##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939292_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1759899 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.818349803028468 33.0 31.0 34.0 30.0 34.0 2 31.88280122893416 33.0 31.0 34.0 30.0 34.0 3 31.92824701872096 34.0 31.0 34.0 30.0 34.0 4 35.4436089798335 37.0 35.0 37.0 33.0 37.0 5 35.50330274635078 37.0 35.0 37.0 33.0 37.0 6 35.60747179241536 37.0 35.0 37.0 33.0 37.0 7 35.536055194076475 37.0 35.0 37.0 33.0 37.0 8 35.52308342694666 37.0 35.0 37.0 33.0 37.0 9 37.12926935011611 39.0 37.0 39.0 33.0 39.0 10-11 37.04143561647572 39.0 37.0 39.0 33.0 39.0 12-13 36.9461738997522 39.0 37.0 39.0 33.0 39.0 14-15 38.11671209541002 40.0 38.0 41.0 33.0 41.0 16-17 38.07829227699999 40.0 38.0 41.0 33.0 41.0 18-19 38.073706502475424 40.0 38.0 41.0 33.0 41.0 20-21 37.99252883262051 40.0 38.0 41.0 33.0 41.0 22-23 37.91099489232053 40.0 38.0 41.0 33.0 41.0 24-25 37.918545041505226 40.0 38.0 41.0 33.0 41.0 26-27 37.83031583062437 40.0 38.0 41.0 32.5 41.0 28-29 37.70091209779652 40.0 37.5 41.0 32.0 41.0 30-31 37.52299563781786 40.0 37.0 41.0 32.0 41.0 32-33 37.29354837976498 40.0 36.5 41.0 31.5 41.0 34-35 36.97078582350464 40.0 36.0 41.0 30.5 41.0 36-37 36.71140645002923 39.5 36.0 41.0 30.0 41.0 38-39 36.38161508132001 39.0 35.0 41.0 30.0 41.0 40-41 36.27675281365578 39.0 35.0 41.0 30.0 41.0 42-43 36.075191814984834 39.0 35.0 41.0 29.5 41.0 44-45 35.973404723793806 39.0 35.0 41.0 28.5 41.0 46-47 35.71921826195708 39.0 35.0 41.0 27.0 41.0 48-49 35.482198694356896 39.0 35.0 41.0 26.0 41.0 50-51 34.32987944194525 38.0 34.0 40.0 24.0 40.5 52-53 34.474737754837065 38.5 34.5 39.5 24.0 40.5 54-55 34.998103016139 39.0 35.0 40.5 24.5 41.0 56-57 34.90647815584872 39.0 35.0 41.0 24.0 41.0 58-59 34.730525160818885 39.0 35.0 41.0 23.5 41.0 60-61 34.4176859012932 38.0 34.0 40.0 23.0 41.0 62-63 34.08919489129774 38.0 34.0 40.0 22.0 41.0 64-65 33.733928765230274 37.5 34.0 40.0 21.5 41.0 66-67 33.36516328493851 37.0 33.0 40.0 19.5 41.0 68-69 32.94225435664206 36.0 33.0 39.0 18.5 41.0 70-71 32.51104637254752 36.0 32.5 39.0 16.0 40.5 72-73 32.05574410804256 35.0 32.0 38.5 12.0 40.0 74-75 31.598693163641776 35.0 31.5 37.5 8.0 39.5 76-77 31.137973826907114 35.0 31.0 37.0 6.5 39.0 78-79 30.682989478373475 35.0 31.0 36.5 2.0 39.0 80-81 30.182971579619057 35.0 30.5 36.0 2.0 38.5 82-83 29.70775112662715 34.0 30.0 35.5 2.0 37.0 84-85 29.255968666383694 34.0 29.0 35.0 2.0 37.0 86-87 28.933933992802995 34.0 29.0 35.0 2.0 36.0 88-89 28.479884925214456 34.0 29.0 35.0 2.0 36.0 90-91 28.04252687228074 34.0 28.5 35.0 2.0 36.0 92-93 27.596928289634803 34.0 27.0 35.0 2.0 35.0 94-95 27.09861588647985 34.0 26.0 35.0 2.0 35.0 96-97 26.50634951210268 33.5 25.0 35.0 2.0 35.0 98-99 25.77476775655876 33.0 23.0 35.0 2.0 35.0 100-101 23.381675027941945 31.0 9.