##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939291_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 793520 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.88339676378667 33.0 31.0 34.0 30.0 34.0 2 31.853539923379373 33.0 31.0 34.0 30.0 34.0 3 31.902022633329974 34.0 31.0 34.0 30.0 34.0 4 35.33348497832443 37.0 35.0 37.0 33.0 37.0 5 35.41539091642303 37.0 35.0 37.0 33.0 37.0 6 35.57328611755217 37.0 35.0 37.0 33.0 37.0 7 35.54969881036395 37.0 35.0 37.0 33.0 37.0 8 35.57900115939107 37.0 35.0 37.0 33.0 37.0 9 37.101226182074804 39.0 37.0 39.0 33.0 39.0 10-11 37.031824654703094 39.0 37.0 39.0 33.0 39.0 12-13 36.940166599455594 39.0 37.0 39.0 33.0 39.0 14-15 37.829468696441175 40.0 37.0 41.0 33.0 41.0 16-17 37.81613191854018 40.0 37.5 41.0 32.5 41.0 18-19 37.98454481298518 40.0 38.0 41.0 33.0 41.0 20-21 37.99368698961589 40.0 38.0 41.0 33.0 41.0 22-23 38.065464638572436 40.0 38.0 41.0 33.0 41.0 24-25 37.96539406694223 40.0 38.0 41.0 33.0 41.0 26-27 37.625297409013 40.0 37.0 41.0 32.5 41.0 28-29 37.38440429982862 39.5 36.5 41.0 31.5 41.0 30-31 37.27607873777598 39.0 36.5 41.0 32.0 41.0 32-33 37.042295720334714 39.0 35.0 41.0 31.5 41.0 34-35 36.7749785764694 39.0 35.0 41.0 31.0 41.0 36-37 36.35801996168969 39.0 35.0 41.0 30.0 41.0 38-39 35.47825196592399 38.5 34.0 40.0 27.5 41.0 40-41 35.361433864300835 38.0 35.0 40.0 29.0 41.0 42-43 35.66877835467285 38.5 35.0 40.0 29.5 41.0 44-45 35.568988179251946 39.0 35.0 41.0 28.0 41.0 46-47 35.08020528783143 39.0 35.0 41.0 24.5 41.0 48-49 34.75143096582317 38.5 35.0 40.0 23.5 41.0 50-51 33.527112108075414 37.5 33.5 39.5 21.5 40.5 52-53 33.42171148805323 37.5 33.5 39.5 21.0 40.0 54-55 33.94579216654905 38.0 34.0 40.0 21.0 41.0 56-57 34.048649057364656 38.0 34.0 40.0 20.5 41.0 58-59 33.936317295090234 38.0 34.0 40.0 19.5 41.0 60-61 33.504905358403064 38.0 33.5 40.0 15.0 41.0 62-63 32.9065014114326 37.0 32.5 40.0 10.0 41.0 64-65 32.176316917027926 36.0 31.0 39.0 7.5 41.0 66-67 31.782519029136 36.0 31.0 39.0 2.0 40.5 68-69 31.499383758443393 35.5 31.0 38.5 2.0 40.0 70-71 30.894765727391874 35.0 30.5 37.5 2.0 39.5 72-73 30.210470435527775 35.0 29.5 37.0 2.0 39.0 74-75 29.735018020969854 35.0 29.0 36.5 2.0 39.0 76-77 29.070451280371003 34.0 28.5 36.0 2.0 37.5 78-79 28.631211563665694 34.0 28.5 35.0 2.0 37.0 80-81 28.34140034277649 34.0 28.5 35.0 2.0 37.0 82-83 27.93011770339752 34.0 27.0 35.0 2.0 36.0 84-85 27.51771536949289 34.0 26.0 35.0 2.0 36.0 86-87 26.865968091541486 33.5 25.0 35.0 2.0 35.5 88-89 26.12000579695534 32.5 23.5 35.0 2.0 35.0 90-91 25.457326217360624 32.0 20.0 35.0 2.0 35.0 92-93 25.069288738784152 32.0 18.5 35.0 2.0 35.0 94-95 24.63061044460127 32.0 12.0 35.0 2.0 35.0 96-97 23.726592272406492 31.5 2.0 34.0 2.0 35.0 98-99 22.738610242968043 31.0 2.0 34.0 2.0 35.0 100-101 20.226141118056255 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.38549436643321755 1101 2 0.49239366704588505 1101 3 0.5488387141886868 1101 4 0.3089076182444188 1101 5 0.2626732648746213 1101 6 0.2579323525090942 1101 7 0.261434996348342 1101 8 0.2707612573307969 1101 9 0.3181627313042199 1101 10-11 0.5120309017459945 1101 12-13 0.51959608882526 1101 14-15 0.7704867874214258 1101 16-17 0.8740392570396622 1101 18-19 0.8385175911772578 1101 20-21 0.8413048397706646 1101 22-23 0.876118243194874 1101 24-25 0.7310294622287543 1101 26-27 0.749226526949748 1101 28-29 0.7994673584728744 1101 30-31 0.7743858682004046 1101 32-33 0.7307055835719964 1101 34-35 0.7770479394855343 1101 36-37 0.8603942703291736 1101 38-39 1.186950517937582 1101 40-41 0.9240964887110934 1101 42-43 0.7884628055049205 1101 44-45 0.8123949213473338 1101 46-47 1.2424698407312889 1101 48-49 1.3615783093965135 1101 50-51 1.2486307698375185 1101 52-53 1.4083427767570882 1101 54-55 1.3393757399446997 1101 56-57 1.5084052335334164 1101 58-59 1.5444323739351162 1101 60-61 1.52979149547307 