##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939291_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 793520 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.11267390866015 33.0 31.0 34.0 30.0 34.0 2 32.278166901905436 33.0 31.0 34.0 30.0 34.0 3 32.37149410222805 34.0 31.0 34.0 30.0 34.0 4 35.84774044762577 37.0 35.0 37.0 35.0 37.0 5 35.815646738582515 37.0 35.0 37.0 35.0 37.0 6 35.921092095977414 37.0 35.0 37.0 35.0 37.0 7 35.88495312027422 37.0 35.0 37.0 35.0 37.0 8 35.91290074604295 37.0 35.0 37.0 35.0 37.0 9 37.507052122189734 39.0 37.0 39.0 35.0 39.0 10-11 37.42110028732735 39.0 37.0 39.0 34.5 39.0 12-13 37.32950398225627 39.0 37.0 39.0 34.0 39.0 14-15 38.226072436737574 40.0 37.5 41.0 33.0 41.0 16-17 38.281001108982764 40.0 37.5 41.0 33.5 41.0 18-19 38.692171589878015 40.0 38.0 41.0 34.0 41.0 20-21 38.69906366569211 40.0 38.0 41.0 34.0 41.0 22-23 38.689455212218974 40.0 38.0 41.0 34.0 41.0 24-25 38.575844339147096 40.0 38.0 41.0 34.0 41.0 26-27 38.10351408912189 40.0 37.5 41.0 33.5 41.0 28-29 37.83643512450852 40.0 37.0 41.0 33.0 41.0 30-31 37.722027170077624 39.5 37.0 41.0 33.0 41.0 32-33 37.629551870148205 40.0 36.0 41.0 33.0 41.0 34-35 37.55413159088618 40.0 36.0 41.0 33.0 41.0 36-37 37.20677046577276 40.0 35.0 41.0 32.5 41.0 38-39 36.84775367980643 39.0 35.0 41.0 31.5 41.0 40-41 36.526349682427664 39.0 35.0 41.0 30.5 41.0 42-43 36.486616594414755 39.0 35.0 41.0 31.0 41.0 44-45 36.2687959975804 39.0 35.0 41.0 30.0 41.0 46-47 35.760053306784954 39.0 35.0 41.0 28.5 41.0 48-49 35.36499836173002 39.0 35.0 41.0 27.0 41.0 50-51 35.13768525052929 39.0 35.0 40.0 26.0 41.0 52-53 34.550681772356086 38.0 34.0 40.0 23.5 41.0 54-55 34.38906139731828 38.0 34.0 40.0 23.0 41.0 56-57 34.32825511644319 38.0 34.0 40.0 23.0 41.0 58-59 34.16634867426152 38.0 34.0 40.0 22.5 41.0 60-61 33.722608125819136 37.5 33.5 40.0 21.5 41.0 62-63 33.164925269684446 37.0 32.5 39.0 20.5 41.0 64-65 32.57793817421111 36.0 32.0 39.0 18.0 40.5 66-67 32.15669107268878 36.0 31.5 39.0 13.0 40.0 68-69 31.8405484423833 35.0 31.0 38.0 12.5 40.0 70-71 31.268474014517594 35.0 31.0 37.5 7.5 39.0 72-73 30.626776262728097 35.0 30.5 37.0 4.5 39.0 74-75 30.0926970964815 34.0 30.0 36.0 2.0 38.5 76-77 27.9500321352959 31.5 26.0 34.5 2.0 36.0 78-79 28.759534731323722 33.5 29.0 35.0 2.0 37.0 80-81 28.935849758040124 34.0 29.0 35.0 2.0 36.5 82-83 28.64972590482912 34.0 29.0 35.0 2.0 36.0 84-85 28.28832669623954 34.0 29.0 35.0 2.0 36.0 86-87 27.555379196491582 33.0 26.5 35.0 2.0 35.0 88-89 26.693973686863593 32.5 25.0 35.0 2.0 35.0 90-91 25.966273691904426 32.0 23.5 34.0 2.0 35.0 92-93 25.763084736364554 32.0 23.5 34.0 2.0 35.0 94-95 25.33058713075915 32.0 21.5 34.0 2.0 35.0 96-97 24.369229509023086 31.0 11.5 34.0 2.0 35.0 98-99 23.30760850388144 31.0 2.0 34.0 2.0 35.0 100-101 19.37235356386733 25.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.223364190460849 1101 2 0.25024952392786304 1101 3 0.2994550230854074 1101 4 0.2265292642180654 1101 5 0.15061433824712367 1101 6 0.20717979523158903 1101 7 0.28365489666737886 1101 8 0.27231643019122487 1101 9 0.46020345803739104 1101 10-11 0.3957988199304623 1101 12-13 0.42842530978000326 1101 14-15 0.42074393600186255 1101 16-17 0.3941131602569712 1101 18-19 0.39372891897507145 1101 20-21 0.43177089168565885 1101 22-23 0.44268709871331424 1101 24-25 0.5698409203176951 1101 26-27 0.5592136238108623 1101 28-29 0.5358267342365366 1101 30-31 0.5768260808044658 1101 32-33 0.6534000072736177 1101 34-35 0.8808521380719867 1101 36-37 0.8793344069012576 1101 38-39 0.8195024360196328 1101 40-41 0.9177451037425612 1101 42-43 0.8756295522857371 1101 44-45 0.9345046961468171 1101 46-47 1.0574512405700531 1101 48-49 1.1681445512783455 1101 50-51 0.9570229364479985 1101 52-53 0.8526993999226562 1101 54-55 0.6368539693784001 1101 56-57 0.8707680004559464 1101 58-59 0.9629180511564925 1101 60-61 0.9443101494500539 