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.33558741113705537 1101 2 0.24580539508653843 1101 3 0.3209938235906584 1101 4 0.45709068079290205 1101 5 0.4325479286607816 1101 6 0.32640270989845277 1101 7 0.33819351711665746 1101 8 0.3756656517395669 1101 9 0.42437137526165003 1101 10-11 0.45309393650239116 1101 12-13 0.43257745577110995 1101 14-15 0.5116740831535083 1101 16-17 0.581070090671922 1101 18-19 0.6573522528187752 1101 20-21 0.6909526752955415 1101 22-23 0.6343409785023724 1101 24-25 0.7823174323410669 1101 26-27 0.8120790586951259 1101 28-29 0.6564927559078271 1101 30-31 0.7322996813633225 1101 32-33 0.7871491669101189 1101 34-35 0.824809690710822 1101 36-37 0.8031748587761811 1101 38-39 0.9474853930039089 1101 40-41 0.9842890216949769 1101 42-43 0.9082219353152325 1101 44-45 1.023680860056075 1101 46-47 0.8945138319279877 1101 48-49 0.9885661666533494 1101 50-51 0.8856138029591989 1101 52-53 0.97683229749547 1101 54-55 1.0597628581556862 1101 56-57 1.06750537179515 1101 58-59 0.9419278838638476 1101 60-61 1.060442857362176 1101 62-63 1.175566210134896 1101 64-65 1.0733231484561259 1101 66-67 0.9835102146688399 1101 68-69 1.001488664401883 1101 70-71 0.9659945502244867 1101 72-73 1.092234603417296 1101 74-75 1.0075197189207152 1101 76-77 0.9770303937113809 1101 78-79 0.8057186181300757 1101 80-81 0.7797335717166156 1101 82-83 0.8193123293453723 1101 84-85 0.5804977663015833 1101 86-87 0.74188750693164 1101 88-89 0.5037772865724115 1101 90-91 0.5536493617107787 1101 92-93 0.5696505004910186 1101 94-95 0.4964181260787015 1101 96-97 0.43281492503062324 1101 98-99 0.5328825799444843 1101 100-101 0.5539105522066805 1102 1 0.3126413280275955 1102 2 0.440042345815133 1102 3 0.4581432353198487 1102 4 0.4126838006444231 1102 5 0.3241282374361987 1102 6 0.42784681972138827 1102 7 0.4328834065677256 1102 8 0.3930850620059161 1102 9 0.447773338254116 1102 10-11 0.39451646749487423 1102 12-13 0.581011442583879 1102 14-15 0.7399447117182163 1102 16-17 0.7290956044655772 1102 18-19 0.5848545805925411 1102 20-21 0.6633442722450908 1102 22-23 0.6494314540183765 1102 24-25 0.6386976609311645 1102 26-27 0.7233070938373416 1102 28-29 0.7805543902488168 1102 30-31 0.7876126556738399 1102 32-33 0.7929811786561842 1102 34-35 0.70258183126424 1102 36-37 0.7429801384689441 1102 38-39 0.8050126742028496 1102 40-41 0.9410872433764368 1102 42-43 0.832769944730039 1102 44-45 0.791487071632325 1102 46-47 0.8742175022641447 1102 48-49 0.8686637590038586 1102 50-51 0.7813997778029886 1102 52-53 0.9082212141189885 1102 54-55 0.8095807770988515 1102 56-57 0.841452029978889 1102 58-59 0.7324021227805062 1102 60-61 0.7141597102894437 1102 62-63 0.8150979618978482 1102 64-65 0.8443977246774637 1102 66-67 0.9173706736292004 1102 68-69 0.7559265273546387 1102 70-71 0.8815033284276979 1102 72-73 0.8667989053134946 1102 74-75 0.8604578221972439 1102 76-77 0.8221231447568478 1102 78-79 0.7605979260595213 1102 80-81 0.7167691295735032 1102 82-83 0.5642545007113178 1102 84-85 0.4045304534309899 1102 86-87 0.28256569211175275 1102 88-89 0.3346891933964464 1102 90-91 0.4141863015261187 1102 92-93 0.5718699157109057 1102 94-95 0.7958238181531279 1102 96-97 0.7925045397153703 1102 98-99 0.6991560598869349 1102 100-101 0.39130578303747043 1103 1 0.3789039595900974 1103 2 0.3886349294343141 1103 3 0.3547334995115854 1103 4 0.46723355510223996 1103 5 0.34744789674424936 1103 6 0.2901001442533442 1103 7 0.4632948348790151 1103 8 0.4642558366028666 1103 9 0.3552720872987791 1103 10-11 0.3922022483475516 1103 12-13 0.46193013955330287 1103 14-15 0.6634626334618403 1103 16-17 0.6048766076140879 1103 18-19 0.7522752283578527 1103 20-21 0.7869523553648534 1103 22-23 0.7906435747628606 1103 24-25 0.6861600830816244 1103 26-27 