##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939292_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1759899 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.23012172857647 33.0 31.0 34.0 31.0 34.0 2 32.42354760131121 34.0 31.0 34.0 31.0 34.0 3 32.51165379376884 34.0 31.0 34.0 30.0 34.0 4 36.05967501544123 37.0 35.0 37.0 35.0 37.0 5 36.00196318084163 37.0 35.0 37.0 35.0 37.0 6 36.04808685043858 37.0 35.0 37.0 35.0 37.0 7 35.99404454460171 37.0 35.0 37.0 35.0 37.0 8 35.977674286990336 37.0 35.0 37.0 35.0 37.0 9 37.66485747193447 39.0 37.0 39.0 35.0 39.0 10-11 37.58094555426192 39.0 37.0 39.0 35.0 39.0 12-13 37.51508097907892 39.0 37.0 39.0 35.0 39.0 14-15 38.75658517903584 40.0 38.0 41.0 34.0 41.0 16-17 38.72634935300265 40.0 38.0 41.0 34.0 41.0 18-19 38.873771449384314 40.0 38.0 41.0 35.0 41.0 20-21 38.8889745945648 40.0 38.0 41.0 35.0 41.0 22-23 38.82797166201015 40.0 38.0 41.0 34.5 41.0 24-25 38.75509333206053 40.0 38.0 41.0 34.0 41.0 26-27 38.58057564667064 40.0 38.0 41.0 34.0 41.0 28-29 38.440750577163804 40.0 38.0 41.0 34.0 41.0 30-31 38.25265853324537 40.0 38.0 41.0 34.0 41.0 32-33 38.02428917795851 40.0 37.0 41.0 33.0 41.0 34-35 37.93678728154286 40.0 37.0 41.0 33.0 41.0 36-37 37.83435413054954 40.0 37.0 41.0 33.0 41.0 38-39 37.716357302322464 40.0 37.0 41.0 33.0 41.0 40-41 37.55889485703441 40.0 37.0 41.0 33.0 41.0 42-43 37.35964762750589 40.0 37.0 41.0 32.0 41.0 44-45 37.096535653466475 40.0 36.5 41.0 31.5 41.0 46-47 36.76208606289338 40.0 36.0 41.0 31.0 41.0 48-49 36.416082400183186 40.0 36.0 41.0 30.0 41.0 50-51 36.186016072513254 39.0 36.0 41.0 30.0 41.0 52-53 35.994282910553395 39.0 35.0 41.0 29.0 41.0 54-55 35.749906102566115 39.0 35.0 40.5 28.0 41.0 56-57 35.51398290470078 39.0 35.0 40.0 28.0 41.0 58-59 35.24430151957584 38.5 35.0 40.0 27.5 41.0 60-61 34.89907261723542 38.0 34.0 40.0 26.5 41.0 62-63 34.563199649525345 38.0 34.0 40.0 26.0 41.0 64-65 34.16793406894373 37.0 34.0 40.0 26.0 41.0 66-67 33.7900464742579 37.0 33.5 39.0 25.0 41.0 68-69 33.411917956655465 36.0 33.0 39.0 24.0 40.0 70-71 33.03151601313485 36.0 33.0 39.0 23.0 40.0 72-73 32.547568070667694 35.0 32.0 38.0 22.0 40.0 74-75 32.063232037747625 35.0 32.0 37.0 20.5 39.5 76-77 30.03821952282489 33.5 29.5 35.5 18.0 38.0 78-79 31.00973379722359 34.5 31.0 36.0 18.0 39.0 80-81 31.02675380803103 35.0 31.0 36.0 18.0 37.5 82-83 30.705991366550013 34.5 31.0 35.5 16.5 37.0 84-85 30.319166895372973 34.0 31.0 35.0 10.0 37.0 86-87 29.816965632686873 34.0 30.0 35.0 4.5 36.0 88-89 29.31506694418259 34.0 30.0 35.0 2.0 36.0 90-91 28.903290188811972 34.0 29.0 35.0 2.0 35.5 92-93 28.58218568224654 34.0 29.0 35.0 2.0 35.0 94-95 28.21978363531089 34.0 29.0 35.0 2.0 35.0 96-97 27.694385302792945 34.0 28.5 35.0 2.0 35.0 98-99 27.01286380638889 33.0 27.0 35.0 2.0 35.0 100-101 23.8026631073715 30.0 13.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.17410684061641746 1101 2 0.20554503284797931 1101 3 0.2492364053956564 1101 4 0.13918280286436868 1101 5 0.18275224897674036 1101 6 0.17083336518507508 1101 7 0.1689335650155286 1101 8 0.259232940600981 1101 9 0.35574647824207517 1101 10-11 0.186764136051373 1101 12-13 0.26891336061287063 1101 14-15 0.3533163859989159 1101 16-17 0.43494010525959226 1101 18-19 0.3670386451919754 1101 20-21 0.37567390880784046 1101 22-23 0.34003191755285656 1101 24-25 0.3478759546452963 