##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939294_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3154790 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.26041099407568 33.0 31.0 34.0 31.0 34.0 2 32.466348631763125 34.0 31.0 34.0 31.0 34.0 3 32.56378522817683 34.0 31.0 34.0 31.0 34.0 4 36.1008637658925 37.0 35.0 37.0 35.0 37.0 5 36.032432586638095 37.0 35.0 37.0 35.0 37.0 6 36.063988728251324 37.0 35.0 37.0 35.0 37.0 7 36.00796344606139 37.0 35.0 37.0 35.0 37.0 8 35.97844801080262 37.0 35.0 37.0 35.0 37.0 9 37.68438913525147 39.0 37.0 39.0 35.0 39.0 10-11 37.59684432244302 39.0 37.0 39.0 35.0 39.0 12-13 37.515945435353856 39.0 37.0 39.0 35.0 39.0 14-15 38.815310527800584 40.0 38.0 41.0 35.0 41.0 16-17 38.75700426969783 40.0 38.0 41.0 34.0 41.0 18-19 38.85850753932908 40.0 38.0 41.0 35.0 41.0 20-21 38.851941492143695 40.0 38.0 41.0 34.5 41.0 22-23 38.797653885044646 40.0 38.0 41.0 34.0 41.0 24-25 38.73438105230459 40.0 38.0 41.0 34.0 41.0 26-27 38.5716409016131 40.0 38.0 41.0 34.0 41.0 28-29 38.42962827953683 40.0 38.0 41.0 34.0 41.0 30-31 38.26685246878556 40.0 38.0 41.0 34.0 41.0 32-33 38.09694686492603 40.0 38.0 41.0 33.0 41.0 34-35 38.08862428244035 40.0 38.0 41.0 33.0 41.0 36-37 38.02916897796684 40.0 38.0 41.0 33.0 41.0 38-39 37.93739504055738 40.0 37.5 41.0 33.0 41.0 40-41 37.78500454863874 40.0 37.0 41.0 33.0 41.0 42-43 37.63404061760053 40.0 37.0 41.0 33.0 41.0 44-45 37.44736511780499 40.0 37.0 41.0 32.0 41.0 46-47 37.21219780080449 40.0 37.0 41.0 31.0 41.0 48-49 36.94608579334916 40.0 36.0 41.0 31.0 41.0 50-51 36.71767185771478 39.0 36.0 41.0 31.0 41.0 52-53 36.48273894617391 39.0 35.0 41.0 30.0 41.0 54-55 36.19686318265241 39.0 35.0 40.0 30.0 41.0 56-57 35.942305985501406 38.5 35.0 40.0 29.0 41.0 58-59 35.625743551868744 38.0 35.0 40.0 28.5 41.0 60-61 35.27097128493497 38.0 34.0 40.0 28.0 41.0 62-63 34.877282640048946 37.0 34.0 40.0 28.0 41.0 64-65 34.43415980144479 37.0 34.0 39.5 26.5 41.0 66-67 34.03640242298219 36.0 33.0 39.0 26.0 40.0 68-69 33.632782213713114 36.0 33.0 39.0 26.0 40.0 70-71 33.21588869623652 35.0 32.5 38.0 26.0 40.0 72-73 32.708668247331836 35.0 32.0 37.0 25.0 39.5 74-75 32.19789700740778 35.0 31.5 37.0 23.5 39.0 76-77 30.225058403253463 33.0 29.5 35.0 20.0 37.0 78-79 31.209856757502084 34.0 31.0 36.0 21.5 37.5 80-81 31.260263123694443 34.0 31.0 35.5 22.0 37.0 82-83 30.9566354654351 34.0 31.0 35.0 20.5 37.0 84-85 30.58615660630343 34.0 31.0 35.0 19.5 36.0 86-87 30.060740810006372 34.0 30.0 35.0 17.0 36.0 88-89 29.56076220604224 34.0 30.0 35.0 9.5 35.5 90-91 29.139098323501724 34.0 29.0 35.0 4.5 35.0 92-93 28.78172826083511 34.0 29.0 35.0 2.0 35.0 94-95 28.374291632723573 34.0 29.0 35.0 2.0 35.0 96-97 27.798147261782876 33.0 28.0 35.0 2.0 35.0 98-99 27.015101639094837 33.0 27.0 35.0 2.0 35.0 100-101 23.59397091407035 29.5 13.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1780873655019164 1101 2 0.1929516102191542 1101 3 0.21307045231359467 1101 4 0.1626683190218543 1101 5 0.22280794110630353 1101 6 0.18021257515042777 1101 7 0.18389125303831122 1101 8 0.2172502330105246 1101 9 0.23643011317484053 1101 10-11 0.25320101699785624 1101 12-13 0.23738258367689724 1101 14-15 0.3523650139619008 1101 16-17 0.3192769155194668 1101 18-19 0.280240412373125 1101 20-21 0.3655306708735395 1101 22-23 0.35813491614110404 1101 24-25 0.3483212753298588 