1101 62-63 0.6313730695977213 1101 64-65 0.9166968310026746 1101 66-67 0.8781942387830881 1101 68-69 0.9687781595828504 1101 70-71 0.7902473216277706 1101 72-73 1.0587076427261763 1101 74-75 1.2493539502158768 1101 76-77 1.0491178480145713 1101 78-79 0.9789860015659109 1101 80-81 1.3103897109434328 1101 82-83 1.2522457220145782 1101 84-85 1.284909731818825 1101 86-87 1.1667713990273683 1101 88-89 1.4744574071033902 1101 90-91 1.6902622679834103 1101 92-93 1.4633587672179473 1101 94-95 1.2058374170617263 1101 96-97 1.54434800367261 1101 98-99 1.3588351976510324 1101 100-101 1.5321403279791816 1102 1 0.187785231186254 1102 2 0.2277450886019281 1102 3 0.19561707847933008 1102 4 0.08518951301524424 1102 5 0.18392065117691203 1102 6 0.17392794202988426 1102 7 0.28028886655114604 1102 8 0.21095497888136094 1102 9 0.31069474762280436 1102 10-11 0.27840773415010744 1102 12-13 0.3530027756012899 1102 14-15 0.35470523189204073 1102 16-17 0.2809259465632863 1102 18-19 0.24615248160480974 1102 20-21 0.1940437055988653 1102 22-23 0.18003620861095015 1102 24-25 0.4201457900646872 1102 26-27 0.35601599993069044 1102 28-29 0.41881477194780103 1102 30-31 0.42504472404507965 1102 32-33 0.4585944180298398 1102 34-35 0.6301342122335711 1102 36-37 0.6837600976496034 1102 38-39 0.6869181334078647 1102 40-41 0.6191581242256987 1102 42-43 0.7147104815362653 1102 44-45 0.9178431612622191 1102 46-47 0.9457208246024678 1102 48-49 0.7601490253544725 1102 50-51 0.6605913054000965 1102 52-53 0.4462926318352203 1102 54-55 0.48905445658351 1102 56-57 0.6250153355948314 1102 58-59 0.6137777817484036 1102 60-61 0.7120593079425248 1102 62-63 0.5420800615984263 1102 64-65 0.6247706951507936 1102 66-67 0.5650937417333424 1102 68-69 0.48796474070584495 1102 70-71 0.4121994096346917 1102 72-73 0.43299154966706865 1102 74-75 0.38512833613008013 1102 76-77 0.5109499570513272 1102 78-79 0.4269675355288811 1102 80-81 0.6019272975815291 1102 82-83 0.7080547945863565 1102 84-85 0.32448519729240743 1102 86-87 0.48079666415198474 1102 88-89 0.43606434611059086 1102 90-91 0.5221429928005001 1102 92-93 0.2928244125552517 1102 94-95 -0.15327107718963262 1102 96-97 -0.4959312490561345 1102 98-99 -0.28686971021777197 1102 100-101 -0.15811682666759452 1103 1 0.07511822247239053 1103 2 0.19381358980952257 1103 3 0.29649597291605545 1103 4 0.2004969592158048 1103 5 0.1449835836893314 1103 6 0.16098437564561152 1103 7 0.13478532719695124 1103 8 0.18032298412052228 1103 9 0.1553907218941788 1103 10-11 0.18924917024776278 1103 12-13 0.27907886858806563 1103 14-15 0.286067413986288 1103 16-17 0.3077902080541932 1103 18-19 0.33130763337781843 1103 20-21 0.42893105944668264 1103 22-23 0.3365770269380022 1103 24-25 0.3411839867251345 1103 26-27 0.3924002273837317 1103 28-29 0.4813624812744308 1103 30-31 0.4379371919155801 1103 32-33 0.5265888117928981 1103 34-35 0.6579818594077125 1103 36-37 0.7085929325117135 1103 38-39 0.490234739799142 1103 40-41 0.5368071337487947 1103 42-43 0.38526480655618656 1103 44-45 0.46331875285786595 1103 46-47 0.7539376741809534 1103 48-49 0.5609492906496101 1103 50-51 0.7000685571760243 1103 52-53 0.6499952927121129 1103 54-55 0.8338661846309563 1103 56-57 0.7351774743074841 1103 58-59 0.796419512340357 1103 60-61 0.8851383166730642 1103 62-63 1.0274700579693814 1103 64-65 0.78133862697495 1103 66-67 0.5575071766797137 1103 68-69 0.4538820320257706 1103 70-71 0.6175372229520022 1103 72-73 0.701032453505924 1103 74-75 0.44938940990799026 1103 76-77 0.5085378986363374 1103 78-79 0.8304893702366591 1103 80-81 1.0614259898767067 1103 82-83 0.8046893839002998 1103 84-85 0.6357742146575589 1103 86-87 0.6159845606868792 1103 88-89 0.7024815686039467 1103 90-91 0.8741543971545092 1103 92-93 0.9510212398589601 1103 94-95 0.8133261372113907 1103 96-97 0.7762329919953679 1103 98-99 0.4009068995939771 1103 100-101 0.5988993121201425 1104 1 0.10130079410950543 