1101 26-27 0.2967909336237895 1101 28-29 0.338499909514951 1101 30-31 0.35880986682276017 1101 32-33 0.3551800220240011 1101 34-35 0.4136248949747028 1101 36-37 0.5056978866609114 1101 38-39 0.5850404542111676 1101 40-41 0.5957565407733654 1101 42-43 0.592586493564589 1101 44-45 0.7188381920055775 1101 46-47 0.6181016328884752 1101 48-49 0.5383061725004623 1101 50-51 0.6636405633414384 1101 52-53 0.5431965844204569 1101 54-55 0.5521273466790291 1101 56-57 0.4738080547693144 1101 58-59 0.46303044857518927 1101 60-61 0.5584974052273353 1101 62-63 0.7204494165696858 1101 64-65 0.605089815854754 1101 66-67 0.6985054823928607 1101 68-69 0.6021167470515749 1101 70-71 0.6352985277452774 1101 72-73 0.39376703968458315 1101 74-75 0.4131380155697997 1101 76-77 0.4042002758626282 1101 78-79 0.6205504502649823 1101 80-81 0.7127639656402707 1101 82-83 0.680174244654733 1101 84-85 0.7702980462150144 1101 86-87 0.6765317946064755 1101 88-89 0.7875367492082646 1101 90-91 0.9290524740535702 1101 92-93 0.7557799034603256 1101 94-95 0.8191417354753376 1101 96-97 0.6433574573193148 1101 98-99 0.4721694683974924 1101 100-101 0.34583622767801714 1102 1 0.1856226450973537 1102 2 0.16834399601149386 1102 3 0.20558145615776624 1102 4 0.1390547075831492 1102 5 0.13683144514208578 1102 6 0.11710958505075553 1102 7 0.15599632860737245 1102 8 0.15639139252887446 1102 9 0.2936578053478911 1102 10-11 0.2809387419165077 1102 12-13 0.19387661545591328 1102 14-15 0.2709849822216981 1102 16-17 0.33095827698776503 1102 18-19 0.24048457079454266 1102 20-21 0.22867876868863846 1102 22-23 0.1932492375828545 1102 24-25 0.21960807374372848 1102 26-27 0.34037603029896246 1102 28-29 0.2985192824750058 1102 30-31 0.3346904154575512 1102 32-33 0.22585641928809963 1102 34-35 0.5046803226929697 1102 36-37 0.48864805643552955 1102 38-39 0.4018196976672783 1102 40-41 0.46013846749666243 1102 42-43 0.44034776730381964 1102 44-45 0.500137945025493 1102 46-47 0.5371245174394161 1102 48-49 0.4851812668304447 1102 50-51 0.5056754749169983 1102 52-53 0.6196296068421674 1102 54-55 0.4648118367785372 1102 56-57 0.42964652932867153 1102 58-59 0.35914091856490415 1102 60-61 0.2889754521728989 1102 62-63 0.3970390170904281 1102 64-65 0.45744665293071307 1102 66-67 0.2140771859517443 1102 68-69 0.38651207153510114 1102 70-71 0.34404913822958605 1102 72-73 0.5554474717565441 1102 74-75 0.4572933490935327 1102 76-77 0.5605614643082006 1102 78-79 0.5438171198728092 1102 80-81 0.5119866091708367 1102 82-83 0.4834175707357815 1102 84-85 0.44279691812515054 1102 86-87 0.45015279665986796 1102 88-89 0.49235394447113734 1102 90-91 0.5606190986925981 1102 92-93 0.442979547115506 1102 94-95 0.6267101155228119 1102 96-97 0.6785426382045578 1102 98-99 0.6179718905205931 1102 100-101 0.6018706377083021 1103 1 0.16231494557817427 1103 2 0.19489241251615397 1103 3 0.15109690183929558 1103 4 0.08045830068952853 1103 5 0.10680774547012817 1103 6 0.06820870893835007 1103 7 0.18568476839118375 1103 8 0.05522508163377893 1103 9 0.16412866223589617 1103 10-11 0.1846323491007169 1103 12-13 0.12716722790899126 1103 14-15 0.23895628181479367 1103 16-17 0.12859594862520396 1103 18-19 0.1395508970830477 1103 20-21 0.15491542050330054 1103 22-23 0.24215551489023568 1103 24-25 0.23939419413866858 1103 26-27 0.23037297862380512 1103 28-29 0.3187130001908116 1103 30-31 0.3361261688220125 