FastQCFastQC Report
Fri 27 May 2016
SRR939293_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939293_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1728284
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT123480.7144659095380157No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84490.4888664131589484No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63540.3676479097185416No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT62620.36232471052211324No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT51000.2950903902367898No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46650.26992091577541655No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC43420.2512318577270865No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT32720.1893207366381914No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT31690.18336106797262486No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC30630.1772278167245661No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC29870.1728303913014296No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG28010.16206827118691142No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC25760.1490495774999942No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA24090.1393868137412601No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA19290.11161360054250344No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA18720.10831553147515108No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACGG300.00396759831.64125832-33
GTATAGG6500.025.6246221
GTACATG100100.025.3869151
TACATGG100750.024.7269942
ACATGGG100750.023.7850133
TACGGTG1801.971257E-723.726145
CATGGGG33250.021.8351844
GAGTACT78400.021.45890412-13
GTAAGCG1900.021.22865394-95
AGTACTT83750.020.73975412-13
GTACTTT83700.020.10009414-15
TTACGGT1908.327213E-619.9799084
TCGTATC600.004793187619.77178494-95
CATGGGT14500.019.6354264
CATGGGA58500.019.6298334
CGTATAG1801.2162801E-418.5066721
TACTTTT96750.017.87943814-15
GTACACG1901.839959E-417.5326371
ATGGGCG2453.8001526E-617.431455
TATAGGG3852.5465852E-1017.2553732
Produced by FastQC (version 0.11.5)