0.6910698687244121 1103 28-29 0.6595994512611014 1103 30-31 0.5538761359955444 1103 32-33 0.6799754483741154 1103 34-35 0.6533094300974227 1103 36-37 0.6952430269740049 1103 38-39 0.8478034484988726 1103 40-41 0.7690483810069892 1103 42-43 0.6033563100306054 1103 44-45 0.8358494007664845 1103 46-47 0.671986731579203 1103 48-49 0.7411826332867975 1103 50-51 0.9104977898160129 1103 52-53 0.8008799870677947 1103 54-55 0.9532967375142647 1103 56-57 1.073969808590853 1103 58-59 1.0549726749253807 1103 60-61 1.0008142448433972 1103 62-63 0.8926778330330549 1103 64-65 0.9107369374687266 1103 66-67 0.9180589401889776 1103 68-69 0.9996701110243578 1103 70-71 0.8998498374617725 1103 72-73 0.8967489172539942 1103 74-75 0.8893665609063355 1103 76-77 0.786624755277451 1103 78-79 0.7886706369462892 1103 80-81 0.6698618731066297 1103 82-83 0.7793179319593122 1103 84-85 0.8211790196644912 1103 86-87 0.8076627303664878 1103 88-89 0.8313798040748424 1103 90-91 0.8321166870672734 1103 92-93 0.8899210179597716 1103 94-95 0.8897405679917654 1103 96-97 0.8803459097053299 1103 98-99 0.6901962490213478 1103 100-101 0.5984609003417809 1104 1 0.16392482462271118 1104 2 0.24583788518839 1104 3 0.309925277031585 1104 4 0.3029387480096304 1104 5 0.25337710628340915 1104 6 0.20577829547587356 1104 7 0.19417094027379278 1104 8 0.3081187299878181 1104 9 0.42685120117515396 1104 10-11 0.3659384826987804 1104 12-13 0.39307402542082315 1104 14-15 0.37513594128510164 1104 16-17 0.5027300326430861 1104 18-19 0.3339605128604788 1104 20-21 0.3463807890562336 1104 22-23 0.47896311106199363 1104 24-25 0.5270555470084659 1104 26-27 0.4107057852040228 1104 28-29 0.4478065649567 1104 30-31 0.4956459351790201 1104 32-33 0.5138722756839584 1104 34-35 0.6594887187278715 1104 36-37 0.6908233309858147 1104 38-39 0.842118103736226 1104 40-41 0.7161988352169075 1104 42-43 0.7006592974211969 1104 44-45 0.7364762933078026 1104 46-47 0.7445070316741109 1104 48-49 0.6546805015885226 1104 50-51 0.770487771587355 1104 52-53 0.7470717419902826 1104 54-55 0.7802403115361685 1104 56-57 0.7402631769754322 1104 58-59 0.7488800100765545 1104 60-61 0.7743444652444254 1104 62-63 0.7037487289489306 1104 64-65 0.8541275429312947 1104 66-67 0.830631440527938 1104 68-69 0.8312386417343731 1104 70-71 0.7830926552204076 1104 72-73 0.8025060091053291 1104 74-75 0.7333686172795915 1104 76-77 0.6749041809994409 1104 78-79 0.5450335303312421 1104 80-81 0.5258385374131151 1104 82-83 0.4191264557394234 1104 84-85 0.40607541008917636 1104 86-87 0.49783168440315606 1104 88-89 0.5076919660537769 1104 90-91 0.6027717511688699 1104 92-93 0.6058660080921925 1104 94-95 0.5456882321626644 1104 96-97 0.4652989822132021 1104 98-99 0.38842667581295487 1104 100-101 0.1622401076211908 1105 1 0.19357136172979494 1105 2 0.2259159252736076 1105 3 0.31446892006159644 1105 4 0.4040984801951879 1105 5 0.2994986517234679 1105 6 0.21536338597918103 1105 7 0.2944850570513253 1105 8 0.23894528825340444 1105 9 0.35566661499773744 1105 10-11 0.4042797504697475 1105 12-13 0.2594957751998166 1105 14-15 0.3340687050779749 1105 16-17 0.4484438059572966 1105 18-19 0.3631087020696455 1105 20-21 0.3030406444248328 1105 22-23 0.42806876588858955 1105 24-25 0.25868895425162464 1105 26-27 0.3738072083305539 1105 28-29 0.363747900840643 1105 30-31 