1103 32-33 0.3869296984577346 1103 34-35 0.26382213453926084 1103 36-37 0.2821023096643813 1103 38-39 0.41530995371864066 1103 40-41 0.4097738675458231 1103 42-43 0.381590674859666 1103 44-45 0.5048977480979246 1103 46-47 0.5759446418289329 1103 48-49 0.43721171105173795 1103 50-51 0.4964675948821622 1103 52-53 0.5614012528061778 1103 54-55 0.5327878045472971 1103 56-57 0.6403882329967345 1103 58-59 0.4973648281890277 1103 60-61 0.43669967795982956 1103 62-63 0.3681854477300206 1103 64-65 0.4748994570961145 1103 66-67 0.4342325937631841 1103 68-69 0.33126652742451057 1103 70-71 0.4310153217336392 1103 72-73 0.5335849776930388 1103 74-75 0.44416555180500694 1103 76-77 0.4643432267104046 1103 78-79 0.6146707671647462 1103 80-81 0.4730729283683459 1103 82-83 0.3637413646585905 1103 84-85 0.4507582795194942 1103 86-87 0.5258767786214165 1103 88-89 0.5355739208679147 1103 90-91 0.510480191531812 1103 92-93 0.5413297135301818 1103 94-95 0.4894707665828548 1103 96-97 0.47026117884797003 1103 98-99 0.5391553386357479 1103 100-101 0.5300166119469161 1104 1 0.05162293455059341 1104 2 0.05195827672019249 1104 3 0.0710553977491486 1104 4 0.010774364504889888 1104 5 0.08826347695146808 1104 6 0.07084772127659278 1104 7 0.1277293156124415 1104 8 0.06795162165396817 1104 9 0.09831228958792337 1104 10-11 0.15807688728298075 1104 12-13 0.12364973743760999 1104 14-15 0.11680733410542388 1104 16-17 0.09436072362934311 1104 18-19 0.033335075814278525 1104 20-21 0.06724612750400638 1104 22-23 0.06929907686014047 1104 24-25 -0.005957121789563757 1104 26-27 0.07724247048116695 1104 28-29 0.043998670357581204 1104 30-31 0.1327308932077429 1104 32-33 0.043652080536404014 1104 34-35 0.10566179768573392 1104 36-37 0.2185598112838676 1104 38-39 0.23545073619503398 1104 40-41 0.18523768140261154 1104 42-43 0.18464039691031076 1104 44-45 0.20162977178011232 1104 46-47 0.21004597658842528 1104 48-49 0.3023951651561916 1104 50-51 0.25224069330184307 1104 52-53 0.21561211015564652 1104 54-55 0.2655933605213221 1104 56-57 0.2533234233430832 1104 58-59 0.3567220797294155 1104 60-61 0.4039299013738571 1104 62-63 0.3924372818944377 1104 64-65 0.14304921078327482 1104 66-67 0.27480999776143733 1104 68-69 0.19441319278553948 1104 70-71 0.13412812849482236 1104 72-73 0.16193731804164457 1104 74-75 0.22906156560456026 1104 76-77 0.20470727256828525 1104 78-79 0.36091637948189614 1104 80-81 0.2995865847958292 1104 82-83 0.18658575068429073 1104 84-85 0.3399069560587584 1104 86-87 0.49291322840222307 1104 88-89 0.610204639920628 1104 90-91 0.5441278813132158 1104 92-93 0.3335766967628295 1104 94-95 0.4302185934558125 1104 96-97 0.5231361468348332 1104 98-99 0.8707308369108056 1104 100-101 0.9553958734603469 1105 1 -0.003467061536532867 1105 2 0.010772023948575793 1105 3 0.024844001143200956 1105 4 0.05193706757577132 1105 5 0.045397949365714396 1105 6 -0.03942248436663931 1105 7 -0.01950450030860651 1105 8 -0.05674114532718022 1105 9 -0.10543211282936227 1105 10-11 -0.03904026704364583 1105 12-13 0.027071146651699962 1105 14-15 0.048826594137601376 1105 16-17 0.03719654236186187 1105 18-19 -0.009358720107641716 1105 20-21 0.07832970358041536 1105 22-23 0.08080781561063333 1105 24-25 0.09462509927330132 1105 26-27 0.00295656986814663 1105 28-29 0.01545588960891564 1105 30-31 -0.049094896975297786 1105 32-33 0.049807247703043345 1105 