##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939293_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1728284 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.274211877214626 33.0 31.0 34.0 31.0 34.0 2 32.46702740984699 34.0 31.0 34.0 31.0 34.0 3 32.55673430987037 34.0 31.0 34.0 31.0 34.0 4 36.0895414179614 37.0 35.0 37.0 35.0 37.0 5 36.039770662692014 37.0 35.0 37.0 35.0 37.0 6 36.078668783602694 37.0 35.0 37.0 35.0 37.0 7 36.02205366710564 37.0 35.0 37.0 35.0 37.0 8 36.00250248223093 37.0 35.0 37.0 35.0 37.0 9 37.68597695749078 39.0 37.0 39.0 35.0 39.0 10-11 37.60857445882737 39.0 37.0 39.0 35.0 39.0 12-13 37.546542987147944 39.0 37.0 39.0 35.0 39.0 14-15 38.791566374507894 40.0 38.0 41.0 34.5 41.0 16-17 38.813672116388275 40.0 38.0 41.0 34.5 41.0 18-19 38.951916756736736 40.0 38.0 41.0 35.0 41.0 20-21 38.944775279988704 40.0 38.0 41.0 35.0 41.0 22-23 38.86471581059594 40.0 38.0 41.0 35.0 41.0 24-25 38.794689125166926 40.0 38.0 41.0 34.0 41.0 26-27 38.63018838339069 40.0 38.0 41.0 34.0 41.0 28-29 38.47539090797577 40.0 38.0 41.0 34.0 41.0 30-31 38.28212029967297 40.0 38.0 41.0 34.0 41.0 32-33 38.18033407703827 40.0 38.0 41.0 34.0 41.0 34-35 38.061520270973986 40.0 37.0 41.0 33.0 41.0 36-37 37.892141858629714 40.0 37.0 41.0 33.0 41.0 38-39 37.74614530945146 40.0 37.0 41.0 33.0 41.0 40-41 37.58469180991087 40.0 37.0 41.0 33.0 41.0 42-43 37.37749814266637 40.0 37.0 41.0 32.5 41.0 44-45 37.092964177183845 40.0 37.0 41.0 31.5 41.0 46-47 36.743889893096274 40.0 36.0 41.0 31.0 41.0 48-49 36.39557705793724 40.0 36.0 41.0 30.0 41.0 50-51 36.153890217117095 39.0 36.0 41.0 29.5 41.0 52-53 35.93069570741845 39.0 35.0 41.0 29.0 41.0 54-55 35.70803554276959 39.0 35.0 40.0 28.0 41.0 56-57 35.40723457487311 38.5 35.0 40.0 27.5 41.0 58-59 35.11235798051709 38.0 35.0 40.0 27.0 41.0 60-61 34.77018678643093 38.0 34.0 40.0 26.0 41.0 62-63 34.46473380532366 38.0 34.0 40.0 26.0 41.0 64-65 34.197080167379895 37.0 34.0 40.0 26.0 41.0 66-67 33.78993122658082 37.0 33.5 39.0 25.0 41.0 68-69 33.328822693492505 36.0 33.0 39.0 23.5 40.0 70-71 32.84956262975298 35.5 32.5 38.5 22.5 40.0 72-73 32.36602549118085 35.0 32.0 38.0 21.5 40.0 74-75 31.856860330825256 35.0 31.5 37.0 20.0 39.0 76-77 29.878898375498473 33.5 29.5 35.5 15.5 37.5 78-79 30.819328883447398 34.0 30.5 36.0 17.5 38.5 80-81 30.81055110155507 34.0 31.0 36.0 17.5 37.5 82-83 30.451473253238472 34.0 31.0 35.5 12.0 37.0 84-85 30.00213332993883 34.0 30.0 35.0 7.0 36.5 86-87 29.455078274172532 34.0 29.5 35.0 2.0 36.0 88-89 28.8709046082704 34.0 29.0 35.0 2.0 36.0 90-91 28.40237773421498 34.0 29.0 35.0 2.0 35.0 92-93 28.07516646569661 33.0 29.0 35.0 2.0 35.0 94-95 27.59637825727716 33.0 27.0 35.0 2.0 35.0 96-97 26.906672456610142 33.0 26.0 35.0 2.0 35.0 98-99 25.996053889291343 32.5 24.5 34.0 2.0 35.0 100-101 21.83101504150938 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2866893993680435 1101 2 0.33947056722900015 1101 3 0.23353648549770156 1101 4 0.15154787803454894 1101 5 0.23635060418941833 1101 6 0.24041359264697348 1101 7 0.22685088939992681 1101 8 0.22743316923833135 1101 9 0.30671226006445096 1101 10-11 0.3306880485958388 1101 12-13 0.26589001867532147 1101 14-15 0.39687023374517594 1101 16-17 0.31622888950734307 1101 18-19 0.33484291829376645 