##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939294_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3154790 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.439801381391472 33.0 31.0 34.0 28.0 34.0 2 31.538363250802746 33.0 31.0 34.0 28.0 34.0 3 31.590843130604572 33.0 31.0 34.0 28.0 34.0 4 35.08276588933019 37.0 35.0 37.0 32.0 37.0 5 35.11097822675994 37.0 35.0 37.0 32.0 37.0 6 35.18782201033983 37.0 35.0 37.0 32.0 37.0 7 35.08660576456753 37.0 35.0 37.0 32.0 37.0 8 35.05611086633342 37.0 35.0 37.0 32.0 37.0 9 36.61414642496014 39.0 37.0 39.0 32.0 39.0 10-11 36.498538729994706 39.0 37.0 39.0 32.0 39.0 12-13 36.37098142824087 39.0 37.0 39.0 32.0 39.0 14-15 37.53082851790452 40.0 37.0 41.0 32.0 41.0 16-17 37.44859229932896 40.0 37.0 41.0 32.0 41.0 18-19 37.35081510972204 40.0 37.0 41.0 31.5 41.0 20-21 37.2141346967627 40.0 37.0 41.0 31.0 41.0 22-23 37.13215982680305 40.0 37.0 41.0 31.0 41.0 24-25 37.14815502775145 40.0 37.0 41.0 31.0 41.0 26-27 37.05533791472649 40.0 37.0 41.0 30.5 41.0 28-29 36.906112134246655 40.0 37.0 41.0 30.0 41.0 30-31 36.73388403031581 40.0 36.0 41.0 30.0 41.0 32-33 36.54367802611267 39.5 36.0 41.0 30.0 41.0 34-35 36.22980515343335 39.0 35.5 41.0 29.5 41.0 36-37 35.97590124857756 39.0 35.0 41.0 27.5 41.0 38-39 35.63250073697456 39.0 35.0 40.5 26.5 41.0 40-41 35.50311557980088 39.0 35.0 40.0 26.0 41.0 42-43 35.343339651767636 39.0 35.0 40.0 25.5 41.0 44-45 35.34162051990782 39.0 35.0 41.0 25.5 41.0 46-47 35.182425137647826 39.0 35.0 41.0 24.5 41.0 48-49 34.998517650937146 39.0 34.5 41.0 24.0 41.0 50-51 33.8790138170845 38.0 33.0 40.0 23.0 40.5 52-53 34.00286199715353 38.0 33.5 39.5 23.0 40.5 54-55 34.486170711838184 38.0 34.0 40.0 23.0 41.0 56-57 34.353841618618034 38.0 34.0 40.0 23.0 41.0 58-59 34.11640885764187 38.0 34.0 40.0 22.0 41.0 60-61 33.79120828961674 37.5 33.0 40.0 21.5 41.0 62-63 33.402337873519315 37.0 33.0 40.0 20.0 41.0 64-65 32.998480247496666 36.5 32.5 39.5 18.5 41.0 66-67 32.614104742312485 36.0 32.0 39.0 17.5 41.0 68-69 32.17177530041619 35.5 31.5 39.0 13.5 40.5 70-71 31.734035070480125 35.0 31.0 38.0 10.5 40.0 72-73 31.276922711178877 35.0 31.0 37.0 7.5 39.5 74-75 30.807766919509696 35.0 30.5 37.0 7.0 39.0 76-77 30.344094060143465 35.0 30.0 36.0 2.0 39.0 78-79 29.899598229993124 34.0 30.0 36.0 2.0 38.0 80-81 29.424564709536927 34.0 29.0 35.0 2.0 37.0 82-83 28.96766852944253 34.0 29.0 35.0 2.0 37.0 84-85 28.536508452226613 34.0 29.0 35.0 2.0 36.0 86-87 28.17663727221146 34.0 27.5 35.0 2.0 36.0 88-89 27.73340507609064 34.0 26.5 35.0 2.0 35.5 90-91 27.306506455263268 33.5 26.0 35.0 2.0 35.0 92-93 26.84929519872955 33.0 25.0 35.0 2.0 35.0 94-95 26.328221371311564 33.0 24.5 35.0 2.0 35.0 96-97 25.697594927079138 33.0 22.0 35.0 2.0 35.0 98-99 24.882486314461502 32.0 10.5 35.0 2.0 35.0 100-101 22.385645478779885 29.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5293749455615888 1101 2 0.31760519584678804 1101 3 0.5247614544415349 1101 4 0.5706418660170556 1101 5 0.552866357363726 1101 6 0.5576134731402718 1101 7 0.5627357720938875 1101 8 0.5024120552896392 1101 9 0.6661741795116498 1101 10-11 0.6126385697467853 1101 12-13 0.6573856461878194 1101 14-15 0.7623877585101653 1101 16-17 0.7910950300634241 1101 18-19 0.7578270659204449 1101 20-21 0.7506942730041786 1101 22-23 0.8515627969486985 1101 24-25 0.7388967028620996 1101 26-27 