34-35 0.16019457162310147 1105 36-37 0.04692361430692671 1105 38-39 -0.05771224222620219 1105 40-41 0.03261804892286335 1105 42-43 -0.03421729768267312 1105 44-45 0.17766161097550537 1105 46-47 0.10973566399331247 1105 48-49 0.14058028029611336 1105 50-51 0.15786997753586718 1105 52-53 0.2474344584593382 1105 54-55 0.08392299836314976 1105 56-57 0.18727665726044762 1105 58-59 0.06909436789080559 1105 60-61 -0.1519180327739349 1105 62-63 -0.2709659397046167 1105 64-65 -0.23004924145793382 1105 66-67 -0.07953238590304323 1105 68-69 0.16094635076418484 1105 70-71 0.1192342108390676 1105 72-73 -0.14655361713509052 1105 74-75 -0.040026916055659 1105 76-77 0.08971770689722547 1105 78-79 0.12080888415560409 1105 80-81 0.24958803400818397 1105 82-83 0.23733010237914343 1105 84-85 0.030831988303734903 1105 86-87 -0.03598424706985526 1105 88-89 0.12960216611514142 1105 90-91 0.07526420871927542 1105 92-93 -0.0991155412558058 1105 94-95 -0.027664397138799757 1105 96-97 0.1065471941806031 1105 98-99 0.36100589741542066 1105 100-101 0.460152913009356 1106 1 0.08825444027193896 1106 2 0.09871783832046788 1106 3 0.18761393149513594 1106 4 0.19252427222515678 1106 5 0.10426194368817221 1106 6 0.11442718122718532 1106 7 0.127431890926907 1106 8 0.13769309642610494 1106 9 0.21152745753703783 1106 10-11 0.2573762711382841 1106 12-13 0.2209335189191961 1106 14-15 0.19790347539279907 1106 16-17 0.18569636632221176 1106 18-19 0.08991675047851544 1106 20-21 0.17352824795035815 1106 22-23 0.23641874238159488 1106 24-25 0.1172909829266473 1106 26-27 0.19711810579165245 1106 28-29 0.18254640027608815 1106 30-31 0.22750715034167257 1106 32-33 0.1592409672168884 1106 34-35 0.24817791114721643 1106 36-37 0.34962933341681435 1106 38-39 0.4556408324498875 1106 40-41 0.34866473782102503 1106 42-43 0.36055716058901055 1106 44-45 0.4069131415521028 1106 46-47 0.3897284633143343 1106 48-49 0.30892537612187 1106 50-51 0.3410077650528933 1106 52-53 0.4323385220355931 1106 54-55 0.34859827264261867 1106 56-57 0.3269051547948507 1106 58-59 0.2442394709103155 1106 60-61 0.33936886897657104 1106 62-63 0.29102007811238906 1106 64-65 0.3143526975375863 1106 66-67 0.3196100346733317 1106 68-69 0.4641450846620856 1106 70-71 0.503937748302306 1106 72-73 0.51669895238674 1106 74-75 0.4925667048759692 1106 76-77 0.46114200950005113 1106 78-79 0.5648464314424864 1106 80-81 0.509071521903639 1106 82-83 0.4779387845580523 1106 84-85 0.5832401001003298 1106 86-87 0.6249964079359955 1106 88-89 0.7972581479702612 1106 90-91 0.8795150302953019 1106 92-93 0.851732734696899 1106 94-95 0.9021825901621341 1106 96-97 0.9343051891131502 1106 98-99 0.9046855204622979 1106 100-101 0.6236076341576968 1107 1 0.04055449831444946 1107 2 0.049152432447833405 1107 3 0.019486319664480334 1107 4 0.018133537417504897 1107 5 0.04625138535347162 1107 6 0.17922660515031907 1107 7 0.0388136255217546 1107 8 0.10675136491455817 1107 9 0.004294938972989826 1107 10-11 0.0524054999239425 1107 12-13 0.12969329341645874 1107 14-15 0.16041334953740005 1107 16-17 0.019706148560672432 1107 18-19 0.08867898239425642 1107 20-21 0.11246815188044934 1107 22-23 0.04946371445728204 1107 24-25 0.04547737515303396 1107 26-27 0.14023265641019123 1107 28-29 0.13817665564337034 1107 30-31 0.16968583958233552 1107 32-33 0.12587805262634788 1107 34-35 0.15010566699709926 1107 36-37 