1101 62-63 1.3931632043979505 1101 64-65 1.456773581739128 1101 66-67 1.2222933850842423 1101 68-69 1.387040223329464 1101 70-71 1.451794188050794 1101 72-73 1.373249383757404 1101 74-75 1.2001551945774267 1101 76-77 1.2464660608013993 1101 78-79 1.4918762022723797 1101 80-81 1.3409723245234382 1101 82-83 1.2808145925481291 1101 84-85 1.3573298157971685 1101 86-87 1.0937242244193435 1101 88-89 1.136644984904823 1101 90-91 1.3630546319329895 1101 92-93 1.427635262152453 1101 94-95 1.647780474484172 1101 96-97 1.8824721421448949 1101 98-99 1.728695943956776 1101 100-101 1.2666854077500886 1102 1 0.4778203623654953 1102 2 0.55272038352226 1102 3 0.563262163709755 1102 4 0.595757479057923 1102 5 0.5295615407424705 1102 6 0.5712014652553918 1102 7 0.4920761339701727 1102 8 0.5900544538445374 1102 9 0.6217128771934526 1102 10-11 0.6578949995891819 1102 12-13 0.6116016102038415 1102 14-15 0.6385570488490657 1102 16-17 0.6533946214276014 1102 18-19 0.7917953336958448 1102 20-21 0.8353823443421362 1102 22-23 0.7363609660136987 1102 24-25 0.6549198976342794 1102 26-27 0.5707043964014034 1102 28-29 0.7626829540633153 1102 30-31 0.5787895655970203 1102 32-33 0.49047324999359176 1102 34-35 0.43211049486165365 1102 36-37 0.6740278443326062 1102 38-39 1.1270364386316558 1102 40-41 0.7935230068283872 1102 42-43 0.5816970221331559 1102 44-45 0.8344196921451896 1102 46-47 0.844027807875321 1102 48-49 0.9143864414906773 1102 50-51 0.6146598387646875 1102 52-53 0.7472959351071324 1102 54-55 1.0132070027610638 1102 56-57 1.007035361911754 1102 58-59 0.8528440978578047 1102 60-61 0.7746413856110408 1102 62-63 0.9300146361651471 1102 64-65 0.7894481618232092 1102 66-67 0.5171425421104914 1102 68-69 0.2365271498528152 1102 70-71 0.20675578346947887 1102 72-73 0.4019056737512656 1102 74-75 0.4538642162503024 1102 76-77 0.3030664751142531 1102 78-79 0.2513391036772319 1102 80-81 0.4346189688616171 1102 82-83 0.40642595536092685 1102 84-85 0.32472414821515727 1102 86-87 0.4627360107612617 1102 88-89 0.6000951762469775 1102 90-91 0.6796834909807394 1102 92-93 0.9275912754490037 1102 94-95 0.8422278862855919 1102 96-97 0.8098186756784713 1102 98-99 0.5030200673122494 1102 100-101 0.30779936054188184 1103 1 0.45109479684051834 1103 2 0.45797331073712044 1103 3 0.5809297355500966 1103 4 0.42422498248882334 1103 5 0.2995452309311375 1103 6 0.38240809930243813 1103 7 0.4415824597576119 1103 8 0.2598788000964092 1103 9 0.3660638843555901 1103 10-11 0.5215683361761378 1103 12-13 0.5331716485381648 1103 14-15 0.6622980440395452 1103 16-17 0.7206027237747961 1103 18-19 0.7072617067024822 1103 20-21 0.7932844687889187 1103 22-23 0.7471886367974534 1103 24-25 0.8182755891744407 1103 26-27 0.7503208864132063 1103 28-29 0.8284801117345566 1103 30-31 0.7832691324484742 1103 32-33 0.822695972772685 1103 34-35 0.8186947364971431 1103 36-37 0.7360499689462401 1103 38-39 1.1718832056599737 1103 40-41 1.029647153274638 1103 42-43 1.0272593769195169 1103 44-45 0.9867068199081572 1103 46-47 1.0609712407777465 1103 48-49 0.7336666407123076 1103 50-51 0.833611756106059 1103 52-53 1.03863880404883 1103 54-55 0.7184551329725934 1103 56-57 0.8001437366453175 1103 58-59 0.9134730058512801 1103 60-61 1.0587981349947384 1103 62-63 1.0208333803513838 1103 64-65 1.1215572164798502 1103 66-67 0.8933986270049346 1103 68-69 0.8791076456544005 1103 70-71 0.9903076933024977 1103 72-73 0.9998771670375461 1103 74-75 0.9620097364748865 1103 76-77 0.9019170714026217 1103 78-79 0.7714667039658849 1103 80-81 0.5689475761038416 1103 82-83 0.8641143003113534 1103 84-85 1.135135105405034 1103 86-87 1.1810100906751657 1103 88-89 1.0765836029550258 1103 90-91 1.1760200403919612 1103 92-93 0.9243491268920678 1103 94-95 1.1134212555755951 1103 96-97 0.9799496130129235 1103 98-99 1.1985062957413781 1103 100-101 0.8888593314261577 1104 1 0.2125791037793725 1104 2 