0.7689215114304915 1101 28-29 0.8489989808587382 1101 30-31 0.8991382362437719 1101 32-33 0.9280764267023827 1101 34-35 0.9586322723921157 1101 36-37 0.8676391422142444 1101 38-39 1.0734264698305225 1101 40-41 0.9067373314081166 1101 42-43 0.9002869336188155 1101 44-45 1.0294931642042897 1101 46-47 1.1333293302709038 1101 48-49 0.9805398169557975 1101 50-51 0.9671372762863086 1101 52-53 0.9959136852146884 1101 54-55 1.003754872737403 1101 56-57 0.9096910477377307 1101 58-59 0.9364136740769098 1101 60-61 0.97603421800072 1101 62-63 1.1148662591545957 1101 64-65 1.1069852063098153 1101 66-67 1.0727711983488675 1101 68-69 1.041003866590927 1101 70-71 1.075231534322537 1101 72-73 1.0256042181975538 1101 74-75 1.1436680486688822 1101 76-77 0.9158104117119805 1101 78-79 0.9898242336277718 1101 80-81 0.8572738720868749 1101 82-83 0.7506647075988617 1101 84-85 0.7099646016422518 1101 86-87 0.7494783308883726 1101 88-89 0.6844829588825334 1101 90-91 0.8292744088272421 1101 92-93 0.8338623333361923 1101 94-95 1.0350089279959676 1101 96-97 0.9219430366994743 1101 98-99 0.8606380283487418 1101 100-101 0.840216250560406 1102 1 0.5119026683992374 1102 2 0.5087403944972273 1102 3 0.4963136702112827 1102 4 0.5464914924082223 1102 5 0.4659869934508265 1102 6 0.5100032252829365 1102 7 0.5010419925924765 1102 8 0.5462297596310464 1102 9 0.5467982866348606 1102 10-11 0.6491221853081299 1102 12-13 0.6683847161128114 1102 14-15 0.8512781584519971 1102 16-17 0.8579538596042084 1102 18-19 0.8535679778341105 1102 20-21 0.8141241686003937 1102 22-23 0.7936146146616494 1102 24-25 0.8780877026640255 1102 26-27 0.8765031178575811 1102 28-29 0.9115698488426318 1102 30-31 0.9277216564128139 1102 32-33 0.8833496984325961 1102 34-35 0.8095872737939231 1102 36-37 0.8440841792785037 1102 38-39 1.0607468419179327 1102 40-41 0.9518228849127937 1102 42-43 0.9102181994489911 1102 44-45 0.9927663249379037 1102 46-47 1.0577198297269277 1102 48-49 1.0308890376352338 1102 50-51 1.0134814093023436 1102 52-53 1.094183477775431 1102 54-55 1.029724071657668 1102 56-57 1.0006189171674507 1102 58-59 0.9931837135094099 1102 60-61 0.9002657578776834 1102 62-63 0.9845402161931602 1102 64-65 1.087370981712013 1102 66-67 1.059166160155506 1102 68-69 0.9123045578318596 1102 70-71 0.9606608495787263 1102 72-73 0.965238929324105 1102 74-75 0.7891017104947586 1102 76-77 0.9103090826233071 1102 78-79 0.8395180839373673 1102 80-81 0.8668029546329485 1102 82-83 0.5193991525249295 1102 84-85 0.615191740408104 1102 86-87 0.5515710235799176 1102 88-89 0.5095229439745417 1102 90-91 0.5725437444838484 1102 92-93 0.7666833120774719 1102 94-95 0.664133982896228 1102 96-97 0.5046194806361441 1102 98-99 0.5208552898519621 1102 100-101 0.35670941326516115 1103 1 0.4222687527605693 1103 2 0.5338227238315021 1103 3 0.6187362608723781 1103 4 0.5737526432212547 1103 5 0.5035969580532154 1103 6 0.4083208503957607 1103 7 0.4619688528966037 1103 8 0.5566223380850843 1103 9 0.601356352933152 1103 10-11 0.6345657872435595 1103 12-13 0.6011872499457169 1103 14-15 0.7080469403166703 1103 16-17 0.7273449237067311 1103 18-19 0.7683773521311679 1103 20-21 0.7961075973218712 1103 22-23 0.7453104836537747 1103 24-25 0.7323317810225447 1103 26-27 0.7933699608064302 1103 28-29 0.7895507872135141 1103 30-31 0.7279294915113255 1103 32-33 0.731888546866621 1103 34-35 0.7200988874300549 1103 