1104 2 0.1351500311459617 1104 3 0.06846979034441603 1104 4 0.02753187715981653 1104 5 0.10436763398247706 1104 6 0.12610174985389477 1104 7 0.07489740367266506 1104 8 0.08056079344924427 1104 9 0.22906452284070866 1104 10-11 -0.023596065324738902 1104 12-13 -0.06340776935311254 1104 14-15 0.18674081465968584 1104 16-17 0.14908779935178273 1104 18-19 -0.07304474824728402 1104 20-21 0.051685409473762434 1104 22-23 0.03717272753370793 1104 24-25 0.09648760566512493 1104 26-27 0.18190365325079938 1104 28-29 0.27328520501533404 1104 30-31 0.25175721028105613 1104 32-33 0.06566841905431886 1104 34-35 0.038435580770531885 1104 36-37 -0.0025452558992000718 1104 38-39 -0.007375394174623295 1104 40-41 0.19905902327341352 1104 42-43 0.3072762967494924 1104 44-45 0.2961399720427238 1104 46-47 0.11310039698004459 1104 48-49 0.15056926663321946 1104 50-51 0.29595163033182814 1104 52-53 0.27806503441821917 1104 54-55 0.5001090326919879 1104 56-57 0.39740581835400945 1104 58-59 0.44096520234059255 1104 60-61 0.6545751088371361 1104 62-63 0.4812914890635511 1104 64-65 0.4694611105519755 1104 66-67 0.7568431941975504 1104 68-69 0.7179327960765356 1104 70-71 0.5813183912786286 1104 72-73 0.5025205277212699 1104 74-75 0.4674311324951681 1104 76-77 0.5458339359323752 1104 78-79 0.7135566396675657 1104 80-81 1.0067357306409903 1104 82-83 0.6025749999677323 1104 84-85 0.7625430686991415 1104 86-87 0.7702545331386723 1104 88-89 0.8073178098038234 1104 90-91 0.9586237998057321 1104 92-93 0.7946969244437341 1104 94-95 0.860264047785666 1104 96-97 0.8116336183050876 1104 98-99 0.8916299812261492 1104 100-101 1.0201338015364492 1105 1 0.044560369333893846 1105 2 0.073810832709988 1105 3 0.1661770684036057 1105 4 0.12018494745218078 1105 5 0.10183497602459823 1105 6 0.14476344006983766 1105 7 0.105433705103394 1105 8 0.17864574857232185 1105 9 0.3047221252578396 1105 10-11 0.2755463855772433 1105 12-13 0.31401936118984963 1105 14-15 0.3499861714534305 1105 16-17 0.4028756781396581 1105 18-19 0.30596529351934976 1105 20-21 0.3069006854001799 1105 22-23 0.17408621726977458 1105 24-25 0.14677974998219412 1105 26-27 0.17407240824945802 1105 28-29 0.27976680992069447 1105 30-31 0.16351962919156904 1105 32-33 0.013182415321985275 1105 34-35 0.030954242460751402 1105 36-37 -0.023767374445363032 1105 38-39 -0.11220299570310743 1105 40-41 0.18887840477167828 1105 42-43 0.07718765372096215 1105 44-45 -0.032011409811005365 1105 46-47 0.029900361433966793 1105 48-49 -0.0969980487493487 1105 50-51 -0.17836632932337437 1105 52-53 0.020072721257285764 1105 54-55 -0.08016400560890702 1105 56-57 -0.023409741941030404 1105 58-59 0.03182986065392868 1105 60-61 -0.07461377412741399 1105 62-63 -0.06432412455060188 1105 64-65 -0.07701538460487001 1105 66-67 -0.05841162673908684 1105 68-69 0.07918646176569055 1105 70-71 0.011164876660302525 1105 72-73 0.03811659099328679 1105 74-75 0.12015151817009695 1105 76-77 0.3047537795296371 1105 78-79 0.27580115224199275 1105 80-81 0.3008628522974419 1105 82-83 0.3325303452090047 1105 84-85 0.5921718343616291 1105 86-87 0.5030813348804308 1105 88-89 0.48332087565293236 1105 90-91 0.21766027896684292 1105 92-93 0.39358166629035196 1105 94-95 0.7379022331584544 1105 96-97 0.5688761314127007 1105 98-99 0.4731026996123582 1105 100-101 0.3903017567483893 1106 1 0.124579565594793 1106 2 0.14040307908982896 1106 3 0.21247065991273217 1106 4 0.0832234512566572 1106 5 0.13789056171921033 1106 6 0.1184520433332068 1106 7 0.09673105240270274 1106 8 0.040373551156861254 1106 9 0.1348856412338577 1106 10-11 0.10457794812505483 1106 12-13 0.15708036495954758 1106 14-15 0.14856801290344634 1106 16-17 0.10139874842858632 1106 18-19 0.21276257822215427 1106 20-21 0.20062660313446656 1106 22-23 0.34298636193724974 1106 24-25 0.15941815780536928 1106 26-27 0.16286937301458693 1106 28-29 0.27070857430745576 1106 30-31 0.40131939688899365 1106 