0.4415245563983916 1105 32-33 0.2980840128548081 1105 34-35 0.4359843058762536 1105 36-37 0.4559766576527693 1105 38-39 0.5140249411201054 1105 40-41 0.3925781230739531 1105 42-43 0.352922604813628 1105 44-45 0.39898209015721164 1105 46-47 0.42265109787413024 1105 48-49 0.23681787242747276 1105 50-51 0.07917471273484011 1105 52-53 0.0275383521376682 1105 54-55 0.048718674795729555 1105 56-57 0.1174339159288138 1105 58-59 0.012616731668373404 1105 60-61 0.07705938343639218 1105 62-63 0.17755747639468922 1105 64-65 0.07163946270290467 1105 66-67 0.006905660489387344 1105 68-69 0.05601600648662952 1105 70-71 -0.05106926527579958 1105 72-73 -0.004426298185656208 1105 74-75 0.03609875220125858 1105 76-77 0.09938282685541822 1105 78-79 -0.023686851391293118 1105 80-81 0.0299561120113232 1105 82-83 -0.08945940284643683 1105 84-85 0.10197649410001475 1105 86-87 0.055947635883526914 1105 88-89 0.10149622094124666 1105 90-91 0.10556420222172491 1105 92-93 0.17270929751438757 1105 94-95 0.1275462673205574 1105 96-97 0.43681181498858734 1105 98-99 0.6021240875196909 1105 100-101 0.3443162492382221 1106 1 0.201066708265067 1106 2 0.29074901270479003 1106 3 0.23274605558860983 1106 4 0.2561380154505031 1106 5 0.2705219712710587 1106 6 0.2691872508692654 1106 7 0.3266754122917348 1106 8 0.2597705372509438 1106 9 0.29065057083357715 1106 10-11 0.34148754712423113 1106 12-13 0.3565340895907312 1106 14-15 0.3904923540168639 1106 16-17 0.4431709168249256 1106 18-19 0.5593023028034096 1106 20-21 0.3941589769661604 1106 22-23 0.4709074450862403 1106 24-25 0.49068881314321544 1106 26-27 0.5088144055509929 1106 28-29 0.4821729580967613 1106 30-31 0.4983365993091766 1106 32-33 0.5181174984330497 1106 34-35 0.5052488198096725 1106 36-37 0.5726591298443822 1106 38-39 0.6938710637861973 1106 40-41 0.8293579254806858 1106 42-43 0.5976218744392838 1106 44-45 0.681968428234967 1106 46-47 0.6104366435313509 1106 48-49 0.6091773640775173 1106 50-51 0.7143525888734814 1106 52-53 0.740959629210252 1106 54-55 0.6693857302022295 1106 56-57 0.6878727769072839 1106 58-59 0.8188812877959677 1106 60-61 0.765445504456217 1106 62-63 0.669674081741455 1106 64-65 0.7561385313182427 1106 66-67 0.6442292144567787 1106 68-69 0.7000683217366444 1106 70-71 0.7137614703486008 1106 72-73 0.6588122642517007 1106 74-75 0.7767681317462056 1106 76-77 0.7299710483153774 1106 78-79 0.6508326018551394 1106 80-81 0.6091564907905536 1106 82-83 0.7193592846102064 1106 84-85 0.7101772443263776 1106 86-87 0.8070759843548885 1106 88-89 0.8586552245222201 1106 90-91 0.7106591840886018 1106 92-93 0.5685292722393847 1106 94-95 0.41301410740046407 1106 96-97 0.44509397923185645 1106 98-99 0.6247738889649455 1106 100-101 0.6578930222411401 1107 1 0.06188195190196666 1107 2 0.1862553627927106 1107 3 0.15965300902876933 1107 4 0.13161936970549704 1107 5 0.25927417586531476 1107 6 0.06489737563101983 1107 7 0.18850162339386856 1107 8 0.1295319700115627 1107 9 0.13242148013770816 1107 10-11 0.09963639289519932 1107 12-13 0.16912134277005464 1107 14-15 0.39674328872529685 1107 16-17 0.27105774117484316 1107 18-19 0.3413460843817333 1107 20-21 0.40320991931697137 1107 22-23 0.44806448862075854 1107 24-25 0.32585466912532013 1107 26-27 0.2540911220709745 1107 28-29 0.3634593870197236 1107 30-31 0.29180605913170865 