0.242438004292719 1104 3 0.044363608074874605 1104 4 0.21328104427215777 1104 5 0.19374362512952814 1104 6 0.14283285972719995 1104 7 0.16800754981905897 1104 8 0.06035441875384606 1104 9 0.23502152604050508 1104 10-11 0.193515076304692 1104 12-13 0.29682802583089796 1104 14-15 0.14991312256371714 1104 16-17 0.26465631328293426 1104 18-19 0.35742299322740934 1104 20-21 0.3235462945052916 1104 22-23 0.24171431314130842 1104 24-25 0.3952457570537078 1104 26-27 0.1272074678452455 1104 28-29 0.3852835776289254 1104 30-31 0.4240063413675017 1104 32-33 0.32532717994934757 1104 34-35 -0.0354527994685796 1104 36-37 -0.05091054528700312 1104 38-39 0.6460479298246966 1104 40-41 0.5527040626951845 1104 42-43 0.18958778924793762 1104 44-45 0.38099350964939305 1104 46-47 0.1726238978849608 1104 48-49 0.1058676720042584 1104 50-51 -0.08413800255279824 1104 52-53 0.016070463298667903 1104 54-55 0.3325212015841146 1104 56-57 0.3089615181903653 1104 58-59 0.004623437910801442 1104 60-61 -0.023563947367350124 1104 62-63 0.20324608549136514 1104 64-65 0.24066201740283333 1104 66-67 0.3745563581626641 1104 68-69 -0.03805347605218046 1104 70-71 0.07896819105390662 1104 72-73 0.44836554734410683 1104 74-75 0.2613080812277744 1104 76-77 0.16125873983520123 1104 78-79 0.017293267974267224 1104 80-81 -0.029760954422872743 1104 82-83 0.14071480418458648 1104 84-85 -0.033615336072681856 1104 86-87 0.012418581174568288 1104 88-89 0.2572127381296134 1104 90-91 0.3786406521034813 1104 92-93 0.2185969665944505 1104 94-95 0.28540920029081107 1104 96-97 0.22525817650330637 1104 98-99 0.4225827243632594 1104 100-101 0.4387168812837068 1105 1 0.2790058344289754 1105 2 0.2498582477833473 1105 3 0.27856782028495175 1105 4 0.3442461202518956 1105 5 0.30334773070137544 1105 6 0.430956026882626 1105 7 0.3675543373423906 1105 8 0.47292217846236184 1105 9 0.5135916851091977 1105 10-11 0.48303787022631894 1105 12-13 0.5860620078801304 1105 14-15 0.5685681478903035 1105 16-17 0.6002668171485652 1105 18-19 0.5722212692426965 1105 20-21 0.6939197504725811 1105 22-23 0.6306494859841081 1105 24-25 0.40603176967255905 1105 26-27 0.4387743833344473 1105 28-29 0.43186560020068754 1105 30-31 0.389360024829692 1105 32-33 0.5044405275011101 1105 34-35 0.5405436014911658 1105 36-37 0.4446073166164908 1105 38-39 0.934048907341797 1105 40-41 0.5658283406655045 1105 42-43 0.54790890361744 1105 44-45 0.4723469265160034 1105 46-47 0.7052651935394465 1105 48-49 0.5357585899816826 1105 50-51 0.4829718792565032 1105 52-53 0.439951872700604 1105 54-55 0.42500209556794033 1105 56-57 0.30411281117890354 1105 58-59 0.28636497350131407 1105 60-61 0.19637740160625583 1105 62-63 0.022016635566899367 1105 64-65 0.09947744440007256 1105 66-67 0.2185812848488844 1105 68-69 0.16320952048207715 1105 70-71 0.03855119114276917 1105 72-73 -0.046924597506151855 1105 74-75 -0.03394651754588551 1105 76-77 0.37872297159561796 1105 78-79 0.3824514480151464 1105 80-81 0.3861059255202548 1105 82-83 0.12308061774541201 1105 84-85 0.2616170464031029 1105 86-87 0.3499341325830798 1105 88-89 0.27246978069604566 1105 90-91 0.2656430070310556 1105 92-93 0.20706670799674498 1105 94-95 0.2616210993399548 1105 96-97 0.45362505644643036 1105 98-99 0.7080010572522646 1105 100-101 0.5630448382729547 1106 1 0.29960008389690174 1106 2 0.25476164051380223 1106 3 0.17327354716945464 1106 4 0.1465559312104574 1106 5 0.15893238203885574 1106 6 0.14334248378145276 1106 7 0.11451840694969917 1106 8 0.24992387552310902 1106 9 0.2251782978037653 1106 10-11 0.27203549155920115 1106 12-13 0.45781220610238904 1106 14-15 0.44763692058153026 1106 16-17 0.287915483843058 1106 18-19 0.4418031941547156 1106 20-21 0.47991534785187895 1106 22-23 0.3978365730210669 1106 24-25 0.37452312439999247 1106 26-27 0.5702053046158682 1106 28-29 0.589745282766863 1106 30-31 0.5130524534349021 1106 32-33 0.5156200890473883 