1101 26-27 0.39318586316963433 1101 28-29 0.3704970840097985 1101 30-31 0.3016279236675601 1101 32-33 0.24334005364486444 1101 34-35 0.3228005467076045 1101 36-37 0.34112901276592567 1101 38-39 0.34339628945718204 1101 40-41 0.458728828708729 1101 42-43 0.36422815010411824 1101 44-45 0.47336042968779424 1101 46-47 0.4633132063134511 1101 48-49 0.566623746158534 1101 50-51 0.5524624704187318 1101 52-53 0.48741988826920135 1101 54-55 0.32397799579339903 1101 56-57 0.37255248684007114 1101 58-59 0.290497463668153 1101 60-61 0.419775294565909 1101 62-63 0.42739374649519135 1101 64-65 0.3662737842530177 1101 66-67 0.4105448373725693 1101 68-69 0.44648429926012767 1101 70-71 0.40306199210489524 1101 72-73 0.364450559929935 1101 74-75 0.2856490394241291 1101 76-77 0.42157798138664404 1101 78-79 0.6071146714542799 1101 80-81 0.64015356817821 1101 82-83 0.6382240833966364 1101 84-85 0.5548893023826231 1101 86-87 0.5244106500297789 1101 88-89 0.6188869744729821 1101 90-91 0.7524265997658546 1101 92-93 0.7944986917176635 1101 94-95 1.0537519882453026 1101 96-97 0.9117519565513206 1101 98-99 0.8202184365259235 1101 100-101 0.45938485818989605 1102 1 0.1532095335335555 1102 2 0.17066353957938674 1102 3 0.1288123067415512 1102 4 0.10629950600615956 1102 5 0.17781173508512182 1102 6 0.15464663598996253 1102 7 0.1845673993892447 1102 8 0.23030812252488886 1102 9 0.2973197653248789 1102 10-11 0.2543417822237686 1102 12-13 0.26765433638028213 1102 14-15 0.31911321768277645 1102 16-17 0.4004105305545309 1102 18-19 0.20519289195118517 1102 20-21 0.2982425069392036 1102 22-23 0.22112808554393837 1102 24-25 0.20991215571475408 1102 26-27 0.21154410375860522 1102 28-29 0.28894914543685246 1102 30-31 0.2644178664181922 1102 32-33 0.25239470942621267 1102 34-35 0.315325738631266 1102 36-37 0.35433581475276554 1102 38-39 0.3560554643313836 1102 40-41 0.41050911618993524 1102 42-43 0.3414801851216325 1102 44-45 0.4381953772562497 1102 46-47 0.4462297760973044 1102 48-49 0.30647030770376205 1102 50-51 0.280130981925538 1102 52-53 0.3969792437042585 1102 54-55 0.36205036988641126 1102 56-57 0.30681959108215295 1102 58-59 0.37439295653614835 1102 60-61 0.37110286420910654 1102 62-63 0.35870371217235686 1102 64-65 0.355836156212213 1102 66-67 0.23833814829607292 1102 68-69 0.4395219534234016 1102 70-71 0.3020036490354343 1102 72-73 0.4958566668483826 1102 74-75 0.43764483734141635 1102 76-77 0.5954839471671534 1102 78-79 0.6262153573367542 1102 80-81 0.6319780940265431 1102 82-83 0.4439164510403586 1102 84-85 0.5604474454668171 1102 86-87 0.5069039256448775 1102 88-89 0.5940436495313719 1102 90-91 0.7629224172989204 1102 92-93 0.7246997080064155 1102 94-95 0.765474306313731 1102 96-97 0.5225314732759294 1102 98-99 0.5833861265621572 1102 100-101 0.388297691885402 1103 1 0.10682298895618914 1103 2 0.16455695709146312 1103 3 0.1806835520391985 1103 4 0.12616184025337418 1103 5 0.14497929539800225 1103 6 0.1194037279832898 1103 7 0.17477952624842175 1103 8 0.10834917848625025 1103 9 0.2130277270115002 1103 10-11 0.15732146177230533 1103 12-13 0.21307291716279764 1103 14-15 0.24892757785749353 1103 16-17 0.23486115695394005 1103 18-19 0.23153492598220993 1103 20-21 0.28991151478013677 1103 22-23 0.2795950305162265 1103 24-25 0.3065771823829593 1103 26-27 0.25669241420725086 1103 28-29 0.2898666739850597 1103 30-31 