32-33 0.33167168349930876 1106 34-35 0.58086712899415 1106 36-37 0.5243632505459388 1106 38-39 0.3687485406932822 1106 40-41 0.5691182537054544 1106 42-43 0.5748672361327394 1106 44-45 0.6866964244890994 1106 46-47 0.5641684692112321 1106 48-49 0.8166657245678479 1106 50-51 0.8187020677888341 1106 52-53 0.9729784161480168 1106 54-55 0.6305540806073395 1106 56-57 0.7415499528662437 1106 58-59 0.92356225364869 1106 60-61 1.0060357341422872 1106 62-63 0.8698147909997616 1106 64-65 0.9237740268488963 1106 66-67 0.9370336082760033 1106 68-69 1.0378492961353452 1106 70-71 0.8012675001492902 1106 72-73 0.7385219033498345 1106 74-75 0.5112953301580241 1106 76-77 0.5896322032105878 1106 78-79 0.757294322268006 1106 80-81 0.5865141689105542 1106 82-83 0.7771889508371252 1106 84-85 0.8175566289755487 1106 86-87 0.8581147892336709 1106 88-89 0.9136118450641035 1106 90-91 1.0550006578843387 1106 92-93 1.1427852996695904 1106 94-95 1.3208973714967236 1106 96-97 1.1982770133278748 1106 98-99 1.5457868028826773 1106 100-101 1.2158383478819452 1107 1 0.0821537371414891 1107 2 0.032790558557486804 1107 3 0.08540878472962987 1107 4 0.04845589877779588 1107 5 0.025129731011055867 1107 6 0.05266789710012887 1107 7 -0.04419077162647511 1107 8 -0.02641458139567021 1107 9 0.03478655930528163 1107 10-11 0.010381811991095447 1107 12-13 0.03860997926421561 1107 14-15 0.11699758079431177 1107 16-17 -0.009019996160596122 1107 18-19 0.1363847304396515 1107 20-21 0.05859874827468303 1107 22-23 0.10211605392741774 1107 24-25 -0.029392990722065804 1107 26-27 0.050960284798748035 1107 28-29 0.04946126120527339 1107 30-31 0.10354363805637945 1107 32-33 -0.1584604476838436 1107 34-35 0.009105149462307338 1107 36-37 -0.13841996411949964 1107 38-39 0.013227932639672701 1107 40-41 0.03462144835700087 1107 42-43 0.13536811009460337 1107 44-45 0.21070897925573462 1107 46-47 -0.14538487208815098 1107 48-49 -0.407276715744338 1107 50-51 -0.31597611344879795 1107 52-53 -0.22700825223036247 1107 54-55 -0.0784454223371327 1107 56-57 -0.06733878240725488 1107 58-59 -0.13641687660290103 1107 60-61 -0.12072823690340329 1107 62-63 -0.11195709672494303 1107 64-65 -0.048471511995494154 1107 66-67 -0.07670043081032674 1107 68-69 -0.17785716459891532 1107 70-71 -0.048216022953912585 1107 72-73 -0.055225566110873814 1107 74-75 -0.3901856319571273 1107 76-77 -0.1584223675605685 1107 78-79 -0.10065066586520643 1107 80-81 0.0026245284034871474 1107 82-83 -0.07008497942367953 1107 84-85 -0.007839451856870738 1107 86-87 0.21140440801512383 1107 88-89 0.659083208411694 1107 90-91 0.7420128604328191 1107 92-93 0.6616190954949843 1107 94-95 0.7405134537116815 1107 96-97 0.5840612253961197 1107 98-99 0.3295676507738108 1107 100-101 0.053233711111026594 1108 1 0.03111616977468401 1108 2 0.0314045013134745 1108 3 -4.161326917042629E-4 1108 4 -0.053849982087655235 1108 5 -0.10321411233444877 1108 6 0.02560896200932916 1108 7 -0.05410179212495336 1108 8 0.08166785503060936 1108 9 0.09965467050595578 1108 10-11 -0.028744671659119092 1108 12-13 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103.0 7 219.0 8 386.0 9 692.0 10 1200.0 11 1853.0 12 2689.0 13 3882.0 14 4603.0 15 6120.0 16 8216.0 17 11454.0 18 10350.0 19 6511.0 20 5793.0 21 6248.0 22 6695.0 23 7454.0 24 8481.0 25 9713.0 26 11053.0 27 13261.0 28 16080.0 29 18889.0 30 22699.0 31 27224.0 32 33262.0 33 42220.0 34 57332.0 35 85681.0 36 116536.0 37 148165.0 38 92135.0 39 6274.