0.25400693124743157 1107 38-39 -0.027515639224247934 1107 40-41 0.029914997233490226 1107 42-43 0.039033435959552776 1107 44-45 0.16467344508611603 1107 46-47 0.0805145605187434 1107 48-49 0.1471000873751933 1107 50-51 0.06693264051843073 1107 52-53 0.27686085061418453 1107 54-55 0.1987555342483205 1107 56-57 0.21043241336337104 1107 58-59 0.226908905961011 1107 60-61 0.11999716433850693 1107 62-63 0.09761399728638054 1107 64-65 0.10570518687615049 1107 66-67 0.25739122955847193 1107 68-69 0.4725190839887645 1107 70-71 0.3431219446638778 1107 72-73 0.12855843416791402 1107 74-75 0.25798667637518946 1107 76-77 0.4005590676736688 1107 78-79 0.6331776532406437 1107 80-81 0.6577387512071375 1107 82-83 0.651861918089427 1107 84-85 0.7108484719255337 1107 86-87 0.5780256209098482 1107 88-89 0.7222332608435096 1107 90-91 0.7698239073736488 1107 92-93 0.7944541313011833 1107 94-95 0.910787837178809 1107 96-97 1.0199098227634416 1107 98-99 1.3803040421504988 1107 100-101 1.0432635560420955 1108 1 0.03589122723352034 1108 2 0.08298713278340131 1108 3 0.03166515384670987 1108 4 0.04137209724277824 1108 5 0.053893844522754364 1108 6 0.03167389784821495 1108 7 0.13381828847400357 1108 8 0.05726285490714389 1108 9 0.0653906131880575 1108 10-11 0.011139408753258806 1108 12-13 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48-49 -0.7743283291195624 2316 50-51 -0.6786142096349437 2316 52-53 -0.932216411496924 2316 54-55 -0.781892951076486 2316 56-57 -0.6651808076175953 2316 58-59 -0.5387607475478333 2316 60-61 -0.7702985705026961 2316 62-63 -0.8312747638994864 2316 64-65 -0.7057499430245855 2316 66-67 -0.8158134467383107 2316 68-69 -0.7520464886480056 2316 70-71 -0.6924895917640157 2316 72-73 -0.6605614375581581 2316 74-75 -0.7202851242909176 2316 76-77 -0.9487894919911994 2316 78-79 -0.9409315548137762 2316 80-81 -0.8756996272499649 2316 82-83 -0.9512751253734528 2316 84-85 -1.2157426208451731 2316 86-87 -1.0710676078347134 2316 88-89 -1.559550213240147 2316 90-91 -2.0049014865314803 2316 92-93 -1.708328479941997 2316 94-95 -1.3721208789185013 2316 96-97 -1.3335898362413232 2316 98-99 -1.503659406991634 2316 100-101 -0.7055050722838025 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 5.0 4 11.0 5 51.0 6 88.0 7 217.0 8 537.0 9 993.0 10 1758.0 11 2844.0 12 4291.0 13 6002.0 14 7258.0 15 9798.0 16 13233.0 17 18269.0 18 16457.0 19 10464.0 20 9156.0 21 9451.0 22 9965.0 23 11023.0 24 12744.0 25 14945.0 26 17482.0 27 21344.0 28 26166.0 29 31893.0 30 39015.0 31 47680.0 32 58962.0 33 75176.0 34 102337.0 35 144150.0 36 224012.0 37 387325.0 38 385293.0 39 39501.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.113551996945965 21.077003284797343 15.46777736310258 21.34166735515411 2 14.870285169773947 22.629366798890164 40.04332066783378 22.45702736350211 3 19.198772202268426 26.963195046988492 31.07541966896964 22.76261308177344 4 12.176323754942755 17.953871216473217 39.416693798905506 30.45311122967852 5 12.115013418383668 39.63216070922252 34.67875145107759 13.574074421316224 6 28.745967808379913 38.56840648241746 18.098595430760515 14.587030278442114 7 26.2988955616203 34.726197355643706 22.002399001306326 16.972508081429673 8 24.773126185082212 35.06297804589922 20.830740855014977 19.333154914003586 9 25.79687811630099 17.823693291490024 22.185818618000237 34.19360997420875 10-11 23.960664788149774 