0.2871245509295406 1103 32-33 0.32902022554337407 1103 34-35 0.4556816065316269 1103 36-37 0.5565293891588183 1103 38-39 0.42935580710753385 1103 40-41 0.5458358711765214 1103 42-43 0.5671405609706852 1103 44-45 0.567877314026191 1103 46-47 0.5832955294471844 1103 48-49 0.5401829112683671 1103 50-51 0.6528679252319449 1103 52-53 0.6782951554069285 1103 54-55 0.5978759714623081 1103 56-57 0.6854648229454554 1103 58-59 0.574964297174887 1103 60-61 0.42207734564488675 1103 62-63 0.29951070905988786 1103 64-65 0.44502442521148566 1103 66-67 0.5882867601091419 1103 68-69 0.437683543388971 1103 70-71 0.5360756640264768 1103 72-73 0.5955398671465417 1103 74-75 0.5192670546381493 1103 76-77 0.7153477167071358 1103 78-79 0.6582316213214483 1103 80-81 0.6899916864843405 1103 82-83 0.6996257986080323 1103 84-85 0.5674039683312664 1103 86-87 0.5476590637576848 1103 88-89 0.6925533803457427 1103 90-91 0.7759323462307499 1103 92-93 0.5687806194596767 1103 94-95 0.7181099979507302 1103 96-97 0.7234605782838308 1103 98-99 0.5447551040972378 1103 100-101 0.41761537017464434 1104 1 0.13169892719580645 1104 2 0.134968654012539 1104 3 0.18520160672879626 1104 4 0.11787169395478969 1104 5 0.1180204806715821 1104 6 0.1521363954835664 1104 7 0.1665370409299527 1104 8 0.19312912567880147 1104 9 0.20608572194614538 1104 10-11 0.17763048136074389 1104 12-13 0.1789807660325735 1104 14-15 0.2355640577091549 1104 16-17 0.26567581371354265 1104 18-19 0.21644948107268647 1104 20-21 0.20445491915353387 1104 22-23 0.25082590053304443 1104 24-25 0.16302511723039714 1104 26-27 0.18665025027959814 1104 28-29 0.2649643274379443 1104 30-31 0.2360749438691201 1104 32-33 0.27524288664378815 1104 34-35 0.2503283160794254 1104 36-37 0.2513411248502706 1104 38-39 0.31495244123411936 1104 40-41 0.2910883907352755 1104 42-43 0.3289137396166453 1104 44-45 0.399647630721951 1104 46-47 0.37962629848230023 1104 48-49 0.3705940307583404 1104 50-51 0.3310390473982636 1104 52-53 0.2900639918626311 1104 54-55 0.23163599659431355 1104 56-57 0.4411105295504214 1104 58-59 0.5261533813439527 1104 60-61 0.4108818272435073 1104 62-63 0.28793682434401546 1104 64-65 0.3699019559694321 1104 66-67 0.31354836275085063 1104 68-69 0.24590051404332058 1104 70-71 0.22874592084812662 1104 72-73 0.2575175411853081 1104 74-75 0.09502887988870867 1104 76-77 0.35085848087075533 1104 78-79 0.43939159104755987 1104 80-81 0.3241725832760878 1104 82-83 0.4324998107024598 1104 84-85 0.3699598227608867 1104 86-87 0.3476169364923294 1104 88-89 0.6547027141116928 1104 90-91 0.6084483968677894 1104 92-93 0.5722959604522195 1104 94-95 0.6027216193933107 1104 96-97 0.5658831813203378 1104 98-99 0.5138282836368369 1104 100-101 0.32057835936133117 1105 1 0.04942464911626132 1105 2 0.0651419202237733 1105 3 0.1100974905959049 1105 4 0.09348359990962507 1105 5 0.12436222017315401 1105 6 0.0727655723895353 1105 7 0.02624005479707847 1105 8 0.09639987003956207 1105 9 0.10713231772636078 1105 10-11 0.09997449272802328 1105 12-13 0.02793251340747105 1105 14-15 0.11268879559629852 1105 16-17 0.050668046424689805 1105 18-19 0.0858297945742521 1105 20-21 0.15486455748418138 1105 22-23 0.14751897067988295 1105 24-25 0.18401833609564022 1105 26-27 0.21209806231651385 1105 28-29 0.11190698469903992 1105 30-31 0.21568448517492556 1105 32-33 0.1910774858066162 1105 34-35 0.2654095810075958 