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 51.18235130557872 16.73428376431334 13.760900887237431 18.322464042870504 2 12.636606512753302 24.72640891218873 39.64991430587761 22.98707026918036 3 25.711891319689485 29.609713680814597 30.050660348825488 14.62773465067043 4 11.422900494001412 15.73684343179756 39.99533723157576 32.84491884262526 5 11.319941526363545 42.97081359007964 32.82084887589475 12.888396007662061 6 24.338895049904224 37.62702893436838 18.792216957354572 19.24185905837282 7 24.019432402459927 38.996244581106964 19.149233793729206 17.8350892227039 8 20.86273817925194 31.115787881842927 21.3314094162718 26.690064522633328 9 25.520087710454682 23.23596128642 21.641420506099404 29.60253049702591 10-11 22.45885421917532 25.037932251235002 26.940530799475752 25.562682730113924 12-13 34.180486944248415 22.857016836374637 24.587344994455083 18.37515122492187 14-15 28.488496650317323 24.192824773033163 30.57480201837915 16.743876558270365 16-17 18.066021477868393 28.625437846441372 33.592454832268345 19.71608584342189 18-19 17.137722686994692 33.263559702766855 33.03533566010118 16.56338195013727 20-21 19.464915818126826 29.892189232785565 28.2760989010989 22.366796047988707 22-23 18.060666397822363 28.427764895654807 26.379549349732834 27.13201935679 24-25 20.97552676681117 28.58346355479383 29.861818227643916 20.579191450751082 26-27 24.907658165715 23.499500327024307 32.78138267640725 18.81145883085345 28-29 19.43637211412441 25.2843658634943 36.03110192559733 19.248160096783952 30-31 18.29096935174917 32.761178042141346 32.018663675773766 16.92918893033572 32-33 17.777182679705618 31.220763181772355 29.722313237221492 21.279740901300535 34-35 19.906428105594465 26.285195792100264 30.232606497103053 23.57576960520222 36-37 23.351212448501972 26.439770781005624 32.45840774212737 17.75060902836503 38-39 26.359214644477223 22.352285852371864 34.19046364810104 17.09803585504987 40-41 19.57960482248392 26.43979173059825 36.7094039444657 17.271199502452138 42-43 17.14776296679027 31.658659071791106 32.534317138563424 18.6592608228552 44-45 18.425322476066732 32.41395649826057 27.99636046746821 21.164360558204482 46-47 18.295002016332294 29.48495312027422 25.331497630809558 26.88854723258393 48-49 21.290884390776267 27.86149778580952 27.927154771536372 22.92046305187784 50-51 24.94886700885549 29.496962265553815 28.315705105989903 17.23846561960079 52-53 19.251926232297187 29.654084721455593 32.36404215153279 18.729946894714423 54-55 15.65209446516786 28.678924286722452 33.87293325940115 21.79604798870854 56-57 20.495720291564588 32.91676748429765 26.006475012350162 20.5810372117876 58-59 18.843572307133925 29.373575936605235 28.19097320240352 23.59187855385732 60-61 19.303011227912318 25.924952757577536 30.895756656944084 23.876279357566066 62-63 26.97026750799298 24.89996962857384 28.246623541761974 19.88313932167121 64-65 23.756646028639516 26.76249718028176 31.683236318223003 17.797620472855723 66-67 18.468511327125935 27.86397997266594 33.19204224744192 20.475466452766202 68-69 17.717824439890816 32.99578075024007 30.3139484767601 18.972446333109012 70-71 18.652018852706924 31.632977114628492 26.188186813186814 23.52681721947777 72-73 18.41554088113721 28.822335920959773 25.255948180260106 27.50617501764291 74-75 20.22463201935679 29.693265450146182 27.718141949793324 22.3639605807037 76-77 23.14335365934547 25.19568498113427 30.249224745724813 21.41173661379545 78-79 19.395275217449385 25.382158942834316 33.64725185818091 21.57531398153539 80-81 18.128362395859458 32.19866190979674 30.117930383519486 19.555045310824315 82-83 17.871131162415566 30.790717310212724 27.61033118257889 23.72782034479282 84-85 19.288675773767515 26.70222552676681 27.601824780723867 26.407273918741808 86-87 21.899508644451593 26.85967667669562 29.47042090058723 21.77039377826556 88-89 24.362189885345323 22.875979434252216 31.19770817128005 21.564122509122413 90-91 19.49452599017408 25.32986848223853 33.5912385515861 21.58436697600129 92-93 17.309141546526867 29.94681923581006 31.184280169371913 21.55975904829116 94-95 17.691866619618914 31.231726988607722 27.487208892025407 23.589197499747957 96-97 17.315946668010888 28.143146990624057 25.438678294182882 29.102228047182177 98-99 20.2686260388056 26.73129015733073 26.75517110795639 26.244912695907285 100-101 23.25715329892945 