36-37 0.8327860722649589 1103 38-39 1.0460477381053295 1103 40-41 0.9131406860254501 1103 42-43 0.7387746549252654 1103 44-45 0.8540722201794111 1103 46-47 0.8516708471847991 1103 48-49 0.8560065551716818 1103 50-51 0.7834885081191914 1103 52-53 0.8127366838429992 1103 54-55 1.0122673233816641 1103 56-57 1.0021900921592888 1103 58-59 1.0199229236372105 1103 60-61 1.0621699584384388 1103 62-63 0.9002177073202304 1103 64-65 0.9444971161815374 1103 66-67 0.8693078094339555 1103 68-69 0.9214459021694168 1103 70-71 0.8243980954730894 1103 72-73 0.8160389521005449 1103 74-75 0.6551786600693923 1103 76-77 0.8111593524112877 1103 78-79 0.8198211612991742 1103 80-81 0.7276227326849174 1103 82-83 0.7760617933130334 1103 84-85 0.6659389279401609 1103 86-87 0.7294893267156297 1103 88-89 0.7573421813211425 1103 90-91 0.6371910365805142 1103 92-93 0.6192300390923187 1103 94-95 0.5991565146397271 1103 96-97 0.5300733390325121 1103 98-99 0.2534020121609295 1103 100-101 0.10253965248375962 1104 1 0.4023036140178533 1104 2 0.5153225873790355 1104 3 0.4446531561628042 1104 4 0.5365752456161772 1104 5 0.5091821145908924 1104 6 0.5548127158350624 1104 7 0.5236029783416782 1104 8 0.5910156928612764 1104 9 0.5724147956060435 1104 10-11 0.6194687135131716 1104 12-13 0.7015097750061159 1104 14-15 0.7261863541828504 1104 16-17 0.7787714649608617 1104 18-19 0.6983536339193819 1104 20-21 0.8646331453257545 1104 22-23 0.7946480189441019 1104 24-25 0.8103413485060216 1104 26-27 0.7944339119887545 1104 28-29 0.7993503888318756 1104 30-31 0.6970878535959173 1104 32-33 0.8386760089125573 1104 34-35 0.8677516872720403 1104 36-37 1.0652236885102013 1104 38-39 1.0246185709145053 1104 40-41 0.8936203640022029 1104 42-43 0.9492481782196833 1104 44-45 0.952230409722091 1104 46-47 0.9556404110662058 1104 48-49 0.9046720248145093 1104 50-51 0.8722826326202124 1104 52-53 1.0092828896738126 1104 54-55 1.0852531829646352 1104 56-57 1.0683981822898616 1104 58-59 1.192156129271197 1104 60-61 1.1867484280031206 1104 62-63 1.039378890109944 1104 64-65 1.1005083458416962 1104 66-67 1.0401564406816348 1104 68-69 1.2159601691859407 1104 70-71 1.0417108548444851 1104 72-73 1.0099237046426772 1104 74-75 1.0418364384215444 1104 76-77 0.9388283386553695 1104 78-79 0.9266871035647739 1104 80-81 0.9602888008396633 1104 82-83 1.036321277561651 1104 84-85 1.0279565524206724 1104 86-87 1.005749953911213 1104 88-89 0.8584515708321057 1104 90-91 1.0034095869213964 1104 92-93 1.1204075860962384 1104 94-95 1.0448057545731189 1104 96-97 0.9673490259521174 1104 98-99 0.8194630521280999 1104 100-101 0.5899788646844222 1105 1 0.31219312234581764 1105 2 0.31602270375581654 1105 3 0.3532576155939644 1105 4 0.40443855193630895 1105 5 0.3876392871386969 1105 6 0.25426900561591026 1105 7 0.33850755733076454 1105 8 0.26570215153426346 1105 9 0.3189566289513053 1105 10-11 0.48391527430081993 1105 12-13 0.513021798398114 1105 14-15 0.46495195172064285 1105 16-17 0.4867117946862507 1105 18-19 0.5297567429556835 1105 20-21 0.47570040527543966 1105 22-23 0.6221794128410778 1105 24-25 0.4759661911057549 1105 26-27 0.5762201498374537 1105 28-29 0.571229688344367 1105 30-31 0.6540062413930556 1105 32-33 0.5646288935457804 1105 34-35 0.6254334829207693 1105 36-37 0.6239979414057046 1105 38-39 0.8551458717418683 1105 40-41 0.7476367548373375 1105 42-43 0.4864324167205183 1105 44-45 