1106 34-35 0.20329537853633184 1106 36-37 0.3351947152449313 1106 38-39 0.9136789620094135 1106 40-41 0.7471434584164811 1106 42-43 0.5961403785301513 1106 44-45 0.5653722850548704 1106 46-47 0.5723580380636406 1106 48-49 0.652326319397865 1106 50-51 0.4765705174348014 1106 52-53 0.6082643057571175 1106 54-55 0.4158812634517588 1106 56-57 0.7523602829757081 1106 58-59 0.626762148312487 1106 60-61 0.6457194428086623 1106 62-63 0.559273726359649 1106 64-65 0.7783260860559622 1106 66-67 0.6050338366996542 1106 68-69 0.720985223301696 1106 70-71 0.43333777184357203 1106 72-73 0.5638746902847451 1106 74-75 0.49244486361117623 1106 76-77 0.7955687338123703 1106 78-79 0.6463252673755449 1106 80-81 0.5358228502236138 1106 82-83 0.6425020521790543 1106 84-85 0.7490629699797289 1106 86-87 0.9841192999939494 1106 88-89 0.915614941986366 1106 90-91 0.9986419707111729 1106 92-93 1.0211203184239288 1106 94-95 1.2476542546210112 1106 96-97 1.2689575482045576 1106 98-99 1.41960071088495 1106 100-101 1.2337794082349802 1107 1 0.050244445608488064 1107 2 0.07925166483989798 1107 3 0.02633109698199121 1107 4 -0.07324136230576528 1107 5 0.1057260447455377 1107 6 0.05955735660436545 1107 7 0.1754834188494101 1107 8 0.1657420914558827 1107 9 0.22932262166012407 1107 10-11 0.24315442608283178 1107 12-13 0.2388979267835154 1107 14-15 0.35063126863231275 1107 16-17 0.4686182084315007 1107 18-19 0.2913072907480725 1107 20-21 0.19614108347759185 1107 22-23 0.34017813245870343 1107 24-25 0.33300993596986217 1107 26-27 0.30937788821830026 1107 28-29 0.2665686700521164 1107 30-31 0.16986359537882123 1107 32-33 0.19984088045195136 1107 34-35 0.15789462340695337 1107 36-37 0.30899314112605936 1107 38-39 1.0104360861975792 1107 40-41 0.710102123805946 1107 42-43 0.30426509667333335 1107 44-45 0.08802040595457328 1107 46-47 0.18019439816885097 1107 48-49 0.15098575077951892 1107 50-51 0.027947459983742817 1107 52-53 0.1826004403768735 1107 54-55 0.04904276699533483 1107 56-57 -0.07084101792722919 1107 58-59 -0.12995797651100105 1107 60-61 -0.23990676485520623 1107 62-63 -0.2116593884009319 1107 64-65 -0.18678209758466124 1107 66-67 -0.4203777153973576 1107 68-69 -0.5843229017456153 1107 70-71 -0.5850277713459455 1107 72-73 -0.6631521276711361 1107 74-75 -0.5920397622310993 1107 76-77 -0.5033155246696701 1107 78-79 -0.4369998365617249 1107 80-81 -0.5647272026091059 1107 82-83 -0.5476644778796391 1107 84-85 -0.5560797442067624 1107 86-87 -0.2911795093770273 1107 88-89 -0.5315860758253379 1107 90-91 -0.2739049286169788 1107 92-93 -0.3260532132049292 1107 94-95 -0.47624621577126547 1107 96-97 -0.24673097244629005 1107 98-99 -0.13275552979525784 1107 100-101 -0.45059609581552706 1108 1 -0.06607531163393787 1108 2 -0.03194900527215694 1108 3 0.06186442083260246 1108 4 0.03395760091709121 1108 5 8.582496354634372E-4 1108 6 0.10078505246200109 1108 7 0.04575509871285277 1108 8 0.005839763448676649 1108 9 0.1746515929432988 1108 10-11 0.346482069785516 1108 12-13 0.2420327552253525 1108 14-15 0.26757135366068496 1108 16-17 0.3893521707851093 1108 18-19 0.2356137089675343 1108 20-21 0.26644279194724163 1108 22-23 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sequence quality scores pass #Quality Count 2 3223.0 3 596.0 4 587.0 5 578.0 6 759.0 7 892.0 8 1126.0 9 1464.0 10 1914.0 11 2637.0 12 3862.0 13 5554.0 14 5972.0 15 6796.0 16 8828.0 17 11282.0 18 9791.0 19 6502.0 20 6493.0 21 6631.0 22 7236.0 23 8214.0 24 9186.0 25 10346.0 26 11612.0 27 13325.0 28 15614.0 29 18273.0 30 22013.0 31 26441.0 32 32201.0 33 41418.0 34 55481.0 35 84561.0 36 108557.0 37 135232.0 38 94823.0 39 13498.