FastQCFastQC Report
Fri 27 May 2016
SRR939293_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939293_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1728284
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT120910.6995956683045148No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86340.49957067241263586No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT64490.3731446914974622No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63330.366432831641096No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT50960.2948589467934668No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47150.2728139588169537No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC42710.24712373660810377No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT33510.1938917446438201No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT30220.17485552143050562No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC29900.17300397388392186No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC28350.16403554045515667No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG26560.15367844636645367No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC24550.1420484133394743No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA22670.13117057150329461No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA18780.10866269664013553No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA18540.1072740359801977No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG459.0437644E-426.34242218-19
GTACATG95500.026.0380781
TACATGG96100.025.0643232
CATGGGG34500.023.363234
ACATGGG97900.023.3442543
GCGCCGT753.012894E-522.1881294-95
GAGTACG1954.2627835E-721.902261
GAGTACT69900.021.48797812-13
AGTACTT75100.021.35660612-13
GTACTTT75400.019.98715814-15
CATTCCG2852.0263542E-919.9514759
TAGGACA9500.018.9654454
CATGGGT12050.018.886754
CATGGGA57750.018.7191414
ATGGGTA7400.017.9402855
AGAGTAC144850.017.81118610-11
CTTTGCG2403.1452255E-617.780622
ATACGAT2152.4151897E-517.6576351
GTATAGG7000.017.6261041
AGTACAT71400.017.5315912
Produced by FastQC (version 0.11.5)