480815981798 1107 98-99 1.0119856536222152 1107 100-101 0.837094258352483 1108 1 0.058137373422965766 1108 2 0.049506553274703435 1108 3 0.057658744972485465 1108 4 0.08696508147271942 1108 5 0.04556141972276606 1108 6 0.005819801908366173 1108 7 0.035115735042182905 1108 8 0.014432199903147591 1108 9 0.05713811125293944 1108 10-11 0.04664910121459087 1108 12-13 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-0.4726739991615645 2316 82-83 -0.47567745779049986 2316 84-85 -0.443363996909401 2316 86-87 -0.6202099756866275 2316 88-89 -1.031150097434388 2316 90-91 -1.1686980217926752 2316 92-93 -0.8151328155212418 2316 94-95 -0.5320669458016454 2316 96-97 -0.3816520632671825 2316 98-99 -0.18446482067118097 2316 100-101 0.5174770538449067 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 5.0 4 27.0 5 59.0 6 127.0 7 318.0 8 694.0 9 1408.0 10 2289.0 11 3504.0 12 5016.0 13 6726.0 14 8898.0 15 11431.0 16 14949.0 17 19833.0 18 19539.0 19 15493.0 20 15876.0 21 17535.0 22 18881.0 23 21306.0 24 24926.0 25 29501.0 26 35474.0 27 43251.0 28 52558.0 29 64703.0 30 78067.0 31 96151.0 32 119067.0 33 153120.0 34 209704.0 35 300017.0 36 473776.0 37 723068.0 38 491026.0 39 27133.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.29730912463792 19.132669950745278 15.554589544538363 23.015431380078436 2 15.552114306985562 20.016892816592186 39.596588204911214 24.834404671511034 3 19.68003472591196 23.61532680161599 29.176367316682672 27.52827115578938 4 13.583647135273269 16.607062008808995 36.14821240768684 33.66107844823089 5 14.543278906648997 37.042926302108995 33.47067199663045 14.943122794611558 6 33.1193555097438 35.49703715582416 16.901993970623373 14.481613363808671 7 29.918511319732783 31.804124474876843 19.744469582947723 18.53289462244265 8 26.933609234310385 35.27375314848483 18.840513907431486 18.952123709773296 9 26.327515841443237 17.402209397506716 19.711753033848105 36.55852172720194 10-11 25.673474671401546 26.376155947166634 26.448721639485527 21.501647741946286 12-13 26.510676994276537 23.127702093923546 27.164621560334663 23.19699935146526 14-15 23.15635260811483 24.803685563935364 25.31761068212011 26.72235114582969 16-17 23.03498670235461 28.17170337057232 25.826413480614807 22.96689644645826 18-19 23.097250794205884 27.051285952598658 27.60603459615732 22.245428657038143 20-21 23.825520969182683 26.036705649594165 27.50387864994001 22.63389473128314 22-23 23.455495510812884 25.771527714716846 27.503814247284303 23.269162527185973 24-25 23.42818878479273 26.09503835500161 27.500755121138177 22.97601773906749 26-27 23.223894938625858 26.225043560360284 27.584616261482598 22.966445239531264 28-29 22.99396997934607 26.595591895827415 27.521492776276123 22.888945348550394 30-31 23.22490180205071 26.136980584531383 27.376699505580817 23.261418107837095 32-33 23.03625032281831 26.190885607358904 27.404167238240234 23.368696831582547 34-35 23.60458039357122 26.187942976933805 27.223957545058465 22.983519084436516 36-37 23.618333032496352 25.93581367923199 27.39968482160152 23.04616846667013 38-39 23.2931416524091 26.220474925846172 27.397839032978045 23.08854438876668 40-41 23.476493799514163 