1105 36-37 0.17377526857957548 1105 38-39 0.24278591109147385 1105 40-41 0.2731199079015241 1105 42-43 0.24383376040010063 1105 44-45 0.2873165294900488 1105 46-47 0.3692445084005769 1105 48-49 0.43513841166831213 1105 50-51 0.42889131250404944 1105 52-53 0.3285803336551467 1105 54-55 0.3045809124663563 1105 56-57 0.24551733931546948 1105 58-59 0.14762927226258427 1105 60-61 0.0898790649517025 1105 62-63 0.1528257627173346 1105 64-65 0.10275195451141172 1105 66-67 0.2584453467629544 1105 68-69 0.3094839249169965 1105 70-71 0.40164133019485604 1105 72-73 0.31222689082729715 1105 74-75 0.32331228254443545 1105 76-77 0.31651341177369474 1105 78-79 0.3595591709671311 1105 80-81 0.400872572407188 1105 82-83 0.31262078688896366 1105 84-85 0.28105021226523874 1105 86-87 0.2713367918563989 1105 88-89 0.40106395136393047 1105 90-91 0.48379467744983273 1105 92-93 0.36072647767943167 1105 94-95 0.37339142213598464 1105 96-97 0.23391906460422973 1105 98-99 0.4581765206213255 1105 100-101 0.3720906611032717 1106 1 0.09843344281666333 1106 2 0.0546540792253154 1106 3 0.09263392674709792 1106 4 0.055719712297403134 1106 5 0.08944658250220527 1106 6 0.07549892206424147 1106 7 0.06738073357679042 1106 8 0.08317612602714064 1106 9 0.130431538447219 1106 10-11 0.1337743211436262 1106 12-13 0.1728772846793376 1106 14-15 0.16450690081430253 1106 16-17 0.09552766383234257 1106 18-19 0.05682896729233278 1106 20-21 0.11076519684033315 1106 22-23 0.1864899494258836 1106 24-25 0.1641546333451842 1106 26-27 0.27915066621189055 1106 28-29 0.33615081327805285 1106 30-31 0.2945660715135361 1106 32-33 0.26366892088541505 1106 34-35 0.25321547105060205 1106 36-37 0.2425373331075633 1106 38-39 0.3077620118360258 1106 40-41 0.3401303816991472 1106 42-43 0.32454417598160035 1106 44-45 0.3601447867849359 1106 46-47 0.35757766300015703 1106 48-49 0.22309826806850452 1106 50-51 0.3181912890133276 1106 52-53 0.2318087758118068 1106 54-55 0.2780670376208363 1106 56-57 0.3414443801995404 1106 58-59 0.2774710418288251 1106 60-61 0.17578552592136276 1106 62-63 0.3693412716999802 1106 64-65 0.3702148800253937 1106 66-67 0.41794606680463176 1106 68-69 0.5279578451098317 1106 70-71 0.42982253557524785 1106 72-73 0.44074156231467043 1106 74-75 0.5261514681313457 1106 76-77 0.46517584500387343 1106 78-79 0.6754110670768618 1106 80-81 0.7030615501522846 1106 82-83 0.6453694055324206 1106 84-85 0.48468986970924277 1106 86-87 0.5616693387612415 1106 88-89 0.7997142562326331 1106 90-91 0.8295212812022221 1106 92-93 0.6046074001498383 1106 94-95 0.6311567651515837 1106 96-97 0.646631885431308 1106 98-99 0.8233238227630686 1106 100-101 0.7710856667716861 1107 1 -0.013034337255767525 1107 2 -0.008527457793434223 1107 3 -0.021910434890287434 1107 4 -0.07006221581010408 1107 5 -0.022394078757059788 1107 6 -0.005498603700729632 1107 7 -0.019498088767150534 1107 8 0.059099724043761626 1107 9 -0.008838961288397229 1107 10-11 -0.00987971495226958 1107 12-13 -0.017724238771016587 1107 14-15 0.019418082760367383 1107 16-17 -0.01079834656343337 1107 18-19 0.05547462291472982 1107 20-21 0.052600096524059836 1107 22-23 0.014341679893895787 1107 24-25 -0.033387593266816395 1107 26-27 0.046722848028252884 1107 28-29 -0.0029222714345635836 1107 30-31 0.04411462338767791 1107 32-33 -0.08733573825877983 1107 34-35 0.129243700276497 1107 36-37 