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 52.17610024735737 16.173152687878478 13.084029460427244 18.566717604336908 2 12.045132317683642 23.981057253705792 40.319744063347855 23.65406636526271 3 25.806382377003644 29.725802216004926 29.692218732008847 14.775596674982577 4 11.111377702234272 15.008757518395841 40.724291600737985 33.15557317863191 5 11.747744971494823 42.704341829736826 32.6448055408911 12.903107657877255 6 24.34320750908357 36.78996770286637 19.88330137262818 18.98352341542188 7 24.355950916374876 39.20090848869121 19.13428598466925 17.30885461026466 8 20.930473212235018 30.20529672620625 21.76097343055524 27.103256631003493 9 25.887294117350113 23.147198128173187 22.170773908006375 28.794733846470322 10-11 22.263772150205618 24.757251538511042 27.239058811059362 25.73991750022398 12-13 34.16239895329046 22.635472299922153 24.529289824736715 18.672838922050673 14-15 28.72919566454622 23.818243078682 30.677474594387235 16.77508666238455 16-17 18.376215290152594 28.331738493339838 33.61350792678581 19.678538289721768 18-19 17.229819261143795 33.41548735888058 32.95707799205971 16.397615387915913 20-21 19.497375735589266 30.16528195379578 27.88864737409463 22.44869493652032 22-23 17.926406374461756 28.77685218030173 25.90698103037135 27.38976041486516 24-25 20.82856681086186 28.633037114479876 29.57112661651401 20.96726945814425 26-27 25.081127074571263 23.52486329433048 32.17934369916736 19.214665931930895 28-29 19.413098392588207 25.431203709431248 35.8532487189813 19.302449178999247 30-31 18.371059000987717 32.921143729902525 31.76788379673896 16.939913472370797 32-33 17.6738875357167 31.580535275505365 29.217726212063926 21.527850976714006 34-35 19.863225488243273 26.610515133407315 29.795344102033912 23.730915276315496 36-37 23.40347131737596 26.608874689925276 32.18572447003972 17.801929522659037 38-39 26.51501629798411 22.61520934206779 33.54213740941169 17.32763695053641 40-41 19.54863378848403 26.648149273227112 36.48458107403413 17.318635864254723 42-43 17.2105983438347 31.947836236876455 32.08532251673319 18.756242902555652 44-45 18.364882033414634 32.82200025700644 27.653322377853897 21.15979533172504 46-47 18.06732397486825 29.694778989349075 25.034896418567108 27.203000617215565 48-49 21.05819636842505 28.249930356826297 27.604021576721454 23.087851698027198 50-51 25.003450227461784 29.79768372619501 28.01970871217253 17.17915733417067 52-53 19.114761757940926 29.85532827648121 32.15319716848358 18.87671279709428 54-55 15.731036944945927 28.83306029538739 33.85586128490729 21.580041474759394 56-57 20.406188698348416 33.1955957729545 26.08409795608429 20.314117572612794 58-59 18.656617259047696 29.566176386844827 28.27389275766898 23.503313596438502 60-61 19.16214472735552 25.8997078131522 31.00462945395211 23.933518005540165 62-63 26.922947869032836 24.94617107121626 28.09879578940971 20.03208527034119 64-65 23.787757830755528 26.75279964901907 31.776878941334147 17.68256357889126 66-67 18.619042614215545 27.7453327981178 33.38428011188875 20.25134447577791 68-69 17.58051167553062 33.18646971998609 30.544761002581616 18.688257601901668 70-71 18.410168740304556 31.76408015955931 26.300819957577488 23.524931142558646 72-73 18.14350265817183 29.050332738407654 25.500088953945916 27.306075649474604 74-75 19.97525648915667 29.625099088010415 28.190064100128907 22.209580322704007 76-77 23.127491313438487 25.159156037801182 30.334188115884963 21.379164532875368 78-79 19.360200207220043 25.47977389343928 34.00934072506761 21.150685174273068 80-81 18.136265505645927 32.28009815258208 30.54141516175113 19.042221180020874 82-83 17.639105425970904 30.722544944236297 28.004210683565105 23.634138946227697 84-85 19.12404415860637 26.671139919987848 28.245999392312754 25.958816529093028 86-87 21.963542955746284 26.70224974790746 30.129758820390474 21.204448475955783 88-89 24.46660821508494 22.642095900687174 31.655899547686044 21.23539633654184 90-91 19.38339212211351 25.18280872973813 34.3606332346239 21.07316591352446 92-93 17.371152428785265 29.9235487947886 31.861549488763437 20.843749287662696 94-95 