27.60658424148204 27.967059473299322 20.465691497068867 12-13 25.72440236627215 25.634709719137295 28.10399914995122 20.536888764639336 14-15 22.37179565492982 26.936003020700838 27.859146470180953 22.833054854188394 16-17 20.301246614129205 30.120911172137752 28.457707796011906 21.120134417721136 18-19 20.08865281473539 29.1122388273418 31.032519479811054 19.766588878111754 20-21 21.70167151637679 27.42700007216323 30.461236695969486 20.41009171549049 22-23 21.034957119698348 27.585446664837015 30.53908207232347 20.84051414314117 24-25 21.216235604630384 27.74317822486821 30.82986367103766 20.21072249946375 26-27 20.872091817441323 27.50395125586438 31.35093885475942 20.273018071934874 28-29 20.192891751174358 28.632722673289773 31.028911318206333 20.14547425732954 30-31 20.72579165054358 27.80440809387357 30.656901333542436 20.81289892204041 32-33 20.521433332253725 28.119710278828503 30.612694251204186 20.746162137713583 34-35 21.33110281992506 27.613911312089662 30.65468106650968 20.400304801475595 36-37 21.15604626935823 27.3200179821463 30.96856363912265 20.555372109372826 38-39 20.694721469282918 28.13177290214062 30.78757451257835 20.385931115998112 40-41 20.7347935772908 28.120494280133375 30.69251617867785 20.452195963897974 42-43 20.474951294222674 28.254067291112094 29.860498470992326 21.41048294367291 44-45 20.839047473746206 27.797329205799116 29.891041392679142 21.47258192777554 46-47 21.47541672917036 28.21649688390678 29.524102560605854 20.78398382631701 48-49 21.2131491636736 28.201959316983533 29.52839907290134 21.05649244644153 50-51 20.510921365373807 28.450922467709795 29.905409344513522 21.132746822402876 52-53 21.032854726322363 28.898987953285953 28.85049085203185 21.217666468359834 54-55 20.534331799722597 28.243382148634666 29.234717446853487 21.98756860478925 56-57 21.184937317425604 28.30088544853994 28.799607250188792 21.714569983845664 58-59 21.60480779229192 28.040511553827418 28.92653132889329 21.428149324987373 60-61 21.064458160793812 28.523989800339518 29.564525637656352 20.84702640121032 62-63 20.71526528677974 28.375424780744808 29.554881203446715 21.354428729028736 64-65 20.43955363347556 28.715568336592046 29.22014274682809 21.624735283104314 66-67 20.698602590262283 28.351144014514468 28.59399886016186 22.35625453506139 68-69 20.462967356133476 28.3045241838039 29.06511300586714 22.167395454195486 70-71 21.07214675387622 28.113090580766283 28.77974815600213 22.035014509355367 72-73 20.83846857120778 27.78108289168867 29.181390522978873 22.199058014124674 74-75 20.34329810972107 28.957570860600523 28.50311296273252 22.19601806694589 76-77 20.567403757328474 27.986008832330384 28.8117474322568 22.63483997808434 78-79 20.455776155336185 28.27139511983358 28.808641859561256 22.464186865268974 80-81 20.85833271302483 28.27978887160864 28.418690624401595 22.443187790964934 82-83 20.766276928391914 27.967485634118777 28.829239632501636 22.436997804987673 84-85 20.355003326895464 28.201760441934454 28.543086847597504 22.90014938357258 86-87 20.304197065137387 28.360735092187994 28.483969060964554 22.851098781710068 88-89 20.3235889034052 28.094820718494645 28.20436785712453 23.377222520975625 90-91 20.25594263545468 27.719087546056386 28.503664266402517 23.521305552086417 92-93 20.75437283616844 28.200055798656624 28.244575398929143 22.80099596624579 94-95 20.283334602346272 