1107 32-33 0.20806820512203217 1107 34-35 0.3962511105453359 1107 36-37 0.5358324410938948 1107 38-39 0.6746030582546965 1107 40-41 0.48223883687328595 1107 42-43 0.5316685179548273 1107 44-45 0.5966670189417158 1107 46-47 0.5308442851145614 1107 48-49 0.590475289502443 1107 50-51 0.5677799299928097 1107 52-53 0.5074356977096386 1107 54-55 0.6377024907844415 1107 56-57 0.6863266785696993 1107 58-59 0.7229110258536124 1107 60-61 0.5187422663956198 1107 62-63 0.5939101573034478 1107 64-65 0.6701830731809935 1107 66-67 0.5957638821749285 1107 68-69 0.6589342329941914 1107 70-71 0.5584101286837608 1107 72-73 0.48734803154932393 1107 74-75 0.541943679048881 1107 76-77 0.5800513920640817 1107 78-79 0.5662840652050143 1107 80-81 0.5222928419391835 1107 82-83 0.5866137222929027 1107 84-85 0.5464209385444576 1107 86-87 0.7614668245745904 1107 88-89 0.780283998850166 1107 90-91 0.8732651808484988 1107 92-93 0.7886373788873939 1107 94-95 0.9094030857797932 1107 96-97 1.0381411979019113 1107 98-99 0.9786321018485395 1107 100-101 1.0677116937462188 1108 1 0.11359847628880715 1108 2 0.17795227886754006 1108 3 0.1529431403088104 1108 4 0.23600126947992095 1108 5 0.21349562225730523 1108 6 0.12881505260400417 1108 7 0.22684527898901052 1108 8 0.17384356681365176 1108 9 0.16833179203739945 1108 10-11 0.1481962978825635 1108 12-13 0.22345533768464776 1108 14-15 0.23207427862622154 1108 16-17 0.2989160511934159 1108 18-19 0.2686229999783123 1108 20-21 0.3849448563870439 1108 22-23 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2316 52-53 -2.0186761425293795 2316 54-55 -1.8534393798525812 2316 56-57 -2.1070551405954987 2316 58-59 -1.5854281034127453 2316 60-61 -1.6035985412264537 2316 62-63 -1.6197082148041844 2316 64-65 -1.8519824620463048 2316 66-67 -1.8210111677348806 2316 68-69 -1.71309311535871 2316 70-71 -1.7445454732016934 2316 72-73 -2.033606484672667 2316 74-75 -1.8144847923769625 2316 76-77 -1.6743242908771165 2316 78-79 -1.853043871361141 2316 80-81 -1.6770609150507987 2316 82-83 -1.575572399412394 2316 84-85 -1.5731244405828022 2316 86-87 -1.468059306139402 2316 88-89 -1.571787874662995 2316 90-91 -1.545280762519532 2316 92-93 -1.7225747584583324 2316 94-95 -1.8105337688328547 2316 96-97 -1.9446562193796346 2316 98-99 -1.9392052306081489 2316 100-101 -1.5010749745092227 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5091.0 3 1286.0 4 1255.0 5 1416.0 6 1643.0 7 2071.0 8 2642.0 9 3228.0 10 4008.0 11 5506.0 12 7500.0 13 10908.0 14 10571.0 15 11873.0 16 15214.0 17 20097.0 18 16169.0 19 11201.0 20 10910.0 21 11341.0 22 12244.0 23 13849.0 24 15576.0 25 17434.0 26 20556.0 27 23916.0 28 27902.0 29 33198.0 30 39772.0 31 48035.0 32 59564.0 33 75573.0 34 102629.0 35 143937.0 36 216375.0 37 342068.0 38 351955.0 39 61367.0 40 19.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.975731698769685 20.435882372047093 15.000544183420015 21.587841745763203 2 14.532053750663406 22.219390671849162 40.58825838225816 22.66029719522927 3 19.276636173311488 26.81139632259239 30.782416257054436 23.12955124704169 4 11.892992198509337 17.374004475062023 39.70004682146004 31.032956504968595 5 12.19371621106485 39.45449538632751 34.68128734491654 13.670501057691103 6 28.900459324039208 38.193257220893415 18.33060528549556 14.575678169571816 7 