26.919528931400162 28.041309868118436 21.782007901551957 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2768.0 1 1846.0 2 1482.0 3 1920.0 4 1447.0 5 1326.0 6 2435.0 7 4853.0 8 6685.5 9 6418.0 10 4725.0 11 3241.5 12 2862.5 13 2708.5 14 2470.5 15 2202.0 16 1963.5 17 1828.0 18 1793.5 19 1842.0 20 1942.5 21 2113.5 22 2322.5 23 2596.5 24 2881.5 25 3297.0 26 3994.0 27 4916.5 28 5884.0 29 6834.0 30 7931.5 31 9136.0 32 10339.5 33 11468.5 34 12534.0 35 13539.0 36 14428.0 37 14903.5 38 14906.0 39 14802.5 40 14545.0 41 14300.5 42 14164.0 43 14051.5 44 15849.0 45 17399.5 46 23159.5 47 42344.0 48 137707.0 49 137761.5 50 45542.5 51 28997.5 52 18901.0 53 12059.0 54 7391.5 55 5376.0 56 4604.0 57 4045.0 58 3705.5 59 3406.5 60 3010.5 61 2798.0 62 2239.0 63 1200.0 64 510.0 65 378.5 66 298.0 67 242.0 68 217.0 69 170.5 70 142.5 71 123.5 72 98.0 73 144.0 74 138.5 75 87.0 76 66.5 77 44.5 78 36.0 79 23.0 80 13.0 81 11.0 82 7.5 83 4.0 84 2.0 85 1.0 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2898477669119871 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.001512249218671237 16-17 6.931142252243169E-4 18-19 1.8903115233390462E-4 20-21 0.0 22-23 0.0 24-25 0.0 26-27 1.260207682226031E-4 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0015752596027825386 36-37 0.005418893033571933 38-39 0.0024574049803407602 40-41 0.003591591894344188 42-43 3.150519205565077E-4 44-45 0.0014492388345599355 46-47 0.0 48-49 2.520415364452062E-4 50-51 1.260207682226031E-4 52-53 2.520415364452062E-4 54-55 0.0 56-57 0.0010081661457808247 58-59 0.0020163322915616494 60-61 8.1913499344692E-4 62-63 0.0013862284504486339 64-65 1.260207682226031E-4 66-67 5.670934570017139E-4 68-69 0.0017642907551164433 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.2527346506704305 78-79 2.520415364452062E-4 80-81 1.260207682226031E-4 82-83 0.0 84-85 0.0 86-87 0.1027069261014215 88-89 0.3635699163222099 90-91 0.270188527069261 92-93 0.0 94-95 0.0 96-97 0.0 98-99 6.301038411130154E-5 100-101 6.301038411130154E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 793520.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 30.598963856387485 #Duplication Level Percentage of deduplicated Percentage of total 1 76.34694423651827 23.361373872388533 2 12.926148217140254 7.910534841971644 3 4.5329337698335745 4.161092297596074 4 2.0746961348699275 2.539342081754869 5 1.150698131016214 1.7605085260288886 6 0.6475146313648544 1.188796608096915 7 0.45016731508619856 0.964225738255471 8 0.30742338721372797 0.7525469691168861 9 0.226612569229005 0.6240698833717288 >10 1.131070447536893 6.262351847479889 >50 0.09567845524616549 2.0330843076706366 >100 0.0713464342901852 4.704449319229668 >500 0.011959806904135091 2.440811661280586 >1k 0.018970728192766007 11.215593941662426 >5k 0.0049488856155041755 10.61832573812396 >10k+ 0.002886849942377436 19.46289236597184 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 41044 5.172396410928521 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 34945 4.403795745538865 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 22551 2.841894344187922 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 16029 2.019986893840105 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 14227 1.7928974695029742 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 13612 1.7153946970460732 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 11824 1.4900695634640588 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 9832 1.2390361931646336 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9224 1.1624155660852908 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 8669 1.092474039721746 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7075 0.8915969351749169 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 7074 0.8914709144066942 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 6721 0.8469855832241153 