17.489611225891174 31.188609990572065 28.502186660453138 22.819592123083627 96-97 17.027627170907543 27.812690149308168 26.398311711582796 28.761370968201494 98-99 19.91284186426985 26.564680377959615 27.628040024616507 25.894437733154028 100-101 23.33985784498672 26.45617731141165 29.038402617232308 21.165562226369325 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3754.0 1 2373.0 2 1553.0 3 2021.5 4 1546.0 5 1448.5 6 2657.0 7 5020.0 8 6831.5 9 6431.0 10 4632.5 11 3322.0 12 3052.0 13 2957.5 14 2708.0 15 2464.0 16 2227.0 17 2055.5 18 2037.5 19 2028.0 20 2028.5 21 2227.0 22 2429.5 23 2671.0 24 3010.5 25 3488.5 26 4269.0 27 5227.0 28 6079.0 29 7003.5 30 8168.5 31 9156.0 32 10225.5 33 11390.0 34 12579.5 35 13485.5 36 14117.0 37 14757.5 38 14773.0 39 14494.5 40 14235.5 41 14026.5 42 14028.5 43 14038.0 44 15814.0 45 17551.0 46 24094.0 47 43379.0 48 137796.5 49 139952.0 50 48399.5 51 26871.0 52 13585.0 53 8595.5 54 6552.0 55 5294.0 56 4575.0 57 3962.5 58 3536.0 59 3214.5 60 3002.0 61 2918.5 62 2425.5 63 1377.5 64 603.5 65 446.0 66 368.0 67 301.5 68 257.0 69 224.0 70 185.0 71 154.5 72 143.0 73 185.0 74 171.0 75 113.0 76 82.0 77 53.5 78 47.5 79 39.5 80 27.5 81 19.5 82 15.0 83 12.5 84 8.5 85 4.0 86 3.5 87 3.0 88 1.5 89 0.5 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 1.0 96 1.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2970309507006755 2 0.1826040931545519 3 0.1844944046778909 4 0.20528783143462043 5 0.21852001209799374 6 0.11089827603589072 7 0.12539066438149007 8 0.13937896965419902 9 0.1435376550055449 10-11 0.130179453573949 12-13 0.11858554289746949 14-15 0.13572436737574353 16-17 0.10831485028732736 18-19 0.13937896965419902 20-21 0.1635749571529388 22-23 0.1712622240145176 24-25 0.19381994152636353 26-27 0.17624004435931043 28-29 0.17353059784252445 30-31 0.16225173908660145 32-33 0.16993900594818026 34-35 0.35317320294384513 36-37 0.4777447323318883 38-39 0.4467436233491279 40-41 0.43502369190442586 42-43 0.456573243270491 44-45 0.4607949390059482 46-47 0.46407147897973583 48-49 0.4763585038814397 50-51 0.4684191954834157 52-53 0.4746572235104345 54-55 0.45896763786672046 56-57 0.4246899889101724 58-59 0.41473434822058675 60-61 0.3692408508922271 62-63 0.47251487045065027 64-65 0.47049853815908865 66-67 0.47976106462345 68-69 0.4912919649158181 70-71 0.48454985381590887 72-73 0.47705161810666397 74-75 0.4811472930738986 76-77 0.4600388143966126 78-79 0.44592448835568105 80-81 0.4442862183687872 82-83 0.4567622744228249 84-85 0.45871559633027525 86-87 0.4576444198003831 88-89 0.4653316866619619 90-91 0.4824705111402359 92-93 0.48719628994858355 94-95 0.4846128642000202 96-97 0.48448684343179754 98-99 0.4796350438552273 100-101 0.46350438552273415 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 793520.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 32.3032059862573 #Duplication Level Percentage of deduplicated Percentage of total 1 77.9802711857992 25.19012762979077 2 12.160128841842631 7.856222935949421 3 4.1274062448641455 3.9998536235043383 4 1.903421447272462 2.459464603596093 5 1.0231931140573993 1.652620896355812 6 0.6464172492885165 1.2528809734102073 7 0.4099142314455994 0.9269080698559884 8 0.2876398038805198 0.7433350267679267 9 0.20724197018899726 0.6025122046811677 >10 1.0639640099740526 6.209976122521662 >50 0.08327639631848059 1.8110777620295024 >100 0.07272023336734286 5.007869971477037 >500 0.010947131904761289 2.5361403434185004 >1k 0.016420697857141935 10.909028158132553 >5k 0.004300658962584792 9.374790177362277 >10k+ 0.0027367829761903223 19.46719150114674 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 40654 5.1232483113217056 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 33649 4.240472829922371 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 25073 3.1597187216453273 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 