26.345194780531102 34.52510713739457 22.258365871146278 16.871332210928042 8 24.89992266751581 34.49466633307556 21.139175726697903 19.46623527271073 9 26.10143875197094 17.678175128235598 22.279304272473002 33.94108184732046 10-11 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30.553242412574587 20.417897916918232 36-37 21.15385876593057 27.304046318592878 30.912385604281706 20.629709311194848 38-39 20.67956795038117 28.167802776663663 30.727193065723913 20.42543620723125 40-41 20.758965852379447 28.081850543950704 30.667476108636233 20.491707495033616 42-43 20.42735754225938 28.2761103866818 29.750876774859726 21.545655296199094 44-45 20.83007820296275 27.8051620570219 29.817659476677683 21.547100263337672 46-47 21.42783821745624 28.269573702096164 29.49327046618296 20.80931761426464 48-49 21.230396762819186 28.198206033783368 29.40886945338354 21.1625277500139 50-51 20.485811211365746 28.52220399497114 29.85409866496871 21.137886128694404 52-53 20.999668368358066 28.8899350794704 28.892586991998353 21.21780956017318 54-55 20.484338087246886 28.223289671685166 29.371966792742025 21.920405448325923 56-57 21.157757285651943 28.300930012875437 28.898781180600707 21.642531520871916 58-59 21.60967846559076 27.944833726542136 29.05024541587317 21.39524239199393 60-61 21.065504979919083 28.391008398717144 29.676397844285585 20.867088777078184 62-63 20.74725052925423 28.209806469416716 29.78881715599045 21.2541258453386 64-65 20.485018168005716 28.5612114361194 29.511645375128964 21.442125020745916 66-67 20.73833044351159 28.183176674575655 28.770333318123747 22.30815956378901 68-69 20.464847514602244 28.152306568564452 29.325762612377886 22.057083304455414 70-71 21.05296483091418 27.92209847982912 29.145074653769594 21.8798620354871 72-73 20.795066133364053 27.461415312735443 29.59512955477825 22.14838899912225 74-75 20.31720456172271 28.716979840699032 28.930493331089924 22.035322266488336 76-77 20.569868482018748 27.742951848023665 29.143899918024026 22.543279751933564 78-79 20.48631709359144 27.905078711953564 29.2693926338622 22.339211560592794 80-81 20.934625848633633 27.93521193890978 28.900122919322506 22.230039293134077 82-83 20.862766631684558 27.526644993153255 29.365016235177865 22.245572139984322 84-85 20.369393546091363 27.658610495870217 29.26875166799346 22.703244290044957 86-87 20.41707199414276 27.84998910890299 29.164903753493988 22.568035143460268 88-89 20.5259952197288 27.44144852140657 28.923891489782754 23.108664769081876 90-91 20.43602776526786 27.026243066740406 29.300703030455143 23.23702613753659 92-93 20.965930448581382 27.436971985838056 29.152627643172373 22.444469922408192 94-95 20.558994608268844 26.809752174303647 29.350781950748583 23.28047126667893 96-97 20.00555029966199 26.724749915647422 29.913919016393347 23.355780768297237 98-99 20.347806396765314 26.969839648811305 29.06284373751423 23.619510216909156 100-101 20.215204663689608 26.087100322587453 29.796884524869228 23.90081048885371 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1974.0 1 1157.5 2 432.5 3 532.5 4 482.0 5 506.5 6 846.0 7 1648.5 8 2596.0 9 2945.0 10 2688.5 11 2540.5 12 2710.5 13 2872.5 14 3057.5 15 3279.5 16 3459.0 17 3644.5 18 3862.0 19 4141.5 20 4475.5 21 4958.0 22 5654.5 23 6678.5 24 7871.0 25 9381.5 26 11888.0 27 14980.5 