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 6385 0.8046426051013207 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 6322 0.7967032967032966 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6169 0.7774221191652384 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5978 0.7533521524347213 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 5460 0.6880733944954129 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 5235 0.6597187216453271 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4150 0.5229861881238028 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 4093 0.5158030043351144 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 3535 0.4454834156669019 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 3511 0.44245891722955943 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3411 0.4298568404072991 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 3250 0.40956749672346004 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 3124 0.393688879927412 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 2944 0.3710051416473435 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2741 0.3454229256981551 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 2715 0.34214638572436734 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 2553 0.3217310212723057 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 2496 0.3145478374836173 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2333 0.294006452263333 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 2205 0.27787579393083983 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2117 0.26678596632725077 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 2080 0.2621231979030144 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2058 0.2593507410021172 No Hit GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT 1985 0.25015122492186714 No Hit AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG 1939 0.24435426958362738 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1827 0.23023994354269584 No Hit CTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACT 1635 0.20604395604395606 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1609 0.20276741607016835 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1512 0.19054340155257588 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1508 0.19003931847968544 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 1487 0.1873928823470108 No Hit TACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGA 1470 0.18525052928722655 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1452 0.18298215545921967 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1434 0.18071378163121282 No Hit GCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAA 1392 0.1754209093658635 No Hit GCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT 1373 0.17302651476963404 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1370 0.1726484524649662 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 1338 0.16861578788184292 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1295 0.163196894848271 No Hit TCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTA 1271 0.16017239641092854 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 1255 0.1581560641193669 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1238 0.15601371105958262 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 1228 0.15475350337735658 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 1228 0.15475350337735658 No Hit GTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA 1207 0.15210706724468193 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1119 0.14101723964109286 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1109 0.13975703195886682 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 1108 0.13963101119064422 No Hit GTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1083 