26.09311347487241 27.316578118199764 23.113814607413662 42-43 23.310124680762957 26.137781597584375 27.038713539514873 23.513380182137798 44-45 23.37545637860957 26.190786706880154 27.020141520036223 23.41361539447406 46-47 23.599145825239372 26.422243965333887 26.75222797572078 23.226382233705962 48-49 23.565372280364656 26.334811219699827 26.738247309527107 23.361569190408407 50-51 23.35449604599895 26.49079267432672 26.80659109659046 23.34812018308387 52-53 23.688499660517337 26.581291244889442 26.452035052450352 23.27817404214286 54-55 23.390110714605427 26.309064480582922 26.64160759333072 23.659217211480932 56-57 23.486248399380635 26.431355829458546 26.702509162554662 23.37988660860616 58-59 23.72324016229793 26.186771430411543 26.6383235654022 23.451664841888324 60-61 23.407075350107 26.310010148308926 27.016978412262212 23.265936089321865 62-63 23.339512833814588 26.362219197412017 26.937952856111025 23.360315112662374 64-65 23.186670191639823 26.42652733362293 26.845869260634736 23.54093321410251 66-67 23.192587089716877 26.45008914980678 26.600872595797 23.75645116467935 68-69 23.15786355193636 26.28900604621088 26.845710848401634 23.707419553451132 70-71 23.355767998449185 26.232924440872246 26.692984811921704 23.71832274875687 72-73 23.3683104156483 26.111702542166025 26.74692203607708 23.77306500610859 74-75 23.125648655706836 26.628478026289816 26.482718668822443 23.763154649180912 76-77 23.340253534072424 26.22204013716429 26.614911248869433 23.822795079893854 78-79 23.24185353657979 26.386687567502122 26.566081396045288 23.805377499872804 80-81 23.387979584979156 26.468023964899224 26.518483502516617 23.625512947605 82-83 23.279032234173208 26.38734590859589 26.652024722891472 23.681597134339434 84-85 23.253206769234335 26.3212848845035 26.570877376699503 23.85463096956266 86-87 23.30093973352726 26.276354251688183 26.63574945940823 23.786956555376328 88-89 23.181582819198727 26.300506291145854 26.464120041165245 24.053790848490177 90-91 23.2099701043466 26.193400353284378 26.572290667057157 24.02433887531186 92-93 23.292427986560455 26.1434369507661 26.67007356715361 23.894061495519832 94-95 23.063041827592805 26.055978144314757 26.693500033167368 24.18747999492507 96-97 22.955843607166983 25.984265787184 26.815188851127463 24.24470175452155 98-99 22.975171802189756 26.135073021364626 26.435366600965203 24.454388575480422 100-101 23.182387855150047 25.506545271406893 26.985526725934548 24.325540147508505 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 91.0 1 67.0 2 67.5 3 94.5 4 87.0 5 89.5 6 164.0 7 497.5 8 877.5 9 1009.5 10 990.0 11 995.5 12 1127.0 13 1278.0 14 1485.0 15 1644.0 16 1749.5 17 1943.0 18 2132.0 19 2320.0 20 2608.5 21 3027.0 22 3620.5 23 4494.0 24 5569.0 25 7043.5 26 9366.5 27 12785.5 28 16105.5 29 20021.0 30 25473.5 31 30344.5 32 35649.0 33 41188.5 34 46826.0 35 53002.0 36 60296.0 37 68385.5 38 73861.5 39 77761.0 40 82901.0 41 89295.5 42 95118.5 43 100555.5 44 107102.0 45 113865.5 46 118082.0 47 125303.0 48 140574.0 49 142448.5 50 131887.5 51 125932.0 52 122290.5 53 120635.5 54 119293.5 55 113151.5 56 103541.0 57 94930.0 58 86100.0 59 75565.0 60 