1101 20-21 0.3596459186592895 1101 22-23 0.2750237780549867 1101 24-25 0.23919065449241117 1101 26-27 0.32996794773349336 1101 28-29 0.3173059460673002 1101 30-31 0.18686991644214856 1101 32-33 0.4351827230215548 1101 34-35 0.42726965540569495 1101 36-37 0.3281706632072243 1101 38-39 0.4436865115314603 1101 40-41 0.45794396332738785 1101 42-43 0.5493756777126606 1101 44-45 0.5856432443900133 1101 46-47 0.5871823871445372 1101 48-49 0.4598455317900445 1101 50-51 0.5589005912533693 1101 52-53 0.5607151828798393 1101 54-55 0.5073748412290087 1101 56-57 0.5414007102541305 1101 58-59 0.4726918982022852 1101 60-61 0.40569355614734803 1101 62-63 0.3816822014966661 1101 64-65 0.40879487624602007 1101 66-67 0.3109436578156135 1101 68-69 0.5109438581426318 1101 70-71 0.4926265197712567 1101 72-73 0.4996787806139551 1101 74-75 0.6229426291734406 1101 76-77 0.607328858329641 1101 78-79 0.6460087945433912 1101 80-81 0.6467958442498265 1101 82-83 0.5299583475129559 1101 84-85 0.4505477213001754 1101 86-87 0.3138823595869944 1101 88-89 0.40747035319225233 1101 90-91 0.502757711390931 1101 92-93 0.513227739478257 1101 94-95 0.5737387781166312 1101 96-97 0.5905094226997072 1101 98-99 0.330900061563856 1101 100-101 0.5224009799347087 1102 1 0.27036829628822545 1102 2 0.30110672373434255 1102 3 0.2465004232726855 1102 4 0.10577988620551082 1102 5 0.11601315918628075 1102 6 0.23167184217557235 1102 7 0.23721622566642964 1102 8 0.31546113906862416 1102 9 0.3022559432825531 1102 10-11 0.27998566016089654 1102 12-13 0.34559991025295034 1102 14-15 0.363816140407657 1102 16-17 0.37167102495673987 1102 18-19 0.2809194817758609 1102 20-21 0.30253384292075935 1102 22-23 0.33414697101539304 1102 24-25 0.29169556492610127 1102 26-27 0.24445785188183322 1102 28-29 0.3571731679403385 1102 30-31 0.18439289727181318 1102 32-33 0.34444733647221426 1102 34-35 0.35655096433089284 1102 36-37 0.32897754567108706 1102 38-39 0.31401158224170445 1102 40-41 0.3353905378088413 1102 42-43 0.26334664722869405 1102 44-45 0.2423226127118241 1102 46-47 0.44513709330419005 1102 48-49 0.5173321754104094 1102 50-51 0.43882654191333614 1102 52-53 0.3393803305856906 1102 54-55 0.4191846306696121 1102 56-57 0.40277091767085693 1102 58-59 0.398557902287763 1102 60-61 0.4769296105156613 1102 62-63 0.6122814708241293 1102 64-65 0.652344334134142 1102 66-67 0.593366858632713 1102 68-69 0.38953417083904895 1102 70-71 0.3430317287593212 1102 72-73 0.43653335320980347 1102 74-75 0.594761060977337 1102 76-77 0.5119226672548471 1102 78-79 0.5966603108853121 1102 80-81 0.4568930708368768 1102 82-83 0.5455921862936002 1102 84-85 0.5375853548639711 1102 86-87 0.40948771785223315 1102 88-89 0.3726695203827006 1102 90-91 0.7204631639364969 1102 92-93 0.5689580191765593 1102 94-95 0.5099310314164427 1102 96-97 0.38235437084552615 1102 98-99 0.3193758629466359 1102 100-101 0.37890809024269245 1103 1 0.220962432090694 1103 2 0.23651567435162946 1103 3 0.18206369487761975 1103 4 0.15760858990921633 1103 5 0.20660729498874986 1103 6 0.10546042242248888 1103 7 0.22019462072815799 1103 8 0.21512163289578723 1103 9 0.2601571778504592 1103 10-11 0.17032516633373262 1103 12-13 0.191475681857888 1103 14-15 0.30638944287678527 1103 16-17 0.22939864841353597 1103 