0.13648049198507914 No Hit CATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1044 0.1315656820243976 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1039 0.1309355781832846 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 1006 0.12677689283193871 No Hit TTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCG 999 0.12589474745438048 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 912 0.11493094061901402 No Hit GAGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA 882 0.11115031757233593 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 879 0.11077225526766811 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 872 0.10989010989010989 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA 841 0.10598346607520918 No Hit GAGTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 828 0.10434519608831536 No Hit GTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA 797 0.10043855227341465 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.260207682226031E-4 0.0 12-13 0.0 0.0 0.0 1.8903115233390465E-4 0.0 14-15 0.0 0.0 0.0 2.520415364452062E-4 0.0 16-17 0.0 0.0 0.0 3.150519205565077E-4 0.0 18-19 0.0 0.0 0.0 3.7806230466780924E-4 0.0 20-21 0.0 0.0 0.0 9.451557616695231E-4 0.0 22-23 0.0 0.0 0.0 0.0029614880532311725 0.0 24-25 0.0 0.0 0.0 0.005481903417683234 0.0 26-27 0.0 0.0 0.0 0.008758443391470914 0.0 28-29 0.0 0.0 0.0 0.017705917935275733 0.0 30-31 0.0 6.301038411130154E-5 0.0 0.04183889504990422 0.0 32-33 0.0 1.260207682226031E-4 0.0 0.08077931243068859 0.0 34-35 0.0 1.260207682226031E-4 0.0 0.1596683133380381 0.0 36-37 0.0 1.260207682226031E-4 0.0 0.26873928823470106 0.0 38-39 0.0 1.260207682226031E-4 0.0 0.3910424437947374 0.0 40-41 0.0 1.260207682226031E-4 0.0 0.5236162919649159 0.0 42-43 0.0 1.260207682226031E-4 0.0 0.658647545115435 0.0 44-45 0.0 1.260207682226031E-4 0.0 0.7968293174715193 0.0 46-47 0.0 1.260207682226031E-4 0.0 0.9881288436334308 0.0 48-49 0.0 1.260207682226031E-4 0.0 1.2179277144873475 0.0 50-51 0.0 1.260207682226031E-4 0.0 1.43783395503579 0.0 52-53 0.0 1.260207682226031E-4 0.0 1.6263610242968043 0.0 54-55 0.0 1.260207682226031E-4 0.0 1.8228904123399536 0.0 56-57 0.0 1.260207682226031E-4 0.0 2.044245891722956 0.0 58-59 0.0 1.260207682226031E-4 0.0 2.391181066639782 0.0 60-61 0.0 1.260207682226031E-4 0.0 2.9343305776792015 0.0 62-63 0.0 1.260207682226031E-4 0.0 3.350703195886682 0.0 64-65 0.0 1.260207682226031E-4 0.0 3.657752797661055 0.0 66-67 0.0 1.260207682226031E-4 0.0 3.9973157576368585 0.0 68-69 0.0 1.260207682226031E-4 0.0 4.374684948079444 0.0 70-71 0.0 1.260207682226031E-4 0.0 4.8400166347414055 0.0 72-73 0.0 1.260207682226031E-4 0.0 5.401502167557213 0.0 74-75 0.0 1.260207682226031E-4 0.0 5.938602681721948 0.0 76-77 0.0 1.260207682226031E-4 0.0 6.393096582316765 0.0 78-79 0.0 1.260207682226031E-4 0.0 6.902913600161307 0.0 80-81 0.0 1.260207682226031E-4 0.0 7.450284806936184 0.0 82-83 0.0 1.260207682226031E-4 0.0 8.024687468494808 0.0 84-85 0.0 1.260207682226031E-4 0.0 9.015084685956246 0.0 86-87 0.0 1.260207682226031E-4 0.0 10.107180663373324 0.0 88-89 0.0 1.260207682226031E-4 0.0 10.818945962294586 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTCACT 70 7.7907316E-8 47.59702 1 CATTCCG 70 7.978633E-8 47.453056 9 GTAAGCG 100 0.0 33.217136 94-95 AGTACTC 385 0.0 30.813671 5 AAGTACT 380 0.0 29.97035 4 GTATAGG 100 4.8827977E-5 28.558208 1 AAAGTAC 390 0.0 27.985134 3 TAGGCAT 145 7.950712E-7 26.180996 5 ATAGTAC 110 9.5368945E-5 25.883484 3 ACCACTC 500 0.0 25.624651 94-95 GGGAACC 205 9.658834E-10 25.462614 7 GTCGGGA 210 1.2787496E-9 24.856361 2 GCTTATG 115 1.2636332E-4 24.833227 1 AGGCATT 145 2.351066E-5 22.908371 6 CTTTAGG 255 4.965841E-10 22.330847 2 CGGGAGT 235 4.6966306E-9 22.212069 4 GAGTGGT 150 3.0549185E-5 22.14476 9 GTCACTA 150 3.0549185E-5 22.14476 2 TTAGGCA 175 4.0814684E-6 21.692827 4 CAGCGTA 55 0.0028040418 21.68778 84-85 >>END_MODULE