0.10323627956232428 1107 38-39 0.049864163821901286 1107 40-41 0.09987579987480188 1107 42-43 0.04935349526766686 1107 44-45 0.11465615030792975 1107 46-47 0.09255703606093135 1107 48-49 0.16458772263936083 1107 50-51 0.13745864607138003 1107 52-53 0.17686679063725563 1107 54-55 0.15084720981960942 1107 56-57 0.19467697325771383 1107 58-59 0.1764221108380255 1107 60-61 0.10286480619831906 1107 62-63 0.18686643806235992 1107 64-65 0.0959882031824364 1107 66-67 0.10596049319114798 1107 68-69 0.1709621450908756 1107 70-71 0.13671173374351753 1107 72-73 0.13087480186651135 1107 74-75 0.10403562305037894 1107 76-77 0.19894927790521422 1107 78-79 0.4273690050813137 1107 80-81 0.2655097957400656 1107 82-83 0.3904932669353549 1107 84-85 0.37698394950332315 1107 86-87 0.31012169477173757 1107 88-89 0.5561043672739103 1107 90-91 0.550732733518327 1107 92-93 0.5540876112579554 1107 94-95 0.6318694795270865 1107 96-97 0.5606921250729435 1107 98-99 0.6600411463989957 1107 100-101 0.5574659008263367 1108 1 0.032805153028164113 1108 2 0.06401364252544539 1108 3 0.10592283183186879 1108 4 0.04997937645370598 1108 5 0.09944845975214633 1108 6 0.13560128739916166 1108 7 0.07233428084299476 1108 8 0.1112373317369233 1108 9 0.13115115092394092 1108 10-11 0.1723536478366512 1108 12-13 0.09119160625593992 1108 14-15 0.17569264181270228 1108 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3540.0 12 4786.0 13 6953.0 14 8798.0 15 11463.0 16 14737.0 17 19472.0 18 18998.0 19 15257.0 20 15351.0 21 16799.0 22 18033.0 23 19937.0 24 23390.0 25 28114.0 26 33965.0 27 41381.0 28 50464.0 29 62234.0 30 76022.0 31 92937.0 32 115170.0 33 148561.0 34 200353.0 35 285005.0 36 451693.0 37 734463.0 38 586647.0 39 45235.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.14655384215396 19.211739207683575 15.591580179916173 23.05012677024629 2 15.570576805429203 20.050621435975135 39.55359944718983 24.825202311405832 3 19.663369035656892 23.649783345325677 29.19655507973589 27.490292539281537 4 13.591078962466598 16.61172375974312 36.162248517333964 33.634948760456325 5 14.53123662747758 37.041102577350635 33.50435369707651 14.923307098095279 6 33.079475971459274 35.5391008593282 16.886860932106416 14.494562237106114 7 29.940598264860736 31.837459862621603 19.721312670573955 18.50062920194371 8 26.95542334038082 35.26307614769921 18.87279343474526 18.908707077174707 9 26.318011658462208 17.422712763765574 19.67519232658909 36.58408325118312 10-11 25.65069307307301 26.402565622434455 26.44000076074794 21.50674054374459 12-13 26.514078591601976 23.149290444054913 27.175152704300444 23.161478260042664 14-15 23.141904234045597 24.850576195560816 25.304961499526975 26.702558070866612 16-17 23.046004109646574 28.175787636033977 25.834747196509415 22.943461057810033 18-19 23.09405697368129 27.05593716221999 27.610363922796765 22.239641941301958 20-21 23.843789919455812 25.994725480935337 27.558331933345805 22.60315266626305 22-23 23.467806098028714 25.775487433394932 27.511403294672544 23.245303173903807 24-25 23.417620005648555 26.052376244496863 27.55640710044317 22.97359664941142 26-27 23.22746171366751 26.22476878901757 27.57091413150684 22.97685536580808 28-29 23.00761698876946 26.578583677518946 27.567254872749057 22.846544460962537 30-31 23.237378716174454 26.13706459067006 27.37858938312851 23.246967310026974 32-33 