0.648428679710932 1105 46-47 0.5480330408899405 1105 48-49 0.5963384926597897 1105 50-51 0.6108209110390845 1105 52-53 0.5881198773442406 1105 54-55 0.6362263473793135 1105 56-57 0.7493163659637148 1105 58-59 0.8454788985615096 1105 60-61 0.8154352392527002 1105 62-63 0.7741707108631815 1105 64-65 0.49487487439377276 1105 66-67 0.7111978405962773 1105 68-69 0.7516940056699255 1105 70-71 0.634731097512617 1105 72-73 0.7252850426310822 1105 74-75 0.7568074237538305 1105 76-77 0.6552411104110689 1105 78-79 0.6021393559459902 1105 80-81 0.6705818503090732 1105 82-83 0.6036233111067943 1105 84-85 0.6367287508285138 1105 86-87 0.6667644599195981 1105 88-89 0.6687560856681571 1105 90-91 0.6092322700557737 1105 92-93 0.667000715359304 1105 94-95 0.45861535791119934 1105 96-97 0.419130200482833 1105 98-99 0.3081352036140892 1105 100-101 0.13040985643200997 1106 1 0.30529910635170054 1106 2 0.3752703878771122 1106 3 0.28401360828120303 1106 4 0.30329312394557917 1106 5 0.3257527268952103 1106 6 0.30303395541726275 1106 7 0.36863440722505914 1106 8 0.3735169016644875 1106 9 0.4463567678529614 1106 10-11 0.450711012266936 1106 12-13 0.4918399342561841 1106 14-15 0.5656550136850811 1106 16-17 0.5708985816662633 1106 18-19 0.5761585772746116 1106 20-21 0.6243698601885725 1106 22-23 0.5948497461029092 1106 24-25 0.5881694930840808 1106 26-27 0.6339600871274484 1106 28-29 0.657521703541839 1106 30-31 0.6408999509864657 1106 32-33 0.6655266476285746 1106 34-35 0.6603197571902584 1106 36-37 0.6132905132311421 1106 38-39 0.7772792377579378 1106 40-41 0.8941077846501244 1106 42-43 0.8270810670253823 1106 44-45 0.9429352979662866 1106 46-47 0.899405716074547 1106 48-49 0.8530135598126094 1106 50-51 0.9801939896512906 1106 52-53 0.7514818846774318 1106 54-55 0.8445424456726585 1106 56-57 0.9347341177995645 1106 58-59 1.0443227465459088 1106 60-61 0.8558616213752401 1106 62-63 1.0003403955768562 1106 64-65 0.9267945514474292 1106 66-67 0.6644513054776553 1106 68-69 0.6420931947293766 1106 70-71 0.782443688539157 1106 72-73 0.6559689034843075 1106 74-75 0.6748714053289326 1106 76-77 0.7881519937643162 1106 78-79 0.784273513497233 1106 80-81 0.8137392841009863 1106 82-83 0.8730012410635908 1106 84-85 0.7780611853864272 1106 86-87 0.9120254622584554 1106 88-89 1.0615669036559225 1106 90-91 0.9956316531690099 1106 92-93 0.8651921851635116 1106 94-95 0.8174763388020096 1106 96-97 0.8270082734266282 1106 98-99 0.8432774501441038 1106 100-101 0.6850736582520298 1107 1 0.038508229095707946 1107 2 0.04701272822410374 1107 3 0.026663234651760348 1107 4 0.02175660403696611 1107 5 0.08007141435343357 1107 6 0.017580312405478082 1107 7 0.039192008015213275 1107 8 -0.033347604037231804 1107 9 0.12909723257016736 1107 10-11 0.10361911139595747 1107 12-13 0.1864147000518841 1107 14-15 0.15907422192987042 1107 16-17 0.17437934648423692 1107 18-19 0.19400773872841626 1107 20-21 0.19382677557572237 1107 22-23 0.20591970310310614 1107 24-25 0.026113458644324794 1107 26-27 0.1084644918644102 1107 28-29 0.1151460637824755 1107 30-31 0.25976798845915283 1107 32-33 0.22391310717782886 1107 34-35 0.2532357628969564 1107 36-37 0.46200301310642544 1107 38-39 0.47837942432324354 1107 40-41 0.31023944787480673 1107 42-43 0.22329788184685384 1107 44-45 0.3872481739652116 1107 46-47 0.3475889491415032 1107 48-49 0.3677024596759324 1107 50-51 