63300.0 61 52001.5 62 41898.5 63 31826.5 64 23703.5 65 18070.5 66 13376.0 67 10355.0 68 7608.5 69 5037.5 70 3645.0 71 2665.5 72 1932.0 73 1528.5 74 1250.5 75 979.5 76 659.5 77 384.0 78 234.5 79 125.0 80 79.5 81 52.0 82 35.5 83 21.0 84 10.0 85 7.5 86 5.5 87 3.0 88 1.0 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2933218954216796 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.001561764400916836 16-17 8.050331963488847E-4 18-19 4.830199178093308E-5 20-21 0.0 22-23 0.0 24-25 0.0 26-27 3.220132785395538E-5 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0016744690484056801 36-37 0.0076156140374604485 38-39 0.0029947234904178508 40-41 0.0032362334493225165 42-43 3.5421460639350924E-4 44-45 0.001159247802742394 46-47 2.7371128675862075E-4 48-49 4.347179260283977E-4 50-51 0.0 52-53 4.830199178093308E-5 54-55 0.0 56-57 7.567312045679516E-4 58-59 0.001996482326945234 60-61 5.957245652981746E-4 62-63 0.0010626438191805277 64-65 3.220132785395538E-5 66-67 4.8301991780933077E-4 68-69 0.0014812610812819477 70-71 0.0 72-73 0.0 74-75 3.220132785395538E-5 76-77 0.2578843341184017 78-79 1.2880531141582153E-4 80-81 1.1270464748884384E-4 82-83 0.0 84-85 0.0 86-87 0.1064575898851765 88-89 0.3732133898273429 90-91 0.2744680179631887 92-93 0.0 94-95 0.0 96-97 0.0 98-99 3.220132785395538E-5 100-101 4.5081858995537537E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3105462.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.16500938277958 #Duplication Level Percentage of deduplicated Percentage of total 1 70.08125416611625 33.75464264467949 2 15.28096526803566 14.72015671025733 3 5.684374013465446 8.213637830813749 4 2.8329317570181285 5.457927386302096 5 1.6498294494550598 3.9732025456494515 6 1.0191607936159937 2.945273351224525 7 0.6826899735954433 2.3017238288627846 8 0.4820521907488306 1.85744386323255 9 0.33864714758621384 1.4679848736847374 >10 1.7851219750233622 15.33310529575096 >50 0.11209536134349646 3.6961084583584256 >100 0.046588317495295455 3.940733442738278 >500 0.0025469419855042447 0.7891778960247586 >1k 0.0016085949371675771 1.1945169145524595 >5k 1.340495780972981E-4 0.3543649578682868 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5833 0.18783034537212176 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5144 0.1656436304807465 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4071 0.13109160569345238 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 3.220132785395539E-5 0.0 0.0 0.0 7 0.0 3.220132785395539E-5 0.0 0.0 0.0 8 0.0 3.220132785395539E-5 0.0 0.0 0.0 9 0.0 3.220132785395539E-5 0.0 3.220132785395539E-5 0.0 10-11 0.0 3.220132785395539E-5 0.0 3.220132785395539E-5 0.0 12-13 0.0 3.220132785395539E-5 0.0 3.220132785395539E-5 0.0 14-15 0.0 4.830199178093308E-5 0.0 6.440265570791078E-5 1.6100663926977694E-5 16-17 0.0 6.440265570791078E-5 0.0 9.660398356186616E-5 3.220132785395539E-5 18-19 0.0 6.440265570791078E-5 0.0 1.6100663926977692E-4 3.220132785395539E-5 20-21 0.0 6.440265570791078E-5 0.0 2.415099589046654E-4 6.440265570791078E-5 22-23 0.0 6.440265570791078E-5 0.0 3.0591261461257615E-4 1.2880531141582153E-4 24-25 0.0 6.440265570791078E-5 0.0 