23.017744445747578 26.23011357332818 27.397845815410854 23.354296165513393 34-35 23.593218125425658 26.179650604095794 27.27054004162396 22.956591228854588 36-37 23.615692624102707 25.94861691720843 27.392168018359413 23.043522440329454 38-39 23.288511289569445 26.28237824170745 27.380649341633305 23.0484611270898 40-41 23.48663709567224 26.102135648878942 27.29983384148903 23.11139341395979 42-43 23.304646794514262 26.103244595102005 27.07994048720042 23.512168123183315 44-45 23.359694531728692 26.15039544967403 27.096044932693836 23.393865085903442 46-47 23.633214883615665 26.370707729798976 26.806220836954157 23.1898565496312 48-49 23.599970204038936 26.318185996532257 26.724187663838162 23.35765613559064 50-51 23.35844858136358 26.525790940125965 26.820184544771603 23.295575933738853 52-53 23.70463644172829 26.604607596702156 26.421964694955925 23.268791266613626 54-55 23.37287109443101 26.29089416411235 26.64253405139486 23.693700690061778 56-57 23.467108745748526 26.45917794845299 26.676419032645597 23.397294273152887 58-59 23.741544350546054 26.185003373463577 26.663897314843442 23.409554961146924 60-61 23.41031346779894 26.293034858988623 27.031200966174385 23.26545070703805 62-63 23.310373727689747 26.37360216448659 26.986187625079506 23.32983648274416 64-65 23.211988753609592 26.42041150124097 26.82734825455894 23.5402514905905 66-67 23.196409269713676 26.45619835234675 26.590834889168534 23.756557488771044 68-69 23.137356584258665 26.323578846702162 26.87304829305333 23.666016275985843 70-71 23.361222141568852 26.26030575727703 26.654452435819813 23.7240196653343 72-73 23.351554303139036 26.13914079859515 26.748594993644588 23.760709904621226 74-75 23.147451969861702 26.66315349040665 26.4762947771484 23.713099762583244 76-77 23.31412843995891 26.224409250564367 26.611933064732757 23.849529244743962 78-79 23.297969119973118 26.346349519302393 26.596857477042846 23.75882388368164 80-81 23.390540764355944 26.527336973878256 26.518810142491596 23.563312119274205 82-83 23.309095058625136 26.395703042040836 26.671711904754357 23.62348999457967 84-85 23.27749232120046 26.34005749986528 26.59200770891248 23.790442470021777 86-87 23.285101932608885 26.333830761630097 26.63649202945986 23.744575276301163 88-89 23.17938370417215 26.31762948607977 26.48412800535951 24.018858804388575 90-91 23.16405414965848 26.23968374950375 26.640372899213126 23.955889201624643 92-93 23.292342754985278 26.181235518053498 26.645275913769222 23.881145813192003 94-95 23.03915998198929 26.097430589267525 26.706567902549438 24.156841526193745 96-97 22.92838826039134 26.034411165243966 26.833339144602338 24.20386142976236 98-99 22.98664887361758 26.1906022270896 26.439731329185147 24.383017570107675 100-101 23.048351058282652 25.685142645400745 26.989337770197043 24.277168526119564 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 97.0 1 73.5 2 65.0 3 105.0 4 114.5 5 102.0 6 197.0 7 512.0 8 847.5 9 1027.0 10 1044.0 11 1018.0 12 1146.5 13 1346.5 14 1530.5 15 1705.0 16 1834.0 17 1967.0 18 2132.0 19 2302.0 20 2609.0 21 3060.5 22 3732.5 23 4601.5 24 5582.0 25 7097.5 26 9561.0 27 13253.5 28 16630.5 29 20368.0 30 25751.0 31 30705.5 32 36243.0 33 41996.5 34 47534.5 35 53806.0 36 61704.5 37 69911.5 38 75052.0 39 79251.5 40 84924.5 41 91119.0 42 96761.5 43 102532.0 44 109513.5 45 116235.5 46 119948.0 47 126867.0 48 142557.0 49 144744.0 50 133323.5 51 127003.0 52 123570.5 53 122123.0 54 121160.0 55 115263.5 56 105234.5 57 96585.0 58 87629.0 59 76567.5 60 64042.5 61 52481.5 62 42405.5 63 32153.0 64 24043.5 65 18294.5 66 13484.5 67 10399.5 68 7527.5 69 5062.5 70 3721.5 71 2653.5 72 1949.5 73 1542.0 74 1225.0 75 980.5 76 665.0 77 367.0 78 225.5 79 136.0 80 81.5 81 49.5 82 28.0 83 20.5 84 11.5 85 4.0 86 1.0 87 0.5 88 1.5 89 1.