FastQCFastQC Report
Fri 27 May 2016
SRR939292_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939292_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1759899
Sequences flagged as poor quality0
Sequence length101
%GC42

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT120950.6872553481762306No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87820.49900590886181534No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT63800.3625208037506698No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63730.36212305365251074No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT51050.29007346444313No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46310.2631401006535034No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC42760.24296848853258057No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT33450.19006772547742798No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT30770.17483957886219606No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC30250.1718848638473003No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC28950.16449807631006097No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG26560.15091775153005937No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC25980.14762210785959876No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA23000.1306893179665424No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA19490.11074499161599614No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA17700.1005739533916435No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG106600.026.602261
TACATGG107600.025.5644822
ACATGGG105950.024.7987673
GAGTACT77400.022.99355712-13
CATGGGG34550.022.2376174
AGTACTT82300.022.11406312-13
GTACTTT83500.021.001614-15
CATGGGA64500.020.9559064
CCTATAC4900.019.3577653
ATGGGAT29200.019.1655145
CATGGGT12900.019.1176684
AGAGTAC156550.018.83078210-11
CATGGGC17950.018.7592374
TACTTTT98300.018.39408514-15
CGACGCA650.007525658718.28623492-93
CCTTACG1051.9420531E-518.11208266-67
AGTACAT78600.018.1017282
ACTTTTT100150.018.0082616-17
CATTCCG1650.001486269117.2342419
GTATCAA252300.016.7749751
Produced by FastQC (version 0.11.5)