28 17239.5 29 19948.5 30 24645.0 31 29454.0 32 34606.0 33 40102.5 34 44994.0 35 49543.5 36 55558.0 37 60944.0 38 61858.0 39 61804.0 40 61610.0 41 61226.0 42 60232.0 43 60911.0 44 63450.0 45 64658.0 46 64448.0 47 69784.0 48 88482.5 49 86940.5 50 64230.0 51 51376.0 52 45179.0 53 42692.5 54 40800.5 55 36985.0 56 32538.0 57 28966.0 58 25210.0 59 21035.5 60 17580.0 61 14458.5 62 11448.0 63 8419.0 64 5974.0 65 4656.0 66 3765.5 67 3072.0 68 2379.5 69 1570.0 70 1056.0 71 814.5 72 643.5 73 574.5 74 493.5 75 387.5 76 266.5 77 173.0 78 121.0 79 94.5 80 63.5 81 40.5 82 27.5 83 21.0 84 19.5 85 13.0 86 7.0 87 5.5 88 4.0 89 3.5 90 2.5 91 2.5 92 2.5 93 2.0 94 2.5 95 2.0 96 1.0 97 2.0 98 1.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2827434983484848 2 0.10966538420670731 3 0.12154106570888444 4 0.12262067311817326 5 0.13051885363875995 6 0.05738965702009036 7 0.06682201649071907 8 0.07153819622603343 9 0.06943580285004992 10-11 0.06395253363971455 12-13 0.06827096327686986 14-15 0.0642366408526853 16-17 0.0651173732128946 18-19 0.06980514222691189 20-21 0.07378264320850231 22-23 0.08307294907264565 24-25 0.09071543310155866 26-27 0.09136887969139139 28-29 0.08537421749770867 30-31 0.0815671808439007 32-33 0.0738962860936906 34-35 0.2404115236158439 36-37 0.3631742503405025 38-39 0.3345362432730515 40-41 0.31842736429761026 42-43 0.34251965595752937 44-45 0.3555317663115895 46-47 0.36138437489878683 48-49 0.37056103787774186 50-51 0.36476525073313865 52-53 0.36635625112577486 54-55 0.35149744388740495 56-57 0.32862681324326 58-59 0.31422257754564326 60-61 0.26990185232220715 62-63 0.34183779864639957 64-65 0.3717258774509219 66-67 0.37610112853067135 68-69 0.3880620421967397 70-71 0.3886302566226812 72-73 0.3720668061064868 74-75 0.3758170213177006 76-77 0.3600490709978243 78-79 0.33754777973054134 80-81 0.3354169756332608 82-83 0.3491961754623419 84-85 0.3576909811301671 86-87 0.3507587651336809 88-89 0.366043733191507 90-91 0.3889711852782461 92-93 0.3835447375105049 94-95 0.38362996967439605 96-97 0.38868707806527536 98-99 0.3780614683001695 100-101 0.3560431592949368 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1759899.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.31467869490085 #Duplication Level Percentage of deduplicated Percentage of total 1 67.49706927609928 27.21122660713406 2 14.818956020091484 11.948429010877096 3 6.389970042671657 7.728287674210491 4 3.3678006120349955 5.4308719833072505 5 2.0276570121202866 4.087217047354601 6 1.3073959162236621 3.1624347773749464 7 0.8881674579344387 2.506432998572563 8 0.6470089989940043 2.0867167925722168 9 0.4697706182463547 1.704478638141604 >10 2.301279743963239 16.678389794824753 >50 0.18469945837672364 5.165040791169218 >100 0.09272240153424109 6.3486475886318265 >500 0.0032557765910014662 0.8006020140818877 >1k 0.0035388875989146376 2.930708990888541 >5k 5.66222015826342E-4 1.5202821052920015 >10k+ 1.415555039565855E-4 0.6902331855670705 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 12095 0.6872553481762306 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8782 0.49900590886181534 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 6380 0.3625208037506698 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6373 0.36212305365251074 