0.2591783311356295 1107 52-53 0.2533780915154935 1107 54-55 0.32809763707668793 1107 56-57 0.27060466442592457 1107 58-59 0.3407957145305005 1107 60-61 0.1815845619237635 1107 62-63 0.27621884878089276 1107 64-65 0.3039701074020513 1107 66-67 0.13067251007095138 1107 68-69 0.11379537864086586 1107 70-71 0.018798606580123334 1107 72-73 0.009318723403893614 1107 74-75 0.145929465049786 1107 76-77 -0.04141573841969404 1107 78-79 -0.08632112599275388 1107 80-81 -0.13509430862795213 1107 82-83 -0.060922092569043684 1107 84-85 -0.1486707791362356 1107 86-87 -0.05341132003879068 1107 88-89 -0.11542320938070105 1107 90-91 -0.02147848022019261 1107 92-93 -0.03234904377306336 1107 94-95 -0.05919663816166576 1107 96-97 0.15704803079196594 1107 98-99 0.34930652739410917 1107 100-101 0.23275443703746035 1108 1 0.1757559774545676 1108 2 0.1787112765893184 1108 3 0.12290691422877131 1108 4 0.3345272501409511 1108 5 0.296223769394679 1108 6 0.20537738909144565 1108 7 0.2546706123827107 1108 8 0.2867621882947873 1108 9 0.33857777538404576 1108 10-11 0.3015345176446971 1108 12-13 0.23305596891537306 1108 14-15 0.32438284557182584 1108 16-17 0.40086712852757245 1108 18-19 0.2868090259741507 1108 20-21 0.31547574444691406 1108 22-23 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94-95 -2.348545181380821 2316 96-97 -2.265187887538783 2316 98-99 -2.2374825751884693 2316 100-101 -1.429605650053066 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 17627.0 3 5883.0 4 5927.0 5 5986.0 6 6640.0 7 7675.0 8 9055.0 9 10843.0 10 12749.0 11 14467.0 12 16474.0 13 20052.0 14 18800.0 15 19023.0 16 21740.0 17 25762.0 18 23495.0 19 20902.0 20 21932.0 21 24210.0 22 26139.0 23 29001.0 24 32516.0 25 36867.0 26 42279.0 27 48324.0 28 56455.0 29 65902.0 30 78232.0 31 94396.0 32 116343.0 33 148147.0 34 198059.0 35 277879.0 36 419546.0 37 611901.0 38 498613.0 39 64944.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.124215462302296 18.443267414948885 15.00527183903424 23.42724528371458 2 14.991838206080853 19.3506867711664 40.45469950508811 25.202775517664634 3 19.57572079986188 23.43259670752638 28.81514238272721 28.176540109884527 4 13.074627509886893 15.977966853164297 36.4844651779228 34.46294045902601 5 14.602444299290502 36.91075381235404 33.35894686794398 15.127855020411479 6 33.210187446477846 34.96650702527832 17.217482317866093 14.605823210377746 7 30.028985562411265 31.696622855265044 19.755668647251607 18.518722935072088 8 27.038200750216102 34.7205088146427 19.09295225104483 19.14833818409637 9 26.581211628102462 17.2487855011919 19.714039653802416 36.45596321690322 10-11 25.68257626507919 26.09224255619332 26.543302096496703 21.681879082230783 12-13 26.534134821948136 23.008053320636733 27.15041360955201 23.30739824786312 14-15 23.254823291744067 24.609235300638677 25.304402120533965 26.831539287083295 16-17 23.12505451736864 27.937935048601737 25.830051718149566 23.106958715880058 18-19 23.15724980368814 26.88361446650676 27.56318418707382 22.395951542731275 20-21 23.89465984092009 25.90361942635052 27.440728871981722 22.760991860747666 22-23 23.500671134126257 25.66292538339984 27.427527507616283 23.40887597485761 24-25 23.41291275927957 25.906311545560474 27.50971839844918 23.171057296710774 26-27 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26.4347956109402 26.816420479309766 23.46883977513863 52-53 