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.27092136085127694 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0028052580361925834 16-17 5.547120410550306E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 3.169783091743032E-5 26-27 0.0013154599830733583 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0036927973018806323 36-37 0.020873021659127865 38-39 0.0076391772511007075 40-41 0.01816285711568757 42-43 0.005531271495091591 44-45 8.558414347706187E-4 46-47 1.7433807004586677E-4 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 6.498055338073216E-4 60-61 0.002535826473394426 62-63 0.00147394913766051 64-65 0.0 66-67 0.0 68-69 6.181077028898912E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.22953984258857166 78-79 0.0 80-81 0.0013471578139907885 82-83 0.0 84-85 0.0 86-87 0.10292285698889625 88-89 0.35560211614719206 90-91 0.35520589326072416 92-93 0.0 94-95 1.584891545871516E-5 96-97 0.0 98-99 0.0 100-101 2.8528047825687286E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3154790.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.07489484232882 #Duplication Level Percentage of deduplicated Percentage of total 1 69.82067612535381 33.56621662546683 2 15.394399462946067 14.80168270683871 3 5.877070099812446 8.476185810884347 4 2.861658033551005 5.502956361506801 5 1.6061736386616146 3.8608314388588876 6 0.9914402232120781 2.8598030684025404 7 0.6627677626891162 2.2303743347315357 8 0.4756077349659091 1.8291833475747417 9 0.34633143270883887 1.4984862487263457 >10 1.8013968887729275 15.390086757535867 >50 0.11060812784482427 3.62523296606757 >100 0.04758447084979829 4.01212638117685 >500 0.0025715991799342944 0.802671545595596 >1k 0.001582522570729531 1.1930550241430686 >5k 1.318768808941276E-4 0.35110738249039825 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5774 0.18302327571724267 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5302 0.16806189952421557 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4105 0.13011959591605146 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 6.339566183486064E-5 7 0.0 0.0 0.0 0.0 6.339566183486064E-5 8 0.0 0.0 0.0 0.0 9.509349275229096E-5 9 0.0 0.0 0.0 0.0 1.2679132366972127E-4 10-11 0.0 0.0 0.0 0.0 1.2679132366972127E-4 12-13 0.0 0.0 0.0 0.0 1.2679132366972127E-4 14-15 0.0 0.0 0.0 0.0 1.2679132366972127E-4 16-17 0.0 0.0 0.0 1.584891545871516E-5 1.2679132366972127E-4 18-19 0.0 0.0 0.0 6.339566183486064E-5 1.2679132366972127E-4 20-21 0.0 0.0 0.0 7.92445772935758E-5 1.2679132366972127E-4 22-23 0.0 0.0 0.0 2.2188481642201224E-4 1.7433807004586677E-4 24-25 0.0 0.0 0.0 2.377337318807274E-4 1.9018698550458192E-4 26-27 1.584891545871516E-5 0.0 0.0 3.169783091743032E-4 1.9018698550458192E-4 28-29 3.169783091743032E-5 3.169783091743032E-5 0.0 9.350860120641944E-4 1.9018698550458192E-4 30-31 3.169783091743032E-5 3.169783091743032E-5 0.0 0.0034075168236237592 2.0603590096329708E-4 32-33 3.169783091743032E-5 