3.8641593424746463E-4 1.6100663926977692E-4 26-27 0.0 6.440265570791078E-5 0.0 6.118252292251524E-4 1.6100663926977692E-4 28-29 0.0 6.440265570791078E-5 0.0 0.0017549723680405685 1.6100663926977692E-4 30-31 0.0 8.050331963488846E-5 0.0 0.0045242865634807315 1.6100663926977692E-4 32-33 0.0 9.660398356186616E-5 0.0 0.01025612292148479 1.6100663926977692E-4 34-35 0.0 9.660398356186616E-5 0.0 0.01911148808132252 1.9320796712373232E-4 36-37 0.0 9.660398356186616E-5 0.0 0.034342716156243416 1.9320796712373232E-4 38-39 0.0 9.660398356186616E-5 0.0 0.060506295037582175 2.0930863105071001E-4 40-41 0.0 9.660398356186616E-5 0.0 0.09164497907235702 2.254092949776877E-4 42-43 0.0 9.660398356186616E-5 0.0 0.12524706468795946 2.254092949776877E-4 44-45 0.0 9.660398356186616E-5 0.0 0.16498350325974043 2.415099589046654E-4 46-47 0.0 9.660398356186616E-5 0.0 0.21446084350734285 2.576106228316431E-4 48-49 0.0 1.2880531141582155E-4 0.0 0.2723105289969737 2.576106228316431E-4 50-51 0.0 1.2880531141582155E-4 0.0 0.3378241305158459 2.576106228316431E-4 52-53 0.0 1.2880531141582155E-4 0.0 0.40295131610047075 2.737112867586208E-4 54-55 0.0 1.2880531141582155E-4 0.0 0.47654745091068573 2.8981195068559845E-4 56-57 0.0 1.2880531141582155E-4 0.0 0.5663891556232212 2.8981195068559845E-4 58-59 0.0 1.2880531141582155E-4 0.0 0.6679682443385235 2.8981195068559845E-4 60-61 0.0 1.2880531141582155E-4 0.0 0.7840540312520328 2.8981195068559845E-4 62-63 0.0 1.2880531141582155E-4 0.0 0.9041166821555053 2.8981195068559845E-4 64-65 0.0 1.2880531141582155E-4 0.0 1.0310382158918705 2.8981195068559845E-4 66-67 0.0 1.2880531141582155E-4 0.0 1.1775059556355867 2.8981195068559845E-4 68-69 0.0 1.2880531141582155E-4 0.0 1.3524235685382724 3.0591261461257615E-4 70-71 0.0 1.2880531141582155E-4 0.0 1.5391107667715787 3.5421460639350924E-4 72-73 0.0 1.2880531141582155E-4 0.0 1.7372938390487471 6.118252292251523E-4 74-75 0.0 1.2880531141582155E-4 0.0 1.9427383107569824 6.118252292251523E-4 76-77 0.0 1.2880531141582155E-4 0.0 2.1676001831611527 6.2792589315213E-4 78-79 0.0 1.2880531141582155E-4 0.0 2.433293339284139 6.440265570791077E-4 80-81 0.0 1.6100663926977692E-4 0.0 2.73134882990035 6.923285488600408E-4 82-83 0.0 2.0930863105071001E-4 0.0 3.0383401889960333 8.694358520567955E-4 84-85 0.0 2.576106228316431E-4 0.0 3.357712958651563 8.694358520567955E-4 86-87 0.0 2.576106228316431E-4 0.0 3.6944100426925206 8.694358520567955E-4 88-89 0.0 2.576106228316431E-4 0.0 4.06487021898835 8.694358520567955E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 12435 0.0 23.001528 1 TACATGG 12390 0.0 22.366346 2 TATACCG 150 3.0591982E-5 22.144217 5 ACATGGG 12120 0.0 21.572601 3 GTATCAA 17575 0.0 21.20286 1 AGTACTT 7485 0.0 20.160572 12-13 GAGTACT 6995 0.0 19.469809 12-13 CATGGGG 7380 0.0 19.032196 4 AGAGTAC 15185 0.0 18.718866 10-11 GTACTTT 7280 0.0 18.349089 14-15 ATCAACG 20105 0.0 18.338781 3 TATCAAC 20340 0.0 18.220219 2 CAACGCA 20320 0.0 18.191448 5 TCAACGC 20270 0.0 18.1895 4 AACGCAG 20645 0.0 17.859104 6 GTAAGGT 1115 0.0 17.0231 4 TACTTTT 8615 0.0 17.020437 14-15 GGTATCA 13725 0.0 16.956045 1 TAAGGTG 1245 0.0 16.389008 5 ACGCAGA 22570 0.0 16.272825 7 >>END_MODULE