18-19 0.268515932393143 1103 20-21 0.2770670219331066 1103 22-23 0.325628452496872 1103 24-25 0.3064216451730104 1103 26-27 0.2902101666966388 1103 28-29 0.25481205682922337 1103 30-31 0.39867144665453225 1103 32-33 0.4516695586944408 1103 34-35 0.32326778531854927 1103 36-37 0.29167816295503 1103 38-39 0.36042747730343905 1103 40-41 0.2552362168211957 1103 42-43 0.24236285093238763 1103 44-45 0.42555563740317837 1103 46-47 0.6300560747856636 1103 48-49 0.6479031630647256 1103 50-51 0.6114770048762992 1103 52-53 0.388222923178283 1103 54-55 0.5068929640029438 1103 56-57 0.5766598065597393 1103 58-59 0.4976358343865357 1103 60-61 0.6303748265650526 1103 62-63 0.5493300819352385 1103 64-65 0.6688219575909287 1103 66-67 0.5445126919660481 1103 68-69 0.498295744913122 1103 70-71 0.5513920682654927 1103 72-73 0.5864793408641233 1103 74-75 0.35177494986622904 1103 76-77 0.5348084697239806 1103 78-79 0.5386857738482789 1103 80-81 0.7585250152813181 1103 82-83 0.7281346245652038 1103 84-85 0.6498307252343416 1103 86-87 0.5048349400744563 1103 88-89 0.6987420512468958 1103 90-91 0.8365048306031646 1103 92-93 0.6165120315222374 1103 94-95 0.5502010931448389 1103 96-97 0.6793026733146625 1103 98-99 0.12320302344046397 1103 100-101 -0.03127323691779793 1104 1 0.14732765211644505 1104 2 0.12536669305949744 1104 3 0.07681867480642524 1104 4 0.1290582604386401 1104 5 0.08235901808198776 1104 6 0.0435161673289457 1104 7 0.11490794345783684 1104 8 0.04747034152261165 1104 9 0.07201054450954558 1104 10-11 0.07647339022224742 1104 12-13 0.11475826914865195 1104 14-15 0.17590394715612234 1104 16-17 0.17720975195061328 1104 18-19 0.2118148038617491 1104 20-21 0.19002809138702048 1104 22-23 0.19890157224238436 1104 24-25 0.1532197213678188 1104 26-27 0.2881254132315263 1104 28-29 0.22665936425935485 1104 30-31 0.18666091874420232 1104 32-33 0.24493813401822706 1104 34-35 0.3949692925517496 1104 36-37 0.369844110088259 1104 38-39 0.3463984687447734 1104 40-41 0.4965715194039362 1104 42-43 0.6006223374127373 1104 44-45 0.6857313550431101 1104 46-47 0.43891201661707413 1104 48-49 0.595269291974823 1104 50-51 0.669526311851989 1104 52-53 0.6532990422421321 1104 54-55 0.6382835570499736 1104 56-57 0.5698261323947733 1104 58-59 0.6341464759074 1104 60-61 0.6760841350555395 1104 62-63 0.9185870689836335 1104 64-65 0.8319704844408946 1104 66-67 0.8248296653811806 1104 68-69 0.7903259653175709 1104 70-71 0.7527345661826317 1104 72-73 0.6080785986085786 1104 74-75 0.5460148953331618 1104 76-77 0.4219610108131313 1104 78-79 0.6660602341981949 1104 80-81 0.7435284235976134 1104 82-83 0.8536155758641506 1104 84-85 0.7616689438210251 1104 86-87 0.8206495276681842 1104 88-89 0.850035317928711 1104 90-91 0.9448381639364989 1104 92-93 0.8895446756182785 1104 94-95 0.8096894669992629 1104 96-97 0.7759644168577964 1104 98-99 0.5917991758300722 1104 100-101 0.6393432896291955 1105 1 0.11717435144409905 1105 2 0.09417177169597579 1105 3 0.1483484448554151 1105 4 0.12093126510239216 1105 5 0.1627712527114582 1105 6 0.15075307718756648 1105 7 0.14971322806450615 1105 8 0.17381245801346523 1105 9 0.23949814951756565 1105 10-11 0.27686215896185473 1105 12-13 0.22610575557668966 1105 14-15 0.1611745037170067 1105 16-17 0.18669725397353432 1105 