23.613236247370367 26.49156728081094 26.520899861211085 23.374296610607608 54-55 23.298579417175667 26.214466542258112 26.729834784103762 23.75711925646246 56-57 23.41170213019699 26.360331093656953 26.70777112844866 23.520195647697395 58-59 23.66751014816996 26.11504269449414 26.690338840447176 23.52710831688873 60-61 23.403700787213875 26.183351622461537 27.050963496701204 23.361984093623384 62-63 23.273735260509977 26.280229422761455 27.06072164956651 23.38531366716206 64-65 23.13631209482625 26.35773772102411 26.922165279075156 23.583784905074488 66-67 23.186809620458888 26.342578112695588 26.655868876369066 23.814743390476455 68-69 23.089483981798768 26.205376583773383 26.959385807856684 23.745753626571165 70-71 23.321325946456813 26.138596217673133 26.745117756188524 23.79496007968153 72-73 23.273867796693214 26.00430218644102 26.88007488260618 23.841755134259586 74-75 23.070253697797487 26.527847068615745 26.64563442267015 23.756264810916615 76-77 23.288191770812077 26.089210323012875 26.705596963112395 23.917000943062654 78-79 23.270419748484542 26.23643903395495 26.688241975929817 23.80489924163069 80-81 23.30879353733248 26.341253970588816 26.641758468933002 23.7081940231457 82-83 23.205153958231843 26.241217319969074 26.80713229085504 23.746496430944045 84-85 23.201119248439177 26.17148257791062 26.71294255071833 23.914455622931868 86-87 23.24197328542455 26.127140436014034 26.811831849331625 23.819054429229787 88-89 23.10883392282357 26.12082548252169 26.64696838704495 24.12337220760979 90-91 23.13020754690263 25.9902125524802 26.823435967827468 24.056143932789702 92-93 23.278233079530736 25.917791759892992 26.87976178183774 23.924213378738525 94-95 23.048524977324295 25.7810012821857 26.970861339547564 24.199612400942446 96-97 22.909639344011897 25.712093189617423 27.103709917212303 24.274557549158377 98-99 22.97299146191142 25.856258857383946 26.695192719492894 24.47555696121174 100-101 23.115568537974767 25.24932262469312 27.242074490208974 24.393034347123134 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1981.0 1 1085.0 2 183.5 3 182.5 4 166.0 5 178.0 6 283.0 7 619.0 8 1043.5 9 1256.0 10 1316.5 11 1410.5 12 1591.0 13 1861.0 14 2164.5 15 2325.5 16 2525.5 17 2779.0 18 2896.0 19 2991.0 20 3213.5 21 3592.0 22 4075.0 23 4785.0 24 5830.5 25 7348.0 26 9742.0 27 13201.0 28 16586.5 29 20475.5 30 25554.5 31 30326.5 32 35710.5 33 41366.0 34 47025.0 35 53204.5 36 60705.0 37 68644.5 38 73971.5 39 78030.0 40 83598.5 41 90438.5 42 95846.5 43 101036.0 44 107917.5 45 114590.5 46 118811.5 47 125379.5 48 139982.0 49 142713.0 50 132321.5 51 126175.5 52 122987.5 53 121180.5 54 119909.5 55 114456.5 56 104724.5 57 96367.5 58 88314.5 59 77936.5 60 65278.0 61 53990.5 62 43972.0 63 33505.5 64 25339.5 65 19615.0 66 14809.0 67 11389.5 68 8477.0 69 5903.0 70 4308.5 71 3124.0 72 2338.5 73 1872.0 74 1452.0 75 1110.5 76 791.0 77 495.0 78 317.0 79 211.5 80 137.0 81 104.0 82 83.5 83 54.5 84 32.0 85 27.0 86 27.0 87 20.5 88 16.5 89 13.5 90 7.5 91 8.5 92 9.0 93 5.5 94 5.5 95 6.0 96 2.5 97 2.5 98 4.0 99 1.