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5105 0.29007346444313 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4631 0.2631401006535034 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4276 0.24296848853258057 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3345 0.19006772547742798 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3077 0.17483957886219606 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3025 0.1718848638473003 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2895 0.16449807631006097 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2656 0.15091775153005937 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2598 0.14762210785959876 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2300 0.1306893179665424 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1949 0.11074499161599614 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1770 0.1005739533916435 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.8410721297074434E-5 0.0 12-13 0.0 0.0 0.0 1.1364288518829774E-4 0.0 14-15 0.0 0.0 0.0 1.704643277824466E-4 0.0 16-17 0.0 0.0 0.0 3.125179342678188E-4 0.0 18-19 0.0 0.0 0.0 4.5457154075319095E-4 0.0 20-21 0.0 0.0 0.0 5.113929833473398E-4 0.0 22-23 0.0 0.0 0.0 8.807323602093074E-4 0.0 24-25 0.0 0.0 0.0 0.0015910003926361683 0.0 26-27 0.0 0.0 0.0 0.0030399471787869647 0.0 28-29 0.0 0.0 0.0 0.00786976979928962 0.0 30-31 0.0 0.0 0.0 0.022842219922847844 0.0 32-33 0.0 0.0 0.0 0.05474745993946244 0.0 34-35 0.0 0.0 0.0 0.09929547093327515 0.0 36-37 0.0 0.0 0.0 0.17199850673248862 0.0 38-39 0.0 0.0 0.0 0.2793626225141329 0.0 40-41 0.0 0.0 0.0 0.39848877691276596 0.0 42-43 0.0 0.0 0.0 0.5246039687504794 0.0 44-45 0.0 0.0 0.0 0.6793287569343468 0.0 46-47 0.0 0.0 0.0 0.8705613219849548 0.0 48-49 0.0 0.0 0.0 1.0649474770995382 0.0 50-51 0.0 0.0 0.0 1.2762380113858807 0.0 52-53 0.0 0.0 0.0 1.483948794788792 0.0 54-55 0.0 0.0 0.0 1.7184792990961415 0.0 56-57 0.0 0.0 0.0 2.0039502266891454 0.0 58-59 0.0 0.0 0.0 2.3159567679736166 0.0 60-61 0.0 0.0 0.0 2.6512601007216894 0.0 62-63 0.0 0.0 0.0 2.9942343282199717 0.0 64-65 0.0 0.0 0.0 3.3555902923974616 0.0 66-67 0.0 0.0 0.0 3.775983735430272 0.0 68-69 0.0 0.0 0.0 4.2473744231913315 0.0 70-71 0.0 0.0 0.0 4.752119297755155 0.0 72-73 0.0 0.0 0.0 5.256949404482871 0.0 74-75 0.0 0.0 0.0 5.794508662144816 0.0 76-77 0.0 0.0 0.0 6.370109875623545 0.0 78-79 0.0 0.0 0.0 7.044665631379983 0.0 80-81 0.0 0.0 0.0 7.7734574540925365 0.0 82-83 0.0 0.0 0.0 8.494351096284504 0.0 84-85 0.0 0.0 0.0 9.23456402895848 0.0 86-87 0.0 0.0 0.0 10.011739310039951 0.0 88-89 0.0 0.0 0.0 10.847781605648962 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 10660 0.0 26.60226 1 TACATGG 10760 0.0 25.564482 2 ACATGGG 10595 0.0 24.798767 3 GAGTACT 7740 0.0 22.993557 12-13 CATGGGG 3455 0.0 22.237617 4 AGTACTT 8230 0.0 22.114063 12-13 GTACTTT 8350 0.0 21.0016 14-15 CATGGGA 6450 0.0 20.955906 4 CCTATAC 490 0.0 19.357765 3 ATGGGAT 2920 0.0 19.165514 5 CATGGGT 1290 0.0 19.117668 4 AGAGTAC 15655 0.0 18.830782 10-11 CATGGGC 1795 0.0 18.759237 4 TACTTTT 9830 0.0 18.394085 14-15 CGACGCA 65 0.0075256587 18.286234 92-93 CCTTACG 105 1.9420531E-5 18.112082 66-67 AGTACAT 7860 0.0 18.101728 2 ACTTTTT 10015 0.0 18.00826 16-17 CATTCCG 165 0.0014862691 17.234241 9 GTATCAA 25230 0.0 16.774975 1 >>END_MODULE