18-19 0.24265884028904594 1105 20-21 0.2570474820094901 1105 22-23 0.14852346981634668 1105 24-25 0.24116274118508585 1105 26-27 0.39655842142619235 1105 28-29 0.24703827585400262 1105 30-31 0.1522863325236088 1105 32-33 0.24779266021322144 1105 34-35 0.3308772532997182 1105 36-37 0.29675632264950025 1105 38-39 0.3139268999875071 1105 40-41 0.43006080159781135 1105 42-43 0.46695650933900623 1105 44-45 0.4696292673880791 1105 46-47 0.3377832263977112 1105 48-49 0.3377668276885828 1105 50-51 0.2854444975488235 1105 52-53 0.39844807638905166 1105 54-55 0.3856699424202077 1105 56-57 0.4470634836180949 1105 58-59 0.3719519370599471 1105 60-61 0.4237978659113466 1105 62-63 0.5199935511598568 1105 64-65 0.42869850080081306 1105 66-67 0.4364791188245647 1105 68-69 0.368646131270701 1105 70-71 0.4979170704859328 1105 72-73 0.3856355714352233 1105 74-75 0.36468462212841146 1105 76-77 0.47226739147546937 1105 78-79 0.6051592617246406 1105 80-81 0.5426776931390371 1105 82-83 0.522836640730052 1105 84-85 0.602661793712894 1105 86-87 0.6255383773246557 1105 88-89 0.7905996762579974 1105 90-91 0.6886631039844566 1105 92-93 0.8210128752916681 1105 94-95 0.7481249762605664 1105 96-97 0.6502148324762231 1105 98-99 0.6212823377667789 1105 100-101 0.524544331249885 1106 1 0.08265850862097324 1106 2 0.10154702332712873 1106 3 0.09960942559961694 1106 4 0.06795375182505836 1106 5 0.02976352276975547 1106 6 0.07899477702141411 1106 7 0.00442317738254161 1106 8 -0.05915927977954283 1106 9 0.11540893921041828 1106 10-11 0.1194315589392616 1106 12-13 0.13956242479628855 1106 14-15 0.12064514709759067 1106 16-17 0.11443516835406342 1106 18-19 0.1405426275001176 1106 20-21 0.17002966171075684 1106 22-23 0.22851288142842208 1106 24-25 0.2336645512553588 1106 26-27 0.24044770790277425 1106 28-29 0.2905014111049624 1106 30-31 0.19473666545098922 1106 32-33 0.2363845092470811 1106 34-35 0.3437141042378187 1106 36-37 0.3314186451475294 1106 38-39 0.3889114149933022 1106 40-41 0.3859097350165186 1106 42-43 0.33269637817691233 1106 44-45 0.4208468273715127 1106 46-47 0.40682066660261995 1106 48-49 0.3399608839618864 1106 50-51 0.3060620349325305 1106 52-53 0.44353608974799386 1106 54-55 0.3465671932059706 1106 56-57 0.26230553081803265 1106 58-59 0.4905225256152832 1106 60-61 0.5431212553673319 1106 62-63 0.3695453233546644 1106 64-65 0.44207837281942375 1106 66-67 0.4563946000521426 1106 68-69 0.5180407517000134 1106 70-71 0.27030648879422614 1106 72-73 0.36341198904458594 1106 74-75 0.5137004879697749 1106 76-77 0.44872967714431766 1106 78-79 0.5969124560278374 1106 80-81 0.5218467139433365 1106 82-83 0.6196623215466524 1106 84-85 0.645254421181594 1106 86-87 0.5262136486259408 1106 88-89 0.6994223563338338 1106 90-91 0.835675860271575 1106 92-93 0.9205421523935478 1106 94-95 1.1018671134408748 1106 96-97 0.8414205066803149 1106 98-99 1.0195009356633271 1106 100-101 0.8921350754084862 1107 1 0.08502716083005879 1107 2 0.10602739817942108 1107 3 0.17499110340928326 1107 4 0.05871422029770912 1107 5 0.06853770386470615 1107 6 0.12823950011524232 1107 7 0.1343341824108748 1107 8 0.07504138949548889 1107 9 0.11581883457453301 1107 10-11 0.160476553729481 1107 12-13 0.06858066580218036 1107 14-15 0.21286547876849937 1107 16-17 