FastQCFastQC Report
Fri 27 May 2016
SRR939292_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939292_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1759899
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT121370.6896418487651849No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87370.49644894394507866No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT64640.3672938049285783No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63600.36138437489878683No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT51700.29376685821174964No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47070.2674585302906587No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC42920.24387763161408693No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT32680.1856924743976785No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT31670.17995350869566948No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC31420.17853297263081574No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC30640.17410090010847212No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG28650.1627934330322365No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC25410.14438328563173228No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA24410.13870114137231737No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA20020.11375652807348603No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA19580.11125638459934348No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATCG550.002591450234.5142635
CCGTGCG702.064355E-433.8989039
GCGTCCG851.6424983E-533.500099
GTACATG104200.025.9781571
TACATGG103800.025.6030672
CATGGGG35650.025.1595924
ACATGGG104150.024.5601373
GAGTACT74450.021.86529712-13
GTATAGG6100.021.8369481
AGTACTT79450.020.99700712-13
CATGGGA58000.020.6192994
GTACTTT78650.020.54680814-15
ATGGGGG18200.018.5134895
ATGGGGA22150.018.4257875
GTACACG2403.0467872E-617.8400061
ATACCGT1600.00119859517.796426
CCATGCG1350.00944980917.5772089
TACTTTT94900.017.50361814-15
AGAGTAC152850.017.41875810-11
GTCCCGA1650.00147311617.2571336
Produced by FastQC (version 0.11.5)