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2798601491699923 2 0.11163976049118958 3 0.12190985770843701 4 0.12365323840889568 5 0.13034148073247348 6 0.0599089004339433 7 0.06929145838550269 8 0.07496537011972271 9 0.07147860871880536 10-11 0.06662884058843854 12-13 0.0709872923395852 14-15 0.06635940902564037 16-17 0.06735789069953943 18-19 0.07228690340719984 20-21 0.07645516817284193 22-23 0.08441132373311694 24-25 0.09216144339242865 26-27 0.09249427061706168 28-29 0.08704224369926365 30-31 0.08376151819930963 32-33 0.07634422576463093 34-35 0.24272614025022268 36-37 0.36406543700214594 38-39 0.33593361206292655 40-41 0.3194824378167802 42-43 0.34266940113288047 44-45 0.3558398498790728 46-47 0.36164055293696257 48-49 0.3714510316059072 50-51 0.36515901216879726 52-53 0.365396745900678 54-55 0.35233723956269675 56-57 0.3284687728818717 58-59 0.313428152111551 60-61 0.2715711663850843 62-63 0.34273279679471536 64-65 0.3706744347484302 66-67 0.3760313681734759 68-69 0.3884727668085673 70-71 0.38924936366604435 72-73 0.37203744147787965 74-75 0.37523892240054013 76-77 0.3584549209297608 78-79 0.33629813711847695 80-81 0.3352838065291192 82-83 0.3496746217656326 84-85 0.35666399348292593 86-87 0.3498806576665959 88-89 0.3657137242098523 90-91 0.3897406800452645 92-93 0.3843203509583839 94-95 0.3838448834946224 96-97 0.3896931332988884 98-99 0.37821851850677857 100-101 0.3557289074708618 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3154790.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.920206089933885 #Duplication Level Percentage of deduplicated Percentage of total 1 72.75183045261574 37.04538200067121 2 13.917862556132546 14.174008593792866 3 5.357834781233917 8.184661537687408 4 2.5792262487079958 5.25338928547113 5 1.4621349728549333 3.7226107074536543 6 0.8672300601879386 2.6495720035293364 7 0.5812880887173877 2.071951649257921 8 0.4062422267790234 1.6548750328017225 9 0.3167774940026348 1.4517337751340262 >10 1.6212868723218503 14.622175587108204 >50 0.09259289273618528 3.2249226126165196 >100 0.041757786987171384 3.708222872336917 >500 0.002623711149537136 0.9102607366003207 >1k 0.001186916947168424 0.9855220196867815 >5k 1.2493862601772884E-4 0.34071158585195005 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5435 0.1722777110362338 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5276 0.16723775592036239 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3558 0.11278088240421708 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.169783091743032E-5 2 0.0 0.0 0.0 0.0 3.169783091743032E-5 3 0.0 0.0 0.0 0.0 3.169783091743032E-5 4 0.0 0.0 0.0 0.0 3.169783091743032E-5 5 0.0 0.0 0.0 0.0 3.169783091743032E-5 6 0.0 0.0 0.0 0.0 3.169783091743032E-5 7 0.0 0.0 0.0 3.169783091743032E-5 3.169783091743032E-5 8 0.0 0.0 0.0 3.169783091743032E-5 6.339566183486064E-5 9 0.0 0.0 0.0 6.339566183486064E-5 6.339566183486064E-5 10-11 0.0 0.0 0.0 6.339566183486064E-5 7.92445772935758E-5 12-13 0.0 0.0 0.0 9.509349275229096E-5 9.509349275229096E-5 14-15 1.584891545871516E-5 0.0 0.0 1.2679132366972127E-4 1.1094240821100612E-4 16-17 3.169783091743032E-5 0.0 0.0 1.7433807004586674E-4 1.2679132366972127E-4 18-19 3.169783091743032E-5 0.0 0.0 2.2188481642201224E-4 2.2188481642201224E-4 20-21 3.169783091743032E-5 0.0 0.0 3.0112939371558804E-4 2.2188481642201224E-4 22-23 3.169783091743032E-5 0.0 0.0 4.754674637614548E-4 3.0112939371558804E-4 24-25 3.169783091743032E-5 0.0 0.0 4.754674637614548E-4 3.8037397100916385E-4 26-27 3.169783091743032E-5 0.0 0.0 5.230142101376003E-4 3.8037397100916385E-4 28-29 3.169783091743032E-5 0.0 0.0 0.001093575166651346 3.8037397100916385E-4 30-31 3.169783091743032E-5 