0.2861543416898087 1107 18-19 0.19384723504848722 1107 20-21 0.2941322706384639 1107 22-23 0.319488462193533 1107 24-25 0.27784898695227866 1107 26-27 0.25079375825632866 1107 28-29 0.28826238251063074 1107 30-31 0.3241369567481982 1107 32-33 0.2600534833134205 1107 34-35 0.20970282118916828 1107 36-37 0.20025742614917164 1107 38-39 0.24276567302144514 1107 40-41 0.18145058334100383 1107 42-43 0.15041213527649688 1107 44-45 0.11072009421437912 1107 46-47 0.09693828140891014 1107 48-49 0.09647062162554931 1107 50-51 0.30473202711538505 1107 52-53 0.2761368758332665 1107 54-55 0.32819821917273373 1107 56-57 0.20591263650978675 1107 58-59 0.13771502380171086 1107 60-61 0.21571038029370015 1107 62-63 0.19937434931018316 1107 64-65 0.011604970161464223 1107 66-67 0.1679664957983391 1107 68-69 0.2528073282095633 1107 70-71 0.26250721965288193 1107 72-73 0.35872352110963135 1107 74-75 0.30497484868365277 1107 76-77 0.267394351944656 1107 78-79 0.35191803000881805 1107 80-81 0.17774021796266126 1107 82-83 0.3607840972213161 1107 84-85 0.4495798767763226 1107 86-87 0.5357430536518955 1107 88-89 0.7117072978442813 1107 90-91 0.7001312771977375 1107 92-93 0.654185324242011 1107 94-95 0.6600388145695426 1107 96-97 0.6901754081249791 1107 98-99 1.0632830911765723 1107 100-101 1.2956121667936316 1108 1 -0.023478534697694897 1108 2 0.04148524486110716 1108 3 0.07613950777972889 1108 4 0.0013696749379050743 1108 5 0.0820254831299394 1108 6 -0.010326397261046338 1108 7 -0.04097039405188241 1108 8 -0.0018980158609522846 1108 9 0.03366439398678267 1108 10-11 0.015020871428497173 1108 12-13 0.03512895954872164 1108 14-15 0.09204237280201966 1108 16-17 0.04190429960463149 1108 18-19 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-0.47533391448602913 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 10.0 4 16.0 5 47.0 6 99.0 7 225.0 8 481.0 9 999.0 10 1768.0 11 2746.0 12 4241.0 13 5792.0 14 7143.0 15 9871.0 16 12837.0 17 18157.0 18 16542.0 19 10552.0 20 9180.0 21 9619.0 22 10372.0 23 11425.0 24 13129.0 25 15332.0 26 17988.0 27 21829.0 28 26825.0 29 32466.0 30 39394.0 31 48438.0 32 60130.0 33 77021.0 34 105696.0 35 149831.0 36 235700.0 37 393658.0 38 334561.0 39 24163.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.162069943996755 21.044419027988855 15.420941186437826 21.372569841576563 2 14.795369279585993 22.707436972164295 40.020679471660905 22.4765142765888 3 19.239256973969557 26.91316936336852 31.051493851704926 22.796079810956996 4 12.133191072763504 17.99715787451599 39.392541966482355 30.47710908623814 5 12.126884238932952 39.58238345086803 34.708936725677034 13.581795584521988 6 28.795556748775088 38.599385286214535 18.04576099761382 14.559296967396563 7 26.259457357702786 34.67005422719877 22.05598154007096 17.014506875027486 8 24.874036905971472 34.98522233614383 20.85288066081732 19.28786009706738 9 25.754679207815386 17.888032290989212 22.17916731277961 34.178121188415794 10-11 23.958851670211608 27.59323699114266 27.982090906355666 20.465820432290062 12-13 25.718082213339937 25.6314355742459 28.107504322206307 20.542977890207858 14-15 22.357641253586706 26.94746788238092 27.851384371653058 22.84350649237932 16-17 20.3032051144816 30.135007918327528 28.44672906821797 21.115057898972903 18-19 