27.703651007046997 28.43403424516648 23.578980145440248 96-97 19.792982438196738 27.73596666626892 28.826341738929333 23.64470915660501 98-99 19.989641451015085 28.017687378650706 27.994106479974135 23.998564690360073 100-101 19.71409065378104 27.456405742733864 28.665314122701176 24.164189480783925 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 809.0 1 542.5 2 348.5 3 431.5 4 404.5 5 421.0 6 724.0 7 1516.5 8 2389.5 9 2613.0 10 2300.0 11 2111.0 12 2212.5 13 2364.0 14 2585.0 15 2825.0 16 2967.5 17 3156.5 18 3403.0 19 3747.5 20 4128.5 21 4688.0 22 5463.5 23 6492.5 24 7773.5 25 9304.5 26 11733.0 27 14837.5 28 17364.0 29 20219.5 30 24907.5 31 29752.0 32 35022.0 33 40356.0 34 45002.5 35 49896.5 36 55908.5 37 61045.5 38 61817.5 39 61805.0 40 62187.5 41 61691.5 42 60382.0 43 61412.5 44 64285.0 45 65608.5 46 64666.5 47 71121.5 48 90831.0 49 87849.5 50 65126.0 51 52372.0 52 45454.5 53 42735.0 54 40888.0 55 36953.0 56 32369.0 57 28662.0 58 24804.0 59 20778.5 60 17286.0 61 13992.5 62 11060.5 63 8128.5 64 5696.5 65 4406.5 66 3583.5 67 2941.0 68 2178.0 69 1343.5 70 879.5 71 702.5 72 587.0 73 536.5 74 414.0 75 332.5 76 238.0 77 138.5 78 103.5 79 81.0 80 48.5 81 27.5 82 15.5 83 6.0 84 3.0 85 0.5 86 0.0 87 1.0 88 2.0 89 1.5 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.27524306792605713 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.002414911310251327 16-17 6.818573111297864E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 8.52321638912233E-5 26-27 8.807323602093074E-4 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0036933937686196763 36-37 0.021364862415399973 38-39 0.007329966094645204 40-41 0.018751076056069127 42-43 0.005540090652929514 44-45 5.966251472385632E-4 46-47 1.704643277824466E-4 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 3.409286555648932E-4 60-61 0.002073982654686434 62-63 0.0011648395731800518 64-65 0.0 66-67 0.0 68-69 7.102680324268609E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.23166102145634493 78-79 0.0 80-81 0.0012500717370712753 82-83 0.0 84-85 0.0 86-87 0.10574470466771105 88-89 0.35888423142464426 90-91 0.36036158893209214 92-93 0.0 94-95 5.682144259414887E-5 96-97 0.0 98-99 0.0 100-101 3.125179342678188E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1759899.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.802166996010556 #Duplication Level Percentage of deduplicated Percentage of total 1 66.06615965656768 25.635101597792342 2 15.131517837544713 11.74271364071045 3 6.807043868163326 7.923841587649291 4 3.585061031183301 5.56432547291457 5 2.122318840900226 4.117528504169505 6 1.4060886668809212 3.2735572358106815 7 0.9431407414091226 2.561713318422859 8 0.6578565394953236 2.042100744393213 9 0.5016905181822099 1.7520011340139057 >10 2.47282728129943 17.282287899581235 >50 0.19791082131046267 5.323409851432475 >100 0.10090458862910352 6.825660253226347 >500 0.00307983876704503 0.7471302409610893 >1k 0.0036664747226726547 2.9967724593839535 >5k 5.866359556276246E-4 1.5211774294409992 >10k+ 1.4665898890690616E-4 0.6906786300970935 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 12137 0.6896418487651849 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8737 0.49644894394507866 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 6464 0.3672938049285783 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6360 