15434 1.9450045367476558 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 14573 1.8365006553079946 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 13787 1.7374483314850289 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 10970 1.3824478274019558 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 9755 1.2293325940114932 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9184 1.1573747353563868 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 7847 0.9888849682427665 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 6442 0.8118257888900091 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 6259 0.7887639883052726 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6168 0.7772960983970159 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 6146 0.7745236414961186 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 6009 0.7572587962496219 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5607 0.7065984474241356 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5488 0.6916019760056458 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 5324 0.6709345700171389 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 4824 0.6079241859058373 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4114 0.5184494404677891 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 4075 0.5135346305071076 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 3820 0.48139933461034373 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3617 0.4558171186611553 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 3306 0.4166246597439258 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 3256 0.41032362133279565 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 3228 0.40679503982256277 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2809 0.35399233793729207 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 2735 0.3446668010888194 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 2601 0.32778001814699065 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2384 0.30043351144268576 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 2269 0.2859411230970864 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 2261 0.2849329569513056 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2219 0.27964008468595625 No Hit GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT 2097 0.2642655509627987 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2044 0.2575864502470007 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 1965 0.24763080955741507 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 1950 0.24574049803407602 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1808 0.22784554894646636 No Hit AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG 1663 0.20957253755418895 No Hit CTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACT 1612 0.20314547837483615 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1495 0.18840104849279163 No Hit TACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGA 1469 0.18512450851900394 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 1440 0.18146990624054843 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 1436 0.18096582316765802 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1417 0.17857142857142858 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1399 0.17630305474342173 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1378 0.17365661861074705 No Hit GCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT 1374 0.17315253553785664 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1338 0.16861578788184292 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1332 0.16785966327250731 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1302 0.16407904022582923 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 1278 0.16105454178848674 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 