20.11965636404928 29.05792311152835 31.044577492394907 19.777843032027462 20-21 21.661411933115122 27.460838300208934 30.445158576459992 20.432591190215955 22-23 21.03664675481576 27.628589977110245 30.426654415593735 20.908108852480265 24-25 21.202794216691238 27.765344121683704 30.780560370864972 20.251301290760086 26-27 20.868399446155518 27.495873071713373 31.35409536516878 20.281632116962324 28-29 20.209843983974856 28.64465562372851 31.011511996870883 20.133988395425753 30-31 20.741672086300632 27.82207669572825 30.62202161218874 20.814229605782383 32-33 20.511183347181365 28.097494393282584 30.626534759333534 20.764787500202512 34-35 21.366055780027576 27.64436109759393 30.620892890103473 20.368690232275018 36-37 21.171583659768206 27.358265927027304 30.958582915325987 20.5115674978785 38-39 20.677860183742638 28.168316473622617 30.786569515386386 20.367253827248362 40-41 20.763865321817462 28.07996187993046 30.673492172742705 20.482680625509374 42-43 20.49983813382037 28.230995044493675 29.818845852189995 21.450320969495966 44-45 20.885542560808908 27.768380092723184 29.838088831991666 21.507988514476246 46-47 21.4819687990733 28.252623125161286 29.4915991718944 20.77380890387101 48-49 21.2334412538257 28.213797718365413 29.457144783089067 21.095616244719817 50-51 20.504778587991257 28.45906268217146 29.87419591415177 21.161962815685513 52-53 21.06290493596967 28.852979764592884 28.897735179289263 21.186380120148183 54-55 20.57668184164177 28.231124051371186 29.261047374158416 21.931146732828633 56-57 21.211908177780785 28.26301608916871 28.819351324109682 21.705724408940824 58-59 21.593300502416028 28.002822509950825 28.91114069789712 21.49273628973603 60-61 21.079118333225324 28.53112820204227 29.48123906422137 20.90851440051103 62-63 20.676176413066997 28.357997803573763 29.552319034961133 21.4135067483981 64-65 20.421383291172052 28.677809896984524 29.278926380155113 21.62188043168831 66-67 20.71771976631088 28.377102308134276 28.533008848687047 22.3721690768678 68-69 20.46216937531191 28.297639970779905 29.03783424103688 22.202356412871307 70-71 21.067602315360208 28.128478884257447 28.75939371075587 22.044525089626475 72-73 20.836766411075956 27.740521812387314 29.205558808621728 22.217152967914995 74-75 20.30370604814142 29.012609624696882 28.43226485477205 22.25141947238965 76-77 20.571396748251402 28.02140141813691 28.730469871697075 22.676731961914616 78-79 20.5024122220795 28.240356608470147 28.767961478508713 22.48926969094164 80-81 20.863012347221968 28.336178641987846 28.377867404277136 22.422941606513053 82-83 20.76941636906897 28.01721244887993 28.803570478000147 22.409800704050955 84-85 20.30438863057229 28.16117027062682 28.593246248880394 22.9411948499205 86-87 20.302195407262342 28.377325178789732 28.432410922269153 22.88806849167878 88-89 20.380657716175673 28.099432518620077 28.133787488808498 23.386122276395753 90-91 20.27053803857311 27.7114655478285 28.42018747811445 23.597808935483936 92-93 20.727727618840422 28.211451358688734 28.222097757081592 22.838723265389252 94-95 20.294118327774832 27.650432452073847 28.450532435641364 23.604916784509953 96-97 19.750428748978756 27.69923808818458 28.864208660150766 23.6861245026859 98-99 20.01454622882942 28.06499282814215 