FastQCFastQC Report
Fri 27 May 2016
SRR939294_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939294_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3154790
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT57740.18302327571724267No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53020.16806189952421557No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41050.13011959591605146No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG123500.021.6144221
TACATGG122950.021.2263642
ACATGGG121750.020.539183
GTATCAA184100.020.3666741
GAGTACT71550.019.5988512-13
ACCGTAT1951.0429123E-519.4669028
AGTACTT78050.019.21307812-13
CATGGGG73250.018.980234
GTACTTT77450.018.1671214-15
CGCACCG2403.1244563E-617.7939645
AGAGTAC160600.017.71425810-11
TATCAAC212400.017.5817362
ATCAACG210850.017.5759543
TCAACGC210850.017.5759544
TAAGGTG13500.017.5742875
CAACGCA212300.017.4559125
AACGCAG217500.017.0385746
TACACTG27800.016.8978655
GTGTAGC17100.016.6956331
CCATATA13400.016.6431122
Produced by FastQC (version 0.11.5)