3.169783091743032E-5 0.0 0.009683687345274961 2.2188481642201224E-4 34-35 3.169783091743032E-5 3.169783091743032E-5 0.0 0.017766634229219695 2.2188481642201224E-4 36-37 3.169783091743032E-5 3.169783091743032E-5 0.0 0.032997441985044965 2.377337318807274E-4 38-39 3.169783091743032E-5 3.169783091743032E-5 0.0 0.05824476431077821 2.5358264733944255E-4 40-41 3.169783091743032E-5 3.169783091743032E-5 0.0 0.08758110682485998 2.5358264733944255E-4 42-43 3.169783091743032E-5 3.169783091743032E-5 0.0 0.1193898801505013 2.694315627981577E-4 44-45 3.169783091743032E-5 3.169783091743032E-5 0.0 0.1592340536137112 3.0112939371558804E-4 46-47 3.169783091743032E-5 3.169783091743032E-5 0.0 0.20804871322655388 3.328272246330184E-4 48-49 3.169783091743032E-5 3.169783091743032E-5 0.0 0.26545348501802024 3.4867614009173354E-4 50-51 3.169783091743032E-5 3.169783091743032E-5 0.0 0.32884914685288086 3.4867614009173354E-4 52-53 3.169783091743032E-5 3.169783091743032E-5 0.0 0.39550968527223684 3.4867614009173354E-4 54-55 3.169783091743032E-5 3.169783091743032E-5 0.0 0.46817696265044584 3.4867614009173354E-4 56-57 3.169783091743032E-5 3.169783091743032E-5 0.0 0.5584365361878286 3.4867614009173354E-4 58-59 3.169783091743032E-5 3.169783091743032E-5 0.0 0.6592990341670919 3.4867614009173354E-4 60-61 3.169783091743032E-5 3.169783091743032E-5 0.0 0.774140275580942 3.4867614009173354E-4 62-63 3.169783091743032E-5 3.169783091743032E-5 0.0 0.8919928109319479 3.4867614009173354E-4 64-65 3.169783091743032E-5 3.169783091743032E-5 0.0 1.0143305893577703 3.4867614009173354E-4 66-67 3.169783091743032E-5 3.169783091743032E-5 0.0 1.1599821224233624 3.4867614009173354E-4 68-69 3.169783091743032E-5 3.169783091743032E-5 0.0 1.326601770640835 3.4867614009173354E-4 70-71 3.169783091743032E-5 3.169783091743032E-5 0.0 1.5126997359570684 3.8037397100916385E-4 72-73 3.169783091743032E-5 3.169783091743032E-5 0.0 1.707308568874632 3.8037397100916385E-4 74-75 3.169783091743032E-5 3.169783091743032E-5 0.0 1.9168946269006812 3.8037397100916385E-4 76-77 3.169783091743032E-5 3.169783091743032E-5 0.0 2.139920565235721 3.8037397100916385E-4 78-79 3.169783091743032E-5 3.169783091743032E-5 0.0 2.4032344466668145 3.8037397100916385E-4 80-81 3.169783091743032E-5 3.169783091743032E-5 0.0 2.6972952240878154 4.279207173853093E-4 82-83 3.169783091743032E-5 3.169783091743032E-5 0.0 3.0079973627404675 5.071652946788851E-4 84-85 3.169783091743032E-5 3.169783091743032E-5 0.0 3.3263228297287615 5.071652946788851E-4 86-87 3.169783091743032E-5 3.169783091743032E-5 0.0 3.658753831475312 5.071652946788851E-4 88-89 3.169783091743032E-5 3.169783091743032E-5 0.0 4.034151243030439 5.071652946788851E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 12350 0.0 21.614422 1 TACATGG 12295 0.0 21.226364 2 ACATGGG 12175 0.0 20.53918 3 GTATCAA 18410 0.0 20.366674 1 GAGTACT 7155 0.0 19.59885 12-13 ACCGTAT 195 1.0429123E-5 19.466902 8 AGTACTT 7805 0.0 19.213078 12-13 CATGGGG 7325 0.0 18.98023 4 GTACTTT 7745 0.0 18.16712 14-15 CGCACCG 240 3.1244563E-6 17.793964 5 AGAGTAC 16060 0.0 17.714258 10-11 TATCAAC 21240 0.0 17.581736 2 ATCAACG 21085 0.0 17.575954 3 TCAACGC 21085 0.0 17.575954 4 TAAGGTG 1350 0.0 17.574287 5 CAACGCA 21230 0.0 17.455912 5 AACGCAG 21750 0.0 17.038574 6 TACACTG 2780 0.0 16.897865 5 GTGTAGC 1710 0.0 16.695633 1 CCATATA 1340 0.0 16.643112 2 >>END_MODULE