27.894910729319218 24.025550213709213 100-101 19.844353473839522 27.344809130491388 28.665258711721226 24.145578683947868 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 811.0 1 544.0 2 353.5 3 422.0 4 390.0 5 402.5 6 701.0 7 1475.0 8 2337.5 9 2592.0 10 2256.5 11 2020.0 12 2111.0 13 2332.5 14 2539.0 15 2664.5 16 2861.0 17 3159.0 18 3342.0 19 3579.0 20 4033.5 21 4576.5 22 5241.0 23 6237.5 24 7627.5 25 9283.0 26 11590.5 27 14536.0 28 17011.5 29 19743.5 30 24113.0 31 28784.5 32 34115.0 33 39771.5 34 44350.0 35 48938.5 36 55007.5 37 59823.0 38 60576.5 39 60853.0 40 61186.0 41 60522.0 42 59221.0 43 60165.0 44 63042.5 45 64319.5 46 63444.5 47 69754.5 48 89172.5 49 86561.5 50 64056.5 51 51565.5 52 44927.0 53 42229.0 54 40548.0 55 36834.5 56 32041.5 57 28213.5 58 24608.5 59 20552.0 60 16764.5 61 13491.5 62 10696.5 63 7888.5 64 5607.0 65 4340.5 66 3446.5 67 2780.5 68 2142.0 69 1391.5 70 885.5 71 691.0 72 561.0 73 517.0 74 424.5 75 333.5 76 242.0 77 142.0 78 91.5 79 71.0 80 49.0 81 31.5 82 16.5 83 6.0 84 3.0 85 2.0 86 1.5 87 1.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2887835564062388 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0015622432424300635 16-17 8.679129124611464E-4 18-19 8.679129124611464E-5 20-21 5.78608608307431E-5 22-23 0.0 24-25 0.0 26-27 5.78608608307431E-5 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.001793686685753036 36-37 0.008360894390042378 38-39 0.0029798343327832695 40-41 0.0035005820802599574 42-43 2.6037387373834393E-4 44-45 0.0012440085078609766 46-47 1.157217216614862E-4 48-49 3.18234734569087E-4 50-51 8.679129124611464E-5 52-53 8.679129124611464E-5 54-55 0.0 56-57 5.496781778920593E-4 58-59 0.0020251301290760085 60-61 6.943303299689172E-4 62-63 0.001272938938276348 64-65 0.0 66-67 2.8930430415371544E-4 68-69 0.0012440085078609766 70-71 0.0 72-73 0.0 74-75 5.78608608307431E-5 76-77 0.2588116304959139 78-79 1.157217216614862E-4 80-81 2.893043041537155E-5 82-83 0.0 84-85 0.0 86-87 0.10903879223553538 88-89 0.3791911514542749 90-91 0.27943902738207377 92-93 0.0 94-95 0.0 96-97 0.0 98-99 5.78608608307431E-5 100-101 5.207477474766879E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1728284.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.08003024815847 #Duplication Level Percentage of deduplicated Percentage of total 1 66.2080963441385 25.874144078019228 2 15.221748889852837 11.89732814090643 3 6.741830554737703 7.904128260213253 4 3.539833590089024 5.533472150965058 5 2.124542929200332 4.1513600968330095 6 1.3594740197047614 3.18769714869886 7 0.9518849899254962 2.6039785939339493 8 0.6528651754266577 2.0411192642914457 9 0.47648959169412763 1.675910489070527 >10 2.421410748240415 17.138059488847407 >50 0.19423633610776053 5.256797399277494 >100 0.09988108036751238 6.702240309077842 >500 0.0032601252178133698 0.7855725318975844 >1k 0.003704687747515193 3.0178378333737568 >5k 5.927500396024309E-4 1.5152602504312784 >10k+ 1.4818750990060772E-4 0.7150939641630203 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 12348 0.7144659095380157 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8449 0.4888664131589484 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6354 0.3676479097185416 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 