1228 0.15475350337735658 No Hit GCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAA 1207 0.15210706724468193 No Hit TCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTA 1153 0.14530194576066136 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1152 0.14517592499243875 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1117 0.14076519810464766 No Hit GTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA 1103 0.13900090734953122 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 1075 0.13547232583929833 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1027 0.12942332896461337 No Hit GTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 999 0.12589474745438048 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 988 0.12450851900393184 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 987 0.12438249823570925 No Hit CATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 968 0.12198810363947979 No Hit GAGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA 887 0.11178042141344895 No Hit GAGTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 866 0.10913398528077428 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 854 0.10762173606210304 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 850 0.10711765298921261 No Hit TTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCG 847 0.10673959068454482 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 807 0.10169875995564069 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.260207682226031E-4 0.0 12-13 0.0 0.0 0.0 1.8903115233390465E-4 0.0 14-15 0.0 0.0 0.0 2.520415364452062E-4 0.0 16-17 0.0 0.0 0.0 3.150519205565077E-4 0.0 18-19 0.0 0.0 0.0 3.7806230466780924E-4 0.0 20-21 0.0 0.0 0.0 8.1913499344692E-4 0.0 22-23 0.0 0.0 0.0 0.002835467285008569 0.0 24-25 0.0 0.0 0.0 0.005355882649460631 0.0 26-27 0.0 0.0 0.0 0.008758443391470914 0.0 28-29 0.0 0.0 0.0 0.017705917935275733 0.0 30-31 0.0 0.0 0.0 0.04190190543401553 0.0 32-33 0.0 0.0 0.0 0.08052727089424336 0.0 34-35 0.0 0.0 0.0 0.15834509527170076 0.0 36-37 0.0 0.0 0.0 0.26445458211513256 0.0 38-39 0.0 0.0 0.0 0.3841113015424942 0.0 40-41 0.0 0.0 0.0 0.5151098901098902 0.0 42-43 0.0 0.0 0.0 0.649448029035185 0.0 44-45 0.0 0.0 0.0 0.7871257183183789 0.0 46-47 0.0 0.0 0.0 0.9760308498840609 0.0 48-49 0.0 0.0 0.0 1.2038763988305274 0.0 50-51 0.0 0.0 0.0 1.4225224316967435 0.0 52-53 0.0 0.0 0.0 1.608970158282085 0.0 54-55 0.0 0.0 0.0 1.8034832140336727 0.0 56-57 0.0 0.0 0.0 2.02168817421111 0.0 58-59 0.0 0.0 0.0 2.3671741102933765 0.0 60-61 0.0 0.0 0.0 2.906416977517895 0.0 62-63 0.0 0.0 0.0 3.3202691803609232 0.0 64-65 0.0 0.0 0.0 3.624546325234399 0.0 66-67 0.0 0.0 0.0 3.9626600463756425 0.0 68-69 0.0 0.0 0.0 4.338516987599556 0.0 70-71 0.0 0.0 0.0 4.80296652888396 0.0 72-73 0.0 0.0 0.0 5.363191854017542 0.0 74-75 0.0 0.0 0.0 5.897141848976712 0.0 76-77 0.0 0.0 0.0 6.349997479584635 0.0 78-79 0.0 0.0 0.0 6.856979030144168 0.0 80-81 0.0 0.0 0.0 7.402459925395705 0.0 82-83 0.0 0.0 0.0 7.9722628289142055 0.0 84-85 0.0 0.0 0.0 8.959509527170077 0.0 86-87 0.0 0.0 0.0 10.045934570017138 0.0 88-89 0.0 0.0 0.0 10.755620526262728 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCCGTAC 25 0.005060692 56.88586 3 GTAAGCG 35 7.8594894E-8 47.54899 94-95 GCTTATG 165 1.8189894E-12 34.517685 1 ACTAACG 30 0.003926635 31.707355 34-35 CCGGGTC 255 0.0 31.585308 9 CATTCCG 80 4.5605862E-4 29.611223 9 AGTACTC 415 0.0 28.557156 5 AAGTACT 390 0.0 27.956724 4 GTATAGC 170 3.6689016E-9 27.918715 1 CTCCCCG 290 0.0 27.789066 5 TCCCCGG 290 0.0 27.775038 6 CTACCTC 320 0.0 26.697273 1 GACTAGG 110 9.5248935E-5 25.888264 1 CGCCGTA 65 9.94433E-6 25.603302 94-95 GCCGTAT 65 9.94433E-6 25.603302 94-95 CCCGGGT 315 0.0 25.570671 8 TACCTCC 320 0.0 25.183842 2 AAAGTAC 405 0.0 24.580307 3 CTTATGT 215 1.6989361E-9 24.253658 2 ATGGGGA 680 0.0 23.70244 5 >>END_MODULE