FastQCFastQC Report
Fri 27 May 2016
SRR939294_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939294_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3154790
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT54350.1722777110362338No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52760.16723775592036239No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35580.11278088240421708No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG130000.024.5185261
TACATGG129250.023.9569822
ACATGGG129450.023.0774573
GTATCAA166650.021.8342691
GAGTACT63650.020.13954412-13
CATGGGG80650.019.2261714
TATCAAC190750.018.992332
ATCAACG190800.018.9625023
TCAACGC190900.018.952574
CAACGCA191750.018.8682545
GTATAGG6850.018.7235581
AACGCAG196400.018.482526
GTACTTT70600.018.19081914-15
AGTACTT70050.018.16422712-13
GGTATCA128850.018.1382711
AGAGTAC147800.017.60253510-11
ACGCAGA215300.016.9700937
ATACGTC5350.016.8400145
CGCAGAG215750.016.8248758
CATGGGA58450.016.6310184
Produced by FastQC (version 0.11.5)