6262 0.36232471052211324 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5100 0.2950903902367898 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4665 0.26992091577541655 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4342 0.2512318577270865 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3272 0.1893207366381914 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3169 0.18336106797262486 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3063 0.1772278167245661 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2987 0.1728303913014296 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2801 0.16206827118691142 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2576 0.1490495774999942 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2409 0.1393868137412601 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1929 0.11161360054250344 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1872 0.10831553147515108 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 2.893043041537155E-5 0.0 18-19 0.0 0.0 0.0 8.679129124611464E-5 0.0 20-21 0.0 0.0 0.0 1.4465215207685775E-4 0.0 22-23 0.0 0.0 0.0 4.6288688664594473E-4 0.0 24-25 0.0 0.0 0.0 0.001272938938276348 0.0 26-27 0.0 0.0 0.0 0.002372295294060467 0.0 28-29 0.0 0.0 0.0 0.007695494490488831 0.0 30-31 0.0 0.0 0.0 0.023433648636450956 0.0 32-33 0.0 0.0 0.0 0.057687278248250866 0.0 34-35 0.0 0.0 0.0 0.10701366210645935 0.0 36-37 0.0 0.0 0.0 0.18203026817351778 0.0 38-39 0.0 0.0 0.0 0.30287267601852474 0.0 40-41 0.0 0.0 0.0 0.4267527790571457 0.0 42-43 0.0 0.0 0.0 0.5583862374470863 0.0 44-45 0.0 0.0 0.0 0.715883500628369 0.0 46-47 0.0 0.0 0.0 0.9083865846122512 0.0 48-49 0.0 0.0 0.0 1.1112756931152519 0.0 50-51 0.0 0.0 0.0 1.3273281474572467 0.0 52-53 0.0 0.0 0.0 1.544277445142118 0.0 54-55 0.0 0.0 0.0 1.7925583989668366 0.0 56-57 0.0 0.0 0.0 2.0896160584718713 0.0 58-59 0.0 0.0 0.0 2.3990848726251013 0.0 60-61 0.0 0.0 0.0 2.7431255511246992 0.0 62-63 0.0 0.0 0.0 3.087976281675928 0.0 64-65 0.0 0.0 0.0 3.4599926863871913 0.0 66-67 0.0 0.0 0.0 3.885067500480245 0.0 68-69 0.0 0.0 0.0 4.361551689421415 0.0 70-71 0.0 0.0 0.0 4.886205045004178 0.0 72-73 0.0 0.0 0.0 5.402092480171083 0.0 74-75 0.0 0.0 0.0 5.9416739378481775 0.0 76-77 0.0 0.0 0.0 6.529019536141051 0.0 78-79 0.0 0.0 0.0 7.217476988735648 0.0 80-81 0.0 0.0 0.0 7.9457427135817955 0.0 82-83 0.0 0.0 0.0 8.670826091082253 0.0 84-85 0.0 0.0 0.0 9.420355682283699 0.0 86-87 0.0 0.0 0.0 10.18935545315469 0.0 88-89 0.0 0.0 0.0 11.028164352617972 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGACGG 30 0.003967598 31.641258 32-33 GTATAGG 650 0.0 25.624622 1 GTACATG 10010 0.0 25.386915 1 TACATGG 10075 0.0 24.726994 2 ACATGGG 10075 0.0 23.785013 3 TACGGTG 180 1.971257E-7 23.72614 5 CATGGGG 3325 0.0 21.835184 4 GAGTACT 7840 0.0 21.458904 12-13 GTAAGCG 190 0.0 21.228653 94-95 AGTACTT 8375 0.0 20.739754 12-13 GTACTTT 8370 0.0 20.100094 14-15 TTACGGT 190 8.327213E-6 19.979908 4 TCGTATC 60 0.0047931876 19.771784 94-95 CATGGGT 1450 0.0 19.635426 4 CATGGGA 5850 0.0 19.629833 4 CGTATAG 180 1.2162801E-4 18.506672 1 TACTTTT 9675 0.0 17.879438 14-15 GTACACG 190 1.839959E-4 17.532637 1 ATGGGCG 245 3.8001526E-6 17.43145 5 TATAGGG 385 2.5465852E-10 17.255373 2 >>END_MODULE