0.0 0.0 0.0035501570627521958 3.9622288646787903E-4 32-33 3.169783091743032E-5 0.0 0.0 0.009762931922568538 4.4376963284402447E-4 34-35 3.169783091743032E-5 0.0 0.0 0.017877576637430698 4.754674637614548E-4 36-37 3.169783091743032E-5 1.584891545871516E-5 0.0 0.032886499576833955 5.071652946788851E-4 38-39 3.169783091743032E-5 3.169783091743032E-5 0.0 0.05786439033976905 5.071652946788851E-4 40-41 3.169783091743032E-5 3.169783091743032E-5 0.0 0.08669356755917193 5.071652946788851E-4 42-43 3.169783091743032E-5 3.169783091743032E-5 0.0 0.11829630498384996 5.071652946788851E-4 44-45 3.169783091743032E-5 3.169783091743032E-5 0.0 0.1578393490533443 5.230142101376003E-4 46-47 3.169783091743032E-5 3.169783091743032E-5 0.0 0.2062577857797191 5.705609565137457E-4 48-49 3.169783091743032E-5 3.169783091743032E-5 0.0 0.2632663346847175 6.656544492660367E-4 50-51 3.169783091743032E-5 3.169783091743032E-5 0.0 0.32626577363311027 6.656544492660367E-4 52-53 3.169783091743032E-5 3.169783091743032E-5 0.0 0.39267272940512676 7.132011956421823E-4 54-55 3.169783091743032E-5 3.169783091743032E-5 0.0 0.4647218990804459 7.290501111008973E-4 56-57 3.169783091743032E-5 3.169783091743032E-5 0.0 0.5542524225067278 7.290501111008973E-4 58-59 3.169783091743032E-5 9.509349275229096E-5 0.0 0.6541164388120921 7.290501111008973E-4 60-61 3.169783091743032E-5 9.509349275229096E-5 0.0 0.7681176877066302 7.290501111008973E-4 62-63 3.169783091743032E-5 9.509349275229096E-5 0.0 0.8849875902991958 7.290501111008973E-4 64-65 3.169783091743032E-5 9.509349275229096E-5 0.0 1.0064061316284127 7.290501111008973E-4 66-67 3.169783091743032E-5 9.509349275229096E-5 0.0 1.1514395569911149 7.290501111008973E-4 68-69 3.169783091743032E-5 9.509349275229096E-5 0.0 1.3169339322110187 7.607479420183277E-4 70-71 3.169783091743032E-5 9.509349275229096E-5 0.0 1.5015262505586744 7.607479420183277E-4 72-73 3.169783091743032E-5 9.509349275229096E-5 0.0 1.6950415083095864 8.558414347706187E-4 74-75 3.169783091743032E-5 9.509349275229096E-5 0.0 1.9030585237052229 8.558414347706187E-4 76-77 3.169783091743032E-5 9.509349275229096E-5 0.0 2.124721455310813 8.558414347706187E-4 78-79 3.169783091743032E-5 9.509349275229096E-5 0.0 2.3871002507298424 8.558414347706187E-4 80-81 3.169783091743032E-5 9.509349275229096E-5 0.0 2.6801466975614856 8.558414347706187E-4 82-83 3.169783091743032E-5 9.509349275229096E-5 0.0 2.9885190456417066 8.875392656880489E-4 84-85 3.169783091743032E-5 9.509349275229096E-5 0.0 3.305307167830505 8.875392656880489E-4 86-87 3.169783091743032E-5 9.509349275229096E-5 0.0 3.635408379004625 8.875392656880489E-4 88-89 3.169783091743032E-5 9.509349275229096E-5 0.0 4.007556762890715 8.875392656880489E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 13000 0.0 24.518526 1 TACATGG 12925 0.0 23.956982 2 ACATGGG 12945 0.0 23.077457 3 GTATCAA 16665 0.0 21.834269 1 GAGTACT 6365 0.0 20.139544 12-13 CATGGGG 8065 0.0 19.226171 4 TATCAAC 19075 0.0 18.99233 2 ATCAACG 19080 0.0 18.962502 3 TCAACGC 19090 0.0 18.95257 4 CAACGCA 19175 0.0 18.868254 5 GTATAGG 685 0.0 18.723558 1 AACGCAG 19640 0.0 18.48252 6 GTACTTT 7060 0.0 18.190819 14-15 AGTACTT 7005 0.0 18.164227 12-13 GGTATCA 12885 0.0 18.138271 1 AGAGTAC 14780 0.0 17.602535 10-11 ACGCAGA 21530 0.0 16.970093 7 ATACGTC 535 0.0 16.840014 5 CGCAGAG 21575 0.0 16.824875 8 CATGGGA 5845 0.0 16.631018 4 >>END_MODULE