0.36138437489878683 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5170 0.29376685821174964 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4707 0.2674585302906587 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4292 0.24387763161408693 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3268 0.1856924743976785 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3167 0.17995350869566948 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3142 0.17853297263081574 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3064 0.17410090010847212 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2865 0.1627934330322365 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2541 0.14438328563173228 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2441 0.13870114137231737 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2002 0.11375652807348603 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1958 0.11125638459934348 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.8410721297074434E-5 0.0 12-13 0.0 0.0 0.0 1.1364288518829774E-4 0.0 14-15 0.0 0.0 0.0 2.2728577037659547E-4 0.0 16-17 0.0 0.0 0.0 3.977500981590421E-4 0.0 18-19 0.0 0.0 0.0 5.682144259414887E-4 0.0 20-21 0.0 0.0 0.0 6.53446589832712E-4 0.0 22-23 0.0 0.0 0.0 0.001051196687991754 0.0 24-25 0.0 0.0 0.0 0.0017614647204186149 0.0 26-27 0.0 0.0 0.0 0.00326723294916356 0.0 28-29 0.0 0.0 0.0 0.008153877012260363 0.0 30-31 0.0 0.0 0.0 0.023211559299709812 0.0 32-33 0.0 0.0 0.0 0.055486138693186374 0.0 34-35 0.0 0.0 0.0 0.10043189978515812 0.0 36-37 0.0 0.0 0.0 0.1740156679445809 0.0 38-39 0.0 0.0 0.0 0.2820900517586521 0.0 40-41 0.0 0.0 0.0 0.40189806346841495 0.0 42-43 0.0 0.0 0.0 0.530200880846003 0.0 44-45 0.0 0.0 0.0 0.6858916335539711 0.0 46-47 0.0 0.0 0.0 0.8789140740462947 0.0 48-49 0.0 0.0 0.0 1.0747491759470287 0.0 50-51 0.0 0.0 0.0 1.2881705143306519 0.0 52-53 0.0 0.0 0.0 1.4978984589456554 0.0 54-55 0.0 0.0 0.0 1.7335369813835908 0.0 56-57 0.0 0.0 0.0 2.020968248746093 0.0 58-59 0.0 0.0 0.0 2.33524764773433 0.0 60-61 0.0 0.0 0.0 2.673363641890813 0.0 62-63 0.0 0.0 0.0 3.019093709354912 0.0 64-65 0.0 0.0 0.0 3.383631674317674 0.0 66-67 0.0 0.0 0.0 3.8062979750542505 0.0 68-69 0.0 0.0 0.0 4.282035503173761 0.0 70-71 0.0 0.0 0.0 4.7905874143913945 0.0 72-73 0.0 0.0 0.0 5.298372236134005 0.0 74-75 0.0 0.0 0.0 5.838346405106202 0.0 76-77 0.0 0.0 0.0 6.417555780189659 0.0 78-79 0.0 0.0 0.0 7.096572019189738 0.0 80-81 0.0 0.0 0.0 7.831443736259866 0.0 82-83 0.0 0.0 0.0 8.557735415498275 0.0 84-85 0.0 0.0 0.0 9.304113474693718 0.0 86-87 0.0 0.0 0.0 10.086999310755902 0.0 88-89 0.0 0.0 0.0 10.928723750624325 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGATCG 55 0.0025914502 34.514263 5 CCGTGCG 70 2.064355E-4 33.898903 9 GCGTCCG 85 1.6424983E-5 33.50009 9 GTACATG 10420 0.0 25.978157 1 TACATGG 10380 0.0 25.603067 2 CATGGGG 3565 0.0 25.159592 4 ACATGGG 10415 0.0 24.560137 3 GAGTACT 7445 0.0 21.865297 12-13 GTATAGG 610 0.0 21.836948 1 AGTACTT 7945 0.0 20.997007 12-13 CATGGGA 5800 0.0 20.619299 4 GTACTTT 7865 0.0 20.546808 14-15 ATGGGGG 1820 0.0 18.513489 5 ATGGGGA 2215 0.0 18.425787 5 GTACACG 240 3.0467872E-6 17.840006 1 ATACCGT 160 0.001198595 17.79642 6 CCATGCG 135 0.009449809 17.577208 9 TACTTTT 9490 0.0 17.503618 14-15 AGAGTAC 15285 0.0 17.418758 10-11 GTCCCGA 165 0.001473116 17.257133 6 >>END_MODULE